AlfalfaGEDB | MS.gene87122

M. sativa cultivar XinJiangDaYe / MS.gene87122

KEGG: K12606
GO: -
PFAM: PF04078.16

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene87122.t1	XP_024627301.1	81.9	105	19	0	1	105	1	105	4.70E-37	163.7

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene87122.t1	Q6IP65	56.2	73	30	1	33	105	28	98	1.5e-12	73.6

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene87122.t1	A0A396GV60	81.9	105	19	0	1	105	1	105	3.4e-37	163.7

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene87122.t1	TR	Rcd1-like

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene87122.t1	MTR_5g093460	85.542	83	9	1	24	106	1	80	6.75e-41	140
MS.gene87122.t1	MTR_5g093460	62.000	100	35	1	5	104	144	240	6.02e-33	119
MS.gene87122.t1	MTR_6g065170	67.619	105	32	1	1	105	11	113	5.36e-36	124
MS.gene87122.t1	MTR_3g018640	61.538	104	38	1	3	106	13	114	1.04e-33	118
MS.gene87122.t1	MTR_8g106940	63.208	106	37	1	1	106	11	114	1.21e-33	118
MS.gene87122.t1	MTR_1g056470	58.333	96	38	1	11	106	17	110	5.17e-28	102
MS.gene87122.t1	MTR_3g053170	60.759	79	29	1	28	106	26	102	3.25e-22	88.6

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene87122.t1	AT3G20800	53.333	105	41	3	7	106	14	115	2.48e-23	91.7
MS.gene87122.t1	AT5G12980	50.588	85	40	1	22	106	29	111	6.98e-21	85.1

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Find 17 sgRNAs with CRISPR-Local

Find 93 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACTCGAGTGTGCAATGTTC+TGG	0.374857	7.4:+63166276	MS.gene87122:CDS
AGATTCAACAGCGGAATAGT+AGG	0.487502	7.4:-63166238	None:intergenic
AGCACAAAGCATGGCATCCA+TGG	0.505480	7.4:+63163868	MS.gene87122:CDS
CATAATAAAGGTGCAAGTTT+CGG	0.333949	7.4:-63165474	None:intergenic
CCATAAGAATTCCATAATAA+AGG	0.371929	7.4:-63165486	None:intergenic
CCTTTATTATGGAATTCTTA+TGG	0.279542	7.4:+63165486	MS.gene87122:CDS
CGAAACTTGCACCTTTATTA+TGG	0.178926	7.4:+63165475	MS.gene87122:CDS
GATTGGGATGAAGCAGTTAG+AGG	0.438610	7.4:-63163836	None:intergenic
GATTGTTGAGTTCAATAACA+AGG	0.670356	7.4:-63163895	None:intergenic
GCCTGAGTAAGATTCAACAG+CGG	0.773865	7.4:-63166247	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ACATGATTATAAGGGAGAAT+TGG	+	chr7.4:63165945-63165964	MS.gene87122:intron	30.0%
AGGGAGAATTGGAAAATAAA+AGG	+	chr7.4:63165956-63165975	MS.gene87122:intron	30.0%
ATATGACTAGAATCTCATTC+TGG	-	chr7.4:63165131-63165150	None:intergenic	30.0%
GATTGTTGAGTTCAATAACA+AGG	-	chr7.4:63163898-63163917	None:intergenic	30.0%
TGGACAAAAGAAAAGTAGAT+TGG	-	chr7.4:63163966-63163985	None:intergenic	30.0%
TTATGCTTCATAGATTCACA+GGG	-	chr7.4:63164455-63164474	None:intergenic	30.0%
TTCTATCATGAAAGTCAGAT+TGG	-	chr7.4:63164653-63164672	None:intergenic	30.0%
TTTATGCTTCATAGATTCAC+AGG	-	chr7.4:63164456-63164475	None:intergenic	30.0%
ATGTGACTAAGTCTACTGAA+AGG	-	chr7.4:63165048-63165067	None:intergenic	35.0%
CGAAACTTGCACCTTTATTA+TGG	+	chr7.4:63165475-63165494	MS.gene87122:CDS	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr7.4	gene	63163809	63166318	63163809	ID=MS.gene87122
chr7.4	mRNA	63163809	63166318	63163809	ID=MS.gene87122.t1;Parent=MS.gene87122
chr7.4	exon	63163809	63163955	63163809	ID=MS.gene87122.t1.exon1;Parent=MS.gene87122.t1
chr7.4	CDS	63163809	63163955	63163809	ID=cds.MS.gene87122.t1;Parent=MS.gene87122.t1
chr7.4	exon	63165453	63165530	63165453	ID=MS.gene87122.t1.exon2;Parent=MS.gene87122.t1
chr7.4	CDS	63165453	63165530	63165453	ID=cds.MS.gene87122.t1;Parent=MS.gene87122.t1
chr7.4	exon	63166220	63166318	63166220	ID=MS.gene87122.t1.exon3;Parent=MS.gene87122.t1
chr7.4	CDS	63166220	63166318	63166220	ID=cds.MS.gene87122.t1;Parent=MS.gene87122.t1

Gene Sequence

>MS.gene87122

ATGAATAACGTTGCGTCGTTTGTAAGTCCTCTAACTGCTTCATCCCAATCTAGCACCACAGCACAAAGCATGGCATCCATGGAACGCCTTGTTATTGAACTCAACAATCCTGATCTTAGAGAAAATGCTCTTCGTGTTCTCTCCAAGGTTATTACCAATCTACTTTTCTTTTGTCCATTTTTAATATCTTTCATGGGATTCTCAAGAAATCCAGATCTTTGGAGAAGTTGTGATTCTCTGAAACTGTTATTTAAATTGTTTGACTGCTTCTTTGTCAACATTGATTCAAATTTTGATACACTGCACTATCACACTAGTTTTGGTAGACTTATTAATATTTTATTGTTTTTATTTTTGGGTTTTTTTGGGGCATAAAGATGAGATTCAAGTCATAATTGAGTTAAAAGAGTTCATTCTTTAGTGTATCGTGGCTGAAAATTTTGATTCTTTTTAAACTAAATATAATATTTTTTCTATATATTTGAAGATTTGTTTCAAATTTAAGGTATGTTAATCATTGTGAATTAGATGAATTAATCTCTTTTAATTTGTTACAAAATTGCATTAGTTGGTGTCATAATTGTCAAAAAATTGATATTGTTATTGTGTAATTCTTTATTTCGAGGGCAATGTTTATGTTAGACCCTGTGAATCTATGAAGCATAAAAAGGTGTAAGAAAGAGATGATTAGAAAGGAAATATAGAGAGTGAAAGTAAGAATGTGATAGTTAATAGGATGAAATGAAATAGGATAAAAGAAACAGTAGACATAAAATGTTATATGATATATAAGAAGAAAAAAATTAACTTGAATAATTGGACTCGAATTCACACAACTTAGCCAATCTGACTTTCATGATAGAAATCAAGTGACTTATGTTGTAACTTCAACAAATAAAGAACTTTATAGTGTGACTTTTTTTAACAGTCGAAGTCATTGATTGCAATGATCAATGTTGACAACATCAATTCCATTTGTCATAAAATCTAGATCACCATCTCACTTGCATATCACAAAATTTTAGAATCAAGAATCTTGATCTTCGATAAAACGCTTTACATGTTCTCTTAAAGGTTTCTGCAATTATTCTTTAAATTTTTTATTCTATTTTTCATGTCTTTCCCTGAACACACACATGTAATCCAACTCTTCGAGGAATTATCTAGCATTAAACTAAATGTTATCCTTTCTCAACAAATATTTGAACTATGATACGCTTCATTGTGAGACTATCCCCTTTCAGTAGACTTAGTCACATTTGATTGTGATTTTAATGTCTTTATATGATTTTTTGGAATAACTATGATTTTTTGGAGAACCAGAATGAGATTCTAGTCATATACTTGAATGAAAAATCTTCATCATTTAGAATAATAGTGAGAAAATGAAAATTTTCATTTGGTTTTTGTATAAAAAATATGATATATTTATATAACTTGAAGTATTCTTCCCATTTTAATATATGTTAATCATTATTAATTAGGTGAGAATAATATTTTATTAGGTGCTATGGACTTGCACTACTTGATATCTAAAATTTAGAACTTTTATTCTATTTTAGAGCTTTTATATTACAGTTTGCTTAGTTCTCGATAATGTGGCTCGAACTCATGCAATTAATACTTTATTTTTCATTTTTTAAGTTTCAGAGGATTGGGTTATATCCGAAACTTGCACCTTTATTATGGAATTCTTATGGTACTATTGAAATATTTTTAGAGGTATGTAATATGTGACTGACATTATAATTCATCAACTAGTTGTTGTTCTTTGCTTTAGTACATCTGGCAAACGGTTTCCCTTGTTTGTAATACTCTTAGAGATTGATCATTTGTTACTAAGGAAGATTTTTAAAAAATAATATTGTGTATGGGTCTTAATTTTAGTGGATAGAAAAAAGTTATTATGCATTTGGCGAAAAACACAACAGTAGTGGTTGAAAATAATATACGTTGTTCTTTAACGTATTTACGAACATAATTTCCTTGTTTTTTTTAACTTTTGAATCTGTTTATCTCTGACTATTTGCTTGTTTTCTACAGTTTCATTCTTTCACCAATTTATGTTTATGTTTTCAACTTTTACTACTGAACGTGAAAACAATATTATTTATTTTAACAATTCAAACAAAAGAGACATGATTATAAGGGAGAATTGGAAAATAAAAGGTGATGTTATAAATCAATTTAGAAGTTTTTTTAAAATTGTCCAAGAGGATGTAGCTCAGTGGTAATAGTTTAATATTGTAACATCAATGGAATAAGTTCAAATCTCCTCAAGCGGTTGTAAAATGACAATTGATGTGACTCTTATCAATACTGTTTCTTCTTGATTGCTTAAGTTTAATAGTACTTTACTAAATATGTTTATTGATTTCAATACACTTATCAGATAGTGATAATTTTTCCAGGAAATAACTTCAATATATCCTACTATTCCGCTGTTGAATCTTACTCAGGCACAATCAACTCGAGTGTGCAATGTTCTGGAACTACTTCAAGTGCGATGA

Protein sequence

>MS.gene87122.t1

MNNVASFVSPLTASSQSSTTAQSMASMERLVIELNNPDLRENALRVLSKFQRIGLYPKLAPLLWNSYGTIEIFLEEITSIYPTIPLLNLTQAQSTRVCNVLELLQVR