AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene86729

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene86729.t1 MTR_3g029590 97.275 734 15 4 1 732 1 731 0.0 1457
MS.gene86729.t1 MTR_7g092070 64.206 718 218 17 33 731 33 730 0.0 830
MS.gene86729.t1 MTR_0728s0020 58.039 622 227 12 29 642 13 608 0.0 635
MS.gene86729.t1 MTR_1g050438 58.049 615 224 12 33 639 1 589 0.0 629
MS.gene86729.t1 MTR_3g435590 63.783 497 101 4 1 497 1 418 0.0 604
MS.gene86729.t1 MTR_3g435590 51.087 92 43 2 591 682 379 468 6.03e-18 87.8
MS.gene86729.t1 MTR_3g435680 62.408 407 89 9 95 497 5 351 2.78e-152 448
MS.gene86729.t1 MTR_5g082830 40.725 717 327 22 45 729 28 678 1.21e-144 439
MS.gene86729.t1 MTR_5g071070 39.208 732 333 19 45 729 33 699 3.17e-140 428
MS.gene86729.t1 MTR_5g071070 39.003 682 304 19 95 729 1 617 1.81e-125 387
MS.gene86729.t1 MTR_1g075890 38.268 635 277 17 44 668 30 559 9.94e-121 376
MS.gene86729.t1 MTR_3g070880 34.342 661 244 17 45 691 8 492 3.83e-104 328
MS.gene86729.t1 MTR_3g464260 42.651 347 126 8 52 392 30 309 1.40e-76 256
MS.gene86729.t1 MTR_3g464280 34.752 423 164 11 49 457 19 343 6.50e-59 207
MS.gene86729.t1 MTR_6g090380 72.897 107 29 0 262 368 99 205 1.76e-55 195
MS.gene86729.t1 MTR_8g102100 52.410 166 68 2 262 420 77 238 5.55e-52 185
MS.gene86729.t1 MTR_2g090605 67.568 111 36 0 266 376 77 187 8.33e-52 186
MS.gene86729.t1 MTR_3g103960 62.281 114 42 1 256 368 43 156 1.24e-48 173
MS.gene86729.t1 MTR_4g078660 61.682 107 41 0 262 368 56 162 2.56e-47 170
MS.gene86729.t1 MTR_3g464270 32.483 431 196 15 265 682 8 356 2.98e-46 169
MS.gene86729.t1 MTR_3g464290 31.602 462 211 16 245 687 79 454 2.70e-40 155
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene86729.t1 AT2G41900 59.157 759 235 24 1 731 1 712 0.0 771
MS.gene86729.t1 AT5G12850 52.727 715 268 23 41 729 36 706 0.0 667
MS.gene86729.t1 AT5G58620 41.515 660 283 18 44 685 11 585 2.52e-139 423
MS.gene86729.t1 AT2G40140 37.639 720 295 23 26 729 10 591 9.17e-125 385
MS.gene86729.t1 AT2G40140 37.639 720 295 23 26 729 10 591 9.17e-125 385
MS.gene86729.t1 AT3G55980 37.867 647 268 17 47 686 30 549 1.05e-115 361
MS.gene86729.t1 AT3G55980 37.867 647 268 17 47 686 97 616 1.18e-114 360
MS.gene86729.t1 AT2G19810 68.033 122 37 1 251 370 61 182 2.60e-55 194
MS.gene86729.t1 AT4G29190 53.939 165 70 1 255 413 68 232 8.80e-55 192
MS.gene86729.t1 AT2G25900 69.725 109 33 0 262 370 86 194 1.21e-54 191
MS.gene86729.t1 AT2G25900 69.725 109 33 0 262 370 86 194 1.21e-54 191
MS.gene86729.t1 AT5G07500 61.983 121 46 0 248 368 34 154 6.09e-53 183
MS.gene86729.t1 AT1G03790 54.601 163 66 3 223 381 43 201 2.97e-52 186
MS.gene86729.t1 AT5G44260 61.719 128 44 2 244 370 42 165 8.92e-49 176

Find 191 sgRNAs with CRISPR-Local

Find 220 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTTTATCATCTGCTGAAAA+TGG 0.161392 3.4:-22808504 MS.gene86729:CDS
ACAGCTGAGTCGGTAAATTT+TGG 0.186788 3.4:+22808459 None:intergenic
CGGCATTGATCACTCTGTTT+TGG 0.190245 3.4:-22807020 MS.gene86729:CDS
CAGCTGTAGTGAAACTTTCT+AGG 0.248659 3.4:+22808249 None:intergenic
TAGGAAGCAGCCACCATTAA+AGG 0.251482 3.4:+22808876 None:intergenic
TGAGCACCGAACTCCTTTAA+TGG 0.259664 3.4:-22808889 MS.gene86729:CDS
CTAGTTTGATTAATGCTTCC+TGG 0.298175 3.4:+22807835 None:intergenic
CTTCTAGGCGACATCCTTCC+AGG 0.313255 3.4:+22807421 None:intergenic
AACCAGGACTCGATGAAATA+AGG 0.317452 3.4:+22808535 None:intergenic
CACTTAGACCAGGAGTTAAC+AGG 0.326627 3.4:+22807262 None:intergenic
CATACTGAGCCTGGTGGAAT+AGG 0.327090 3.4:+22807455 None:intergenic
TTCGGTGCTCAAGAACAATT+TGG 0.328148 3.4:+22808901 None:intergenic
CCCTCTCTCCAATGAGTTCT+CGG 0.331992 3.4:-22807390 MS.gene86729:CDS
TTATTAATGAAGTTGGCTTC+TGG 0.334337 3.4:-22808947 MS.gene86729:CDS
TGTGTTTGACCGAAGTCATT+TGG 0.347057 3.4:+22807199 None:intergenic
GCAAGCTTCTCTTTCTTCTC+AGG 0.349206 3.4:+22807094 None:intergenic
ATGCCGTCCCCTTGAGGGTT+GGG 0.354109 3.4:+22807037 None:intergenic
ATCAATTCGGTGGATGCTAA+TGG 0.355260 3.4:-22808693 MS.gene86729:CDS
TACAGCGGACGCAGCTCTTC+AGG 0.358359 3.4:+22808063 None:intergenic
AAAGAGAAGCTTGCAGGTTC+CGG 0.358491 3.4:-22807085 MS.gene86729:CDS
GAGAGGGCTTCCATACTTCT+AGG 0.360535 3.4:+22807406 None:intergenic
GAGAAGAAAGAGAAGCTTGC+AGG 0.368507 3.4:-22807091 MS.gene86729:CDS
ATACTGAGCCTGGTGGAATA+GGG 0.368901 3.4:+22807456 None:intergenic
TTCTCTTTCTTCTCAGGTGT+GGG 0.372072 3.4:+22807100 None:intergenic
GATATGAGAGTATAAGCTTT+AGG 0.383249 3.4:+22808838 None:intergenic
TTTCAAGCCTTCGAGCTTAT+GGG 0.383519 3.4:+22808629 None:intergenic
AAGGATGTCGCCTAGAAGTA+TGG 0.388115 3.4:-22807416 MS.gene86729:CDS
AATAGATCATCAAGATTTGA+TGG 0.389042 3.4:+22807640 None:intergenic
AACAATCCTGTCTCAGCTGT+TGG 0.394825 3.4:-22807289 MS.gene86729:CDS
TTAATGGTGGCTGCTTCCTA+TGG 0.398871 3.4:-22808873 MS.gene86729:CDS
AGCTTTGAAGCTGTTGAAAC+TGG 0.398962 3.4:+22807530 None:intergenic
GAAATCTTCAAAATCATTGT+TGG 0.399103 3.4:+22808998 None:intergenic
AGCAGTAGAAGCAGCTGATC+GGG 0.402586 3.4:+22808011 None:intergenic
CCATTTGCAGATGGGGATAG+AGG 0.407663 3.4:+22807901 None:intergenic
AGGGGATGCTCTACGTTTGT+AGG 0.408542 3.4:+22807475 None:intergenic
GCTCTTCTCCATTGTTTCCA+TGG 0.413958 3.4:+22807158 None:intergenic
GAGATTGATGATGGTGAACC+AGG 0.414210 3.4:+22807952 None:intergenic
CTTCTCTTTCTTCTCAGGTG+TGG 0.414946 3.4:+22807099 None:intergenic
GCTTCACTTATTAATGAAGT+TGG 0.420259 3.4:-22808954 MS.gene86729:CDS
ATTCCGCATGTATTCATTCA+AGG 0.421704 3.4:-22808355 MS.gene86729:CDS
TCAGGAAGAGATGGGTCAAC+TGG 0.426785 3.4:+22808408 None:intergenic
CAGTATGGTCTCTCTTCTCC+TGG 0.430222 3.4:-22807439 MS.gene86729:CDS
AGCCTTGTCCGATACTGAGC+TGG 0.430472 3.4:+22808138 None:intergenic
GCTATTTCAGTCGGTCGATC+TGG 0.432976 3.4:-22807682 MS.gene86729:CDS
ACAATCCTGTCTCAGCTGTT+GGG 0.435810 3.4:-22807288 MS.gene86729:CDS
AATATGTTGCAAGACTTTGA+TGG 0.442806 3.4:-22807757 MS.gene86729:CDS
TGTTTCTCACAGCCCCGTCC+TGG 0.446692 3.4:-22807706 MS.gene86729:CDS
TTCTCTCCAGGATGCACAAA+AGG 0.447031 3.4:+22808288 None:intergenic
CTTCTGGTATGTCCGTCAAA+AGG 0.447129 3.4:-22808931 MS.gene86729:CDS
CTCCTATTGGAAAAGCTGAT+TGG 0.449350 3.4:-22807231 MS.gene86729:CDS
TCAATTCGGTGGATGCTAAT+GGG 0.451393 3.4:-22808692 MS.gene86729:CDS
AAATGAAGAGCTGGCACATT+GGG 0.453972 3.4:+22807859 None:intergenic
AGCTTATGGGGAACAACAAT+AGG 0.455127 3.4:+22808642 None:intergenic
AGACATGACATTTGTGTTGA+TGG 0.455769 3.4:+22807498 None:intergenic
AAAAGGGGCTTGCAGACGTT+CGG 0.457342 3.4:-22808205 MS.gene86729:CDS
TTGGGTCCCCTGTTAACTCC+TGG 0.457724 3.4:-22807270 MS.gene86729:CDS
AGACAGCAGATTTGTGTGTT+GGG 0.461702 3.4:+22807557 None:intergenic
TTGGCTTGAGCAACTGCAGC+TGG 0.464022 3.4:-22806993 MS.gene86729:CDS
CTGTGCTGCCTCAAGTGGTT+CGG 0.464047 3.4:-22808763 MS.gene86729:CDS
CTCCTTCGAGCATTCTCTCC+AGG 0.464846 3.4:+22808276 None:intergenic
TCCCTTATTTCATCGAGTCC+TGG 0.465549 3.4:-22808537 MS.gene86729:CDS
AAGGCTCTGCAAAGACGGTA+TGG 0.467673 3.4:-22808121 MS.gene86729:CDS
TCCTCTTCATCTGCGGCTGA+AGG 0.470214 3.4:-22809104 MS.gene86729:CDS
CGTCCGCTGTATGTGTCCAC+TGG 0.470219 3.4:-22808051 MS.gene86729:CDS
GACTGAAATAGCACCAGGAC+GGG 0.471789 3.4:+22807693 None:intergenic
CTTTATCATCTGCTGAAAAT+GGG 0.474367 3.4:-22808503 MS.gene86729:CDS
GAAAATACAGAAGCCGCGGC+AGG 0.477087 3.4:+22807580 None:intergenic
TAATGGTGGCTGCTTCCTAT+GGG 0.478863 3.4:-22808872 MS.gene86729:CDS
GATCAATGCCGTCCCCTTGA+GGG 0.479594 3.4:+22807032 None:intergenic
ATTTGTGTTGATGGGTGATA+AGG 0.483158 3.4:+22807507 None:intergenic
CTCATGGGGTATTTGAGTGC+TGG 0.484149 3.4:-22808167 MS.gene86729:CDS
TTCTGGTATGTCCGTCAAAA+GGG 0.484629 3.4:-22808930 MS.gene86729:CDS
CCTCTATCCCCATCTGCAAA+TGG 0.492687 3.4:-22807901 MS.gene86729:CDS
GCATTATTGCCATTTGCAGA+TGG 0.493990 3.4:+22807892 None:intergenic
CATTATTGCCATTTGCAGAT+GGG 0.495177 3.4:+22807893 None:intergenic
ACCAGGACTCGATGAAATAA+GGG 0.495790 3.4:+22808536 None:intergenic
ATGATGGTGAACCAGGGAAA+AGG 0.496915 3.4:+22807959 None:intergenic
GGAAGCATGTTCGAGTAAGC+TGG 0.497010 3.4:+22809019 None:intergenic
CTATTCCACCAGGCTCAGTA+TGG 0.498689 3.4:-22807454 MS.gene86729:CDS
TGTTAACTCCTGGTCTAAGT+GGG 0.498891 3.4:-22807260 MS.gene86729:CDS
GGAACAACAATAACATCGAC+AGG 0.499757 3.4:+22808663 None:intergenic
CTTCACTGTGCTGCCTCAAG+TGG 0.502797 3.4:-22808768 MS.gene86729:CDS
AGAAGAGCCTGATGTAGGTT+GGG 0.503303 3.4:-22807143 MS.gene86729:CDS
TTTGGAGCCACAGGAGAGGA+TGG 0.503694 3.4:+22808477 None:intergenic
GCTGACAACCGAGAACTCAT+TGG 0.504299 3.4:+22807382 None:intergenic
AGCTGGTGCTGATATCAATT+CGG 0.504695 3.4:-22808706 MS.gene86729:CDS
CTCGTGCATACTCTCATGAT+TGG 0.506954 3.4:-22808320 MS.gene86729:CDS
CTGCAAATGGCAATAATGCT+TGG 0.507435 3.4:-22807888 MS.gene86729:CDS
AAGTGGGGATCATCTCCTAT+TGG 0.507586 3.4:-22807244 MS.gene86729:CDS
CTTCCTGGTAAATGAAGAGC+TGG 0.509476 3.4:+22807850 None:intergenic
CTGTTAACTCCTGGTCTAAG+TGG 0.510095 3.4:-22807261 MS.gene86729:CDS
AATGCCGTCCCCTTGAGGGT+TGG 0.510127 3.4:+22807036 None:intergenic
AGCCTTAGCTCAAGAGAACT+TGG 0.510474 3.4:-22807316 MS.gene86729:CDS
CACCAAGTTCTCTTGAGCTA+AGG 0.512371 3.4:+22807314 None:intergenic
CAGCCACCATTAAAGGAGTT+CGG 0.512886 3.4:+22808883 None:intergenic
GAGAAGAGCCTGATGTAGGT+TGG 0.517531 3.4:-22807144 MS.gene86729:CDS
GTGCCAGCTCTTCATTTACC+AGG 0.519169 3.4:-22807853 MS.gene86729:CDS
GAATGGACCCAACCTACATC+AGG 0.520252 3.4:+22807136 None:intergenic
AAGACAGCAGATTTGTGTGT+TGG 0.520972 3.4:+22807556 None:intergenic
GCCTTGTCCGATACTGAGCT+GGG 0.524492 3.4:+22808139 None:intergenic
ATTGTTTCCATGGTCAAAAG+AGG 0.526082 3.4:+22807168 None:intergenic
AAGAGAGACCATACTGAGCC+TGG 0.528223 3.4:+22807446 None:intergenic
GGGGATAGAGGCTGTGCAAA+GGG 0.528233 3.4:+22807913 None:intergenic
CTGAGATTTCTTCATCCCCT+CGG 0.532952 3.4:-22807612 MS.gene86729:CDS
ATATGTTGCAAGACTTTGAT+GGG 0.533657 3.4:-22807756 MS.gene86729:CDS
TTGGGTCCATTCCCTTGTCA+AGG 0.537395 3.4:-22807125 MS.gene86729:CDS
CTCGATGAAATAAGGGACAT+TGG 0.538353 3.4:+22808543 None:intergenic
ATTATTGCCATTTGCAGATG+GGG 0.539077 3.4:+22807894 None:intergenic
GGGGACCCAACAGCTGAGAC+AGG 0.540557 3.4:+22807283 None:intergenic
ACCTTCAGCCGCAGATGAAG+AGG 0.541690 3.4:+22809103 None:intergenic
AAAAGAATCCCAACCCTCAA+GGG 0.542914 3.4:-22807045 MS.gene86729:CDS
TGATCAATGCCGTCCCCTTG+AGG 0.543662 3.4:+22807031 None:intergenic
TTCAGAAGATGGAATCGGGC+CGG 0.544086 3.4:+22807066 None:intergenic
CATCATGTAGCGGCATATCA+CGG 0.544466 3.4:+22807788 None:intergenic
GGCTACACCCAGCTCAGTAT+CGG 0.547166 3.4:-22808146 MS.gene86729:CDS
TAGCAGTAGAAGCAGCTGAT+CGG 0.547990 3.4:+22808010 None:intergenic
ATCATGTAGCGGCATATCAC+GGG 0.549825 3.4:+22807789 None:intergenic
GATATGTGTGAATATGCTCA+TGG 0.550252 3.4:-22808183 MS.gene86729:CDS
CTCTCTGACAGTGCTTCTGA+AGG 0.553902 3.4:-22808588 MS.gene86729:CDS
CGGACAAGGCTCTGCAAAGA+CGG 0.555061 3.4:-22808126 MS.gene86729:CDS
TAGGATATCAATACTCCCAT+AGG 0.558003 3.4:+22808857 None:intergenic
TTCAAGCCTTCGAGCTTATG+GGG 0.559064 3.4:+22808630 None:intergenic
GACATGACATTTGTGTTGAT+GGG 0.559180 3.4:+22807499 None:intergenic
GAAGCAGCTGATCGGGGTGA+AGG 0.560891 3.4:+22808018 None:intergenic
ATGGTCTCTCTTCTCCTGGA+AGG 0.562685 3.4:-22807435 MS.gene86729:CDS
TGGGATCCTTGACAAGGGAA+TGG 0.563496 3.4:+22807119 None:intergenic
GTTTCCATGGTCAAAAGAGG+TGG 0.566182 3.4:+22807171 None:intergenic
TACTGAGCCTGGTGGAATAG+GGG 0.568600 3.4:+22807457 None:intergenic
CGACCGACTGAAATAGCACC+AGG 0.568814 3.4:+22807688 None:intergenic
GACAGCAGATTTGTGTGTTG+GGG 0.570940 3.4:+22807558 None:intergenic
ATCATCTTCCTCTTCATCTG+CGG 0.571871 3.4:-22809111 MS.gene86729:CDS
AGGCTCTGCAAAGACGGTAT+GGG 0.573441 3.4:-22808120 MS.gene86729:CDS
CGTCCTGGTGCTATTTCAGT+CGG 0.576475 3.4:-22807691 MS.gene86729:CDS
GAGAAATTAACATCAGCCTC+AGG 0.578710 3.4:+22808816 None:intergenic
TGCTTCTACTGCTAATGTCA+TGG 0.583456 3.4:-22808001 MS.gene86729:CDS
AATCCCAACCCTCAAGGGGA+CGG 0.584903 3.4:-22807040 MS.gene86729:CDS
TGGGTCTCCATCCTCTCCTG+TGG 0.587335 3.4:-22808484 MS.gene86729:CDS
CCGAGAACTCATTGGAGAGA+GGG 0.587752 3.4:+22807390 None:intergenic
GAAAAGAATCCCAACCCTCA+AGG 0.588830 3.4:-22807046 MS.gene86729:CDS
AGCATTAACCGAACCACTTG+AGG 0.589777 3.4:+22808755 None:intergenic
GATAAAGGAGCAGTTGAACC+AGG 0.590443 3.4:+22808519 None:intergenic
GTTGTTCCCCATAAGCTCGA+AGG 0.592106 3.4:-22808636 MS.gene86729:CDS
AGAGCTGGCACATTGGGCTG+TGG 0.592460 3.4:+22807865 None:intergenic
AATGGAGAAGAGCCTGATGT+AGG 0.593112 3.4:-22807148 MS.gene86729:CDS
TGTAGAATTAACAGCTGAGT+CGG 0.595082 3.4:+22808449 None:intergenic
TGAGAGTATGCACGAGAACA+AGG 0.597066 3.4:+22808327 None:intergenic
CGGCAGGGTCAGAATACCGA+GGG 0.597848 3.4:+22807596 None:intergenic
AGATGATCCCCACTTAGACC+AGG 0.599519 3.4:+22807252 None:intergenic
AGCTGTAGTGAAACTTTCTA+GGG 0.601845 3.4:+22808250 None:intergenic
CAGGTGTGGGATCCTTGACA+AGG 0.602748 3.4:+22807113 None:intergenic
GACATCAAAGAGATTGATGA+TGG 0.604648 3.4:+22807943 None:intergenic
ACGGTATGGGTTGTAACCGA+AGG 0.606397 3.4:-22808107 MS.gene86729:CDS
TGGGGATAGAGGCTGTGCAA+AGG 0.609012 3.4:+22807912 None:intergenic
TATTGGAAAAGCTGATTGGT+CGG 0.609121 3.4:-22807227 MS.gene86729:CDS
GTTAATTGAAGGATGTGCGG+TGG 0.611177 3.4:-22809164 None:intergenic
CGGTATGGGTTGTAACCGAA+GGG 0.616814 3.4:-22808106 MS.gene86729:CDS
AAAATACAGAAGCCGCGGCA+GGG 0.617946 3.4:+22807581 None:intergenic
ACTTAGACCAGGAGTTAACA+GGG 0.618370 3.4:+22807263 None:intergenic
CGACTGAAATAGCACCAGGA+CGG 0.619234 3.4:+22807692 None:intergenic
TAAATGAAGAGCTGGCACAT+TGG 0.622782 3.4:+22807858 None:intergenic
TCAGTTAATTGAAGGATGTG+CGG 0.623237 3.4:-22809167 None:intergenic
TGAAGGAACAGCAGATCCAG+TGG 0.625826 3.4:+22808035 None:intergenic
GCTGATGTTAATTTCTCCTG+TGG 0.627824 3.4:-22808810 MS.gene86729:CDS
TACTGCTAATGTCATGGACA+TGG 0.629491 3.4:-22807995 MS.gene86729:CDS
GTTAACTCCTGGTCTAAGTG+GGG 0.631889 3.4:-22807259 MS.gene86729:CDS
GCAGTAGAAGCAGCTGATCG+GGG 0.631967 3.4:+22808012 None:intergenic
ACCGAGAACTCATTGGAGAG+AGG 0.634355 3.4:+22807389 None:intergenic
GCACCGAACTCCTTTAATGG+TGG 0.636101 3.4:-22808886 MS.gene86729:CDS
TCGGTAAATCCAAATGACTT+CGG 0.640900 3.4:-22807208 MS.gene86729:CDS
ATATGTGTGAATATGCTCAT+GGG 0.645475 3.4:-22808182 MS.gene86729:CDS
AGAGCATCCCCTATTCCACC+AGG 0.647565 3.4:-22807464 MS.gene86729:CDS
ATCCTGGAGAGAATGCTCGA+AGG 0.652913 3.4:-22808278 MS.gene86729:CDS
ACTGAAATAGCACCAGGACG+GGG 0.653843 3.4:+22807694 None:intergenic
ACCCAGCTCAGTATCGGACA+AGG 0.655881 3.4:-22808140 MS.gene86729:CDS
AAAGAATCCCAACCCTCAAG+GGG 0.657105 3.4:-22807044 MS.gene86729:CDS
GCTTATACTCTCATATCCTG+AGG 0.665811 3.4:-22808832 MS.gene86729:CDS
TGGGGAAAATACAGAAGCCG+CGG 0.666181 3.4:+22807576 None:intergenic
AGATTGATGATGGTGAACCA+GGG 0.666625 3.4:+22807953 None:intergenic
TCGGTATTCTGACCCTGCCG+CGG 0.669451 3.4:-22807593 MS.gene86729:CDS
TTGTTGAAGTCATCATGTAG+CGG 0.680309 3.4:+22807778 None:intergenic
GAACCTTGAATGAATACATG+CGG 0.683334 3.4:+22808352 None:intergenic
AGGTGTGGGATCCTTGACAA+GGG 0.686726 3.4:+22807114 None:intergenic
ATACATGCGGAATTCATCTG+TGG 0.687672 3.4:+22808365 None:intergenic
GCGGCAGGGTCAGAATACCG+AGG 0.701862 3.4:+22807595 None:intergenic
AGAGACCATACTGAGCCTGG+TGG 0.702718 3.4:+22807449 None:intergenic
TGGTGCTGATATCAATTCGG+TGG 0.710852 3.4:-22808703 MS.gene86729:CDS
TATGTGTGAATATGCTCATG+GGG 0.717472 3.4:-22808181 MS.gene86729:CDS
GAACAACAATAACATCGACA+GGG 0.720452 3.4:+22808664 None:intergenic
GATCCAGTGGACACATACAG+CGG 0.722026 3.4:+22808048 None:intergenic
CTTAGACCAGGAGTTAACAG+GGG 0.765437 3.4:+22807264 None:intergenic
GGCAGGGTCAGAATACCGAG+GGG 0.768209 3.4:+22807597 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AATAGATCATCAAGATTTGA+TGG + chr3.4:22808491-22808510 None:intergenic 25.0%
! GAAATCTTCAAAATCATTGT+TGG + chr3.4:22807133-22807152 None:intergenic 25.0%
!!! ATAAAATTGCTTTTATCAGC+TGG - chr3.4:22807405-22807424 MS.gene86729:CDS 25.0%
!!! CAATGATTTTGAAGATTTCA+AGG - chr3.4:22807134-22807153 MS.gene86729:CDS 25.0%
AATATGTTGCAAGACTTTGA+TGG - chr3.4:22808371-22808390 MS.gene86729:CDS 30.0%
ATATGTGTGAATATGCTCAT+GGG - chr3.4:22807946-22807965 MS.gene86729:CDS 30.0%
ATATGTTGCAAGACTTTGAT+GGG - chr3.4:22808372-22808391 MS.gene86729:CDS 30.0%
CTTTATCATCTGCTGAAAAT+GGG - chr3.4:22807625-22807644 MS.gene86729:CDS 30.0%
GCTTCACTTATTAATGAAGT+TGG - chr3.4:22807174-22807193 MS.gene86729:CDS 30.0%
TAAGCTGGAAAAAGAATCAT+CGG + chr3.4:22807097-22807116 None:intergenic 30.0%
! GATATGAGAGTATAAGCTTT+AGG + chr3.4:22807293-22807312 None:intergenic 30.0%
! TTATTAATGAAGTTGGCTTC+TGG - chr3.4:22807181-22807200 MS.gene86729:CDS 30.0%
! TTCTTTTCAGAAGATGGAAT+CGG + chr3.4:22809070-22809089 None:intergenic 30.0%
! TTTTTATGTCAGGAAGAGAT+GGG + chr3.4:22807731-22807750 None:intergenic 30.0%
!!! TACATGCTGTTTTTTATGTC+AGG + chr3.4:22807741-22807760 None:intergenic 30.0%
!!! TTTTTTATGTCAGGAAGAGA+TGG + chr3.4:22807732-22807751 None:intergenic 30.0%
AGACATGACATTTGTGTTGA+TGG + chr3.4:22808633-22808652 None:intergenic 35.0%
AGCTGTAGTGAAACTTTCTA+GGG + chr3.4:22807881-22807900 None:intergenic 35.0%
ATTCCGCATGTATTCATTCA+AGG - chr3.4:22807773-22807792 MS.gene86729:CDS 35.0%
ATTGTTTCCATGGTCAAAAG+AGG + chr3.4:22808963-22808982 None:intergenic 35.0%
CCTTTATCATCTGCTGAAAA+TGG - chr3.4:22807624-22807643 MS.gene86729:CDS 35.0%
CTAGTTTGATTAATGCTTCC+TGG + chr3.4:22808296-22808315 None:intergenic 35.0%
GAACAACAATAACATCGACA+GGG + chr3.4:22807467-22807486 None:intergenic 35.0%
GAACCTTGAATGAATACATG+CGG + chr3.4:22807779-22807798 None:intergenic 35.0%
GACATCAAAGAGATTGATGA+TGG + chr3.4:22808188-22808207 None:intergenic 35.0%
GATATGTGTGAATATGCTCA+TGG - chr3.4:22807945-22807964 MS.gene86729:CDS 35.0%
TAGGATATCAATACTCCCAT+AGG + chr3.4:22807274-22807293 None:intergenic 35.0%
TATGTGTGAATATGCTCATG+GGG - chr3.4:22807947-22807966 MS.gene86729:CDS 35.0%
TATTGGAAAAGCTGATTGGT+CGG - chr3.4:22808901-22808920 MS.gene86729:CDS 35.0%
TCGGTAAATCCAAATGACTT+CGG - chr3.4:22808920-22808939 MS.gene86729:CDS 35.0%
TGTAGAATTAACAGCTGAGT+CGG + chr3.4:22807682-22807701 None:intergenic 35.0%
TTGTTGAAGTCATCATGTAG+CGG + chr3.4:22808353-22808372 None:intergenic 35.0%
! ATTATTGCCATTTGCAGATG+GGG + chr3.4:22808237-22808256 None:intergenic 35.0%
! ATTTGTGTTGATGGGTGATA+AGG + chr3.4:22808624-22808643 None:intergenic 35.0%
! CATTATTGCCATTTGCAGAT+GGG + chr3.4:22808238-22808257 None:intergenic 35.0%
! CCATTTTCAGCAGATGATAA+AGG + chr3.4:22807627-22807646 None:intergenic 35.0%
! GACATGACATTTGTGTTGAT+GGG + chr3.4:22808632-22808651 None:intergenic 35.0%
! TCTTTTCAGAAGATGGAATC+GGG + chr3.4:22809069-22809088 None:intergenic 35.0%
! TCTTTTGACCATGGAAACAA+TGG - chr3.4:22808962-22808981 MS.gene86729:CDS 35.0%
! TTGGGATTCTTTTCAGAAGA+TGG + chr3.4:22809076-22809095 None:intergenic 35.0%
! TTTGGTTAGATCCCTTTTGA+CGG + chr3.4:22807212-22807231 None:intergenic 35.0%
!! ATGCCCTGATTTTAGAAAAG+GGG - chr3.4:22807908-22807927 MS.gene86729:CDS 35.0%
!! CATGCCCTGATTTTAGAAAA+GGG - chr3.4:22807907-22807926 MS.gene86729:CDS 35.0%
!!! AAGCCCCTTTTCTAAAATCA+GGG + chr3.4:22807914-22807933 None:intergenic 35.0%
!!! ATGGAGTTAGTGTTTTAGAG+CGG + chr3.4:22808472-22808491 None:intergenic 35.0%
!!! GAAAGTGATTTTGATTCGTC+AGG + chr3.4:22806991-22807010 None:intergenic 35.0%
AAAAGAATCCCAACCCTCAA+GGG - chr3.4:22809083-22809102 MS.gene86729:CDS 40.0%
AAATGAAGAGCTGGCACATT+GGG + chr3.4:22808272-22808291 None:intergenic 40.0%
AACCAGGACTCGATGAAATA+AGG + chr3.4:22807596-22807615 None:intergenic 40.0%
AAGACAGCAGATTTGTGTGT+TGG + chr3.4:22808575-22808594 None:intergenic 40.0%
ACCAGGACTCGATGAAATAA+GGG + chr3.4:22807595-22807614 None:intergenic 40.0%
ACTTAGACCAGGAGTTAACA+GGG + chr3.4:22808868-22808887 None:intergenic 40.0%
AGACAGCAGATTTGTGTGTT+GGG + chr3.4:22808574-22808593 None:intergenic 40.0%
AGCTTATGGGGAACAACAAT+AGG + chr3.4:22807489-22807508 None:intergenic 40.0%
ATACATGCGGAATTCATCTG+TGG + chr3.4:22807766-22807785 None:intergenic 40.0%
ATCATCTTCCTCTTCATCTG+CGG - chr3.4:22807017-22807036 MS.gene86729:CDS 40.0%
CAGCTGTAGTGAAACTTTCT+AGG + chr3.4:22807882-22807901 None:intergenic 40.0%
CTCCTATTGGAAAAGCTGAT+TGG - chr3.4:22808897-22808916 MS.gene86729:CDS 40.0%
CTCGATGAAATAAGGGACAT+TGG + chr3.4:22807588-22807607 None:intergenic 40.0%
CTGCAAATGGCAATAATGCT+TGG - chr3.4:22808240-22808259 MS.gene86729:CDS 40.0%
GAGAAATTAACATCAGCCTC+AGG + chr3.4:22807315-22807334 None:intergenic 40.0%
GCTGATGTTAATTTCTCCTG+TGG - chr3.4:22807318-22807337 MS.gene86729:CDS 40.0%
GCTTATACTCTCATATCCTG+AGG - chr3.4:22807296-22807315 MS.gene86729:CDS 40.0%
GGAACAACAATAACATCGAC+AGG + chr3.4:22807468-22807487 None:intergenic 40.0%
TAAATGAAGAGCTGGCACAT+TGG + chr3.4:22808273-22808292 None:intergenic 40.0%
TACTGCTAATGTCATGGACA+TGG - chr3.4:22808133-22808152 MS.gene86729:CDS 40.0%
TGCTTCTACTGCTAATGTCA+TGG - chr3.4:22808127-22808146 MS.gene86729:CDS 40.0%
TGTGTTTGACCGAAGTCATT+TGG + chr3.4:22808932-22808951 None:intergenic 40.0%
TGTTAACTCCTGGTCTAAGT+GGG - chr3.4:22808868-22808887 MS.gene86729:CDS 40.0%
TTCTCTTTCTTCTCAGGTGT+GGG + chr3.4:22809031-22809050 None:intergenic 40.0%
TTCTGGTATGTCCGTCAAAA+GGG - chr3.4:22807198-22807217 MS.gene86729:CDS 40.0%
TTTCAAGCCTTCGAGCTTAT+GGG + chr3.4:22807502-22807521 None:intergenic 40.0%
! ACAGCTGAGTCGGTAAATTT+TGG + chr3.4:22807672-22807691 None:intergenic 40.0%
! AGTGCTTTTATCAGTTCCAC+AGG + chr3.4:22807337-22807356 None:intergenic 40.0%
! ATCAATTCGGTGGATGCTAA+TGG - chr3.4:22807435-22807454 MS.gene86729:CDS 40.0%
! GACCAATCAGCTTTTCCAAT+AGG + chr3.4:22808902-22808921 None:intergenic 40.0%
! GCATTATTGCCATTTGCAGA+TGG + chr3.4:22808239-22808258 None:intergenic 40.0%
! TCAATTCGGTGGATGCTAAT+GGG - chr3.4:22807436-22807455 MS.gene86729:CDS 40.0%
!! AGATTGATGATGGTGAACCA+GGG + chr3.4:22808178-22808197 None:intergenic 40.0%
!! AGCTGGTGCTGATATCAATT+CGG - chr3.4:22807422-22807441 MS.gene86729:CDS 40.0%
!! AGCTTTGAAGCTGTTGAAAC+TGG + chr3.4:22808601-22808620 None:intergenic 40.0%
!! CCATGCCCTGATTTTAGAAA+AGG - chr3.4:22807906-22807925 MS.gene86729:CDS 40.0%
!! TTCGGTGCTCAAGAACAATT+TGG + chr3.4:22807230-22807249 None:intergenic 40.0%
!! TTTTCAAGCCTTCGAGCTTA+TGG + chr3.4:22807503-22807522 None:intergenic 40.0%
!!! CAAGCCCCTTTTCTAAAATC+AGG + chr3.4:22807915-22807934 None:intergenic 40.0%
!!! CCTTTTCTAAAATCAGGGCA+TGG + chr3.4:22807909-22807928 None:intergenic 40.0%
!!! GGCATTGATCACTCTGTTTT+GGG - chr3.4:22809109-22809128 MS.gene86729:CDS 40.0%
AAAGAATCCCAACCCTCAAG+GGG - chr3.4:22809084-22809103 MS.gene86729:CDS 45.0%
AAAGAGAAGCTTGCAGGTTC+CGG - chr3.4:22809043-22809062 MS.gene86729:CDS 45.0%
AACAATCCTGTCTCAGCTGT+TGG - chr3.4:22808839-22808858 MS.gene86729:CDS 45.0%
AAGGATGTCGCCTAGAAGTA+TGG - chr3.4:22808712-22808731 MS.gene86729:CDS 45.0%
AAGTGGGGATCATCTCCTAT+TGG - chr3.4:22808884-22808903 MS.gene86729:CDS 45.0%
AATGGAGAAGAGCCTGATGT+AGG - chr3.4:22808980-22808999 MS.gene86729:CDS 45.0%
ACAATCCTGTCTCAGCTGTT+GGG - chr3.4:22808840-22808859 MS.gene86729:CDS 45.0%
AGAAGAGCCTGATGTAGGTT+GGG - chr3.4:22808985-22809004 MS.gene86729:CDS 45.0%
AGCATTAACCGAACCACTTG+AGG + chr3.4:22807376-22807395 None:intergenic 45.0%
AGCCTTAGCTCAAGAGAACT+TGG - chr3.4:22808812-22808831 MS.gene86729:CDS 45.0%
ATACTGAGCCTGGTGGAATA+GGG + chr3.4:22808675-22808694 None:intergenic 45.0%
ATCATGTAGCGGCATATCAC+GGG + chr3.4:22808342-22808361 None:intergenic 45.0%
CACCAAGTTCTCTTGAGCTA+AGG + chr3.4:22808817-22808836 None:intergenic 45.0%
CACTTAGACCAGGAGTTAAC+AGG + chr3.4:22808869-22808888 None:intergenic 45.0%
CAGCCACCATTAAAGGAGTT+CGG + chr3.4:22807248-22807267 None:intergenic 45.0%
CATCATGTAGCGGCATATCA+CGG + chr3.4:22808343-22808362 None:intergenic 45.0%
CTCGTGCATACTCTCATGAT+TGG - chr3.4:22807808-22807827 MS.gene86729:CDS 45.0%
CTGAGATTTCTTCATCCCCT+CGG - chr3.4:22808516-22808535 MS.gene86729:CDS 45.0%
CTGTTAACTCCTGGTCTAAG+TGG - chr3.4:22808867-22808886 MS.gene86729:CDS 45.0%
CTTAGACCAGGAGTTAACAG+GGG + chr3.4:22808867-22808886 None:intergenic 45.0%
CTTCCTGGTAAATGAAGAGC+TGG + chr3.4:22808281-22808300 None:intergenic 45.0%
CTTCTCTTTCTTCTCAGGTG+TGG + chr3.4:22809032-22809051 None:intergenic 45.0%
CTTCTGGTATGTCCGTCAAA+AGG - chr3.4:22807197-22807216 MS.gene86729:CDS 45.0%
GAAAAGAATCCCAACCCTCA+AGG - chr3.4:22809082-22809101 MS.gene86729:CDS 45.0%
GACAGCAGATTTGTGTGTTG+GGG + chr3.4:22808573-22808592 None:intergenic 45.0%
GAGAAGAAAGAGAAGCTTGC+AGG - chr3.4:22809037-22809056 MS.gene86729:CDS 45.0%
GATAAAGGAGCAGTTGAACC+AGG + chr3.4:22807612-22807631 None:intergenic 45.0%
GCAAGCTTCTCTTTCTTCTC+AGG + chr3.4:22809037-22809056 None:intergenic 45.0%
GCTCTTCTCCATTGTTTCCA+TGG + chr3.4:22808973-22808992 None:intergenic 45.0%
GTTAACTCCTGGTCTAAGTG+GGG - chr3.4:22808869-22808888 MS.gene86729:CDS 45.0%
GTTTCCATGGTCAAAAGAGG+TGG + chr3.4:22808960-22808979 None:intergenic 45.0%
TAATGGTGGCTGCTTCCTAT+GGG - chr3.4:22807256-22807275 MS.gene86729:CDS 45.0%
TAGCAGTAGAAGCAGCTGAT+CGG + chr3.4:22808121-22808140 None:intergenic 45.0%
TAGGAAGCAGCCACCATTAA+AGG + chr3.4:22807255-22807274 None:intergenic 45.0%
TTAATGGTGGCTGCTTCCTA+TGG - chr3.4:22807255-22807274 MS.gene86729:CDS 45.0%
TTCAAGCCTTCGAGCTTATG+GGG + chr3.4:22807501-22807520 None:intergenic 45.0%
TTCTCTCCAGGATGCACAAA+AGG + chr3.4:22807843-22807862 None:intergenic 45.0%
! AGATCCACCTCTTTTGACCA+TGG - chr3.4:22808953-22808972 MS.gene86729:CDS 45.0%
! ATGATGGTGAACCAGGGAAA+AGG + chr3.4:22808172-22808191 None:intergenic 45.0%
! TCCCTTATTTCATCGAGTCC+TGG - chr3.4:22807591-22807610 MS.gene86729:CDS 45.0%
! TGAGAGTATGCACGAGAACA+AGG + chr3.4:22807804-22807823 None:intergenic 45.0%
!! GAGATTGATGATGGTGAACC+AGG + chr3.4:22808179-22808198 None:intergenic 45.0%
!! TGAGCACCGAACTCCTTTAA+TGG - chr3.4:22807239-22807258 MS.gene86729:CDS 45.0%
!! TGGTGCTGATATCAATTCGG+TGG - chr3.4:22807425-22807444 MS.gene86729:CDS 45.0%
!!! AAATTTTGGAGCCACAGGAG+AGG + chr3.4:22807658-22807677 None:intergenic 45.0%
!!! ATCACTCTGTTTTGGGAGCT+TGG - chr3.4:22809116-22809135 MS.gene86729:CDS 45.0%
!!! CGGCATTGATCACTCTGTTT+TGG - chr3.4:22809108-22809127 MS.gene86729:CDS 45.0%
!!! TCGGTAAATTTTGGAGCCAC+AGG + chr3.4:22807663-22807682 None:intergenic 45.0%
AAAAGGGGCTTGCAGACGTT+CGG - chr3.4:22807923-22807942 MS.gene86729:CDS 50.0%
AAAATACAGAAGCCGCGGCA+GGG + chr3.4:22808550-22808569 None:intergenic 50.0%
AAGAGAGACCATACTGAGCC+TGG + chr3.4:22808685-22808704 None:intergenic 50.0%
AAGGCTCTGCAAAGACGGTA+TGG - chr3.4:22808007-22808026 MS.gene86729:CDS 50.0%
ACCGAGAACTCATTGGAGAG+AGG + chr3.4:22808742-22808761 None:intergenic 50.0%
ACTGAAATAGCACCAGGACG+GGG + chr3.4:22808437-22808456 None:intergenic 50.0%
AGATGATCCCCACTTAGACC+AGG + chr3.4:22808879-22808898 None:intergenic 50.0%
AGCAGTAGAAGCAGCTGATC+GGG + chr3.4:22808120-22808139 None:intergenic 50.0%
AGGCTCTGCAAAGACGGTAT+GGG - chr3.4:22808008-22808027 MS.gene86729:CDS 50.0%
AGGGGATGCTCTACGTTTGT+AGG + chr3.4:22808656-22808675 None:intergenic 50.0%
AGGTGTGGGATCCTTGACAA+GGG + chr3.4:22809017-22809036 None:intergenic 50.0%
ATCCTGGAGAGAATGCTCGA+AGG - chr3.4:22807850-22807869 MS.gene86729:CDS 50.0%
ATGGTCTCTCTTCTCCTGGA+AGG - chr3.4:22808693-22808712 MS.gene86729:CDS 50.0%
CAGTATGGTCTCTCTTCTCC+TGG - chr3.4:22808689-22808708 MS.gene86729:CDS 50.0%
CATACTGAGCCTGGTGGAAT+AGG + chr3.4:22808676-22808695 None:intergenic 50.0%
CCATTTGCAGATGGGGATAG+AGG + chr3.4:22808230-22808249 None:intergenic 50.0%
CCCTCTCTCCAATGAGTTCT+CGG - chr3.4:22808738-22808757 MS.gene86729:CDS 50.0%
CCGAGAACTCATTGGAGAGA+GGG + chr3.4:22808741-22808760 None:intergenic 50.0%
CCTCTATCCCCATCTGCAAA+TGG - chr3.4:22808227-22808246 MS.gene86729:CDS 50.0%
CGACTGAAATAGCACCAGGA+CGG + chr3.4:22808439-22808458 None:intergenic 50.0%
CTATTCCACCAGGCTCAGTA+TGG - chr3.4:22808674-22808693 MS.gene86729:CDS 50.0%
CTCTCTGACAGTGCTTCTGA+AGG - chr3.4:22807540-22807559 MS.gene86729:CDS 50.0%
GAATGGACCCAACCTACATC+AGG + chr3.4:22808995-22809014 None:intergenic 50.0%
GACTGAAATAGCACCAGGAC+GGG + chr3.4:22808438-22808457 None:intergenic 50.0%
GAGAAGAGCCTGATGTAGGT+TGG - chr3.4:22808984-22809003 MS.gene86729:CDS 50.0%
GAGAGGGCTTCCATACTTCT+AGG + chr3.4:22808725-22808744 None:intergenic 50.0%
GATCCAGTGGACACATACAG+CGG + chr3.4:22808083-22808102 None:intergenic 50.0%
GCACCGAACTCCTTTAATGG+TGG - chr3.4:22807242-22807261 MS.gene86729:CDS 50.0%
GCTGACAACCGAGAACTCAT+TGG + chr3.4:22808749-22808768 None:intergenic 50.0%
GTGCCAGCTCTTCATTTACC+AGG - chr3.4:22808275-22808294 MS.gene86729:CDS 50.0%
GTTGTTCCCCATAAGCTCGA+AGG - chr3.4:22807492-22807511 MS.gene86729:CDS 50.0%
TACTGAGCCTGGTGGAATAG+GGG + chr3.4:22808674-22808693 None:intergenic 50.0%
TCAGGAAGAGATGGGTCAAC+TGG + chr3.4:22807723-22807742 None:intergenic 50.0%
TGAAGGAACAGCAGATCCAG+TGG + chr3.4:22808096-22808115 None:intergenic 50.0%
TGGGATCCTTGACAAGGGAA+TGG + chr3.4:22809012-22809031 None:intergenic 50.0%
TGGGGAAAATACAGAAGCCG+CGG + chr3.4:22808555-22808574 None:intergenic 50.0%
TTCAGAAGATGGAATCGGGC+CGG + chr3.4:22809065-22809084 None:intergenic 50.0%
TTGGGTCCATTCCCTTGTCA+AGG - chr3.4:22809003-22809022 MS.gene86729:CDS 50.0%
! ACGGTATGGGTTGTAACCGA+AGG - chr3.4:22808021-22808040 MS.gene86729:CDS 50.0%
! CGGTATGGGTTGTAACCGAA+GGG - chr3.4:22808022-22808041 MS.gene86729:CDS 50.0%
! CTCATGGGGTATTTGAGTGC+TGG - chr3.4:22807961-22807980 MS.gene86729:CDS 50.0%
! GAGCGAAAAAGCACACCCTT+CGG + chr3.4:22808040-22808059 None:intergenic 50.0%
! GAGTGTCCTTTTGTGCATCC+TGG - chr3.4:22807834-22807853 MS.gene86729:CDS 50.0%
! GCTATTTCAGTCGGTCGATC+TGG - chr3.4:22808446-22808465 MS.gene86729:CDS 50.0%
! GGAAGCATGTTCGAGTAAGC+TGG + chr3.4:22807112-22807131 None:intergenic 50.0%
!! CGTCCTGGTGCTATTTCAGT+CGG - chr3.4:22808437-22808456 MS.gene86729:CDS 50.0%
AATCCCAACCCTCAAGGGGA+CGG - chr3.4:22809088-22809107 MS.gene86729:CDS 55.0%
ACCTTCAGCCGCAGATGAAG+AGG + chr3.4:22807028-22807047 None:intergenic 55.0%
AGAGACCATACTGAGCCTGG+TGG + chr3.4:22808682-22808701 None:intergenic 55.0%
CAGGTGTGGGATCCTTGACA+AGG + chr3.4:22809018-22809037 None:intergenic 55.0%
CGACCGACTGAAATAGCACC+AGG + chr3.4:22808443-22808462 None:intergenic 55.0%
CGGACAAGGCTCTGCAAAGA+CGG - chr3.4:22808002-22808021 MS.gene86729:CDS 55.0%
CTCCTTCGAGCATTCTCTCC+AGG + chr3.4:22807855-22807874 None:intergenic 55.0%
CTGTGCTGCCTCAAGTGGTT+CGG - chr3.4:22807365-22807384 MS.gene86729:CDS 55.0%
CTTCACTGTGCTGCCTCAAG+TGG - chr3.4:22807360-22807379 MS.gene86729:CDS 55.0%
CTTCTAGGCGACATCCTTCC+AGG + chr3.4:22808710-22808729 None:intergenic 55.0%
GAAAATACAGAAGCCGCGGC+AGG + chr3.4:22808551-22808570 None:intergenic 55.0%
GATCAATGCCGTCCCCTTGA+GGG + chr3.4:22809099-22809118 None:intergenic 55.0%
GCAGTAGAAGCAGCTGATCG+GGG + chr3.4:22808119-22808138 None:intergenic 55.0%
GCCTTGTCCGATACTGAGCT+GGG + chr3.4:22807992-22808011 None:intergenic 55.0%
GGCTACACCCAGCTCAGTAT+CGG - chr3.4:22807982-22808001 MS.gene86729:CDS 55.0%
GGGGATAGAGGCTGTGCAAA+GGG + chr3.4:22808218-22808237 None:intergenic 55.0%
TCCTCTTCATCTGCGGCTGA+AGG - chr3.4:22807024-22807043 MS.gene86729:CDS 55.0%
TGATCAATGCCGTCCCCTTG+AGG + chr3.4:22809100-22809119 None:intergenic 55.0%
TGGGGATAGAGGCTGTGCAA+AGG + chr3.4:22808219-22808238 None:intergenic 55.0%
TTGGGTCCCCTGTTAACTCC+TGG - chr3.4:22808858-22808877 MS.gene86729:CDS 55.0%
! ACCCAGCTCAGTATCGGACA+AGG - chr3.4:22807988-22808007 MS.gene86729:CDS 55.0%
! AGAGCATCCCCTATTCCACC+AGG - chr3.4:22808664-22808683 MS.gene86729:CDS 55.0%
! AGCCTTGTCCGATACTGAGC+TGG + chr3.4:22807993-22808012 None:intergenic 55.0%
! GCTGCTATGAGCCTTTTCCC+TGG - chr3.4:22808158-22808177 MS.gene86729:CDS 55.0%
! TTGGCTTGAGCAACTGCAGC+TGG - chr3.4:22809135-22809154 MS.gene86729:CDS 55.0%
! TTTGGAGCCACAGGAGAGGA+TGG + chr3.4:22807654-22807673 None:intergenic 55.0%
AATGCCGTCCCCTTGAGGGT+TGG + chr3.4:22809095-22809114 None:intergenic 60.0%
AGAGCTGGCACATTGGGCTG+TGG + chr3.4:22808266-22808285 None:intergenic 60.0%
ATGCCGTCCCCTTGAGGGTT+GGG + chr3.4:22809094-22809113 None:intergenic 60.0%
CGGCAGGGTCAGAATACCGA+GGG + chr3.4:22808535-22808554 None:intergenic 60.0%
CGTCCGCTGTATGTGTCCAC+TGG - chr3.4:22808077-22808096 MS.gene86729:CDS 60.0%
GAAGCAGCTGATCGGGGTGA+AGG + chr3.4:22808113-22808132 None:intergenic 60.0%
GGCAGGGTCAGAATACCGAG+GGG + chr3.4:22808534-22808553 None:intergenic 60.0%
TACAGCGGACGCAGCTCTTC+AGG + chr3.4:22808068-22808087 None:intergenic 60.0%
TCGGTATTCTGACCCTGCCG+CGG - chr3.4:22808535-22808554 MS.gene86729:CDS 60.0%
TGGGTCTCCATCCTCTCCTG+TGG - chr3.4:22807644-22807663 MS.gene86729:CDS 60.0%
TGTTTCTCACAGCCCCGTCC+TGG - chr3.4:22808422-22808441 MS.gene86729:CDS 60.0%
GCGGCAGGGTCAGAATACCG+AGG + chr3.4:22808536-22808555 None:intergenic 65.0%
GGGGACCCAACAGCTGAGAC+AGG + chr3.4:22808848-22808867 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 22806976 22809174 22806976 ID=MS.gene86729
chr3.4 mRNA 22806976 22809174 22806976 ID=MS.gene86729.t1;Parent=MS.gene86729
chr3.4 exon 22806976 22809174 22806976 ID=MS.gene86729.t1.exon1;Parent=MS.gene86729.t1
chr3.4 CDS 22806976 22809174 22806976 ID=cds.MS.gene86729.t1;Parent=MS.gene86729.t1
Gene Sequence

>MS.gene86729

ATGTGCGGTGGACCTGACGAATCAAAATCACTTTCAACTTCATCATCTTCCTCTTCATCTGCGGCTGAAGGTTCAACTTCAAATACTGTCAAAGACATGAAAAATCTAACTGTTCGTACCGATGATTCTTTTTCCAGCTTACTCGAACATGCTTCCAACAATGATTTTGAAGATTTCAAGGTAGCTCTAGATAGTGATGCTTCACTTATTAATGAAGTTGGCTTCTGGTATGTCCGTCAAAAGGGATCTAACCAAATTGTTCTTGAGCACCGAACTCCTTTAATGGTGGCTGCTTCCTATGGGAGTATTGATATCCTAAAGCTTATACTCTCATATCCTGAGGCTGATGTTAATTTCTCCTGTGGAACTGATAAAAGCACTGCTCTTCACTGTGCTGCCTCAAGTGGTTCGGTTAATGCTGTTGACGCTATAAAATTGCTTTTATCAGCTGGTGCTGATATCAATTCGGTGGATGCTAATGGGAAACGCCCTGTCGATGTTATTGTTGTTCCTATTGTTGTTCCCCATAAGCTCGAAGGCTTGAAAACAATTCTTGAAGAACTTCTCTCTGACAGTGCTTCTGAAGGATCTGTTGATGATTGCTCTCTTCCAATGTCCCTTATTTCATCGAGTCCTGGTTCAACTGCTCCTTTATCATCTGCTGAAAATGGGTCTCCATCCTCTCCTGTGGCTCCAAAATTTACCGACTCAGCTGTTAATTCTACATCAGAGAAGAAAGAGTATCCAGTTGACCCATCTCTTCCTGACATAAAAAACAGCATGTATGCCACAGATGAATTCCGCATGTATTCATTCAAGGTTCGTCCTTGTTCTCGTGCATACTCTCATGATTGGACAGAGTGTCCTTTTGTGCATCCTGGAGAGAATGCTCGAAGGAGAGACCCTAGAAAGTTTCACTACAGCTGTGTGCCATGCCCTGATTTTAGAAAAGGGGCTTGCAGACGTTCGGATATGTGTGAATATGCTCATGGGGTATTTGAGTGCTGGCTACACCCAGCTCAGTATCGGACAAGGCTCTGCAAAGACGGTATGGGTTGTAACCGAAGGGTGTGCTTTTTCGCTCACTCACCTGAAGAGCTGCGTCCGCTGTATGTGTCCACTGGATCTGCTGTTCCTTCACCCCGATCAGCTGCTTCTACTGCTAATGTCATGGACATGGCTGCTGCTATGAGCCTTTTCCCTGGTTCACCATCATCAATCTCTTTGATGTCTCAATCACCCTTTGCACAGCCTCTATCCCCATCTGCAAATGGCAATAATGCTTGGCCACAGCCCAATGTGCCAGCTCTTCATTTACCAGGAAGCATTAATCAAACTAGTCGTTTGAGATCTTCTCTCAGTGCCCGTGATATGCCGCTACATGATGACTTCAACAATATGTTGCAAGACTTTGATGGGCAGCAGCAGATTCTGAATGACTTGAGCTGTTTCTCACAGCCCCGTCCTGGTGCTATTTCAGTCGGTCGATCTGGCCGCTCTAAAACACTAACTCCATCAAATCTTGATGATCTATTTTGTGCTGAGATTTCTTCATCCCCTCGGTATTCTGACCCTGCCGCGGCTTCTGTATTTTCCCCAACACACAAATCTGCTGTCTTCAACCAGTTTCAACAGCTTCAAAGCTCCTTATCACCCATCAACACAAATGTCATGTCTCCTACAAACGTAGAGCATCCCCTATTCCACCAGGCTCAGTATGGTCTCTCTTCTCCTGGAAGGATGTCGCCTAGAAGTATGGAAGCCCTCTCTCCAATGAGTTCTCGGTTGTCAGCTTTTGCTCAGCGTGAGAAACAGCAGCAGCAGCAACAGCTGCGTAGCCTTAGCTCAAGAGAACTTGGTGCTAACAATCCTGTCTCAGCTGTTGGGTCCCCTGTTAACTCCTGGTCTAAGTGGGGATCATCTCCTATTGGAAAAGCTGATTGGTCGGTAAATCCAAATGACTTCGGTCAAACACAGAGATCCACCTCTTTTGACCATGGAAACAATGGAGAAGAGCCTGATGTAGGTTGGGTCCATTCCCTTGTCAAGGATCCCACACCTGAGAAGAAAGAGAAGCTTGCAGGTTCCGGCCCGATTCCATCTTCTGAAAAGAATCCCAACCCTCAAGGGGACGGCATTGATCACTCTGTTTTGGGAGCTTGGCTTGAGCAACTGCAGCTGGATCAACTTGTAGTCTAG
Protein sequence

>MS.gene86729.t1

MCGGPDESKSLSTSSSSSSSAAEGSTSNTVKDMKNLTVRTDDSFSSLLEHASNNDFEDFKVALDSDASLINEVGFWYVRQKGSNQIVLEHRTPLMVAASYGSIDILKLILSYPEADVNFSCGTDKSTALHCAASSGSVNAVDAIKLLLSAGADINSVDANGKRPVDVIVVPIVVPHKLEGLKTILEELLSDSASEGSVDDCSLPMSLISSSPGSTAPLSSAENGSPSSPVAPKFTDSAVNSTSEKKEYPVDPSLPDIKNSMYATDEFRMYSFKVRPCSRAYSHDWTECPFVHPGENARRRDPRKFHYSCVPCPDFRKGACRRSDMCEYAHGVFECWLHPAQYRTRLCKDGMGCNRRVCFFAHSPEELRPLYVSTGSAVPSPRSAASTANVMDMAAAMSLFPGSPSSISLMSQSPFAQPLSPSANGNNAWPQPNVPALHLPGSINQTSRLRSSLSARDMPLHDDFNNMLQDFDGQQQILNDLSCFSQPRPGAISVGRSGRSKTLTPSNLDDLFCAEISSSPRYSDPAAASVFSPTHKSAVFNQFQQLQSSLSPINTNVMSPTNVEHPLFHQAQYGLSSPGRMSPRSMEALSPMSSRLSAFAQREKQQQQQQLRSLSSRELGANNPVSAVGSPVNSWSKWGSSPIGKADWSVNPNDFGQTQRSTSFDHGNNGEEPDVGWVHSLVKDPTPEKKEKLAGSGPIPSSEKNPNPQGDGIDHSVLGAWLEQLQLDQLVV