AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene86529

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene86529.t1 MTR_5g024390 93.692 428 27 0 1 428 1 428 0.0 831
MS.gene86529.t1 MTR_5g024500 92.523 428 32 0 1 428 1 428 0.0 822
MS.gene86529.t1 MTR_3g103110 68.103 464 108 4 3 428 339 800 0.0 655
MS.gene86529.t1 MTR_7g109640 71.233 438 89 4 3 428 384 796 0.0 644
MS.gene86529.t1 MTR_3g103120 66.743 439 112 3 2 428 425 841 0.0 583
MS.gene86529.t1 MTR_5g071340 43.187 433 207 9 3 421 679 1086 1.17e-112 356
MS.gene86529.t1 MTR_3g464550 43.056 432 209 9 1 419 601 1008 2.82e-109 347
MS.gene86529.t1 MTR_2g078040 34.266 429 246 8 3 419 762 1166 5.79e-72 246
MS.gene86529.t1 MTR_3g103160 39.007 423 164 12 2 421 107 438 1.82e-71 232
MS.gene86529.t1 MTR_1g103830 34.965 429 245 5 3 419 606 1012 1.30e-68 236
MS.gene86529.t1 MTR_1g072130 32.353 408 244 6 3 398 812 1199 2.45e-60 213
MS.gene86529.t1 MTR_7g095140 31.579 304 196 5 125 425 13 307 1.54e-49 172
MS.gene86529.t1 MTR_4g099530 27.442 430 257 13 3 419 111 498 5.51e-40 150
MS.gene86529.t1 MTR_1g068930 25.173 433 240 12 36 419 495 892 1.68e-39 151
MS.gene86529.t1 MTR_1g068930 25.173 433 240 12 36 419 458 855 1.89e-39 151
MS.gene86529.t1 MTR_7g102610 24.468 470 237 14 29 426 527 950 1.07e-31 128
MS.gene86529.t1 MTR_4g056990 24.274 482 268 15 2 428 189 628 2.04e-31 127
MS.gene86529.t1 MTR_4g057290 25.352 426 213 10 37 394 126 514 4.14e-29 120
MS.gene86529.t1 MTR_4g050090 23.265 490 253 13 12 422 199 644 1.95e-27 115
MS.gene86529.t1 MTR_1g062330 22.727 440 264 13 37 421 386 804 1.12e-21 98.2
MS.gene86529.t1 MTR_6g061000 50.000 90 34 2 88 177 23 101 2.81e-19 82.8
MS.gene86529.t1 MTR_2g084425 30.556 180 116 4 245 423 847 1018 2.41e-17 85.1
MS.gene86529.t1 MTR_2g084425 23.529 289 139 6 12 251 470 725 2.74e-14 75.5
MS.gene86529.t1 MTR_2g084425 23.529 289 139 6 12 251 470 725 3.93e-14 75.1
MS.gene86529.t1 MTR_3g109988 29.891 184 100 6 217 390 191 355 3.06e-13 70.9
MS.gene86529.t1 MTR_3g110005 29.891 184 100 6 217 390 191 355 3.06e-13 70.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene86529.t1 AT5G58610 40.783 434 216 7 3 419 368 777 4.48e-98 312
MS.gene86529.t1 AT5G58610 40.783 434 216 7 3 419 599 1008 1.15e-96 311
MS.gene86529.t1 AT5G58610 40.783 434 216 7 3 419 599 1008 1.15e-96 311
MS.gene86529.t1 AT5G58610 40.625 448 219 9 3 428 590 1012 2.02e-96 312
MS.gene86529.t1 AT5G58610 40.625 448 219 9 3 428 599 1021 2.65e-96 312
MS.gene86529.t1 AT5G58610 40.625 448 219 9 3 428 590 1012 3.53e-96 311
MS.gene86529.t1 AT5G58610 38.902 437 216 8 3 428 590 986 2.47e-86 284
MS.gene86529.t1 AT5G58610 38.902 437 216 8 3 428 590 986 3.14e-86 284
MS.gene86529.t1 AT5G58610 40.576 382 186 7 3 367 590 947 1.61e-81 270
MS.gene86529.t1 AT5G58610 40.576 382 186 7 3 367 599 956 1.86e-81 270
MS.gene86529.t1 AT5G58610 39.826 344 166 7 3 329 590 909 1.67e-67 231
MS.gene86529.t1 AT5G36740 32.401 429 254 6 3 419 545 949 4.29e-67 232
MS.gene86529.t1 AT5G36740 32.401 429 254 6 3 419 545 949 4.29e-67 232
MS.gene86529.t1 AT3G14980 32.102 433 260 7 3 425 621 1029 3.97e-65 226
MS.gene86529.t1 AT3G14980 32.102 433 260 7 3 425 576 984 6.80e-65 225
MS.gene86529.t1 AT3G14980 32.102 433 260 7 3 425 621 1029 7.33e-65 225
MS.gene86529.t1 AT3G14980 32.102 433 260 7 3 425 621 1029 7.33e-65 225
MS.gene86529.t1 AT3G14980 32.102 433 260 7 3 425 621 1029 7.33e-65 225
MS.gene86529.t1 AT1G05380 33.021 427 255 6 3 419 520 925 1.01e-63 222
MS.gene86529.t1 AT1G05380 33.021 427 255 6 3 419 520 925 1.01e-63 222
MS.gene86529.t1 AT1G05380 33.021 427 255 6 3 419 520 925 1.01e-63 222
MS.gene86529.t1 AT5G36740 31.435 439 255 7 3 419 392 806 1.28e-63 221
MS.gene86529.t1 AT5G63900 36.858 331 190 8 94 421 228 542 2.69e-62 211
MS.gene86529.t1 AT4G14920 31.019 432 261 6 3 422 580 986 7.69e-60 211
MS.gene86529.t1 AT4G14920 31.019 432 261 6 3 422 580 986 8.69e-60 211
MS.gene86529.t1 AT4G14920 31.019 432 261 6 3 422 580 986 8.69e-60 211
MS.gene86529.t1 AT5G36670 30.337 445 256 8 3 419 545 963 1.13e-58 207
MS.gene86529.t1 AT5G36670 30.337 445 256 8 3 419 545 963 1.13e-58 207
MS.gene86529.t1 AT5G36740 32.345 371 215 6 3 361 545 891 5.66e-57 201
MS.gene86529.t1 AT5G36740 32.345 371 215 6 3 361 545 891 5.66e-57 201
MS.gene86529.t1 AT5G36670 32.345 371 215 6 3 361 545 891 5.66e-57 201
MS.gene86529.t1 AT5G36670 29.451 455 257 9 3 419 392 820 5.21e-55 197
MS.gene86529.t1 AT5G36670 29.451 455 257 9 3 419 545 973 1.24e-54 196
MS.gene86529.t1 AT3G14980 31.065 338 199 7 3 330 621 934 1.56e-43 163
MS.gene86529.t1 AT3G53680 28.261 460 241 15 13 417 405 830 6.72e-40 152
MS.gene86529.t1 AT3G53680 28.261 460 241 15 13 417 403 828 7.28e-40 152
MS.gene86529.t1 AT2G37520 25.910 467 244 12 13 417 386 812 9.26e-40 152
MS.gene86529.t1 AT2G36720 25.470 479 251 14 13 423 533 973 1.00e-38 149
MS.gene86529.t1 AT2G36720 25.470 479 251 14 13 423 533 973 1.00e-38 149
MS.gene86529.t1 AT2G27980 24.187 492 253 16 10 422 610 1060 1.98e-31 127

Find 69 sgRNAs with CRISPR-Local

Find 85 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGATTATCTGCAACCTTAAT+TGG 0.181951 5.1:-15159839 None:intergenic
TTCACATTCTTGTTTGCATT+TGG 0.183779 5.1:-15159654 None:intergenic
TGGTTTCTATAATGTAATTT+TGG 0.224054 5.1:+15160054 MS.gene86529:CDS
GTTTGTTCAGTTTGTGGTTT+TGG 0.236361 5.1:+15159509 MS.gene86529:CDS
GTGGCAACTATAAGGATTTA+TGG 0.245404 5.1:+15160100 MS.gene86529:CDS
ATCAACTCTCTTCCTGATAA+AGG 0.280267 5.1:-15159980 None:intergenic
TCACATTCTTGTTTGCATTT+GGG 0.312904 5.1:-15159653 None:intergenic
GCATTCCACCTCCGTTGTTT+AGG 0.320877 5.1:+15159566 MS.gene86529:CDS
GTTGATAACGAATCAAGTTT+TGG 0.327450 5.1:+15159145 MS.gene86529:CDS
TGATTTCAGTGGCAACTATA+AGG 0.339394 5.1:+15160092 MS.gene86529:CDS
TTATGTCTAAAGAAGTTGTT+AGG 0.347395 5.1:+15159812 MS.gene86529:CDS
AAAGGAGAGTAAGTTGAATA+TGG 0.354999 5.1:+15159916 MS.gene86529:CDS
AATTTAATCACATGGAGAAT+TGG 0.356404 5.1:+15159759 MS.gene86529:CDS
AAACGATGGGTATTGTTTCT+TGG 0.373623 5.1:+15159123 None:intergenic
GATAGTGTTTGTTCAGTTTG+TGG 0.378867 5.1:+15159503 MS.gene86529:CDS
CTGGGTTCGCATGATCATAA+AGG 0.384834 5.1:+15159374 MS.gene86529:CDS
GGATTGTCAAAGGGAAGCTC+TGG 0.400874 5.1:+15159355 MS.gene86529:CDS
AACACTTGGACAAGATCATT+TGG 0.403595 5.1:+15160271 MS.gene86529:CDS
TGAAGAGGAGTTTAATCAAA+AGG 0.404213 5.1:+15159898 MS.gene86529:CDS
AAGCACTGAAAAGACCTATA+TGG 0.410177 5.1:+15160377 MS.gene86529:CDS
CAAGCTCGCTTGAACTTTCG+TGG 0.419956 5.1:+15160034 MS.gene86529:CDS
GGCTCTTGGTGTATTGTATG+AGG 0.423950 5.1:+15159937 MS.gene86529:CDS
ACCATCCACAAGTATCTTGT+TGG 0.424383 5.1:+15159313 MS.gene86529:CDS
GTTGTTAGGAAAACCAATTA+AGG 0.435095 5.1:+15159826 MS.gene86529:CDS
ATTGAACACAAACAAGAATC+TGG 0.437308 5.1:-15159691 None:intergenic
AGTAACAAAGTTGTCAAAAG+AGG 0.441397 5.1:+15159197 MS.gene86529:CDS
ATCGAGTTCCGCTTGGTGAA+TGG 0.464422 5.1:+15159594 MS.gene86529:CDS
CAAGCAATGCAAGGCCATAT+AGG 0.465235 5.1:-15160391 None:intergenic
CCTGATAAAGGATCAAAAGT+TGG 0.477884 5.1:-15159968 None:intergenic
ACAAGTATCTTGTTGGAAGA+TGG 0.480189 5.1:+15159320 MS.gene86529:CDS
TTGTCTTCGATACTGTTCCT+TGG 0.482098 5.1:-15160155 None:intergenic
GGAAGATGGAAGGTCGTTGT+TGG 0.488980 5.1:+15159334 MS.gene86529:CDS
GCTCTTGGTGTATTGTATGA+GGG 0.493509 5.1:+15159938 MS.gene86529:CDS
TGGGTTCGCATGATCATAAA+GGG 0.494630 5.1:+15159375 MS.gene86529:CDS
GAGAAACAGTTAACTCGCTT+AGG 0.495020 5.1:+15160208 MS.gene86529:CDS
AGGAATGTGTCATTTGTTGA+TGG 0.496868 5.1:+15160177 MS.gene86529:CDS
CAGAGGATGCTATGAACACT+TGG 0.500216 5.1:+15160257 MS.gene86529:CDS
GTGATTAAATTCAATTCCTA+CGG 0.512914 5.1:-15159747 None:intergenic
GTCGTTGTTGGATTGTCAAA+GGG 0.517227 5.1:+15159346 MS.gene86529:CDS
TCTTCGATACTGTTCCTTGG+TGG 0.524421 5.1:-15160152 None:intergenic
GGTCGTTGTTGGATTGTCAA+AGG 0.528790 5.1:+15159345 MS.gene86529:CDS
AGTAAGTTGAATATGGCTCT+TGG 0.540429 5.1:+15159923 MS.gene86529:CDS
ATCTTCCAACAAGATACTTG+TGG 0.550941 5.1:-15159318 None:intergenic
GGTCTCAATCGAGTTCCGCT+TGG 0.551276 5.1:+15159587 MS.gene86529:CDS
ATATCACTTTGGATGCGTAA+AGG 0.554258 5.1:+15159724 MS.gene86529:CDS
TGTGAATAGTGTTGATGAAG+AGG 0.557314 5.1:+15159883 MS.gene86529:CDS
ACCGGCTTCGAGTCTCATGC+AGG 0.558836 5.1:+15159275 MS.gene86529:CDS
GTAACAAAGTTGTCAAAAGA+GGG 0.581494 5.1:+15159198 MS.gene86529:CDS
TGAGACCTAAACAACGGAGG+TGG 0.586505 5.1:-15159571 None:intergenic
GATTGTCAAAGGGAAGCTCT+GGG 0.597706 5.1:+15159356 MS.gene86529:CDS
AGGAATTGAATTTAATCACA+TGG 0.598130 5.1:+15159751 MS.gene86529:CDS
TTTCAGTGCTTTGAGACACA+TGG 0.604056 5.1:-15160365 None:intergenic
AGATAATCTAACATGGACAT+TGG 0.607457 5.1:+15159853 MS.gene86529:CDS
CTCCAACATCAAGCAATGCA+AGG 0.609626 5.1:-15160400 None:intergenic
TCCAACAAGATACTTGTGGA+TGG 0.616702 5.1:-15159314 None:intergenic
CAACGGAGGTGGAATGCAGA+AGG 0.622944 5.1:-15159560 None:intergenic
GTATCTTGTTGGAAGATGGA+AGG 0.629827 5.1:+15159324 MS.gene86529:CDS
ATTACTTCTGCATTCCTCAG+AGG 0.633654 5.1:+15160240 MS.gene86529:CDS
ACAAGAATGTGAAGATCACA+AGG 0.637763 5.1:+15159664 MS.gene86529:CDS
AATGGGTGAAGTGATTTCAG+TGG 0.642896 5.1:+15160081 MS.gene86529:CDS
TTTGGATGCGTAAAGGCCGT+AGG 0.643030 5.1:+15159731 MS.gene86529:CDS
ACCTGCATGAGACTCGAAGC+CGG 0.643864 5.1:-15159276 None:intergenic
AGGTTGCAGATAATCTAACA+TGG 0.665981 5.1:+15159846 MS.gene86529:CDS
GGACAAAACCATTCACCAAG+CGG 0.675521 5.1:-15159602 None:intergenic
CTCGATTGAGACCTAAACAA+CGG 0.677857 5.1:-15159577 None:intergenic
AAGGAACAGTATCGAAGACA+AGG 0.678537 5.1:+15160157 MS.gene86529:CDS
ACTACCCTGACAGAAAACAT+TGG 0.690433 5.1:-15159177 None:intergenic
AGTGTTCATAGCATCCTCTG+AGG 0.736105 5.1:-15160254 None:intergenic
GATTGAGACCTAAACAACGG+AGG 0.762353 5.1:-15159574 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATGTAATTTTGGAGAAAAT+GGG + chr5.1:15160064-15160083 MS.gene86529:CDS 20.0%
!!! ACTTTTTTAGAATTTGAGAA+TGG + chr5.1:15160340-15160359 MS.gene86529:CDS 20.0%
!!! TAATGTAATTTTGGAGAAAA+TGG + chr5.1:15160063-15160082 MS.gene86529:CDS 20.0%
!!! TGGTTTCTATAATGTAATTT+TGG + chr5.1:15160054-15160073 MS.gene86529:CDS 20.0%
! AATTTAATCACATGGAGAAT+TGG + chr5.1:15159759-15159778 MS.gene86529:CDS 25.0%
! AGGAATTGAATTTAATCACA+TGG + chr5.1:15159751-15159770 MS.gene86529:CDS 25.0%
! GTGATTAAATTCAATTCCTA+CGG - chr5.1:15159750-15159769 None:intergenic 25.0%
! TTATGTCTAAAGAAGTTGTT+AGG + chr5.1:15159812-15159831 MS.gene86529:CDS 25.0%
AGATAATCTAACATGGACAT+TGG + chr5.1:15159853-15159872 MS.gene86529:CDS 30.0%
AGATTATCTGCAACCTTAAT+TGG - chr5.1:15159842-15159861 None:intergenic 30.0%
AGTAACAAAGTTGTCAAAAG+AGG + chr5.1:15159197-15159216 MS.gene86529:CDS 30.0%
ATTGAACACAAACAAGAATC+TGG - chr5.1:15159694-15159713 None:intergenic 30.0%
GTAACAAAGTTGTCAAAAGA+GGG + chr5.1:15159198-15159217 MS.gene86529:CDS 30.0%
GTTGTTAGGAAAACCAATTA+AGG + chr5.1:15159826-15159845 MS.gene86529:CDS 30.0%
TGAAGAGGAGTTTAATCAAA+AGG + chr5.1:15159898-15159917 MS.gene86529:CDS 30.0%
TGTGAGCAAAAATATCACTT+TGG + chr5.1:15159713-15159732 MS.gene86529:CDS 30.0%
! AAAGGAGAGTAAGTTGAATA+TGG + chr5.1:15159916-15159935 MS.gene86529:CDS 30.0%
! CATATTTTGCAACAACAAGT+TGG - chr5.1:15159626-15159645 None:intergenic 30.0%
! TCACATTCTTGTTTGCATTT+GGG - chr5.1:15159656-15159675 None:intergenic 30.0%
! TTCACATTCTTGTTTGCATT+TGG - chr5.1:15159657-15159676 None:intergenic 30.0%
!!! GTTGATAACGAATCAAGTTT+TGG + chr5.1:15159145-15159164 MS.gene86529:CDS 30.0%
AACACTTGGACAAGATCATT+TGG + chr5.1:15160271-15160290 MS.gene86529:CDS 35.0%
ACAAGAATGTGAAGATCACA+AGG + chr5.1:15159664-15159683 MS.gene86529:CDS 35.0%
ACAAGTATCTTGTTGGAAGA+TGG + chr5.1:15159320-15159339 MS.gene86529:CDS 35.0%
AGGTTGCAGATAATCTAACA+TGG + chr5.1:15159846-15159865 MS.gene86529:CDS 35.0%
ATATCACTTTGGATGCGTAA+AGG + chr5.1:15159724-15159743 MS.gene86529:CDS 35.0%
ATCAACTCTCTTCCTGATAA+AGG - chr5.1:15159983-15160002 None:intergenic 35.0%
ATCTTCCAACAAGATACTTG+TGG - chr5.1:15159321-15159340 None:intergenic 35.0%
CCTGATAAAGGATCAAAAGT+TGG - chr5.1:15159971-15159990 None:intergenic 35.0%
GATAGTGTTTGTTCAGTTTG+TGG + chr5.1:15159503-15159522 MS.gene86529:CDS 35.0%
GTGGCAACTATAAGGATTTA+TGG + chr5.1:15160100-15160119 MS.gene86529:CDS 35.0%
TGATTTCAGTGGCAACTATA+AGG + chr5.1:15160092-15160111 MS.gene86529:CDS 35.0%
! AGTAAGTTGAATATGGCTCT+TGG + chr5.1:15159923-15159942 MS.gene86529:CDS 35.0%
! CCAACTTTTGATCCTTTATC+AGG + chr5.1:15159968-15159987 MS.gene86529:CDS 35.0%
! GTTTGTTCAGTTTGTGGTTT+TGG + chr5.1:15159509-15159528 MS.gene86529:CDS 35.0%
!! AAGCACTGAAAAGACCTATA+TGG + chr5.1:15160377-15160396 MS.gene86529:CDS 35.0%
!! AGGAATGTGTCATTTGTTGA+TGG + chr5.1:15160177-15160196 MS.gene86529:CDS 35.0%
!! TGTGAATAGTGTTGATGAAG+AGG + chr5.1:15159883-15159902 MS.gene86529:CDS 35.0%
!!! ACGAATCAAGTTTTGGTTTC+AGG + chr5.1:15159152-15159171 MS.gene86529:CDS 35.0%
!!! CAAGATCATTTGGTTTTGCA+AGG + chr5.1:15160281-15160300 MS.gene86529:CDS 35.0%
!!! CGAATCAAGTTTTGGTTTCA+GGG + chr5.1:15159153-15159172 MS.gene86529:CDS 35.0%
!!! CGTGTTACTTTTACTATCAC+CGG + chr5.1:15159257-15159276 MS.gene86529:CDS 35.0%
AATGGGTGAAGTGATTTCAG+TGG + chr5.1:15160081-15160100 MS.gene86529:CDS 40.0%
ACCATCCACAAGTATCTTGT+TGG + chr5.1:15159313-15159332 MS.gene86529:CDS 40.0%
ACTACCCTGACAGAAAACAT+TGG - chr5.1:15159180-15159199 None:intergenic 40.0%
ATTACTTCTGCATTCCTCAG+AGG + chr5.1:15160240-15160259 MS.gene86529:CDS 40.0%
CTCGATTGAGACCTAAACAA+CGG - chr5.1:15159580-15159599 None:intergenic 40.0%
GAGAAACAGTTAACTCGCTT+AGG + chr5.1:15160208-15160227 MS.gene86529:CDS 40.0%
GTATCTTGTTGGAAGATGGA+AGG + chr5.1:15159324-15159343 MS.gene86529:CDS 40.0%
GTCGTTGTTGGATTGTCAAA+GGG + chr5.1:15159346-15159365 MS.gene86529:CDS 40.0%
TCCAACAAGATACTTGTGGA+TGG - chr5.1:15159317-15159336 None:intergenic 40.0%
TGGGTTCGCATGATCATAAA+GGG + chr5.1:15159375-15159394 MS.gene86529:CDS 40.0%
TTGTCTTCGATACTGTTCCT+TGG - chr5.1:15160158-15160177 None:intergenic 40.0%
! AAGGAACAGTATCGAAGACA+AGG + chr5.1:15160157-15160176 MS.gene86529:CDS 40.0%
! AGTTGCAGAGATAGTTTTCG+TGG + chr5.1:15160129-15160148 MS.gene86529:CDS 40.0%
! GCTCTTGGTGTATTGTATGA+GGG + chr5.1:15159938-15159957 MS.gene86529:CDS 40.0%
!! GGTTTTGCAGTGTAGTTTGT+GGG + chr5.1:15159780-15159799 MS.gene86529:CDS 40.0%
!! TGGTTTTGCAGTGTAGTTTG+TGG + chr5.1:15159779-15159798 MS.gene86529:CDS 40.0%
!! TTTCAGTGCTTTGAGACACA+TGG - chr5.1:15160368-15160387 None:intergenic 40.0%
!!! TGTTCAGTTTGTGGTTTTGG+AGG + chr5.1:15159512-15159531 MS.gene86529:CDS 40.0%
AGTGTTCATAGCATCCTCTG+AGG - chr5.1:15160257-15160276 None:intergenic 45.0%
CAAGCAATGCAAGGCCATAT+AGG - chr5.1:15160394-15160413 None:intergenic 45.0%
CAGAGGATGCTATGAACACT+TGG + chr5.1:15160257-15160276 MS.gene86529:CDS 45.0%
CTGGGTTCGCATGATCATAA+AGG + chr5.1:15159374-15159393 MS.gene86529:CDS 45.0%
GATTGAGACCTAAACAACGG+AGG - chr5.1:15159577-15159596 None:intergenic 45.0%
GATTGTCAAAGGGAAGCTCT+GGG + chr5.1:15159356-15159375 MS.gene86529:CDS 45.0%
GGACAAAACCATTCACCAAG+CGG - chr5.1:15159605-15159624 None:intergenic 45.0%
GGTCGTTGTTGGATTGTCAA+AGG + chr5.1:15159345-15159364 MS.gene86529:CDS 45.0%
TCTTCGATACTGTTCCTTGG+TGG - chr5.1:15160155-15160174 None:intergenic 45.0%
! AGGGACCAATGTTTTCTGTC+AGG + chr5.1:15159172-15159191 MS.gene86529:CDS 45.0%
! GGCTCTTGGTGTATTGTATG+AGG + chr5.1:15159937-15159956 MS.gene86529:CDS 45.0%
! GGGACCAATGTTTTCTGTCA+GGG + chr5.1:15159173-15159192 MS.gene86529:CDS 45.0%
!!! TTGTGGTTTTGGAGGTGACT+TGG + chr5.1:15159520-15159539 MS.gene86529:CDS 45.0%
CAAGCTCGCTTGAACTTTCG+TGG + chr5.1:15160034-15160053 MS.gene86529:CDS 50.0%
GCATTCCACCTCCGTTGTTT+AGG + chr5.1:15159566-15159585 MS.gene86529:CDS 50.0%
GGAAGATGGAAGGTCGTTGT+TGG + chr5.1:15159334-15159353 MS.gene86529:CDS 50.0%
GGATTGTCAAAGGGAAGCTC+TGG + chr5.1:15159355-15159374 MS.gene86529:CDS 50.0%
TGAGACCTAAACAACGGAGG+TGG - chr5.1:15159574-15159593 None:intergenic 50.0%
TTTGGATGCGTAAAGGCCGT+AGG + chr5.1:15159731-15159750 MS.gene86529:CDS 50.0%
! ATCGAGTTCCGCTTGGTGAA+TGG + chr5.1:15159594-15159613 MS.gene86529:CDS 50.0%
! GATAGTTTTCGTGGCCACCA+AGG + chr5.1:15160138-15160157 MS.gene86529:CDS 50.0%
ACCTGCATGAGACTCGAAGC+CGG - chr5.1:15159279-15159298 None:intergenic 55.0%
CAACGGAGGTGGAATGCAGA+AGG - chr5.1:15159563-15159582 None:intergenic 55.0%
GGTCTCAATCGAGTTCCGCT+TGG + chr5.1:15159587-15159606 MS.gene86529:CDS 55.0%
! ACCGGCTTCGAGTCTCATGC+AGG + chr5.1:15159275-15159294 MS.gene86529:CDS 60.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 15159128 15160414 15159128 ID=MS.gene86529
chr5.1 mRNA 15159128 15160414 15159128 ID=MS.gene86529.t1;Parent=MS.gene86529
chr5.1 exon 15159128 15160414 15159128 ID=MS.gene86529.t1.exon1;Parent=MS.gene86529.t1
chr5.1 CDS 15159128 15160414 15159128 ID=cds.MS.gene86529.t1;Parent=MS.gene86529.t1
Gene Sequence

>MS.gene86529

ATGGGTATTGTTTCTTGGTTGATAACGAATCAAGTTTTGGTTTCAGGGACCAATGTTTTCTGTCAGGGTAGTAACAAAGTTGTCAAAAGAGGGAGTTTGTTTTCTGATGCTATAGTCTGTGATTGTTGTCGTGTTACTTTTACTATCACCGGCTTCGAGTCTCATGCAGGTTGTACAAGACATAGACCATCCACAAGTATCTTGTTGGAAGATGGAAGGTCGTTGTTGGATTGTCAAAGGGAAGCTCTGGGTTCGCATGATCATAAAGGGAATCATTGTGTTGTCAAAGAAAATCAAAAGAAGAATCATTCTATTGTCAAAGAAAATCGAAAGAAGAATCATTGTGCTGTCAAAGAAAAATCTGAAGCAAATAACGATAGTGTTTGTTCAGTTTGTGGTTTTGGAGGTGACTTGGCACTATGCGATCGATGTCCTTCTGCATTCCACCTCCGTTGTTTAGGTCTCAATCGAGTTCCGCTTGGTGAATGGTTTTGTCCAACTTGTTGTTGCAAAATATGCTATCGACCCAAATGCAAACAAGAATGTGAAGATCACAAGGATAACCAGATTCTTGTTTGTGTTCAATGTGAGCAAAAATATCACTTTGGATGCGTAAAGGCCGTAGGAATTGAATTTAATCACATGGAGAATTGGTTTTGCAGTGTAGTTTGTGGGAACATGTTCTTATGTCTAAAGAAGTTGTTAGGAAAACCAATTAAGGTTGCAGATAATCTAACATGGACATTGGTGAAGAATGTGAATAGTGTTGATGAAGAGGAGTTTAATCAAAAGGAGAGTAAGTTGAATATGGCTCTTGGTGTATTGTATGAGGGTTTTAATCCAACTTTTGATCCTTTATCAGGAAGAGAGTTGATTAAAGATGTTGTTTTTAGTAGAGAATCAGAACAAGCTCGCTTGAACTTTCGTGGTTTCTATAATGTAATTTTGGAGAAAATGGGTGAAGTGATTTCAGTGGCAACTATAAGGATTTATGGTCAAAAAGTTGCAGAGATAGTTTTCGTGGCCACCAAGGAACAGTATCGAAGACAAGGAATGTGTCATTTGTTGATGGATGAGATTGAGAAACAGTTAACTCGCTTAGGAGTTGAGAAATTACTTCTGCATTCCTCAGAGGATGCTATGAACACTTGGACAAGATCATTTGGTTTTGCAAGGATGAATAGTAAAGATAAGTGTCAGTTTATTGATCATACTTTTTTAGAATTTGAGAATGGTACCATGTGTCTCAAAGCACTGAAAAGACCTATATGGCCTTGCATTGCTTGA
Protein sequence

>MS.gene86529.t1

MGIVSWLITNQVLVSGTNVFCQGSNKVVKRGSLFSDAIVCDCCRVTFTITGFESHAGCTRHRPSTSILLEDGRSLLDCQREALGSHDHKGNHCVVKENQKKNHSIVKENRKKNHCAVKEKSEANNDSVCSVCGFGGDLALCDRCPSAFHLRCLGLNRVPLGEWFCPTCCCKICYRPKCKQECEDHKDNQILVCVQCEQKYHFGCVKAVGIEFNHMENWFCSVVCGNMFLCLKKLLGKPIKVADNLTWTLVKNVNSVDEEEFNQKESKLNMALGVLYEGFNPTFDPLSGRELIKDVVFSRESEQARLNFRGFYNVILEKMGEVISVATIRIYGQKVAEIVFVATKEQYRRQGMCHLLMDEIEKQLTRLGVEKLLLHSSEDAMNTWTRSFGFARMNSKDKCQFIDHTFLEFENGTMCLKALKRPIWPCIA