AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene87162


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene87162.t1 MTR_7g035300 89.401 217 15 1 1 209 17 233 1.44e-144 402
MS.gene87162.t1 MTR_7g037260 43.096 239 80 6 26 208 119 357 8.54e-53 173
MS.gene87162.t1 MTR_7g037130 43.644 236 77 6 26 205 119 354 2.91e-49 164
MS.gene87162.t1 MTR_7g451170 40.698 258 89 7 15 208 154 411 6.97e-48 162
MS.gene87162.t1 MTR_4g088015 35.808 229 83 6 41 205 210 438 9.25e-36 130
MS.gene87162.t1 MTR_1g057980 33.068 251 95 6 16 193 44 294 2.09e-31 116
MS.gene87162.t1 MTR_7g035350 31.200 250 95 6 20 192 193 442 5.04e-26 104
MS.gene87162.t1 MTR_7g450950 39.286 196 77 5 21 174 125 320 3.37e-25 100
MS.gene87162.t1 MTR_3g077650 44.860 107 47 3 54 148 183 289 1.07e-20 89.4
MS.gene87162.t1 MTR_5g049190 42.202 109 51 3 48 144 15 123 6.96e-20 84.7
MS.gene87162.t1 MTR_4g105660 39.252 107 53 3 54 148 182 288 8.13e-19 84.0
MS.gene87162.t1 MTR_3g463400 35.652 115 62 3 54 156 135 249 8.16e-19 84.0
MS.gene87162.t1 MTR_5g070020 31.707 164 90 5 12 158 77 235 1.55e-18 82.8
MS.gene87162.t1 MTR_6g027340 38.614 101 52 3 75 169 50 146 2.12e-14 69.3
MS.gene87162.t1 MTR_7g061330 41.935 93 42 3 55 135 116 208 5.40e-14 70.5
MS.gene87162.t1 MTR_7g461410 41.935 93 42 3 55 135 116 208 5.40e-14 70.5
MS.gene87162.t1 MTR_7g061330 41.935 93 42 3 55 135 80 172 6.39e-14 70.1
MS.gene87162.t1 MTR_6g027360 38.384 99 51 3 77 169 114 208 7.45e-14 69.3
MS.gene87162.t1 MTR_5g010650 39.785 93 44 3 54 134 112 204 7.76e-14 69.7
MS.gene87162.t1 MTR_4g019370 37.391 115 52 5 55 149 41 155 1.23e-13 68.9
MS.gene87162.t1 MTR_1g026870 32.174 115 65 4 55 156 88 202 2.69e-13 68.6
MS.gene87162.t1 MTR_3g110028 33.913 115 63 4 55 156 88 202 5.93e-13 67.4
MS.gene87162.t1 MTR_4g128190 36.957 92 55 3 49 138 10 100 1.02e-11 62.4
MS.gene87162.t1 MTR_1g008970 39.796 98 55 4 77 171 127 223 1.24e-11 62.4
MS.gene87162.t1 MTR_4g082040 34.483 116 63 4 34 136 86 201 2.49e-11 62.4
MS.gene87162.t1 MTR_5g041570 35.455 110 54 4 55 147 137 246 2.67e-11 62.4
MS.gene87162.t1 MTR_3g052450 33.333 102 53 3 48 135 38 138 5.57e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene87162.t1 AT4G18770 40.000 115 57 3 46 148 211 325 9.63e-21 89.4
MS.gene87162.t1 AT3G27785 40.541 111 54 3 46 144 183 293 1.08e-19 86.7
MS.gene87162.t1 AT5G40360 40.678 118 56 3 44 148 150 266 1.38e-18 82.8
MS.gene87162.t1 AT2G25230 40.157 127 63 4 46 159 20 146 1.01e-17 79.0
MS.gene87162.t1 AT5G11050 39.640 111 55 3 47 145 100 210 1.09e-17 80.9
MS.gene87162.t1 AT5G58850 38.532 109 55 3 49 145 102 210 1.12e-17 80.9
MS.gene87162.t1 AT5G40430 35.758 165 89 6 46 193 48 212 2.31e-17 78.2
MS.gene87162.t1 AT5G11510 37.778 90 44 2 55 132 84 173 4.38e-13 67.8
MS.gene87162.t1 AT5G11510 37.778 90 44 2 55 132 84 173 4.38e-13 67.8
MS.gene87162.t1 AT5G11510 37.778 90 44 2 55 132 84 173 4.38e-13 67.8
MS.gene87162.t1 AT5G11510 37.778 90 44 2 55 132 84 173 4.84e-13 67.8
MS.gene87162.t1 AT5G11510 37.778 90 44 2 55 132 84 173 5.23e-13 67.8
MS.gene87162.t1 AT3G53200 41.667 72 40 2 62 132 1 71 8.21e-13 65.1
MS.gene87162.t1 AT5G02320 36.559 93 47 3 55 135 70 162 1.16e-12 66.6
MS.gene87162.t1 AT5G02320 36.559 93 47 3 55 135 130 222 1.21e-12 66.6
MS.gene87162.t1 AT5G02320 36.559 93 47 3 55 135 130 222 1.21e-12 66.6
MS.gene87162.t1 AT3G09370 38.710 93 45 3 55 135 133 225 2.18e-12 65.9
MS.gene87162.t1 AT3G09370 38.710 93 45 3 55 135 138 230 2.21e-12 65.5
MS.gene87162.t1 AT3G09370 38.710 93 45 3 55 135 146 238 2.51e-12 65.5
MS.gene87162.t1 AT3G28470 32.673 101 55 3 48 135 10 110 5.63e-12 63.9
MS.gene87162.t1 AT1G57560 29.545 132 82 4 77 202 51 177 7.54e-12 63.2
MS.gene87162.t1 AT1G69560 26.590 173 102 6 1 153 74 241 8.72e-12 63.5
MS.gene87162.t1 AT1G69560 29.134 127 73 4 44 153 99 225 9.13e-12 63.5
MS.gene87162.t1 AT4G33450 43.243 74 37 2 77 149 55 124 1.09e-11 62.8
MS.gene87162.t1 AT2G47190 31.193 109 62 3 37 132 7 115 1.15e-11 62.8
MS.gene87162.t1 AT3G53200 45.614 57 30 1 77 132 48 104 1.41e-11 62.0
MS.gene87162.t1 AT1G57560 40.580 69 40 1 77 144 51 119 2.39e-11 62.0
MS.gene87162.t1 AT4G32730 34.409 93 49 3 55 135 90 182 2.60e-11 62.8
MS.gene87162.t1 AT4G32730 34.409 93 49 3 55 135 90 182 2.85e-11 62.8
MS.gene87162.t1 AT4G32730 34.409 93 49 3 55 135 90 182 2.85e-11 62.8
MS.gene87162.t1 AT4G32730 34.409 93 49 3 55 135 90 182 2.85e-11 62.8
MS.gene87162.t1 AT4G32730 34.409 93 49 3 55 135 90 182 2.85e-11 62.8

Find 41 sgRNAs with CRISPR-Local

Find 66 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCCTTATGTGGAAACGTTTA+AGG 0.181442 7.4:+64017073 MS.gene87162:CDS
AACGATGATTATGAGTTATA+TGG 0.223163 7.4:+64016998 MS.gene87162:CDS
GGCATTCTCGACTTGCTTAA+TGG 0.229142 7.4:-64016867 None:intergenic
ACTACTCCAGAAGATGAATT+TGG 0.261057 7.4:+64016953 MS.gene87162:CDS
GATCAAGCCCACAACATATT+TGG 0.264284 7.4:+64016773 MS.gene87162:CDS
AAAAGAAACACCTTAATATC+TGG 0.291683 7.4:-64016260 None:intergenic
CCATTGATCAGCATTTATAT+TGG 0.314785 7.4:-64015999 None:intergenic
CCATCTTAAACCAGATATTA+AGG 0.331529 7.4:+64016250 MS.gene87162:CDS
GAATAGTATTCTGCTGCTTA+TGG 0.333313 7.4:-64015946 None:intergenic
GAAATTGATTTCCTGCTTCA+TGG 0.333503 7.4:-64017044 None:intergenic
ACCTTAAACGTTTCCACATA+AGG 0.341962 7.4:-64017074 None:intergenic
TATCCACCAAATTCATCTTC+TGG 0.354153 7.4:-64016959 None:intergenic
TAAGCAGCAGAATACTATTC+AGG 0.381837 7.4:+64015949 MS.gene87162:CDS
TAATCATCGTTGAATGTCAT+TGG 0.431930 7.4:-64016986 None:intergenic
TTCCTGCTTCATGGGAACCT+CGG 0.444432 7.4:-64017035 None:intergenic
TCCTGCTTCATGGGAACCTC+GGG 0.444652 7.4:-64017034 None:intergenic
ACAACATATTTGGAGACAAC+TGG 0.446422 7.4:+64016783 MS.gene87162:CDS
GTTGTCTCCAAATATGTTGT+GGG 0.463375 7.4:-64016780 None:intergenic
TGCTTATGGTAAATGAGAAC+GGG 0.465009 7.4:-64015932 None:intergenic
AATTTCATGAAGGCCTTATG+TGG 0.471697 7.4:+64017061 MS.gene87162:CDS
AAATTGATTTCCTGCTTCAT+GGG 0.472609 7.4:-64017043 None:intergenic
ATATTTGTATCCAATTTCAC+TGG 0.483235 7.4:-64015891 None:intergenic
CCTTAATATCTGGTTTAAGA+TGG 0.484241 7.4:-64016250 None:intergenic
AATGGACTACAAAAGAGAAT+AGG 0.491834 7.4:+64016017 MS.gene87162:CDS
TCCCGAGGTTCCCATGAAGC+AGG 0.502564 7.4:+64017033 MS.gene87162:CDS
AGTTGTCTCCAAATATGTTG+TGG 0.504440 7.4:-64016781 None:intergenic
GAAATTTCAAGAAGATTGCC+AGG 0.513813 7.4:+64016809 MS.gene87162:CDS
CCAATATAAATGCTGATCAA+TGG 0.515462 7.4:+64015999 MS.gene87162:CDS
TGAGAACGGGTTCTTGATCA+TGG 0.521600 7.4:-64015919 None:intergenic
CTGCTTATGGTAAATGAGAA+CGG 0.536086 7.4:-64015933 None:intergenic
TGGATCTCTTTCTAAATCGA+TGG 0.536456 7.4:+64016198 MS.gene87162:CDS
TAAAACAATGCATGGAGAGA+TGG 0.560064 7.4:+64016225 MS.gene87162:CDS
ATGGACCTTAAAACAATGCA+TGG 0.570560 7.4:+64016217 MS.gene87162:CDS
ACTCCAGAAGATGAATTTGG+TGG 0.588319 7.4:+64016956 MS.gene87162:CDS
TAAGGAACATCATATGGATG+AGG 0.593905 7.4:+64015844 None:intergenic
GCAGGAAATCAATTTCATGA+AGG 0.597528 7.4:+64017051 MS.gene87162:CDS
GAATGAGCCATTGAGTGAGG+AGG 0.611909 7.4:+64016736 MS.gene87162:intron
ACATAACTATCGTTACGTCC+TGG 0.612302 7.4:-64016827 None:intergenic
TGGTTGCAAAACTAGAGTGA+TGG 0.623138 7.4:-64015871 None:intergenic
TCGATTTAGAAAGAGATCCA+CGG 0.651610 7.4:-64016195 None:intergenic
GTAGAATGAGCCATTGAGTG+AGG 0.657428 7.4:+64016733 MS.gene87162:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTCATATATAGTTTTATTA+TGG + chr7.4:64016082-64016101 MS.gene87162:intron 10.0%
!! GTCTATATATAATTAAATAG+AGG - chr7.4:64016687-64016706 None:intergenic 15.0%
!! AAACCTTCAAAAATGTAATT+AGG - chr7.4:64016932-64016951 None:intergenic 20.0%
!! ATAAAAATATTGCACATGAA+TGG + chr7.4:64016635-64016654 MS.gene87162:intron 20.0%
!! TGCTAAAATAAGTGTTAAAA+AGG - chr7.4:64016291-64016310 None:intergenic 20.0%
!! TTACGCAATAAAAGATAAAA+AGG - chr7.4:64016318-64016337 None:intergenic 20.0%
!!! TATGATTTTATAACAACAAG+TGG + chr7.4:64016447-64016466 MS.gene87162:intron 20.0%
! AAAAAATACACACATCCATT+AGG - chr7.4:64016572-64016591 None:intergenic 25.0%
! AAAAGAAACACCTTAATATC+TGG - chr7.4:64016263-64016282 None:intergenic 25.0%
! AACGATGATTATGAGTTATA+TGG + chr7.4:64016998-64017017 MS.gene87162:CDS 25.0%
! ATATTTGTATCCAATTTCAC+TGG - chr7.4:64015894-64015913 None:intergenic 25.0%
! CATTTCATTGAAAAACCTAA+TGG + chr7.4:64016554-64016573 MS.gene87162:intron 25.0%
! CTTCAAAAATGTAATTAGGT+TGG - chr7.4:64016928-64016947 None:intergenic 25.0%
!! CAACCTAATTACATTTTTGA+AGG + chr7.4:64016926-64016945 MS.gene87162:CDS 25.0%
!!! TGTAAAAGCATTATATGTGT+AGG - chr7.4:64016342-64016361 None:intergenic 25.0%
ATGGAAACAAATTGGTGAAA+TGG + chr7.4:64016654-64016673 MS.gene87162:intron 30.0%
CCAATATAAATGCTGATCAA+TGG + chr7.4:64015999-64016018 MS.gene87162:CDS 30.0%
CCATCTTAAACCAGATATTA+AGG + chr7.4:64016250-64016269 MS.gene87162:CDS 30.0%
CCATTGATCAGCATTTATAT+TGG - chr7.4:64016002-64016021 None:intergenic 30.0%
TAATCATCGTTGAATGTCAT+TGG - chr7.4:64016989-64017008 None:intergenic 30.0%
! AAATTGATTTCCTGCTTCAT+GGG - chr7.4:64017046-64017065 None:intergenic 30.0%
! CCTTAATATCTGGTTTAAGA+TGG - chr7.4:64016253-64016272 None:intergenic 30.0%
! TTGTTTCTTACATTTGAGAC+CGG + chr7.4:64016600-64016619 MS.gene87162:intron 30.0%
! TTTTTATTATGTACTCCCTC+CGG - chr7.4:64016622-64016641 None:intergenic 30.0%
!! AATGGACTACAAAAGAGAAT+AGG + chr7.4:64016017-64016036 MS.gene87162:CDS 30.0%
ACAACATATTTGGAGACAAC+TGG + chr7.4:64016783-64016802 MS.gene87162:CDS 35.0%
ACTACTCCAGAAGATGAATT+TGG + chr7.4:64016953-64016972 MS.gene87162:CDS 35.0%
AGAATGCCAAGAAAAAATCC+AGG + chr7.4:64016882-64016901 MS.gene87162:CDS 35.0%
AGTTGTCTCCAAATATGTTG+TGG - chr7.4:64016784-64016803 None:intergenic 35.0%
ATGGACCTTAAAACAATGCA+TGG + chr7.4:64016217-64016236 MS.gene87162:CDS 35.0%
CAAGAAAAAATCCAGGAACA+TGG + chr7.4:64016889-64016908 MS.gene87162:CDS 35.0%
CTGCTTATGGTAAATGAGAA+CGG - chr7.4:64015936-64015955 None:intergenic 35.0%
GAAATTTCAAGAAGATTGCC+AGG + chr7.4:64016809-64016828 MS.gene87162:CDS 35.0%
GCACATGAATGGAAACAAAT+TGG + chr7.4:64016646-64016665 MS.gene87162:intron 35.0%
GCAGGAAATCAATTTCATGA+AGG + chr7.4:64017051-64017070 MS.gene87162:CDS 35.0%
GTTGTCTCCAAATATGTTGT+GGG - chr7.4:64016783-64016802 None:intergenic 35.0%
TAAAACAATGCATGGAGAGA+TGG + chr7.4:64016225-64016244 MS.gene87162:CDS 35.0%
TAAGCAGCAGAATACTATTC+AGG + chr7.4:64015949-64015968 MS.gene87162:CDS 35.0%
TATCCACCAAATTCATCTTC+TGG - chr7.4:64016962-64016981 None:intergenic 35.0%
TCGATTTAGAAAGAGATCCA+CGG - chr7.4:64016198-64016217 None:intergenic 35.0%
TGCTTATGGTAAATGAGAAC+GGG - chr7.4:64015935-64015954 None:intergenic 35.0%
TGGATCTCTTTCTAAATCGA+TGG + chr7.4:64016198-64016217 MS.gene87162:CDS 35.0%
! AATTTCATGAAGGCCTTATG+TGG + chr7.4:64017061-64017080 MS.gene87162:CDS 35.0%
! AGTTTTGCAACCAGTGAAAT+TGG + chr7.4:64015881-64015900 MS.gene87162:CDS 35.0%
! CTTAATTTTCACCATGTTCC+TGG - chr7.4:64016903-64016922 None:intergenic 35.0%
! GAAATTGATTTCCTGCTTCA+TGG - chr7.4:64017047-64017066 None:intergenic 35.0%
! GAATAGTATTCTGCTGCTTA+TGG - chr7.4:64015949-64015968 None:intergenic 35.0%
!! TCTCTCCATGCATTGTTTTA+AGG - chr7.4:64016225-64016244 None:intergenic 35.0%
!!! CATGTTCCTGGATTTTTTCT+TGG - chr7.4:64016891-64016910 None:intergenic 35.0%
ACATAACTATCGTTACGTCC+TGG - chr7.4:64016830-64016849 None:intergenic 40.0%
ACTCCAGAAGATGAATTTGG+TGG + chr7.4:64016956-64016975 MS.gene87162:CDS 40.0%
GATCAAGCCCACAACATATT+TGG + chr7.4:64016773-64016792 MS.gene87162:CDS 40.0%
TGGTTGCAAAACTAGAGTGA+TGG - chr7.4:64015874-64015893 None:intergenic 40.0%
! TAGCATTTTTGTCGAATCCG+TGG + chr7.4:64016178-64016197 MS.gene87162:intron 40.0%
! TTCTTACATTTGAGACCGGA+GGG + chr7.4:64016604-64016623 MS.gene87162:intron 40.0%
! TTTCTTACATTTGAGACCGG+AGG + chr7.4:64016603-64016622 MS.gene87162:intron 40.0%
!! TGGATTAGAGTTTTATCCCG+AGG + chr7.4:64017018-64017037 MS.gene87162:CDS 40.0%
GTAGAATGAGCCATTGAGTG+AGG + chr7.4:64016733-64016752 MS.gene87162:intron 45.0%
! GGCATTCTCGACTTGCTTAA+TGG - chr7.4:64016870-64016889 None:intergenic 45.0%
! TGAGAACGGGTTCTTGATCA+TGG - chr7.4:64015922-64015941 None:intergenic 45.0%
! TTTTGCTCCTCCTCACTCAA+TGG - chr7.4:64016746-64016765 None:intergenic 45.0%
!!! TTTTTTTTATTTATTTTATG+CGG - chr7.4:64016376-64016395 None:intergenic 5.0%
GAATGAGCCATTGAGTGAGG+AGG + chr7.4:64016736-64016755 MS.gene87162:intron 50.0%
TTCCTGCTTCATGGGAACCT+CGG - chr7.4:64017038-64017057 None:intergenic 50.0%
TCCTGCTTCATGGGAACCTC+GGG - chr7.4:64017037-64017056 None:intergenic 55.0%
TCCCGAGGTTCCCATGAAGC+AGG + chr7.4:64017033-64017052 MS.gene87162:CDS 60.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 64015857 64017093 64015857 ID=MS.gene87162
chr7.4 mRNA 64015857 64017093 64015857 ID=MS.gene87162.t1;Parent=MS.gene87162
chr7.4 exon 64015857 64016038 64015857 ID=MS.gene87162.t1.exon1;Parent=MS.gene87162.t1
chr7.4 CDS 64015857 64016038 64015857 ID=cds.MS.gene87162.t1;Parent=MS.gene87162.t1
chr7.4 exon 64016181 64016271 64016181 ID=MS.gene87162.t1.exon2;Parent=MS.gene87162.t1
chr7.4 CDS 64016181 64016271 64016181 ID=cds.MS.gene87162.t1;Parent=MS.gene87162.t1
chr7.4 exon 64016737 64017093 64016737 ID=MS.gene87162.t1.exon3;Parent=MS.gene87162.t1
chr7.4 CDS 64016737 64017093 64016737 ID=cds.MS.gene87162.t1;Parent=MS.gene87162.t1
Gene Sequence

>MS.gene87162

ATGGATGAGGTTAACCATCACTCTAGTTTTGCAACCAGTGAAATTGGATACAAATATCAAGACCATGATCAAGAACCCGTTCTCATTTACCATAAGCAGCAGAATACTATTCAGGAGAATAATGAAATTCATCACAAATATTCCAATATAAATGCTGATCAATGGACTACAAAAGAGAATAGGTACATAAAATCATTTATAATTTCTATACTTCGTACTACATGTTTTCATATATAGTTTTATTATGGTTCAAGTTCAATATATGTTTAACTCTTTTGTTTTGCAATTTGATTAATCTTATCTTTTTTTTATATATATTTTTAGCATTTTTGTCGAATCCGTGGATCTCTTTCTAAATCGATGGACCTTAAAACAATGCATGGAGAGATGGTACCATCTTAAACCAGATATTAAGGTGTTTCTTTTTAAATCCTTTTTAACACTTATTTTAGCAAAATCCTTTTTATCTTTTATTGCGTAAACCTACACATATAATGCTTTTACATGTACATAAAACCGCATAAAATAAATAAAAAAAATAGAAATGCTTATCAGTTAAATGCATAAATGCAATTGAATCATCATTTGTATATGATTTTATAACAACAAGTGGAATTCGTAGCTAACATTTATTTTATATTCATTGATGCAAGTTCTTATAGATTGTACAATATTGACATAATAGTTTTAGTTATTACATTTCATTGAAAAACCTAATGGATGTGTGTATTTTTTTTTTTTTTTTGTTTCTTACATTTGAGACCGGAGGGAGTACATAATAAAAATATTGCACATGAATGGAAACAAATTGGTGAAATGGAAATCTTCCTCTATTTAATTATATATAGACGTAATCTAAACATGTTTTGTATAATTGTAGAATGAGCCATTGAGTGAGGAGGAGCAAAACATACTAGATCAAGCCCACAACATATTTGGAGACAACTGGACTGAAATTTCAAGAAGATTGCCAGGACGTAACGATAGTTATGTTAAGAATCATTTGAACGCCATTAAGCAAGTCGAGAATGCCAAGAAAAAATCCAGGAACATGGTGAAAATTAAGAACCAACCTAATTACATTTTTGAAGGTTTGACTACTCCAGAAGATGAATTTGGTGGATATGTGCCAATGACATTCAACGATGATTATGAGTTATATGGATTAGAGTTTTATCCCGAGGTTCCCATGAAGCAGGAAATCAATTTCATGAAGGCCTTATGTGGAAACGTTTAA

Protein sequence

>MS.gene87162.t1

MDEVNHHSSFATSEIGYKYQDHDQEPVLIYHKQQNTIQENNEIHHKYSNINADQWTTKENSIFVESVDLFLNRWTLKQCMERWYHLKPDIKNEPLSEEEQNILDQAHNIFGDNWTEISRRLPGRNDSYVKNHLNAIKQVENAKKKSRNMVKIKNQPNYIFEGLTTPEDEFGGYVPMTFNDDYELYGLEFYPEVPMKQEINFMKALCGNV