Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87122.t1 | XP_024627301.1 | 81.9 | 105 | 19 | 0 | 1 | 105 | 1 | 105 | 4.70E-37 | 163.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87122.t1 | Q6IP65 | 56.2 | 73 | 30 | 1 | 33 | 105 | 28 | 98 | 1.5e-12 | 73.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87122.t1 | A0A396GV60 | 81.9 | 105 | 19 | 0 | 1 | 105 | 1 | 105 | 3.4e-37 | 163.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene87122.t1 | TR | Rcd1-like |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87122.t1 | MTR_5g093460 | 85.542 | 83 | 9 | 1 | 24 | 106 | 1 | 80 | 6.75e-41 | 140 |
| MS.gene87122.t1 | MTR_5g093460 | 62.000 | 100 | 35 | 1 | 5 | 104 | 144 | 240 | 6.02e-33 | 119 |
| MS.gene87122.t1 | MTR_6g065170 | 67.619 | 105 | 32 | 1 | 1 | 105 | 11 | 113 | 5.36e-36 | 124 |
| MS.gene87122.t1 | MTR_3g018640 | 61.538 | 104 | 38 | 1 | 3 | 106 | 13 | 114 | 1.04e-33 | 118 |
| MS.gene87122.t1 | MTR_8g106940 | 63.208 | 106 | 37 | 1 | 1 | 106 | 11 | 114 | 1.21e-33 | 118 |
| MS.gene87122.t1 | MTR_1g056470 | 58.333 | 96 | 38 | 1 | 11 | 106 | 17 | 110 | 5.17e-28 | 102 |
| MS.gene87122.t1 | MTR_3g053170 | 60.759 | 79 | 29 | 1 | 28 | 106 | 26 | 102 | 3.25e-22 | 88.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87122.t1 | AT3G20800 | 53.333 | 105 | 41 | 3 | 7 | 106 | 14 | 115 | 2.48e-23 | 91.7 |
| MS.gene87122.t1 | AT5G12980 | 50.588 | 85 | 40 | 1 | 22 | 106 | 29 | 111 | 6.98e-21 | 85.1 |
Find 17 sgRNAs with CRISPR-Local
Find 93 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGAAACTTGCACCTTTATTA+TGG | 0.178926 | 7.4:+63165475 | MS.gene87122:CDS |
| CCTTTATTATGGAATTCTTA+TGG | 0.279542 | 7.4:+63165486 | MS.gene87122:CDS |
| CATAATAAAGGTGCAAGTTT+CGG | 0.333949 | 7.4:-63165474 | None:intergenic |
| TTTGTGCTGTGGTGCTAGAT+TGG | 0.371012 | 7.4:-63163853 | None:intergenic |
| CCATAAGAATTCCATAATAA+AGG | 0.371929 | 7.4:-63165486 | None:intergenic |
| AACTCGAGTGTGCAATGTTC+TGG | 0.374857 | 7.4:+63166276 | MS.gene87122:CDS |
| TTGTGCTGTGGTGCTAGATT+GGG | 0.395336 | 7.4:-63163852 | None:intergenic |
| GATTGGGATGAAGCAGTTAG+AGG | 0.438610 | 7.4:-63163836 | None:intergenic |
| TTCAATAACAAGGCGTTCCA+TGG | 0.458343 | 7.4:-63163885 | None:intergenic |
| AGATTCAACAGCGGAATAGT+AGG | 0.487502 | 7.4:-63166238 | None:intergenic |
| AGCACAAAGCATGGCATCCA+TGG | 0.505480 | 7.4:+63163868 | MS.gene87122:CDS |
| TGCTCTTCGTGTTCTCTCCA+AGG | 0.526049 | 7.4:+63163934 | MS.gene87122:CDS |
| GGATGCCATGCTTTGTGCTG+TGG | 0.548520 | 7.4:-63163864 | None:intergenic |
| TCCGCTGTTGAATCTTACTC+AGG | 0.564161 | 7.4:+63166246 | MS.gene87122:CDS |
| TAGCACCACAGCACAAAGCA+TGG | 0.612717 | 7.4:+63163859 | MS.gene87122:CDS |
| GATTGTTGAGTTCAATAACA+AGG | 0.670356 | 7.4:-63163895 | None:intergenic |
| GCCTGAGTAAGATTCAACAG+CGG | 0.773865 | 7.4:-63166247 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATGATTAACATATATTAAAA+TGG | - | chr7.4:63165263-63165282 | None:intergenic | 10.0% |
| !! | TAAAAAATAATATTGTGTAT+GGG | + | chr7.4:63165661-63165680 | MS.gene87122:intron | 10.0% |
| !! | TATGTTAATCATTATTAATT+AGG | + | chr7.4:63165271-63165290 | MS.gene87122:intron | 10.0% |
| !! | TGATTAACATATATTAAAAT+GGG | - | chr7.4:63165262-63165281 | None:intergenic | 10.0% |
| !! | TTAAAAAATAATATTGTGTA+TGG | + | chr7.4:63165660-63165679 | MS.gene87122:intron | 10.0% |
| !!! | AAAAAATTAACTTGAATAAT+TGG | + | chr7.4:63164606-63164625 | MS.gene87122:intron | 10.0% |
| !! | TTCAAAAGTTAAAAAAAACA+AGG | - | chr7.4:63165796-63165815 | None:intergenic | 15.0% |
| !! | TTGAAAACATAAACATAAAT+TGG | - | chr7.4:63165868-63165887 | None:intergenic | 15.0% |
| !!! | AATGTCTTTATATGATTTTT+TGG | + | chr7.4:63165082-63165101 | MS.gene87122:intron | 15.0% |
| !!! | AGAAAATGAAAATTTTCATT+TGG | + | chr7.4:63165189-63165208 | MS.gene87122:intron | 15.0% |
| !!! | TACTATTGAAATATTTTTAG+AGG | + | chr7.4:63165509-63165528 | MS.gene87122:CDS | 15.0% |
| !!! | TTTTATTTTTGGGTTTTTTT+GGG | + | chr7.4:63164155-63164174 | MS.gene87122:intron | 15.0% |
| !!! | TTTTTATTTTTGGGTTTTTT+TGG | + | chr7.4:63164154-63164173 | MS.gene87122:intron | 15.0% |
| !! | GAAAAAAGTTATTATGCATT+TGG | + | chr7.4:63165702-63165721 | MS.gene87122:intron | 20.0% |
| !! | GTTTAATATTGTAACATCAA+TGG | + | chr7.4:63166045-63166064 | MS.gene87122:intron | 20.0% |
| !! | TTAATAGGATGAAATGAAAT+AGG | + | chr7.4:63164537-63164556 | MS.gene87122:intron | 20.0% |
| !!! | CCATTTTTAATATCTTTCAT+GGG | + | chr7.4:63163983-63164002 | MS.gene87122:intron | 20.0% |
| !!! | GAAGATTTGTTTCAAATTTA+AGG | + | chr7.4:63164292-63164311 | MS.gene87122:intron | 20.0% |
| !!! | GGTGAGAATAATATTTTATT+AGG | + | chr7.4:63165292-63165311 | MS.gene87122:intron | 20.0% |
| !!! | TAATATTTTATTAGGTGCTA+TGG | + | chr7.4:63165300-63165319 | MS.gene87122:intron | 20.0% |
| !!! | TCCATTTTTAATATCTTTCA+TGG | + | chr7.4:63163982-63164001 | MS.gene87122:intron | 20.0% |
| !!! | TTGGAATAACTATGATTTTT+TGG | + | chr7.4:63165101-63165120 | MS.gene87122:intron | 20.0% |
| !!! | TTTATTTTTGGGTTTTTTTG+GGG | + | chr7.4:63164156-63164175 | MS.gene87122:intron | 20.0% |
| !!! | TTTCATTTTTTAAGTTTCAG+AGG | + | chr7.4:63165439-63165458 | MS.gene87122:intron | 20.0% |
| !!! | TTTTTTTAAAATTGTCCAAG+AGG | + | chr7.4:63166003-63166022 | MS.gene87122:intron | 20.0% |
| ! | ACAAAAGAGACATGATTATA+AGG | + | chr7.4:63165936-63165955 | MS.gene87122:intron | 25.0% |
| ! | CAAAAGAGACATGATTATAA+GGG | + | chr7.4:63165937-63165956 | MS.gene87122:intron | 25.0% |
| ! | CCATAAGAATTCCATAATAA+AGG | - | chr7.4:63165489-63165508 | None:intergenic | 25.0% |
| ! | CCCATGAAAGATATTAAAAA+TGG | - | chr7.4:63163986-63164005 | None:intergenic | 25.0% |
| ! | CCTTTATTATGGAATTCTTA+TGG | + | chr7.4:63165486-63165505 | MS.gene87122:CDS | 25.0% |
| ! | GTAAGAATGTGATAGTTAAT+AGG | + | chr7.4:63164522-63164541 | MS.gene87122:intron | 25.0% |
| ! | TAAGAAAGAGATGATTAGAA+AGG | + | chr7.4:63164482-63164501 | MS.gene87122:intron | 25.0% |
| ! | TGAATCTATGAAGCATAAAA+AGG | + | chr7.4:63164457-63164476 | MS.gene87122:intron | 25.0% |
| ! | TGTTACAAAATTGCATTAGT+TGG | + | chr7.4:63164358-63164377 | MS.gene87122:intron | 25.0% |
| !! | AGATTGATCATTTGTTACTA+AGG | + | chr7.4:63165630-63165649 | MS.gene87122:intron | 25.0% |
| !! | GATATATTGAAGTTATTTCC+TGG | - | chr7.4:63166219-63166238 | None:intergenic | 25.0% |
| !! | GATCTAGATTTTATGACAAA+TGG | - | chr7.4:63164783-63164802 | None:intergenic | 25.0% |
| !! | GTTCAAATATTTGTTGAGAA+AGG | - | chr7.4:63164997-63165016 | None:intergenic | 25.0% |
| !! | TGTGTAATTCTTTATTTCGA+GGG | + | chr7.4:63164413-63164432 | MS.gene87122:intron | 25.0% |
| !! | TTGTGTAATTCTTTATTTCG+AGG | + | chr7.4:63164412-63164431 | MS.gene87122:intron | 25.0% |
| !!! | TCTAAGAGTATTACAAACAA+GGG | - | chr7.4:63165611-63165630 | None:intergenic | 25.0% |
| !!! | TTTTTAAGTTTCAGAGGATT+GGG | + | chr7.4:63165445-63165464 | MS.gene87122:intron | 25.0% |
| !!! | TTTTTTAAGTTTCAGAGGAT+TGG | + | chr7.4:63165444-63165463 | MS.gene87122:intron | 25.0% |
| ACATGATTATAAGGGAGAAT+TGG | + | chr7.4:63165945-63165964 | MS.gene87122:intron | 30.0% | |
| AGGGAGAATTGGAAAATAAA+AGG | + | chr7.4:63165956-63165975 | MS.gene87122:intron | 30.0% | |
| ATATGACTAGAATCTCATTC+TGG | - | chr7.4:63165131-63165150 | None:intergenic | 30.0% | |
| GATTGTTGAGTTCAATAACA+AGG | - | chr7.4:63163898-63163917 | None:intergenic | 30.0% | |
| TGGACAAAAGAAAAGTAGAT+TGG | - | chr7.4:63163966-63163985 | None:intergenic | 30.0% | |
| TTATGCTTCATAGATTCACA+GGG | - | chr7.4:63164455-63164474 | None:intergenic | 30.0% | |
| TTCTATCATGAAAGTCAGAT+TGG | - | chr7.4:63164653-63164672 | None:intergenic | 30.0% | |
| TTTATGCTTCATAGATTCAC+AGG | - | chr7.4:63164456-63164475 | None:intergenic | 30.0% | |
| ! | AAGTAGATTGGTAATAACCT+TGG | - | chr7.4:63163954-63163973 | None:intergenic | 30.0% |
| ! | CAGATAGTGATAATTTTTCC+AGG | + | chr7.4:63166198-63166217 | MS.gene87122:intron | 30.0% |
| ! | CATAATAAAGGTGCAAGTTT+CGG | - | chr7.4:63165477-63165496 | None:intergenic | 30.0% |
| ! | TGTATGGGTCTTAATTTTAG+TGG | + | chr7.4:63165676-63165695 | MS.gene87122:intron | 30.0% |
| !! | CTCTAAGAGTATTACAAACA+AGG | - | chr7.4:63165612-63165631 | None:intergenic | 30.0% |
| ATGTGACTAAGTCTACTGAA+AGG | - | chr7.4:63165048-63165067 | None:intergenic | 35.0% | |
| CGAAACTTGCACCTTTATTA+TGG | + | chr7.4:63165475-63165494 | MS.gene87122:CDS | 35.0% | |
| CGCTTTACATGTTCTCTTAA+AGG | + | chr7.4:63164861-63164880 | MS.gene87122:intron | 35.0% | |
| GTTCATTCTTTAGTGTATCG+TGG | + | chr7.4:63164217-63164236 | MS.gene87122:intron | 35.0% | |
| GTTCTTTGCTTTAGTACATC+TGG | + | chr7.4:63165575-63165594 | MS.gene87122:intron | 35.0% | |
| TAAGTTCAAATCTCCTCAAG+CGG | + | chr7.4:63166070-63166089 | MS.gene87122:intron | 35.0% | |
| TAGATAATTCCTCGAAGAGT+TGG | - | chr7.4:63164955-63164974 | None:intergenic | 35.0% | |
| TCTCAAGAAATCCAGATCTT+TGG | + | chr7.4:63164008-63164027 | MS.gene87122:intron | 35.0% | |
| TGTGACTAAGTCTACTGAAA+GGG | - | chr7.4:63165047-63165066 | None:intergenic | 35.0% | |
| TTTGTGATATGCAAGTGAGA+TGG | - | chr7.4:63164807-63164826 | None:intergenic | 35.0% | |
| ! | TTGCTTAGTTCTCGATAATG+TGG | + | chr7.4:63165388-63165407 | MS.gene87122:intron | 35.0% |
| !!! | AGAGCATTTTCTCTAAGATC+AGG | - | chr7.4:63163920-63163939 | None:intergenic | 35.0% |
| ACATGTAATCCAACTCTTCG+AGG | + | chr7.4:63164943-63164962 | MS.gene87122:intron | 40.0% | |
| AGATTCAACAGCGGAATAGT+AGG | - | chr7.4:63166241-63166260 | None:intergenic | 40.0% | |
| GCTTTAGTACATCTGGCAAA+CGG | + | chr7.4:63165582-63165601 | MS.gene87122:intron | 40.0% | |
| GGATTACATGTGTGTGTTCA+GGG | - | chr7.4:63164934-63164953 | None:intergenic | 40.0% | |
| GTGACTAAGTCTACTGAAAG+GGG | - | chr7.4:63165046-63165065 | None:intergenic | 40.0% | |
| TCACAACTTCTCCAAAGATC+TGG | - | chr7.4:63164022-63164041 | None:intergenic | 40.0% | |
| TGGATTACATGTGTGTGTTC+AGG | - | chr7.4:63164935-63164954 | None:intergenic | 40.0% | |
| ! | TTCAATAACAAGGCGTTCCA+TGG | - | chr7.4:63163888-63163907 | None:intergenic | 40.0% |
| !! | CTGCACTATCACACTAGTTT+TGG | + | chr7.4:63164109-63164128 | MS.gene87122:intron | 40.0% |
| !!! | TGTCATTTTACAACCGCTTG+AGG | - | chr7.4:63166086-63166105 | None:intergenic | 40.0% |
| AACTCGAGTGTGCAATGTTC+TGG | + | chr7.4:63166276-63166295 | MS.gene87122:CDS | 45.0% | |
| GATTGGGATGAAGCAGTTAG+AGG | - | chr7.4:63163839-63163858 | None:intergenic | 45.0% | |
| GCCTGAGTAAGATTCAACAG+CGG | - | chr7.4:63166250-63166269 | None:intergenic | 45.0% | |
| GGCGAAAAACACAACAGTAG+TGG | + | chr7.4:63165723-63165742 | MS.gene87122:intron | 45.0% | |
| TCCGCTGTTGAATCTTACTC+AGG | + | chr7.4:63166246-63166265 | MS.gene87122:CDS | 45.0% | |
| ! | TTGTGCTGTGGTGCTAGATT+GGG | - | chr7.4:63163855-63163874 | None:intergenic | 45.0% |
| ! | TTTGTGCTGTGGTGCTAGAT+TGG | - | chr7.4:63163856-63163875 | None:intergenic | 45.0% |
| !!! | ATTTTATTGTTTTTATTTTT+GGG | + | chr7.4:63164145-63164164 | MS.gene87122:intron | 5.0% |
| !!! | TATTTTATTGTTTTTATTTT+TGG | + | chr7.4:63164144-63164163 | MS.gene87122:intron | 5.0% |
| ACCACTGAGCTACATCCTCT+TGG | - | chr7.4:63166021-63166040 | None:intergenic | 50.0% | |
| AGCACAAAGCATGGCATCCA+TGG | + | chr7.4:63163868-63163887 | MS.gene87122:CDS | 50.0% | |
| TAGCACCACAGCACAAAGCA+TGG | + | chr7.4:63163859-63163878 | MS.gene87122:CDS | 50.0% | |
| TCCAAGAGGATGTAGCTCAG+TGG | + | chr7.4:63166017-63166036 | MS.gene87122:intron | 50.0% | |
| TGCTCTTCGTGTTCTCTCCA+AGG | + | chr7.4:63163934-63163953 | MS.gene87122:CDS | 50.0% | |
| GGATGCCATGCTTTGTGCTG+TGG | - | chr7.4:63163867-63163886 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 63163809 | 63166318 | 63163809 | ID=MS.gene87122 |
| chr7.4 | mRNA | 63163809 | 63166318 | 63163809 | ID=MS.gene87122.t1;Parent=MS.gene87122 |
| chr7.4 | exon | 63163809 | 63163955 | 63163809 | ID=MS.gene87122.t1.exon1;Parent=MS.gene87122.t1 |
| chr7.4 | CDS | 63163809 | 63163955 | 63163809 | ID=cds.MS.gene87122.t1;Parent=MS.gene87122.t1 |
| chr7.4 | exon | 63165453 | 63165530 | 63165453 | ID=MS.gene87122.t1.exon2;Parent=MS.gene87122.t1 |
| chr7.4 | CDS | 63165453 | 63165530 | 63165453 | ID=cds.MS.gene87122.t1;Parent=MS.gene87122.t1 |
| chr7.4 | exon | 63166220 | 63166318 | 63166220 | ID=MS.gene87122.t1.exon3;Parent=MS.gene87122.t1 |
| chr7.4 | CDS | 63166220 | 63166318 | 63166220 | ID=cds.MS.gene87122.t1;Parent=MS.gene87122.t1 |
| Gene Sequence |
| Protein sequence |
