Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86658.t1 | XP_003622139.1 | 93.2 | 222 | 12 | 1 | 1 | 222 | 66 | 284 | 2.00E-98 | 368.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86658.t1 | Q9LSD5 | 58.9 | 209 | 63 | 6 | 1 | 203 | 94 | 285 | 1.5e-38 | 161.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86658.t1 | G7L283 | 93.2 | 222 | 12 | 1 | 1 | 222 | 66 | 284 | 1.4e-98 | 368.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene86658.t1 | TF | TCP |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86658.t1 | MTR_7g028160 | 96.396 | 222 | 5 | 1 | 1 | 222 | 66 | 284 | 2.01e-154 | 429 |
| MS.gene86658.t1 | MTR_5g039600 | 57.292 | 96 | 24 | 3 | 1 | 79 | 83 | 178 | 3.31e-25 | 102 |
| MS.gene86658.t1 | MTR_2g006150 | 75.000 | 64 | 10 | 1 | 1 | 58 | 146 | 209 | 1.51e-24 | 100 |
| MS.gene86658.t1 | MTR_2g006150 | 75.000 | 64 | 10 | 1 | 1 | 58 | 146 | 209 | 1.74e-24 | 100 |
| MS.gene86658.t1 | MTR_8g033070 | 70.968 | 62 | 18 | 0 | 1 | 62 | 96 | 157 | 2.24e-24 | 99.0 |
| MS.gene86658.t1 | MTR_4g108370 | 89.362 | 47 | 5 | 0 | 1 | 47 | 113 | 159 | 8.10e-24 | 98.6 |
| MS.gene86658.t1 | MTR_1g101810 | 70.968 | 62 | 18 | 0 | 1 | 62 | 80 | 141 | 1.01e-23 | 97.1 |
| MS.gene86658.t1 | MTR_1g063870 | 85.106 | 47 | 7 | 0 | 1 | 47 | 65 | 111 | 1.51e-23 | 94.0 |
| MS.gene86658.t1 | MTR_1g101810 | 70.968 | 62 | 18 | 0 | 1 | 62 | 110 | 171 | 1.54e-23 | 97.1 |
| MS.gene86658.t1 | MTR_1g038650 | 62.162 | 74 | 28 | 0 | 1 | 74 | 48 | 121 | 3.65e-23 | 93.6 |
| MS.gene86658.t1 | MTR_1g114380 | 83.333 | 48 | 8 | 0 | 1 | 48 | 49 | 96 | 2.30e-22 | 92.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86658.t1 | AT3G27010 | 56.061 | 198 | 64 | 7 | 1 | 192 | 94 | 274 | 2.60e-49 | 164 |
| MS.gene86658.t1 | AT3G27010 | 56.061 | 198 | 64 | 7 | 1 | 192 | 94 | 274 | 2.60e-49 | 164 |
| MS.gene86658.t1 | AT1G58100 | 75.000 | 64 | 10 | 1 | 1 | 58 | 76 | 139 | 2.56e-25 | 102 |
| MS.gene86658.t1 | AT5G41030 | 35.437 | 206 | 69 | 11 | 1 | 204 | 84 | 227 | 1.03e-24 | 98.2 |
| MS.gene86658.t1 | AT2G45680 | 64.706 | 68 | 24 | 0 | 1 | 68 | 91 | 158 | 4.95e-24 | 98.2 |
| MS.gene86658.t1 | AT3G47620 | 91.489 | 47 | 4 | 0 | 1 | 47 | 133 | 179 | 6.16e-24 | 99.4 |
| MS.gene86658.t1 | AT1G69690 | 85.417 | 48 | 7 | 0 | 1 | 48 | 68 | 115 | 6.42e-24 | 97.4 |
| MS.gene86658.t1 | AT1G35560 | 73.438 | 64 | 11 | 1 | 1 | 58 | 71 | 134 | 7.57e-24 | 97.4 |
| MS.gene86658.t1 | AT1G72010 | 87.234 | 47 | 6 | 0 | 1 | 47 | 79 | 125 | 1.93e-23 | 97.1 |
| MS.gene86658.t1 | AT5G51910 | 65.625 | 64 | 22 | 0 | 1 | 64 | 73 | 136 | 2.24e-23 | 95.5 |
| MS.gene86658.t1 | AT5G51910 | 65.625 | 64 | 22 | 0 | 1 | 64 | 73 | 136 | 2.24e-23 | 95.5 |
| MS.gene86658.t1 | AT5G08330 | 72.881 | 59 | 16 | 0 | 1 | 59 | 47 | 105 | 2.56e-23 | 94.4 |
| MS.gene86658.t1 | AT5G23280 | 83.333 | 48 | 8 | 0 | 1 | 48 | 55 | 102 | 1.37e-22 | 92.4 |
| MS.gene86658.t1 | AT2G37000 | 84.091 | 44 | 7 | 0 | 1 | 44 | 59 | 102 | 4.22e-19 | 82.0 |
| MS.gene86658.t1 | AT3G45150 | 58.140 | 43 | 18 | 0 | 1 | 43 | 33 | 75 | 1.08e-11 | 61.6 |
Find 56 sgRNAs with CRISPR-Local
Find 64 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAGATGCCTGGTTTGGAATT+AGG | 0.117190 | 7.4:+73883600 | MS.gene86658:CDS |
| ATGTTAGTTCTACAAGTTTA+TGG | 0.160920 | 7.4:+73883382 | MS.gene86658:CDS |
| GATGGGCCTGGTGGGCTTGA+TGG | 0.187359 | 7.4:-73883341 | None:intergenic |
| CAAGGTTCATCTTTGTCTTC+TGG | 0.222188 | 7.4:+73883282 | MS.gene86658:CDS |
| TATTATTATTCTGCTGATTC+TGG | 0.237820 | 7.4:-73883716 | None:intergenic |
| CATCATCAAGTTGGTGGATA+TGG | 0.242402 | 7.4:+73883408 | MS.gene86658:CDS |
| TATGCCAACAGGAACAAATT+TGG | 0.247795 | 7.4:+73883530 | MS.gene86658:CDS |
| AGTTGAATGATAGGAATAAT+TGG | 0.264011 | 7.4:+73883310 | MS.gene86658:CDS |
| AGATGCCTGGTTTGGAATTA+GGG | 0.264298 | 7.4:+73883601 | MS.gene86658:CDS |
| ATGGGCCTGGTGGGCTTGAT+GGG | 0.294523 | 7.4:-73883340 | None:intergenic |
| GCTTCAGCTTTAGCTTCTTC+TGG | 0.304919 | 7.4:+73883243 | MS.gene86658:CDS |
| CCTGTTGCAGCTATGATTGA+TGG | 0.306906 | 7.4:-73883204 | None:intergenic |
| GAATTGTGAGAATCATCTTT+AGG | 0.322739 | 7.4:-73883741 | None:intergenic |
| TTTCATCAATCATCATCTTC+TGG | 0.335762 | 7.4:+73883432 | MS.gene86658:CDS |
| CTTGATGGGCCTGATGGGCC+TGG | 0.347126 | 7.4:-73883353 | None:intergenic |
| TTTCAACTCACAAGAGAGTT+AGG | 0.358109 | 7.4:+73883132 | MS.gene86658:CDS |
| ATGGGCCTGATGGGCCTGGT+GGG | 0.388474 | 7.4:-73883349 | None:intergenic |
| CTGGTTTGCAGTTGAATGAT+AGG | 0.398808 | 7.4:+73883301 | MS.gene86658:CDS |
| ATGCCAACAGGAACAAATTT+GGG | 0.405289 | 7.4:+73883531 | MS.gene86658:CDS |
| TCAAGCCCACCAGGCCCATC+AGG | 0.414777 | 7.4:+73883344 | MS.gene86658:CDS |
| ATCATAGCTGCAACAGGAAC+AGG | 0.443120 | 7.4:+73883210 | MS.gene86658:CDS |
| ACTCAGATTTATCAGCAGAT+TGG | 0.447988 | 7.4:+73883669 | MS.gene86658:CDS |
| TCTGGATTTGATATGCCAAC+AGG | 0.449552 | 7.4:+73883519 | MS.gene86658:CDS |
| ACTCCCAAATTTGTTCCTGT+TGG | 0.452759 | 7.4:-73883534 | None:intergenic |
| GTTGAATGATAGGAATAATT+GGG | 0.466051 | 7.4:+73883311 | MS.gene86658:CDS |
| AAATCAGCAGATGCCTGGTT+TGG | 0.470684 | 7.4:+73883593 | MS.gene86658:CDS |
| AACTAACATGATGACCTTGA+TGG | 0.471715 | 7.4:-73883368 | None:intergenic |
| ATGACCTTGATGGGCCTGAT+GGG | 0.473600 | 7.4:-73883358 | None:intergenic |
| CCATCAATCATAGCTGCAAC+AGG | 0.479395 | 7.4:+73883204 | MS.gene86658:CDS |
| GATGGGCCTGATGGGCCTGG+TGG | 0.480862 | 7.4:-73883350 | None:intergenic |
| TTGTCACAAGATGGACATAT+TGG | 0.480930 | 7.4:+73883624 | MS.gene86658:CDS |
| GAATTAGGGTTGTCACAAGA+TGG | 0.488895 | 7.4:+73883615 | MS.gene86658:CDS |
| ACTGTTGGTGAAAATAATAG+TGG | 0.505174 | 7.4:+73883471 | MS.gene86658:CDS |
| TGGCCTCATCATCAAGTTGG+TGG | 0.511204 | 7.4:+73883402 | MS.gene86658:CDS |
| GGTGCAAATCAGCAGATGCC+TGG | 0.519917 | 7.4:+73883588 | MS.gene86658:CDS |
| TCAGACCCATCAAGCCCACC+AGG | 0.525810 | 7.4:+73883335 | MS.gene86658:CDS |
| CAGATGGTGAAACAATTCAA+TGG | 0.529264 | 7.4:+73883163 | MS.gene86658:CDS |
| AGATTGGTCAAAATCAAACT+AGG | 0.530078 | 7.4:+73883685 | MS.gene86658:CDS |
| CAGGCCCATCAGGCCCATCA+AGG | 0.530139 | 7.4:+73883354 | MS.gene86658:CDS |
| ATTCTTGCTGCACATAAAGC+TGG | 0.536084 | 7.4:-73883108 | None:intergenic |
| AACAGGAACAAATTTGGGAG+TGG | 0.545963 | 7.4:+73883536 | MS.gene86658:CDS |
| GGTTTAGTAGCTACTACTGT+TGG | 0.551441 | 7.4:+73883456 | MS.gene86658:CDS |
| GATGACCTTGATGGGCCTGA+TGG | 0.557322 | 7.4:-73883359 | None:intergenic |
| ACTAACATGATGACCTTGAT+GGG | 0.557914 | 7.4:-73883367 | None:intergenic |
| CATCAATCATCATCTTCTGG+TGG | 0.564429 | 7.4:+73883435 | MS.gene86658:CDS |
| GGAACAAATTTGGGAGTGGG+AGG | 0.568972 | 7.4:+73883540 | MS.gene86658:CDS |
| GAAGAAGCTAAAGCTGAAGC+TGG | 0.576280 | 7.4:-73883240 | None:intergenic |
| GGTAATACTTTGACACCACA+AGG | 0.588387 | 7.4:+73883264 | MS.gene86658:CDS |
| ACAGGAACAAATTTGGGAGT+GGG | 0.594534 | 7.4:+73883537 | MS.gene86658:CDS |
| GATTGGTCAAAATCAAACTA+GGG | 0.595101 | 7.4:+73883686 | MS.gene86658:CDS |
| GAACAAATTTGGGAGTGGGA+GGG | 0.598189 | 7.4:+73883541 | MS.gene86658:CDS |
| TTATGGCCTCATCATCAAGT+TGG | 0.605767 | 7.4:+73883399 | MS.gene86658:CDS |
| GAGTTAGGTCATAAATCAGA+TGG | 0.610512 | 7.4:+73883147 | MS.gene86658:CDS |
| TATCCACCAACTTGATGATG+AGG | 0.610804 | 7.4:-73883405 | None:intergenic |
| GACAACCCTAATTCCAAACC+AGG | 0.628289 | 7.4:-73883606 | None:intergenic |
| GAAGACAAAGATGAACCTTG+TGG | 0.735014 | 7.4:-73883279 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GAAATTATTTTCAGAAAATT+GGG | + | chr7.4:73883493-73883512 | MS.gene86658:CDS | 15.0% |
| !!! | ATGAGTTTTACATCAATTTT+GGG | + | chr7.4:73883564-73883583 | MS.gene86658:CDS | 20.0% |
| !!! | GGAAATTATTTTCAGAAAAT+TGG | + | chr7.4:73883492-73883511 | MS.gene86658:CDS | 20.0% |
| ! | AGTTGAATGATAGGAATAAT+TGG | + | chr7.4:73883310-73883329 | MS.gene86658:CDS | 25.0% |
| ! | GTTGAATGATAGGAATAATT+GGG | + | chr7.4:73883311-73883330 | MS.gene86658:CDS | 25.0% |
| !! | GATGAGTTTTACATCAATTT+TGG | + | chr7.4:73883563-73883582 | MS.gene86658:CDS | 25.0% |
| !! | TGAGTTTTACATCAATTTTG+GGG | + | chr7.4:73883565-73883584 | MS.gene86658:CDS | 25.0% |
| !!! | ATGTTAGTTCTACAAGTTTA+TGG | + | chr7.4:73883382-73883401 | MS.gene86658:CDS | 25.0% |
| !!! | TATTATTATTCTGCTGATTC+TGG | - | chr7.4:73883719-73883738 | None:intergenic | 25.0% |
| GAATTGTGAGAATCATCTTT+AGG | - | chr7.4:73883744-73883763 | None:intergenic | 30.0% | |
| TTTCATCAATCATCATCTTC+TGG | + | chr7.4:73883432-73883451 | MS.gene86658:CDS | 30.0% | |
| ! | ACTGTTGGTGAAAATAATAG+TGG | + | chr7.4:73883471-73883490 | MS.gene86658:CDS | 30.0% |
| ! | AGATTGGTCAAAATCAAACT+AGG | + | chr7.4:73883685-73883704 | MS.gene86658:CDS | 30.0% |
| ! | AGTTTTACATCAATTTTGGG+GGG | + | chr7.4:73883567-73883586 | MS.gene86658:CDS | 30.0% |
| ! | GAGTTTTACATCAATTTTGG+GGG | + | chr7.4:73883566-73883585 | MS.gene86658:CDS | 30.0% |
| ! | GATTGGTCAAAATCAAACTA+GGG | + | chr7.4:73883686-73883705 | MS.gene86658:CDS | 30.0% |
| ! | TTTCAGAAAATTGGGTTTTC+TGG | + | chr7.4:73883501-73883520 | MS.gene86658:CDS | 30.0% |
| AACTAACATGATGACCTTGA+TGG | - | chr7.4:73883371-73883390 | None:intergenic | 35.0% | |
| ACTAACATGATGACCTTGAT+GGG | - | chr7.4:73883370-73883389 | None:intergenic | 35.0% | |
| ACTCAGATTTATCAGCAGAT+TGG | + | chr7.4:73883669-73883688 | MS.gene86658:CDS | 35.0% | |
| ATGCCAACAGGAACAAATTT+GGG | + | chr7.4:73883531-73883550 | MS.gene86658:CDS | 35.0% | |
| CAGATGGTGAAACAATTCAA+TGG | + | chr7.4:73883163-73883182 | MS.gene86658:CDS | 35.0% | |
| GAGTTAGGTCATAAATCAGA+TGG | + | chr7.4:73883147-73883166 | MS.gene86658:CDS | 35.0% | |
| TATGCCAACAGGAACAAATT+TGG | + | chr7.4:73883530-73883549 | MS.gene86658:CDS | 35.0% | |
| TTGTCACAAGATGGACATAT+TGG | + | chr7.4:73883624-73883643 | MS.gene86658:CDS | 35.0% | |
| TTTCAACTCACAAGAGAGTT+AGG | + | chr7.4:73883132-73883151 | MS.gene86658:CDS | 35.0% | |
| AACAGGAACAAATTTGGGAG+TGG | + | chr7.4:73883536-73883555 | MS.gene86658:CDS | 40.0% | |
| ACAGGAACAAATTTGGGAGT+GGG | + | chr7.4:73883537-73883556 | MS.gene86658:CDS | 40.0% | |
| ATTCTTGCTGCACATAAAGC+TGG | - | chr7.4:73883111-73883130 | None:intergenic | 40.0% | |
| CAAGGTTCATCTTTGTCTTC+TGG | + | chr7.4:73883282-73883301 | MS.gene86658:CDS | 40.0% | |
| CATCAATCATCATCTTCTGG+TGG | + | chr7.4:73883435-73883454 | MS.gene86658:CDS | 40.0% | |
| GAAGACAAAGATGAACCTTG+TGG | - | chr7.4:73883282-73883301 | None:intergenic | 40.0% | |
| GAATTAGGGTTGTCACAAGA+TGG | + | chr7.4:73883615-73883634 | MS.gene86658:CDS | 40.0% | |
| GGTAATACTTTGACACCACA+AGG | + | chr7.4:73883264-73883283 | MS.gene86658:CDS | 40.0% | |
| GGTTTAGTAGCTACTACTGT+TGG | + | chr7.4:73883456-73883475 | MS.gene86658:CDS | 40.0% | |
| TCTGGATTTGATATGCCAAC+AGG | + | chr7.4:73883519-73883538 | MS.gene86658:CDS | 40.0% | |
| TTATGGCCTCATCATCAAGT+TGG | + | chr7.4:73883399-73883418 | MS.gene86658:CDS | 40.0% | |
| ! | ACTCCCAAATTTGTTCCTGT+TGG | - | chr7.4:73883537-73883556 | None:intergenic | 40.0% |
| ! | CTGGTTTGCAGTTGAATGAT+AGG | + | chr7.4:73883301-73883320 | MS.gene86658:CDS | 40.0% |
| !! | AGATGCCTGGTTTGGAATTA+GGG | + | chr7.4:73883601-73883620 | MS.gene86658:CDS | 40.0% |
| !! | CATCATCAAGTTGGTGGATA+TGG | + | chr7.4:73883408-73883427 | MS.gene86658:CDS | 40.0% |
| !! | TATCCACCAACTTGATGATG+AGG | - | chr7.4:73883408-73883427 | None:intergenic | 40.0% |
| AAATCAGCAGATGCCTGGTT+TGG | + | chr7.4:73883593-73883612 | MS.gene86658:CDS | 45.0% | |
| ATCATAGCTGCAACAGGAAC+AGG | + | chr7.4:73883210-73883229 | MS.gene86658:CDS | 45.0% | |
| CCATCAATCATAGCTGCAAC+AGG | + | chr7.4:73883204-73883223 | MS.gene86658:CDS | 45.0% | |
| CCTGTTGCAGCTATGATTGA+TGG | - | chr7.4:73883207-73883226 | None:intergenic | 45.0% | |
| GAACAAATTTGGGAGTGGGA+GGG | + | chr7.4:73883541-73883560 | MS.gene86658:CDS | 45.0% | |
| GAAGAAGCTAAAGCTGAAGC+TGG | - | chr7.4:73883243-73883262 | None:intergenic | 45.0% | |
| GACAACCCTAATTCCAAACC+AGG | - | chr7.4:73883609-73883628 | None:intergenic | 45.0% | |
| ! | GCTTCAGCTTTAGCTTCTTC+TGG | + | chr7.4:73883243-73883262 | MS.gene86658:CDS | 45.0% |
| !! | CAGATGCCTGGTTTGGAATT+AGG | + | chr7.4:73883600-73883619 | MS.gene86658:CDS | 45.0% |
| GGAACAAATTTGGGAGTGGG+AGG | + | chr7.4:73883540-73883559 | MS.gene86658:CDS | 50.0% | |
| ! | ATGACCTTGATGGGCCTGAT+GGG | - | chr7.4:73883361-73883380 | None:intergenic | 50.0% |
| !! | TGGCCTCATCATCAAGTTGG+TGG | + | chr7.4:73883402-73883421 | MS.gene86658:CDS | 50.0% |
| GGTGCAAATCAGCAGATGCC+TGG | + | chr7.4:73883588-73883607 | MS.gene86658:CDS | 55.0% | |
| ! | GATGACCTTGATGGGCCTGA+TGG | - | chr7.4:73883362-73883381 | None:intergenic | 55.0% |
| TCAGACCCATCAAGCCCACC+AGG | + | chr7.4:73883335-73883354 | MS.gene86658:CDS | 60.0% | |
| !! | ATGGGCCTGGTGGGCTTGAT+GGG | - | chr7.4:73883343-73883362 | None:intergenic | 60.0% |
| CAGGCCCATCAGGCCCATCA+AGG | + | chr7.4:73883354-73883373 | MS.gene86658:CDS | 65.0% | |
| TCAAGCCCACCAGGCCCATC+AGG | + | chr7.4:73883344-73883363 | MS.gene86658:CDS | 65.0% | |
| ! | ATGGGCCTGATGGGCCTGGT+GGG | - | chr7.4:73883352-73883371 | None:intergenic | 65.0% |
| ! | CTTGATGGGCCTGATGGGCC+TGG | - | chr7.4:73883356-73883375 | None:intergenic | 65.0% |
| !! | GATGGGCCTGGTGGGCTTGA+TGG | - | chr7.4:73883344-73883363 | None:intergenic | 65.0% |
| ! | GATGGGCCTGATGGGCCTGG+TGG | - | chr7.4:73883353-73883372 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 73883105 | 73883773 | 73883105 | ID=MS.gene86658 |
| chr7.4 | mRNA | 73883105 | 73883773 | 73883105 | ID=MS.gene86658.t1;Parent=MS.gene86658 |
| chr7.4 | exon | 73883105 | 73883773 | 73883105 | ID=MS.gene86658.t1.exon1;Parent=MS.gene86658.t1 |
| chr7.4 | CDS | 73883105 | 73883773 | 73883105 | ID=cds.MS.gene86658.t1;Parent=MS.gene86658.t1 |
| Gene Sequence |
| Protein sequence |
