AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene87253

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene87253.t1 MTR_3g450850 66.327 294 87 3 1 290 1 286 1.93e-127 384
MS.gene87253.t1 MTR_4g035820 42.537 268 137 5 45 306 68 324 2.24e-58 211
MS.gene87253.t1 MTR_4g035820 46.875 96 51 0 439 534 326 421 3.43e-20 95.9
MS.gene87253.t1 MTR_4g071400 70.968 124 33 1 594 717 1 121 2.83e-53 180
MS.gene87253.t1 MTR_3g012160 87.097 93 12 0 199 291 1 93 2.84e-48 165
MS.gene87253.t1 MTR_1g070355 34.510 255 150 8 45 296 69 309 1.96e-31 130
MS.gene87253.t1 MTR_6g051425 29.286 280 185 5 55 329 18 289 1.73e-30 123
MS.gene87253.t1 MTR_5g076070 27.119 236 161 5 57 290 56 282 4.21e-23 100
MS.gene87253.t1 MTR_5g083630 67.241 58 19 0 200 257 19 76 4.12e-19 84.0
MS.gene87253.t1 MTR_6g014355 37.662 154 79 4 133 277 14 159 3.68e-18 85.1
MS.gene87253.t1 MTR_3g095940 72.000 50 13 1 508 556 94 143 1.44e-15 75.1
MS.gene87253.t1 MTR_0558s0020 26.336 262 169 7 59 302 41 296 2.56e-14 74.7
MS.gene87253.t1 MTR_6g022080 37.681 138 71 6 66 198 97 224 1.00e-13 72.4
MS.gene87253.t1 MTR_7g446520 27.165 254 148 8 60 305 94 318 6.73e-13 71.2
MS.gene87253.t1 MTR_1g019850 31.757 148 72 6 37 177 55 180 8.65e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene87253.t1 AT1G10240 49.524 630 307 4 48 672 42 665 0.0 646
MS.gene87253.t1 AT1G10240 49.524 630 307 4 48 672 42 665 0.0 646
MS.gene87253.t1 AT5G28530 43.259 675 339 11 25 678 28 679 0.0 545
MS.gene87253.t1 AT5G28530 43.259 675 339 11 25 678 28 679 0.0 545
MS.gene87253.t1 AT5G28530 43.259 675 343 11 25 678 28 683 0.0 543
MS.gene87253.t1 AT5G28530 38.647 665 321 12 25 678 28 616 3.52e-147 445
MS.gene87253.t1 AT5G28530 38.647 665 325 12 25 678 28 620 8.19e-147 444
MS.gene87253.t1 AT4G38180 30.836 574 379 7 54 617 73 638 1.14e-84 285
MS.gene87253.t1 AT4G15090 28.716 693 443 16 54 721 51 717 4.03e-82 280
MS.gene87253.t1 AT4G15090 28.716 693 443 16 54 721 51 717 4.03e-82 280
MS.gene87253.t1 AT4G15090 28.716 693 443 16 54 721 51 717 4.03e-82 280
MS.gene87253.t1 AT4G15090 28.716 693 443 16 54 721 51 717 4.03e-82 280
MS.gene87253.t1 AT4G15090 28.716 693 443 16 54 721 51 717 4.03e-82 280
MS.gene87253.t1 AT1G76320 28.673 565 361 11 60 610 3 539 2.54e-66 233
MS.gene87253.t1 AT3G22170 25.547 685 429 20 54 705 70 706 2.83e-66 236
MS.gene87253.t1 AT3G22170 25.547 685 429 20 54 705 70 706 2.83e-66 236
MS.gene87253.t1 AT1G76320 28.673 565 361 11 60 610 3 539 3.43e-66 234
MS.gene87253.t1 AT1G76320 28.673 565 361 11 60 610 3 539 3.43e-66 234
MS.gene87253.t1 AT1G76320 28.673 565 361 11 60 610 3 539 4.21e-66 233
MS.gene87253.t1 AT5G18960 26.227 591 364 13 39 616 198 729 1.84e-59 216
MS.gene87253.t1 AT5G18960 26.227 591 364 13 39 616 198 729 1.84e-59 216
MS.gene87253.t1 AT3G06250 25.997 577 352 12 54 616 190 705 2.10e-58 213
MS.gene87253.t1 AT3G06250 25.997 577 352 12 54 616 190 705 2.10e-58 213
MS.gene87253.t1 AT3G06250 25.997 577 352 12 54 616 190 705 2.10e-58 213
MS.gene87253.t1 AT2G27110 25.896 614 403 13 54 658 49 619 1.34e-55 206
MS.gene87253.t1 AT2G27110 25.896 614 403 13 54 658 49 619 1.34e-55 206
MS.gene87253.t1 AT2G27110 28.637 433 291 9 235 658 51 474 7.82e-51 190
MS.gene87253.t1 AT4G19990 26.451 586 354 14 56 614 23 558 2.75e-49 186
MS.gene87253.t1 AT4G19990 26.451 586 354 14 56 614 23 558 2.75e-49 186
MS.gene87253.t1 AT4G19990 26.451 586 354 14 56 614 23 558 3.41e-49 186
MS.gene87253.t1 AT4G19990 26.451 586 354 14 56 614 23 558 3.41e-49 186
MS.gene87253.t1 AT4G19990 26.451 586 354 14 56 614 23 558 3.75e-49 186
MS.gene87253.t1 AT4G19990 26.451 586 354 14 56 614 37 572 3.77e-49 186
MS.gene87253.t1 AT1G52520 25.514 584 381 16 53 613 84 636 6.80e-49 184
MS.gene87253.t1 AT4G38170 27.027 481 318 10 267 726 45 513 5.94e-46 173
MS.gene87253.t1 AT1G80010 24.470 613 362 18 54 613 95 659 2.83e-45 174
MS.gene87253.t1 AT4G19990 26.248 541 337 12 86 614 11 501 4.83e-45 172
MS.gene87253.t1 AT2G32250 26.772 381 261 4 246 615 180 553 5.05e-45 173
MS.gene87253.t1 AT2G32250 26.772 381 261 4 246 615 139 512 7.15e-45 173
MS.gene87253.t1 AT2G32250 26.772 381 261 4 246 615 180 553 7.26e-45 173
MS.gene87253.t1 AT2G32250 26.772 381 261 4 246 615 180 553 7.26e-45 173
MS.gene87253.t1 AT2G32250 26.772 381 261 4 246 615 180 553 7.91e-45 173
MS.gene87253.t1 AT2G32250 26.772 381 261 4 246 615 180 553 7.91e-45 173
MS.gene87253.t1 AT2G32250 26.772 381 261 4 246 615 180 553 7.91e-45 173
MS.gene87253.t1 AT2G32250 26.772 381 261 4 246 615 139 512 8.26e-45 173
MS.gene87253.t1 AT3G59470 37.273 110 61 3 54 163 70 171 1.50e-13 71.6
MS.gene87253.t1 AT3G59470 37.273 110 61 3 54 163 70 171 1.50e-13 71.6
MS.gene87253.t1 AT3G59470 37.273 110 61 3 54 163 82 183 2.06e-13 71.6

Find 107 sgRNAs with CRISPR-Local

Find 152 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAAGATAATTTGATTTATT+TGG 0.093166 2.2:-14550105 MS.gene87253:CDS
TCTACTCTCATCGTCAATTT+TGG 0.121901 2.2:-14550908 MS.gene87253:CDS
TACAGGTAACTTACCATTTC+TGG 0.139830 2.2:-14551680 MS.gene87253:CDS
TTGACTATATGACTGCATTT+TGG 0.141368 2.2:+14550269 None:intergenic
ACATCATAACCATCATTATT+TGG 0.153794 2.2:+14550062 None:intergenic
CTAAAGGCTTTGAAGGAAAA+TGG 0.187093 2.2:-14551882 MS.gene87253:CDS
TTCATTCAGATGGGCTTTAA+AGG 0.197395 2.2:-14551326 MS.gene87253:intron
ACAAACTAGAGCATATTATT+TGG 0.199763 2.2:-14551519 MS.gene87253:CDS
ACAATCAGAACTTACTTATT+AGG 0.237831 2.2:+14549861 None:intergenic
TGAACGAGAATGGGAATTAA+TGG 0.241089 2.2:-14550973 MS.gene87253:CDS
ATCAAATTATCTTGACTTGC+AGG 0.274591 2.2:+14550113 None:intergenic
GAGCATATTATTTGGGCATT+TGG 0.276151 2.2:-14551511 MS.gene87253:CDS
AGTACCATAATTCTCTAATT+AGG 0.289351 2.2:-14550616 MS.gene87253:CDS
ATTCGAGCATATGAATCTTT+TGG 0.301566 2.2:-14551481 MS.gene87253:CDS
TTGTCCTAATTAGAGAATTA+TGG 0.303893 2.2:+14550612 None:intergenic
TGATTGGTTCCGCCGCAATT+TGG 0.306685 2.2:+14549942 None:intergenic
GTGACAACGTCGAAGCTTCT+TGG 0.321956 2.2:+14549968 None:intergenic
AATCATCTATAGATTCTAAA+TGG 0.332504 2.2:+14550997 None:intergenic
GAATTAATGGTTGCACAATT+TGG 0.333830 2.2:-14550960 MS.gene87253:CDS
TTTGCAGCATGTTCCTCAAT+AGG 0.334456 2.2:+14550584 None:intergenic
GCAGCTTCAACTCCACCTTT+TGG 0.341287 2.2:+14549990 None:intergenic
AAATTCCTCCGACAGATAAA+AGG 0.354413 2.2:-14550140 MS.gene87253:CDS
CATATAGTCAAATGCAATAA+TGG 0.354919 2.2:-14550257 MS.gene87253:CDS
ACTTTAAACAGGAGTTTCAT+AGG 0.356852 2.2:-14551025 MS.gene87253:CDS
CTAATATCCTTCTCCAGAAA+TGG 0.365717 2.2:+14551667 None:intergenic
TGCCACTTGGAATGTGGATT+GGG 0.366378 2.2:-14551411 MS.gene87253:CDS
CAAACTAGAGCATATTATTT+GGG 0.370107 2.2:-14551518 MS.gene87253:CDS
TCAGTCTCTAATTCGATGTT+TGG 0.382120 2.2:-14550219 MS.gene87253:CDS
TTGGTTTCTAAAGGCTTTGA+AGG 0.384106 2.2:-14551889 MS.gene87253:CDS
GAGATATATTGACTTTAAAC+AGG 0.399521 2.2:-14551036 MS.gene87253:CDS
TTTGCTGGGATGACGACAAC+TGG 0.403764 2.2:-14550858 MS.gene87253:CDS
ATGTAAAAGTCTGAAAGATA+TGG 0.416400 2.2:-14551581 MS.gene87253:CDS
TAAAGGCTTTGAAGGAAAAT+GGG 0.419353 2.2:-14551881 MS.gene87253:CDS
TCATGAACAGTACTAGTTCC+AGG 0.424449 2.2:+14552177 None:intergenic
AAATACATTGTAAAATTCAT+AGG 0.433492 2.2:+14552110 None:intergenic
TTTGGAACATCGGGTACATT+GGG 0.437206 2.2:+14550080 None:intergenic
TTGGGGCCTAGCTTATTTGA+AGG 0.438270 2.2:-14550889 MS.gene87253:CDS
GCAAAGTTGTTGGTTTCTAA+AGG 0.440350 2.2:-14551898 MS.gene87253:CDS
GAACTAGTACTGTTCATGAA+TGG 0.450674 2.2:-14552173 MS.gene87253:CDS
CCATGAGTTATTAGATGATA+AGG 0.455510 2.2:-14551830 MS.gene87253:CDS
TGGATTAACTCAAACGCATA+TGG 0.457746 2.2:+14550551 None:intergenic
CCATCATTATTTGGAACATC+GGG 0.457944 2.2:+14550071 None:intergenic
ACAATTCCGCGAGTCATGAT+TGG 0.463296 2.2:+14549926 None:intergenic
TTGTTGCAAAGTTGTCTAGT+TGG 0.463573 2.2:-14551079 MS.gene87253:CDS
TAGACGACCACCATCAACTT+TGG 0.465253 2.2:+14550457 None:intergenic
TAGTTGTAGCGCAAAGTTGT+TGG 0.465803 2.2:-14551908 MS.gene87253:CDS
CTTTCTCTTCTCCATCGAAA+AGG 0.467284 2.2:+14550302 None:intergenic
AGAAGAAATCTTCATTCAGA+TGG 0.469575 2.2:-14551336 MS.gene87253:CDS
AACTAGTACTGTTCATGAAT+GGG 0.472834 2.2:-14552172 MS.gene87253:CDS
AAATATGCTGCCATTCATAC+AGG 0.475969 2.2:-14550506 MS.gene87253:CDS
TTAGAGAAATCAATAGATAC+AGG 0.477570 2.2:-14551697 MS.gene87253:CDS
CGACATCATACCAAAGTTGA+TGG 0.478069 2.2:-14550467 MS.gene87253:CDS
ATTTGGAACATCGGGTACAT+TGG 0.482649 2.2:+14550079 None:intergenic
TAAGAAATAGTGCTAGTGAT+TGG 0.489143 2.2:-14552293 MS.gene87253:CDS
ATAATTGTTACCTGTATGAA+TGG 0.491038 2.2:+14550496 None:intergenic
AAATCCAAAGGTCGGCCAAA+AGG 0.496241 2.2:-14550020 MS.gene87253:CDS
TGGATAGGATCACAAAGGGT+TGG 0.496334 2.2:-14549774 MS.gene87253:intron
CCAAAAGGTTCAAGGCCAAA+AGG 0.499841 2.2:-14550005 MS.gene87253:CDS
GAGTCTGAAGATAATCCATC+AGG 0.500480 2.2:-14552030 MS.gene87253:CDS
ATATTATTGTTATCAAAAGC+AGG 0.503849 2.2:-14551754 MS.gene87253:CDS
ATGCCACTTGGAATGTGGAT+TGG 0.511770 2.2:-14551412 MS.gene87253:CDS
ACCATCATTATTTGGAACAT+CGG 0.512682 2.2:+14550070 None:intergenic
TGATCCGTAAGGATTGTTTG+TGG 0.512910 2.2:+14551173 None:intergenic
GAATTTGTTTGTCATCGAGC+TGG 0.517742 2.2:-14551994 MS.gene87253:CDS
CCAGGCTGAGGTTCTTGATG+TGG 0.518896 2.2:+14552195 None:intergenic
TGGATTGGGGTTGATAATCA+TGG 0.518964 2.2:-14551397 MS.gene87253:CDS
CCCGATGTTCCAAATAATGA+TGG 0.520874 2.2:-14550071 MS.gene87253:CDS
ATTTGGCAATTCCTTTGCAA+TGG 0.524413 2.2:+14551130 None:intergenic
CATCTAATAACTCATGGTTG+TGG 0.528510 2.2:+14551836 None:intergenic
TAGACAATTCAATCTCATGT+TGG 0.528559 2.2:+14550531 None:intergenic
CTTTAAACAGGAGTTTCATA+GGG 0.528837 2.2:-14551024 MS.gene87253:CDS
AAGGTCGGCCAAAAGGTTCA+AGG 0.530766 2.2:-14550013 MS.gene87253:CDS
TAACTTACCATTTCTGGAGA+AGG 0.534997 2.2:-14551674 MS.gene87253:CDS
TTCCAAGTGGCATATCATAA+CGG 0.535191 2.2:+14551422 None:intergenic
AATAGTCCTTCAAATAAGCT+AGG 0.536211 2.2:+14550883 None:intergenic
ATGGTGGTCGTCTAGTAAGC+TGG 0.537867 2.2:-14550448 MS.gene87253:CDS
CCACATCAAGAACCTCAGCC+TGG 0.539330 2.2:-14552195 MS.gene87253:CDS
CCTTATCATCTAATAACTCA+TGG 0.545462 2.2:+14551830 None:intergenic
ATATCCACAAACAATCCTTA+CGG 0.547845 2.2:-14551177 MS.gene87253:CDS
AACCGTTATGATATGCCACT+TGG 0.555362 2.2:-14551424 MS.gene87253:CDS
CGTTGTCACGTTCCAAATTG+CGG 0.556435 2.2:-14549954 MS.gene87253:CDS
TTCTTGGATAGGATCACAAA+GGG 0.557354 2.2:-14549778 MS.gene87253:intron
GAAGAAATCTTCATTCAGAT+GGG 0.559436 2.2:-14551335 MS.gene87253:CDS
ATTGGGATGTTACGATAAGC+GGG 0.561005 2.2:+14551793 None:intergenic
TAGACATCAAGCAGGAGAAG+AGG 0.565504 2.2:-14550654 MS.gene87253:CDS
GTCAAAATTAGACATCAAGC+AGG 0.568777 2.2:-14550662 MS.gene87253:CDS
TGCACAATTTGGTCTAAGCA+AGG 0.581324 2.2:-14550949 MS.gene87253:CDS
TTGTTTCAAAATCCAAAGGT+CGG 0.583293 2.2:-14550028 MS.gene87253:CDS
CCTGTTGTTTCAAAATCCAA+AGG 0.583885 2.2:-14550032 MS.gene87253:CDS
AATTGGGATGTTACGATAAG+CGG 0.587373 2.2:+14551792 None:intergenic
ATCATTAAAGACAAAAGAGA+GGG 0.596933 2.2:-14550189 MS.gene87253:CDS
GTGCAAGATCTTGATCCGTA+AGG 0.602894 2.2:+14551162 None:intergenic
ATGATATGCCACTTGGAATG+TGG 0.611489 2.2:-14551417 MS.gene87253:CDS
ACAGTACTAGTTCCAGGCTG+AGG 0.619979 2.2:+14552183 None:intergenic
TGAGTTATTAGATGATAAGG+AGG 0.622448 2.2:-14551827 MS.gene87253:CDS
AGGCTTTGAAGGAAAATGGG+TGG 0.631084 2.2:-14551878 MS.gene87253:CDS
AAAGGTTCAAGGCCAAAAGG+TGG 0.631199 2.2:-14550002 MS.gene87253:CDS
AATCATTAAAGACAAAAGAG+AGG 0.632966 2.2:-14550190 MS.gene87253:CDS
TCAATATATCTCGAACCCAA+CGG 0.634921 2.2:+14551047 None:intergenic
TACTTATTAGGAGATTGAGA+AGG 0.635742 2.2:+14549873 None:intergenic
GCCACTTGGAATGTGGATTG+GGG 0.646236 2.2:-14551410 MS.gene87253:CDS
GTTCTTGGATAGGATCACAA+AGG 0.649286 2.2:-14549779 MS.gene87253:intron
ACCCCAATCCACATTCCAAG+TGG 0.654914 2.2:+14551409 None:intergenic
ACTTACAGAAGCCATTGCAA+AGG 0.657210 2.2:-14551141 MS.gene87253:CDS
GCGGAACCAATCATGACTCG+CGG 0.676119 2.2:-14549932 MS.gene87253:CDS
CATCATACCAAAGTTGATGG+TGG 0.683172 2.2:-14550464 MS.gene87253:CDS
TGTCACGTTCCAAATTGCGG+CGG 0.703136 2.2:-14549951 MS.gene87253:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATTAACTATTTGATTTTA+TGG - chr2.2:14552252-14552271 MS.gene87253:CDS 10.0%
!! AAATACATTGTAAAATTCAT+AGG + chr2.2:14549946-14549965 None:intergenic 15.0%
!! TAATTAATATATTTGTGAGT+AGG - chr2.2:14551357-14551376 MS.gene87253:CDS 15.0%
!!! TCAAGATAATTTGATTTATT+TGG - chr2.2:14551948-14551967 MS.gene87253:CDS 15.0%
!!! TCAATATTTTTCTTTTTGTT+TGG + chr2.2:14552153-14552172 None:intergenic 15.0%
!!! TTATTTTAGTAACTTGTTAT+AGG - chr2.2:14551302-14551321 MS.gene87253:intron 15.0%
!! AATCATCTATAGATTCTAAA+TGG + chr2.2:14551059-14551078 None:intergenic 20.0%
!! ATATTATTGTTATCAAAAGC+AGG - chr2.2:14550299-14550318 MS.gene87253:CDS 20.0%
!! TTAAAAATTTGTCCTTCAAA+AGG + chr2.2:14549909-14549928 None:intergenic 20.0%
!!! TAATCATTTTTTTGTTAGAC+AGG + chr2.2:14550785-14550804 None:intergenic 20.0%
! AAGTGAAAAATCAAAGGAAA+CGG - chr2.2:14550108-14550127 MS.gene87253:CDS 25.0%
! AATCATTAAAGACAAAAGAG+AGG - chr2.2:14551863-14551882 MS.gene87253:CDS 25.0%
! AATGTATTTGCACGAAAAAA+TGG - chr2.2:14549957-14549976 MS.gene87253:CDS 25.0%
! ACAAACTAGAGCATATTATT+TGG - chr2.2:14550534-14550553 MS.gene87253:CDS 25.0%
! ACAATCAGAACTTACTTATT+AGG + chr2.2:14552195-14552214 None:intergenic 25.0%
! ACATCATAACCATCATTATT+TGG + chr2.2:14551994-14552013 None:intergenic 25.0%
! AGTACCATAATTCTCTAATT+AGG - chr2.2:14551437-14551456 MS.gene87253:CDS 25.0%
! ATAATTGTTACCTGTATGAA+TGG + chr2.2:14551560-14551579 None:intergenic 25.0%
! ATCATTAAAGACAAAAGAGA+GGG - chr2.2:14551864-14551883 MS.gene87253:CDS 25.0%
! ATGTAAAAGTCTGAAAGATA+TGG - chr2.2:14550472-14550491 MS.gene87253:CDS 25.0%
! CAAACTAGAGCATATTATTT+GGG - chr2.2:14550535-14550554 MS.gene87253:CDS 25.0%
! CATATAGTCAAATGCAATAA+TGG - chr2.2:14551796-14551815 MS.gene87253:CDS 25.0%
! TAATATATTTGTGAGTAGGT+TGG - chr2.2:14551361-14551380 MS.gene87253:CDS 25.0%
! TTAGAGAAATCAATAGATAC+AGG - chr2.2:14550356-14550375 MS.gene87253:CDS 25.0%
! TTGATGAAGTGAAAAATCAA+AGG - chr2.2:14550102-14550121 MS.gene87253:CDS 25.0%
! TTGTCCTAATTAGAGAATTA+TGG + chr2.2:14551444-14551463 None:intergenic 25.0%
!! AATTTTATTCAATCACACAG+TGG - chr2.2:14550410-14550429 MS.gene87253:CDS 25.0%
!! AGTTTTCTAACTTTTGTTGA+AGG - chr2.2:14550850-14550869 MS.gene87253:CDS 25.0%
!! CAAAATGCATGTTTTGTATT+TGG + chr2.2:14550943-14550962 None:intergenic 25.0%
!! CATGGAAATTCAATTTTCTT+TGG - chr2.2:14550674-14550693 MS.gene87253:CDS 25.0%
!!! CTATTTGATTTTATGGTTCT+TGG - chr2.2:14552259-14552278 MS.gene87253:CDS 25.0%
!!! GAAAAAATGGATTTTCGATT+AGG - chr2.2:14549970-14549989 MS.gene87253:CDS 25.0%
!!! GAAAAGGAAGATATTTTGAT+GGG + chr2.2:14551738-14551757 None:intergenic 25.0%
!!! GAGATATATTGACTTTAAAC+AGG - chr2.2:14551017-14551036 MS.gene87253:CDS 25.0%
!!! TGTAAAGAATTTGAGTTTTC+TGG - chr2.2:14551658-14551677 MS.gene87253:CDS 25.0%
!!! TTTGACTGATTTTGTAAATC+AGG - chr2.2:14551272-14551291 MS.gene87253:intron 25.0%
AACTAGTACTGTTCATGAAT+GGG - chr2.2:14549881-14549900 MS.gene87253:CDS 30.0%
AATAGTCCTTCAAATAAGCT+AGG + chr2.2:14551173-14551192 None:intergenic 30.0%
ACTTTAAACAGGAGTTTCAT+AGG - chr2.2:14551028-14551047 MS.gene87253:CDS 30.0%
AGAAGAAATCTTCATTCAGA+TGG - chr2.2:14550717-14550736 MS.gene87253:intron 30.0%
ATATCCACAAACAATCCTTA+CGG - chr2.2:14550876-14550895 MS.gene87253:CDS 30.0%
CCATGAGTTATTAGATGATA+AGG - chr2.2:14550223-14550242 MS.gene87253:CDS 30.0%
CCTTATCATCTAATAACTCA+TGG + chr2.2:14550226-14550245 None:intergenic 30.0%
CTTTAAACAGGAGTTTCATA+GGG - chr2.2:14551029-14551048 MS.gene87253:CDS 30.0%
GAAGAAATCTTCATTCAGAT+GGG - chr2.2:14550718-14550737 MS.gene87253:intron 30.0%
GAATTAATGGTTGCACAATT+TGG - chr2.2:14551093-14551112 MS.gene87253:CDS 30.0%
TAAGAAATAGTGCTAGTGAT+TGG - chr2.2:14549760-14549779 MS.gene87253:CDS 30.0%
TACTTATTAGGAGATTGAGA+AGG + chr2.2:14552183-14552202 None:intergenic 30.0%
TAGACAATTCAATCTCATGT+TGG + chr2.2:14551525-14551544 None:intergenic 30.0%
TGAGTTATTAGATGATAAGG+AGG - chr2.2:14550226-14550245 MS.gene87253:CDS 30.0%
TTGTTTCAAAATCCAAAGGT+CGG - chr2.2:14552025-14552044 MS.gene87253:CDS 30.0%
! ACCATCATTATTTGGAACAT+CGG + chr2.2:14551986-14552005 None:intergenic 30.0%
! AGATGATTTTGAACGAGAAT+GGG - chr2.2:14551071-14551090 MS.gene87253:CDS 30.0%
! ATTCGAGCATATGAATCTTT+TGG - chr2.2:14550572-14550591 MS.gene87253:CDS 30.0%
! CAAAACATGCATTTTGCATA+TGG - chr2.2:14550947-14550966 MS.gene87253:CDS 30.0%
! CAAAATATCTTCCTTTTCGA+TGG - chr2.2:14551740-14551759 MS.gene87253:CDS 30.0%
! GTTATAGGTACGTTGTATTT+TGG - chr2.2:14551317-14551336 MS.gene87253:intron 30.0%
! TAGATGATTTTGAACGAGAA+TGG - chr2.2:14551070-14551089 MS.gene87253:CDS 30.0%
! TCTCTTTTATAAACTCCTGA+TGG + chr2.2:14550041-14550060 None:intergenic 30.0%
! TGACTATATGACTGCATTTT+GGG + chr2.2:14551786-14551805 None:intergenic 30.0%
! TGATTTTATGGTTCTTGGAT+AGG - chr2.2:14552264-14552283 MS.gene87253:CDS 30.0%
! TTGACTATATGACTGCATTT+TGG + chr2.2:14551787-14551806 None:intergenic 30.0%
!! ATACGATTTTGATCGTCAAT+TGG + chr2.2:14550281-14550300 None:intergenic 30.0%
!! ATCAAATTATCTTGACTTGC+AGG + chr2.2:14551943-14551962 None:intergenic 30.0%
!! CGAAAAGGAAGATATTTTGA+TGG + chr2.2:14551739-14551758 None:intergenic 30.0%
!! TAAAGGCTTTGAAGGAAAAT+GGG - chr2.2:14550172-14550191 MS.gene87253:CDS 30.0%
!! TACGATTTTGATCGTCAATT+GGG + chr2.2:14550280-14550299 None:intergenic 30.0%
!!! ATATGAATCTTTTGGTGATG+TGG - chr2.2:14550580-14550599 MS.gene87253:CDS 30.0%
!!! TGAAGGACTATTTTTTTGCT+GGG - chr2.2:14551181-14551200 MS.gene87253:CDS 30.0%
!!! TTGAAGGACTATTTTTTTGC+TGG - chr2.2:14551180-14551199 MS.gene87253:CDS 30.0%
AAATATGCTGCCATTCATAC+AGG - chr2.2:14551547-14551566 MS.gene87253:CDS 35.0%
AAATTCCTCCGACAGATAAA+AGG - chr2.2:14551913-14551932 MS.gene87253:CDS 35.0%
AATTGGGATGTTACGATAAG+CGG + chr2.2:14550264-14550283 None:intergenic 35.0%
ATTTGGCAATTCCTTTGCAA+TGG + chr2.2:14550926-14550945 None:intergenic 35.0%
CATCTAATAACTCATGGTTG+TGG + chr2.2:14550220-14550239 None:intergenic 35.0%
CTAATATCCTTCTCCAGAAA+TGG + chr2.2:14550389-14550408 None:intergenic 35.0%
GAACTAGTACTGTTCATGAA+TGG - chr2.2:14549880-14549899 MS.gene87253:CDS 35.0%
GAGCATATTATTTGGGCATT+TGG - chr2.2:14550542-14550561 MS.gene87253:CDS 35.0%
GTCAAAATTAGACATCAAGC+AGG - chr2.2:14551391-14551410 MS.gene87253:CDS 35.0%
TACAGGTAACTTACCATTTC+TGG - chr2.2:14550373-14550392 MS.gene87253:CDS 35.0%
TCAATATATCTCGAACCCAA+CGG + chr2.2:14551009-14551028 None:intergenic 35.0%
TCAGTCTCTAATTCGATGTT+TGG - chr2.2:14551834-14551853 MS.gene87253:CDS 35.0%
TGAACGAGAATGGGAATTAA+TGG - chr2.2:14551080-14551099 MS.gene87253:CDS 35.0%
TGGATTAACTCAAACGCATA+TGG + chr2.2:14551505-14551524 None:intergenic 35.0%
TTCCAAGTGGCATATCATAA+CGG + chr2.2:14550634-14550653 None:intergenic 35.0%
TTCTTGGATAGGATCACAAA+GGG - chr2.2:14552275-14552294 MS.gene87253:CDS 35.0%
TTGTTGCAAAGTTGTCTAGT+TGG - chr2.2:14550974-14550993 MS.gene87253:CDS 35.0%
! CCATCATTATTTGGAACATC+GGG + chr2.2:14551985-14552004 None:intergenic 35.0%
! CTACTCTCATCGTCAATTTT+GGG - chr2.2:14551146-14551165 MS.gene87253:CDS 35.0%
! TAACTTACCATTTCTGGAGA+AGG - chr2.2:14550379-14550398 MS.gene87253:CDS 35.0%
! TACTCTCATCGTCAATTTTG+GGG - chr2.2:14551147-14551166 MS.gene87253:CDS 35.0%
! TAGGACAACTTTTCCTATTG+AGG - chr2.2:14551456-14551475 MS.gene87253:CDS 35.0%
! TCTACTCTCATCGTCAATTT+TGG - chr2.2:14551145-14551164 MS.gene87253:CDS 35.0%
! TTCATTCAGATGGGCTTTAA+AGG - chr2.2:14550727-14550746 MS.gene87253:intron 35.0%
! TTGGTTTCTAAAGGCTTTGA+AGG - chr2.2:14550164-14550183 MS.gene87253:CDS 35.0%
! TTTTACAAGTAGCACTCGAA+TGG + chr2.2:14551701-14551720 None:intergenic 35.0%
!! CCTGTTGTTTCAAAATCCAA+AGG - chr2.2:14552021-14552040 MS.gene87253:CDS 35.0%
!! CTAAAGGCTTTGAAGGAAAA+TGG - chr2.2:14550171-14550190 MS.gene87253:CDS 35.0%
!! GCAAAGTTGTTGGTTTCTAA+AGG - chr2.2:14550155-14550174 MS.gene87253:CDS 35.0%
!!! AGTTGGTTTTCTTTTCCGTT+GGG - chr2.2:14550991-14551010 MS.gene87253:CDS 35.0%
!!! CCTTTGGATTTTGAAACAAC+AGG + chr2.2:14552024-14552043 None:intergenic 35.0%
!!! TAGTTGGTTTTCTTTTCCGT+TGG - chr2.2:14550990-14551009 MS.gene87253:CDS 35.0%
AACCGTTATGATATGCCACT+TGG - chr2.2:14550629-14550648 MS.gene87253:CDS 40.0%
ACTTACAGAAGCCATTGCAA+AGG - chr2.2:14550912-14550931 MS.gene87253:CDS 40.0%
ATTGGGATGTTACGATAAGC+GGG + chr2.2:14550263-14550282 None:intergenic 40.0%
CCCGATGTTCCAAATAATGA+TGG - chr2.2:14551982-14552001 MS.gene87253:CDS 40.0%
CGACATCATACCAAAGTTGA+TGG - chr2.2:14551586-14551605 MS.gene87253:CDS 40.0%
CTTTCTCTTCTCCATCGAAA+AGG + chr2.2:14551754-14551773 None:intergenic 40.0%
GAGTCTGAAGATAATCCATC+AGG - chr2.2:14550023-14550042 MS.gene87253:CDS 40.0%
GTTCTTGGATAGGATCACAA+AGG - chr2.2:14552274-14552293 MS.gene87253:CDS 40.0%
TAGTTGTAGCGCAAAGTTGT+TGG - chr2.2:14550145-14550164 MS.gene87253:CDS 40.0%
TCATGAACAGTACTAGTTCC+AGG + chr2.2:14549879-14549898 None:intergenic 40.0%
TGATCCGTAAGGATTGTTTG+TGG + chr2.2:14550883-14550902 None:intergenic 40.0%
TGCACAATTTGGTCTAAGCA+AGG - chr2.2:14551104-14551123 MS.gene87253:CDS 40.0%
TTTGCAGCATGTTCCTCAAT+AGG + chr2.2:14551472-14551491 None:intergenic 40.0%
! ATGATATGCCACTTGGAATG+TGG - chr2.2:14550636-14550655 MS.gene87253:CDS 40.0%
! ATTTGGAACATCGGGTACAT+TGG + chr2.2:14551977-14551996 None:intergenic 40.0%
! GAATTTGTTTGTCATCGAGC+TGG - chr2.2:14550059-14550078 MS.gene87253:CDS 40.0%
! TTTGGAACATCGGGTACATT+GGG + chr2.2:14551976-14551995 None:intergenic 40.0%
!! CATCATACCAAAGTTGATGG+TGG - chr2.2:14551589-14551608 MS.gene87253:CDS 40.0%
!! CATGAATGGGTTCCTTTTGA+AGG - chr2.2:14549894-14549913 MS.gene87253:CDS 40.0%
!! TGGATTGGGGTTGATAATCA+TGG - chr2.2:14550656-14550675 MS.gene87253:CDS 40.0%
AAAGGTTCAAGGCCAAAAGG+TGG - chr2.2:14552051-14552070 MS.gene87253:CDS 45.0%
AAATCCAAAGGTCGGCCAAA+AGG - chr2.2:14552033-14552052 MS.gene87253:CDS 45.0%
ACAATTCCGCGAGTCATGAT+TGG + chr2.2:14552130-14552149 None:intergenic 45.0%
CCAAAAGGTTCAAGGCCAAA+AGG - chr2.2:14552048-14552067 MS.gene87253:CDS 45.0%
CGTTGTCACGTTCCAAATTG+CGG - chr2.2:14552099-14552118 MS.gene87253:CDS 45.0%
GTGCAAGATCTTGATCCGTA+AGG + chr2.2:14550894-14550913 None:intergenic 45.0%
TAGACATCAAGCAGGAGAAG+AGG - chr2.2:14551399-14551418 MS.gene87253:CDS 45.0%
TAGACGACCACCATCAACTT+TGG + chr2.2:14551599-14551618 None:intergenic 45.0%
TGGATAGGATCACAAAGGGT+TGG - chr2.2:14552279-14552298 MS.gene87253:CDS 45.0%
TTGGGGCCTAGCTTATTTGA+AGG - chr2.2:14551164-14551183 MS.gene87253:CDS 45.0%
! ATGCCACTTGGAATGTGGAT+TGG - chr2.2:14550641-14550660 MS.gene87253:CDS 45.0%
! CAGGCATGCCTTTTATCTGT+CGG + chr2.2:14551924-14551943 None:intergenic 45.0%
! TGCCACTTGGAATGTGGATT+GGG - chr2.2:14550642-14550661 MS.gene87253:CDS 45.0%
!! AGGCTTTGAAGGAAAATGGG+TGG - chr2.2:14550175-14550194 MS.gene87253:CDS 45.0%
!!! CCTTTTGGCCTTGAACCTTT+TGG + chr2.2:14552051-14552070 None:intergenic 45.0%
AAGGTCGGCCAAAAGGTTCA+AGG - chr2.2:14552040-14552059 MS.gene87253:CDS 50.0%
ACAGTACTAGTTCCAGGCTG+AGG + chr2.2:14549873-14549892 None:intergenic 50.0%
ACCCCAATCCACATTCCAAG+TGG + chr2.2:14550647-14550666 None:intergenic 50.0%
ATGGTGGTCGTCTAGTAAGC+TGG - chr2.2:14551605-14551624 MS.gene87253:CDS 50.0%
GTGACAACGTCGAAGCTTCT+TGG + chr2.2:14552088-14552107 None:intergenic 50.0%
TGTCACGTTCCAAATTGCGG+CGG - chr2.2:14552102-14552121 MS.gene87253:CDS 50.0%
TTTGCTGGGATGACGACAAC+TGG - chr2.2:14551195-14551214 MS.gene87253:CDS 50.0%
! GCAGCTTCAACTCCACCTTT+TGG + chr2.2:14552066-14552085 None:intergenic 50.0%
! GCATGCCTTTTATCTGTCGG+AGG + chr2.2:14551921-14551940 None:intergenic 50.0%
! GCCACTTGGAATGTGGATTG+GGG - chr2.2:14550643-14550662 MS.gene87253:CDS 50.0%
! TGAACCTTTTGGCCGACCTT+TGG + chr2.2:14552040-14552059 None:intergenic 50.0%
! TGATTGGTTCCGCCGCAATT+TGG + chr2.2:14552114-14552133 None:intergenic 50.0%
CCACATCAAGAACCTCAGCC+TGG - chr2.2:14549858-14549877 MS.gene87253:intron 55.0%
CCAGGCTGAGGTTCTTGATG+TGG + chr2.2:14549861-14549880 None:intergenic 55.0%
GCGGAACCAATCATGACTCG+CGG - chr2.2:14552121-14552140 MS.gene87253:CDS 55.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 14549747 14552328 14549747 ID=MS.gene87253
chr2.2 mRNA 14549747 14552328 14549747 ID=MS.gene87253.t1;Parent=MS.gene87253
chr2.2 exon 14551327 14552328 14551327 ID=MS.gene87253.t1.exon1;Parent=MS.gene87253.t1
chr2.2 CDS 14551327 14552328 14551327 ID=cds.MS.gene87253.t1;Parent=MS.gene87253.t1
chr2.2 exon 14550782 14551225 14550782 ID=MS.gene87253.t1.exon2;Parent=MS.gene87253.t1
chr2.2 CDS 14550782 14551225 14550782 ID=cds.MS.gene87253.t1;Parent=MS.gene87253.t1
chr2.2 exon 14549876 14550696 14549876 ID=MS.gene87253.t1.exon3;Parent=MS.gene87253.t1
chr2.2 CDS 14549876 14550696 14549876 ID=cds.MS.gene87253.t1;Parent=MS.gene87253.t1
chr2.2 exon 14549747 14549789 14549747 ID=MS.gene87253.t1.exon4;Parent=MS.gene87253.t1
chr2.2 CDS 14549747 14549789 14549747 ID=cds.MS.gene87253.t1;Parent=MS.gene87253.t1
Gene Sequence

>MS.gene87253

ATGACATCTGAAGTAAGAAATAGTGCTAGTGATTGGCACTTAGAGCTTCTTAAAGATTACACTTCTACATATGAAGATGATGAATATAGAAATTCATTTAGCTTTCAAGAGCCACATCAAGAACCTCAGCCTGGAACTAGTACTGTTCATGAATGGGTTCCTTTTGAAGGACAAATTTTTAACACTGATGATGATGCCTATGAATTTTACAATGTATTTGCACGAAAAAATGGATTTTCGATTAGGCGTGATCGCATTTGCAAATCTCATAAAAATGAGTCTGAAGATAATCCATCAGGAGTTTATAAAAGAGAATTTGTTTGTCATCGAGCTGGTATTGTTAAGCAACGCAAAGTTGATGAAGTGAAAAATCAAAGGAAACGGAAATCTTCGCGATGTAGTTGTAGCGCAAAGTTGTTGGTTTCTAAAGGCTTTGAAGGAAAATGGGTGGTTAAATATTTTAATAATAACCACAACCATGAGTTATTAGATGATAAGGAGGTTCAATTTCTTCCCGCTTATCGTAACATCCCAATTGACGATCAAAATCGTATATTATTGTTATCAAAAGCAGGTTGCTCTGTGAGTCTCATAATGAGAGTGCTTGAGTTAGAGAAATCAATAGATACAGGTAACTTACCATTTCTGGAGAAGGATATTAGAAATTTTATTCAATCACACAGTGGTATTGATAAAGAAAATGATGCTTCAGATGTTCTCAAATTATGTAAAAGTCTGAAAGATATGGATAGTGCATTTGAATATGATTTCACAATAGATGAAAGTAACAAACTAGAGCATATTATTTGGGCATTTGGTGATTCAATTCGAGCATATGAATCTTTTGGTGATGTGGTTGTTTTCGATACTACATATCGAATAAACCGTTATGATATGCCACTTGGAATGTGGATTGGGGTTGATAATCATGGAAATTCAATTTTCTTTGGCTGTGTTCTATTGCGAAATGAGAAGAAATCTTCATTCAGATGGGCTTTAAAGGTAAAATTATATTTACATGATAATTTTGTATCTCCTGTCTAACAAAAAAATGATTATTATGTTTATAATAACATTAAAAAAATGATTATTTTCTATTTCAGAGTTTTCTAACTTTTGTTGAAGGAAAATATCCACAAACAATCCTTACGGATCAAGATCTTGCACTTACAGAAGCCATTGCAAAGGAATTGCCAAATACAAAACATGCATTTTGCATATGGCATATTGTTGCAAAGTTGTCTAGTTGGTTTTCTTTTCCGTTGGGTTCGAGATATATTGACTTTAAACAGGAGTTTCATAGGGTGTACCATTTAGAATCTATAGATGATTTTGAACGAGAATGGGAATTAATGGTTGCACAATTTGGTCTAAGCAAGGACGATCATATTGACTTGCTCTACTCTCATCGTCAATTTTGGGGCCTAGCTTATTTGAAGGACTATTTTTTTGCTGGGATGACGACAACTGGACGTTCTGAATCAATCAACTCGTATGTAAAAAGATTCTTAGATGTGAAAACAAGTTTGACTGATTTTGTAAATCAGGTATGAACTTATTTTAGTAACTTGTTATAGGTACGTTGTATTTTGGAAATTGAAACTTACATGTAATTAATATATTTGTGAGTAGGTTGGTGTTGCTGTCAAAATTAGACATCAAGCAGGAGAAGAGGCAAGAATGCGTCAAAAGTACCATAATTCTCTAATTAGGACAACTTTTCCTATTGAGGAACATGCTGCAAAAATACTCACTCCATATGCGTTTGAGTTAATCCAACATGAGATTGAATTGTCTACAAAATATGCTGCCATTCATACAGGTAACAATTATATCGTGCGACATCATACCAAAGTTGATGGTGGTCGTCTAGTAAGCTGGATAGAAGAAGAAGAATCAATTTCTTGTTCTTGTAAAGAATTTGAGTTTTCTGGAATATTGTGCAGACATGCCATTCGAGTGCTACTTGTAAAAAATTATTTTCACTTCCCATCAAAATATCTTCCTTTTCGATGGAGAAGAGAAAGTTCATTGATCCCAAAATGCAGTCATATAGTCAAATGCAATAATGGTTCTCATGTTGAGTTTCAGTCTCTAATTCGATGTTTGGAAGTTGAATCATTAAAGACAAAAGAGAGGGAACAAGTTGCTACTGCAGAGTTAGAGAAATTCCTCCGACAGATAAAAGGCATGCCTGCAAGTCAAGATAATTTGATTTATTTGGAACCCAATGTACCCGATGTTCCAAATAATGATGGTTATGATGTTGAAAATCCTGTTGTTTCAAAATCCAAAGGTCGGCCAAAAGGTTCAAGGCCAAAAGGTGGAGTTGAAGCTGCCAAGAAGCTTCGACGTTGTCACGTTCCAAATTGCGGCGGAACCAATCATGACTCGCGGAATTGTCCAAACAAAAAGAAAAATATTGAAGTATTGCCTTCTCAATCTCCTAATAAGTAAGTTCTGATTGTTTATTTTTAAGTTAGTGTCTAGATTTGTACAATGCTATTATTAACTATTTGATTTTATGGTTCTTGGATAGGATCACAAAGGGTTGGAGACAGTCATCTTCAAATGTGCAATGA
Protein sequence

>MS.gene87253.t1

MTSEVRNSASDWHLELLKDYTSTYEDDEYRNSFSFQEPHQEPQPGTSTVHEWVPFEGQIFNTDDDAYEFYNVFARKNGFSIRRDRICKSHKNESEDNPSGVYKREFVCHRAGIVKQRKVDEVKNQRKRKSSRCSCSAKLLVSKGFEGKWVVKYFNNNHNHELLDDKEVQFLPAYRNIPIDDQNRILLLSKAGCSVSLIMRVLELEKSIDTGNLPFLEKDIRNFIQSHSGIDKENDASDVLKLCKSLKDMDSAFEYDFTIDESNKLEHIIWAFGDSIRAYESFGDVVVFDTTYRINRYDMPLGMWIGVDNHGNSIFFGCVLLRNEKKSSFRWALKSFLTFVEGKYPQTILTDQDLALTEAIAKELPNTKHAFCIWHIVAKLSSWFSFPLGSRYIDFKQEFHRVYHLESIDDFEREWELMVAQFGLSKDDHIDLLYSHRQFWGLAYLKDYFFAGMTTTGRSESINSYVKRFLDVKTSLTDFVNQVGVAVKIRHQAGEEARMRQKYHNSLIRTTFPIEEHAAKILTPYAFELIQHEIELSTKYAAIHTGNNYIVRHHTKVDGGRLVSWIEEEESISCSCKEFEFSGILCRHAIRVLLVKNYFHFPSKYLPFRWRRESSLIPKCSHIVKCNNGSHVEFQSLIRCLEVESLKTKEREQVATAELEKFLRQIKGMPASQDNLIYLEPNVPDVPNNDGYDVENPVVSKSKGRPKGSRPKGGVEAAKKLRRCHVPNCGGTNHDSRNCPNKKKNIEVLPSQSPNKITKGWRQSSSNVQ