AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene86916

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene86916.t1 MTR_1g106470 69.805 308 58 3 1 295 1 286 1.47e-141 400
MS.gene86916.t1 MTR_1g106470 64.093 259 58 3 1 246 1 237 2.41e-102 299
MS.gene86916.t1 MTR_5g005110 45.994 337 124 12 1 299 1 317 4.79e-72 225
MS.gene86916.t1 MTR_1g106480 69.412 170 8 3 156 295 1 156 1.77e-68 210
MS.gene86916.t1 MTR_1g106460 40.533 338 151 10 1 297 1 329 8.64e-61 196
MS.gene86916.t1 MTR_3g103030 51.724 174 77 3 121 289 142 313 2.32e-48 164
MS.gene86916.t1 MTR_1g106490 67.925 106 28 2 23 124 24 127 2.71e-40 137
MS.gene86916.t1 MTR_1g018090 81.818 77 14 0 204 280 273 349 3.58e-38 138
MS.gene86916.t1 MTR_5g005110 39.643 280 116 10 1 244 1 263 5.13e-38 135
MS.gene86916.t1 MTR_1g106460 32.746 284 142 9 1 243 1 276 4.90e-27 107
MS.gene86916.t1 MTR_5g079500 52.809 89 41 1 185 273 199 286 2.54e-24 100
MS.gene86916.t1 MTR_8g093500 55.072 69 31 0 201 269 110 178 8.69e-18 81.6
MS.gene86916.t1 MTR_3g101810 42.056 107 56 2 195 299 163 265 3.39e-17 80.1
MS.gene86916.t1 MTR_4g131160 56.716 67 29 0 202 268 193 259 3.74e-17 81.3
MS.gene86916.t1 MTR_4g131160 56.716 67 29 0 202 268 196 262 4.02e-17 81.3
MS.gene86916.t1 MTR_1g080890 53.425 73 34 0 196 268 184 256 1.45e-16 79.3
MS.gene86916.t1 MTR_7g069590 56.364 55 24 0 212 266 33 87 3.29e-15 72.0
MS.gene86916.t1 MTR_1g018090 72.727 44 12 0 204 247 273 316 7.63e-14 71.2
MS.gene86916.t1 MTR_1g084980 50.877 57 28 0 212 268 469 525 9.31e-14 72.0
MS.gene86916.t1 MTR_1g019240 38.824 85 50 1 198 282 80 162 1.11e-13 70.1
MS.gene86916.t1 MTR_3g449770 41.772 79 36 2 201 274 300 373 7.44e-13 68.9
MS.gene86916.t1 MTR_7g053410 47.368 76 39 1 194 268 306 381 2.28e-12 67.4
MS.gene86916.t1 MTR_7g053410 47.368 76 39 1 194 268 306 381 2.35e-12 67.4
MS.gene86916.t1 MTR_5g017040 39.706 68 41 0 214 281 114 181 5.22e-12 65.9
MS.gene86916.t1 MTR_8g012290 38.614 101 52 2 194 293 280 371 2.70e-11 64.3
MS.gene86916.t1 MTR_8g012290 38.614 101 52 2 194 293 280 371 2.77e-11 64.3
MS.gene86916.t1 MTR_7g099540 45.614 57 31 0 212 268 259 315 2.97e-11 63.9
MS.gene86916.t1 MTR_1g070870 47.423 97 48 2 175 268 90 186 4.45e-11 62.8
MS.gene86916.t1 MTR_1g069155 38.889 72 44 0 197 268 309 380 7.46e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene86916.t1 AT2G14760 39.692 325 156 9 10 296 6 328 8.96e-50 168
MS.gene86916.t1 AT2G14760 38.166 338 156 10 10 296 6 341 4.72e-46 159
MS.gene86916.t1 AT4G33880 36.494 348 146 12 10 287 6 348 3.14e-44 154
MS.gene86916.t1 AT5G43175 71.134 97 28 0 191 287 118 214 3.98e-44 150
MS.gene86916.t1 AT1G27740 73.563 87 23 0 201 287 163 249 1.59e-41 144
MS.gene86916.t1 AT5G37800 50.000 98 44 2 176 273 177 269 7.85e-23 96.3
MS.gene86916.t1 AT1G66470 58.333 72 29 1 202 273 193 263 6.25e-22 93.6
MS.gene86916.t1 AT2G24260 51.852 81 39 0 199 279 29 109 2.19e-19 85.9
MS.gene86916.t1 AT2G24260 58.571 70 29 0 199 268 132 201 7.64e-19 85.9
MS.gene86916.t1 AT4G30980 57.143 70 30 0 199 268 124 193 1.46e-18 84.7
MS.gene86916.t1 AT4G30980 57.143 70 30 0 199 268 172 241 1.74e-18 85.1
MS.gene86916.t1 AT5G58010 44.898 98 52 1 173 268 65 162 2.70e-17 80.9
MS.gene86916.t1 AT3G21330 49.383 81 36 2 187 267 256 331 1.94e-16 79.3
MS.gene86916.t1 AT5G01305 51.613 62 30 0 203 264 35 96 7.28e-16 74.3
MS.gene86916.t1 AT4G00120 53.704 54 25 0 214 267 121 174 2.96e-15 73.2
MS.gene86916.t1 AT1G09530 43.939 66 37 0 212 277 344 409 3.18e-13 70.1
MS.gene86916.t1 AT1G09530 43.939 66 37 0 212 277 344 409 3.18e-13 70.1
MS.gene86916.t1 AT1G09530 43.939 66 37 0 212 277 344 409 3.18e-13 70.1
MS.gene86916.t1 AT1G09530 43.939 66 37 0 212 277 344 409 3.18e-13 70.1
MS.gene86916.t1 AT1G09530 43.939 66 37 0 212 277 344 409 3.18e-13 70.1
MS.gene86916.t1 AT1G09530 43.939 66 37 0 212 277 344 409 3.18e-13 70.1
MS.gene86916.t1 AT4G36930 37.838 74 46 0 212 285 198 271 1.39e-12 67.8
MS.gene86916.t1 AT5G50915 34.395 157 91 5 141 289 65 217 1.54e-12 67.0
MS.gene86916.t1 AT5G50915 34.395 157 91 5 141 289 65 217 1.54e-12 67.0
MS.gene86916.t1 AT5G50915 34.395 157 91 5 141 289 65 217 1.69e-12 66.6
MS.gene86916.t1 AT5G50915 34.395 157 91 5 141 289 65 217 1.69e-12 66.6
MS.gene86916.t1 AT3G23690 44.737 76 41 1 194 268 180 255 1.76e-11 64.7
MS.gene86916.t1 AT2G20180 47.368 57 30 0 212 268 214 270 2.03e-11 64.3
MS.gene86916.t1 AT2G20180 47.368 57 30 0 212 268 214 270 2.03e-11 64.3
MS.gene86916.t1 AT2G20180 47.368 57 30 0 212 268 214 270 2.03e-11 64.3
MS.gene86916.t1 AT2G20180 47.368 57 30 0 212 268 285 341 2.11e-11 64.7
MS.gene86916.t1 AT2G20180 47.368 57 30 0 212 268 285 341 2.11e-11 64.7
MS.gene86916.t1 AT2G20180 47.368 57 30 0 212 268 285 341 2.11e-11 64.7
MS.gene86916.t1 AT2G20180 47.368 57 30 0 212 268 285 341 2.11e-11 64.7
MS.gene86916.t1 AT5G48560 46.053 76 40 1 194 268 290 365 2.31e-11 64.7
MS.gene86916.t1 AT5G48560 46.053 76 40 1 194 268 290 365 2.54e-11 64.3
MS.gene86916.t1 AT4G00050 46.154 52 28 0 221 272 121 172 5.80e-11 62.4

Find 63 sgRNAs with CRISPR-Local

Find 107 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAATTTAGTCCACAATATT+TGG 0.147520 1.2:-79233163 MS.gene86916:CDS
CAAATATTGTGGACTAAATT+TGG 0.154244 1.2:+79233164 None:intergenic
TGGCTCATAGAACCAGATTT+TGG 0.215361 1.2:+79232760 None:intergenic
TTCTCTTCTCCAACTAAATT+AGG 0.240816 1.2:-79231864 MS.gene86916:CDS
GCTTACAATGGAATAAATAT+TGG 0.247349 1.2:-79231900 MS.gene86916:CDS
TGGAACCCATTCATGTGATT+TGG 0.268183 1.2:-79233232 MS.gene86916:CDS
CCCATTCTTCATAAATCAAT+TGG 0.283233 1.2:+79233364 None:intergenic
CTACGCAACTTAGGTGCTTC+AGG 0.318498 1.2:+79232625 None:intergenic
CAGAAAATATAAGGAATGTT+AGG 0.319597 1.2:-79232789 MS.gene86916:CDS
CCCAATTGATTTATGAAGAA+TGG 0.324327 1.2:-79233365 MS.gene86916:CDS
TGTAGGTTTCAGAAAATATA+AGG 0.339371 1.2:-79232798 MS.gene86916:intron
TTTATTCCATTGTAAGCAAT+TGG 0.346999 1.2:+79231906 None:intergenic
GGAACCCATTCATGTGATTT+GGG 0.350013 1.2:-79233231 MS.gene86916:CDS
ATAAGATGGCATGGCAAAAC+TGG 0.354371 1.2:+79233269 None:intergenic
GACTTCATGGCTCAGTTCCT+TGG 0.360528 1.2:-79233306 MS.gene86916:CDS
AAATCTACCTTAGTTCCATT+TGG 0.360868 1.2:+79232212 None:intergenic
ATTCATCTTCCTAATTTAGT+TGG 0.363312 1.2:+79231855 None:intergenic
GTTAGGAAATGTCCAAAATC+TGG 0.392760 1.2:-79232772 MS.gene86916:CDS
GAAGAATGGGATTCTCTTAG+TGG 0.396492 1.2:-79233351 MS.gene86916:CDS
TGAAAACAATTTGCGTTTGC+GGG 0.398395 1.2:-79233073 MS.gene86916:CDS
TTGTTCCCAAATCACATGAA+TGG 0.399061 1.2:+79233226 None:intergenic
GCTCAGTTCCTTGGTTGTAA+TGG 0.403811 1.2:-79233297 MS.gene86916:CDS
CCAATTGATTTATGAAGAAT+GGG 0.414473 1.2:-79233364 MS.gene86916:CDS
TTGCAAACTCTTGTTCCAAA+TGG 0.419165 1.2:-79232227 MS.gene86916:CDS
AGCCGAGGAAGCTGACTTCA+TGG 0.435912 1.2:-79233319 MS.gene86916:CDS
TGTTCCCAAATCACATGAAT+GGG 0.437898 1.2:+79233227 None:intergenic
TGTTGGAGCCATGCCACGTT+AGG 0.453646 1.2:+79232725 None:intergenic
ACTAAATTTGGCATCTTCCA+TGG 0.457758 1.2:+79233176 None:intergenic
TCTTGTTCCAAATGGAACTA+AGG 0.473754 1.2:-79232219 MS.gene86916:intron
TTGAAAACAATTTGCGTTTG+CGG 0.474421 1.2:-79233074 MS.gene86916:CDS
CCATTCTTCATAAATCAATT+GGG 0.483050 1.2:+79233365 None:intergenic
GAATGGGTTCCATTACTTGA+AGG 0.488758 1.2:+79233243 None:intergenic
TACGAATGCATTAATCATGA+AGG 0.493341 1.2:-79233114 MS.gene86916:CDS
ATGCATTAATCATGAAGGGC+CGG 0.500325 1.2:-79233109 MS.gene86916:CDS
AACCTGTGGAAAACAGTTGT+TGG 0.508340 1.2:+79232708 None:intergenic
TGCATTAATCATGAAGGGCC+GGG 0.510310 1.2:-79233108 MS.gene86916:CDS
ATGCTCTGTGCATCAGTGGC+AGG 0.521454 1.2:+79232580 None:intergenic
GAAGAGAAAGAATAAATGAA+AGG 0.522466 1.2:-79232259 MS.gene86916:CDS
TTTGCGTTTGCGGGATGAGA+GGG 0.532509 1.2:-79233064 MS.gene86916:CDS
TTACTTGAAGGAAAATAAGA+TGG 0.542752 1.2:+79233255 None:intergenic
AGCCATGAAGTCAGCTTCCT+CGG 0.543459 1.2:+79233317 None:intergenic
AAGTTATGATCCAAATATTG+TGG 0.544900 1.2:+79233153 None:intergenic
TGAAGGAAAATAAGATGGCA+TGG 0.574425 1.2:+79233260 None:intergenic
GAAGAGGGCCTAACGTGGCA+TGG 0.579675 1.2:-79232733 MS.gene86916:CDS
AGAAAATCGAAGGCAGGAAG+TGG 0.581938 1.2:-79232604 MS.gene86916:CDS
ACGTTAGGCCCTCTTCATTG+TGG 0.582294 1.2:+79232740 None:intergenic
TTGCGTAGAAAATCGAAGGC+AGG 0.583966 1.2:-79232610 MS.gene86916:CDS
AATGATGATGACTTCAGAAG+AGG 0.597972 1.2:-79233034 MS.gene86916:CDS
TCTATGAGCCACAATGAAGA+GGG 0.604923 1.2:-79232748 MS.gene86916:CDS
TATGCTCCAATTGCTTACAA+TGG 0.606083 1.2:-79231912 MS.gene86916:CDS
GGATATTAGTACCATGCTTG+AGG 0.607564 1.2:-79232095 MS.gene86916:CDS
TTCTATGAGCCACAATGAAG+AGG 0.608991 1.2:-79232749 MS.gene86916:CDS
TAGTTGGAGAAGAGAAGCTG+AGG 0.609048 1.2:+79231871 None:intergenic
ATTTGCGTTTGCGGGATGAG+AGG 0.609289 1.2:-79233065 MS.gene86916:CDS
ACGAATGCATTAATCATGAA+GGG 0.612142 1.2:-79233113 MS.gene86916:CDS
TTGGACAGCTTCCTCAAGCA+TGG 0.619471 1.2:+79232084 None:intergenic
TAAGTTGCGTAGAAAATCGA+AGG 0.620676 1.2:-79232614 MS.gene86916:CDS
TGGTGTTTCCATTACAACCA+AGG 0.631490 1.2:+79233289 None:intergenic
AAGATTTAGGAGCTCACTAG+AGG 0.634903 1.2:-79232980 MS.gene86916:intron
AAAGGATTAGAGTTAACCTG+TGG 0.678724 1.2:+79232694 None:intergenic
ACAATGAAGAGGGCCTAACG+TGG 0.686185 1.2:-79232738 MS.gene86916:CDS
ATAAATGCTCTGTGCATCAG+TGG 0.693217 1.2:+79232576 None:intergenic
TAGTGGACAGTACACAGCCG+AGG 0.694851 1.2:-79233334 MS.gene86916:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTCATTGTTTATTTATTTAT+TGG - chr1.2:79233259-79233278 MS.gene86916:CDS 10.0%
!! ATTGAACTATTACTCAATAA+TGG - chr1.2:79233063-79233082 MS.gene86916:CDS 20.0%
!! GCATATAAGAAATTGAAAAT+TGG + chr1.2:79233223-79233242 None:intergenic 20.0%
!! TTGAACTATTACTCAATAAT+GGG - chr1.2:79233064-79233083 MS.gene86916:CDS 20.0%
!!! TAATCATGATTTTACTATGT+AGG - chr1.2:79232417-79232436 MS.gene86916:intron 20.0%
!!! TAGAGGTATAATAATAGTTA+AGG - chr1.2:79232269-79232288 MS.gene86916:intron 20.0%
!!! TTTTTTTTGTTGTGAATTGT+AGG - chr1.2:79233113-79233132 MS.gene86916:CDS 20.0%
! AAGTTATGATCCAAATATTG+TGG + chr1.2:79232082-79232101 None:intergenic 25.0%
! ACATTTCTTTAGTGCATATT+TGG - chr1.2:79232703-79232722 MS.gene86916:CDS 25.0%
! AGTTAAATGTCTCTCATTAT+AGG - chr1.2:79232923-79232942 MS.gene86916:intron 25.0%
! CAAATTTAGTCCACAATATT+TGG - chr1.2:79232069-79232088 MS.gene86916:CDS 25.0%
! CAGAAAATATAAGGAATGTT+AGG - chr1.2:79232443-79232462 MS.gene86916:intron 25.0%
! CCATTCTTCATAAATCAATT+GGG + chr1.2:79231870-79231889 None:intergenic 25.0%
! GAAGAGAAAGAATAAATGAA+AGG - chr1.2:79232973-79232992 MS.gene86916:intron 25.0%
! GCTTACAATGGAATAAATAT+TGG - chr1.2:79233332-79233351 MS.gene86916:CDS 25.0%
! TGTAGGTTTCAGAAAATATA+AGG - chr1.2:79232434-79232453 MS.gene86916:intron 25.0%
! TTACTTGAAGGAAAATAAGA+TGG + chr1.2:79231980-79231999 None:intergenic 25.0%
! TTTATTCCATTGTAAGCAAT+TGG + chr1.2:79233329-79233348 None:intergenic 25.0%
!! ACAATATGTTACCATGATTT+TGG - chr1.2:79232749-79232768 MS.gene86916:CDS 25.0%
!! CAAATATTGTGGACTAAATT+TGG + chr1.2:79232071-79232090 None:intergenic 25.0%
!! CCAATTGATTTATGAAGAAT+GGG - chr1.2:79231868-79231887 MS.gene86916:CDS 25.0%
!! CGTAATTTTTACCAAAATCA+TGG + chr1.2:79232763-79232782 None:intergenic 25.0%
!! TCTTTTAGGCTTAAAATTGA+AGG + chr1.2:79232586-79232605 None:intergenic 25.0%
!!! TCTTATTTTCCTTCAAGTAA+TGG - chr1.2:79231980-79231999 MS.gene86916:intron 25.0%
AAATCTACCTTAGTTCCATT+TGG + chr1.2:79233023-79233042 None:intergenic 30.0%
ACAGTAGTCAGTAGTTTAAT+TGG + chr1.2:79232880-79232899 None:intergenic 30.0%
ACGAATGCATTAATCATGAA+GGG - chr1.2:79232119-79232138 MS.gene86916:intron 30.0%
ACTAAAGAAATGTCCTTCAA+AGG + chr1.2:79232696-79232715 None:intergenic 30.0%
AGAGGATAAAAGCAAAAACT+TGG - chr1.2:79232216-79232235 MS.gene86916:intron 30.0%
ATCTTCCATGGAAAAAAACA+TGG + chr1.2:79232047-79232066 None:intergenic 30.0%
CCCATTCTTCATAAATCAAT+TGG + chr1.2:79231871-79231890 None:intergenic 30.0%
CTAAAGAAATGTCCTTCAAA+GGG + chr1.2:79232695-79232714 None:intergenic 30.0%
GAACTAAGGTAGATTTCTTA+AGG - chr1.2:79233027-79233046 MS.gene86916:CDS 30.0%
GAGGATAAAAGCAAAAACTT+GGG - chr1.2:79232217-79232236 MS.gene86916:intron 30.0%
TACGAATGCATTAATCATGA+AGG - chr1.2:79232118-79232137 MS.gene86916:intron 30.0%
TTCTCTTCTCCAACTAAATT+AGG - chr1.2:79233368-79233387 MS.gene86916:CDS 30.0%
TTGAAAACAATTTGCGTTTG+CGG - chr1.2:79232158-79232177 MS.gene86916:intron 30.0%
! CCCAATTGATTTATGAAGAA+TGG - chr1.2:79231867-79231886 MS.gene86916:CDS 30.0%
! GCATTCGTAGTTTATCTTTT+TGG + chr1.2:79232107-79232126 None:intergenic 30.0%
! GTTTTTGCAGCTTCAAATTA+AGG - chr1.2:79233179-79233198 MS.gene86916:CDS 30.0%
! TAAACTACTGACTACTGTAT+TGG - chr1.2:79232882-79232901 MS.gene86916:intron 30.0%
! TAAGTGCGTGTTTAGAATTA+AGG + chr1.2:79232829-79232848 None:intergenic 30.0%
! TTTTCAACAAGAACTAAACC+CGG + chr1.2:79232145-79232164 None:intergenic 30.0%
!! TACTCAATAATGGGTTAAGA+TGG - chr1.2:79233073-79233092 MS.gene86916:CDS 30.0%
!! TTTTTGTTGTGAATTGTAGG+TGG - chr1.2:79233116-79233135 MS.gene86916:CDS 30.0%
AAAGGATTAGAGTTAACCTG+TGG + chr1.2:79232541-79232560 None:intergenic 35.0%
ACTAAATTTGGCATCTTCCA+TGG + chr1.2:79232059-79232078 None:intergenic 35.0%
CATGGAAAAAAACATGGACA+AGG + chr1.2:79232041-79232060 None:intergenic 35.0%
GAAACCCTGCAAAAAGATTT+AGG - chr1.2:79232239-79232258 MS.gene86916:CDS 35.0%
GCTGCAAAAACTTCACATAT+TGG + chr1.2:79233170-79233189 None:intergenic 35.0%
GTGAATTTGATGAATCACGA+TGG + chr1.2:79232807-79232826 None:intergenic 35.0%
GTTAGGAAATGTCCAAAATC+TGG - chr1.2:79232460-79232479 MS.gene86916:intron 35.0%
TAAGTTGCGTAGAAAATCGA+AGG - chr1.2:79232618-79232637 MS.gene86916:CDS 35.0%
TATGCTCCAATTGCTTACAA+TGG - chr1.2:79233320-79233339 MS.gene86916:CDS 35.0%
TCTTGTTCCAAATGGAACTA+AGG - chr1.2:79233013-79233032 MS.gene86916:CDS 35.0%
TGAAAACAATTTGCGTTTGC+GGG - chr1.2:79232159-79232178 MS.gene86916:intron 35.0%
TGAAGGAAAATAAGATGGCA+TGG + chr1.2:79231975-79231994 None:intergenic 35.0%
TGTTCCCAAATCACATGAAT+GGG + chr1.2:79232008-79232027 None:intergenic 35.0%
TTGCAAACTCTTGTTCCAAA+TGG - chr1.2:79233005-79233024 MS.gene86916:CDS 35.0%
TTGTTCCCAAATCACATGAA+TGG + chr1.2:79232009-79232028 None:intergenic 35.0%
! AATGATGATGACTTCAGAAG+AGG - chr1.2:79232198-79232217 MS.gene86916:intron 35.0%
! AGCTCCTAAATCTTTTTGCA+GGG + chr1.2:79232246-79232265 None:intergenic 35.0%
! GGTTAACTCTAATCCTTTTC+TGG - chr1.2:79232543-79232562 MS.gene86916:intron 35.0%
! TTCGATTTTCTACGCAACTT+AGG + chr1.2:79232619-79232638 None:intergenic 35.0%
!!! AATCCTTTTCTGGAGCAAAA+TGG - chr1.2:79232553-79232572 MS.gene86916:intron 35.0%
!!! CTTGTCCATGTTTTTTTCCA+TGG - chr1.2:79232039-79232058 MS.gene86916:intron 35.0%
!!! TTTTGAGCTCTGAAGATATG+TGG - chr1.2:79233294-79233313 MS.gene86916:CDS 35.0%
AACCTGTGGAAAACAGTTGT+TGG + chr1.2:79232527-79232546 None:intergenic 40.0%
AAGATTTAGGAGCTCACTAG+AGG - chr1.2:79232252-79232271 MS.gene86916:CDS 40.0%
ATAAATGCTCTGTGCATCAG+TGG + chr1.2:79232659-79232678 None:intergenic 40.0%
ATAAGATGGCATGGCAAAAC+TGG + chr1.2:79231966-79231985 None:intergenic 40.0%
ATGCATTAATCATGAAGGGC+CGG - chr1.2:79232123-79232142 MS.gene86916:intron 40.0%
CAAGAGTAAGTTCCCTTTGA+AGG - chr1.2:79232680-79232699 MS.gene86916:CDS 40.0%
GAAGAATGGGATTCTCTTAG+TGG - chr1.2:79231881-79231900 MS.gene86916:CDS 40.0%
GGAACCCATTCATGTGATTT+GGG - chr1.2:79232001-79232020 MS.gene86916:intron 40.0%
GGATATTAGTACCATGCTTG+AGG - chr1.2:79233137-79233156 MS.gene86916:CDS 40.0%
TCTATGAGCCACAATGAAGA+GGG - chr1.2:79232484-79232503 MS.gene86916:intron 40.0%
TGGAACCCATTCATGTGATT+TGG - chr1.2:79232000-79232019 MS.gene86916:intron 40.0%
TGGTGTTTCCATTACAACCA+AGG + chr1.2:79231946-79231965 None:intergenic 40.0%
TTCTATGAGCCACAATGAAG+AGG - chr1.2:79232483-79232502 MS.gene86916:intron 40.0%
! GAATGGGTTCCATTACTTGA+AGG + chr1.2:79231992-79232011 None:intergenic 40.0%
! GAGCTCCTAAATCTTTTTGC+AGG + chr1.2:79232247-79232266 None:intergenic 40.0%
! TGGCTCATAGAACCAGATTT+TGG + chr1.2:79232475-79232494 None:intergenic 40.0%
! TTTCCTCATGTTTTGTGCAG+AGG - chr1.2:79232949-79232968 MS.gene86916:intron 40.0%
!! TAGGTGCTTCAGGTTCTTTT+AGG + chr1.2:79232600-79232619 None:intergenic 40.0%
AGAAAATCGAAGGCAGGAAG+TGG - chr1.2:79232628-79232647 MS.gene86916:CDS 45.0%
GCTCAGTTCCTTGGTTGTAA+TGG - chr1.2:79231935-79231954 MS.gene86916:CDS 45.0%
TAGTTGGAGAAGAGAAGCTG+AGG + chr1.2:79233364-79233383 None:intergenic 45.0%
TCTCCTCTGCACAAAACATG+AGG + chr1.2:79232955-79232974 None:intergenic 45.0%
TGCATTAATCATGAAGGGCC+GGG - chr1.2:79232124-79232143 MS.gene86916:intron 45.0%
TTGCGTAGAAAATCGAAGGC+AGG - chr1.2:79232622-79232641 MS.gene86916:CDS 45.0%
! CCTCCATTTTGCTCCAGAAA+AGG + chr1.2:79232559-79232578 None:intergenic 45.0%
! CTCCAACAACTGTTTTCCAC+AGG - chr1.2:79232522-79232541 MS.gene86916:intron 45.0%
!!! CCTTTTCTGGAGCAAAATGG+AGG - chr1.2:79232556-79232575 MS.gene86916:intron 45.0%
ACAATGAAGAGGGCCTAACG+TGG - chr1.2:79232494-79232513 MS.gene86916:intron 50.0%
ACGTTAGGCCCTCTTCATTG+TGG + chr1.2:79232495-79232514 None:intergenic 50.0%
AGCCATGAAGTCAGCTTCCT+CGG + chr1.2:79231918-79231937 None:intergenic 50.0%
ATTTGCGTTTGCGGGATGAG+AGG - chr1.2:79232167-79232186 MS.gene86916:intron 50.0%
CTACGCAACTTAGGTGCTTC+AGG + chr1.2:79232610-79232629 None:intergenic 50.0%
GACTTCATGGCTCAGTTCCT+TGG - chr1.2:79231926-79231945 MS.gene86916:CDS 50.0%
TTGGACAGCTTCCTCAAGCA+TGG + chr1.2:79233151-79233170 None:intergenic 50.0%
TTTGCGTTTGCGGGATGAGA+GGG - chr1.2:79232168-79232187 MS.gene86916:intron 50.0%
ATGCTCTGTGCATCAGTGGC+AGG + chr1.2:79232655-79232674 None:intergenic 55.0%
TGTTGGAGCCATGCCACGTT+AGG + chr1.2:79232510-79232529 None:intergenic 55.0%
! AGCCGAGGAAGCTGACTTCA+TGG - chr1.2:79231913-79231932 MS.gene86916:CDS 55.0%
! TAGTGGACAGTACACAGCCG+AGG - chr1.2:79231898-79231917 MS.gene86916:CDS 55.0%
GAAGAGGGCCTAACGTGGCA+TGG - chr1.2:79232499-79232518 MS.gene86916:intron 60.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 79231857 79233397 79231857 ID=MS.gene86916
chr1.2 mRNA 79231857 79233397 79231857 ID=MS.gene86916.t1;Parent=MS.gene86916
chr1.2 exon 79232981 79233397 79232981 ID=MS.gene86916.t1.exon1;Parent=MS.gene86916.t1
chr1.2 CDS 79232981 79233397 79232981 ID=cds.MS.gene86916.t1;Parent=MS.gene86916.t1
chr1.2 exon 79232570 79232815 79232570 ID=MS.gene86916.t1.exon2;Parent=MS.gene86916.t1
chr1.2 CDS 79232570 79232815 79232570 ID=cds.MS.gene86916.t1;Parent=MS.gene86916.t1
chr1.2 exon 79232220 79232285 79232220 ID=MS.gene86916.t1.exon3;Parent=MS.gene86916.t1
chr1.2 CDS 79232220 79232285 79232220 ID=cds.MS.gene86916.t1;Parent=MS.gene86916.t1
chr1.2 exon 79232054 79232119 79232054 ID=MS.gene86916.t1.exon4;Parent=MS.gene86916.t1
chr1.2 CDS 79232054 79232119 79232054 ID=cds.MS.gene86916.t1;Parent=MS.gene86916.t1
chr1.2 exon 79231857 79231961 79231857 ID=MS.gene86916.t1.exon5;Parent=MS.gene86916.t1
chr1.2 CDS 79231857 79231961 79231857 ID=cds.MS.gene86916.t1;Parent=MS.gene86916.t1
Gene Sequence

>MS.gene86916

ATGGAATCTGCCCAATTGATTTATGAAGAATGGGATTCTCTTAGTGGACAGTACACAGCCGAGGAAGCTGACTTCATGGCTCAGTTCCTTGGTTGTAATGGAAACACCAGTTTTGCCATGCCATCTTATTTTCCTTCAAGTAATGGAACCCATTCATGTGATTTGGGAACAAATATTGATTCCTTGTCCATGTTTTTTTCCATGGAAGATGCCAAATTTAGTCCACAATATTTGGATCATAACTTAACCAAAAAGATAAACTACGAATGCATTAATCATGAAGGGCCGGGTTTAGTTCTTGTTGAAAACAATTTGCGTTTGCGGGATGAGAGGGAGCATGAAATGATGATGACTTCAGAAGAGGATAAAAGCAAAAACTTGGGAAACCCTGCAAAAAGATTTAGGAGCTCACTAGAGGTATAATAATAGTTAAGGTATTTATTATTGACAAAAAATAGTTGATGTTAAATTAAACTTTGAAAACGACAAATAAATAGAAATAACTTTTTCTACAAAAAAGCAACAAGTTTAGAAGCTACTACGTTCATTGTTTGAGATTTTAATCATGATTTTACTATGTAGGTTTCAGAAAATATAAGGAATGTTAGGAAATGTCCAAAATCTGGTTCTATGAGCCACAATGAAGAGGGCCTAACGTGGCATGGCTCCAACAACTGTTTTCCACAGGTTAACTCTAATCCTTTTCTGGAGCAAAATGGAGGAAAACCTTCAATTTTAAGCCTAAAAGAACCTGAAGCACCTAAGTTGCGTAGAAAATCGAAGGCAGGAAGTGGTCCTGCCACTGATGCACAGAGCATTTATGCAAGAGTAAGTTCCCTTTGAAGGACATTTCTTTAGTGCATATTTGGATTCAAGTGAGTTATGCTGAATCACAATATGTTACCATGATTTTGGTAAAAATTACGATTCAGATTTCAATCATTGTGCCATCGTGATTCATCAAATTCACCTTAATTCTAAACACGCACTTAATAAAATCATGATGAAGAATAGTACATGCCAATTAAACTACTGACTACTGTATTGGTAACATTTTCTTGCTTTTAGTTAAATGTCTCTCATTATAGGTTTTTTCCTCATGTTTTGTGCAGAGGAGAAGAGAAAGAATAAATGAAAGGTTGAGAATCTTGCAAACTCTTGTTCCAAATGGAACTAAGGTAGATTTCTTAAGGACAACAATATAATATTGAACTATTACTCAATAATGGGTTAAGATGGTCAAATTCTCAAACTTTTTTTTTTTGTTGTGAATTGTAGGTGGATATTAGTACCATGCTTGAGGAAGCTGTCCAATATGTGAAGTTTTTGCAGCTTCAAATTAAGGTAACAATTTGAGAAACTTCCAATTTTCAATTTCTTATATGCAAAACATAAATATTAATTCATTGTTTATTTATTTATTGGATATGAAACAGCTTTTGAGCTCTGAAGATATGTGGATGTATGCTCCAATTGCTTACAATGGAATAAATATTGGATTAGACCTCAGCTTCTCTTCTCCAACTAAATTAGGAAGATGA
Protein sequence

>MS.gene86916.t1

MESAQLIYEEWDSLSGQYTAEEADFMAQFLGCNGNTSFAMPSYFPSSNGTHSCDLGTNIDSLSMFFSMEDAKFSPQYLDHNLTKKINYECINHEGPGLVLVENNLRLRDEREHEMMMTSEEDKSKNLGNPAKRFRSSLEVSENIRNVRKCPKSGSMSHNEEGLTWHGSNNCFPQVNSNPFLEQNGGKPSILSLKEPEAPKLRRKSKAGSGPATDAQSIYARRRRERINERLRILQTLVPNGTKVDISTMLEEAVQYVKFLQLQIKLLSSEDMWMYAPIAYNGINIGLDLSFSSPTKLGR