AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene87356

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene87356.t1 MTR_8g079060 86.777 242 19 5 1 242 1 229 2.84e-123 349
MS.gene87356.t1 MTR_8g015840 74.286 70 17 1 17 85 7 76 5.18e-32 117
MS.gene87356.t1 MTR_2g096740 65.333 75 26 0 22 96 55 129 1.47e-31 117
MS.gene87356.t1 MTR_2g096740 65.333 75 26 0 22 96 70 144 1.71e-31 117
MS.gene87356.t1 MTR_8g027295 42.553 141 79 1 23 163 18 156 1.15e-30 114
MS.gene87356.t1 MTR_4g063780 51.887 106 38 2 17 109 54 159 1.20e-30 116
MS.gene87356.t1 MTR_4g088580 63.158 76 28 0 31 106 57 132 1.21e-30 117
MS.gene87356.t1 MTR_8g479350 59.494 79 32 0 26 104 65 143 7.45e-30 114
MS.gene87356.t1 MTR_7g059400 44.805 154 52 4 17 142 55 203 1.25e-29 114
MS.gene87356.t1 MTR_2g093220 41.916 167 90 3 12 174 24 187 2.35e-29 112
MS.gene87356.t1 MTR_4g461080 61.728 81 23 1 1 81 9 81 3.78e-29 110
MS.gene87356.t1 MTR_4g022370 40.217 184 87 6 17 190 61 231 4.51e-29 112
MS.gene87356.t1 MTR_7g024670 75.410 61 15 0 31 91 79 139 6.66e-29 112
MS.gene87356.t1 MTR_1g077600 62.857 70 26 0 14 83 28 97 5.32e-28 108
MS.gene87356.t1 MTR_1g077600 62.857 70 26 0 14 83 28 97 5.69e-28 107
MS.gene87356.t1 MTR_3g091820 70.588 68 18 2 18 85 33 98 8.92e-28 108
MS.gene87356.t1 MTR_4g089095 75.000 60 14 1 27 86 48 106 2.96e-27 106
MS.gene87356.t1 MTR_4g089095 78.947 57 11 1 27 83 14 69 4.02e-27 105
MS.gene87356.t1 MTR_3g435480 54.023 87 39 1 29 114 136 222 5.30e-27 108
MS.gene87356.t1 MTR_5g031440 71.429 56 16 0 24 79 38 93 1.14e-26 105
MS.gene87356.t1 MTR_8g068210 54.321 81 34 1 29 109 32 109 2.39e-26 104
MS.gene87356.t1 MTR_2g013370 75.439 57 13 1 27 83 44 99 3.02e-26 103
MS.gene87356.t1 MTR_2g014170 83.673 49 8 0 29 77 45 93 5.61e-26 103
MS.gene87356.t1 MTR_4g109980 79.245 53 11 0 27 79 38 90 6.31e-26 103
MS.gene87356.t1 MTR_3g077750 70.312 64 15 1 16 79 52 111 6.84e-26 103
MS.gene87356.t1 MTR_7g010950 38.065 155 71 4 29 178 135 269 7.89e-26 105
MS.gene87356.t1 MTR_2g014060 61.972 71 23 1 10 80 10 76 8.31e-26 103
MS.gene87356.t1 MTR_2g059540 81.633 49 9 0 29 77 31 79 5.33e-25 98.2
MS.gene87356.t1 MTR_4g082060 63.934 61 22 0 29 89 122 182 5.57e-25 102
MS.gene87356.t1 MTR_3g090430 56.098 82 26 1 2 83 34 105 7.34e-25 100
MS.gene87356.t1 MTR_6g012450 37.662 154 76 4 29 176 136 275 8.35e-25 102
MS.gene87356.t1 MTR_2g016030 67.273 55 18 0 26 80 44 98 1.55e-24 95.9
MS.gene87356.t1 MTR_5g041420 53.659 82 38 0 21 102 130 211 2.46e-24 99.0
MS.gene87356.t1 MTR_8g044220 60.606 66 25 1 29 93 118 183 3.19e-24 100
MS.gene87356.t1 MTR_1g056810 59.211 76 29 1 31 106 14 87 5.19e-24 97.8
MS.gene87356.t1 MTR_7g086780 40.517 116 62 3 31 139 101 216 1.22e-23 98.6
MS.gene87356.t1 MTR_6g027460 39.695 131 73 3 29 156 96 223 2.58e-23 97.1
MS.gene87356.t1 MTR_5g041380 73.585 53 14 0 29 81 127 179 3.94e-23 96.7
MS.gene87356.t1 MTR_5g041400 73.585 53 14 0 29 81 119 171 4.56e-23 96.3
MS.gene87356.t1 MTR_5g041530 62.295 61 23 0 21 81 132 192 1.72e-22 95.1
MS.gene87356.t1 MTR_6g027450 56.716 67 28 1 29 94 89 155 1.99e-22 94.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene87356.t1 AT3G50410 48.289 263 105 9 1 242 1 253 6.46e-59 187
MS.gene87356.t1 AT5G66940 46.186 236 94 9 4 223 3 221 5.89e-50 163
MS.gene87356.t1 AT1G51700 47.561 164 53 6 24 171 28 174 1.54e-35 125
MS.gene87356.t1 AT3G21270 43.293 164 76 5 29 184 29 183 8.14e-33 118
MS.gene87356.t1 AT5G60850 66.265 83 25 2 27 109 49 128 1.36e-31 118
MS.gene87356.t1 AT5G60200 63.636 88 24 2 22 109 48 127 7.94e-31 115
MS.gene87356.t1 AT3G45610 52.294 109 43 2 2 110 13 112 8.74e-31 114
MS.gene87356.t1 AT1G47655 39.316 234 107 7 11 242 9 209 4.79e-30 111
MS.gene87356.t1 AT5G65590 68.182 66 21 0 23 88 35 100 1.18e-28 110
MS.gene87356.t1 AT1G07640 74.138 58 15 0 29 86 21 78 1.98e-28 109
MS.gene87356.t1 AT1G07640 74.138 58 15 0 29 86 77 134 3.73e-28 109
MS.gene87356.t1 AT3G55370 63.514 74 25 2 21 93 114 186 3.92e-28 110
MS.gene87356.t1 AT1G07640 74.138 58 15 0 29 86 85 142 4.71e-28 109
MS.gene87356.t1 AT3G55370 63.514 74 25 2 21 93 69 141 5.53e-28 108
MS.gene87356.t1 AT3G55370 63.514 74 25 2 21 93 69 141 5.87e-28 109
MS.gene87356.t1 AT1G28310 75.862 58 14 0 29 86 27 84 6.46e-28 108
MS.gene87356.t1 AT5G62430 63.158 76 27 1 29 103 54 129 7.67e-28 108
MS.gene87356.t1 AT1G28310 75.862 58 14 0 29 86 51 108 8.12e-28 108
MS.gene87356.t1 AT3G61850 70.769 65 18 1 23 87 56 119 3.00e-27 106
MS.gene87356.t1 AT3G61850 70.769 65 18 1 23 87 56 119 3.00e-27 106
MS.gene87356.t1 AT3G61850 70.769 65 18 1 23 87 68 131 3.49e-27 106
MS.gene87356.t1 AT4G24060 52.083 96 43 2 18 111 43 137 3.69e-27 107
MS.gene87356.t1 AT5G39660 56.098 82 34 1 29 108 138 219 3.90e-27 108
MS.gene87356.t1 AT5G39660 56.098 82 34 1 29 108 138 219 3.90e-27 108
MS.gene87356.t1 AT5G39660 56.098 82 34 1 29 108 138 219 3.90e-27 108
MS.gene87356.t1 AT5G02460 63.636 77 17 2 17 83 74 149 3.91e-27 107
MS.gene87356.t1 AT3G61850 70.769 65 18 1 23 87 56 119 5.09e-27 106
MS.gene87356.t1 AT3G61850 70.769 65 18 1 23 87 68 131 5.22e-27 106
MS.gene87356.t1 AT3G47500 40.741 135 64 3 1 121 84 216 5.73e-27 108
MS.gene87356.t1 AT4G38000 61.039 77 27 2 10 83 19 95 7.67e-27 104
MS.gene87356.t1 AT2G28810 73.684 57 15 0 24 80 72 128 8.09e-27 105
MS.gene87356.t1 AT2G28810 73.684 57 15 0 24 80 89 145 1.08e-26 105
MS.gene87356.t1 AT2G37590 59.722 72 29 0 12 83 72 143 4.03e-26 103
MS.gene87356.t1 AT2G28510 74.545 55 14 0 31 85 50 104 9.07e-26 102
MS.gene87356.t1 AT4G21050 64.179 67 23 1 31 97 26 91 9.99e-26 100
MS.gene87356.t1 AT1G21340 64.706 68 21 1 25 89 31 98 1.35e-25 101
MS.gene87356.t1 AT4G00940 70.690 58 17 0 26 83 65 122 3.80e-25 100
MS.gene87356.t1 AT4G00940 70.690 58 17 0 26 83 65 122 3.80e-25 100
MS.gene87356.t1 AT4G00940 70.690 58 17 0 26 83 65 122 3.80e-25 100
MS.gene87356.t1 AT4G21040 64.179 67 22 1 31 97 27 91 9.76e-25 98.6
MS.gene87356.t1 AT1G69570 62.687 67 24 1 29 94 132 198 1.03e-24 101
MS.gene87356.t1 AT5G62940 67.188 64 17 1 16 79 64 123 1.16e-24 100
MS.gene87356.t1 AT3G52440 63.636 66 24 0 18 83 14 79 1.18e-24 98.6
MS.gene87356.t1 AT2G46590 74.074 54 14 0 23 76 62 115 1.52e-24 100
MS.gene87356.t1 AT3G52440 63.636 66 24 0 18 83 33 98 1.58e-24 98.6
MS.gene87356.t1 AT1G29160 45.000 100 55 0 29 128 62 161 1.66e-24 96.3
MS.gene87356.t1 AT2G46590 74.074 54 14 0 23 76 74 127 1.74e-24 100
MS.gene87356.t1 AT1G26790 71.698 53 15 0 29 81 111 163 1.96e-24 100
MS.gene87356.t1 AT4G21080 65.672 67 21 1 31 97 27 91 3.30e-24 97.4
MS.gene87356.t1 AT1G64620 60.811 74 20 2 14 78 25 98 3.43e-23 96.7
MS.gene87356.t1 AT2G34140 67.925 53 17 0 29 81 58 110 6.33e-23 92.0
MS.gene87356.t1 AT4G21030 43.750 80 39 1 31 110 23 96 5.13e-14 68.9

Find 91 sgRNAs with CRISPR-Local

Find 102 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTCTTGCATTAGGTGGATT+TGG 0.219728 8.3:+25227689 MS.gene87356:CDS
GCAAAGCTTGTCGCCGTTAC+TGG 0.222236 8.3:+25227378 MS.gene87356:CDS
CCGTCAATACCGCCGGAAAA+TGG 0.237022 8.3:-25227590 None:intergenic
GGTGTTCCTGTTGTGTCTTC+TGG 0.239637 8.3:+25227803 MS.gene87356:CDS
GCATTAGGTGGATTTGGGCT+TGG 0.275419 8.3:+25227695 MS.gene87356:CDS
TTCTTGCATTAGGTGGATTT+GGG 0.281448 8.3:+25227690 MS.gene87356:CDS
CATTAGGTGGATTTGGGCTT+GGG 0.284647 8.3:+25227696 MS.gene87356:CDS
GCTATTTCAACACCAGTAAA+TGG 0.284985 8.3:+25227908 MS.gene87356:CDS
GGTGGATTTGGGCTTGGGCT+TGG 0.292816 8.3:+25227701 MS.gene87356:CDS
TTTGGGCTTGGGCTTGGACA+TGG 0.300293 8.3:+25227707 MS.gene87356:CDS
TTGGGCTTGGGCTTGGACAT+GGG 0.342240 8.3:+25227708 MS.gene87356:CDS
TTTGATCCATCCACCATTCC+TGG 0.347481 8.3:-25227776 None:intergenic
GGGCTTGAAGAAATGGGCTT+TGG 0.347490 8.3:+25227728 MS.gene87356:CDS
GTTGTGTCTTCTGGTGTTGT+TGG 0.348865 8.3:+25227812 MS.gene87356:CDS
AAGAAATGGGCTTTGGGATT+GGG 0.351153 8.3:+25227735 MS.gene87356:CDS
TGCTCCGGTGCCGGTGCACC+GGG 0.353622 8.3:-25227266 None:intergenic
TGGACATGGGCTTGAAGAAA+TGG 0.357925 8.3:+25227721 MS.gene87356:CDS
TGGGATTGGGAGAACAGGTT+GGG 0.361604 8.3:+25227748 MS.gene87356:CDS
TCCACCTTGGACGGCGGAGA+TGG 0.366306 8.3:-25227558 None:intergenic
GGTGATTGTTTCTCTTGGCC+TGG 0.369523 8.3:+25227881 MS.gene87356:CDS
GGCTTTGGGATTGGGAGAAC+AGG 0.373009 8.3:+25227743 MS.gene87356:CDS
TTGCTCCGGTGCCGGTGCAC+CGG 0.375742 8.3:-25227267 None:intergenic
GGCTTGAAGAAATGGGCTTT+GGG 0.379257 8.3:+25227729 MS.gene87356:CDS
GTTCATTTGAGACCATTTAC+TGG 0.380286 8.3:-25227920 None:intergenic
GAAGAAATGGGCTTTGGGAT+TGG 0.382875 8.3:+25227734 MS.gene87356:CDS
GTGGTGGTGATTGTTTCTCT+TGG 0.384765 8.3:+25227876 MS.gene87356:CDS
ATCTCCGCCGTCCAAGGTGG+AGG 0.395663 8.3:+25227560 MS.gene87356:CDS
AGCTCTGGGTTTCTTGCATT+AGG 0.397585 8.3:+25227680 MS.gene87356:CDS
CGCCGTGACTTCAGCGGCGC+CGG 0.403138 8.3:+25227508 MS.gene87356:CDS
TGTTGATCGGCGGCTTTCAC+CGG 0.408674 8.3:-25227228 None:intergenic
GGTGCACCGGGGCGGATTTG+AGG 0.417132 8.3:-25227254 None:intergenic
AACAACAACAACCAAAGCTC+TGG 0.420136 8.3:+25227665 MS.gene87356:CDS
CCGCCCCGGTGCACCGGCAC+CGG 0.422541 8.3:+25227262 MS.gene87356:CDS
ATGCAAGAAACCCAGAGCTT+TGG 0.423678 8.3:-25227676 None:intergenic
CCACCTTGGACGGCGGAGAT+GGG 0.436586 8.3:-25227557 None:intergenic
AGATTCTCTTGCTCCGGTGC+CGG 0.436640 8.3:-25227275 None:intergenic
GGACATGGGCTTGAAGAAAT+GGG 0.438047 8.3:+25227722 MS.gene87356:CDS
ATGGTTAGTACGAGAGCGTT+TGG 0.439453 8.3:-25227456 None:intergenic
TTGGGATTGGGAGAACAGGT+TGG 0.446014 8.3:+25227747 MS.gene87356:CDS
CTCCGGCGCCGCTGAAGTCA+CGG 0.453189 8.3:-25227510 None:intergenic
ACAACACCAGAAGACACAAC+AGG 0.458334 8.3:-25227809 None:intergenic
CCGGCGGTATTGACGGTGAT+GGG 0.466545 8.3:+25227597 MS.gene87356:CDS
GGAAACACGTGGCAGTTAGA+AGG 0.471025 8.3:+25227833 MS.gene87356:CDS
TTTCTGGTACCACCGCCGAC+GGG 0.484650 8.3:-25227428 None:intergenic
CGTGACATCCCCGTCGGCGG+TGG 0.490474 8.3:+25227419 MS.gene87356:CDS
CATGGGAGATTCTCTTGCTC+CGG 0.492080 8.3:-25227281 None:intergenic
CAGTTAGAAGGTGGTGAAGG+TGG 0.495978 8.3:+25227845 MS.gene87356:CDS
TCTCTAAACAATGACATCTT+CGG 0.498140 8.3:+25227202 None:intergenic
TCAAATCCGCCCCGGTGCAC+CGG 0.515212 8.3:+25227256 MS.gene87356:CDS
TCCGGCGGTATTGACGGTGA+TGG 0.517628 8.3:+25227596 MS.gene87356:CDS
CGCCGCTGAAGTCACGGCGG+AGG 0.518030 8.3:-25227504 None:intergenic
AAGCAGAATATGAGTGTTTG+TGG 0.522514 8.3:+25227620 MS.gene87356:CDS
TCTGGGTTTCTTGCATTAGG+TGG 0.528875 8.3:+25227683 MS.gene87356:CDS
GCTTGATAGTGGTGGTGCTC+CGG 0.532605 8.3:-25227527 None:intergenic
GTTGAATCACAACGTGGACA+TGG 0.538502 8.3:-25227299 None:intergenic
CACCGCCGACGGGGATGTCA+CGG 0.552285 8.3:-25227418 None:intergenic
GTAGTAACAAAACTTAGTGT+TGG 0.560310 8.3:-25227321 None:intergenic
CACGGCGGAGGAAGAAGATG+AGG 0.560822 8.3:-25227492 None:intergenic
ACACCCATCTCCGCCGTCCA+AGG 0.562889 8.3:+25227554 MS.gene87356:CDS
TCTCCGCCGTCCAAGGTGGA+GGG 0.563713 8.3:+25227561 MS.gene87356:CDS
TTCTGGTACCACCGCCGACG+GGG 0.573655 8.3:-25227427 None:intergenic
ATGGAAAAGACCCTCCACCT+TGG 0.576601 8.3:-25227571 None:intergenic
CCCATCACCGTCAATACCGC+CGG 0.580375 8.3:-25227597 None:intergenic
ACAATGACATCTTCGGACTC+CGG 0.583202 8.3:+25227209 None:intergenic
CTCCGTGACATCCCCGTCGG+CGG 0.592048 8.3:+25227416 MS.gene87356:CDS
AAAAGACCCTCCACCTTGGA+CGG 0.592275 8.3:-25227567 None:intergenic
GGTGTTGAAATAGCAAGTCC+AGG 0.592777 8.3:-25227899 None:intergenic
ACAACAACAACCAAAGCTCT+GGG 0.596408 8.3:+25227666 MS.gene87356:CDS
TGGGTGTCATTGCTTGATAG+TGG 0.598785 8.3:-25227538 None:intergenic
TTCCACCGTGAGTCCAGTAA+CGG 0.599141 8.3:-25227391 None:intergenic
TTCCTCCGCCGTGACTTCAG+CGG 0.607903 8.3:+25227502 MS.gene87356:CDS
CCGGTGCCGGTGCACCGGGG+CGG 0.610074 8.3:-25227262 None:intergenic
CCCATCTCCGCCGTCCAAGG+TGG 0.613450 8.3:+25227557 MS.gene87356:CDS
ACAAAACCTCAAATCCGCCC+CGG 0.621584 8.3:+25227248 MS.gene87356:CDS
TGTCGCCGTTACTGGACTCA+CGG 0.630187 8.3:+25227386 MS.gene87356:CDS
AGACCCTCCACCTTGGACGG+CGG 0.634960 8.3:-25227564 None:intergenic
TGGCAGTTAGAAGGTGGTGA+AGG 0.635231 8.3:+25227842 MS.gene87356:CDS
ATGAGGAAGTTACAGCAACA+TGG 0.636291 8.3:-25227475 None:intergenic
ACCCTCCGTGACATCCCCGT+CGG 0.636867 8.3:+25227413 MS.gene87356:CDS
CAAGCTTTGCAGAAATGACG+TGG 0.636873 8.3:-25227365 None:intergenic
CGCCGACGGGGATGTCACGG+AGG 0.643952 8.3:-25227415 None:intergenic
TTGAATCACAACGTGGACAT+GGG 0.644805 8.3:-25227298 None:intergenic
GCCGACGGGGATGTCACGGA+GGG 0.646087 8.3:-25227414 None:intergenic
GTGTCATTGCTTGATAGTGG+TGG 0.649877 8.3:-25227535 None:intergenic
GCTCCGGTGCCGGTGCACCG+GGG 0.657998 8.3:-25227265 None:intergenic
AACACGTGGCAGTTAGAAGG+TGG 0.673819 8.3:+25227836 MS.gene87356:CDS
CGCCGTTACTGGACTCACGG+TGG 0.693801 8.3:+25227389 MS.gene87356:CDS
ATGGTGGATGGATCAAACAT+TGG 0.699118 8.3:+25227782 MS.gene87356:CDS
CTGGTGTTGTTGGAAACACG+TGG 0.703407 8.3:+25227822 MS.gene87356:CDS
CGGCGCCGCTGAAGTCACGG+CGG 0.765762 8.3:-25227507 None:intergenic
GTGTTGGTTGAATCACAACG+TGG 0.795975 8.3:-25227305 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
GTAGTAACAAAACTTAGTGT+TGG - chr8.3:25227324-25227343 None:intergenic 30.0%
AAGCAGAATATGAGTGTTTG+TGG + chr8.3:25227620-25227639 MS.gene87356:CDS 35.0%
! GCTATTTCAACACCAGTAAA+TGG + chr8.3:25227908-25227927 MS.gene87356:CDS 35.0%
! TTCTTGCATTAGGTGGATTT+GGG + chr8.3:25227690-25227709 MS.gene87356:CDS 35.0%
! TTTCTTGCATTAGGTGGATT+TGG + chr8.3:25227689-25227708 MS.gene87356:CDS 35.0%
!!! GGATTTGAGGTTTTGTTGAT+CGG - chr8.3:25227244-25227263 None:intergenic 35.0%
AACAACAACAACCAAAGCTC+TGG + chr8.3:25227665-25227684 MS.gene87356:CDS 40.0%
ACAACAACAACCAAAGCTCT+GGG + chr8.3:25227666-25227685 MS.gene87356:CDS 40.0%
ATGAGGAAGTTACAGCAACA+TGG - chr8.3:25227478-25227497 None:intergenic 40.0%
TTGAATCACAACGTGGACAT+GGG - chr8.3:25227301-25227320 None:intergenic 40.0%
!! AAGAAATGGGCTTTGGGATT+GGG + chr8.3:25227735-25227754 MS.gene87356:CDS 40.0%
!! ATGGTGGATGGATCAAACAT+TGG + chr8.3:25227782-25227801 MS.gene87356:CDS 40.0%
!!! TTTGAGGTTTTGTTGATCGG+CGG - chr8.3:25227241-25227260 None:intergenic 40.0%
ACAACACCAGAAGACACAAC+AGG - chr8.3:25227812-25227831 None:intergenic 45.0%
ATGCAAGAAACCCAGAGCTT+TGG - chr8.3:25227679-25227698 None:intergenic 45.0%
CAAGCTTTGCAGAAATGACG+TGG - chr8.3:25227368-25227387 None:intergenic 45.0%
GGACATGGGCTTGAAGAAAT+GGG + chr8.3:25227722-25227741 MS.gene87356:CDS 45.0%
GTTGAATCACAACGTGGACA+TGG - chr8.3:25227302-25227321 None:intergenic 45.0%
TCTGGGTTTCTTGCATTAGG+TGG + chr8.3:25227683-25227702 MS.gene87356:CDS 45.0%
TGGACATGGGCTTGAAGAAA+TGG + chr8.3:25227721-25227740 MS.gene87356:CDS 45.0%
TTTGATCCATCCACCATTCC+TGG - chr8.3:25227779-25227798 None:intergenic 45.0%
! CATTAGGTGGATTTGGGCTT+GGG + chr8.3:25227696-25227715 MS.gene87356:CDS 45.0%
! GGAGGGTCTTTTCCATTTTC+CGG + chr8.3:25227578-25227597 MS.gene87356:CDS 45.0%
! GGCTTGAAGAAATGGGCTTT+GGG + chr8.3:25227729-25227748 MS.gene87356:CDS 45.0%
! GGTGTTGAAATAGCAAGTCC+AGG - chr8.3:25227902-25227921 None:intergenic 45.0%
! GTGGTGGTGATTGTTTCTCT+TGG + chr8.3:25227876-25227895 MS.gene87356:CDS 45.0%
!! AGCTCTGGGTTTCTTGCATT+AGG + chr8.3:25227680-25227699 MS.gene87356:CDS 45.0%
!! ATGGTTAGTACGAGAGCGTT+TGG - chr8.3:25227459-25227478 None:intergenic 45.0%
!! GAAGAAATGGGCTTTGGGAT+TGG + chr8.3:25227734-25227753 MS.gene87356:CDS 45.0%
!! GTGTCATTGCTTGATAGTGG+TGG - chr8.3:25227538-25227557 None:intergenic 45.0%
!! GTGTTGGTTGAATCACAACG+TGG - chr8.3:25227308-25227327 None:intergenic 45.0%
!! GTTGTGTCTTCTGGTGTTGT+TGG + chr8.3:25227812-25227831 MS.gene87356:CDS 45.0%
!! TGGGTGTCATTGCTTGATAG+TGG - chr8.3:25227541-25227560 None:intergenic 45.0%
!!! AGAGCGTTTGGCGTTTTTTC+TGG - chr8.3:25227447-25227466 None:intergenic 45.0%
AAAAGACCCTCCACCTTGGA+CGG - chr8.3:25227570-25227589 None:intergenic 50.0%
AACACGTGGCAGTTAGAAGG+TGG + chr8.3:25227836-25227855 MS.gene87356:CDS 50.0%
ACAAAACCTCAAATCCGCCC+CGG + chr8.3:25227248-25227267 MS.gene87356:CDS 50.0%
ATGGAAAAGACCCTCCACCT+TGG - chr8.3:25227574-25227593 None:intergenic 50.0%
CAGTTAGAAGGTGGTGAAGG+TGG + chr8.3:25227845-25227864 MS.gene87356:CDS 50.0%
CATGGGAGATTCTCTTGCTC+CGG - chr8.3:25227284-25227303 None:intergenic 50.0%
GGAAACACGTGGCAGTTAGA+AGG + chr8.3:25227833-25227852 MS.gene87356:CDS 50.0%
GGTGTTCCTGTTGTGTCTTC+TGG + chr8.3:25227803-25227822 MS.gene87356:CDS 50.0%
TGGCAGTTAGAAGGTGGTGA+AGG + chr8.3:25227842-25227861 MS.gene87356:CDS 50.0%
TGGGATTGGGAGAACAGGTT+GGG + chr8.3:25227748-25227767 MS.gene87356:CDS 50.0%
TTCCACCGTGAGTCCAGTAA+CGG - chr8.3:25227394-25227413 None:intergenic 50.0%
TTGGGATTGGGAGAACAGGT+TGG + chr8.3:25227747-25227766 MS.gene87356:CDS 50.0%
! AGGTTGGGCTTTTCCAGGAA+TGG + chr8.3:25227763-25227782 MS.gene87356:CDS 50.0%
! GCATTAGGTGGATTTGGGCT+TGG + chr8.3:25227695-25227714 MS.gene87356:CDS 50.0%
! GGGCTTGAAGAAATGGGCTT+TGG + chr8.3:25227728-25227747 MS.gene87356:CDS 50.0%
! GGGTCTTTTCCATTTTCCGG+CGG + chr8.3:25227581-25227600 MS.gene87356:CDS 50.0%
! GGTGATTGTTTCTCTTGGCC+TGG + chr8.3:25227881-25227900 MS.gene87356:CDS 50.0%
! TTGGGCTTTTCCAGGAATGG+TGG + chr8.3:25227766-25227785 MS.gene87356:CDS 50.0%
!! AGAACAGGTTGGGCTTTTCC+AGG + chr8.3:25227758-25227777 MS.gene87356:CDS 50.0%
!! CCATTTTCCGGCGGTATTGA+CGG + chr8.3:25227590-25227609 MS.gene87356:CDS 50.0%
!! CTGGTGTTGTTGGAAACACG+TGG + chr8.3:25227822-25227841 MS.gene87356:CDS 50.0%
!! GCTTTTCCAGGAATGGTGGA+TGG + chr8.3:25227770-25227789 MS.gene87356:CDS 50.0%
!!! GAAGGTGGTTTTGTTGGTGG+TGG + chr8.3:25227860-25227879 MS.gene87356:CDS 50.0%
!!! GGTGAAGGTGGTTTTGTTGG+TGG + chr8.3:25227857-25227876 MS.gene87356:CDS 50.0%
!!! GGTGGTGAAGGTGGTTTTGT+TGG + chr8.3:25227854-25227873 MS.gene87356:CDS 50.0%
AGATTCTCTTGCTCCGGTGC+CGG - chr8.3:25227278-25227297 None:intergenic 55.0%
CCGTCAATACCGCCGGAAAA+TGG - chr8.3:25227593-25227612 None:intergenic 55.0%
GCAAAGCTTGTCGCCGTTAC+TGG + chr8.3:25227378-25227397 MS.gene87356:CDS 55.0%
TGTCGCCGTTACTGGACTCA+CGG + chr8.3:25227386-25227405 MS.gene87356:CDS 55.0%
TGTTGATCGGCGGCTTTCAC+CGG - chr8.3:25227231-25227250 None:intergenic 55.0%
TTGGGCTTGGGCTTGGACAT+GGG + chr8.3:25227708-25227727 MS.gene87356:CDS 55.0%
TTTGGGCTTGGGCTTGGACA+TGG + chr8.3:25227707-25227726 MS.gene87356:CDS 55.0%
!! GCTTGATAGTGGTGGTGCTC+CGG - chr8.3:25227530-25227549 None:intergenic 55.0%
!! GGCTTTGGGATTGGGAGAAC+AGG + chr8.3:25227743-25227762 MS.gene87356:CDS 55.0%
!! TTTTCTGGTACCACCGCCGA+CGG - chr8.3:25227432-25227451 None:intergenic 55.0%
CACGGCGGAGGAAGAAGATG+AGG - chr8.3:25227495-25227514 None:intergenic 60.0%
CCCATCACCGTCAATACCGC+CGG - chr8.3:25227600-25227619 None:intergenic 60.0%
TTCCTCCGCCGTGACTTCAG+CGG + chr8.3:25227502-25227521 MS.gene87356:CDS 60.0%
! GGTGGATTTGGGCTTGGGCT+TGG + chr8.3:25227701-25227720 MS.gene87356:CDS 60.0%
! TTTCTGGTACCACCGCCGAC+GGG - chr8.3:25227431-25227450 None:intergenic 60.0%
!! CCGGCGGTATTGACGGTGAT+GGG + chr8.3:25227597-25227616 MS.gene87356:CDS 60.0%
!! TCCGGCGGTATTGACGGTGA+TGG + chr8.3:25227596-25227615 MS.gene87356:CDS 60.0%
ACACCCATCTCCGCCGTCCA+AGG + chr8.3:25227554-25227573 MS.gene87356:CDS 65.0%
ACCCTCCGTGACATCCCCGT+CGG + chr8.3:25227413-25227432 MS.gene87356:CDS 65.0%
AGACCCTCCACCTTGGACGG+CGG - chr8.3:25227567-25227586 None:intergenic 65.0%
ATCTCCGCCGTCCAAGGTGG+AGG + chr8.3:25227560-25227579 MS.gene87356:CDS 65.0%
CCACCTTGGACGGCGGAGAT+GGG - chr8.3:25227560-25227579 None:intergenic 65.0%
CGCCGTTACTGGACTCACGG+TGG + chr8.3:25227389-25227408 MS.gene87356:CDS 65.0%
TCAAATCCGCCCCGGTGCAC+CGG + chr8.3:25227256-25227275 MS.gene87356:CDS 65.0%
TCCACCTTGGACGGCGGAGA+TGG - chr8.3:25227561-25227580 None:intergenic 65.0%
TCTCCGCCGTCCAAGGTGGA+GGG + chr8.3:25227561-25227580 MS.gene87356:CDS 65.0%
TTCTGGTACCACCGCCGACG+GGG - chr8.3:25227430-25227449 None:intergenic 65.0%
CACCGCCGACGGGGATGTCA+CGG - chr8.3:25227421-25227440 None:intergenic 70.0%
CCCATCTCCGCCGTCCAAGG+TGG + chr8.3:25227557-25227576 MS.gene87356:CDS 70.0%
CTCCGGCGCCGCTGAAGTCA+CGG - chr8.3:25227513-25227532 None:intergenic 70.0%
CTCCGTGACATCCCCGTCGG+CGG + chr8.3:25227416-25227435 MS.gene87356:CDS 70.0%
GCCGACGGGGATGTCACGGA+GGG - chr8.3:25227417-25227436 None:intergenic 70.0%
! GGTGCACCGGGGCGGATTTG+AGG - chr8.3:25227257-25227276 None:intergenic 70.0%
!! TTGCTCCGGTGCCGGTGCAC+CGG - chr8.3:25227270-25227289 None:intergenic 70.0%
CGCCGACGGGGATGTCACGG+AGG - chr8.3:25227418-25227437 None:intergenic 75.0%
CGCCGCTGAAGTCACGGCGG+AGG - chr8.3:25227507-25227526 None:intergenic 75.0%
CGCCGTGACTTCAGCGGCGC+CGG + chr8.3:25227508-25227527 MS.gene87356:CDS 75.0%
CGGCGCCGCTGAAGTCACGG+CGG - chr8.3:25227510-25227529 None:intergenic 75.0%
CGTGACATCCCCGTCGGCGG+TGG + chr8.3:25227419-25227438 MS.gene87356:CDS 75.0%
!! TGCTCCGGTGCCGGTGCACC+GGG - chr8.3:25227269-25227288 None:intergenic 75.0%
!!! GCTCCGGTGCCGGTGCACCG+GGG - chr8.3:25227268-25227287 None:intergenic 80.0%
!! CCGCCCCGGTGCACCGGCAC+CGG + chr8.3:25227262-25227281 MS.gene87356:CDS 85.0%
!!! CCGGTGCCGGTGCACCGGGG+CGG - chr8.3:25227265-25227284 None:intergenic 85.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 25227212 25227940 25227212 ID=MS.gene87356
chr8.3 mRNA 25227212 25227940 25227212 ID=MS.gene87356.t1;Parent=MS.gene87356
chr8.3 exon 25227212 25227940 25227212 ID=MS.gene87356.t1.exon1;Parent=MS.gene87356.t1
chr8.3 CDS 25227212 25227940 25227212 ID=cds.MS.gene87356.t1;Parent=MS.gene87356.t1
Gene Sequence

>MS.gene87356

ATGACATCTTCGGACTCCGGTGAAAGCCGCCGATCAACAAAACCTCAAATCCGCCCCGGTGCACCGGCACCGGAGCAAGAGAATCTCCCATGTCCACGTTGTGATTCAACCAACACTAAGTTTTGTTACTACAACAACTACAACTACTCTCAGCCACGTCATTTCTGCAAAGCTTGTCGCCGTTACTGGACTCACGGTGGAACCCTCCGTGACATCCCCGTCGGCGGTGGTACCAGAAAAAACGCCAAACGCTCTCGTACTAACCATGTTGCTGTAACTTCCTCATCTTCTTCCTCCGCCGTGACTTCAGCGGCGCCGGAGCACCACCACTATCAAGCAATGACACCCATCTCCGCCGTCCAAGGTGGAGGGTCTTTTCCATTTTCCGGCGGTATTGACGGTGATGGGAAGCAGAATATGAGTGTTTGTGGAAGCTTCACTTCATTGCTGAACAACAACAACAACCAAAGCTCTGGGTTTCTTGCATTAGGTGGATTTGGGCTTGGGCTTGGACATGGGCTTGAAGAAATGGGCTTTGGGATTGGGAGAACAGGTTGGGCTTTTCCAGGAATGGTGGATGGATCAAACATTGGTGTTCCTGTTGTGTCTTCTGGTGTTGTTGGAAACACGTGGCAGTTAGAAGGTGGTGAAGGTGGTTTTGTTGGTGGTGGTGATTGTTTCTCTTGGCCTGGACTTGCTATTTCAACACCAGTAAATGGTCTCAAATGA
Protein sequence

>MS.gene87356.t1

MTSSDSGESRRSTKPQIRPGAPAPEQENLPCPRCDSTNTKFCYYNNYNYSQPRHFCKACRRYWTHGGTLRDIPVGGGTRKNAKRSRTNHVAVTSSSSSSAVTSAAPEHHHYQAMTPISAVQGGGSFPFSGGIDGDGKQNMSVCGSFTSLLNNNNNQSSGFLALGGFGLGLGHGLEEMGFGIGRTGWAFPGMVDGSNIGVPVVSSGVVGNTWQLEGGEGGFVGGGDCFSWPGLAISTPVNGLK