AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene87328

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene87328.t1 MTR_8g079620 81.749 263 20 3 1 260 1 238 1.37e-149 417
MS.gene87328.t1 MTR_8g079660 73.664 262 54 2 1 247 1 262 2.18e-137 387
MS.gene87328.t1 MTR_8g079580 48.295 176 83 3 1 173 1 171 1.20e-51 168
MS.gene87328.t1 MTR_5g017950 54.955 111 50 0 8 118 8 118 2.23e-37 134
MS.gene87328.t1 MTR_3g073690 52.727 110 52 0 8 117 9 118 7.85e-37 129
MS.gene87328.t1 MTR_3g452660 42.458 179 73 4 8 165 7 176 1.38e-36 131
MS.gene87328.t1 MTR_4g105170 50.000 104 52 0 8 111 11 114 1.99e-32 117
MS.gene87328.t1 MTR_3g077240 49.038 104 53 0 8 111 10 113 1.70e-31 115
MS.gene87328.t1 MTR_2g093310 44.361 133 69 2 5 135 30 159 5.14e-31 114
MS.gene87328.t1 MTR_7g033800 47.115 104 55 0 8 111 11 114 6.84e-31 113
MS.gene87328.t1 MTR_3g031660 43.564 101 57 0 8 108 7 107 5.63e-30 110
MS.gene87328.t1 MTR_5g083230 45.536 112 59 1 8 117 6 117 1.72e-29 109
MS.gene87328.t1 MTR_8g040900 48.696 115 59 0 8 122 7 121 1.75e-29 111
MS.gene87328.t1 MTR_5g083010 49.020 102 52 0 9 110 12 113 2.93e-29 109
MS.gene87328.t1 MTR_3g071590 45.545 101 55 0 8 108 8 108 4.27e-29 108
MS.gene87328.t1 MTR_2g068760 46.154 104 56 0 5 108 10 113 4.41e-29 108
MS.gene87328.t1 MTR_4g083680 48.000 100 52 0 8 107 15 114 2.27e-28 106
MS.gene87328.t1 MTR_6g005080 43.269 104 59 0 8 111 10 113 3.37e-28 105
MS.gene87328.t1 MTR_6g005070 45.370 108 58 1 8 115 9 115 1.11e-27 103
MS.gene87328.t1 MTR_4g060950 45.714 105 56 1 5 108 21 125 3.25e-27 104
MS.gene87328.t1 MTR_3g094690 44.340 106 59 0 6 111 45 150 1.47e-26 103
MS.gene87328.t1 MTR_1g095850 47.525 101 53 0 9 109 73 173 2.28e-26 103
MS.gene87328.t1 MTR_5g083960 44.231 104 58 0 8 111 39 142 3.02e-26 102
MS.gene87328.t1 MTR_3g094690 45.283 106 57 1 6 111 45 149 7.77e-26 101
MS.gene87328.t1 MTR_3g071420 47.423 97 51 0 9 105 15 111 5.75e-25 98.2
MS.gene87328.t1 MTR_6g027700 44.660 103 56 1 4 105 3 105 6.83e-25 98.6
MS.gene87328.t1 MTR_5g075020 38.235 136 81 1 6 138 41 176 2.44e-24 97.8
MS.gene87328.t1 MTR_6g027710 41.667 108 62 1 2 108 34 141 3.23e-24 97.4
MS.gene87328.t1 MTR_5g075020 41.739 115 67 0 6 120 45 159 3.44e-24 97.4
MS.gene87328.t1 MTR_4g099230 45.098 102 55 1 9 109 3 104 5.46e-24 94.7
MS.gene87328.t1 MTR_8g036085 45.714 105 56 1 8 111 23 127 9.76e-24 96.3
MS.gene87328.t1 MTR_7g028905 37.143 105 66 0 5 109 2 106 1.22e-23 92.8
MS.gene87328.t1 MTR_7g096530 42.718 103 58 1 8 109 14 116 4.73e-23 94.0
MS.gene87328.t1 MTR_5g093010 37.624 101 63 0 9 109 6 106 7.22e-23 91.7
MS.gene87328.t1 MTR_8g017090 37.143 105 66 0 7 111 11 115 9.86e-22 92.4
MS.gene87328.t1 MTR_6g011250 41.346 104 60 1 7 109 8 111 1.10e-21 89.4
MS.gene87328.t1 MTR_7g074990 44.118 102 56 1 8 108 20 121 3.66e-21 89.4
MS.gene87328.t1 MTR_6g011200 40.367 109 61 2 5 109 3 111 6.41e-21 89.0
MS.gene87328.t1 MTR_1g070220 41.584 101 58 1 8 107 15 115 1.36e-20 87.0
MS.gene87328.t1 MTR_4g107450 39.216 102 62 0 7 108 9 110 1.46e-20 89.0
MS.gene87328.t1 MTR_1g070205 43.269 104 58 1 8 110 12 115 1.84e-20 87.4
MS.gene87328.t1 MTR_8g036105 42.718 103 58 1 8 109 18 120 7.95e-20 85.5
MS.gene87328.t1 MTR_7g096610 40.385 104 61 1 8 110 8 111 2.09e-19 84.3
MS.gene87328.t1 MTR_5g085390 40.367 109 61 2 5 109 3 111 5.49e-19 83.6
MS.gene87328.t1 MTR_6g011230 35.354 99 64 0 7 105 10 108 1.06e-18 82.0
MS.gene87328.t1 MTR_5g080470 37.864 103 63 1 9 110 8 110 8.44e-18 79.3
MS.gene87328.t1 MTR_2g100020 33.981 103 68 0 7 109 18 120 1.25e-17 80.5
MS.gene87328.t1 MTR_7g075230 40.952 105 59 2 8 109 12 116 7.48e-17 77.0
MS.gene87328.t1 MTR_4g088035 37.209 86 54 0 9 94 7 92 9.84e-17 77.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene87328.t1 AT5G66870 44.231 156 74 2 8 163 7 149 9.05e-35 127
MS.gene87328.t1 AT1G65620 57.692 104 44 0 8 111 9 112 5.90e-33 119
MS.gene87328.t1 AT1G65620 57.692 104 44 0 8 111 9 112 5.90e-33 119
MS.gene87328.t1 AT1G65620 57.692 104 44 0 8 111 9 112 5.90e-33 119
MS.gene87328.t1 AT1G65620 57.692 104 44 0 8 111 9 112 5.90e-33 119
MS.gene87328.t1 AT1G65620 57.692 104 44 0 8 111 9 112 5.90e-33 119
MS.gene87328.t1 AT5G63090 50.962 104 51 0 8 111 11 114 4.60e-32 116
MS.gene87328.t1 AT5G63090 50.962 104 51 0 8 111 11 114 4.60e-32 116
MS.gene87328.t1 AT5G63090 50.962 104 51 0 8 111 11 114 4.60e-32 116
MS.gene87328.t1 AT5G63090 50.962 104 51 0 8 111 11 114 4.60e-32 116
MS.gene87328.t1 AT2G23660 51.818 110 53 0 8 117 5 114 8.18e-32 119
MS.gene87328.t1 AT2G23660 51.818 110 53 0 8 117 5 114 8.18e-32 119
MS.gene87328.t1 AT2G23660 51.818 110 53 0 8 117 5 114 8.18e-32 119
MS.gene87328.t1 AT1G07900 46.226 106 57 0 3 108 28 133 2.76e-29 109
MS.gene87328.t1 AT3G27650 47.115 104 55 0 8 111 39 142 4.19e-29 108
MS.gene87328.t1 AT2G28500 47.115 104 55 0 5 108 52 155 6.03e-29 110
MS.gene87328.t1 AT2G30130 46.535 101 54 0 8 108 8 108 8.01e-29 108
MS.gene87328.t1 AT1G16530 47.115 104 54 1 6 108 12 115 7.85e-28 105
MS.gene87328.t1 AT1G31320 50.000 88 44 0 8 95 13 100 2.30e-27 103
MS.gene87328.t1 AT3G26660 41.584 101 59 0 9 109 6 106 1.76e-25 97.8
MS.gene87328.t1 AT3G26620 41.584 101 59 0 9 109 6 106 2.16e-25 97.4
MS.gene87328.t1 AT2G30340 43.396 106 60 0 6 111 51 156 2.61e-25 101
MS.gene87328.t1 AT2G30340 43.396 106 60 0 6 111 50 155 3.15e-25 100
MS.gene87328.t1 AT2G40470 42.241 116 67 0 6 121 18 133 3.45e-25 99.4
MS.gene87328.t1 AT3G11090 44.000 100 56 0 9 108 12 111 3.71e-25 98.2
MS.gene87328.t1 AT2G40470 42.241 116 67 0 6 121 43 158 1.22e-24 98.6
MS.gene87328.t1 AT3G50510 42.342 111 63 1 1 111 6 115 1.52e-24 97.4
MS.gene87328.t1 AT3G50510 42.342 111 63 1 1 111 6 115 1.52e-24 97.4
MS.gene87328.t1 AT5G35900 43.269 104 59 0 9 112 6 109 7.06e-24 95.9
MS.gene87328.t1 AT3G47870 39.806 103 62 0 9 111 37 139 1.13e-22 95.1
MS.gene87328.t1 AT1G72980 39.000 100 61 0 9 108 14 113 5.71e-22 91.3
MS.gene87328.t1 AT4G00210 43.689 103 57 1 8 109 11 113 2.16e-21 89.7
MS.gene87328.t1 AT3G03760 43.137 102 57 1 8 108 51 152 2.30e-21 90.9
MS.gene87328.t1 AT2G42430 40.777 103 60 1 8 109 15 117 4.94e-21 89.4
MS.gene87328.t1 AT3G13850 37.864 103 64 0 9 111 37 139 7.45e-21 89.4
MS.gene87328.t1 AT3G58190 42.308 104 59 1 8 110 11 114 2.78e-20 86.7
MS.gene87328.t1 AT2G45420 42.574 101 57 1 11 110 40 140 9.31e-20 86.3
MS.gene87328.t1 AT4G00220 44.000 100 55 1 11 109 20 119 2.81e-19 84.3
MS.gene87328.t1 AT2G42440 38.462 104 63 1 8 110 7 110 2.84e-19 84.3
MS.gene87328.t1 AT2G45410 42.000 100 57 1 11 109 19 118 9.02e-18 79.3
MS.gene87328.t1 AT4G22700 37.302 126 75 4 9 131 6 130 2.57e-17 78.2
MS.gene87328.t1 AT2G31310 36.538 104 65 1 8 110 7 110 1.72e-16 75.9
MS.gene87328.t1 AT5G06080 34.286 105 68 1 8 111 7 111 4.71e-15 71.6
MS.gene87328.t1 AT1G06280 36.709 79 50 0 7 85 23 101 2.49e-13 67.4

Find 96 sgRNAs with CRISPR-Local

Find 97 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATGTTCCTGTTCATGATTC+TGG 0.107641 8.3:+24649783 MS.gene87328:CDS
CTTCTGTGCTTCCTTCTTCT+AGG 0.193943 8.3:+24649844 MS.gene87328:CDS
CTAAAACAAAAGCTTCTTAA+TGG 0.248750 8.3:+24649651 MS.gene87328:CDS
CTCGCCTTTCCTGATTGTTT+CGG 0.296820 8.3:+24649516 MS.gene87328:CDS
ATGTTCCTGTTCATGATTCT+GGG 0.297022 8.3:+24649784 MS.gene87328:CDS
CATCAACAGGAGGAAGCATC+TGG 0.360812 8.3:-24649818 None:intergenic
GTTCATGATTCTGGGTTGGC+TGG 0.362539 8.3:+24649792 MS.gene87328:CDS
AAACGCTCCGGGTTGTCCTT+TGG 0.364971 8.3:-24649243 None:intergenic
GACCTGAGGATTGAGGTATT+TGG 0.365999 8.3:-24649481 None:intergenic
CTACCTTGAAGGTCATTTGC+TGG 0.366573 8.3:-24649411 None:intergenic
GCCAAGGTACACTCGGTGTT+TGG 0.371149 8.3:+24649267 MS.gene87328:CDS
TGTACCTTGGCGAAACGCTC+CGG 0.395772 8.3:-24649255 None:intergenic
GGTGGTTCGGTCGGTGGCAG+TGG 0.408512 8.3:+24649732 MS.gene87328:CDS
TTCTGTGCTTCCTTCTTCTA+GGG 0.409577 8.3:+24649845 MS.gene87328:CDS
GGCTTAAAGCCGAAACAATC+AGG 0.421196 8.3:-24649525 None:intergenic
TGAACAAGCCGTTGAATCCT+TGG 0.427880 8.3:+24649338 MS.gene87328:CDS
CAGGAACATCAGCACCCATC+TGG 0.437219 8.3:-24649770 None:intergenic
AGCACAGAAGGAGCATCAAC+AGG 0.442967 8.3:-24649831 None:intergenic
GGTCGGTGGCAGTGGTGGCT+CGG 0.445795 8.3:+24649740 MS.gene87328:CDS
GACCCTATCCATGGCTGTGC+GGG 0.445984 8.3:+24649387 MS.gene87328:CDS
TCCTGTTCATGATTCTGGGT+TGG 0.449021 8.3:+24649788 MS.gene87328:CDS
CTTCATTCAACCTACCTTGA+AGG 0.452245 8.3:-24649422 None:intergenic
GCCAACCCATCATCACCATC+GGG 0.452840 8.3:-24649675 None:intergenic
AGCTTGGGTGGCACTTCCTA+TGG 0.453993 8.3:-24649200 None:intergenic
GGCTCCTCGGCAATCGACCC+TGG 0.455669 8.3:-24649905 None:intergenic
GGGTTGTCCTTTGGAAAGTA+AGG 0.456967 8.3:-24649234 None:intergenic
CAGGAACATTAACATCCATC+TGG 0.471455 8.3:-24649704 None:intergenic
ATAGTCGGTGGAGAAGCATT+TGG 0.471738 8.3:-24649546 None:intergenic
GATAGTCGGTGGAGGACCAT+TGG 0.472565 8.3:+24649569 MS.gene87328:CDS
TGTTCCTGTTGGTGGTGGTT+CGG 0.474185 8.3:+24649719 MS.gene87328:CDS
GGACCCGCACAGCCATGGAT+AGG 0.477306 8.3:-24649390 None:intergenic
GCCAACCCAGAATCATGAAC+AGG 0.484253 8.3:-24649789 None:intergenic
ATAGTCGGTGGAGGACCATT+GGG 0.490324 8.3:+24649570 MS.gene87328:CDS
TGGTGTATGAAGCTGATGCC+TGG 0.491815 8.3:+24649358 MS.gene87328:CDS
GGTTCGGTCGGTGGCAGTGG+TGG 0.492839 8.3:+24649735 MS.gene87328:CDS
CCGACCGAACCACCACCAAC+AGG 0.493564 8.3:-24649723 None:intergenic
TGCCAACCCATCATCACCAT+CGG 0.501173 8.3:-24649676 None:intergenic
CCTGTTGGTGGTGGTTCGGT+CGG 0.503841 8.3:+24649723 MS.gene87328:CDS
GTTAATGTTCCTGTTGGTGG+TGG 0.504572 8.3:+24649714 MS.gene87328:CDS
TTGCTGGACCCGCACAGCCA+TGG 0.505943 8.3:-24649395 None:intergenic
TAAGGAGCTAAGACACAGCT+TGG 0.506248 8.3:-24649216 None:intergenic
GATGTTAATGTTCCTGTTGG+TGG 0.510237 8.3:+24649711 MS.gene87328:CDS
AATGGCCCCGATGGTGATGA+TGG 0.514770 8.3:+24649669 MS.gene87328:CDS
TAGGTTGAATGAAGCTCAAA+CGG 0.515884 8.3:+24649431 MS.gene87328:CDS
GTACCTTGGCGAAACGCTCC+GGG 0.518769 8.3:-24649254 None:intergenic
CAAGAGTATGAAGAATGAAC+TGG 0.518901 8.3:+24649458 MS.gene87328:CDS
GATGGTGATGATGGGTTGGC+AGG 0.524172 8.3:+24649678 MS.gene87328:CDS
CTAGAAGAAGGAAGCACAGA+AGG 0.524800 8.3:-24649843 None:intergenic
GACCCGCACAGCCATGGATA+GGG 0.530793 8.3:-24649389 None:intergenic
AAAGCATCAGCCCTAGAAGA+AGG 0.535906 8.3:-24649855 None:intergenic
CTTAGCTCCTTACTTTCCAA+AGG 0.539710 8.3:+24649227 MS.gene87328:CDS
ATGAAGCTGATGCCTGGTTG+AGG 0.542839 8.3:+24649364 MS.gene87328:CDS
GTTGGTGGTGGTTCGGTCGG+TGG 0.545494 8.3:+24649726 MS.gene87328:CDS
TGGTTGAGGGACCCTATCCA+TGG 0.545516 8.3:+24649378 MS.gene87328:CDS
CGCCGCAAGCTCAAACGTTG+TGG 0.546240 8.3:-24649926 None:intergenic
TCCAAACACCGAGTGTACCT+TGG 0.546759 8.3:-24649268 None:intergenic
GGTCCAGCAAATGACCTTCA+AGG 0.548176 8.3:+24649408 MS.gene87328:CDS
CCCCGATGGTGATGATGGGT+TGG 0.556104 8.3:+24649674 MS.gene87328:CDS
GGCCAAATACCTCAATCCTC+AGG 0.557788 8.3:+24649479 MS.gene87328:CDS
TGGCTCGGCAAGATTCCAGA+TGG 0.560308 8.3:+24649755 MS.gene87328:CDS
AGATGCTTGCAAGCTGCACA+TGG 0.564745 8.3:-24649177 None:intergenic
TTCATACACCAAGGATTCAA+CGG 0.568271 8.3:-24649346 None:intergenic
TAGCACTAGCACAAGCAACC+AGG 0.569645 8.3:+24649887 MS.gene87328:CDS
TGAAGCTGATGCCTGGTTGA+GGG 0.570610 8.3:+24649365 MS.gene87328:CDS
GGCTCGGCAAGATTCCAGAT+GGG 0.571382 8.3:+24649756 MS.gene87328:CDS
AAGCTTCTTAATGGCCCCGA+TGG 0.576051 8.3:+24649660 MS.gene87328:CDS
ATGGATAGGGTCCCTCAACC+AGG 0.580885 8.3:-24649376 None:intergenic
ATTCAACGACCTGAGGATTG+AGG 0.583762 8.3:-24649488 None:intergenic
GAGCTAAGACACAGCTTGGG+TGG 0.584647 8.3:-24649212 None:intergenic
CAGCTTGCAAGCATCTCCAT+AGG 0.587034 8.3:+24649184 MS.gene87328:CDS
AAGGAGCTAAGACACAGCTT+GGG 0.589356 8.3:-24649215 None:intergenic
ATGGATGTTAATGTTCCTGT+TGG 0.590588 8.3:+24649708 MS.gene87328:CDS
GCTCAAACGTTGTGGCTCCT+CGG 0.592037 8.3:-24649918 None:intergenic
TGAGTCGTTAACTCAGCAGC+TGG 0.600497 8.3:-24649606 None:intergenic
ATCAACAGGAGGAAGCATCT+GGG 0.602324 8.3:-24649817 None:intergenic
GGACCCTATCCATGGCTGTG+CGG 0.609336 8.3:+24649386 MS.gene87328:CDS
GCAACCAGGGTCGATTGCCG+AGG 0.618823 8.3:+24649901 MS.gene87328:CDS
GGCAGCTGATTAAAACCCAA+TGG 0.624529 8.3:-24649585 None:intergenic
CCACCGACTATGATAGTCGG+TGG 0.627702 8.3:+24649558 MS.gene87328:CDS
CCACCGACTATCATAGTCGG+TGG 0.629721 8.3:-24649558 None:intergenic
CAGCAAATGACCTTCAAGGT+AGG 0.629800 8.3:+24649412 MS.gene87328:CDS
GCGTTTCGCCAAGGTACACT+CGG 0.633186 8.3:+24649260 MS.gene87328:CDS
TTACTTTCCAAAGGACAACC+CGG 0.635497 8.3:+24649236 MS.gene87328:CDS
CCTCCACCGACTATCATAGT+CGG 0.638926 8.3:-24649561 None:intergenic
AAAGCCGAAACAATCAGGAA+AGG 0.640067 8.3:-24649520 None:intergenic
CAACCCGGAGCGTTTCGCCA+AGG 0.640771 8.3:+24649251 MS.gene87328:CDS
TCTCCACCGACTATGATAGT+CGG 0.643835 8.3:+24649555 MS.gene87328:CDS
ATGGCCCCGATGGTGATGAT+GGG 0.649484 8.3:+24649670 MS.gene87328:CDS
GATGCTTGCAAGCTGCACAT+GGG 0.660822 8.3:-24649176 None:intergenic
ACAGAAGGAGCATCAACAGG+AGG 0.665410 8.3:-24649828 None:intergenic
AGCACTAGCACAAGCAACCA+GGG 0.667332 8.3:+24649888 MS.gene87328:CDS
AGAACAAATTCAACGACCTG+AGG 0.672940 8.3:-24649495 None:intergenic
TGGGTTGGCAGGATTCCAGA+TGG 0.675884 8.3:+24649689 MS.gene87328:CDS
CCGACTATGATAGTCGGTGG+AGG 0.678129 8.3:+24649561 MS.gene87328:CDS
GGCATCAGCTTCATACACCA+AGG 0.679242 8.3:-24649355 None:intergenic
CCAACCCATCATCACCATCG+GGG 0.738435 8.3:-24649674 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! CTAAAACAAAAGCTTCTTAA+TGG + chr8.3:24649651-24649670 MS.gene87328:CDS 25.0%
GACTAATCTGTTGAAAAAAC+TGG + chr8.3:24649302-24649321 MS.gene87328:CDS 30.0%
!!! GCTTTTGTTTTAGAACTTCT+AGG - chr8.3:24649644-24649663 None:intergenic 30.0%
ATGGATGTTAATGTTCCTGT+TGG + chr8.3:24649708-24649727 MS.gene87328:CDS 35.0%
ATGTTCCTGTTCATGATTCT+GGG + chr8.3:24649784-24649803 MS.gene87328:CDS 35.0%
TTCATACACCAAGGATTCAA+CGG - chr8.3:24649349-24649368 None:intergenic 35.0%
! CAAGAGTATGAAGAATGAAC+TGG + chr8.3:24649458-24649477 MS.gene87328:CDS 35.0%
! TAGGTTGAATGAAGCTCAAA+CGG + chr8.3:24649431-24649450 MS.gene87328:CDS 35.0%
AAAGCCGAAACAATCAGGAA+AGG - chr8.3:24649523-24649542 None:intergenic 40.0%
AGAACAAATTCAACGACCTG+AGG - chr8.3:24649498-24649517 None:intergenic 40.0%
CAGGAACATTAACATCCATC+TGG - chr8.3:24649707-24649726 None:intergenic 40.0%
CTTAGCTCCTTACTTTCCAA+AGG + chr8.3:24649227-24649246 MS.gene87328:CDS 40.0%
CTTCATTCAACCTACCTTGA+AGG - chr8.3:24649425-24649444 None:intergenic 40.0%
GATGTTAATGTTCCTGTTGG+TGG + chr8.3:24649711-24649730 MS.gene87328:CDS 40.0%
GATGTTCCTGTTCATGATTC+TGG + chr8.3:24649783-24649802 MS.gene87328:CDS 40.0%
TTACTTTCCAAAGGACAACC+CGG + chr8.3:24649236-24649255 MS.gene87328:CDS 40.0%
TTCTGTGCTTCCTTCTTCTA+GGG + chr8.3:24649845-24649864 MS.gene87328:CDS 40.0%
AAGGAGCTAAGACACAGCTT+GGG - chr8.3:24649218-24649237 None:intergenic 45.0%
ATTCAACGACCTGAGGATTG+AGG - chr8.3:24649491-24649510 None:intergenic 45.0%
CAGCAAATGACCTTCAAGGT+AGG + chr8.3:24649412-24649431 MS.gene87328:CDS 45.0%
CTACCTTGAAGGTCATTTGC+TGG - chr8.3:24649414-24649433 None:intergenic 45.0%
CTCGCCTTTCCTGATTGTTT+CGG + chr8.3:24649516-24649535 MS.gene87328:CDS 45.0%
CTTCTGTGCTTCCTTCTTCT+AGG + chr8.3:24649844-24649863 MS.gene87328:CDS 45.0%
GGCAGCTGATTAAAACCCAA+TGG - chr8.3:24649588-24649607 None:intergenic 45.0%
GGCTTAAAGCCGAAACAATC+AGG - chr8.3:24649528-24649547 None:intergenic 45.0%
TAAGGAGCTAAGACACAGCT+TGG - chr8.3:24649219-24649238 None:intergenic 45.0%
TCCTGTTCATGATTCTGGGT+TGG + chr8.3:24649788-24649807 MS.gene87328:CDS 45.0%
TGAACAAGCCGTTGAATCCT+TGG + chr8.3:24649338-24649357 MS.gene87328:CDS 45.0%
! AAAGCATCAGCCCTAGAAGA+AGG - chr8.3:24649858-24649877 None:intergenic 45.0%
! AGGGCTGATGCTTTTGACAA+TGG + chr8.3:24649864-24649883 MS.gene87328:CDS 45.0%
! CTAGAAGAAGGAAGCACAGA+AGG - chr8.3:24649846-24649865 None:intergenic 45.0%
! GACCTGAGGATTGAGGTATT+TGG - chr8.3:24649484-24649503 None:intergenic 45.0%
! GGGTTGTCCTTTGGAAAGTA+AGG - chr8.3:24649237-24649256 None:intergenic 45.0%
! GTTAATGTTCCTGTTGGTGG+TGG + chr8.3:24649714-24649733 MS.gene87328:CDS 45.0%
! TCTCCACCGACTATGATAGT+CGG + chr8.3:24649555-24649574 MS.gene87328:CDS 45.0%
!! ATAGTCGGTGGAGAAGCATT+TGG - chr8.3:24649549-24649568 None:intergenic 45.0%
!! ATCAACAGGAGGAAGCATCT+GGG - chr8.3:24649820-24649839 None:intergenic 45.0%
AAGCTTCTTAATGGCCCCGA+TGG + chr8.3:24649660-24649679 MS.gene87328:CDS 50.0%
AGATGCTTGCAAGCTGCACA+TGG - chr8.3:24649180-24649199 None:intergenic 50.0%
AGCACTAGCACAAGCAACCA+GGG + chr8.3:24649888-24649907 MS.gene87328:CDS 50.0%
ATAGTCGGTGGAGGACCATT+GGG + chr8.3:24649570-24649589 MS.gene87328:CDS 50.0%
ATGAAGCTGATGCCTGGTTG+AGG + chr8.3:24649364-24649383 MS.gene87328:CDS 50.0%
CAGCTTGCAAGCATCTCCAT+AGG + chr8.3:24649184-24649203 MS.gene87328:CDS 50.0%
GATGCTTGCAAGCTGCACAT+GGG - chr8.3:24649179-24649198 None:intergenic 50.0%
GCCAACCCAGAATCATGAAC+AGG - chr8.3:24649792-24649811 None:intergenic 50.0%
GGCATCAGCTTCATACACCA+AGG - chr8.3:24649358-24649377 None:intergenic 50.0%
GGCCAAATACCTCAATCCTC+AGG + chr8.3:24649479-24649498 MS.gene87328:CDS 50.0%
GGTCCAGCAAATGACCTTCA+AGG + chr8.3:24649408-24649427 MS.gene87328:CDS 50.0%
TAGCACTAGCACAAGCAACC+AGG + chr8.3:24649887-24649906 MS.gene87328:CDS 50.0%
TCCAAACACCGAGTGTACCT+TGG - chr8.3:24649271-24649290 None:intergenic 50.0%
TGAAGCTGATGCCTGGTTGA+GGG + chr8.3:24649365-24649384 MS.gene87328:CDS 50.0%
TGAGTCGTTAACTCAGCAGC+TGG - chr8.3:24649609-24649628 None:intergenic 50.0%
TGCCAACCCATCATCACCAT+CGG - chr8.3:24649679-24649698 None:intergenic 50.0%
TGGTGTATGAAGCTGATGCC+TGG + chr8.3:24649358-24649377 MS.gene87328:CDS 50.0%
! CCTCCACCGACTATCATAGT+CGG - chr8.3:24649564-24649583 None:intergenic 50.0%
!! ACAGAAGGAGCATCAACAGG+AGG - chr8.3:24649831-24649850 None:intergenic 50.0%
!! AGCACAGAAGGAGCATCAAC+AGG - chr8.3:24649834-24649853 None:intergenic 50.0%
!! CATCAACAGGAGGAAGCATC+TGG - chr8.3:24649821-24649840 None:intergenic 50.0%
!! GTTCATGATTCTGGGTTGGC+TGG + chr8.3:24649792-24649811 MS.gene87328:CDS 50.0%
!! TGTTCCTGTTGGTGGTGGTT+CGG + chr8.3:24649719-24649738 MS.gene87328:CDS 50.0%
AAACGCTCCGGGTTGTCCTT+TGG - chr8.3:24649246-24649265 None:intergenic 55.0%
AGCTTGGGTGGCACTTCCTA+TGG - chr8.3:24649203-24649222 None:intergenic 55.0%
ATGGATAGGGTCCCTCAACC+AGG - chr8.3:24649379-24649398 None:intergenic 55.0%
CAGGAACATCAGCACCCATC+TGG - chr8.3:24649773-24649792 None:intergenic 55.0%
CCAACCCATCATCACCATCG+GGG - chr8.3:24649677-24649696 None:intergenic 55.0%
CCGACTATGATAGTCGGTGG+AGG + chr8.3:24649561-24649580 MS.gene87328:CDS 55.0%
GAGCTAAGACACAGCTTGGG+TGG - chr8.3:24649215-24649234 None:intergenic 55.0%
GATAGTCGGTGGAGGACCAT+TGG + chr8.3:24649569-24649588 MS.gene87328:CDS 55.0%
GCCAACCCATCATCACCATC+GGG - chr8.3:24649678-24649697 None:intergenic 55.0%
GCGTTTCGCCAAGGTACACT+CGG + chr8.3:24649260-24649279 MS.gene87328:CDS 55.0%
GGCTCGGCAAGATTCCAGAT+GGG + chr8.3:24649756-24649775 MS.gene87328:CDS 55.0%
TGGTTGAGGGACCCTATCCA+TGG + chr8.3:24649378-24649397 MS.gene87328:CDS 55.0%
TGTACCTTGGCGAAACGCTC+CGG - chr8.3:24649258-24649277 None:intergenic 55.0%
! AATGGCCCCGATGGTGATGA+TGG + chr8.3:24649669-24649688 MS.gene87328:CDS 55.0%
! ATGGCCCCGATGGTGATGAT+GGG + chr8.3:24649670-24649689 MS.gene87328:CDS 55.0%
! CCACCGACTATCATAGTCGG+TGG - chr8.3:24649561-24649580 None:intergenic 55.0%
! CCACCGACTATGATAGTCGG+TGG + chr8.3:24649558-24649577 MS.gene87328:CDS 55.0%
! TGGCTCGGCAAGATTCCAGA+TGG + chr8.3:24649755-24649774 MS.gene87328:CDS 55.0%
!! GATGGTGATGATGGGTTGGC+AGG + chr8.3:24649678-24649697 MS.gene87328:CDS 55.0%
!! GCCAAGGTACACTCGGTGTT+TGG + chr8.3:24649267-24649286 MS.gene87328:CDS 55.0%
!! TGGGTTGGCAGGATTCCAGA+TGG + chr8.3:24649689-24649708 MS.gene87328:CDS 55.0%
GACCCGCACAGCCATGGATA+GGG - chr8.3:24649392-24649411 None:intergenic 60.0%
GACCCTATCCATGGCTGTGC+GGG + chr8.3:24649387-24649406 MS.gene87328:CDS 60.0%
GGACCCTATCCATGGCTGTG+CGG + chr8.3:24649386-24649405 MS.gene87328:CDS 60.0%
GTACCTTGGCGAAACGCTCC+GGG - chr8.3:24649257-24649276 None:intergenic 60.0%
!! CCCCGATGGTGATGATGGGT+TGG + chr8.3:24649674-24649693 MS.gene87328:CDS 60.0%
!! CCTGTTGGTGGTGGTTCGGT+CGG + chr8.3:24649723-24649742 MS.gene87328:CDS 60.0%
CCGACCGAACCACCACCAAC+AGG - chr8.3:24649726-24649745 None:intergenic 65.0%
GCAACCAGGGTCGATTGCCG+AGG + chr8.3:24649901-24649920 MS.gene87328:CDS 65.0%
GGACCCGCACAGCCATGGAT+AGG - chr8.3:24649393-24649412 None:intergenic 65.0%
TTGCTGGACCCGCACAGCCA+TGG - chr8.3:24649398-24649417 None:intergenic 65.0%
! CAACCCGGAGCGTTTCGCCA+AGG + chr8.3:24649251-24649270 MS.gene87328:CDS 65.0%
!! GTTGGTGGTGGTTCGGTCGG+TGG + chr8.3:24649726-24649745 MS.gene87328:CDS 65.0%
GGCTCCTCGGCAATCGACCC+TGG - chr8.3:24649908-24649927 None:intergenic 70.0%
GGTCGGTGGCAGTGGTGGCT+CGG + chr8.3:24649740-24649759 MS.gene87328:CDS 70.0%
! GGTTCGGTCGGTGGCAGTGG+TGG + chr8.3:24649735-24649754 MS.gene87328:CDS 70.0%
!! GGTGGTTCGGTCGGTGGCAG+TGG + chr8.3:24649732-24649751 MS.gene87328:CDS 70.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 24649156 24649938 24649156 ID=MS.gene87328
chr8.3 mRNA 24649156 24649938 24649156 ID=MS.gene87328.t1;Parent=MS.gene87328
chr8.3 exon 24649156 24649938 24649156 ID=MS.gene87328.t1.exon1;Parent=MS.gene87328.t1
chr8.3 CDS 24649156 24649938 24649156 ID=cds.MS.gene87328.t1;Parent=MS.gene87328.t1
Gene Sequence

>MS.gene87328

ATGGATAGAGAGATTATTCGCCCATGTGCAGCTTGCAAGCATCTCCATAGGAAGTGCCACCCAAGCTGTGTCTTAGCTCCTTACTTTCCAAAGGACAACCCGGAGCGTTTCGCCAAGGTACACTCGGTGTTTGGAAAGAGAAATGTGACTAATCTGTTGAAAAAACTGGATGTTTCACATCATGAACAAGCCGTTGAATCCTTGGTGTATGAAGCTGATGCCTGGTTGAGGGACCCTATCCATGGCTGTGCGGGTCCAGCAAATGACCTTCAAGGTAGGTTGAATGAAGCTCAAACGGAGCTCAAGAGTATGAAGAATGAACTGGCCAAATACCTCAATCCTCAGGTCGTTGAATTTGTTCTCGCCTTTCCTGATTGTTTCGGCTTTAAGCCAAATGCTTCTCCACCGACTATGATAGTCGGTGGAGGACCATTGGGTTTTAATCAGCTGCCAGCTGCTGAGTTAACGACTCAGCAGCTTCACAACCTAGAAGTTCTAAAACAAAAGCTTCTTAATGGCCCCGATGGTGATGATGGGTTGGCAGGATTCCAGATGGATGTTAATGTTCCTGTTGGTGGTGGTTCGGTCGGTGGCAGTGGTGGCTCGGCAAGATTCCAGATGGGTGCTGATGTTCCTGTTCATGATTCTGGGTTGGCTGGATCCCAGATGCTTCCTCCTGTTGATGCTCCTTCTGTGCTTCCTTCTTCTAGGGCTGATGCTTTTGACAATGGTAGCACTAGCACAAGCAACCAGGGTCGATTGCCGAGGAGCCACAACGTTTGA
Protein sequence

>MS.gene87328.t1

MDREIIRPCAACKHLHRKCHPSCVLAPYFPKDNPERFAKVHSVFGKRNVTNLLKKLDVSHHEQAVESLVYEADAWLRDPIHGCAGPANDLQGRLNEAQTELKSMKNELAKYLNPQVVEFVLAFPDCFGFKPNASPPTMIVGGGPLGFNQLPAAELTTQQLHNLEVLKQKLLNGPDGDDGLAGFQMDVNVPVGGGSVGGSGGSARFQMGADVPVHDSGLAGSQMLPPVDAPSVLPSSRADAFDNGSTSTSNQGRLPRSHNV