Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87069.t1 | XP_013449239.1 | 86.5 | 349 | 37 | 1 | 1 | 339 | 1 | 349 | 2.00E-126 | 462.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87069.t1 | F4HNU8 | 28.5 | 355 | 192 | 9 | 1 | 338 | 1 | 310 | 1.1e-24 | 115.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87069.t1 | A0A072U1F0 | 86.5 | 349 | 37 | 1 | 1 | 339 | 1 | 349 | 1.5e-126 | 462.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene87069.t1 | TF | OFP |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87069.t1 | MTR_7g072630 | 92.550 | 349 | 16 | 1 | 1 | 339 | 1 | 349 | 0.0 | 616 |
| MS.gene87069.t1 | MTR_7g072875 | 91.039 | 279 | 22 | 1 | 61 | 339 | 12 | 287 | 2.22e-180 | 500 |
| MS.gene87069.t1 | MTR_4g028930 | 49.631 | 407 | 108 | 16 | 1 | 337 | 1 | 380 | 1.07e-91 | 279 |
| MS.gene87069.t1 | MTR_5g075210 | 40.437 | 366 | 167 | 12 | 1 | 336 | 1 | 345 | 3.32e-61 | 199 |
| MS.gene87069.t1 | MTR_2g018030 | 60.606 | 66 | 26 | 0 | 271 | 336 | 277 | 342 | 3.14e-25 | 104 |
| MS.gene87069.t1 | MTR_8g087530 | 57.576 | 66 | 28 | 0 | 271 | 336 | 258 | 323 | 3.57e-22 | 95.9 |
| MS.gene87069.t1 | MTR_8g103520 | 53.226 | 62 | 29 | 0 | 271 | 332 | 224 | 285 | 2.15e-16 | 79.0 |
| MS.gene87069.t1 | MTR_0907s0020 | 44.928 | 69 | 38 | 0 | 264 | 332 | 95 | 163 | 1.48e-14 | 71.2 |
| MS.gene87069.t1 | MTR_4g052040 | 45.070 | 71 | 39 | 0 | 262 | 332 | 90 | 160 | 7.73e-14 | 69.3 |
| MS.gene87069.t1 | MTR_4g132740 | 47.619 | 63 | 33 | 0 | 273 | 335 | 83 | 145 | 3.74e-13 | 66.6 |
| MS.gene87069.t1 | MTR_3g111780 | 42.500 | 80 | 46 | 0 | 253 | 332 | 180 | 259 | 1.74e-12 | 67.4 |
| MS.gene87069.t1 | MTR_7g105790 | 44.068 | 59 | 33 | 0 | 274 | 332 | 113 | 171 | 3.21e-12 | 65.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87069.t1 | AT2G30400 | 33.243 | 370 | 160 | 11 | 1 | 336 | 1 | 317 | 8.70e-40 | 143 |
| MS.gene87069.t1 | AT1G06920 | 33.333 | 369 | 154 | 15 | 1 | 337 | 1 | 309 | 9.87e-40 | 142 |
| MS.gene87069.t1 | AT5G58360 | 30.791 | 354 | 158 | 11 | 1 | 336 | 5 | 289 | 1.07e-33 | 126 |
| MS.gene87069.t1 | AT5G01840 | 48.800 | 125 | 48 | 3 | 214 | 335 | 151 | 262 | 1.26e-25 | 103 |
| MS.gene87069.t1 | AT4G18830 | 39.450 | 109 | 58 | 1 | 235 | 335 | 238 | 346 | 2.13e-19 | 88.2 |
| MS.gene87069.t1 | AT5G19650 | 50.000 | 66 | 33 | 0 | 270 | 335 | 153 | 218 | 1.05e-15 | 75.5 |
| MS.gene87069.t1 | AT3G52525 | 46.032 | 63 | 34 | 0 | 272 | 334 | 67 | 129 | 2.40e-13 | 67.4 |
| MS.gene87069.t1 | AT2G18500 | 41.791 | 67 | 39 | 0 | 269 | 335 | 224 | 290 | 4.50e-13 | 69.3 |
| MS.gene87069.t1 | AT2G36026 | 48.214 | 56 | 29 | 0 | 277 | 332 | 92 | 147 | 5.94e-12 | 63.9 |
Find 42 sgRNAs with CRISPR-Local
Find 59 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACACGGTCTGTTCTGATTTC+TGG | 0.132362 | 7.4:+31901137 | None:intergenic |
| TCAAAGTGTTCAAGCAAATA+TGG | 0.233458 | 7.4:-31900527 | MS.gene87069:CDS |
| AATGATGAACACAACGTTAA+AGG | 0.235673 | 7.4:-31900912 | MS.gene87069:CDS |
| TTCTTCCTTTGTTGTGTTGT+TGG | 0.243928 | 7.4:+31901410 | None:intergenic |
| AGAATTGTTCTACTGATTAT+CGG | 0.312127 | 7.4:+31900494 | None:intergenic |
| CATTAGAACATCAAAGGATT+TGG | 0.325611 | 7.4:-31900607 | MS.gene87069:CDS |
| TGAAGATTCTATTGCTTGTT+TGG | 0.331737 | 7.4:+31901288 | None:intergenic |
| TTTGTTGTGTTGTTGGTGTT+TGG | 0.367246 | 7.4:+31901417 | None:intergenic |
| ATGATGAACACAACGTTAAA+GGG | 0.389606 | 7.4:-31900911 | MS.gene87069:CDS |
| TACGTCTTCCACCATCGTTT+CGG | 0.400519 | 7.4:-31900736 | MS.gene87069:CDS |
| CCATCGTTTCGGCTGGCAAC+CGG | 0.420330 | 7.4:-31900725 | MS.gene87069:CDS |
| GTTCACCAGGTGTGAAACTT+AGG | 0.443025 | 7.4:-31900812 | MS.gene87069:CDS |
| TCTGTTCTGATTTCTGGCTC+AGG | 0.447190 | 7.4:+31901143 | None:intergenic |
| TCTGGTTTGGTCACAATTGG+TGG | 0.452293 | 7.4:+31901002 | None:intergenic |
| TGGCAACCGGAGAAGTCTTT+CGG | 0.457447 | 7.4:-31900712 | MS.gene87069:CDS |
| TTTCTTGGATTGCTTTGGTA+GGG | 0.457667 | 7.4:+31901338 | None:intergenic |
| GGAGATGATGATGAAGTCTT+TGG | 0.474574 | 7.4:+31901209 | None:intergenic |
| CAGACATGATGCCTAATGCT+TGG | 0.484513 | 7.4:-31901475 | MS.gene87069:CDS |
| TTTGATGATGATATTCTTGG+TGG | 0.485239 | 7.4:+31901257 | None:intergenic |
| CTGTTCTGATTTCTGGCTCA+GGG | 0.485970 | 7.4:+31901144 | None:intergenic |
| AGCTTGTAAAACCAAGCATT+AGG | 0.493200 | 7.4:+31901464 | None:intergenic |
| AGATTCTCTGTTAGTTCACC+AGG | 0.512695 | 7.4:-31900825 | MS.gene87069:CDS |
| AGGAGATTTCAGAGAATCAA+TGG | 0.515867 | 7.4:-31900652 | MS.gene87069:CDS |
| ATTCACCAAGAATTTCAAAC+CGG | 0.519789 | 7.4:-31900785 | MS.gene87069:CDS |
| TCTTCCACCATCGTTTCGGC+TGG | 0.529072 | 7.4:-31900732 | MS.gene87069:CDS |
| CTATCAAGTGGAGAATCAAA+TGG | 0.529685 | 7.4:+31901188 | None:intergenic |
| ACTCATCTGAATTCAAAGAA+AGG | 0.530280 | 7.4:+31900565 | None:intergenic |
| CTAACAGAGAATCTTCTACT+TGG | 0.532521 | 7.4:+31900834 | None:intergenic |
| GAACAACATTAGAACATCAA+AGG | 0.551741 | 7.4:-31900613 | MS.gene87069:CDS |
| GGAAAATTCAAACACATGGC+CGG | 0.552191 | 7.4:-31900764 | MS.gene87069:CDS |
| AACTATCCGAAAGACTTCTC+CGG | 0.559644 | 7.4:+31900706 | None:intergenic |
| AAACACCAACAACACAACAA+AGG | 0.563217 | 7.4:-31901415 | MS.gene87069:CDS |
| TCGATTATGATGTCGTTCGT+CGG | 0.569783 | 7.4:+31901107 | None:intergenic |
| GTTGCCAGCCGAAACGATGG+TGG | 0.586748 | 7.4:+31900728 | None:intergenic |
| GATTCTCTGAAATCTCCTTG+TGG | 0.590560 | 7.4:+31900657 | None:intergenic |
| CCGGTTGCCAGCCGAAACGA+TGG | 0.591822 | 7.4:+31900725 | None:intergenic |
| AACCGGAAAATTCAAACACA+TGG | 0.611341 | 7.4:-31900768 | MS.gene87069:CDS |
| GATTCTGCAGAGCTATCAAG+TGG | 0.613063 | 7.4:+31901176 | None:intergenic |
| TTGATCCTAAGTTTCACACC+TGG | 0.631177 | 7.4:+31900807 | None:intergenic |
| AGATTTCAGAGAATCAATGG+TGG | 0.652480 | 7.4:-31900649 | MS.gene87069:CDS |
| AAGTCATCATTGAATCCACA+AGG | 0.674680 | 7.4:-31900672 | MS.gene87069:CDS |
| CGTTCGTCGGAAGAAGAACA+CGG | 0.706093 | 7.4:+31901120 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACTAAAAGTCAAGATTTTGA+AGG | - | chr7.4:31901117-31901136 | MS.gene87069:CDS | 25.0% |
| !! | ATTTGATGAATTTTTGCTTC+TGG | + | chr7.4:31901074-31901093 | None:intergenic | 25.0% |
| !! | GTAAAGTAGTAAGATTTTCT+TGG | + | chr7.4:31900683-31900702 | None:intergenic | 25.0% |
| !! | TTTGATGAATTTTTGCTTCT+GGG | + | chr7.4:31901073-31901092 | None:intergenic | 25.0% |
| !!! | TGTTTTGATGATGATATTCT+TGG | + | chr7.4:31900752-31900771 | None:intergenic | 25.0% |
| AATGATGAACACAACGTTAA+AGG | - | chr7.4:31901091-31901110 | MS.gene87069:CDS | 30.0% | |
| ACTCATCTGAATTCAAAGAA+AGG | + | chr7.4:31901441-31901460 | None:intergenic | 30.0% | |
| ATGATGAACACAACGTTAAA+GGG | - | chr7.4:31901092-31901111 | MS.gene87069:CDS | 30.0% | |
| ATTCACCAAGAATTTCAAAC+CGG | - | chr7.4:31901218-31901237 | MS.gene87069:CDS | 30.0% | |
| CATTAGAACATCAAAGGATT+TGG | - | chr7.4:31901396-31901415 | MS.gene87069:CDS | 30.0% | |
| GAACAACATTAGAACATCAA+AGG | - | chr7.4:31901390-31901409 | MS.gene87069:CDS | 30.0% | |
| TGAAGATTCTATTGCTTGTT+TGG | + | chr7.4:31900718-31900737 | None:intergenic | 30.0% | |
| ! | AAGATTTTCTTGGATTGCTT+TGG | + | chr7.4:31900673-31900692 | None:intergenic | 30.0% |
| ! | ATCAAGTAGTTGAACTTTTC+TGG | + | chr7.4:31901022-31901041 | None:intergenic | 30.0% |
| ! | TCAAAGTGTTCAAGCAAATA+TGG | - | chr7.4:31901476-31901495 | MS.gene87069:CDS | 30.0% |
| ! | TTTTACAAGCTTAGAGACAT+GGG | - | chr7.4:31900551-31900570 | MS.gene87069:CDS | 30.0% |
| !! | ATTTTCCGGTTTGAAATTCT+TGG | + | chr7.4:31901226-31901245 | None:intergenic | 30.0% |
| !! | TTTGATGATGATATTCTTGG+TGG | + | chr7.4:31900749-31900768 | None:intergenic | 30.0% |
| !!! | CTTTGTTTTTGATGTGTTTG+AGG | + | chr7.4:31900642-31900661 | None:intergenic | 30.0% |
| AAACACCAACAACACAACAA+AGG | - | chr7.4:31900588-31900607 | MS.gene87069:CDS | 35.0% | |
| AACCGGAAAATTCAAACACA+TGG | - | chr7.4:31901235-31901254 | MS.gene87069:CDS | 35.0% | |
| AAGTCATCATTGAATCCACA+AGG | - | chr7.4:31901331-31901350 | MS.gene87069:CDS | 35.0% | |
| AGATTTCAGAGAATCAATGG+TGG | - | chr7.4:31901354-31901373 | MS.gene87069:CDS | 35.0% | |
| AGCTTGTAAAACCAAGCATT+AGG | + | chr7.4:31900542-31900561 | None:intergenic | 35.0% | |
| AGGAGATTTCAGAGAATCAA+TGG | - | chr7.4:31901351-31901370 | MS.gene87069:CDS | 35.0% | |
| CTAACAGAGAATCTTCTACT+TGG | + | chr7.4:31901172-31901191 | None:intergenic | 35.0% | |
| CTATCAAGTGGAGAATCAAA+TGG | + | chr7.4:31900818-31900837 | None:intergenic | 35.0% | |
| TTTCTTGGATTGCTTTGGTA+GGG | + | chr7.4:31900668-31900687 | None:intergenic | 35.0% | |
| ! | GTTTTACAAGCTTAGAGACA+TGG | - | chr7.4:31900550-31900569 | MS.gene87069:CDS | 35.0% |
| ! | TTCTTCCTTTGTTGTGTTGT+TGG | + | chr7.4:31900596-31900615 | None:intergenic | 35.0% |
| ! | TTTGTTGTGTTGTTGGTGTT+TGG | + | chr7.4:31900589-31900608 | None:intergenic | 35.0% |
| ! | TTTTCTTGGATTGCTTTGGT+AGG | + | chr7.4:31900669-31900688 | None:intergenic | 35.0% |
| !! | AGATTTTGAAGGAAGAACAG+AGG | - | chr7.4:31901128-31901147 | MS.gene87069:CDS | 35.0% |
| !!! | GTAGTTGAACTTTTCTGGTT+TGG | + | chr7.4:31901017-31901036 | None:intergenic | 35.0% |
| !!! | GTCTTTGGTGTTCTTCTTTT+TGG | + | chr7.4:31900782-31900801 | None:intergenic | 35.0% |
| !!! | TTTTCTGGTTTGGTCACAAT+TGG | + | chr7.4:31901007-31901026 | None:intergenic | 35.0% |
| AACTATCCGAAAGACTTCTC+CGG | + | chr7.4:31901300-31901319 | None:intergenic | 40.0% | |
| AGATTCTCTGTTAGTTCACC+AGG | - | chr7.4:31901178-31901197 | MS.gene87069:CDS | 40.0% | |
| GGAAAATTCAAACACATGGC+CGG | - | chr7.4:31901239-31901258 | MS.gene87069:CDS | 40.0% | |
| TCGATTATGATGTCGTTCGT+CGG | + | chr7.4:31900899-31900918 | None:intergenic | 40.0% | |
| TTGATCCTAAGTTTCACACC+TGG | + | chr7.4:31901199-31901218 | None:intergenic | 40.0% | |
| ! | GGAGATGATGATGAAGTCTT+TGG | + | chr7.4:31900797-31900816 | None:intergenic | 40.0% |
| ! | GGCCATGTGTTTGAATTTTC+CGG | + | chr7.4:31901240-31901259 | None:intergenic | 40.0% |
| !! | GATTCTCTGAAATCTCCTTG+TGG | + | chr7.4:31901349-31901368 | None:intergenic | 40.0% |
| ACACGGTCTGTTCTGATTTC+TGG | + | chr7.4:31900869-31900888 | None:intergenic | 45.0% | |
| CAGACATGATGCCTAATGCT+TGG | - | chr7.4:31900528-31900547 | MS.gene87069:CDS | 45.0% | |
| GATTCTGCAGAGCTATCAAG+TGG | + | chr7.4:31900830-31900849 | None:intergenic | 45.0% | |
| GTTCACCAGGTGTGAAACTT+AGG | - | chr7.4:31901191-31901210 | MS.gene87069:CDS | 45.0% | |
| TACGTCTTCCACCATCGTTT+CGG | - | chr7.4:31901267-31901286 | MS.gene87069:CDS | 45.0% | |
| ! | TGGTGGAAGACGTACTTTTC+CGG | + | chr7.4:31901261-31901280 | None:intergenic | 45.0% |
| !! | CTGTTCTGATTTCTGGCTCA+GGG | + | chr7.4:31900862-31900881 | None:intergenic | 45.0% |
| !! | TCTGGTTTGGTCACAATTGG+TGG | + | chr7.4:31901004-31901023 | None:intergenic | 45.0% |
| !! | TCTGTTCTGATTTCTGGCTC+AGG | + | chr7.4:31900863-31900882 | None:intergenic | 45.0% |
| CGTTCGTCGGAAGAAGAACA+CGG | + | chr7.4:31900886-31900905 | None:intergenic | 50.0% | |
| TGGCAACCGGAGAAGTCTTT+CGG | - | chr7.4:31901291-31901310 | MS.gene87069:CDS | 50.0% | |
| TCTTCCACCATCGTTTCGGC+TGG | - | chr7.4:31901271-31901290 | MS.gene87069:CDS | 55.0% | |
| CCATCGTTTCGGCTGGCAAC+CGG | - | chr7.4:31901278-31901297 | MS.gene87069:CDS | 60.0% | |
| GTTGCCAGCCGAAACGATGG+TGG | + | chr7.4:31901278-31901297 | None:intergenic | 60.0% | |
| CCGGTTGCCAGCCGAAACGA+TGG | + | chr7.4:31901281-31901300 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 31900503 | 31901522 | 31900503 | ID=MS.gene87069 |
| chr7.4 | mRNA | 31900503 | 31901522 | 31900503 | ID=MS.gene87069.t1;Parent=MS.gene87069 |
| chr7.4 | exon | 31900503 | 31901522 | 31900503 | ID=MS.gene87069.t1.exon1;Parent=MS.gene87069.t1 |
| chr7.4 | CDS | 31900503 | 31901522 | 31900503 | ID=cds.MS.gene87069.t1;Parent=MS.gene87069.t1 |
| Gene Sequence |
| Protein sequence |
