Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87198.t1 | XP_013445108.1 | 96.1 | 231 | 6 | 1 | 1 | 231 | 1 | 228 | 3.20E-91 | 344.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87198.t1 | O04479 | 55.5 | 220 | 49 | 4 | 2 | 213 | 4 | 182 | 2.9e-56 | 219.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87198.t1 | A0A072TNP9 | 96.1 | 231 | 6 | 1 | 1 | 231 | 1 | 228 | 2.3e-91 | 344.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene87198.t1 | TF | LOB |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87198.t1 | MTR_8g040900 | 97.835 | 231 | 2 | 1 | 1 | 231 | 1 | 228 | 1.28e-168 | 464 |
| MS.gene87198.t1 | MTR_4g105170 | 66.929 | 127 | 42 | 0 | 5 | 131 | 9 | 135 | 4.77e-59 | 184 |
| MS.gene87198.t1 | MTR_3g077240 | 75.221 | 113 | 25 | 1 | 1 | 110 | 1 | 113 | 4.57e-58 | 182 |
| MS.gene87198.t1 | MTR_7g033800 | 69.912 | 113 | 34 | 0 | 2 | 114 | 6 | 118 | 3.17e-57 | 179 |
| MS.gene87198.t1 | MTR_5g017950 | 73.394 | 109 | 29 | 0 | 2 | 110 | 3 | 111 | 9.76e-54 | 175 |
| MS.gene87198.t1 | MTR_6g005070 | 69.643 | 112 | 32 | 1 | 1 | 110 | 1 | 112 | 1.35e-53 | 169 |
| MS.gene87198.t1 | MTR_6g005080 | 68.750 | 112 | 33 | 1 | 1 | 110 | 2 | 113 | 9.32e-53 | 167 |
| MS.gene87198.t1 | MTR_3g452660 | 69.091 | 110 | 34 | 0 | 1 | 110 | 1 | 110 | 8.39e-51 | 167 |
| MS.gene87198.t1 | MTR_3g073690 | 67.568 | 111 | 35 | 1 | 1 | 110 | 2 | 112 | 1.56e-48 | 158 |
| MS.gene87198.t1 | MTR_5g083230 | 63.551 | 107 | 39 | 0 | 5 | 111 | 4 | 110 | 1.60e-46 | 152 |
| MS.gene87198.t1 | MTR_3g031660 | 62.617 | 107 | 40 | 0 | 1 | 107 | 1 | 107 | 7.12e-46 | 150 |
| MS.gene87198.t1 | MTR_4g083680 | 65.347 | 101 | 35 | 0 | 6 | 106 | 14 | 114 | 5.56e-45 | 148 |
| MS.gene87198.t1 | MTR_5g083010 | 61.321 | 106 | 41 | 0 | 4 | 109 | 8 | 113 | 4.44e-43 | 144 |
| MS.gene87198.t1 | MTR_5g083960 | 43.590 | 156 | 88 | 0 | 4 | 159 | 36 | 191 | 2.77e-42 | 142 |
| MS.gene87198.t1 | MTR_2g068760 | 60.784 | 102 | 40 | 0 | 6 | 107 | 12 | 113 | 2.78e-42 | 141 |
| MS.gene87198.t1 | MTR_3g071590 | 59.615 | 104 | 42 | 0 | 4 | 107 | 5 | 108 | 7.94e-42 | 140 |
| MS.gene87198.t1 | MTR_3g071420 | 61.386 | 101 | 39 | 0 | 4 | 104 | 11 | 111 | 5.80e-41 | 138 |
| MS.gene87198.t1 | MTR_3g094690 | 56.604 | 106 | 46 | 0 | 6 | 111 | 46 | 151 | 1.31e-40 | 139 |
| MS.gene87198.t1 | MTR_2g093310 | 54.717 | 106 | 48 | 0 | 6 | 111 | 32 | 137 | 1.31e-38 | 133 |
| MS.gene87198.t1 | MTR_3g094690 | 56.604 | 106 | 45 | 1 | 6 | 111 | 46 | 150 | 1.47e-38 | 134 |
| MS.gene87198.t1 | MTR_5g075020 | 58.491 | 106 | 44 | 0 | 6 | 111 | 42 | 147 | 2.26e-37 | 130 |
| MS.gene87198.t1 | MTR_5g075020 | 58.491 | 106 | 44 | 0 | 6 | 111 | 46 | 151 | 4.61e-37 | 130 |
| MS.gene87198.t1 | MTR_7g096530 | 56.897 | 116 | 42 | 2 | 1 | 108 | 1 | 116 | 5.64e-37 | 129 |
| MS.gene87198.t1 | MTR_4g060950 | 55.340 | 103 | 45 | 1 | 6 | 107 | 23 | 125 | 2.99e-36 | 126 |
| MS.gene87198.t1 | MTR_1g095850 | 57.000 | 100 | 43 | 0 | 8 | 107 | 73 | 172 | 2.40e-35 | 125 |
| MS.gene87198.t1 | MTR_8g079660 | 49.091 | 110 | 56 | 0 | 7 | 116 | 8 | 117 | 1.53e-34 | 124 |
| MS.gene87198.t1 | MTR_7g028905 | 50.485 | 103 | 51 | 0 | 6 | 108 | 4 | 106 | 7.06e-34 | 118 |
| MS.gene87198.t1 | MTR_1g070220 | 52.679 | 112 | 52 | 1 | 3 | 113 | 11 | 122 | 4.80e-33 | 119 |
| MS.gene87198.t1 | MTR_8g036105 | 53.043 | 115 | 53 | 1 | 7 | 120 | 18 | 132 | 1.03e-32 | 118 |
| MS.gene87198.t1 | MTR_8g036085 | 52.830 | 106 | 49 | 1 | 7 | 111 | 23 | 128 | 1.36e-32 | 118 |
| MS.gene87198.t1 | MTR_8g079620 | 46.087 | 115 | 62 | 0 | 7 | 121 | 8 | 122 | 3.52e-30 | 112 |
| MS.gene87198.t1 | MTR_7g074990 | 57.895 | 95 | 39 | 1 | 7 | 100 | 20 | 114 | 6.58e-30 | 111 |
| MS.gene87198.t1 | MTR_4g107450 | 48.000 | 100 | 52 | 0 | 8 | 107 | 11 | 110 | 6.96e-30 | 113 |
| MS.gene87198.t1 | MTR_6g027710 | 49.515 | 103 | 51 | 1 | 6 | 107 | 39 | 141 | 1.74e-29 | 110 |
| MS.gene87198.t1 | MTR_5g093010 | 50.538 | 93 | 46 | 0 | 8 | 100 | 6 | 98 | 3.88e-29 | 107 |
| MS.gene87198.t1 | MTR_5g080470 | 49.505 | 101 | 50 | 1 | 1 | 100 | 1 | 101 | 4.79e-29 | 108 |
| MS.gene87198.t1 | MTR_7g096610 | 53.465 | 101 | 46 | 1 | 1 | 100 | 2 | 102 | 6.59e-28 | 106 |
| MS.gene87198.t1 | MTR_6g011250 | 43.860 | 114 | 56 | 2 | 1 | 113 | 3 | 109 | 9.88e-28 | 104 |
| MS.gene87198.t1 | MTR_6g011200 | 40.000 | 130 | 69 | 3 | 1 | 121 | 3 | 132 | 1.58e-27 | 105 |
| MS.gene87198.t1 | MTR_6g011230 | 42.574 | 101 | 58 | 0 | 4 | 104 | 8 | 108 | 3.26e-27 | 103 |
| MS.gene87198.t1 | MTR_1g070205 | 48.246 | 114 | 56 | 2 | 7 | 119 | 12 | 123 | 4.31e-27 | 104 |
| MS.gene87198.t1 | MTR_7g075230 | 49.550 | 111 | 53 | 2 | 7 | 114 | 12 | 122 | 4.81e-26 | 100 |
| MS.gene87198.t1 | MTR_4g099230 | 40.476 | 126 | 59 | 3 | 8 | 117 | 3 | 128 | 4.87e-26 | 99.4 |
| MS.gene87198.t1 | MTR_8g079580 | 45.455 | 110 | 59 | 1 | 7 | 115 | 8 | 117 | 9.04e-26 | 100 |
| MS.gene87198.t1 | MTR_8g017090 | 40.385 | 104 | 62 | 0 | 8 | 111 | 13 | 116 | 2.54e-25 | 101 |
| MS.gene87198.t1 | MTR_6g027700 | 42.056 | 107 | 61 | 1 | 1 | 106 | 1 | 107 | 1.78e-24 | 96.7 |
| MS.gene87198.t1 | MTR_2g100020 | 39.604 | 101 | 61 | 0 | 8 | 108 | 20 | 120 | 9.00e-24 | 96.7 |
| MS.gene87198.t1 | MTR_5g085390 | 41.228 | 114 | 66 | 1 | 1 | 113 | 3 | 116 | 2.49e-22 | 91.7 |
| MS.gene87198.t1 | MTR_4g088035 | 33.721 | 86 | 57 | 0 | 8 | 93 | 7 | 92 | 1.55e-15 | 73.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87198.t1 | AT1G65620 | 59.483 | 232 | 56 | 5 | 2 | 231 | 4 | 199 | 1.44e-75 | 227 |
| MS.gene87198.t1 | AT1G65620 | 59.483 | 232 | 56 | 5 | 2 | 231 | 4 | 199 | 1.44e-75 | 227 |
| MS.gene87198.t1 | AT1G65620 | 59.483 | 232 | 56 | 5 | 2 | 231 | 4 | 199 | 1.44e-75 | 227 |
| MS.gene87198.t1 | AT1G65620 | 59.483 | 232 | 56 | 5 | 2 | 231 | 4 | 199 | 1.44e-75 | 227 |
| MS.gene87198.t1 | AT1G65620 | 59.483 | 232 | 56 | 5 | 2 | 231 | 4 | 199 | 1.44e-75 | 227 |
| MS.gene87198.t1 | AT5G63090 | 69.291 | 127 | 34 | 2 | 1 | 123 | 1 | 126 | 1.13e-56 | 179 |
| MS.gene87198.t1 | AT5G63090 | 69.291 | 127 | 34 | 2 | 1 | 123 | 1 | 126 | 1.13e-56 | 179 |
| MS.gene87198.t1 | AT5G63090 | 69.291 | 127 | 34 | 2 | 1 | 123 | 1 | 126 | 1.13e-56 | 179 |
| MS.gene87198.t1 | AT5G63090 | 69.291 | 127 | 34 | 2 | 1 | 123 | 1 | 126 | 1.13e-56 | 179 |
| MS.gene87198.t1 | AT5G66870 | 75.455 | 110 | 27 | 0 | 1 | 110 | 1 | 110 | 1.05e-55 | 180 |
| MS.gene87198.t1 | AT3G27650 | 70.093 | 107 | 32 | 0 | 4 | 110 | 36 | 142 | 9.12e-54 | 170 |
| MS.gene87198.t1 | AT2G23660 | 70.192 | 104 | 31 | 0 | 4 | 107 | 2 | 105 | 1.26e-50 | 167 |
| MS.gene87198.t1 | AT2G23660 | 70.192 | 104 | 31 | 0 | 4 | 107 | 2 | 105 | 1.26e-50 | 167 |
| MS.gene87198.t1 | AT2G23660 | 70.192 | 104 | 31 | 0 | 4 | 107 | 2 | 105 | 1.26e-50 | 167 |
| MS.gene87198.t1 | AT2G30130 | 65.049 | 103 | 36 | 0 | 5 | 107 | 6 | 108 | 6.63e-46 | 151 |
| MS.gene87198.t1 | AT1G31320 | 69.318 | 88 | 27 | 0 | 6 | 93 | 12 | 99 | 9.76e-42 | 140 |
| MS.gene87198.t1 | AT2G30340 | 57.692 | 104 | 44 | 0 | 6 | 109 | 52 | 155 | 7.82e-41 | 140 |
| MS.gene87198.t1 | AT2G30340 | 57.692 | 104 | 44 | 0 | 6 | 109 | 51 | 154 | 9.15e-41 | 140 |
| MS.gene87198.t1 | AT1G07900 | 57.547 | 106 | 45 | 0 | 6 | 111 | 32 | 137 | 1.40e-39 | 135 |
| MS.gene87198.t1 | AT3G11090 | 56.731 | 104 | 45 | 0 | 4 | 107 | 8 | 111 | 1.39e-38 | 132 |
| MS.gene87198.t1 | AT1G16530 | 57.798 | 109 | 45 | 1 | 6 | 113 | 13 | 121 | 2.03e-38 | 131 |
| MS.gene87198.t1 | AT2G28500 | 56.604 | 106 | 46 | 0 | 6 | 111 | 54 | 159 | 6.31e-38 | 132 |
| MS.gene87198.t1 | AT2G40470 | 57.547 | 106 | 45 | 0 | 6 | 111 | 19 | 124 | 2.47e-37 | 129 |
| MS.gene87198.t1 | AT2G40470 | 51.200 | 125 | 60 | 1 | 6 | 129 | 44 | 168 | 3.41e-37 | 130 |
| MS.gene87198.t1 | AT3G26660 | 52.679 | 112 | 46 | 1 | 8 | 112 | 6 | 117 | 4.85e-37 | 126 |
| MS.gene87198.t1 | AT3G26620 | 53.333 | 105 | 49 | 0 | 8 | 112 | 6 | 110 | 5.24e-37 | 126 |
| MS.gene87198.t1 | AT3G03760 | 55.340 | 103 | 45 | 1 | 6 | 107 | 50 | 152 | 4.83e-33 | 120 |
| MS.gene87198.t1 | AT4G00210 | 54.630 | 108 | 48 | 1 | 7 | 113 | 11 | 118 | 1.06e-32 | 118 |
| MS.gene87198.t1 | AT3G50510 | 48.182 | 110 | 57 | 0 | 1 | 110 | 6 | 115 | 1.27e-32 | 117 |
| MS.gene87198.t1 | AT3G50510 | 48.182 | 110 | 57 | 0 | 1 | 110 | 6 | 115 | 1.27e-32 | 117 |
| MS.gene87198.t1 | AT2G42430 | 50.893 | 112 | 54 | 1 | 2 | 112 | 10 | 121 | 2.05e-32 | 118 |
| MS.gene87198.t1 | AT5G06080 | 50.450 | 111 | 54 | 1 | 1 | 110 | 1 | 111 | 5.46e-30 | 110 |
| MS.gene87198.t1 | AT2G45410 | 41.722 | 151 | 80 | 2 | 10 | 152 | 19 | 169 | 1.26e-29 | 109 |
| MS.gene87198.t1 | AT2G31310 | 49.091 | 110 | 55 | 1 | 1 | 109 | 1 | 110 | 1.99e-29 | 108 |
| MS.gene87198.t1 | AT2G45420 | 51.456 | 103 | 49 | 1 | 10 | 111 | 40 | 142 | 2.05e-29 | 111 |
| MS.gene87198.t1 | AT4G00220 | 52.427 | 103 | 48 | 1 | 10 | 111 | 20 | 122 | 3.18e-29 | 109 |
| MS.gene87198.t1 | AT5G35900 | 48.113 | 106 | 55 | 0 | 8 | 113 | 6 | 111 | 4.87e-29 | 108 |
| MS.gene87198.t1 | AT3G58190 | 49.038 | 104 | 52 | 1 | 7 | 109 | 11 | 114 | 4.41e-28 | 106 |
| MS.gene87198.t1 | AT2G42440 | 47.273 | 110 | 57 | 1 | 1 | 109 | 1 | 110 | 7.90e-28 | 106 |
| MS.gene87198.t1 | AT3G13850 | 40.952 | 105 | 62 | 0 | 8 | 112 | 37 | 141 | 6.53e-27 | 104 |
| MS.gene87198.t1 | AT3G47870 | 40.000 | 105 | 63 | 0 | 8 | 112 | 37 | 141 | 1.35e-25 | 102 |
| MS.gene87198.t1 | AT1G72980 | 37.838 | 111 | 69 | 0 | 4 | 114 | 10 | 120 | 1.50e-20 | 86.7 |
| MS.gene87198.t1 | AT4G22700 | 40.385 | 104 | 60 | 2 | 8 | 110 | 6 | 108 | 1.96e-19 | 83.2 |
| MS.gene87198.t1 | AT1G06280 | 39.080 | 87 | 53 | 0 | 8 | 94 | 25 | 111 | 1.63e-18 | 80.9 |
| MS.gene87198.t1 | AT2G19820 | 32.584 | 89 | 59 | 1 | 6 | 93 | 11 | 99 | 3.76e-14 | 67.4 |
| MS.gene87198.t1 | AT5G15060 | 39.726 | 73 | 42 | 1 | 8 | 78 | 18 | 90 | 1.14e-13 | 66.2 |
| MS.gene87198.t1 | AT3G27940 | 42.424 | 66 | 38 | 0 | 7 | 72 | 5 | 70 | 5.19e-11 | 59.7 |
| MS.gene87198.t1 | AT3G27940 | 42.424 | 66 | 38 | 0 | 7 | 72 | 5 | 70 | 5.19e-11 | 59.7 |
| MS.gene87198.t1 | AT3G27940 | 42.424 | 66 | 38 | 0 | 7 | 72 | 5 | 70 | 5.19e-11 | 59.7 |
Find 48 sgRNAs with CRISPR-Local
Find 56 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGACTTCGGGATCCTGTTTA+TGG | 0.160354 | 8.4:-58297633 | MS.gene87198:CDS |
| TCAACATCAACAACATAATT+TGG | 0.253618 | 8.4:-58297331 | MS.gene87198:CDS |
| CACATTCTGGTTGGCATTTG+CGG | 0.315598 | 8.4:+58297808 | None:intergenic |
| GTGCTAAATCCGAACTTTCT+CGG | 0.374243 | 8.4:-58297548 | MS.gene87198:CDS |
| TAAGGTGCAAATGCACATTC+TGG | 0.376608 | 8.4:+58297795 | None:intergenic |
| GGCTGATCAGGTGGAAAATA+AGG | 0.377629 | 8.4:+58297777 | None:intergenic |
| TGAAGCTGAGATGCGACTTC+GGG | 0.381214 | 8.4:-58297646 | MS.gene87198:CDS |
| ATGAATATTTCTGCTAGCTT+AGG | 0.398380 | 8.4:-58297255 | MS.gene87198:CDS |
| ATCGCAGCAGCAGCTACTGC+CGG | 0.415834 | 8.4:-58297510 | MS.gene87198:CDS |
| GCCGGTCATGGACTCATAAC+CGG | 0.444703 | 8.4:-58297492 | MS.gene87198:CDS |
| CATAGTGCTGCTGGTGGTGG+TGG | 0.453028 | 8.4:-58297210 | MS.gene87198:CDS |
| TGTTGTTGATGTTGATCCCT+TGG | 0.455156 | 8.4:+58297339 | None:intergenic |
| ACACCAACACAACCATAAAC+AGG | 0.455574 | 8.4:+58297621 | None:intergenic |
| CCAACTTCGTCAGCTTCAAA+TGG | 0.466283 | 8.4:-58297580 | MS.gene87198:CDS |
| ATGAAGCTGAGATGCGACTT+CGG | 0.470470 | 8.4:-58297647 | MS.gene87198:CDS |
| CTTCAACATCATAGTGCTGC+TGG | 0.473475 | 8.4:-58297219 | MS.gene87198:CDS |
| GAATCTGTCACGGCCGCTGC+AGG | 0.483621 | 8.4:-58297459 | MS.gene87198:CDS |
| CAACTATGATGCTAGTCTCT+TGG | 0.489577 | 8.4:-58297280 | MS.gene87198:CDS |
| GGACTCATAACCGGCATGAC+AGG | 0.499561 | 8.4:-58297483 | MS.gene87198:CDS |
| GCAGCAGCTACTGCCGGTCA+TGG | 0.500625 | 8.4:-58297504 | MS.gene87198:CDS |
| CATCATAGTGCTGCTGGTGG+TGG | 0.501541 | 8.4:-58297213 | MS.gene87198:CDS |
| GCTGACGAAGTTGGTGTTGA+AGG | 0.501764 | 8.4:+58297589 | None:intergenic |
| GCCGGTTATGAGTCCATGAC+CGG | 0.516685 | 8.4:+58297491 | None:intergenic |
| CCATTTGAAGCTGACGAAGT+TGG | 0.517758 | 8.4:+58297580 | None:intergenic |
| ACGCAGAACACTGCCGGTGG+TGG | 0.528599 | 8.4:-58297423 | MS.gene87198:CDS |
| TGCTGCACATGGTGAATTTG+AGG | 0.533620 | 8.4:+58297845 | None:intergenic |
| GACAGATTCACCTGTCATGC+CGG | 0.536335 | 8.4:+58297473 | None:intergenic |
| GCTAGGAGCGGTTAACAGTA+CGG | 0.540067 | 8.4:+58297178 | None:intergenic |
| CACCACACGCAGAACACTGC+CGG | 0.541631 | 8.4:-58297429 | MS.gene87198:CDS |
| GATCCTGTTTATGGTTGTGT+TGG | 0.542374 | 8.4:-58297624 | MS.gene87198:CDS |
| CAGCATCTTCACGTTGGTGA+GGG | 0.561735 | 8.4:+58297685 | None:intergenic |
| ACAGCATCTTCACGTTGGTG+AGG | 0.580209 | 8.4:+58297684 | None:intergenic |
| CACCGGCAGTGTTCTGCGTG+TGG | 0.589919 | 8.4:+58297427 | None:intergenic |
| GTGCAAATGCACATTCTGGT+TGG | 0.592305 | 8.4:+58297799 | None:intergenic |
| GTTACTGTTATTACCACCAC+CGG | 0.593385 | 8.4:+58297410 | None:intergenic |
| CGGCAGTGTTCTGCGTGTGG+TGG | 0.633171 | 8.4:+58297430 | None:intergenic |
| TGGTGAATTTGAGGATGCCA+TGG | 0.633685 | 8.4:+58297854 | None:intergenic |
| AGAAACTTGCATGCTGCACA+TGG | 0.636607 | 8.4:+58297834 | None:intergenic |
| GGTGGTGGTAATAACAGTAA+CGG | 0.644161 | 8.4:-58297408 | MS.gene87198:CDS |
| CATGACAGGTGAATCTGTCA+CGG | 0.646689 | 8.4:-58297469 | MS.gene87198:CDS |
| CACACGCAGAACACTGCCGG+TGG | 0.663015 | 8.4:-58297426 | MS.gene87198:CDS |
| AATTAACAGCATCTTCACGT+TGG | 0.680722 | 8.4:+58297679 | None:intergenic |
| GTGGTGGTAATTACCTGCAG+CGG | 0.695355 | 8.4:+58297446 | None:intergenic |
| TTAGAAACCCTAGCTAGGAG+CGG | 0.696119 | 8.4:+58297166 | None:intergenic |
| GATGTTGATAACGATGGTCA+CGG | 0.703058 | 8.4:+58297385 | None:intergenic |
| CAACATCATAGTGCTGCTGG+TGG | 0.709794 | 8.4:-58297216 | MS.gene87198:CDS |
| GATGATGATGTTGATAACGA+TGG | 0.724184 | 8.4:+58297379 | None:intergenic |
| AGGAGCGGTTAACAGTACGG+AGG | 0.803339 | 8.4:+58297181 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATTTGGTAAGAAATTTTGA+TGG | - | chr8.4:58297716-58297735 | MS.gene87198:CDS | 20.0% |
| ! | TCAACATCAACAACATAATT+TGG | - | chr8.4:58297700-58297719 | MS.gene87198:CDS | 25.0% |
| !! | GCAAATGTTCATAGAATTTT+TGG | - | chr8.4:58297287-58297306 | MS.gene87198:CDS | 25.0% |
| !! | TGAACATTTGCAAATTTTTG+TGG | + | chr8.4:58297278-58297297 | None:intergenic | 25.0% |
| ! | ATGAATATTTCTGCTAGCTT+AGG | - | chr8.4:58297776-58297795 | MS.gene87198:CDS | 30.0% |
| !! | TTGGTAAGAAATTTTGATGG+TGG | - | chr8.4:58297719-58297738 | MS.gene87198:CDS | 30.0% |
| !!! | AACATCATCAGTTTTTTCCA+AGG | - | chr8.4:58297675-58297694 | MS.gene87198:CDS | 30.0% |
| !!! | ACATCATCAGTTTTTTCCAA+GGG | - | chr8.4:58297676-58297695 | MS.gene87198:CDS | 30.0% |
| !!! | AGAAATTTTGATGGTGGAAT+TGG | - | chr8.4:58297725-58297744 | MS.gene87198:CDS | 30.0% |
| !!! | GAAATTTTGATGGTGGAATT+GGG | - | chr8.4:58297726-58297745 | MS.gene87198:CDS | 30.0% |
| AATTAACAGCATCTTCACGT+TGG | + | chr8.4:58297355-58297374 | None:intergenic | 35.0% | |
| !! | GATGATGATGTTGATAACGA+TGG | + | chr8.4:58297655-58297674 | None:intergenic | 35.0% |
| !!! | GTTTTGAAACCGAGAAAGTT+CGG | + | chr8.4:58297495-58297514 | None:intergenic | 35.0% |
| ACACCAACACAACCATAAAC+AGG | + | chr8.4:58297413-58297432 | None:intergenic | 40.0% | |
| GATCCTGTTTATGGTTGTGT+TGG | - | chr8.4:58297407-58297426 | MS.gene87198:CDS | 40.0% | |
| GTGCTAAATCCGAACTTTCT+CGG | - | chr8.4:58297483-58297502 | MS.gene87198:CDS | 40.0% | |
| GTTACTGTTATTACCACCAC+CGG | + | chr8.4:58297624-58297643 | None:intergenic | 40.0% | |
| TAAGGTGCAAATGCACATTC+TGG | + | chr8.4:58297239-58297258 | None:intergenic | 40.0% | |
| TGTTGTTGATGTTGATCCCT+TGG | + | chr8.4:58297695-58297714 | None:intergenic | 40.0% | |
| ! | AATTTTTGTGGCTGATCAGG+TGG | + | chr8.4:58297266-58297285 | None:intergenic | 40.0% |
| ! | CAACTATGATGCTAGTCTCT+TGG | - | chr8.4:58297751-58297770 | MS.gene87198:CDS | 40.0% |
| ! | GCAAATTTTTGTGGCTGATC+AGG | + | chr8.4:58297269-58297288 | None:intergenic | 40.0% |
| ! | GGTGGTGGTAATAACAGTAA+CGG | - | chr8.4:58297623-58297642 | MS.gene87198:CDS | 40.0% |
| !! | GATGTTGATAACGATGGTCA+CGG | + | chr8.4:58297649-58297668 | None:intergenic | 40.0% |
| AGAAACTTGCATGCTGCACA+TGG | + | chr8.4:58297200-58297219 | None:intergenic | 45.0% | |
| ATGAAGCTGAGATGCGACTT+CGG | - | chr8.4:58297384-58297403 | MS.gene87198:CDS | 45.0% | |
| CATGACAGGTGAATCTGTCA+CGG | - | chr8.4:58297562-58297581 | MS.gene87198:CDS | 45.0% | |
| CCAACTTCGTCAGCTTCAAA+TGG | - | chr8.4:58297451-58297470 | MS.gene87198:CDS | 45.0% | |
| CTTCAACATCATAGTGCTGC+TGG | - | chr8.4:58297812-58297831 | MS.gene87198:CDS | 45.0% | |
| GGCTGATCAGGTGGAAAATA+AGG | + | chr8.4:58297257-58297276 | None:intergenic | 45.0% | |
| GTGCAAATGCACATTCTGGT+TGG | + | chr8.4:58297235-58297254 | None:intergenic | 45.0% | |
| TGCTGCACATGGTGAATTTG+AGG | + | chr8.4:58297189-58297208 | None:intergenic | 45.0% | |
| ! | CACATTCTGGTTGGCATTTG+CGG | + | chr8.4:58297226-58297245 | None:intergenic | 45.0% |
| ! | CCATTTGAAGCTGACGAAGT+TGG | + | chr8.4:58297454-58297473 | None:intergenic | 45.0% |
| ACAGCATCTTCACGTTGGTG+AGG | + | chr8.4:58297350-58297369 | None:intergenic | 50.0% | |
| CGACTTCGGGATCCTGTTTA+TGG | - | chr8.4:58297398-58297417 | MS.gene87198:CDS | 50.0% | |
| GACAGATTCACCTGTCATGC+CGG | + | chr8.4:58297561-58297580 | None:intergenic | 50.0% | |
| GTGGTGGTAATTACCTGCAG+CGG | + | chr8.4:58297588-58297607 | None:intergenic | 50.0% | |
| TGAAGCTGAGATGCGACTTC+GGG | - | chr8.4:58297385-58297404 | MS.gene87198:CDS | 50.0% | |
| ! | CAACATCATAGTGCTGCTGG+TGG | - | chr8.4:58297815-58297834 | MS.gene87198:CDS | 50.0% |
| ! | CAGCATCTTCACGTTGGTGA+GGG | + | chr8.4:58297349-58297368 | None:intergenic | 50.0% |
| !! | GCTGACGAAGTTGGTGTTGA+AGG | + | chr8.4:58297445-58297464 | None:intergenic | 50.0% |
| AGGAGCGGTTAACAGTACGG+AGG | + | chr8.4:58297853-58297872 | None:intergenic | 55.0% | |
| GCCGGTCATGGACTCATAAC+CGG | - | chr8.4:58297539-58297558 | MS.gene87198:CDS | 55.0% | |
| GCCGGTTATGAGTCCATGAC+CGG | + | chr8.4:58297543-58297562 | None:intergenic | 55.0% | |
| GGACTCATAACCGGCATGAC+AGG | - | chr8.4:58297548-58297567 | MS.gene87198:CDS | 55.0% | |
| ! | CATCATAGTGCTGCTGGTGG+TGG | - | chr8.4:58297818-58297837 | MS.gene87198:CDS | 55.0% |
| ATCGCAGCAGCAGCTACTGC+CGG | - | chr8.4:58297521-58297540 | MS.gene87198:CDS | 60.0% | |
| CACCACACGCAGAACACTGC+CGG | - | chr8.4:58297602-58297621 | MS.gene87198:CDS | 60.0% | |
| !! | CATAGTGCTGCTGGTGGTGG+TGG | - | chr8.4:58297821-58297840 | MS.gene87198:CDS | 60.0% |
| ACGCAGAACACTGCCGGTGG+TGG | - | chr8.4:58297608-58297627 | MS.gene87198:CDS | 65.0% | |
| CACACGCAGAACACTGCCGG+TGG | - | chr8.4:58297605-58297624 | MS.gene87198:CDS | 65.0% | |
| CACCGGCAGTGTTCTGCGTG+TGG | + | chr8.4:58297607-58297626 | None:intergenic | 65.0% | |
| CGGCAGTGTTCTGCGTGTGG+TGG | + | chr8.4:58297604-58297623 | None:intergenic | 65.0% | |
| GAATCTGTCACGGCCGCTGC+AGG | - | chr8.4:58297572-58297591 | MS.gene87198:CDS | 65.0% | |
| GCAGCAGCTACTGCCGGTCA+TGG | - | chr8.4:58297527-58297546 | MS.gene87198:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 58297179 | 58297874 | 58297179 | ID=MS.gene87198 |
| chr8.4 | mRNA | 58297179 | 58297874 | 58297179 | ID=MS.gene87198.t1;Parent=MS.gene87198 |
| chr8.4 | exon | 58297179 | 58297874 | 58297179 | ID=MS.gene87198.t1.exon1;Parent=MS.gene87198.t1 |
| chr8.4 | CDS | 58297179 | 58297874 | 58297179 | ID=cds.MS.gene87198.t1;Parent=MS.gene87198.t1 |
| Gene Sequence |
| Protein sequence |
