AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene87348

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene87348.t1 MTR_8g479350 89.459 351 28 4 1 350 1 343 0.0 562
MS.gene87348.t1 MTR_4g063780 68.354 79 16 1 70 139 72 150 3.61e-33 125
MS.gene87348.t1 MTR_2g096740 76.190 63 15 0 73 135 62 124 3.97e-33 124
MS.gene87348.t1 MTR_2g096740 76.190 63 15 0 73 135 77 139 4.12e-33 125
MS.gene87348.t1 MTR_7g024670 57.732 97 33 2 41 137 53 141 2.82e-32 124
MS.gene87348.t1 MTR_8g068210 55.556 90 40 0 60 149 19 108 3.33e-31 120
MS.gene87348.t1 MTR_5g031440 92.157 51 4 0 73 123 43 93 5.65e-31 120
MS.gene87348.t1 MTR_4g088580 79.661 59 12 0 74 132 56 114 7.73e-31 120
MS.gene87348.t1 MTR_7g059400 78.947 57 12 0 70 126 73 129 2.69e-30 118
MS.gene87348.t1 MTR_3g090430 65.333 75 22 1 56 126 30 104 3.48e-30 117
MS.gene87348.t1 MTR_8g079060 57.143 84 36 0 65 148 21 104 5.44e-30 114
MS.gene87348.t1 MTR_8g027295 63.158 76 28 0 70 145 21 96 5.63e-30 115
MS.gene87348.t1 MTR_3g091820 62.338 77 29 0 50 126 20 96 1.04e-29 116
MS.gene87348.t1 MTR_4g461080 84.906 53 8 0 73 125 29 81 1.64e-29 114
MS.gene87348.t1 MTR_4g022370 81.481 54 10 0 73 126 82 135 1.79e-29 116
MS.gene87348.t1 MTR_4g109980 86.275 51 7 0 73 123 40 90 2.67e-29 115
MS.gene87348.t1 MTR_8g015840 80.000 55 11 0 70 124 17 71 3.53e-29 112
MS.gene87348.t1 MTR_2g093220 85.185 54 8 0 73 126 40 93 5.00e-29 114
MS.gene87348.t1 MTR_1g056810 81.034 58 11 0 69 126 8 65 1.84e-28 112
MS.gene87348.t1 MTR_2g014060 83.636 55 8 1 70 124 23 76 2.86e-28 112
MS.gene87348.t1 MTR_3g077750 53.608 97 35 3 33 123 19 111 7.81e-28 111
MS.gene87348.t1 MTR_4g089095 78.947 57 11 1 72 128 49 104 1.04e-27 110
MS.gene87348.t1 MTR_4g089095 78.947 57 11 1 72 128 15 70 1.05e-27 109
MS.gene87348.t1 MTR_2g059540 85.714 49 7 0 73 121 31 79 2.25e-27 107
MS.gene87348.t1 MTR_1g077600 72.414 58 16 0 68 125 38 95 7.81e-27 107
MS.gene87348.t1 MTR_1g077600 72.414 58 16 0 68 125 38 95 8.17e-27 107
MS.gene87348.t1 MTR_2g014170 83.673 49 8 0 73 121 45 93 8.54e-27 108
MS.gene87348.t1 MTR_2g016030 45.455 110 54 2 62 171 36 139 1.25e-26 103
MS.gene87348.t1 MTR_7g010950 53.333 90 40 1 40 127 100 189 6.20e-26 108
MS.gene87348.t1 MTR_2g013370 73.214 56 14 1 73 128 46 100 1.20e-25 104
MS.gene87348.t1 MTR_6g012450 75.926 54 13 0 73 126 136 189 3.26e-25 106
MS.gene87348.t1 MTR_7g086780 57.895 76 32 0 73 148 99 174 7.35e-25 104
MS.gene87348.t1 MTR_6g027460 35.912 181 93 3 42 204 57 232 7.77e-25 103
MS.gene87348.t1 MTR_5g041420 52.577 97 40 2 44 135 105 200 8.40e-25 103
MS.gene87348.t1 MTR_4g082060 51.064 94 40 2 44 131 87 180 1.34e-24 104
MS.gene87348.t1 MTR_3g435480 72.222 54 15 0 73 126 136 189 1.55e-24 104
MS.gene87348.t1 MTR_6g027450 48.421 95 41 1 42 128 50 144 1.71e-24 102
MS.gene87348.t1 MTR_8g044220 56.164 73 32 0 73 145 118 190 1.96e-24 103
MS.gene87348.t1 MTR_5g041530 45.872 109 53 2 35 138 97 204 1.09e-23 100
MS.gene87348.t1 MTR_5g041380 47.872 94 47 1 44 135 96 189 1.16e-22 97.8
MS.gene87348.t1 MTR_5g041400 48.936 94 46 1 44 135 88 181 1.28e-22 97.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene87348.t1 AT5G60200 58.416 101 35 3 63 160 42 138 2.64e-33 124
MS.gene87348.t1 AT1G07640 90.566 53 5 0 73 125 21 73 4.68e-32 121
MS.gene87348.t1 AT5G65590 53.398 103 43 1 35 137 8 105 6.95e-32 122
MS.gene87348.t1 AT1G07640 90.566 53 5 0 73 125 77 129 7.36e-32 122
MS.gene87348.t1 AT1G07640 90.566 53 5 0 73 125 85 137 9.68e-32 122
MS.gene87348.t1 AT1G51700 73.134 67 12 1 64 124 18 84 1.77e-31 117
MS.gene87348.t1 AT2G28810 86.538 52 7 0 73 124 77 128 2.83e-31 120
MS.gene87348.t1 AT2G28810 86.538 52 7 0 73 124 94 145 4.22e-31 120
MS.gene87348.t1 AT5G60850 72.222 72 15 1 73 139 51 122 4.60e-31 119
MS.gene87348.t1 AT3G21270 88.679 53 6 0 73 125 29 81 8.98e-31 116
MS.gene87348.t1 AT2G37590 78.947 57 12 0 70 126 86 142 9.29e-31 119
MS.gene87348.t1 AT4G38000 68.421 76 23 1 52 126 20 95 2.88e-30 116
MS.gene87348.t1 AT3G45610 69.697 66 20 0 73 138 40 105 6.57e-30 115
MS.gene87348.t1 AT5G02460 83.333 54 9 0 73 126 95 148 1.06e-29 117
MS.gene87348.t1 AT3G55370 73.134 67 15 1 73 139 76 139 1.65e-29 115
MS.gene87348.t1 AT3G55370 73.134 67 15 1 73 139 76 139 1.71e-29 116
MS.gene87348.t1 AT3G55370 70.000 70 18 1 70 139 118 184 2.25e-29 116
MS.gene87348.t1 AT3G52440 73.846 65 15 1 62 126 16 78 1.00e-28 112
MS.gene87348.t1 AT3G50410 73.016 63 17 0 63 125 20 82 1.10e-28 112
MS.gene87348.t1 AT3G52440 73.846 65 15 1 62 126 35 97 1.18e-28 112
MS.gene87348.t1 AT1G28310 78.571 56 12 0 73 128 27 82 1.33e-28 113
MS.gene87348.t1 AT3G61850 55.319 94 41 1 34 126 22 115 1.48e-28 112
MS.gene87348.t1 AT3G61850 55.319 94 41 1 34 126 22 115 1.48e-28 112
MS.gene87348.t1 AT3G61850 55.319 94 41 1 34 126 34 127 1.88e-28 112
MS.gene87348.t1 AT1G28310 78.571 56 12 0 73 128 51 106 2.00e-28 113
MS.gene87348.t1 AT3G61850 55.319 94 41 1 34 126 34 127 2.31e-28 112
MS.gene87348.t1 AT3G61850 55.319 94 41 1 34 126 22 115 2.40e-28 112
MS.gene87348.t1 AT2G28510 82.692 52 9 0 75 126 50 101 2.52e-28 112
MS.gene87348.t1 AT5G66940 60.274 73 29 0 67 139 26 98 3.79e-28 109
MS.gene87348.t1 AT1G21340 55.056 89 39 1 75 162 40 128 1.11e-27 109
MS.gene87348.t1 AT4G00940 57.471 87 37 0 63 149 58 144 1.33e-27 110
MS.gene87348.t1 AT4G00940 57.471 87 37 0 63 149 58 144 1.33e-27 110
MS.gene87348.t1 AT4G00940 57.471 87 37 0 63 149 58 144 1.33e-27 110
MS.gene87348.t1 AT4G21050 56.790 81 27 1 62 134 5 85 2.62e-27 107
MS.gene87348.t1 AT4G24060 70.968 62 18 0 65 126 45 106 8.32e-27 108
MS.gene87348.t1 AT5G62940 80.000 50 10 0 74 123 74 123 3.28e-26 107
MS.gene87348.t1 AT1G29160 58.667 75 31 0 52 126 41 115 1.06e-25 102
MS.gene87348.t1 AT2G46590 54.082 98 33 4 34 120 19 115 2.99e-25 105
MS.gene87348.t1 AT2G34140 55.263 76 34 0 51 126 36 111 3.69e-25 100
MS.gene87348.t1 AT2G46590 54.082 98 33 4 34 120 31 127 3.69e-25 105
MS.gene87348.t1 AT3G47500 74.545 55 14 0 73 127 110 164 6.63e-25 105
MS.gene87348.t1 AT5G39660 50.467 107 36 1 38 127 86 192 7.48e-25 105
MS.gene87348.t1 AT5G39660 50.467 107 36 1 38 127 86 192 7.48e-25 105
MS.gene87348.t1 AT5G39660 50.467 107 36 1 38 127 86 192 7.48e-25 105
MS.gene87348.t1 AT1G64620 51.724 87 41 1 36 121 11 97 1.32e-24 103
MS.gene87348.t1 AT1G69570 55.844 77 34 0 63 139 122 198 1.47e-24 103
MS.gene87348.t1 AT1G26790 58.904 73 27 2 57 126 92 164 2.81e-24 102
MS.gene87348.t1 AT1G47655 50.588 85 32 2 37 121 3 77 3.05e-24 99.0
MS.gene87348.t1 AT5G62430 74.074 54 14 0 73 126 54 107 3.92e-24 100
MS.gene87348.t1 AT4G21040 73.585 53 14 0 75 127 27 79 4.15e-23 96.7
MS.gene87348.t1 AT4G21080 61.538 65 25 0 75 139 27 91 2.08e-22 95.1
MS.gene87348.t1 AT4G21030 53.704 54 25 0 71 124 19 72 1.63e-13 68.9

Find 101 sgRNAs with CRISPR-Local

Find 108 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCATGATTGTTTCTCCAAAA+TGG 0.139233 8.3:+25073931 None:intergenic
TTCTTCTATATCCAACATTT+TGG 0.168024 8.3:-25073713 MS.gene87348:CDS
TCCTCCAATTCCTCCATTAA+TGG 0.206223 8.3:+25074566 None:intergenic
GCAGTTACTGGTGGTGAATT+AGG 0.209085 8.3:-25073682 MS.gene87348:CDS
GTTATACTTATTACTGATAT+TGG 0.212000 8.3:+25073593 None:intergenic
CATGATTAGGGTTAGGGTTT+TGG 0.228883 8.3:+25074064 None:intergenic
CCTTCTTCCTTAACATGATT+AGG 0.237816 8.3:+25074051 None:intergenic
CACCATTACCAAAGAGTTCT+TGG 0.238413 8.3:+25074628 None:intergenic
GTGGTGGTGGTTGTGGAAGA+TGG 0.243950 8.3:+25074504 None:intergenic
TTAGTTTCTGGTACAGTTCT+TGG 0.271995 8.3:+25073830 None:intergenic
CTTCTTCCTTAACATGATTA+GGG 0.273935 8.3:+25074052 None:intergenic
AGAAACTACTCTTCCATTAA+TGG 0.274062 8.3:-25074579 MS.gene87348:CDS
ACAGTTTCTTCAGAGTTTAA+TGG 0.275960 8.3:-25074204 MS.gene87348:CDS
ATCTTAGCAAAGAAAGTATA+TGG 0.279631 8.3:+25074097 None:intergenic
GCAAGACATGCCGTCGTTAC+TGG 0.280270 8.3:-25074335 MS.gene87348:CDS
TTGGAAGATCAGACCAAGTT+AGG 0.282710 8.3:+25073638 None:intergenic
TTGGAGACAACTGCAGTTAC+TGG 0.305247 8.3:-25073694 MS.gene87348:CDS
TTGTTGATGACCTTAGTTTC+TGG 0.307492 8.3:+25073818 None:intergenic
GAAGAAGGCAATAACTTCAT+AGG 0.324773 8.3:-25074036 MS.gene87348:CDS
TGGTGCAGTGGTGGTGGTGG+TGG 0.332078 8.3:+25074488 None:intergenic
TTATACTTATTACTGATATT+GGG 0.334867 8.3:+25073594 None:intergenic
TCTTCCATTAATGGAGGAAT+TGG 0.337768 8.3:-25074570 MS.gene87348:CDS
CAAAATGGTGGATTTGTACT+TGG 0.338045 8.3:-25073889 MS.gene87348:CDS
TCTTCACCAACAGTGTTACT+AGG 0.338562 8.3:-25073754 MS.gene87348:CDS
CCTTAACATGATTAGGGTTA+GGG 0.353211 8.3:+25074058 None:intergenic
CAAACTCAACAATTACAAAA+TGG 0.354663 8.3:-25073904 MS.gene87348:CDS
GACCAAGTTAGGGTTGGAAT+TGG 0.358551 8.3:+25073649 None:intergenic
TCCTTAACATGATTAGGGTT+AGG 0.359817 8.3:+25074057 None:intergenic
CATGATTGTTTCTCCAAAAT+GGG 0.372690 8.3:+25073932 None:intergenic
CAGAAAACCAACCACATCTA+AGG 0.374661 8.3:-25074434 MS.gene87348:CDS
TGGTGGTGGTTGTGGAAGAT+GGG 0.383636 8.3:+25074505 None:intergenic
TTTCCGACAGCCACCTCCTA+TGG 0.383757 8.3:+25074284 None:intergenic
CAGTTACTGGTGGTGAATTA+GGG 0.399545 8.3:-25073681 MS.gene87348:CDS
GACGGCATGTCTTGCAGAAA+TGG 0.400957 8.3:+25074343 None:intergenic
CACCACAAAGTCAAATCCTC+TGG 0.406936 8.3:-25074137 MS.gene87348:CDS
CAACCGTTGATTTCGTCAAA+TGG 0.418354 8.3:-25073985 MS.gene87348:CDS
GAAGAAGAAGGTTGTGTTGT+TGG 0.421244 8.3:+25074531 None:intergenic
CCCTAACCCTAATCATGTTA+AGG 0.421766 8.3:-25074058 MS.gene87348:CDS
CCCCAGAGGATTTGACTTTG+TGG 0.425921 8.3:+25074135 None:intergenic
CCTAATCATGTTAAGGAAGA+AGG 0.447706 8.3:-25074051 MS.gene87348:CDS
TCCATTAATGGAGGAATTGG+AGG 0.466200 8.3:-25074567 MS.gene87348:CDS
GGACAAAAGGAGGTGCTTTG+AGG 0.473653 8.3:-25074314 MS.gene87348:CDS
ATCCAATTCCAACCCTAACT+TGG 0.473948 8.3:-25073651 MS.gene87348:CDS
GGTGGTTGGTGCAGTGGTGG+TGG 0.475125 8.3:+25074482 None:intergenic
TGATGATGCGGTGGTGTTTG+TGG 0.476948 8.3:+25074455 None:intergenic
AAGATGATCCAAGAACTCTT+TGG 0.477513 8.3:-25074636 MS.gene87348:CDS
TGCCGTCGTTACTGGACAAA+AGG 0.477938 8.3:-25074327 MS.gene87348:CDS
AAAATGGTGGATTTGTACTT+GGG 0.478306 8.3:-25073888 MS.gene87348:CDS
ATTTCGTCAAATGGTTATGA+TGG 0.478918 8.3:-25073976 MS.gene87348:CDS
GGTTGGTGCAGTGGTGGTGG+TGG 0.494544 8.3:+25074485 None:intergenic
TGCGGTGGTGTTTGTGGTGA+CGG 0.495255 8.3:+25074461 None:intergenic
AAGAACTGTACCAGAAACTA+AGG 0.497513 8.3:-25073828 MS.gene87348:CDS
TGGAAGATCAGACCAAGTTA+GGG 0.500768 8.3:+25073639 None:intergenic
CTGGTGGTGAATTAGGGTAC+TGG 0.503929 8.3:-25073675 MS.gene87348:CDS
TTGGGTTTGAATTGCACCAT+TGG 0.506743 8.3:+25073612 None:intergenic
GTGTTTGTGGTGACGGTGGT+TGG 0.508002 8.3:+25074468 None:intergenic
TCAACAACATCATCATCATC+AGG 0.508236 8.3:-25073802 MS.gene87348:CDS
GTGTTTGATGAGTCACATCT+TGG 0.511366 8.3:+25074408 None:intergenic
TGCAGTTGTCTCCAAAATGT+TGG 0.512043 8.3:+25073702 None:intergenic
ATCCAAGAACTCTTTGGTAA+TGG 0.516650 8.3:-25074630 MS.gene87348:CDS
TTGGACACCTTAGATGTGGT+TGG 0.518935 8.3:+25074427 None:intergenic
GACGGTGGTTGGTGCAGTGG+TGG 0.519460 8.3:+25074479 None:intergenic
TGAATTGCACCATTGGTAGT+TGG 0.526899 8.3:+25073619 None:intergenic
ACCACAAAGTCAAATCCTCT+GGG 0.532491 8.3:-25074136 MS.gene87348:CDS
AAGAAGGCAATAACTTCATA+GGG 0.534937 8.3:-25074035 MS.gene87348:CDS
TTCCGACAGCCACCTCCTAT+GGG 0.538444 8.3:+25074285 None:intergenic
GGAAGGTCAACAAGTGGTCA+AGG 0.546021 8.3:-25074183 MS.gene87348:CDS
TCTTAGCAAAGAAAGTATAT+GGG 0.546548 8.3:+25074098 None:intergenic
GTTTCTTTCACTTGAAGTGA+GGG 0.555832 8.3:+25074596 None:intergenic
GGTGACGGTGGTTGGTGCAG+TGG 0.560684 8.3:+25074476 None:intergenic
TTGAGGAACGTTCCCATAGG+AGG 0.560915 8.3:-25074297 MS.gene87348:CDS
GAAGATGGGCGTGAAGAAGA+AGG 0.562429 8.3:+25074519 None:intergenic
CTTCACCAACAGTGTTACTA+GGG 0.563883 8.3:-25073753 MS.gene87348:CDS
CAGAGGATTTGACTTTGTGG+TGG 0.563970 8.3:+25074138 None:intergenic
AGGAACGTTCCCATAGGAGG+TGG 0.565496 8.3:-25074294 MS.gene87348:CDS
AGATCAGACCAAGTTAGGGT+TGG 0.572219 8.3:+25073643 None:intergenic
TCAAATGGTTATGATGGTGT+TGG 0.575378 8.3:-25073970 MS.gene87348:CDS
AGTTTCTTTCACTTGAAGTG+AGG 0.580149 8.3:+25074595 None:intergenic
AAATGGTGGATTTGTACTTG+GGG 0.583095 8.3:-25073887 MS.gene87348:CDS
TTCCCATAGGAGGTGGCTGT+CGG 0.589355 8.3:-25074287 MS.gene87348:CDS
ACTCAACAATTACAAAATGG+TGG 0.590751 8.3:-25073901 MS.gene87348:CDS
TCTGATCTTCCAACTACCAA+TGG 0.595789 8.3:-25073628 MS.gene87348:CDS
TTTCTTCAGAGTTTAATGGA+AGG 0.603721 8.3:-25074200 MS.gene87348:CDS
AATTCATCATGTGAGAAACA+TGG 0.605341 8.3:+25074010 None:intergenic
ATTCATCATGTGAGAAACAT+GGG 0.610019 8.3:+25074011 None:intergenic
GAAGGTCAACAAGTGGTCAA+GGG 0.612566 8.3:-25074182 MS.gene87348:CDS
TAACCATTTGACGAAATCAA+CGG 0.616215 8.3:+25073982 None:intergenic
CGTCGTTACTGGACAAAAGG+AGG 0.618133 8.3:-25074324 MS.gene87348:CDS
GCTTTGAGGAACGTTCCCAT+AGG 0.619952 8.3:-25074300 MS.gene87348:CDS
AACTACTCTTCCATTAATGG+AGG 0.623980 8.3:-25074576 MS.gene87348:CDS
CATCTTGGACACCTTAGATG+TGG 0.628308 8.3:+25074423 None:intergenic
CATGTCTTGCAGAAATGGCG+AGG 0.648175 8.3:+25074348 None:intergenic
TTTAATGGAAGGTCAACAAG+TGG 0.666542 8.3:-25074189 MS.gene87348:CDS
ACGTTCCCTAGTAACACTGT+TGG 0.681503 8.3:+25073748 None:intergenic
AACAGTGTTACTAGGGAACG+TGG 0.681785 8.3:-25073746 MS.gene87348:CDS
CATCACCATCATCAAAACAG+TGG 0.687081 8.3:-25073856 MS.gene87348:CDS
AGAAATGGCGAGGCTGAGTG+AGG 0.701300 8.3:+25074358 None:intergenic
GGTGGTGTTTGTGGTGACGG+TGG 0.703645 8.3:+25074464 None:intergenic
TATCAACGCCTCAAATAGCG+CGG 0.706731 8.3:-25074253 MS.gene87348:CDS
GAGACAACTGCAGTTACTGG+TGG 0.722534 8.3:-25073691 MS.gene87348:CDS
CCACAAAGTCAAATCCTCTG+GGG 0.739195 8.3:-25074135 MS.gene87348:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! CAAACTCAACAATTACAAAA+TGG - chr8.3:25074332-25074351 MS.gene87348:CDS 25.0%
!! ATCTTAGCAAAGAAAGTATA+TGG + chr8.3:25074142-25074161 None:intergenic 25.0%
!! TCTTAGCAAAGAAAGTATAT+GGG + chr8.3:25074141-25074160 None:intergenic 25.0%
!! TTCTTCTATATCCAACATTT+TGG - chr8.3:25074523-25074542 MS.gene87348:CDS 25.0%
AAGAAGGCAATAACTTCATA+GGG - chr8.3:25074201-25074220 MS.gene87348:CDS 30.0%
AATTCATCATGTGAGAAACA+TGG + chr8.3:25074229-25074248 None:intergenic 30.0%
ACAGTTTCTTCAGAGTTTAA+TGG - chr8.3:25074032-25074051 MS.gene87348:CDS 30.0%
ACTCAACAATTACAAAATGG+TGG - chr8.3:25074335-25074354 MS.gene87348:CDS 30.0%
AGAAACTACTCTTCCATTAA+TGG - chr8.3:25073657-25073676 MS.gene87348:CDS 30.0%
ATTCATCATGTGAGAAACAT+GGG + chr8.3:25074228-25074247 None:intergenic 30.0%
ATTTCGTCAAATGGTTATGA+TGG - chr8.3:25074260-25074279 MS.gene87348:CDS 30.0%
CTTCTTCCTTAACATGATTA+GGG + chr8.3:25074187-25074206 None:intergenic 30.0%
TAACCATTTGACGAAATCAA+CGG + chr8.3:25074257-25074276 None:intergenic 30.0%
TTTCTTCAGAGTTTAATGGA+AGG - chr8.3:25074036-25074055 MS.gene87348:CDS 30.0%
! ATGTTTTATGCAACCCATTT+TGG - chr8.3:25074291-25074310 MS.gene87348:CDS 30.0%
! CATGATTGTTTCTCCAAAAT+GGG + chr8.3:25074307-25074326 None:intergenic 30.0%
! TCATGATTGTTTCTCCAAAA+TGG + chr8.3:25074308-25074327 None:intergenic 30.0%
!! AAAATGGTGGATTTGTACTT+GGG - chr8.3:25074348-25074367 MS.gene87348:CDS 30.0%
AACTACTCTTCCATTAATGG+AGG - chr8.3:25073660-25073679 MS.gene87348:CDS 35.0%
AAGAACTGTACCAGAAACTA+AGG - chr8.3:25074408-25074427 MS.gene87348:CDS 35.0%
AGTTTCTTTCACTTGAAGTG+AGG + chr8.3:25073644-25073663 None:intergenic 35.0%
ATCCAAGAACTCTTTGGTAA+TGG - chr8.3:25073606-25073625 MS.gene87348:CDS 35.0%
CCTAATCATGTTAAGGAAGA+AGG - chr8.3:25074185-25074204 MS.gene87348:CDS 35.0%
CCTTAACATGATTAGGGTTA+GGG + chr8.3:25074181-25074200 None:intergenic 35.0%
CCTTCTTCCTTAACATGATT+AGG + chr8.3:25074188-25074207 None:intergenic 35.0%
GAAGAAGGCAATAACTTCAT+AGG - chr8.3:25074200-25074219 MS.gene87348:CDS 35.0%
GTTTCTTTCACTTGAAGTGA+GGG + chr8.3:25073643-25073662 None:intergenic 35.0%
TCAACAACATCATCATCATC+AGG - chr8.3:25074434-25074453 MS.gene87348:CDS 35.0%
TCCTTAACATGATTAGGGTT+AGG + chr8.3:25074182-25074201 None:intergenic 35.0%
TCTTCCATTAATGGAGGAAT+TGG - chr8.3:25073666-25073685 MS.gene87348:CDS 35.0%
TTTAATGGAAGGTCAACAAG+TGG - chr8.3:25074047-25074066 MS.gene87348:CDS 35.0%
! TCAAATGGTTATGATGGTGT+TGG - chr8.3:25074266-25074285 MS.gene87348:CDS 35.0%
!! AAATGGTGGATTTGTACTTG+GGG - chr8.3:25074349-25074368 MS.gene87348:CDS 35.0%
!! CAAAATGGTGGATTTGTACT+TGG - chr8.3:25074347-25074366 MS.gene87348:CDS 35.0%
!! GAAAGTATATGGGAGTTTTG+AGG + chr8.3:25074131-25074150 None:intergenic 35.0%
!! TTAGTTTCTGGTACAGTTCT+TGG + chr8.3:25074409-25074428 None:intergenic 35.0%
!! TTGTTGATGACCTTAGTTTC+TGG + chr8.3:25074421-25074440 None:intergenic 35.0%
ACCACAAAGTCAAATCCTCT+GGG - chr8.3:25074100-25074119 MS.gene87348:CDS 40.0%
ATCCAATTCCAACCCTAACT+TGG - chr8.3:25074585-25074604 MS.gene87348:CDS 40.0%
CACCATTACCAAAGAGTTCT+TGG + chr8.3:25073611-25073630 None:intergenic 40.0%
CAGAAAACCAACCACATCTA+AGG - chr8.3:25073802-25073821 MS.gene87348:CDS 40.0%
CATCACCATCATCAAAACAG+TGG - chr8.3:25074380-25074399 MS.gene87348:CDS 40.0%
CCCTAACCCTAATCATGTTA+AGG - chr8.3:25074178-25074197 MS.gene87348:CDS 40.0%
GAAGAAGAAGGTTGTGTTGT+TGG + chr8.3:25073708-25073727 None:intergenic 40.0%
GTGTTTGATGAGTCACATCT+TGG + chr8.3:25073831-25073850 None:intergenic 40.0%
TCCATTAATGGAGGAATTGG+AGG - chr8.3:25073669-25073688 MS.gene87348:CDS 40.0%
TCCTCCAATTCCTCCATTAA+TGG + chr8.3:25073673-25073692 None:intergenic 40.0%
TCTGATCTTCCAACTACCAA+TGG - chr8.3:25074608-25074627 MS.gene87348:CDS 40.0%
TGCAGTTGTCTCCAAAATGT+TGG + chr8.3:25074537-25074556 None:intergenic 40.0%
TGGAAGATCAGACCAAGTTA+GGG + chr8.3:25074600-25074619 None:intergenic 40.0%
TTGGAAGATCAGACCAAGTT+AGG + chr8.3:25074601-25074620 None:intergenic 40.0%
TTGGGTTTGAATTGCACCAT+TGG + chr8.3:25074627-25074646 None:intergenic 40.0%
! CAGTTACTGGTGGTGAATTA+GGG - chr8.3:25074555-25074574 MS.gene87348:CDS 40.0%
! CATGATTAGGGTTAGGGTTT+TGG + chr8.3:25074175-25074194 None:intergenic 40.0%
! CTTCACCAACAGTGTTACTA+GGG - chr8.3:25074483-25074502 MS.gene87348:CDS 40.0%
! TCTTCACCAACAGTGTTACT+AGG - chr8.3:25074482-25074501 MS.gene87348:CDS 40.0%
! TGAATTGCACCATTGGTAGT+TGG + chr8.3:25074620-25074639 None:intergenic 40.0%
!! CAACCGTTGATTTCGTCAAA+TGG - chr8.3:25074251-25074270 MS.gene87348:CDS 40.0%
!!! TGGTTGGTTTTCTGATGATG+CGG + chr8.3:25073796-25073815 None:intergenic 40.0%
ACGTTCCCTAGTAACACTGT+TGG + chr8.3:25074491-25074510 None:intergenic 45.0%
AGATCAGACCAAGTTAGGGT+TGG + chr8.3:25074596-25074615 None:intergenic 45.0%
CACCACAAAGTCAAATCCTC+TGG - chr8.3:25074099-25074118 MS.gene87348:CDS 45.0%
CATCTTGGACACCTTAGATG+TGG + chr8.3:25073816-25073835 None:intergenic 45.0%
CCACAAAGTCAAATCCTCTG+GGG - chr8.3:25074101-25074120 MS.gene87348:CDS 45.0%
GAAGGTCAACAAGTGGTCAA+GGG - chr8.3:25074054-25074073 MS.gene87348:CDS 45.0%
GACCAAGTTAGGGTTGGAAT+TGG + chr8.3:25074590-25074609 None:intergenic 45.0%
TATCAACGCCTCAAATAGCG+CGG - chr8.3:25073983-25074002 MS.gene87348:CDS 45.0%
TTGGACACCTTAGATGTGGT+TGG + chr8.3:25073812-25073831 None:intergenic 45.0%
TTGGAGACAACTGCAGTTAC+TGG - chr8.3:25074542-25074561 MS.gene87348:CDS 45.0%
! AACAGTGTTACTAGGGAACG+TGG - chr8.3:25074490-25074509 MS.gene87348:CDS 45.0%
! CAGAGGATTTGACTTTGTGG+TGG + chr8.3:25074101-25074120 None:intergenic 45.0%
! CTCCTTTTGTCCAGTAACGA+CGG + chr8.3:25073914-25073933 None:intergenic 45.0%
! GCAGTTACTGGTGGTGAATT+AGG - chr8.3:25074554-25074573 MS.gene87348:CDS 45.0%
!! TGTTTTTGCCGCGCTATTTG+AGG + chr8.3:25073994-25074013 None:intergenic 45.0%
!! TTGGTTTTCTGATGATGCGG+TGG + chr8.3:25073793-25073812 None:intergenic 45.0%
!!! GGACTCCACTGTTTTGATGA+TGG + chr8.3:25074388-25074407 None:intergenic 45.0%
CATGTCTTGCAGAAATGGCG+AGG + chr8.3:25073891-25073910 None:intergenic 50.0%
CGTCGTTACTGGACAAAAGG+AGG - chr8.3:25073912-25073931 MS.gene87348:CDS 50.0%
GAAGATGGGCGTGAAGAAGA+AGG + chr8.3:25073720-25073739 None:intergenic 50.0%
GACGGCATGTCTTGCAGAAA+TGG + chr8.3:25073896-25073915 None:intergenic 50.0%
GAGACAACTGCAGTTACTGG+TGG - chr8.3:25074545-25074564 MS.gene87348:CDS 50.0%
GGAAGGTCAACAAGTGGTCA+AGG - chr8.3:25074053-25074072 MS.gene87348:CDS 50.0%
TGCCGTCGTTACTGGACAAA+AGG - chr8.3:25073909-25073928 MS.gene87348:CDS 50.0%
TTGAGGAACGTTCCCATAGG+AGG - chr8.3:25073939-25073958 MS.gene87348:CDS 50.0%
! CCCCAGAGGATTTGACTTTG+TGG + chr8.3:25074104-25074123 None:intergenic 50.0%
! CTGGTGGTGAATTAGGGTAC+TGG - chr8.3:25074561-25074580 MS.gene87348:CDS 50.0%
! TGATGATGCGGTGGTGTTTG+TGG + chr8.3:25073784-25073803 None:intergenic 50.0%
!! GCTTTGAGGAACGTTCCCAT+AGG - chr8.3:25073936-25073955 MS.gene87348:CDS 50.0%
!! GGACAAAAGGAGGTGCTTTG+AGG - chr8.3:25073922-25073941 MS.gene87348:CDS 50.0%
!! TGGTGGTGGTTGTGGAAGAT+GGG + chr8.3:25073734-25073753 None:intergenic 50.0%
!!! AGTTTTGAGGAGAACCCCAG+AGG + chr8.3:25074118-25074137 None:intergenic 50.0%
AGAAATGGCGAGGCTGAGTG+AGG + chr8.3:25073881-25073900 None:intergenic 55.0%
AGGAACGTTCCCATAGGAGG+TGG - chr8.3:25073942-25073961 MS.gene87348:CDS 55.0%
GCAAGACATGCCGTCGTTAC+TGG - chr8.3:25073901-25073920 MS.gene87348:CDS 55.0%
TGCGGTGGTGTTTGTGGTGA+CGG + chr8.3:25073778-25073797 None:intergenic 55.0%
TTCCCATAGGAGGTGGCTGT+CGG - chr8.3:25073949-25073968 MS.gene87348:CDS 55.0%
TTCCGACAGCCACCTCCTAT+GGG + chr8.3:25073954-25073973 None:intergenic 55.0%
TTTCCGACAGCCACCTCCTA+TGG + chr8.3:25073955-25073974 None:intergenic 55.0%
!! GTGGTGGTGGTTGTGGAAGA+TGG + chr8.3:25073735-25073754 None:intergenic 55.0%
!! GTGTTTGTGGTGACGGTGGT+TGG + chr8.3:25073771-25073790 None:intergenic 55.0%
!! GGTGGTGTTTGTGGTGACGG+TGG + chr8.3:25073775-25073794 None:intergenic 60.0%
! GACGGTGGTTGGTGCAGTGG+TGG + chr8.3:25073760-25073779 None:intergenic 65.0%
! GGTGACGGTGGTTGGTGCAG+TGG + chr8.3:25073763-25073782 None:intergenic 65.0%
! GGTGGTTGGTGCAGTGGTGG+TGG + chr8.3:25073757-25073776 None:intergenic 65.0%
!! GGTGGTGGTGGTGGTGGTTG+TGG + chr8.3:25073742-25073761 None:intergenic 65.0%
!! GGTTGGTGCAGTGGTGGTGG+TGG + chr8.3:25073754-25073773 None:intergenic 65.0%
!! TGCAGTGGTGGTGGTGGTGG+TGG + chr8.3:25073748-25073767 None:intergenic 65.0%
!! TGGTGCAGTGGTGGTGGTGG+TGG + chr8.3:25073751-25073770 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 25073603 25074655 25073603 ID=MS.gene87348
chr8.3 mRNA 25073603 25074655 25073603 ID=MS.gene87348.t1;Parent=MS.gene87348
chr8.3 exon 25073603 25074655 25073603 ID=MS.gene87348.t1.exon1;Parent=MS.gene87348.t1
chr8.3 CDS 25073603 25074655 25073603 ID=cds.MS.gene87348.t1;Parent=MS.gene87348.t1
Gene Sequence

>MS.gene87348

ATGATCCAAGAACTCTTTGGTAATGGTGCTAGTGCTACCCTCACTTCAAGTGAAAGAAACTACTCTTCCATTAATGGAGGAATTGGAGGAGTTTTACTTCAACCAACAACACAACCTTCTTCTTCACGCCCATCTTCCACAACCACCACCACCACCACCACTGCACCAACCACCGTCACCACAAACACCACCGCATCATCAGAAAACCAACCACATCTAAGGTGTCCAAGATGTGACTCATCAAACACAAAATTTTGTTACTACAACAACTACAACCTCACTCAGCCTCGCCATTTCTGCAAGACATGCCGTCGTTACTGGACAAAAGGAGGTGCTTTGAGGAACGTTCCCATAGGAGGTGGCTGTCGGAAAAACAAGAGTATCAACGCCTCAAATAGCGCGGCAAAAACAACTACAAACAAGATGAAAACAGTTTCTTCAGAGTTTAATGGAAGGTCAACAAGTGGTCAAGGGTTTGATCTCGAACACGTTGTTCCACCACAAAGTCAAATCCTCTGGGGTTCTCCTCAAAACTCCCATATACTTTCTTTGCTAAGATCTAGTCAAAACCAAAACCCTAACCCTAATCATGTTAAGGAAGAAGGCAATAACTTCATAGGGTCCCATGTTTCTCACATGATGAATTCTCAACCGTTGATTTCGTCAAATGGTTATGATGGTGTTGGACATGTTTTATGCAACCCATTTTGGAGAAACAATCATGATAATCAAACTCAACAATTACAAAATGGTGGATTTGTACTTGGGGATCAACAACATCACCATCATCAAAACAGTGGAGTCCAAGAACTGTACCAGAAACTAAGGTCATCAACAACATCATCATCATCAGGTAATAATTATCGCAGTGAGATTTCATCTTCACCAACAGTGTTACTAGGGAACGTGGCTTCAAATTCTTCTTCTATATCCAACATTTTGGAGACAACTGCAGTTACTGGTGGTGAATTAGGGTACTGGAATCCAATTCCAACCCTAACTTGGTCTGATCTTCCAACTACCAATGGTGCAATTCAAACCCAATATCAGTAA
Protein sequence

>MS.gene87348.t1

MIQELFGNGASATLTSSERNYSSINGGIGGVLLQPTTQPSSSRPSSTTTTTTTTAPTTVTTNTTASSENQPHLRCPRCDSSNTKFCYYNNYNLTQPRHFCKTCRRYWTKGGALRNVPIGGGCRKNKSINASNSAAKTTTNKMKTVSSEFNGRSTSGQGFDLEHVVPPQSQILWGSPQNSHILSLLRSSQNQNPNPNHVKEEGNNFIGSHVSHMMNSQPLISSNGYDGVGHVLCNPFWRNNHDNQTQQLQNGGFVLGDQQHHHHQNSGVQELYQKLRSSTTSSSSGNNYRSEISSSPTVLLGNVASNSSSISNILETTAVTGGELGYWNPIPTLTWSDLPTTNGAIQTQYQ