Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86706.t1 | RHN44861.1 | 81.6 | 304 | 42 | 3 | 1 | 291 | 156 | 458 | 2.00E-125 | 458.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86706.t1 | Q9LYP5 | 62.8 | 43 | 16 | 0 | 1 | 43 | 256 | 298 | 3.3e-07 | 57.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86706.t1 | G7L033 | 81.6 | 304 | 42 | 3 | 1 | 291 | 191 | 493 | 1.5e-125 | 458.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene86706.t1 | TF | GARP-G2-like |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86706.t1 | MTR_7g026400 | 82.566 | 304 | 39 | 3 | 1 | 291 | 191 | 493 | 4.90e-164 | 469 |
| MS.gene86706.t1 | MTR_0450s0040 | 40.449 | 178 | 71 | 4 | 1 | 178 | 203 | 345 | 2.18e-24 | 101 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 54 sgRNAs with CRISPR-Local
Find 67 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAGATTCTATTTCTAATATT+AGG | 0.147775 | 7.4:+74780545 | MS.gene86706:CDS |
| CAATTTCCTTATATACAAAA+AGG | 0.214869 | 7.4:+74780025 | MS.gene86706:CDS |
| GAACTATGAACAATGGATTA+AGG | 0.243060 | 7.4:+74780422 | MS.gene86706:CDS |
| TATGCCAGTGTTTGCAAATA+TGG | 0.272397 | 7.4:-74779938 | None:intergenic |
| AGATTCTATTTCTAATATTA+GGG | 0.274157 | 7.4:+74780546 | MS.gene86706:CDS |
| AAATGTAGCAAGTCACTTAC+AGG | 0.277878 | 7.4:+74779596 | MS.gene86706:CDS |
| CAACATGATGTGTTGGAAAT+TGG | 0.289454 | 7.4:-74780000 | None:intergenic |
| TTCGGAATTCAGCTGAATAA+TGG | 0.313022 | 7.4:+74780379 | MS.gene86706:CDS |
| GATATTAGAAACCAAAATTA+TGG | 0.315005 | 7.4:+74780295 | MS.gene86706:CDS |
| AGGGCTGGCTCTTCAAGATT+TGG | 0.319081 | 7.4:+74780045 | MS.gene86706:CDS |
| TTCAAGATTTGGAACAAGTT+AGG | 0.330501 | 7.4:+74780056 | MS.gene86706:CDS |
| AACTATGAACAATGGATTAA+GGG | 0.373260 | 7.4:+74780423 | MS.gene86706:CDS |
| GGTTTGGTTCAAGGAGGGTT+TGG | 0.385385 | 7.4:+74780520 | MS.gene86706:CDS |
| GGAGCAGGAATGGTTGGAAT+TGG | 0.414917 | 7.4:+74780400 | MS.gene86706:CDS |
| TAGAGCTGAACAAGGATTGA+TGG | 0.418771 | 7.4:+74780225 | MS.gene86706:CDS |
| CTGCGAGATGTTGCAGAGAA+AGG | 0.423104 | 7.4:+74779797 | MS.gene86706:CDS |
| GTGTTGGAAATTGGTTGAAA+TGG | 0.423793 | 7.4:-74779991 | None:intergenic |
| GTAAGAAATAACAATCCTTT+CGG | 0.436356 | 7.4:+74780361 | MS.gene86706:CDS |
| AGCTATGATCAATACAGGTT+TGG | 0.444597 | 7.4:+74780124 | MS.gene86706:CDS |
| TAGTGCAAATAACAATGAAA+CGG | 0.469674 | 7.4:+74780450 | MS.gene86706:CDS |
| ACATCAGAAGTAAATTTATG+AGG | 0.469959 | 7.4:-74780340 | None:intergenic |
| CTTCTTCAAGTTTCTTAACA+AGG | 0.470322 | 7.4:+74780257 | MS.gene86706:CDS |
| ATAACAGCTATGATCAATAC+AGG | 0.478599 | 7.4:+74780119 | MS.gene86706:CDS |
| AGTGCAAATAACAATGAAAC+GGG | 0.482006 | 7.4:+74780451 | MS.gene86706:CDS |
| GATTTGGAACAAGTTAGGCT+TGG | 0.484973 | 7.4:+74780061 | MS.gene86706:CDS |
| TCTAATATTAGGGGAGGGAG+TGG | 0.503861 | 7.4:+74780556 | MS.gene86706:CDS |
| GTTGCAGAGAAAGGAATGGT+TGG | 0.508048 | 7.4:+74779806 | MS.gene86706:CDS |
| TAGGCTTGGAAAATCAAAGT+TGG | 0.508790 | 7.4:+74780075 | MS.gene86706:CDS |
| CTCACAGAGCTCTAAGATCA+AGG | 0.512314 | 7.4:+74779837 | MS.gene86706:CDS |
| AGGATCAGTTAATATAATGA+AGG | 0.528442 | 7.4:+74780594 | MS.gene86706:CDS |
| AATAATGGAGCAGGAATGGT+TGG | 0.529804 | 7.4:+74780394 | MS.gene86706:CDS |
| TTCTTCAAGTTTCTTAACAA+GGG | 0.536372 | 7.4:+74780258 | MS.gene86706:CDS |
| GCTGAATAATGGAGCAGGAA+TGG | 0.538668 | 7.4:+74780390 | MS.gene86706:CDS |
| TTTCCATCTAATTTATATCG+AGG | 0.553052 | 7.4:-74780642 | None:intergenic |
| TCTATTTCTAATATTAGGGG+AGG | 0.557220 | 7.4:+74780550 | MS.gene86706:CDS |
| AATTTGGTTTGGTTCAAGGA+GGG | 0.563542 | 7.4:+74780515 | MS.gene86706:CDS |
| ATTCAGCTGAATAATGGAGC+AGG | 0.566994 | 7.4:+74780385 | MS.gene86706:CDS |
| AGTGGAAGAGAGTTTCTAGC+AGG | 0.567708 | 7.4:+74780574 | MS.gene86706:CDS |
| GCAGAGAAAGGAATGGTTGG+AGG | 0.578705 | 7.4:+74779809 | MS.gene86706:CDS |
| ACTTCCATATTTGCAAACAC+TGG | 0.582579 | 7.4:+74779934 | MS.gene86706:CDS |
| CTATTTCTAATATTAGGGGA+GGG | 0.586359 | 7.4:+74780551 | MS.gene86706:CDS |
| AAATTTGGTTTGGTTCAAGG+AGG | 0.591953 | 7.4:+74780514 | MS.gene86706:CDS |
| GGAATTGGAACTATGAACAA+TGG | 0.591999 | 7.4:+74780415 | MS.gene86706:CDS |
| AATTGGCCAACATGATGTGT+TGG | 0.593925 | 7.4:-74780007 | None:intergenic |
| GATTCTATTTCTAATATTAG+GGG | 0.598018 | 7.4:+74780547 | MS.gene86706:CDS |
| GTTATTCTCTGCAACAACAT+CGG | 0.600928 | 7.4:-74780101 | None:intergenic |
| TCTATATGATGAAAAGCTAG+TGG | 0.601942 | 7.4:-74780206 | None:intergenic |
| TCATCATATAGAGCTGAACA+AGG | 0.608032 | 7.4:+74780217 | MS.gene86706:CDS |
| ATTTCTCTTATCATAGACAT+CGG | 0.611317 | 7.4:-74779875 | None:intergenic |
| GTTTGCAAATATGGAAGTGT+TGG | 0.612895 | 7.4:-74779929 | None:intergenic |
| CAATTTCCAACACATCATGT+TGG | 0.618695 | 7.4:+74780001 | MS.gene86706:CDS |
| TTATTCAGCTGAATTCCGAA+AGG | 0.621035 | 7.4:-74780376 | None:intergenic |
| ACACTGGCATATCAAACAAG+AGG | 0.654807 | 7.4:+74779950 | MS.gene86706:CDS |
| AGATGTTGCAGAGAAAGGAA+TGG | 0.689031 | 7.4:+74779802 | MS.gene86706:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATGATGATATTATAATATTA+TGG | - | chr7.4:74779651-74779670 | None:intergenic | 10.0% |
| !! | AATTTCCTTATATACAAAAA+GGG | + | chr7.4:74780026-74780045 | MS.gene86706:CDS | 15.0% |
| !!! | AGATTCTATTTCTAATATTA+GGG | + | chr7.4:74780546-74780565 | MS.gene86706:CDS | 15.0% |
| !!! | TAGATTCTATTTCTAATATT+AGG | + | chr7.4:74780545-74780564 | MS.gene86706:CDS | 15.0% |
| !! | CAATTTCCTTATATACAAAA+AGG | + | chr7.4:74780025-74780044 | MS.gene86706:CDS | 20.0% |
| !! | GATATTAGAAACCAAAATTA+TGG | + | chr7.4:74780295-74780314 | MS.gene86706:CDS | 20.0% |
| !!! | CTTTTTGTATATAAGGAAAT+TGG | - | chr7.4:74780027-74780046 | None:intergenic | 20.0% |
| !!! | GATTCTATTTCTAATATTAG+GGG | + | chr7.4:74780547-74780566 | MS.gene86706:CDS | 20.0% |
| !!! | TCTTTTGTGAATGAAAAATT+TGG | + | chr7.4:74780499-74780518 | MS.gene86706:CDS | 20.0% |
| ! | ACATCAGAAGTAAATTTATG+AGG | - | chr7.4:74780343-74780362 | None:intergenic | 25.0% |
| ! | AGGATCAGTTAATATAATGA+AGG | + | chr7.4:74780594-74780613 | MS.gene86706:CDS | 25.0% |
| ! | ATTTCTCTTATCATAGACAT+CGG | - | chr7.4:74779878-74779897 | None:intergenic | 25.0% |
| ! | GTAAGAAATAACAATCCTTT+CGG | + | chr7.4:74780361-74780380 | MS.gene86706:CDS | 25.0% |
| ! | TAGTGCAAATAACAATGAAA+CGG | + | chr7.4:74780450-74780469 | MS.gene86706:CDS | 25.0% |
| ! | TGTGAATGAAAAATTTGGTT+TGG | + | chr7.4:74780504-74780523 | MS.gene86706:CDS | 25.0% |
| !! | TGCTTTTAACACCATAATTT+TGG | - | chr7.4:74780309-74780328 | None:intergenic | 25.0% |
| !! | TTCTTCAAGTTTCTTAACAA+GGG | + | chr7.4:74780258-74780277 | MS.gene86706:CDS | 25.0% |
| !! | TTTTTGCTTGAAAAATTGGA+AGG | - | chr7.4:74780165-74780184 | None:intergenic | 25.0% |
| !!! | AACTATGAACAATGGATTAA+GGG | + | chr7.4:74780423-74780442 | MS.gene86706:CDS | 25.0% |
| !!! | GAGTTTTTTGCTTGAAAAAT+TGG | - | chr7.4:74780169-74780188 | None:intergenic | 25.0% |
| AGTGCAAATAACAATGAAAC+GGG | + | chr7.4:74780451-74780470 | MS.gene86706:CDS | 30.0% | |
| ATAACAGCTATGATCAATAC+AGG | + | chr7.4:74780119-74780138 | MS.gene86706:CDS | 30.0% | |
| GCAAAAAACTCATTCAATGA+TGG | + | chr7.4:74780178-74780197 | MS.gene86706:CDS | 30.0% | |
| TCTATATGATGAAAAGCTAG+TGG | - | chr7.4:74780209-74780228 | None:intergenic | 30.0% | |
| ! | CTTCTTCAAGTTTCTTAACA+AGG | + | chr7.4:74780257-74780276 | MS.gene86706:CDS | 30.0% |
| ! | TTCAAGATTTGGAACAAGTT+AGG | + | chr7.4:74780056-74780075 | MS.gene86706:CDS | 30.0% |
| !! | CTATTTCTAATATTAGGGGA+GGG | + | chr7.4:74780551-74780570 | MS.gene86706:CDS | 30.0% |
| !! | GAAAAATTTGGTTTGGTTCA+AGG | + | chr7.4:74780511-74780530 | MS.gene86706:CDS | 30.0% |
| !! | GAACTATGAACAATGGATTA+AGG | + | chr7.4:74780422-74780441 | MS.gene86706:CDS | 30.0% |
| !! | TCTATTTCTAATATTAGGGG+AGG | + | chr7.4:74780550-74780569 | MS.gene86706:CDS | 30.0% |
| AAATGTAGCAAGTCACTTAC+AGG | + | chr7.4:74779596-74779615 | MS.gene86706:CDS | 35.0% | |
| ACTTCCATATTTGCAAACAC+TGG | + | chr7.4:74779934-74779953 | MS.gene86706:CDS | 35.0% | |
| AGCTATGATCAATACAGGTT+TGG | + | chr7.4:74780124-74780143 | MS.gene86706:CDS | 35.0% | |
| CAACATGATGTGTTGGAAAT+TGG | - | chr7.4:74780003-74780022 | None:intergenic | 35.0% | |
| CAATTTCCAACACATCATGT+TGG | + | chr7.4:74780001-74780020 | MS.gene86706:CDS | 35.0% | |
| GGAATTGGAACTATGAACAA+TGG | + | chr7.4:74780415-74780434 | MS.gene86706:CDS | 35.0% | |
| GTGTTGGAAATTGGTTGAAA+TGG | - | chr7.4:74779994-74780013 | None:intergenic | 35.0% | |
| GTTATTCTCTGCAACAACAT+CGG | - | chr7.4:74780104-74780123 | None:intergenic | 35.0% | |
| TAGGCTTGGAAAATCAAAGT+TGG | + | chr7.4:74780075-74780094 | MS.gene86706:CDS | 35.0% | |
| TATGCCAGTGTTTGCAAATA+TGG | - | chr7.4:74779941-74779960 | None:intergenic | 35.0% | |
| TCATCATATAGAGCTGAACA+AGG | + | chr7.4:74780217-74780236 | MS.gene86706:CDS | 35.0% | |
| TCCTTATATACAAAAAGGGC+TGG | + | chr7.4:74780030-74780049 | MS.gene86706:CDS | 35.0% | |
| TTATTCAGCTGAATTCCGAA+AGG | - | chr7.4:74780379-74780398 | None:intergenic | 35.0% | |
| TTCGGAATTCAGCTGAATAA+TGG | + | chr7.4:74780379-74780398 | MS.gene86706:CDS | 35.0% | |
| ! | GTTTGCAAATATGGAAGTGT+TGG | - | chr7.4:74779932-74779951 | None:intergenic | 35.0% |
| !! | AAATTTGGTTTGGTTCAAGG+AGG | + | chr7.4:74780514-74780533 | MS.gene86706:CDS | 35.0% |
| !! | AATTTGGTTTGGTTCAAGGA+GGG | + | chr7.4:74780515-74780534 | MS.gene86706:CDS | 35.0% |
| AATTGGCCAACATGATGTGT+TGG | - | chr7.4:74780010-74780029 | None:intergenic | 40.0% | |
| ACACTGGCATATCAAACAAG+AGG | + | chr7.4:74779950-74779969 | MS.gene86706:CDS | 40.0% | |
| AGATGTTGCAGAGAAAGGAA+TGG | + | chr7.4:74779802-74779821 | MS.gene86706:CDS | 40.0% | |
| TAGAGCTGAACAAGGATTGA+TGG | + | chr7.4:74780225-74780244 | MS.gene86706:CDS | 40.0% | |
| ! | AATAATGGAGCAGGAATGGT+TGG | + | chr7.4:74780394-74780413 | MS.gene86706:CDS | 40.0% |
| ! | ACATTTTCTCTGGTCACGTT+AGG | - | chr7.4:74779582-74779601 | None:intergenic | 40.0% |
| ! | ATTCAGCTGAATAATGGAGC+AGG | + | chr7.4:74780385-74780404 | MS.gene86706:CDS | 40.0% |
| ! | GATTTGGAACAAGTTAGGCT+TGG | + | chr7.4:74780061-74780080 | MS.gene86706:CDS | 40.0% |
| ! | GCCAGCCCTTTTTGTATATA+AGG | - | chr7.4:74780034-74780053 | None:intergenic | 40.0% |
| !!! | GTGACTTGCTACATTTTCTC+TGG | - | chr7.4:74779592-74779611 | None:intergenic | 40.0% |
| GTTGCAGAGAAAGGAATGGT+TGG | + | chr7.4:74779806-74779825 | MS.gene86706:CDS | 45.0% | |
| TCTAATATTAGGGGAGGGAG+TGG | + | chr7.4:74780556-74780575 | MS.gene86706:CDS | 45.0% | |
| ! | GCTGAATAATGGAGCAGGAA+TGG | + | chr7.4:74780390-74780409 | MS.gene86706:CDS | 45.0% |
| !! | AGTGGAAGAGAGTTTCTAGC+AGG | + | chr7.4:74780574-74780593 | MS.gene86706:CDS | 45.0% |
| !! | CTCACAGAGCTCTAAGATCA+AGG | + | chr7.4:74779837-74779856 | MS.gene86706:CDS | 45.0% |
| CTGCGAGATGTTGCAGAGAA+AGG | + | chr7.4:74779797-74779816 | MS.gene86706:CDS | 50.0% | |
| GCAGAGAAAGGAATGGTTGG+AGG | + | chr7.4:74779809-74779828 | MS.gene86706:CDS | 50.0% | |
| GGAGCAGGAATGGTTGGAAT+TGG | + | chr7.4:74780400-74780419 | MS.gene86706:CDS | 50.0% | |
| ! | GGTTTGGTTCAAGGAGGGTT+TGG | + | chr7.4:74780520-74780539 | MS.gene86706:CDS | 50.0% |
| !! | AGGGCTGGCTCTTCAAGATT+TGG | + | chr7.4:74780045-74780064 | MS.gene86706:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 74779570 | 74780657 | 74779570 | ID=MS.gene86706 |
| chr7.4 | mRNA | 74779570 | 74780657 | 74779570 | ID=MS.gene86706.t1;Parent=MS.gene86706 |
| chr7.4 | exon | 74779570 | 74779617 | 74779570 | ID=MS.gene86706.t1.exon1;Parent=MS.gene86706.t1 |
| chr7.4 | CDS | 74779570 | 74779617 | 74779570 | ID=cds.MS.gene86706.t1;Parent=MS.gene86706.t1 |
| chr7.4 | exon | 74779782 | 74780657 | 74779782 | ID=MS.gene86706.t1.exon2;Parent=MS.gene86706.t1 |
| chr7.4 | CDS | 74779782 | 74780657 | 74779782 | ID=cds.MS.gene86706.t1;Parent=MS.gene86706.t1 |
| Gene Sequence |
| Protein sequence |
