AlfalfaGEDB | MS.gene86977

M. sativa cultivar XinJiangDaYe / MS.gene86977

KEGG: -
GO: GO:0003674 GO:0005488 GO:0005515 GO:0046983
PFAM: PF00010.29

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene86977.t1	XP_003597984.2	85.3	258	32	2	1	256	1	254	1.00E-106	396.4

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene86977.t1	Q9T072	37.1	202	118	4	55	249	105	304	2.7e-23	110.5

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene86977.t1	G7IMC5	85.3	258	32	2	1	256	1	254	7.5e-107	396.4

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene86977.t1	TF	bHLH

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene86977.t1	MTR_8g069740	79.478	268	37	3	1	251	1	267	2.04e-146	410
MS.gene86977.t1	MTR_2g104650	85.271	258	32	2	1	256	1	254	2.21e-145	408
MS.gene86977.t1	MTR_2g104590	59.690	258	52	6	1	256	2	209	4.09e-89	264
MS.gene86977.t1	MTR_2g104620	57.143	210	47	5	1	208	1	169	9.72e-56	176
MS.gene86977.t1	MTR_8g009020	47.514	181	86	4	84	256	129	308	1.39e-42	148
MS.gene86977.t1	MTR_2g104550	39.373	287	136	10	1	257	20	298	3.43e-42	146
MS.gene86977.t1	MTR_2g104490	37.729	273	144	8	4	254	23	291	9.29e-42	145
MS.gene86977.t1	MTR_2g104490	37.132	272	145	8	4	253	23	290	1.12e-40	142
MS.gene86977.t1	MTR_4g009540	40.865	208	112	4	60	256	109	316	4.68e-39	139
MS.gene86977.t1	MTR_5g014520	44.311	167	91	2	91	256	150	315	3.71e-38	136

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene86977.t1	AT4G37850	35.885	209	125	4	55	256	105	311	4.54e-33	123
MS.gene86977.t1	AT4G37850	35.885	209	125	4	55	256	105	311	4.54e-33	123
MS.gene86977.t1	AT2G22750	42.105	171	95	1	91	257	119	289	9.98e-31	116
MS.gene86977.t1	AT2G22750	44.172	163	87	1	91	249	119	281	1.35e-30	115
MS.gene86977.t1	AT2G22750	42.105	171	95	1	91	257	147	317	1.81e-30	116
MS.gene86977.t1	AT2G22750	42.105	171	95	1	91	257	143	313	1.93e-30	116
MS.gene86977.t1	AT2G22750	44.172	163	87	1	91	249	147	309	2.46e-30	115
MS.gene86977.t1	AT2G22760	33.136	169	110	3	91	256	112	280	1.89e-24	99.4
MS.gene86977.t1	AT2G22760	33.529	170	110	3	83	249	104	273	3.68e-24	98.6
MS.gene86977.t1	AT2G22770	34.731	167	94	3	98	249	132	298	2.18e-22	94.4

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Find 49 sgRNAs with CRISPR-Local

Find 75 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGTAAGATCATAGAACTTT+CGG	0.375512	2.3:+357646	MS.gene86977:CDS
AATGGCTGTGTAGATTGATA+AGG	0.531973	2.3:+357790	MS.gene86977:intron
AATGTCTCAGAAGCGCTTCG+AGG	0.506140	2.3:-357581	None:intergenic
ACATAATGTCGGAGAGGAAT+AGG	0.390434	2.3:+357609	MS.gene86977:CDS
ACGACTTGAAGAACGTTTGA+AGG	0.420593	2.3:+357847	MS.gene86977:CDS
ACTAGGCCAGTTGTGGCAAT+AGG	0.327076	2.3:-357407	None:intergenic
ACTTCTAGAGGTTGAAGCTA+GGG	0.520330	2.3:+357994	MS.gene86977:CDS
AGATGGATGAGTTGTTTACT+AGG	0.471822	2.3:-357424	None:intergenic
AGGTTGAAGCTAGGGTTCTT+GGG	0.420139	2.3:+358002	MS.gene86977:CDS
AGTACCAATGTCTTGCCATT+TGG	0.350019	2.3:+358118	MS.gene86977:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAGCCATTGCTAATCAATT+TGG	+	chr2.3:358422-358441	MS.gene86977:intron	30.0%
AGTGAAAAAACTAAGAGTAG+TGG	+	chr2.3:358325-358344	MS.gene86977:intron	30.0%
ATTAACAAAGACACTGCAAT+CGG	+	chr2.3:357938-357957	MS.gene86977:CDS	30.0%
GAAGTGTGCATTTAGTATTT+GGG	+	chr2.3:358381-358400	MS.gene86977:intron	30.0%
GACATTAGATCACATAATGT+CGG	+	chr2.3:357598-357617	MS.gene86977:CDS	30.0%
GTGTTAATCAAGATTCATTG+TGG	+	chr2.3:358031-358050	MS.gene86977:CDS	30.0%
TGAAGTGTGCATTTAGTATT+TGG	+	chr2.3:358380-358399	MS.gene86977:intron	30.0%
TGAATAATGTGACACATAGA+TGG	-	chr2.3:357444-357463	None:intergenic	30.0%
TGGAAGAAATTTAAGAAGCA+TGG	+	chr2.3:358213-358232	MS.gene86977:intron	30.0%
AATGGCTGTGTAGATTGATA+AGG	+	chr2.3:357790-357809	MS.gene86977:intron	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2.3	gene	357314	358482	357314	ID=MS.gene86977
chr2.3	mRNA	357314	358482	357314	ID=MS.gene86977.t1;Parent=MS.gene86977
chr2.3	exon	357314	357691	357314	ID=MS.gene86977.t1.exon1;Parent=MS.gene86977.t1
chr2.3	CDS	357314	357691	357314	ID=cds.MS.gene86977.t1;Parent=MS.gene86977.t1
chr2.3	exon	357803	358175	357803	ID=MS.gene86977.t1.exon2;Parent=MS.gene86977.t1
chr2.3	CDS	357803	358175	357803	ID=cds.MS.gene86977.t1;Parent=MS.gene86977.t1
chr2.3	exon	358460	358482	358460	ID=MS.gene86977.t1.exon3;Parent=MS.gene86977.t1
chr2.3	CDS	358460	358482	358460	ID=cds.MS.gene86977.t1;Parent=MS.gene86977.t1

Gene Sequence

>MS.gene86977

ATGGAAGAACATTTGTGTCACACAAACAGCACATTTGATGAAGAGTTCCTCAGAGATATCCTGTATCAGACTCCACAAGATCAATTCAGTGTCCCTATTGCCACAACTGGCCTAGTAAACAACTCATCCATCTATGTGTCACATTATTCAACTCAACATGCTGAAGAAAATCCAACCAACTCATTATCAATACCAACAATTGAACAACACCATGATTCTTTGCCTTTGTCATCATCAACGGATAACCAAAGGTCGAATTCAAAGAAGCCTCGAAGCGCTTCTGAGACATTAGATCACATAATGTCGGAGAGGAATAGGAGACAACTACTTACAAGTAAGATCATAGAACTTTCGGCCTTTATACCTGGATTGAAGAAGGTTAGTATATAATATATATTATATTAAACCTGGATGAATGAGTAAATTTTTATAGTAGTTATCATTGGATAGAATAGAAGCTACAATAAAAATTATCTAATGGCTGTGTAGATTGATAAGGTTCATGTGGTAACGGAAGCTATCAATTACGTGAAACGACTTGAAGAACGTTTGAAGGAGCTAGAAGAAGACGTTAAGAAGAAAGATGCAGGATCATTGAGCACCATAACAAGATCTCGTGTTCTAATTAACAAAGACACTGCAATCGGTGAAATGAACACTGAAGAATGGCGAAATGAATCACTTCTAGAGGTTGAAGCTAGGGTTCTTGGGAAGGAAGTGTTAATCAAGATTCATTGTGGGATGCAAGAAGAGATTGTGGTCAATATAATGTTGCAGCTTCAAATTCTTCATCTCTCCATAACAAGTACCAATGTCTTGCCATTTGGAAATACTCTTGACATCACCATTATTGCCCAGGCAAGTAATTACCAAAAAACTATAATATATATATAATTTCATGGAAGAAATTTAAGAAGCATGGTTCTAACTCAAGTTTGATTGTTATCCAAATTTATATATATATATATATACACAGATGGGTGATAAATACAAGTTGACCGTGAAGGAACCAGTGAAAAAACTAAGAGTAGTGGCTACGTTGCAGGCATGTGGTAATGTGCAACAATGAAGTGTGCATTTAGTATTTGGGATTTGAGAAGGTTCGATTAAAGCCATTGCTAATCAATTTGGATTTTTGGTGTGCAGGTTTAGGCTATGCTCTTGAATAA

Protein sequence

>MS.gene86977.t1

MEEHLCHTNSTFDEEFLRDILYQTPQDQFSVPIATTGLVNNSSIYVSHYSTQHAEENPTNSLSIPTIEQHHDSLPLSSSTDNQRSNSKKPRSASETLDHIMSERNRRQLLTSKIIELSAFIPGLKKIDKVHVVTEAINYVKRLEERLKELEEDVKKKDAGSLSTITRSRVLINKDTAIGEMNTEEWRNESLLEVEARVLGKEVLIKIHCGMQEEIVVNIMLQLQILHLSITSTNVLPFGNTLDITIIAQASLGYALE