AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene86531

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene86531.t1 MTR_5g024390 93.692 428 27 0 1 428 1 428 0.0 832
MS.gene86531.t1 MTR_5g024500 92.523 428 32 0 1 428 1 428 0.0 822
MS.gene86531.t1 MTR_3g103110 68.319 464 107 4 3 428 339 800 0.0 657
MS.gene86531.t1 MTR_7g109640 71.461 438 88 4 3 428 384 796 0.0 646
MS.gene86531.t1 MTR_3g103120 66.970 439 111 3 2 428 425 841 0.0 585
MS.gene86531.t1 MTR_5g071340 43.187 433 207 9 3 421 679 1086 9.78e-113 357
MS.gene86531.t1 MTR_3g464550 43.056 432 209 9 1 419 601 1008 2.39e-109 347
MS.gene86531.t1 MTR_3g103160 39.007 423 164 12 2 421 107 438 2.05e-71 232
MS.gene86531.t1 MTR_2g078040 34.033 429 247 8 3 419 762 1166 2.81e-71 244
MS.gene86531.t1 MTR_1g103830 34.965 429 245 5 3 419 606 1012 2.09e-68 236
MS.gene86531.t1 MTR_1g072130 32.598 408 243 6 3 398 812 1199 4.98e-61 214
MS.gene86531.t1 MTR_7g095140 31.579 304 196 5 125 425 13 307 5.00e-50 173
MS.gene86531.t1 MTR_4g099530 27.442 430 257 13 3 419 111 498 1.55e-40 151
MS.gene86531.t1 MTR_1g068930 25.173 433 240 12 36 419 495 892 7.92e-40 152
MS.gene86531.t1 MTR_1g068930 25.173 433 240 12 36 419 458 855 8.80e-40 152
MS.gene86531.t1 MTR_7g102610 24.468 470 237 14 29 426 527 950 5.48e-32 129
MS.gene86531.t1 MTR_4g056990 24.274 482 268 15 2 428 189 628 3.89e-31 126
MS.gene86531.t1 MTR_4g057290 25.587 426 212 10 37 394 126 514 1.74e-29 120
MS.gene86531.t1 MTR_4g050090 23.469 490 252 13 12 422 199 644 7.75e-28 117
MS.gene86531.t1 MTR_1g062330 23.356 441 260 15 37 421 386 804 6.25e-22 99.0
MS.gene86531.t1 MTR_6g061000 50.000 90 34 2 88 177 23 101 2.72e-19 83.2
MS.gene86531.t1 MTR_2g084425 30.556 180 116 4 245 423 847 1018 1.37e-17 85.9
MS.gene86531.t1 MTR_2g084425 23.529 289 139 6 12 251 470 725 2.72e-14 75.5
MS.gene86531.t1 MTR_2g084425 23.529 289 139 6 12 251 470 725 3.66e-14 75.1
MS.gene86531.t1 MTR_3g109988 29.891 184 100 6 217 390 191 355 5.28e-13 70.5
MS.gene86531.t1 MTR_3g110005 29.891 184 100 6 217 390 191 355 5.28e-13 70.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene86531.t1 AT5G58610 41.014 434 215 7 3 419 368 777 1.05e-98 314
MS.gene86531.t1 AT5G58610 41.014 434 215 7 3 419 599 1008 2.49e-97 313
MS.gene86531.t1 AT5G58610 41.014 434 215 7 3 419 599 1008 2.49e-97 313
MS.gene86531.t1 AT5G58610 40.848 448 218 9 3 428 590 1012 4.10e-97 314
MS.gene86531.t1 AT5G58610 40.848 448 218 9 3 428 599 1021 5.79e-97 313
MS.gene86531.t1 AT5G58610 40.848 448 218 9 3 428 590 1012 6.82e-97 313
MS.gene86531.t1 AT5G58610 39.130 437 215 8 3 428 590 986 4.65e-87 286
MS.gene86531.t1 AT5G58610 39.130 437 215 8 3 428 590 986 6.60e-87 286
MS.gene86531.t1 AT5G58610 40.838 382 185 7 3 367 590 947 3.38e-82 271
MS.gene86531.t1 AT5G58610 40.838 382 185 7 3 367 599 956 3.47e-82 271
MS.gene86531.t1 AT5G58610 40.116 344 165 7 3 329 590 909 3.55e-68 233
MS.gene86531.t1 AT5G36740 32.401 429 254 6 3 419 545 949 3.40e-67 232
MS.gene86531.t1 AT5G36740 32.401 429 254 6 3 419 545 949 3.40e-67 232
MS.gene86531.t1 AT3G14980 31.871 433 261 7 3 425 621 1029 1.68e-64 224
MS.gene86531.t1 AT3G14980 31.871 433 261 7 3 425 576 984 3.06e-64 223
MS.gene86531.t1 AT3G14980 31.871 433 261 7 3 425 621 1029 3.90e-64 223
MS.gene86531.t1 AT3G14980 31.871 433 261 7 3 425 621 1029 3.90e-64 223
MS.gene86531.t1 AT3G14980 31.871 433 261 7 3 425 621 1029 3.90e-64 223
MS.gene86531.t1 AT1G05380 33.021 427 255 6 3 419 520 925 6.10e-64 223
MS.gene86531.t1 AT1G05380 33.021 427 255 6 3 419 520 925 6.10e-64 223
MS.gene86531.t1 AT1G05380 33.021 427 255 6 3 419 520 925 6.10e-64 223
MS.gene86531.t1 AT5G36740 31.435 439 255 7 3 419 392 806 1.01e-63 221
MS.gene86531.t1 AT5G63900 36.858 331 190 8 94 421 228 542 3.15e-62 211
MS.gene86531.t1 AT4G14920 31.019 432 261 6 3 422 580 986 4.65e-60 211
MS.gene86531.t1 AT4G14920 31.019 432 261 6 3 422 580 986 4.65e-60 211
MS.gene86531.t1 AT4G14920 31.019 432 261 6 3 422 580 986 4.94e-60 211
MS.gene86531.t1 AT5G36670 30.337 445 256 8 3 419 545 963 8.53e-59 208
MS.gene86531.t1 AT5G36670 30.337 445 256 8 3 419 545 963 8.53e-59 208
MS.gene86531.t1 AT5G36740 32.345 371 215 6 3 361 545 891 4.60e-57 202
MS.gene86531.t1 AT5G36740 32.345 371 215 6 3 361 545 891 4.60e-57 202
MS.gene86531.t1 AT5G36670 32.345 371 215 6 3 361 545 891 4.60e-57 202
MS.gene86531.t1 AT5G36670 29.451 455 257 9 3 419 392 820 4.38e-55 197
MS.gene86531.t1 AT5G36670 29.451 455 257 9 3 419 545 973 9.62e-55 196
MS.gene86531.t1 AT3G14980 30.769 338 200 7 3 330 621 934 7.03e-43 161
MS.gene86531.t1 AT3G53680 28.261 460 241 15 13 417 405 830 3.66e-40 153
MS.gene86531.t1 AT3G53680 28.261 460 241 15 13 417 403 828 3.93e-40 153
MS.gene86531.t1 AT2G37520 25.910 467 244 12 13 417 386 812 4.58e-40 153
MS.gene86531.t1 AT2G36720 25.470 479 251 14 13 423 533 973 5.00e-39 150
MS.gene86531.t1 AT2G36720 25.470 479 251 14 13 423 533 973 5.00e-39 150
MS.gene86531.t1 AT2G27980 24.390 492 252 16 10 422 610 1060 1.03e-31 128

Find 69 sgRNAs with CRISPR-Local

Find 86 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGATTATCTGCAACCTTAAT+TGG 0.181951 5.1:+15143820 None:intergenic
TTCACATTCTTGTTTGCATT+TGG 0.183779 5.1:+15144005 None:intergenic
GTTTGTTCAGTTTGTGGTTT+TGG 0.236361 5.1:-15144150 MS.gene86531:CDS
TGGTTTCTATAATGTGATTT+TGG 0.241530 5.1:-15143605 MS.gene86531:CDS
GTGGCAACTATAAGGATTTA+TGG 0.245404 5.1:-15143559 MS.gene86531:CDS
ATCAACTCTCTTCCTGATAA+AGG 0.280267 5.1:+15143679 None:intergenic
TCACATTCTTGTTTGCATTT+GGG 0.312904 5.1:+15144006 None:intergenic
GCATTCCACCTCCGTTGTTT+AGG 0.320877 5.1:-15144093 MS.gene86531:CDS
GAACGATGGGTATTGTTTCT+TGG 0.326060 5.1:-15144536 None:intergenic
GTTGATAACGAATCAAGTTT+TGG 0.327450 5.1:-15144514 MS.gene86531:CDS
TGATTTCAGTGGCAACTATA+AGG 0.339394 5.1:-15143567 MS.gene86531:CDS
TTATGTCTAAAGAAGTTGTT+AGG 0.347395 5.1:-15143847 MS.gene86531:CDS
AAAGGAGAGTAAGTTGAATA+TGG 0.354999 5.1:-15143743 MS.gene86531:CDS
AATTTAATCACATGGAGAAT+TGG 0.356404 5.1:-15143900 MS.gene86531:CDS
GATAGTGTTTGTTCAGTTTG+TGG 0.378867 5.1:-15144156 MS.gene86531:CDS
CTGGGTTCGCATGATCATAA+AGG 0.384834 5.1:-15144285 MS.gene86531:CDS
GGATTGTCAAAGGGAAGCTC+TGG 0.400874 5.1:-15144304 MS.gene86531:CDS
AACACTTGGACAAGATCATT+TGG 0.403595 5.1:-15143388 MS.gene86531:CDS
TGAAGAGGAGTTTAATCAAA+AGG 0.404213 5.1:-15143761 MS.gene86531:CDS
AAGCACTGAAAAGACCTATA+TGG 0.410177 5.1:-15143282 MS.gene86531:CDS
GGCTCTTGGTGTATTGTATG+AGG 0.423950 5.1:-15143722 MS.gene86531:CDS
ACCATCCACAAGTATCTTGT+TGG 0.424383 5.1:-15144346 MS.gene86531:CDS
GTTGTTAGGAAAACCAATTA+AGG 0.435095 5.1:-15143833 MS.gene86531:CDS
ATTGAACACAAACAAGAATC+TGG 0.437308 5.1:+15143968 None:intergenic
AGTAACAAAGTTGTCAAAAG+AGG 0.441397 5.1:-15144462 MS.gene86531:CDS
ATCGAGTTCCGCTTGGTGAA+TGG 0.464422 5.1:-15144065 MS.gene86531:CDS
CAAGCAATGCAAGGCCATAT+AGG 0.465235 5.1:+15143268 None:intergenic
CCTGATAAAGGATCAAAAGT+TGG 0.477884 5.1:+15143691 None:intergenic
ACAAGTATCTTGTTGGAAGA+TGG 0.480189 5.1:-15144339 MS.gene86531:CDS
TTGTCTTCGATACTGTTCCT+TGG 0.482098 5.1:+15143504 None:intergenic
GGAAGATGGAAGGTCGTTGT+TGG 0.488980 5.1:-15144325 MS.gene86531:CDS
GCTCTTGGTGTATTGTATGA+GGG 0.493509 5.1:-15143721 MS.gene86531:CDS
TGGGTTCGCATGATCATAAA+GGG 0.494630 5.1:-15144284 MS.gene86531:CDS
GAGAAACAGTTAACTCGCTT+AGG 0.495020 5.1:-15143451 MS.gene86531:CDS
AGGAATGTGTCATTTGTTGA+TGG 0.496868 5.1:-15143482 MS.gene86531:CDS
CAGAGGATGCTATGAACACT+TGG 0.500216 5.1:-15143402 MS.gene86531:CDS
GTGATTAAATTCAATTCCTA+CGG 0.512914 5.1:+15143912 None:intergenic
GTCGTTGTTGGATTGTCAAA+GGG 0.517227 5.1:-15144313 MS.gene86531:CDS
TCTTCGATACTGTTCCTTGG+TGG 0.524421 5.1:+15143507 None:intergenic
GGTCGTTGTTGGATTGTCAA+AGG 0.528790 5.1:-15144314 MS.gene86531:CDS
AGTAAGTTGAATATGGCTCT+TGG 0.540429 5.1:-15143736 MS.gene86531:CDS
ATCTTCCAACAAGATACTTG+TGG 0.550941 5.1:+15144341 None:intergenic
GGTCTCAATCGAGTTCCGCT+TGG 0.551276 5.1:-15144072 MS.gene86531:CDS
ATATCACTTTGGATGCGTAA+AGG 0.554258 5.1:-15143935 MS.gene86531:CDS
TGTGAATAGTGTTGATGAAG+AGG 0.557314 5.1:-15143776 MS.gene86531:CDS
ACCGGCTTCGAGTCTCATGC+AGG 0.558836 5.1:-15144384 MS.gene86531:CDS
GTAACAAAGTTGTCAAAAGA+GGG 0.581494 5.1:-15144461 MS.gene86531:CDS
TGAGACCTAAACAACGGAGG+TGG 0.586505 5.1:+15144088 None:intergenic
GATTGTCAAAGGGAAGCTCT+GGG 0.597706 5.1:-15144303 MS.gene86531:CDS
AGGAATTGAATTTAATCACA+TGG 0.598130 5.1:-15143908 MS.gene86531:CDS
TTTCAGTGCTTTGAGACACA+TGG 0.604056 5.1:+15143294 None:intergenic
AGATAATCTAACATGGACAT+TGG 0.607457 5.1:-15143806 MS.gene86531:CDS
CTCCAACATCAAGCAATGCA+AGG 0.609626 5.1:+15143259 None:intergenic
TCCAACAAGATACTTGTGGA+TGG 0.616702 5.1:+15144345 None:intergenic
CAACGGAGGTGGAATGCAGA+AGG 0.622944 5.1:+15144099 None:intergenic
GTATCTTGTTGGAAGATGGA+AGG 0.629827 5.1:-15144335 MS.gene86531:CDS
ATTACTTCTGCATTCCTCAG+AGG 0.633654 5.1:-15143419 MS.gene86531:CDS
ACAAGAATGTGAAGATCACA+AGG 0.637763 5.1:-15143995 MS.gene86531:CDS
AATGGGTGAAGTGATTTCAG+TGG 0.642896 5.1:-15143578 MS.gene86531:CDS
TTTGGATGCGTAAAGGCCGT+AGG 0.643030 5.1:-15143928 MS.gene86531:CDS
ACCTGCATGAGACTCGAAGC+CGG 0.643864 5.1:+15144383 None:intergenic
AAATCACATTATAGAAACCA+CGG 0.660195 5.1:+15143608 None:intergenic
AGGTTGCAGATAATCTAACA+TGG 0.665981 5.1:-15143813 MS.gene86531:CDS
GGACAAAACCATTCACCAAG+CGG 0.675521 5.1:+15144057 None:intergenic
CTCGATTGAGACCTAAACAA+CGG 0.677857 5.1:+15144082 None:intergenic
AAGGAACAGTATCGAAGACA+AGG 0.678537 5.1:-15143502 MS.gene86531:CDS
ACTACCCTGACAGAAAACAT+TGG 0.690433 5.1:+15144482 None:intergenic
AGTGTTCATAGCATCCTCTG+AGG 0.736105 5.1:+15143405 None:intergenic
GATTGAGACCTAAACAACGG+AGG 0.762353 5.1:+15144085 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ACTTTTTTAGAATTTGAGAA+TGG - chr5.1:15144479-15144498 MS.gene86531:CDS 20.0%
! AAATCACATTATAGAAACCA+CGG + chr5.1:15144193-15144212 None:intergenic 25.0%
! AATTTAATCACATGGAGAAT+TGG - chr5.1:15143898-15143917 MS.gene86531:CDS 25.0%
! AGGAATTGAATTTAATCACA+TGG - chr5.1:15143890-15143909 MS.gene86531:CDS 25.0%
! GTGATTAAATTCAATTCCTA+CGG + chr5.1:15143889-15143908 None:intergenic 25.0%
! TTATGTCTAAAGAAGTTGTT+AGG - chr5.1:15143951-15143970 MS.gene86531:CDS 25.0%
!! TGGTTTCTATAATGTGATTT+TGG - chr5.1:15144193-15144212 MS.gene86531:CDS 25.0%
!!! AATGTGATTTTGGAGAAAAT+GGG - chr5.1:15144203-15144222 MS.gene86531:CDS 25.0%
!!! TAATGTGATTTTGGAGAAAA+TGG - chr5.1:15144202-15144221 MS.gene86531:CDS 25.0%
AGATAATCTAACATGGACAT+TGG - chr5.1:15143992-15144011 MS.gene86531:CDS 30.0%
AGATTATCTGCAACCTTAAT+TGG + chr5.1:15143981-15144000 None:intergenic 30.0%
AGTAACAAAGTTGTCAAAAG+AGG - chr5.1:15143336-15143355 MS.gene86531:CDS 30.0%
ATTGAACACAAACAAGAATC+TGG + chr5.1:15143833-15143852 None:intergenic 30.0%
GTAACAAAGTTGTCAAAAGA+GGG - chr5.1:15143337-15143356 MS.gene86531:CDS 30.0%
GTTGTTAGGAAAACCAATTA+AGG - chr5.1:15143965-15143984 MS.gene86531:CDS 30.0%
TGAAGAGGAGTTTAATCAAA+AGG - chr5.1:15144037-15144056 MS.gene86531:CDS 30.0%
TGTGAGCAAAAATATCACTT+TGG - chr5.1:15143852-15143871 MS.gene86531:CDS 30.0%
! AAAGGAGAGTAAGTTGAATA+TGG - chr5.1:15144055-15144074 MS.gene86531:CDS 30.0%
! CATATTTTGCAACAACAAGT+TGG + chr5.1:15143765-15143784 None:intergenic 30.0%
! TCACATTCTTGTTTGCATTT+GGG + chr5.1:15143795-15143814 None:intergenic 30.0%
! TTCACATTCTTGTTTGCATT+TGG + chr5.1:15143796-15143815 None:intergenic 30.0%
!!! GTTGATAACGAATCAAGTTT+TGG - chr5.1:15143284-15143303 MS.gene86531:CDS 30.0%
AACACTTGGACAAGATCATT+TGG - chr5.1:15144410-15144429 MS.gene86531:CDS 35.0%
ACAAGAATGTGAAGATCACA+AGG - chr5.1:15143803-15143822 MS.gene86531:CDS 35.0%
ACAAGTATCTTGTTGGAAGA+TGG - chr5.1:15143459-15143478 MS.gene86531:CDS 35.0%
AGGTTGCAGATAATCTAACA+TGG - chr5.1:15143985-15144004 MS.gene86531:CDS 35.0%
ATATCACTTTGGATGCGTAA+AGG - chr5.1:15143863-15143882 MS.gene86531:CDS 35.0%
ATCAACTCTCTTCCTGATAA+AGG + chr5.1:15144122-15144141 None:intergenic 35.0%
ATCTTCCAACAAGATACTTG+TGG + chr5.1:15143460-15143479 None:intergenic 35.0%
CCTGATAAAGGATCAAAAGT+TGG + chr5.1:15144110-15144129 None:intergenic 35.0%
GATAGTGTTTGTTCAGTTTG+TGG - chr5.1:15143642-15143661 MS.gene86531:CDS 35.0%
GTGGCAACTATAAGGATTTA+TGG - chr5.1:15144239-15144258 MS.gene86531:CDS 35.0%
TGATTTCAGTGGCAACTATA+AGG - chr5.1:15144231-15144250 MS.gene86531:CDS 35.0%
! AGTAAGTTGAATATGGCTCT+TGG - chr5.1:15144062-15144081 MS.gene86531:CDS 35.0%
! CCAACTTTTGATCCTTTATC+AGG - chr5.1:15144107-15144126 MS.gene86531:CDS 35.0%
! GTTTGTTCAGTTTGTGGTTT+TGG - chr5.1:15143648-15143667 MS.gene86531:CDS 35.0%
!! AAGCACTGAAAAGACCTATA+TGG - chr5.1:15144516-15144535 MS.gene86531:CDS 35.0%
!! AGGAATGTGTCATTTGTTGA+TGG - chr5.1:15144316-15144335 MS.gene86531:CDS 35.0%
!! TGTGAATAGTGTTGATGAAG+AGG - chr5.1:15144022-15144041 MS.gene86531:CDS 35.0%
!!! ACGAATCAAGTTTTGGTTTC+AGG - chr5.1:15143291-15143310 MS.gene86531:CDS 35.0%
!!! CAAGATCATTTGGTTTTGCA+AGG - chr5.1:15144420-15144439 MS.gene86531:CDS 35.0%
!!! CGAATCAAGTTTTGGTTTCA+GGG - chr5.1:15143292-15143311 MS.gene86531:CDS 35.0%
!!! CGTGTTACTTTTACTATCAC+CGG - chr5.1:15143396-15143415 MS.gene86531:CDS 35.0%
AATGGGTGAAGTGATTTCAG+TGG - chr5.1:15144220-15144239 MS.gene86531:CDS 40.0%
ACCATCCACAAGTATCTTGT+TGG - chr5.1:15143452-15143471 MS.gene86531:CDS 40.0%
ACTACCCTGACAGAAAACAT+TGG + chr5.1:15143319-15143338 None:intergenic 40.0%
ATTACTTCTGCATTCCTCAG+AGG - chr5.1:15144379-15144398 MS.gene86531:CDS 40.0%
CAAAAACGCTTAAACTTCCG+TGG - chr5.1:15144173-15144192 MS.gene86531:CDS 40.0%
CTCGATTGAGACCTAAACAA+CGG + chr5.1:15143719-15143738 None:intergenic 40.0%
GAGAAACAGTTAACTCGCTT+AGG - chr5.1:15144347-15144366 MS.gene86531:CDS 40.0%
GTATCTTGTTGGAAGATGGA+AGG - chr5.1:15143463-15143482 MS.gene86531:CDS 40.0%
GTCGTTGTTGGATTGTCAAA+GGG - chr5.1:15143485-15143504 MS.gene86531:CDS 40.0%
TCCAACAAGATACTTGTGGA+TGG + chr5.1:15143456-15143475 None:intergenic 40.0%
TGGGTTCGCATGATCATAAA+GGG - chr5.1:15143514-15143533 MS.gene86531:CDS 40.0%
TTGTCTTCGATACTGTTCCT+TGG + chr5.1:15144297-15144316 None:intergenic 40.0%
! AAGGAACAGTATCGAAGACA+AGG - chr5.1:15144296-15144315 MS.gene86531:CDS 40.0%
! AGTTGCAGAGATAGTTTTCG+TGG - chr5.1:15144268-15144287 MS.gene86531:CDS 40.0%
! GCTCTTGGTGTATTGTATGA+GGG - chr5.1:15144077-15144096 MS.gene86531:CDS 40.0%
!! GGTTTTGCAGTGTAGTTTGT+GGG - chr5.1:15143919-15143938 MS.gene86531:CDS 40.0%
!! TGGTTTTGCAGTGTAGTTTG+TGG - chr5.1:15143918-15143937 MS.gene86531:CDS 40.0%
!! TTTCAGTGCTTTGAGACACA+TGG + chr5.1:15144507-15144526 None:intergenic 40.0%
!!! TGTTCAGTTTGTGGTTTTGG+AGG - chr5.1:15143651-15143670 MS.gene86531:CDS 40.0%
AGTGTTCATAGCATCCTCTG+AGG + chr5.1:15144396-15144415 None:intergenic 45.0%
CAAGCAATGCAAGGCCATAT+AGG + chr5.1:15144533-15144552 None:intergenic 45.0%
CAGAGGATGCTATGAACACT+TGG - chr5.1:15144396-15144415 MS.gene86531:CDS 45.0%
CTGGGTTCGCATGATCATAA+AGG - chr5.1:15143513-15143532 MS.gene86531:CDS 45.0%
GATTGAGACCTAAACAACGG+AGG + chr5.1:15143716-15143735 None:intergenic 45.0%
GATTGTCAAAGGGAAGCTCT+GGG - chr5.1:15143495-15143514 MS.gene86531:CDS 45.0%
GGACAAAACCATTCACCAAG+CGG + chr5.1:15143744-15143763 None:intergenic 45.0%
GGTCGTTGTTGGATTGTCAA+AGG - chr5.1:15143484-15143503 MS.gene86531:CDS 45.0%
TCTTCGATACTGTTCCTTGG+TGG + chr5.1:15144294-15144313 None:intergenic 45.0%
! AGGGACCAATGTTTTCTGTC+AGG - chr5.1:15143311-15143330 MS.gene86531:CDS 45.0%
! GGCTCTTGGTGTATTGTATG+AGG - chr5.1:15144076-15144095 MS.gene86531:CDS 45.0%
! GGGACCAATGTTTTCTGTCA+GGG - chr5.1:15143312-15143331 MS.gene86531:CDS 45.0%
!!! TTGTGGTTTTGGAGGTGACT+TGG - chr5.1:15143659-15143678 MS.gene86531:CDS 45.0%
GCATTCCACCTCCGTTGTTT+AGG - chr5.1:15143705-15143724 MS.gene86531:CDS 50.0%
GGAAGATGGAAGGTCGTTGT+TGG - chr5.1:15143473-15143492 MS.gene86531:CDS 50.0%
GGATTGTCAAAGGGAAGCTC+TGG - chr5.1:15143494-15143513 MS.gene86531:CDS 50.0%
TGAGACCTAAACAACGGAGG+TGG + chr5.1:15143713-15143732 None:intergenic 50.0%
TTTGGATGCGTAAAGGCCGT+AGG - chr5.1:15143870-15143889 MS.gene86531:CDS 50.0%
! ATCGAGTTCCGCTTGGTGAA+TGG - chr5.1:15143733-15143752 MS.gene86531:CDS 50.0%
! GATAGTTTTCGTGGCCACCA+AGG - chr5.1:15144277-15144296 MS.gene86531:CDS 50.0%
ACCTGCATGAGACTCGAAGC+CGG + chr5.1:15143418-15143437 None:intergenic 55.0%
CAACGGAGGTGGAATGCAGA+AGG + chr5.1:15143702-15143721 None:intergenic 55.0%
GGTCTCAATCGAGTTCCGCT+TGG - chr5.1:15143726-15143745 MS.gene86531:CDS 55.0%
! ACCGGCTTCGAGTCTCATGC+AGG - chr5.1:15143414-15143433 MS.gene86531:CDS 60.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 15143267 15144553 15143267 ID=MS.gene86531
chr5.1 mRNA 15143267 15144553 15143267 ID=MS.gene86531.t1;Parent=MS.gene86531
chr5.1 exon 15143267 15144553 15143267 ID=MS.gene86531.t1.exon1;Parent=MS.gene86531.t1
chr5.1 CDS 15143267 15144553 15143267 ID=cds.MS.gene86531.t1;Parent=MS.gene86531.t1
Gene Sequence

>MS.gene86531

ATGGGTATTGTTTCTTGGTTGATAACGAATCAAGTTTTGGTTTCAGGGACCAATGTTTTCTGTCAGGGTAGTAACAAAGTTGTCAAAAGAGGGAGTTTGTTTTCTGATGCTATAGTCTGTGATTGTTGTCGTGTTACTTTTACTATCACCGGCTTCGAGTCTCATGCAGGTTGTACAAGACATAGACCATCCACAAGTATCTTGTTGGAAGATGGAAGGTCGTTGTTGGATTGTCAAAGGGAAGCTCTGGGTTCGCATGATCATAAAGGGAATCATTGTGTTGTCAAAGAAAATCAAAAGAAGAATCATTCTATTGTCAAAGAAAATCGAAAGAAGAATCATTGTGCTGTCAAAGAAAAATCTGAAGCAAATAACGATAGTGTTTGTTCAGTTTGTGGTTTTGGAGGTGACTTGGCACTATGCGATCGATGTCCTTCTGCATTCCACCTCCGTTGTTTAGGTCTCAATCGAGTTCCGCTTGGTGAATGGTTTTGTCCAACTTGTTGTTGCAAAATATGCTATCGACCCAAATGCAAACAAGAATGTGAAGATCACAAGGATAACCAGATTCTTGTTTGTGTTCAATGTGAGCAAAAATATCACTTTGGATGCGTAAAGGCCGTAGGAATTGAATTTAATCACATGGAGAATTGGTTTTGCAGTGTAGTTTGTGGGAACATGTTCTTATGTCTAAAGAAGTTGTTAGGAAAACCAATTAAGGTTGCAGATAATCTAACATGGACATTGGTGAAGAATGTGAATAGTGTTGATGAAGAGGAGTTTAATCAAAAGGAGAGTAAGTTGAATATGGCTCTTGGTGTATTGTATGAGGGTTTTAATCCAACTTTTGATCCTTTATCAGGAAGAGAGTTGATTAAAGATGTTGTTTTTAGTAGAGAATCAGAACAAAAACGCTTAAACTTCCGTGGTTTCTATAATGTGATTTTGGAGAAAATGGGTGAAGTGATTTCAGTGGCAACTATAAGGATTTATGGTCAAAAAGTTGCAGAGATAGTTTTCGTGGCCACCAAGGAACAGTATCGAAGACAAGGAATGTGTCATTTGTTGATGGATGAGATTGAGAAACAGTTAACTCGCTTAGGAGTTGAGAAATTACTTCTGCATTCCTCAGAGGATGCTATGAACACTTGGACAAGATCATTTGGTTTTGCAAGGATGAATAGTAAAGATAAGTGTCAGTTTATTGATCATACTTTTTTAGAATTTGAGAATGGTACCATGTGTCTCAAAGCACTGAAAAGACCTATATGGCCTTGCATTGCTTGA
Protein sequence

>MS.gene86531.t1

MGIVSWLITNQVLVSGTNVFCQGSNKVVKRGSLFSDAIVCDCCRVTFTITGFESHAGCTRHRPSTSILLEDGRSLLDCQREALGSHDHKGNHCVVKENQKKNHSIVKENRKKNHCAVKEKSEANNDSVCSVCGFGGDLALCDRCPSAFHLRCLGLNRVPLGEWFCPTCCCKICYRPKCKQECEDHKDNQILVCVQCEQKYHFGCVKAVGIEFNHMENWFCSVVCGNMFLCLKKLLGKPIKVADNLTWTLVKNVNSVDEEEFNQKESKLNMALGVLYEGFNPTFDPLSGRELIKDVVFSRESEQKRLNFRGFYNVILEKMGEVISVATIRIYGQKVAEIVFVATKEQYRRQGMCHLLMDEIEKQLTRLGVEKLLLHSSEDAMNTWTRSFGFARMNSKDKCQFIDHTFLEFENGTMCLKALKRPIWPCIA