AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene86453

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene86453.t1 MTR_2g015010 89.916 119 12 0 60 178 13 131 1.89e-64 204
MS.gene86453.t1 MTR_8g040300 100.000 32 0 0 195 226 113 144 1.22e-14 72.4
MS.gene86453.t1 MTR_8g035890 90.625 32 3 0 195 226 119 150 2.39e-12 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene86453.t1 AT2G36230 58.647 133 35 5 53 178 5 124 1.69e-38 137

Find 55 sgRNAs with CRISPR-Local

Find 120 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATCCTCTTTCAAATCTTTA+AGG 0.074825 5.1:+16165769 None:intergenic
TTCAAACAAGATGGACTTAA+AGG 0.177160 5.1:-16165684 MS.gene86453:CDS
TCAGGCTCAAAATTGGTAAT+TGG 0.210433 5.1:+16165735 None:intergenic
TAATGACAAGCTATGAAATA+TGG 0.284545 5.1:-16164788 MS.gene86453:CDS
ATCCTCTTTCAAATCTTTAA+GGG 0.339832 5.1:+16165770 None:intergenic
AGATTTATCAGGCTCAAAAT+TGG 0.342018 5.1:+16165728 None:intergenic
TACTCAGCTGCAGATTTATC+AGG 0.375812 5.1:+16165717 None:intergenic
AGAGGATTGAAGCGAAGAAA+AGG 0.389778 5.1:+16166016 None:intergenic
AACATTGCAAGACAGTTGTT+TGG 0.395025 5.1:-16164279 MS.gene86453:CDS
TTTGAGCAAGAAGAAATTGT+TGG 0.395461 5.1:+16166107 None:intergenic
TGAACGGAGCATTGAATAGA+AGG 0.397372 5.1:+16165965 None:intergenic
ACTTCAGCTCGCCGGTGACA+TGG 0.438078 5.1:+16164325 None:intergenic
TTGAGCAAGAAGAAATTGTT+GGG 0.456495 5.1:+16166108 None:intergenic
AGATGATTGTGGCGGAGGAA+TGG 0.460559 5.1:+16166040 None:intergenic
GAGGATTGAAGCGAAGAAAA+GGG 0.461112 5.1:+16166017 None:intergenic
GGTTGTCATGTTATCATGCT+TGG 0.465735 5.1:-16165663 MS.gene86453:CDS
AAGGGAGATGATTGTGGCGG+AGG 0.469234 5.1:+16166035 None:intergenic
CGTGTTGGTGAAGAGGAGAG+AGG 0.471556 5.1:+16165998 None:intergenic
GAAGCATTGCATGCTTATCC+TGG 0.477302 5.1:-16165609 MS.gene86453:intron
AAAGATTTGAAAGAGGATGA+TGG 0.480092 5.1:-16165765 MS.gene86453:CDS
GGAAAGTGAAACAAATTGTT+GGG 0.481539 5.1:-16165797 MS.gene86453:CDS
TCATCCTCTCTTCTCTCTCA+TGG 0.485400 5.1:-16166079 MS.gene86453:CDS
TACCTTGTGAATATCGATGC+AGG 0.487164 5.1:+16165934 None:intergenic
GCGAGAGTTGGTGCCGCCAC+AGG 0.493402 5.1:+16166166 None:intergenic
CCTCTCTTCTCTCTCATGGT+AGG 0.494725 5.1:-16166075 MS.gene86453:CDS
GGGAAAGTGAAACAAATTGT+TGG 0.500496 5.1:-16165798 MS.gene86453:CDS
ATGTCACCGGCGAGCTGAAG+TGG 0.509213 5.1:-16164323 MS.gene86453:CDS
AACCCTTAAAGATTTGAAAG+AGG 0.519640 5.1:-16165772 MS.gene86453:CDS
AAGATTTGAAAGAGGATGAT+GGG 0.525706 5.1:-16165764 MS.gene86453:CDS
CTTCAGCTCGCCGGTGACAT+GGG 0.527166 5.1:+16164326 None:intergenic
ACCTTGTGAATATCGATGCA+GGG 0.538181 5.1:+16165935 None:intergenic
CACAGGTGATGGTTGTGATA+CGG 0.542600 5.1:+16166183 None:intergenic
GTTGGTGCCGCCACAGGTGA+TGG 0.545932 5.1:+16166172 None:intergenic
AGAGAAGCAGCTTTACTCAA+AGG 0.547387 5.1:+16165633 None:intergenic
GATGCAGGGGCGGAATTGAA+CGG 0.547936 5.1:+16165949 None:intergenic
GAGCTTCCACTTCAGCTCGC+CGG 0.548722 5.1:+16164317 None:intergenic
AGAACTTTGTGATCTGTATG+AGG 0.549999 5.1:+16164351 None:intergenic
AAATTTGTTGCTTGATCACG+AGG 0.555828 5.1:-16164528 MS.gene86453:intron
GCAAGAAGAAATTGTTGGGT+CGG 0.558709 5.1:+16166112 None:intergenic
AAAGTTCTCTCCCATGTCAC+CGG 0.559302 5.1:-16164336 MS.gene86453:CDS
GGCAGAGAAGAAGATGGTGG+TGG 0.585050 5.1:+16166204 None:intergenic
GAAGAAAAGGGAGATGATTG+TGG 0.603928 5.1:+16166029 None:intergenic
CCCCTGCATCGATATTCACA+AGG 0.632190 5.1:-16165936 MS.gene86453:intron
TGATATCTCTGTGAATAACA+TGG 0.634938 5.1:+16164558 None:intergenic
CCTTGTGAATATCGATGCAG+GGG 0.636281 5.1:+16165936 None:intergenic
ATCACAACCATCACCTGTGG+CGG 0.637740 5.1:-16166179 MS.gene86453:CDS
TGATACGGCAGAGAAGAAGA+TGG 0.647907 5.1:+16166198 None:intergenic
ACGGAGCATTGAATAGAAGG+TGG 0.653974 5.1:+16165968 None:intergenic
GTCGGAATCAGAGATTGTTG+CGG 0.657278 5.1:+16166130 None:intergenic
GAAAAGGGAGATGATTGTGG+CGG 0.670342 5.1:+16166032 None:intergenic
TGTGAATATCGATGCAGGGG+CGG 0.677623 5.1:+16165939 None:intergenic
CGTATCACAACCATCACCTG+TGG 0.681993 5.1:-16166182 MS.gene86453:CDS
CCTACCATGAGAGAGAAGAG+AGG 0.693518 5.1:+16166075 None:intergenic
TACGGCAGAGAAGAAGATGG+TGG 0.731980 5.1:+16166201 None:intergenic
AATATGTCAATGCTTCCGTG+AGG 0.758709 5.1:+16164591 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAACAATTATTTCATCTA+TGG + chr5.1:16166052-16166071 None:intergenic 15.0%
!! AAAACAATTATTTCATCTAT+GGG + chr5.1:16166051-16166070 None:intergenic 15.0%
!! AAACTTACAAAAATCAATAT+AGG + chr5.1:16165514-16165533 None:intergenic 15.0%
!! CAAATTACTAAAATAAAACA+CGG + chr5.1:16165027-16165046 None:intergenic 15.0%
!!! AAAAGTTAATATTGATTGAA+AGG + chr5.1:16164522-16164541 None:intergenic 15.0%
!!! AAAGTTAATATTGATTGAAA+GGG + chr5.1:16164521-16164540 None:intergenic 15.0%
!! AAATTATAAGTAGCATAGAA+TGG + chr5.1:16165731-16165750 None:intergenic 20.0%
!! ATACACATATTTCAAACTAA+AGG - chr5.1:16165349-16165368 MS.gene86453:intron 20.0%
!! TGAACAGAAAAGTAAAAAAA+TGG + chr5.1:16164863-16164882 None:intergenic 20.0%
! AACATACATTGTGAAAAACA+TGG + chr5.1:16165700-16165719 None:intergenic 25.0%
! ACAAAAATCAATATAGGACA+TGG + chr5.1:16165508-16165527 None:intergenic 25.0%
! ATACAAAGAATTGCTACAAA+AGG + chr5.1:16165762-16165781 None:intergenic 25.0%
! ATCCTCTTTCAAATCTTTAA+GGG + chr5.1:16164661-16164680 None:intergenic 25.0%
! GATACTTTATGACACATATA+TGG + chr5.1:16165546-16165565 None:intergenic 25.0%
! TAATGACAAGCTATGAAATA+TGG - chr5.1:16165640-16165659 MS.gene86453:CDS 25.0%
! TGTAATCAAACTAAAATACC+AGG + chr5.1:16164840-16164859 None:intergenic 25.0%
!! AAGGTATATCTAAGAAGTAT+TGG + chr5.1:16165274-16165293 None:intergenic 25.0%
!! AATTTTACATGTGGGTTTTT+AGG - chr5.1:16164567-16164586 MS.gene86453:CDS 25.0%
!! ATTTTACATGTGGGTTTTTA+GGG - chr5.1:16164568-16164587 MS.gene86453:CDS 25.0%
!!! TTTCCACATTGATAATGTTT+TGG - chr5.1:16165155-16165174 MS.gene86453:intron 25.0%
AAAGATTTGAAAGAGGATGA+TGG - chr5.1:16164663-16164682 MS.gene86453:intron 30.0%
AACCCTTAAAGATTTGAAAG+AGG - chr5.1:16164656-16164675 MS.gene86453:intron 30.0%
AAGATTTGAAAGAGGATGAT+GGG - chr5.1:16164664-16164683 MS.gene86453:intron 30.0%
ACAATAAGTAGCTTTCATGT+CGG + chr5.1:16165125-16165144 None:intergenic 30.0%
AGATTTATCAGGCTCAAAAT+TGG + chr5.1:16164703-16164722 None:intergenic 30.0%
ATTATTTCATCTATGGGCAA+TGG + chr5.1:16166045-16166064 None:intergenic 30.0%
CACAAAATATCACATGTGTT+TGG + chr5.1:16165386-16165405 None:intergenic 30.0%
CATCCTCTTTCAAATCTTTA+AGG + chr5.1:16164662-16164681 None:intergenic 30.0%
GGAAAGTGAAACAAATTGTT+GGG - chr5.1:16164631-16164650 MS.gene86453:intron 30.0%
GTTCCAAAACATTATCAATG+TGG + chr5.1:16165161-16165180 None:intergenic 30.0%
TAAGCCTTCAATTGATATTG+TGG - chr5.1:16165067-16165086 MS.gene86453:intron 30.0%
TAGTCGAAGAGATGTTAAAA+CGG + chr5.1:16165988-16166007 None:intergenic 30.0%
TCTGTATCATACTATCTATC+TGG - chr5.1:16165564-16165583 MS.gene86453:intron 30.0%
TGATATCTCTGTGAATAACA+TGG + chr5.1:16165873-16165892 None:intergenic 30.0%
TTAACTTCAACTCACTATTC+TGG + chr5.1:16165187-16165206 None:intergenic 30.0%
TTGAGCAAGAAGAAATTGTT+GGG + chr5.1:16164323-16164342 None:intergenic 30.0%
TTTGAGCAAGAAGAAATTGT+TGG + chr5.1:16164324-16164343 None:intergenic 30.0%
TTTGCCAATTCATGTCAAAA+AGG - chr5.1:16164540-16164559 MS.gene86453:CDS 30.0%
! AAGGTACGAATTTTACATGT+GGG - chr5.1:16164559-16164578 MS.gene86453:CDS 30.0%
! ATTGTCATTTAACTCCACAT+AGG - chr5.1:16166218-16166237 MS.gene86453:CDS 30.0%
! TGAGTTGAAGTTAACATTGA+AGG - chr5.1:16165193-16165212 MS.gene86453:intron 30.0%
! TGATCAAGCAACAAATTTTC+AGG + chr5.1:16165898-16165917 None:intergenic 30.0%
! TGGTGTGTTTTGTAAAATTG+CGG - chr5.1:16165087-16165106 MS.gene86453:intron 30.0%
! TTAGTACATTTTAAGCCTCA+CGG - chr5.1:16165822-16165841 MS.gene86453:intron 30.0%
! TTCAAACAAGATGGACTTAA+AGG - chr5.1:16164744-16164763 MS.gene86453:intron 30.0%
!! AAAGGTACGAATTTTACATG+TGG - chr5.1:16164558-16164577 MS.gene86453:CDS 30.0%
!!! GCAATGAAATTTTAAGACGT+GGG - chr5.1:16164590-16164609 MS.gene86453:CDS 30.0%
AACATTGCAAGACAGTTGTT+TGG - chr5.1:16166149-16166168 MS.gene86453:CDS 35.0%
ACTATCTATCTGGACTTCTT+TGG - chr5.1:16165574-16165593 MS.gene86453:intron 35.0%
AGAACTTTGTGATCTGTATG+AGG + chr5.1:16166080-16166099 None:intergenic 35.0%
CACACCACAATATCAATTGA+AGG + chr5.1:16165074-16165093 None:intergenic 35.0%
CTCTGATTTCAATACCTATG+TGG + chr5.1:16166235-16166254 None:intergenic 35.0%
GATGAGATCATCAGCATAAT+TGG + chr5.1:16165964-16165983 None:intergenic 35.0%
GGGAAAGTGAAACAAATTGT+TGG - chr5.1:16164630-16164649 MS.gene86453:intron 35.0%
TGCATGGAAGGTTATCTTTA+AGG - chr5.1:16165223-16165242 MS.gene86453:intron 35.0%
! CGTACCTTTTTGACATGAAT+TGG + chr5.1:16164547-16164566 None:intergenic 35.0%
! GCTACTCTTTTCAAACAAGA+TGG - chr5.1:16164735-16164754 MS.gene86453:intron 35.0%
! TCAGGCTCAAAATTGGTAAT+TGG + chr5.1:16164696-16164715 None:intergenic 35.0%
! TGTGTTTTGTAAAATTGCGG+TGG - chr5.1:16165090-16165109 MS.gene86453:intron 35.0%
! TTTTCTGTTCATGCTTATCC+TGG - chr5.1:16164872-16164891 MS.gene86453:intron 35.0%
!! AAATTTGTTGCTTGATCACG+AGG - chr5.1:16165900-16165919 MS.gene86453:intron 35.0%
!! TGGGTCATTGTGTTTTTGTA+GGG - chr5.1:16164609-16164628 MS.gene86453:intron 35.0%
!!! GGCAATGAAATTTTAAGACG+TGG - chr5.1:16164589-16164608 MS.gene86453:CDS 35.0%
AACTTACTTTGTTGCAGGTG+GGG - chr5.1:16164979-16164998 MS.gene86453:intron 40.0%
AATATGTCAATGCTTCCGTG+AGG + chr5.1:16165840-16165859 None:intergenic 40.0%
ACAACTTACTTTGTTGCAGG+TGG - chr5.1:16164977-16164996 MS.gene86453:intron 40.0%
ACCTTGTGAATATCGATGCA+GGG + chr5.1:16164496-16164515 None:intergenic 40.0%
AGAGAAGCAGCTTTACTCAA+AGG + chr5.1:16164798-16164817 None:intergenic 40.0%
AGAGGATTGAAGCGAAGAAA+AGG + chr5.1:16164415-16164434 None:intergenic 40.0%
ATCTATCTGGACTTCTTTGG+AGG - chr5.1:16165577-16165596 MS.gene86453:intron 40.0%
CAACTTACTTTGTTGCAGGT+GGG - chr5.1:16164978-16164997 MS.gene86453:intron 40.0%
CAGTCAGAAATACGTATCGA+AGG + chr5.1:16165293-16165312 None:intergenic 40.0%
GAAGAAAAGGGAGATGATTG+TGG + chr5.1:16164402-16164421 None:intergenic 40.0%
GAGGATTGAAGCGAAGAAAA+GGG + chr5.1:16164414-16164433 None:intergenic 40.0%
GCAAGAAGAAATTGTTGGGT+CGG + chr5.1:16164319-16164338 None:intergenic 40.0%
GCGCAAGATGCAATACATTT+CGG - chr5.1:16164902-16164921 MS.gene86453:intron 40.0%
GGTTGTCATGTTATCATGCT+TGG - chr5.1:16164765-16164784 MS.gene86453:intron 40.0%
TACCTTGTGAATATCGATGC+AGG + chr5.1:16164497-16164516 None:intergenic 40.0%
TACTCAGCTGCAGATTTATC+AGG + chr5.1:16164714-16164733 None:intergenic 40.0%
TGCACAACTTACTTTGTTGC+AGG - chr5.1:16164974-16164993 MS.gene86453:intron 40.0%
! AAGTTGTGCAGAAAGTGTAG+TGG + chr5.1:16164964-16164983 None:intergenic 40.0%
!! AGATTTTCGTGTTGGTGAAG+AGG + chr5.1:16164440-16164459 None:intergenic 40.0%
!! GGGTCATTGTGTTTTTGTAG+GGG - chr5.1:16164610-16164629 MS.gene86453:intron 40.0%
!! GTGGGTCATTGTGTTTTTGT+AGG - chr5.1:16164608-16164627 MS.gene86453:intron 40.0%
!! TGAACGGAGCATTGAATAGA+AGG + chr5.1:16164466-16164485 None:intergenic 40.0%
!!! CAACTCTCGCTTTTTCATCT+CGG - chr5.1:16164275-16164294 MS.gene86453:CDS 40.0%
AAAGTTCTCTCCCATGTCAC+CGG - chr5.1:16166092-16166111 MS.gene86453:CDS 45.0%
CATTGAAGGCTGTAGTTGCA+TGG - chr5.1:16165207-16165226 MS.gene86453:intron 45.0%
CATTTCGGAGCTAGAACACA+AGG - chr5.1:16164917-16164936 MS.gene86453:intron 45.0%
CCTTGTGAATATCGATGCAG+GGG + chr5.1:16164495-16164514 None:intergenic 45.0%
CGAGATGAAAAAGCGAGAGT+TGG + chr5.1:16164277-16164296 None:intergenic 45.0%
GAAAAGGGAGATGATTGTGG+CGG + chr5.1:16164399-16164418 None:intergenic 45.0%
GTCGGAATCAGAGATTGTTG+CGG + chr5.1:16164301-16164320 None:intergenic 45.0%
TCATCCTCTCTTCTCTCTCA+TGG - chr5.1:16164349-16164368 MS.gene86453:CDS 45.0%
TGATACGGCAGAGAAGAAGA+TGG + chr5.1:16164233-16164252 None:intergenic 45.0%
! CACAGGTGATGGTTGTGATA+CGG + chr5.1:16164248-16164267 None:intergenic 45.0%
! GAAGGTGGAGATTTTCGTGT+TGG + chr5.1:16164448-16164467 None:intergenic 45.0%
!! ACGGAGCATTGAATAGAAGG+TGG + chr5.1:16164463-16164482 None:intergenic 45.0%
!! GAAGCATTGCATGCTTATCC+TGG - chr5.1:16164819-16164838 MS.gene86453:intron 45.0%
AAGTAGCTTTCATGTCGGCG+TGG + chr5.1:16165120-16165139 None:intergenic 50.0%
ATCACAACCATCACCTGTGG+CGG - chr5.1:16164249-16164268 MS.gene86453:intron 50.0%
CCTACCATGAGAGAGAAGAG+AGG + chr5.1:16164356-16164375 None:intergenic 50.0%
CCTCTCTTCTCTCTCATGGT+AGG - chr5.1:16164353-16164372 MS.gene86453:CDS 50.0%
CGTATCACAACCATCACCTG+TGG - chr5.1:16164246-16164265 MS.gene86453:intron 50.0%
GAAGGCTGTAGTTGCATGGA+AGG - chr5.1:16165211-16165230 MS.gene86453:intron 50.0%
GCATCTTGCGCTCAAATACC+AGG + chr5.1:16164893-16164912 None:intergenic 50.0%
TACGGCAGAGAAGAAGATGG+TGG + chr5.1:16164230-16164249 None:intergenic 50.0%
TGTGAATATCGATGCAGGGG+CGG + chr5.1:16164492-16164511 None:intergenic 50.0%
! AGATGATTGTGGCGGAGGAA+TGG + chr5.1:16164391-16164410 None:intergenic 50.0%
! CCCCTGCATCGATATTCACA+AGG - chr5.1:16164492-16164511 MS.gene86453:intron 50.0%
AAGGGAGATGATTGTGGCGG+AGG + chr5.1:16164396-16164415 None:intergenic 55.0%
GATGCAGGGGCGGAATTGAA+CGG + chr5.1:16164482-16164501 None:intergenic 55.0%
! CGTGTTGGTGAAGAGGAGAG+AGG + chr5.1:16164433-16164452 None:intergenic 55.0%
! GGCAGAGAAGAAGATGGTGG+TGG + chr5.1:16164227-16164246 None:intergenic 55.0%
ACTTCAGCTCGCCGGTGACA+TGG + chr5.1:16166106-16166125 None:intergenic 60.0%
ATGTCACCGGCGAGCTGAAG+TGG - chr5.1:16166105-16166124 MS.gene86453:CDS 60.0%
CTTCAGCTCGCCGGTGACAT+GGG + chr5.1:16166105-16166124 None:intergenic 60.0%
GAGCTTCCACTTCAGCTCGC+CGG + chr5.1:16166114-16166133 None:intergenic 60.0%
!! GTTGGTGCCGCCACAGGTGA+TGG + chr5.1:16164259-16164278 None:intergenic 65.0%
!! GCGAGAGTTGGTGCCGCCAC+AGG + chr5.1:16164265-16164284 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 16164193 16166257 16164193 ID=MS.gene86453
chr5.1 mRNA 16164193 16166257 16164193 ID=MS.gene86453.t1;Parent=MS.gene86453
chr5.1 exon 16165937 16166257 16165937 ID=MS.gene86453.t1.exon1;Parent=MS.gene86453.t1
chr5.1 CDS 16165937 16166257 16165937 ID=cds.MS.gene86453.t1;Parent=MS.gene86453.t1
chr5.1 exon 16165610 16165820 16165610 ID=MS.gene86453.t1.exon2;Parent=MS.gene86453.t1
chr5.1 CDS 16165610 16165820 16165610 ID=cds.MS.gene86453.t1;Parent=MS.gene86453.t1
chr5.1 exon 16164776 16164825 16164776 ID=MS.gene86453.t1.exon3;Parent=MS.gene86453.t1
chr5.1 CDS 16164776 16164825 16164776 ID=cds.MS.gene86453.t1;Parent=MS.gene86453.t1
chr5.1 exon 16164529 16164624 16164529 ID=MS.gene86453.t1.exon4;Parent=MS.gene86453.t1
chr5.1 CDS 16164529 16164624 16164529 ID=cds.MS.gene86453.t1;Parent=MS.gene86453.t1
chr5.1 exon 16164275 16164376 16164275 ID=MS.gene86453.t1.exon5;Parent=MS.gene86453.t1
chr5.1 CDS 16164275 16164376 16164275 ID=cds.MS.gene86453.t1;Parent=MS.gene86453.t1
chr5.1 exon 16164193 16164210 16164193 ID=MS.gene86453.t1.exon6;Parent=MS.gene86453.t1
chr5.1 CDS 16164193 16164210 16164193 ID=cds.MS.gene86453.t1;Parent=MS.gene86453.t1
Gene Sequence

>MS.gene86453

ATGGCACTTACGTCGTCGCAGAAGTTCTTCTCCACCACCATCTTCTTCTCTGCCGTATCACAACCATCACCTGTGGCGGCACCAACTCTCGCTTTTTCATCTCGGCCGCAACAATCTCTGATTCCGACCCAACAATTTCTTCTTGCTCAAATTAGATCATCCTCTCTTCTCTCTCATGGTAGGTTCGCATCTTCTCCATTCCTCCGCCACAATCATCTCCCTTTTCTTCGCTTCAATCCTCTCTCCTCTTCACCAACACGAAAATCTCCACCTTCTATTCAATGCTCCGTTCAATTCCGCCCCTGCATCGATATTCACAAGGTAACCCTTTCAATCAATATTAACTTTTTGCCAATTCATGTCAAAAAGGTACGAATTTTACATGTGGGTTTTTAGGGCAATGAAATTTTAAGACGTGGGTCATTGTGTTTTTGTAGGGGAAAGTGAAACAAATTGTTGGGTCAACCCTTAAAGATTTGAAAGAGGATGATGGGTCAGATCCAATTACCAATTTTGAGCCTGATAAATCTGCAGCTGAGTATGCTACTCTTTTCAAACAAGATGGACTTAAAGGTTGTCATGTTATCATGCTTGGTACTGATCCTTTGAGTAAAGCTGCTTCTCTTGAAGCATTGCATGCTTATCCTGGTATTTTAGTTTGATTACACCATTTTTTTACTTTTCTGTTCATGCTTATCCTGGTATTTGAGCGCAAGATGCAATACATTTCGGAGCTAGAACACAAGGTTCAGACTCTGCAGACTGAAGCCACTACACTTTCTGCACAACTTACTTTGTTGCAGGTGGGGACATTTATTTAAGTTTGTGAAACCGTGTTTTATTTTAGTAATTTGATCTTAGTTTCTTTTTTTACTAAGCCTTCAATTGATATTGTGGTGTGTTTTGTAAAATTGCGGTGGAAATCCACGCCGACATGAAAGCTACTTATTGTAGCTTCTTTGTTTCCACATTGATAATGTTTTGGAACTAACCAGAATAGTGAGTTGAAGTTAACATTGAAGGCTGTAGTTGCATGGAAGGTTATCTTTAAGGAACAACTTGAAAAGTACAAATTTCACCAATACTTCTTAGATATACCTTCGATACGTATTTCTGACTGACGACACATTTGTCATCTAGATACGATACATCTTTGATACACATATTTCAAACTAAAGGCATATTTCTAACCAAACACATGTGATATTTTGTGATTTGTACACAAAAAGCATTCAGTATTTGTTTTAATATCTTCGATACGTATTTCTGACTAAAGACACGATTGTCATAGTTTTTTAGTCAATGTATCTTGCCATGTCCTATATTGATTTTTGTAAGTTTCTCATATCACCATATATGTGTCATAAAGTATCTGTATCATACTATCTATCTGGACTTCTTTGGAGGTATGATATGTATGTGATCATTTTAGCAGTTGAGCTAATTGTAATGACAAGCTATGAAATATGGATTACAGAGAAAGTGATAATTTGATTTGAAACTACCATGTTTTTCACAATGTATGTTATATTCTACCATTCTATGCTACTTATAATTTTTCTCTTTCCTTTTGTAGCAATTCTTTGTATTATATTGAATCTTACTTAGTTTATTTCAACTTTGAAAATTTTAGTACATTTTAAGCCTCACGGAAGCATTGACATATTGTCACGAGAACCATGTTATTCACAGAGATATCAAGCCTGAAAATTTGTTGCTTGATCACGAGGTATCTTTCTATTAATTTACTTATATTTGAACTGCTCTTCCAATTATGCTGATGATCTCATCGCCGTTTTAACATCTCTTCGACTACTAAATTTTGTTTTCTTTTTGATAGTGTTTCGATCCATTGCCCATAGATGAAATAATTGTTTTTAGTTGCCTCATACAGATCACAAAGTTCTCTCCCATGTCACCGGCGAGCTGAAGTGGAAGCTCTATGAGAGAAGACGAAACATTGCAAGACAGTTGTTTGGAGCTGTTTGTATAGTAACAATTTTTTTAAGATTTGACTTATGTCTAATTGTCATTTAACTCCACATAGGTATTGAAATCAGAGTGA
Protein sequence

>MS.gene86453.t1

MALTSSQKFFSTTIFFSAVSQPSPVAAPTLAFSSRPQQSLIPTQQFLLAQIRSSSLLSHGRFASSPFLRHNHLPFLRFNPLSSSPTRKSPPSIQCSVQFRPCIDIHKGKVKQIVGSTLKDLKEDDGSDPITNFEPDKSAAEYATLFKQDGLKGCHVIMLGTDPLSKAASLEALHAYPAVELIVMTSYEIWITEKYILSLTEALTYCHENHVIHRDIKPENLLLDHELPHTDHKVLSHVTGELKWKLYERRRNIARQLFGAVLKSE