AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene86980

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene86980.t1 MTR_2g104530 85.115 262 28 4 3 262 1 253 3.97e-153 428
MS.gene86980.t1 MTR_2g104500 64.158 279 53 11 3 262 1 251 2.14e-104 304
MS.gene86980.t1 MTR_2g104550 41.697 271 134 8 12 262 25 291 8.27e-53 174
MS.gene86980.t1 MTR_2g104490 40.942 276 133 9 8 262 21 287 9.00e-49 163
MS.gene86980.t1 MTR_2g104490 40.942 276 133 9 8 262 21 287 1.60e-48 163
MS.gene86980.t1 MTR_4g009540 44.292 219 110 5 48 262 100 310 1.01e-46 158
MS.gene86980.t1 MTR_4g009540 44.292 219 110 5 48 262 100 310 2.58e-46 157
MS.gene86980.t1 MTR_4g009110 40.183 219 123 3 48 262 115 329 6.92e-41 144
MS.gene86980.t1 MTR_8g009020 42.060 233 118 8 41 263 78 303 5.70e-39 139
MS.gene86980.t1 MTR_2g104560 40.719 167 88 4 99 262 46 204 4.62e-33 120
MS.gene86980.t1 MTR_8g069740 33.455 275 149 7 9 261 3 265 1.92e-31 117
MS.gene86980.t1 MTR_2g104590 42.683 164 73 3 99 262 61 203 2.20e-28 108
MS.gene86980.t1 MTR_5g014600 37.349 166 94 3 108 263 150 315 7.68e-28 109
MS.gene86980.t1 MTR_5g014520 39.634 164 94 2 100 262 150 309 1.02e-27 108
MS.gene86980.t1 MTR_6g047570 29.225 284 169 6 3 263 38 312 2.51e-27 108
MS.gene86980.t1 MTR_5g014640 33.668 199 117 3 74 263 112 304 3.59e-26 104
MS.gene86980.t1 MTR_7g080780 31.301 246 147 3 18 263 86 309 6.52e-25 101
MS.gene86980.t1 MTR_7g080780 31.301 246 147 3 18 263 73 296 6.66e-25 101
MS.gene86980.t1 MTR_8g009010 34.440 241 105 6 30 263 58 252 1.11e-24 100
MS.gene86980.t1 MTR_4g092700 32.189 233 146 6 33 263 89 311 1.35e-24 100
MS.gene86980.t1 MTR_4g097940 31.984 247 148 7 22 263 75 306 1.49e-24 100
MS.gene86980.t1 MTR_2g010450 35.032 157 98 2 108 263 178 331 8.41e-24 99.0
MS.gene86980.t1 MTR_7g451650 40.690 145 60 4 125 263 53 177 1.69e-23 94.7
MS.gene86980.t1 MTR_7g451650 40.136 147 62 4 123 263 51 177 1.73e-23 94.7
MS.gene86980.t1 MTR_7g050350 41.353 133 53 3 137 263 2 115 2.90e-23 92.4
MS.gene86980.t1 MTR_7g451650 41.353 133 53 3 137 263 2 115 2.90e-23 92.4
MS.gene86980.t1 MTR_4g009510 39.735 151 66 3 118 262 53 184 3.15e-23 94.4
MS.gene86980.t1 MTR_2g104650 33.333 258 156 5 9 262 3 248 8.19e-23 94.7
MS.gene86980.t1 MTR_0250s0040 35.669 157 96 3 108 263 89 241 3.06e-22 92.8
MS.gene86980.t1 MTR_0246s0020 32.447 188 116 5 84 262 169 354 4.50e-22 94.4
MS.gene86980.t1 MTR_4g097920 36.306 157 93 2 108 263 141 291 7.60e-22 92.8
MS.gene86980.t1 MTR_4g097920 36.306 157 93 2 108 263 156 306 9.76e-22 92.8
MS.gene86980.t1 MTR_0246s0020 32.447 188 116 5 84 262 169 354 9.91e-22 93.6
MS.gene86980.t1 MTR_0011s0260 34.615 156 97 3 108 262 184 335 2.49e-21 92.0
MS.gene86980.t1 MTR_5g014560 36.306 157 89 2 110 262 134 283 1.04e-20 89.7
MS.gene86980.t1 MTR_0011s0210 31.613 155 103 1 108 262 184 335 1.44e-20 90.1
MS.gene86980.t1 MTR_4g009420 36.986 146 70 3 118 263 54 177 3.19e-20 85.9
MS.gene86980.t1 MTR_4g066460 32.301 226 122 8 48 262 99 304 3.39e-20 88.6
MS.gene86980.t1 MTR_4g009420 36.986 146 70 3 118 263 54 177 5.48e-20 85.5
MS.gene86980.t1 MTR_4g097950 34.434 212 121 6 63 262 24 229 1.14e-19 85.9
MS.gene86980.t1 MTR_4g067010 33.548 155 97 3 108 262 148 296 1.35e-19 87.0
MS.gene86980.t1 MTR_0246s0050 29.268 164 104 2 100 262 144 296 2.60e-19 85.9
MS.gene86980.t1 MTR_4g098035 29.268 164 104 2 100 262 165 317 2.75e-19 86.3
MS.gene86980.t1 MTR_4g066380 32.258 155 98 1 108 262 154 301 2.52e-15 74.7
MS.gene86980.t1 MTR_5g014550 34.921 126 79 1 136 261 53 175 1.96e-13 67.8
MS.gene86980.t1 MTR_8g098275 26.437 174 105 3 108 258 309 482 6.34e-13 68.6
MS.gene86980.t1 MTR_8g098275 26.437 174 105 3 108 258 245 418 7.16e-13 68.2
MS.gene86980.t1 MTR_8g098275 26.437 174 105 3 108 258 402 575 7.17e-13 68.6
MS.gene86980.t1 MTR_1g072320 28.378 148 83 3 108 241 471 609 1.60e-11 64.3
MS.gene86980.t1 MTR_8g024790 27.753 227 144 6 29 246 213 428 1.65e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene86980.t1 AT2G22760 37.126 167 101 3 99 263 111 275 3.98e-27 106
MS.gene86980.t1 AT2G22760 37.349 166 100 3 99 262 111 274 4.28e-27 106
MS.gene86980.t1 AT4G37850 36.571 175 108 3 87 261 133 304 3.14e-25 102
MS.gene86980.t1 AT4G37850 36.571 175 108 3 87 261 133 304 3.14e-25 102
MS.gene86980.t1 AT2G22750 34.434 212 102 4 77 263 84 283 3.64e-22 93.6
MS.gene86980.t1 AT2G22750 34.434 212 102 4 77 263 84 283 5.06e-22 93.2
MS.gene86980.t1 AT2G22750 34.434 212 102 4 77 263 108 307 5.22e-22 93.6
MS.gene86980.t1 AT2G22750 34.434 212 102 4 77 263 112 311 5.38e-22 93.6
MS.gene86980.t1 AT2G22750 34.434 212 102 4 77 263 112 311 5.99e-22 93.6
MS.gene86980.t1 AT2G22770 32.738 168 101 2 108 263 133 300 1.08e-21 92.4
MS.gene86980.t1 AT4G09820 34.641 153 70 6 98 246 351 477 3.51e-14 72.4
MS.gene86980.t1 AT1G63650 28.571 168 97 2 91 239 389 552 1.28e-11 64.7
MS.gene86980.t1 AT1G63650 28.571 168 97 2 91 239 389 552 1.28e-11 64.7
MS.gene86980.t1 AT1G63650 28.571 168 97 2 91 239 389 552 1.28e-11 64.7

Find 51 sgRNAs with CRISPR-Local

Find 154 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCTTATTGTGAAGTATTAT+TGG 0.152976 2.3:+305320 None:intergenic
AGTATTAATCTTCTCTTCTT+TGG 0.221402 2.3:+306067 None:intergenic
ATCAATTGTGAGAAGGAAAA+TGG 0.286777 2.3:-305176 MS.gene86980:CDS
TGGCATTCAACTCAAAATAT+TGG 0.318480 2.3:-305156 MS.gene86980:CDS
GACACTTTCACTTTGATATC+AGG 0.319665 2.3:+305221 None:intergenic
ACCAATAATACTTCACAATA+AGG 0.330869 2.3:-305321 MS.gene86980:CDS
ACAGGATATGGATATGGATA+TGG 0.335606 2.3:-306348 None:intergenic
TGTTATACAGGATATGGATA+TGG 0.352119 2.3:-306354 None:intergenic
CCGTTTGGCAATTCAACTCT+TGG 0.391384 2.3:-305086 MS.gene86980:CDS
TGATTCAAAGCTTATATTTG+AGG 0.412613 2.3:+306112 None:intergenic
AGCACCAGCGTATTGCCGTT+TGG 0.418325 2.3:-305101 MS.gene86980:CDS
CTTCCCTGGTATTGTGGCTA+TGG 0.426831 2.3:+306169 None:intergenic
GCTGCTCCGGCTACTATAGA+TGG 0.433245 2.3:+306263 None:intergenic
ATATCAGGAAGCAATTCATT+TGG 0.433496 2.3:+305236 None:intergenic
CAAGGAATTCACCATCTTGA+TGG 0.441294 2.3:+306306 None:intergenic
CGTGGCAGCGGTGGCTGCTC+CGG 0.465276 2.3:+306250 None:intergenic
GACAAGGCCTCTATAGTTCA+AGG 0.488754 2.3:-305419 MS.gene86980:CDS
CGGCGAGCTTGCAATCTTCG+TGG 0.489491 2.3:+306232 None:intergenic
GCTTGCAATCTTCGTGGCAG+CGG 0.490965 2.3:+306238 None:intergenic
CATCAAGATGGTGAATTCCT+TGG 0.492158 2.3:-306305 MS.gene86980:CDS
TTTGGACTTCCCTGGTATTG+TGG 0.495026 2.3:+306163 None:intergenic
CACGAAGATTGCAAGCTCGC+CGG 0.503233 2.3:-306231 MS.gene86980:CDS
CACAATAAGGAAAATTATTG+TGG 0.503978 2.3:-305308 MS.gene86980:CDS
AATTCCATAGCCACAATACC+AGG 0.516400 2.3:-306173 MS.gene86980:CDS
TGGCATTACTATCATCGCTC+AGG 0.520862 2.3:-305066 MS.gene86980:intron
TGAATTCCTTGGCGACTACA+TGG 0.522050 2.3:-306294 MS.gene86980:CDS
ACACACTGGCAGAGAGGAGA+AGG 0.529594 2.3:-306019 MS.gene86980:CDS
GAAGAGAGAATGCTAGTGCT+AGG 0.534839 2.3:+306200 None:intergenic
TGCAATCTTCGTGGCAGCGG+TGG 0.540042 2.3:+306241 None:intergenic
ATATGGATATGGATATGGAT+TGG 0.543257 2.3:-306343 MS.gene86980:CDS
AAAGGTGGTTTGGACTTCCC+TGG 0.544519 2.3:+306155 None:intergenic
AAGATTCATTTCACTAGATG+TGG 0.551691 2.3:+305274 None:intergenic
GACAGCGCCTTGAACTATAG+AGG 0.553159 2.3:+305412 None:intergenic
AAACGGCAATACGCTGGTGC+TGG 0.555816 2.3:+305103 None:intergenic
CTAGTGCTAGGAAGCAGCTC+CGG 0.573968 2.3:+306212 None:intergenic
CCAAGAGTTGAATTGCCAAA+CGG 0.574130 2.3:+305086 None:intergenic
CAACAACCATCTATAGTAGC+CGG 0.584716 2.3:-306269 MS.gene86980:CDS
TATATTACCTTCGTTGAGCG+AGG 0.596335 2.3:+305951 None:intergenic
CATTTACATCAATTGTGAGA+AGG 0.601314 2.3:-305183 MS.gene86980:CDS
GAATCGATGTGCCATCAAGA+TGG 0.604112 2.3:-306317 MS.gene86980:CDS
GAATCAAAAGCAAATGACAA+AGG 0.608381 2.3:-306095 MS.gene86980:CDS
TATAATTCCTCGCTCAACGA+AGG 0.620367 2.3:-305958 MS.gene86980:intron
TTCATTTCACTAGATGTGGT+AGG 0.630513 2.3:+305278 None:intergenic
GAAGTACAACAAAACAAGAG+AGG 0.639060 2.3:-305350 MS.gene86980:CDS
AAAGACTCTATTTCACACAC+TGG 0.641330 2.3:-306033 MS.gene86980:CDS
GTTGTGCCATGTAGTCGCCA+AGG 0.642754 2.3:+306288 None:intergenic
TGATATCAAAGTGAAAGTGT+CGG 0.646805 2.3:-305219 MS.gene86980:CDS
ATTGCCAAACGGCAATACGC+TGG 0.648172 2.3:+305097 None:intergenic
TATTTCACACACTGGCAGAG+AGG 0.667005 2.3:-306025 MS.gene86980:CDS
TCAAGGCGCTGTCAATTACG+TGG 0.672473 2.3:-305402 MS.gene86980:CDS
ATTCCATAGCCACAATACCA+GGG 0.679504 2.3:-306172 MS.gene86980:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AACATGAAATTAAAATAAAA+TGG + chr2.3:306020-306039 None:intergenic 10.0%
!! ACATGAAATTAAAATAAAAT+GGG + chr2.3:306019-306038 None:intergenic 10.0%
!! TATATATATATATATAGACA+TGG - chr2.3:306252-306271 MS.gene86980:CDS 10.0%
!! AAATGTATTAATTAATGCAA+GGG + chr2.3:304201-304220 None:intergenic 15.0%
!! GAAAAAAAATCAAAGAAAAA+TGG + chr2.3:305498-305517 None:intergenic 15.0%
!!! TTATTTTAATTTCATGTTCT+GGG - chr2.3:306022-306041 MS.gene86980:CDS 15.0%
!!! TTTATTTTAATTTCATGTTC+TGG - chr2.3:306021-306040 MS.gene86980:CDS 15.0%
!! AATGTATTAATTAATGCAAG+GGG + chr2.3:304200-304219 None:intergenic 20.0%
!! GAAATGTATTAATTAATGCA+AGG + chr2.3:304202-304221 None:intergenic 20.0%
!! TAATAAGATTGTGTATCTAA+TGG - chr2.3:306127-306146 MS.gene86980:CDS 20.0%
!! TTTCCCTAAAAAGTTAATAT+AGG + chr2.3:304380-304399 None:intergenic 20.0%
!!! ACTCCTATATTAACTTTTTA+GGG - chr2.3:304374-304393 MS.gene86980:intron 20.0%
!!! TACTCCTATATTAACTTTTT+AGG - chr2.3:304373-304392 MS.gene86980:intron 20.0%
!!! TATTTTGTTATAAAGAGAGT+GGG + chr2.3:305579-305598 None:intergenic 20.0%
! AAAACTTCAAAAAAGAGTGA+TGG - chr2.3:304716-304735 MS.gene86980:intron 25.0%
! AAAGAGTTTGGTATTTCATT+AGG + chr2.3:305523-305542 None:intergenic 25.0%
! AAGATTCATTGAATCATAGA+TGG + chr2.3:304489-304508 None:intergenic 25.0%
! ACCAATAATACTTCACAATA+AGG - chr2.3:304773-304792 MS.gene86980:intron 25.0%
! AGAAAGTAATTTCACAGAAT+TGG + chr2.3:305602-305621 None:intergenic 25.0%
! AGTATTAATCTTCTCTTCTT+TGG + chr2.3:304030-304049 None:intergenic 25.0%
! ATGTATTAATTAATGCAAGG+GGG + chr2.3:304199-304218 None:intergenic 25.0%
! CACAATAAGGAAAATTATTG+TGG - chr2.3:304786-304805 MS.gene86980:intron 25.0%
! GAAGAATAAGAAGATGATTA+AGG + chr2.3:305555-305574 None:intergenic 25.0%
! TGATTCAAAGCTTATATTTG+AGG + chr2.3:303985-304004 None:intergenic 25.0%
! TTTAATAGTCATGATACGAT+AGG - chr2.3:305205-305224 MS.gene86980:CDS 25.0%
! TTTGATGTAGTGTTAAAGTA+GGG + chr2.3:305076-305095 None:intergenic 25.0%
!! CAAAAAAATTCTAGTGTTGA+AGG - chr2.3:306100-306119 MS.gene86980:CDS 25.0%
!! TCCTTATTGTGAAGTATTAT+TGG + chr2.3:304777-304796 None:intergenic 25.0%
!! TTTTGATGTAGTGTTAAAGT+AGG + chr2.3:305077-305096 None:intergenic 25.0%
!!! CTTTGAGAATTTTTTTCAGT+GGG - chr2.3:305809-305828 MS.gene86980:intron 25.0%
!!! GGGAAAAAAAGAGAATTTTT+TGG - chr2.3:304394-304413 MS.gene86980:intron 25.0%
!!! GTATTTTGTTATAAAGAGAG+TGG + chr2.3:305580-305599 None:intergenic 25.0%
!!! TAACATGGTTTTACTTGATT+TGG + chr2.3:305457-305476 None:intergenic 25.0%
!!! TCTTTGAGAATTTTTTTCAG+TGG - chr2.3:305808-305827 MS.gene86980:intron 25.0%
!!! TGTTATTGACTCGAATTTAA+TGG - chr2.3:304620-304639 MS.gene86980:intron 25.0%
AAAATAAAATGGGTGCTTGA+CGG + chr2.3:306009-306028 None:intergenic 30.0%
AACATTAACTTTCACATGGT+GGG + chr2.3:305266-305285 None:intergenic 30.0%
AAGATTCATTTCACTAGATG+TGG + chr2.3:304823-304842 None:intergenic 30.0%
AATCTATGTGGAATTTAGCA+TGG + chr2.3:306194-306213 None:intergenic 30.0%
AGGAATTGAAGAAAAGAGTT+TGG + chr2.3:305535-305554 None:intergenic 30.0%
ATATCAGGAAGCAATTCATT+TGG + chr2.3:304861-304880 None:intergenic 30.0%
ATCAATTGTGAGAAGGAAAA+TGG - chr2.3:304918-304937 MS.gene86980:intron 30.0%
ATCTATGTGGAATTTAGCAT+GGG + chr2.3:306193-306212 None:intergenic 30.0%
CATTTACATCAATTGTGAGA+AGG - chr2.3:304911-304930 MS.gene86980:intron 30.0%
CTATCTTACACTAATCTATG+TGG + chr2.3:306206-306225 None:intergenic 30.0%
GAAAATTAGATCCAGAAAAC+AGG - chr2.3:305653-305672 MS.gene86980:intron 30.0%
GAATCAAAAGCAAATGACAA+AGG - chr2.3:303999-304018 MS.gene86980:intron 30.0%
TAACACCTGATAACAAAGAA+TGG - chr2.3:305853-305872 MS.gene86980:intron 30.0%
TAACATTAACTTTCACATGG+TGG + chr2.3:305267-305286 None:intergenic 30.0%
TGGCATTCAACTCAAAATAT+TGG - chr2.3:304938-304957 MS.gene86980:intron 30.0%
TGTATTAATTAATGCAAGGG+GGG + chr2.3:304198-304217 None:intergenic 30.0%
TTAACTCATGAGTTAGTATG+TGG + chr2.3:304590-304609 None:intergenic 30.0%
TTAATACACAATCCAGATCT+CGG - chr2.3:304507-304526 MS.gene86980:intron 30.0%
TTCATAACATGTTCGTAACA+TGG + chr2.3:305472-305491 None:intergenic 30.0%
TTCATTGAATCATAGATGGA+CGG + chr2.3:304485-304504 None:intergenic 30.0%
TTCGAGTCAATAACACAATT+TGG + chr2.3:304615-304634 None:intergenic 30.0%
! TGATATCAAAGTGAAAGTGT+CGG - chr2.3:304875-304894 MS.gene86980:intron 30.0%
! TTTCTCCATTCTTTGTTATC+AGG + chr2.3:305861-305880 None:intergenic 30.0%
!!! AATTTTTTTCAGTGGGCAAA+TGG - chr2.3:305816-305835 MS.gene86980:intron 30.0%
!!! TTTTGAAGCTTTGAGATGAA+TGG + chr2.3:305729-305748 None:intergenic 30.0%
AAAGACTCTATTTCACACAC+TGG - chr2.3:304061-304080 MS.gene86980:intron 35.0%
AATTGTGGCTTCATGTTCTT+AGG - chr2.3:305925-305944 MS.gene86980:intron 35.0%
ATAGTCATGATACGATAGGA+TGG - chr2.3:305209-305228 MS.gene86980:CDS 35.0%
ATTGTGGCTTCATGTTCTTA+GGG - chr2.3:305926-305945 MS.gene86980:intron 35.0%
GAAGTACAACAAAACAAGAG+AGG - chr2.3:304744-304763 MS.gene86980:intron 35.0%
GACACTTTCACTTTGATATC+AGG + chr2.3:304876-304895 None:intergenic 35.0%
GGCTAACATTAACTTTCACA+TGG + chr2.3:305270-305289 None:intergenic 35.0%
GTGTTCTTTACTTGATCTGA+TGG + chr2.3:305291-305310 None:intergenic 35.0%
TATCCAGATCCACTGAAAAT+GGG - chr2.3:305778-305797 MS.gene86980:intron 35.0%
TGGATAGGTTGTAGTTTATG+TGG + chr2.3:305764-305783 None:intergenic 35.0%
TTCATTTCACTAGATGTGGT+AGG + chr2.3:304819-304838 None:intergenic 35.0%
! ACAGAAGCGATGATGAATTT+TGG + chr2.3:305694-305713 None:intergenic 35.0%
! AGTTGGGACAAAGATTTTGA+TGG + chr2.3:305407-305426 None:intergenic 35.0%
! CACCTTTTTACCCTAAAAAG+AGG - chr2.3:303955-303974 MS.gene86980:intron 35.0%
! CAGAAGCGATGATGAATTTT+GGG + chr2.3:305693-305712 None:intergenic 35.0%
! GAATTTATCCCCATTTTCAG+TGG + chr2.3:305790-305809 None:intergenic 35.0%
! TTGAGGAAGTCCTCTTTTTA+GGG + chr2.3:303968-303987 None:intergenic 35.0%
! TTTAGGGTAAAAAGGTGGTT+TGG + chr2.3:303952-303971 None:intergenic 35.0%
! TTTGAGGAAGTCCTCTTTTT+AGG + chr2.3:303969-303988 None:intergenic 35.0%
!! AACATTGACTTTCTCTTGGT+GGG - chr2.3:306152-306171 MS.gene86980:CDS 35.0%
!! GGATTTGATTTTGCTGTGAA+GGG + chr2.3:305436-305455 None:intergenic 35.0%
!! TAACATTGACTTTCTCTTGG+TGG - chr2.3:306151-306170 MS.gene86980:CDS 35.0%
!! TGGATTTGATTTTGCTGTGA+AGG + chr2.3:305437-305456 None:intergenic 35.0%
!!! CTCTTTTTAGGGTAAAAAGG+TGG + chr2.3:303957-303976 None:intergenic 35.0%
!!! GTCCTCTTTTTAGGGTAAAA+AGG + chr2.3:303960-303979 None:intergenic 35.0%
AATTCCATAGCCACAATACC+AGG - chr2.3:303921-303940 MS.gene86980:intron 40.0%
ATCCAGATCCACTGAAAATG+GGG - chr2.3:305779-305798 MS.gene86980:intron 40.0%
ATTCCATAGCCACAATACCA+GGG - chr2.3:303922-303941 MS.gene86980:intron 40.0%
CAACAACCATCTATAGTAGC+CGG - chr2.3:303825-303844 MS.gene86980:intron 40.0%
CAAGGAATTCACCATCTTGA+TGG + chr2.3:303791-303810 None:intergenic 40.0%
CATCAAGATGGTGAATTCCT+TGG - chr2.3:303789-303808 MS.gene86980:intron 40.0%
CCAAGAGTTGAATTGCCAAA+CGG + chr2.3:305011-305030 None:intergenic 40.0%
CTATCCAGATCCACTGAAAA+TGG - chr2.3:305777-305796 MS.gene86980:intron 40.0%
TATAATTCCTCGCTCAACGA+AGG - chr2.3:304136-304155 MS.gene86980:intron 40.0%
TATATTACCTTCGTTGAGCG+AGG + chr2.3:304146-304165 None:intergenic 40.0%
TCATGATACGATAGGATGGT+GGG - chr2.3:305213-305232 MS.gene86980:CDS 40.0%
TTAGTATGTGGACGCAGTTA+CGG + chr2.3:304578-304597 None:intergenic 40.0%
! CATTTTCAGTGGATCTGGAT+AGG + chr2.3:305779-305798 None:intergenic 40.0%
!! AGGATGGTGGGATTTAGTAT+GGG - chr2.3:305225-305244 MS.gene86980:CDS 40.0%
!! GAAGCTTTGAGATGAATGGA+AGG + chr2.3:305725-305744 None:intergenic 40.0%
!! GGCTAACATTGACTTTCTCT+TGG - chr2.3:306148-306167 MS.gene86980:CDS 40.0%
!! TAGGATGGTGGGATTTAGTA+TGG - chr2.3:305224-305243 MS.gene86980:CDS 40.0%
!! TCTGAAAGTGTTGAGAGAGA+TGG - chr2.3:306061-306080 MS.gene86980:CDS 40.0%
!! TTGATTTTGCTGTGAAGGGA+AGG + chr2.3:305432-305451 None:intergenic 40.0%
!!! GATTTTGATGGTGTTGTTGC+TGG + chr2.3:305395-305414 None:intergenic 40.0%
CCGTTTGGCAATTCAACTCT+TGG - chr2.3:305008-305027 MS.gene86980:intron 45.0%
GAAGAGAGAATGCTAGTGCT+AGG + chr2.3:303897-303916 None:intergenic 45.0%
GAATCGATGTGCCATCAAGA+TGG - chr2.3:303777-303796 MS.gene86980:intron 45.0%
GACAAGGCCTCTATAGTTCA+AGG - chr2.3:304675-304694 MS.gene86980:intron 45.0%
GGTGGGATTTAGTATGGGAA+AGG - chr2.3:305230-305249 MS.gene86980:CDS 45.0%
GTCATGATACGATAGGATGG+TGG - chr2.3:305212-305231 MS.gene86980:CDS 45.0%
TATTTCACACACTGGCAGAG+AGG - chr2.3:304069-304088 MS.gene86980:intron 45.0%
TGAATTCCTTGGCGACTACA+TGG - chr2.3:303800-303819 MS.gene86980:intron 45.0%
TGGCATTACTATCATCGCTC+AGG - chr2.3:305028-305047 MS.gene86980:intron 45.0%
TGTGTGAAACTACCGAGATC+TGG + chr2.3:304522-304541 None:intergenic 45.0%
TTGGTATGAAGGAGTGATGG+TGG + chr2.3:305363-305382 None:intergenic 45.0%
TTTGGACTTCCCTGGTATTG+TGG + chr2.3:303934-303953 None:intergenic 45.0%
! ATCCCCATTTTCAGTGGATC+TGG + chr2.3:305784-305803 None:intergenic 45.0%
! TCCTTTTCGACAGACAGACA+AGG - chr2.3:304659-304678 MS.gene86980:intron 45.0%
! TGGTTGGTATGAAGGAGTGA+TGG + chr2.3:305366-305385 None:intergenic 45.0%
!! AGTGATGGTGGTGGAAGATA+GGG + chr2.3:305351-305370 None:intergenic 45.0%
!! GAAAGTGTTGAGAGAGATGG+TGG - chr2.3:306064-306083 MS.gene86980:CDS 45.0%
!! GCATCTAGCTTCCTGTTTTC+TGG + chr2.3:305667-305686 None:intergenic 45.0%
!! TTGATGGTGTTGTTGCTGGT+GGG + chr2.3:305391-305410 None:intergenic 45.0%
!! TTTGATGGTGTTGTTGCTGG+TGG + chr2.3:305392-305411 None:intergenic 45.0%
!! ATTAAAATAAAATAAAATTG+TGG - chr2.3:305910-305929 MS.gene86980:intron 5.0%
!!! AATTTTCAATAAAAATAAAA+AGG + chr2.3:305640-305659 None:intergenic 5.0%
ATTGCCAAACGGCAATACGC+TGG + chr2.3:305000-305019 None:intergenic 50.0%
CTGTGAAGGGAAGGAGAGTT+GGG + chr2.3:305423-305442 None:intergenic 50.0%
CTTCCCTGGTATTGTGGCTA+TGG + chr2.3:303928-303947 None:intergenic 50.0%
GACAGCGCCTTGAACTATAG+AGG + chr2.3:304685-304704 None:intergenic 50.0%
GCCTTGTCTGTCTGTCGAAA+AGG + chr2.3:304663-304682 None:intergenic 50.0%
! AAAGGTGGTTTGGACTTCCC+TGG + chr2.3:303942-303961 None:intergenic 50.0%
! AGTGTTGAGAGAGATGGTGG+TGG - chr2.3:306067-306086 MS.gene86980:CDS 50.0%
!! AGAGAGATGGTGGTGGAAGA+TGG - chr2.3:306074-306093 MS.gene86980:CDS 50.0%
!! GAGTGATGGTGGTGGAAGAT+AGG + chr2.3:305352-305371 None:intergenic 50.0%
!! GTATGAAGGAGTGATGGTGG+TGG + chr2.3:305360-305379 None:intergenic 50.0%
!! TCAAGGCGCTGTCAATTACG+TGG - chr2.3:304692-304711 MS.gene86980:intron 50.0%
!! TGATGGTGTTGTTGCTGGTG+GGG + chr2.3:305390-305409 None:intergenic 50.0%
AAACGGCAATACGCTGGTGC+TGG + chr2.3:304994-305013 None:intergenic 55.0%
ACACACTGGCAGAGAGGAGA+AGG - chr2.3:304075-304094 MS.gene86980:intron 55.0%
AGCACCAGCGTATTGCCGTT+TGG - chr2.3:304993-305012 MS.gene86980:intron 55.0%
CACGAAGATTGCAAGCTCGC+CGG - chr2.3:303863-303882 MS.gene86980:intron 55.0%
CTAGTGCTAGGAAGCAGCTC+CGG + chr2.3:303885-303904 None:intergenic 55.0%
GCTGCTCCGGCTACTATAGA+TGG + chr2.3:303834-303853 None:intergenic 55.0%
GCTGTGAAGGGAAGGAGAGT+TGG + chr2.3:305424-305443 None:intergenic 55.0%
GCTTGCAATCTTCGTGGCAG+CGG + chr2.3:303859-303878 None:intergenic 55.0%
GTTGTGCCATGTAGTCGCCA+AGG + chr2.3:303809-303828 None:intergenic 55.0%
! TTGTTGCTGGTGGGGTTGGT+TGG + chr2.3:305382-305401 None:intergenic 55.0%
!! GGTGGGGTTGGTTGGTATGA+AGG + chr2.3:305374-305393 None:intergenic 55.0%
CGGCGAGCTTGCAATCTTCG+TGG + chr2.3:303865-303884 None:intergenic 60.0%
TGCAATCTTCGTGGCAGCGG+TGG + chr2.3:303856-303875 None:intergenic 60.0%
!! GGTGTTGTTGCTGGTGGGGT+TGG + chr2.3:305386-305405 None:intergenic 60.0%
CGTGGCAGCGGTGGCTGCTC+CGG + chr2.3:303847-303866 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 303753 306363 303753 ID=MS.gene86980
chr2.3 mRNA 303753 306363 303753 ID=MS.gene86980.t1;Parent=MS.gene86980
chr2.3 exon 305959 306363 305959 ID=MS.gene86980.t1.exon1;Parent=MS.gene86980.t1
chr2.3 CDS 305959 306363 305959 ID=cds.MS.gene86980.t1;Parent=MS.gene86980.t1
chr2.3 exon 305067 305444 305067 ID=MS.gene86980.t1.exon2;Parent=MS.gene86980.t1
chr2.3 CDS 305067 305444 305067 ID=cds.MS.gene86980.t1;Parent=MS.gene86980.t1
chr2.3 exon 303753 303761 303753 ID=MS.gene86980.t1.exon3;Parent=MS.gene86980.t1
chr2.3 CDS 303753 303761 303753 ID=cds.MS.gene86980.t1;Parent=MS.gene86980.t1
Gene Sequence

>MS.gene86980

ATGGATATGGATATGGATTGGCTAGAATCGATGTGCCATCAAGATGGTGAATTCCTTGGCGACTACATGGCACAACAACCATCTATAGTAGCCGGAGCAGCCACCGCTGCCACGAAGATTGCAAGCTCGCCGGAGCTGCTTCCTAGCACTAGCATTCTCTCTTCTAATAATTCCATAGCCACAATACCAGGGAAGTCCAAACCACCTTTTTACCCTAAAAAGAGGACTTCCTCAAATATAAGCTTTGAATCAAAAGCAAATGACAAAGGATTAGCCAAAGAAGAGAAGATTAATACTAGAAGCAAATCAAAGACTCTATTTCACACACTGGCAGAGAGGAGAAGGAGACTTGAACTCGCACATAAATTCACAGAGCTTTCATCTATAATTCCTCGCTCAACGAAGGTAATATACTCAACAAATTATTTAGACTCAATTTTTACCCCCCTTGCATTAATTAATACATTTCAGTGCTTTGATTTGAATTACATACATACTGAATCAAATCAAAACAAATGATGTAATAAAACAAATCAAAACAAAACAAATGATGTAATAAAACAAATCAAAACAAAGCATTCATCATAGTTTTTTTATATTATTTATTTTGAATATAAATATACTCCTATATTAACTTTTTAGGGAAAAAAAGAGAATTTTTTGGAAAATATGTTAAAAACTTATAGTGAACGAATTCACAAAATTCAAAGTAGTAGTAATAAATCTCGACCGTCCATCTATGATTCAATGAATCTTAATACACAATCCAGATCTCGGTAGTTTCACACAATGTGAAATCTCAATCGTTCTTTTACAATCAGACCGTAACTGCGTCCACATACTAACTCATGAGTTAAATCCAAATTGTGTTATTGACTCGAATTTAATGGTTTCTGCATATGTATTTCCTTTTCGACAGACAGACAAGGCCTCTATAGTTCAAGGCGCTGTCAATTACGTGGAAAAACTTCAAAAAAGAGTGATGGAGTTAGAAGTACAACAAAACAAGAGAGGCAAAGAACCAATAATACTTCACAATAAGGAAAATTATTGTGGAGTTCGACCTACCACATCTAGTGAAATGAATCTTGACAATTATCTTAGACCAAATGAATTGCTTCCTGATATCAAAGTGAAAGTGTCGGAGAATAATATACTCATTTACATCAATTGTGAGAAGGAAAATGGCATTCAACTCAAAATATTGGACATGCTTCAAAATCTTCATTTGTTTGTCACCAGCACCAGCGTATTGCCGTTTGGCAATTCAACTCTTGGCATTACTATCATCGCTCAGGTATGCATCTCTAAACTTTAATGCCCTACTTTAACACTACATCAAAACTAATTATGAACATTTTCTTTGACAAATTGTACTAATTATAAACATGCATGAGAAATGTAGTTTTTGCTATAACTTTTATAAAAATAAAAAATAAAATAAAACATTTGTTTAATAGTCATGATACGATAGGATGGTGGGATTTAGTATGGGAAAGGAGAGTTGTTTCCCACCATGTGAAAGTTAATGTTAGCCATCAGATCAAGTAAAGAACACAATCTTATCATACACTCTCAACACTAGATTATTTTGTCCCTATCTTCCACCACCATCACTCCTTCATACCAACCAACCCCACCAGCAACAACACCATCAAAATCTTTGTCCCAACTCTCCTTCCCTTCACAGCAAAATCAAATCCAAATCAAGTAAAACCATGTTACGAACATGTTATGAACAACCATTTTTCTTTGATTTTTTTTCTTCCTAATGAAATACCAAACTCTTTTCTTCAATTCCTTAATCATCTTCTTATTCTTCACCCACTCTCTTTATAACAAAATACCAATTCTGTGAAATTACTTTCTTTCTCTTTGTTTTATCCTTTTTATTTTTATTGAAAATTAGATCCAGAAAACAGGAAGCTAGATGCAGTCCCAAAATTCATCATCGCTTCTGTCTTTAACTCCTTCCATTCATCTCAAAGCTTCAAAATTTCAAACACAACCACATAAACTACAACCTATCCAGATCCACTGAAAATGGGGATAAATTCTTTGAGAATTTTTTTCAGTGGGCAAATGGTGAAAGAAATAAAATAACACCTGATAACAAAGAATGGAGAAAAAAATTCAATAATTGAACAAATCATAGACATTAAAATAAAATAAAATTGTGGCTTCATGTTCTTAGGGCAACACATGATGTGAAATTGAAAGAAGAAAAAAAAACTGCGAATTCATGCTGTGATACCGTCAAGCACCCATTTTATTTTAATTTCATGTTCTGGGTTATTAAGTTTTGATTTCTGAAAGTGTTGAGAGAGATGGTGGTGGAAGATGGTGACAAAAAAATTCTAGTGTTGAAGGTGTATAATAAGATTGTGTATCTAATGGCTAACATTGACTTTCTCTTGGTGGGAAACAACTATGTTTTCCCATGCTAAATTCCACATAGATTAGTGTAAGATAGAATATAAAAATAGATATAATATGATATATATATATATATATAGACATGGTAAGTCTAATCGTATCTTATGTTTGATGTACAATAAAAATAAACTTAAATATTATATATTATTTTATTTGTAGATTTTAGATAAACTAA
Protein sequence

>MS.gene86980.t1

MDMDMDWLESMCHQDGEFLGDYMAQQPSIVAGAATAATKIASSPELLPSTSILSSNNSIATIPGKSKPPFYPKKRTSSNISFESKANDKGLAKEEKINTRSKSKTLFHTLAERRRRLELAHKFTELSSIIPRSTKTDKASIVQGAVNYVEKLQKRVMELEVQQNKRGKEPIILHNKENYCGVRPTTSSEMNLDNYLRPNELLPDIKVKVSENNILIYINCEKENGIQLKILDMLQNLHLFVTSTSVLPFGNSTLGITIIAQIN