AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene87409

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene87409.t1 MTR_8g077940 98.556 277 4 0 53 329 136 412 0.0 527
MS.gene87409.t1 MTR_0092s0100 36.017 236 143 6 97 329 373 603 1.57e-33 132
MS.gene87409.t1 MTR_0092s0100 36.017 236 143 6 97 329 370 600 1.71e-33 132
MS.gene87409.t1 MTR_5g019750 57.407 108 45 1 223 329 59 166 3.68e-32 123
MS.gene87409.t1 MTR_4g026485 39.344 183 105 5 97 277 267 445 1.96e-29 120
MS.gene87409.t1 MTR_5g058860 29.615 260 163 8 96 337 116 373 6.31e-19 89.0
MS.gene87409.t1 MTR_3g072710 29.461 241 139 8 114 330 137 370 6.98e-15 76.6
MS.gene87409.t1 MTR_7g074650 26.939 245 162 9 102 340 441 674 5.06e-14 74.3
MS.gene87409.t1 MTR_3g065980 25.941 239 161 7 96 329 169 396 3.09e-13 71.6
MS.gene87409.t1 MTR_7g027190 24.521 261 165 9 91 329 292 542 5.39e-12 67.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene87409.t1 AT4G36710 54.225 284 116 6 67 347 98 370 4.36e-96 295
MS.gene87409.t1 AT4G00150 37.021 235 131 8 98 329 207 427 2.65e-35 136
MS.gene87409.t1 AT2G45160 29.880 251 154 8 97 329 269 515 1.43e-22 100
MS.gene87409.t1 AT3G60630 32.509 283 154 10 88 350 235 500 4.39e-21 95.5
MS.gene87409.t1 AT3G54220 26.939 245 165 8 102 340 296 532 2.28e-15 78.2
MS.gene87409.t1 AT4G08250 28.571 259 168 10 85 330 93 347 1.29e-13 72.8
MS.gene87409.t1 AT4G17230 27.358 212 125 7 75 265 124 327 1.31e-13 72.8
MS.gene87409.t1 AT4G17230 27.358 212 125 7 75 265 124 327 1.31e-13 72.8
MS.gene87409.t1 AT3G46600 25.210 238 155 8 112 329 214 448 2.17e-13 72.0
MS.gene87409.t1 AT3G46600 25.210 238 155 8 112 329 6 240 5.43e-13 70.1
MS.gene87409.t1 AT3G46600 25.210 238 155 8 112 329 182 416 6.32e-13 70.5
MS.gene87409.t1 AT5G59450 25.234 214 145 7 128 329 255 465 3.39e-12 68.6
MS.gene87409.t1 AT2G01570 29.252 147 97 4 96 242 221 360 3.38e-11 65.5
MS.gene87409.t1 AT1G21450 28.125 192 111 6 100 276 226 405 5.69e-11 64.7

Find 103 sgRNAs with CRISPR-Local

Find 170 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACACAACGTGGTGGTTGTT+GGG 0.276916 8.3:+26134928 None:intergenic
GTTGGGGTTGGTGTCGGGTT+GGG 0.288068 8.3:+26134945 None:intergenic
GGTAATTATAAGCCAGGTTT+TGG 0.301671 8.3:+26134610 None:intergenic
AAAGGCCTTGAAGGTTCTTA+TGG 0.314691 8.3:+26134284 None:intergenic
TGGTTGTTGGGGTTGGTGTC+GGG 0.344462 8.3:+26134940 None:intergenic
GGCTCGGTGTAGCGGTTTAC+CGG 0.345316 8.3:+26134401 None:intergenic
TCCAGATTAGTGTTTGTGTT+AGG 0.352738 8.3:+26134588 None:intergenic
GTGGTTGTTGGGGTTGGTGT+CGG 0.360358 8.3:+26134939 None:intergenic
TGACCTTGGAATTCGTGTTC+AGG 0.367914 8.3:-26134007 MS.gene87409:CDS
TTGCTATTGGTGTTGTTGTT+TGG 0.377424 8.3:+26134534 None:intergenic
TGTTGGGGTTGGTGTCGGGT+TGG 0.378037 8.3:+26134944 None:intergenic
CCACGGCGTCGTTCTTCTTC+CGG 0.380608 8.3:+26133615 None:intergenic
CCCTAACACAAACACTAATC+TGG 0.383350 8.3:-26134589 MS.gene87409:CDS
CCAGATTAGTGTTTGTGTTA+GGG 0.387299 8.3:+26134589 None:intergenic
TAACACAACGTGGTGGTTGT+TGG 0.387408 8.3:+26134927 None:intergenic
CGGTACGGCAGCGTTTCTTT+CGG 0.387789 8.3:-26133866 MS.gene87409:CDS
GATGAAATCCCAGTTGCTAT+TGG 0.391917 8.3:+26134521 None:intergenic
GTTGGTGTCGGGTTGGGAGT+TGG 0.396424 8.3:+26134951 None:intergenic
AAAATAGAAAACATTGGAAT+AGG 0.396620 8.3:+26134249 None:intergenic
ACAACAACACCAATAGCAAC+TGG 0.404968 8.3:-26134530 MS.gene87409:CDS
CAACTGCACCGGCCTCATTC+CGG 0.408407 8.3:+26133573 None:intergenic
TATTGGTGTTGTTGTTTGGT+TGG 0.426195 8.3:+26134538 None:intergenic
TCAAACCATAAGAACCTTCA+AGG 0.431138 8.3:-26134289 MS.gene87409:CDS
TCGTATTACCGCCGTCGTGC+CGG 0.439174 8.3:-26134085 MS.gene87409:CDS
CTCTTCTCTCCGGTTCAAAC+CGG 0.449911 8.3:-26134350 MS.gene87409:CDS
AATTCCAGAAAAGGCCTTGA+AGG 0.451575 8.3:+26134275 None:intergenic
TCCACCGCCGCAATTATCTT+CGG 0.453533 8.3:+26133643 None:intergenic
TGGATTAGTCCGGTTTGAAC+CGG 0.458396 8.3:+26134341 None:intergenic
TCAATACGCTTCTCTTATGA+AGG 0.459636 8.3:-26134145 MS.gene87409:CDS
CTTTCAACGGCGTACTCCTC+CGG 0.478029 8.3:+26134066 None:intergenic
CGGTGTCGTTTAAAGCGGTT+AGG 0.480818 8.3:-26133954 MS.gene87409:CDS
AACGTGGTGGTTGTTGGGGT+TGG 0.481466 8.3:+26134933 None:intergenic
AATCAGTTCGCGCATGACCT+TGG 0.482074 8.3:-26134021 MS.gene87409:CDS
GCCGAAGATAATTGCGGCGG+TGG 0.489687 8.3:-26133644 MS.gene87409:CDS
TGTTAGGGTAATTATAAGCC+AGG 0.491030 8.3:+26134604 None:intergenic
AATAGGTGAAATTCCAGAAA+AGG 0.509209 8.3:+26134266 None:intergenic
AGGAGTACGCCGTTGAAAGC+AGG 0.512249 8.3:-26134062 MS.gene87409:CDS
TCCACCGCGTCTTTCTTCCA+TGG 0.516075 8.3:-26134322 MS.gene87409:CDS
TTACGGCGCCGGAATGAGGC+CGG 0.517709 8.3:-26133581 MS.gene87409:CDS
AGCGGAGTGTTTGCTTGCGA+AGG 0.519373 8.3:-26133530 MS.gene87409:CDS
GATGAGGAAACCGCAGGAGG+AGG 0.527076 8.3:+26135029 None:intergenic
GCTCTTCAATCTCTTCTCTC+CGG 0.529069 8.3:-26134360 MS.gene87409:CDS
CTTGCGAAGGTGCAAATCAG+AGG 0.537232 8.3:-26133517 MS.gene87409:CDS
ATTGCGGCGGTGGAAGCTGC+CGG 0.538123 8.3:-26133634 MS.gene87409:CDS
AAAGACGCGGTGGATTAGTC+CGG 0.539736 8.3:+26134331 None:intergenic
CCGGCGATATTCTGTCGACT+TGG 0.540496 8.3:-26133895 MS.gene87409:CDS
GCAAAGCTTCGAGTAAAGCT+TGG 0.544163 8.3:+26134217 None:intergenic
CCGCCGCAATTATCTTCGGC+CGG 0.547383 8.3:+26133647 None:intergenic
CAGCGTTTCTTTCGGATGTG+AGG 0.549183 8.3:-26133858 MS.gene87409:CDS
CGGTGTAGCGGTTTACCGGT+GGG 0.550196 8.3:+26134405 None:intergenic
CAGCTTGTCGTTTCGCCACG+TGG 0.561710 8.3:+26133491 None:intergenic
TCGGTGTAGCGGTTTACCGG+TGG 0.563311 8.3:+26134404 None:intergenic
CCGTAAAACGCCTCTCTCCA+CGG 0.571296 8.3:+26133598 None:intergenic
CGTTTCTTTCGGATGTGAGG+AGG 0.571807 8.3:-26133855 MS.gene87409:CDS
TTCTGTCGACTTGGTTCCGA+CGG 0.575503 8.3:-26133886 MS.gene87409:CDS
GTGGTGGTGAGCTTTGGTTG+TGG 0.577306 8.3:+26134996 None:intergenic
TTACTCTAATAACCAAAACC+TGG 0.580137 8.3:-26134622 MS.gene87409:CDS
GCTTTACTCGAAGCTTTGCA+TGG 0.581206 8.3:-26134213 MS.gene87409:CDS
ACGAATCAACCTGCTTTCAA+CGG 0.586384 8.3:+26134053 None:intergenic
TGAAACGCGGCTCGGTGTAG+CGG 0.587607 8.3:+26134393 None:intergenic
TATTAGAGAGGCTGAATCAA+CGG 0.590000 8.3:-26134437 MS.gene87409:CDS
AAGAACGACGCCGTGGAGAG+AGG 0.590148 8.3:-26133608 MS.gene87409:CDS
TCGACTTGGTTCCGACGGTA+CGG 0.590389 8.3:-26133881 MS.gene87409:CDS
GGTGCAGTTGAGTCAGTTCG+CGG 0.593334 8.3:-26133560 MS.gene87409:CDS
GTACTCCTCCGGCACGACGG+CGG 0.598659 8.3:+26134077 None:intergenic
TATTACCGCCGTCGTGCCGG+AGG 0.600122 8.3:-26134082 MS.gene87409:CDS
GAGGATAACTCCTGGTGTGG+TGG 0.604420 8.3:-26133836 MS.gene87409:CDS
ACACAACGTGGTGGTTGTTG+GGG 0.605374 8.3:+26134929 None:intergenic
GTTGTGTTACGAACCACGAT+CGG 0.606028 8.3:-26134913 MS.gene87409:CDS
CTTTGAAATGAAACGCGGCT+CGG 0.609325 8.3:+26134385 None:intergenic
CCAAGTCGACAGAATATCGC+CGG 0.609400 8.3:+26133895 None:intergenic
GGCGTACTCCTCCGGCACGA+CGG 0.610618 8.3:+26134074 None:intergenic
AATGAGAGTTCCTCCTCCTG+CGG 0.612142 8.3:-26135039 MS.gene87409:CDS
GCTCCGGCCGAAGATAATTG+CGG 0.620112 8.3:-26133650 MS.gene87409:CDS
ACCGCGTCTTTCTTCCATGG+TGG 0.623116 8.3:-26134319 MS.gene87409:CDS
GTTGTGAAAATAGAAAACAT+TGG 0.623944 8.3:+26134243 None:intergenic
GATGTGAGGAGGATAACTCC+TGG 0.624195 8.3:-26133844 MS.gene87409:CDS
CAACGGCTACGATCACCCAC+CGG 0.625603 8.3:-26134420 MS.gene87409:CDS
CAAAGCTCGAGAACCGATCG+TGG 0.627563 8.3:+26134900 None:intergenic
TGAAACGGTGTCGTTTAAAG+CGG 0.629353 8.3:-26133959 MS.gene87409:CDS
CAACAACACCAATAGCAACT+GGG 0.629954 8.3:-26134529 MS.gene87409:CDS
CAACCTGAACACGAATTCCA+AGG 0.634144 8.3:+26134004 None:intergenic
AAAGCGGTTAGGTTCGTCGA+CGG 0.634924 8.3:-26133943 MS.gene87409:CDS
AAGAAACGCTGCCGTACCGT+CGG 0.639149 8.3:+26133870 None:intergenic
GCGAACTGACTCAACTGCAC+CGG 0.640884 8.3:+26133562 None:intergenic
TCGCTCAAGCAATATTAGAG+AGG 0.653959 8.3:-26134449 MS.gene87409:CDS
GGTTTGAACAATCTCCACCA+TGG 0.658087 8.3:+26134305 None:intergenic
CCGGAAGAAGAACGACGCCG+TGG 0.659160 8.3:-26133615 MS.gene87409:CDS
GATGATGATGAGGAAACCGC+AGG 0.662901 8.3:+26135023 None:intergenic
ACCGTTTCAAAAGTCCTCAA+CGG 0.663313 8.3:+26133973 None:intergenic
TTGTGGTGATGATGATGATG+AGG 0.674428 8.3:+26135013 None:intergenic
CCGGCCGAAGATAATTGCGG+CGG 0.681801 8.3:-26133647 MS.gene87409:CDS
ACGGCGGTAATACGAAGCAA+CGG 0.687644 8.3:+26134093 None:intergenic
GACAGCGATTCTGTTAACGC+CGG 0.688301 8.3:-26133914 MS.gene87409:CDS
GAGGAGGATAACTCCTGGTG+TGG 0.698942 8.3:-26133839 MS.gene87409:CDS
ACCATGGAAGAAAGACGCGG+TGG 0.710930 8.3:+26134321 None:intergenic
GATGATGAGGAAACCGCAGG+AGG 0.712498 8.3:+26135026 None:intergenic
TCGTGGTTCGTAACACAACG+TGG 0.724066 8.3:+26134917 None:intergenic
ATTGTTATCGAAACAATCAG+CGG 0.731325 8.3:+26134482 None:intergenic
AGCGTCTTTGAAATGAAACG+CGG 0.732740 8.3:+26134380 None:intergenic
TGGTTCGTAACACAACGTGG+TGG 0.752461 8.3:+26134920 None:intergenic
TCCACCATGGAAGAAAGACG+CGG 0.760474 8.3:+26134318 None:intergenic
GCAAATCAGAGGCTTCCACG+TGG 0.765130 8.3:-26133506 MS.gene87409:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATAGAAAACATTGGAAT+AGG + chr8.3:26134282-26134301 None:intergenic 20.0%
! ATTATGTTCTTGTTCTTGTT+GGG + chr8.3:26133683-26133702 None:intergenic 25.0%
! ATTGCTGAAAAAGCTATTAA+CGG - chr8.3:26134408-26134427 MS.gene87409:CDS 25.0%
! GTTGTGAAAATAGAAAACAT+TGG + chr8.3:26134288-26134307 None:intergenic 25.0%
! TAAACCTAAATCTTTCATGA+TGG + chr8.3:26133761-26133780 None:intergenic 25.0%
!!! GTAAATTTCTGAGGTATTAT+TGG + chr8.3:26133827-26133846 None:intergenic 25.0%
!!! TTTTTGAGAAGTGGTATTAT+GGG + chr8.3:26133802-26133821 None:intergenic 25.0%
AATAGGTGAAATTCCAGAAA+AGG + chr8.3:26134265-26134284 None:intergenic 30.0%
AGTCTTGAGTTTGATCAAAT+TGG + chr8.3:26133867-26133886 None:intergenic 30.0%
ATTGTTATCGAAACAATCAG+CGG + chr8.3:26134049-26134068 None:intergenic 30.0%
GATTATGTTCTTGTTCTTGT+TGG + chr8.3:26133684-26133703 None:intergenic 30.0%
GATTCCATCATGAAAGATTT+AGG - chr8.3:26133754-26133773 MS.gene87409:intron 30.0%
TTACTCTAATAACCAAAACC+TGG - chr8.3:26133906-26133925 MS.gene87409:CDS 30.0%
!! GTTTTTGAGAAGTGGTATTA+TGG + chr8.3:26133803-26133822 None:intergenic 30.0%
!! TTATTGGTGTTTTTGAGAAG+TGG + chr8.3:26133811-26133830 None:intergenic 30.0%
!!! CACGTTGTTGATTTTGAAAT+TGG - chr8.3:26134351-26134370 MS.gene87409:CDS 30.0%
!!! GTTGATTTTGAAATTGGTCT+TGG - chr8.3:26134357-26134376 MS.gene87409:CDS 30.0%
ACAAGAACATAATCCAGAAG+TGG - chr8.3:26133690-26133709 MS.gene87409:intron 35.0%
CCAGATTAGTGTTTGTGTTA+GGG + chr8.3:26133942-26133961 None:intergenic 35.0%
TATTAGAGAGGCTGAATCAA+CGG - chr8.3:26134091-26134110 MS.gene87409:CDS 35.0%
TCAAACCATAAGAACCTTCA+AGG - chr8.3:26134239-26134258 MS.gene87409:CDS 35.0%
TCAATACGCTTCTCTTATGA+AGG - chr8.3:26134383-26134402 MS.gene87409:CDS 35.0%
TCCAGATTAGTGTTTGTGTT+AGG + chr8.3:26133943-26133962 None:intergenic 35.0%
TGTTAGGGTAATTATAAGCC+AGG + chr8.3:26133927-26133946 None:intergenic 35.0%
! GGTAATTATAAGCCAGGTTT+TGG + chr8.3:26133921-26133940 None:intergenic 35.0%
!! GATCAAATTGGTCTTGAGAA+GGG + chr8.3:26133855-26133874 None:intergenic 35.0%
!! TATTGGTGTTGTTGTTTGGT+TGG + chr8.3:26133993-26134012 None:intergenic 35.0%
!! TGATCAAATTGGTCTTGAGA+AGG + chr8.3:26133856-26133875 None:intergenic 35.0%
!! TTGCTATTGGTGTTGTTGTT+TGG + chr8.3:26133997-26134016 None:intergenic 35.0%
AAAGGCCTTGAAGGTTCTTA+TGG + chr8.3:26134247-26134266 None:intergenic 40.0%
AATTCCAGAAAAGGCCTTGA+AGG + chr8.3:26134256-26134275 None:intergenic 40.0%
ACAACAACACCAATAGCAAC+TGG - chr8.3:26133998-26134017 MS.gene87409:CDS 40.0%
ACCGTTTCAAAAGTCCTCAA+CGG + chr8.3:26134558-26134577 None:intergenic 40.0%
ACGAATCAACCTGCTTTCAA+CGG + chr8.3:26134478-26134497 None:intergenic 40.0%
AGGGTTAGGGTAAATTTCTG+AGG + chr8.3:26133836-26133855 None:intergenic 40.0%
CAACAACACCAATAGCAACT+GGG - chr8.3:26133999-26134018 MS.gene87409:CDS 40.0%
CCCTAACACAAACACTAATC+TGG - chr8.3:26133939-26133958 MS.gene87409:CDS 40.0%
CTTCCTTGCCAAATTTGGAT+TGG - chr8.3:26133728-26133747 MS.gene87409:intron 40.0%
GAATCCCACCAATCCAAATT+TGG + chr8.3:26133739-26133758 None:intergenic 40.0%
GATGAAATCCCAGTTGCTAT+TGG + chr8.3:26134010-26134029 None:intergenic 40.0%
TAATCCAGAAGTGGAACAAG+AGG - chr8.3:26133699-26133718 MS.gene87409:intron 40.0%
TCATGCTTCCTTGCCAAATT+TGG - chr8.3:26133723-26133742 MS.gene87409:intron 40.0%
TCGCTCAAGCAATATTAGAG+AGG - chr8.3:26134079-26134098 MS.gene87409:CDS 40.0%
TGTTCTTGTTCTTGTTGGGT+TGG + chr8.3:26133679-26133698 None:intergenic 40.0%
! TTGGTTTCTTTTCTGGGCTA+GGG + chr8.3:26133660-26133679 None:intergenic 40.0%
! TTGTGGTGATGATGATGATG+AGG + chr8.3:26133518-26133537 None:intergenic 40.0%
!! AATTGGTCTTGAGAAGGGTT+AGG + chr8.3:26133850-26133869 None:intergenic 40.0%
!! AGCGTCTTTGAAATGAAACG+CGG + chr8.3:26134151-26134170 None:intergenic 40.0%
!! ATTGGTCTTGAGAAGGGTTA+GGG + chr8.3:26133849-26133868 None:intergenic 40.0%
!! CTTGCCAAATTTGGATTGGT+GGG - chr8.3:26133732-26133751 MS.gene87409:intron 40.0%
!! GTTAACGACGATGTTTTGAG+TGG + chr8.3:26133554-26133573 None:intergenic 40.0%
!! TGAAACGGTGTCGTTTAAAG+CGG - chr8.3:26134569-26134588 MS.gene87409:CDS 40.0%
!!! GTTGGGTTGGTTTCTTTTCT+GGG + chr8.3:26133666-26133685 None:intergenic 40.0%
!!! TGTTGGGTTGGTTTCTTTTC+TGG + chr8.3:26133667-26133686 None:intergenic 40.0%
AACACAACGTGGTGGTTGTT+GGG + chr8.3:26133603-26133622 None:intergenic 45.0%
CAACCTGAACACGAATTCCA+AGG + chr8.3:26134527-26134546 None:intergenic 45.0%
CCACCAATCCAAATTTGGCA+AGG + chr8.3:26133734-26133753 None:intergenic 45.0%
GCAAAGCTTCGAGTAAAGCT+TGG + chr8.3:26134314-26134333 None:intergenic 45.0%
GCTCTTCAATCTCTTCTCTC+CGG - chr8.3:26134168-26134187 MS.gene87409:CDS 45.0%
GCTTTACTCGAAGCTTTGCA+TGG - chr8.3:26134315-26134334 MS.gene87409:CDS 45.0%
GGTTTGAACAATCTCCACCA+TGG + chr8.3:26134226-26134245 None:intergenic 45.0%
GTTGTGTTACGAACCACGAT+CGG - chr8.3:26133615-26133634 MS.gene87409:CDS 45.0%
TAACACAACGTGGTGGTTGT+TGG + chr8.3:26133604-26133623 None:intergenic 45.0%
TCAACAAAAACCACCACACC+AGG + chr8.3:26134705-26134724 None:intergenic 45.0%
TGACCTTGGAATTCGTGTTC+AGG - chr8.3:26134521-26134540 MS.gene87409:CDS 45.0%
TGATCCTCTTGTTCCACTTC+TGG + chr8.3:26133706-26133725 None:intergenic 45.0%
! AGAACCTTCAAGGCCTTTTC+TGG - chr8.3:26134249-26134268 MS.gene87409:CDS 45.0%
! CCTTGCCAAATTTGGATTGG+TGG - chr8.3:26133731-26133750 MS.gene87409:intron 45.0%
! GGAGTTTTACTCGATGATGC+TGG - chr8.3:26134788-26134807 MS.gene87409:intron 45.0%
! GGATTGAGATGCTTTTGCTC+CGG - chr8.3:26134862-26134881 MS.gene87409:intron 45.0%
! GGTGTGGTGGTTTTTGTTGA+CGG - chr8.3:26134705-26134724 MS.gene87409:intron 45.0%
! GTGGTTTTTGTTGACGGTGA+GGG - chr8.3:26134711-26134730 MS.gene87409:intron 45.0%
! TTTTTGTTGACGGTGAGGGA+TGG - chr8.3:26134715-26134734 MS.gene87409:intron 45.0%
!! AACGACGATGTTTTGAGTGG+TGG + chr8.3:26133551-26133570 None:intergenic 45.0%
!! AGGTTGATTTTGTGCCGTTG+AGG - chr8.3:26134541-26134560 MS.gene87409:CDS 45.0%
!! CTTTGAAATGAAACGCGGCT+CGG + chr8.3:26134146-26134165 None:intergenic 45.0%
!! GCCGTTGAGGACTTTTGAAA+CGG - chr8.3:26134554-26134573 MS.gene87409:CDS 45.0%
!! GCGTGGTGTTGTGAATAGTT+TGG - chr8.3:26134767-26134786 MS.gene87409:intron 45.0%
!! GTTGGTTTCTTTTCTGGGCT+AGG + chr8.3:26133661-26133680 None:intergenic 45.0%
!! TCAGTTCGCGGATTTTCAAG+CGG - chr8.3:26134980-26134999 MS.gene87409:CDS 45.0%
!! TGGATTAGTCCGGTTTGAAC+CGG + chr8.3:26134190-26134209 None:intergenic 45.0%
!! TTTTGAGTGGTGGTGAGCTT+TGG + chr8.3:26133541-26133560 None:intergenic 45.0%
AAAGCGGTTAGGTTCGTCGA+CGG - chr8.3:26134585-26134604 MS.gene87409:CDS 50.0%
AATCAGTTCGCGCATGACCT+TGG - chr8.3:26134507-26134526 MS.gene87409:CDS 50.0%
ACACAACGTGGTGGTTGTTG+GGG + chr8.3:26133602-26133621 None:intergenic 50.0%
ACGGCGGTAATACGAAGCAA+CGG + chr8.3:26134438-26134457 None:intergenic 50.0%
CAGCGTTTCTTTCGGATGTG+AGG - chr8.3:26134670-26134689 MS.gene87409:intron 50.0%
CCAAGTCGACAGAATATCGC+CGG + chr8.3:26134636-26134655 None:intergenic 50.0%
CGTTTCTTTCGGATGTGAGG+AGG - chr8.3:26134673-26134692 MS.gene87409:intron 50.0%
CTCTTCTCTCCGGTTCAAAC+CGG - chr8.3:26134178-26134197 MS.gene87409:CDS 50.0%
GACAGCGATTCTGTTAACGC+CGG - chr8.3:26134614-26134633 MS.gene87409:CDS 50.0%
GATGTGAGGAGGATAACTCC+TGG - chr8.3:26134684-26134703 MS.gene87409:intron 50.0%
TCCACCATGGAAGAAAGACG+CGG + chr8.3:26134213-26134232 None:intergenic 50.0%
TCCACCGCCGCAATTATCTT+CGG + chr8.3:26134888-26134907 None:intergenic 50.0%
TCGTGGTTCGTAACACAACG+TGG + chr8.3:26133614-26133633 None:intergenic 50.0%
TGGTTCGTAACACAACGTGG+TGG + chr8.3:26133611-26133630 None:intergenic 50.0%
! CTTGCGAAGGTGCAAATCAG+AGG - chr8.3:26135011-26135030 MS.gene87409:CDS 50.0%
! GATGATGATGAGGAAACCGC+AGG + chr8.3:26133508-26133527 None:intergenic 50.0%
! GGTGGTTTTTGTTGACGGTG+AGG - chr8.3:26134710-26134729 MS.gene87409:intron 50.0%
!! AAAGACGCGGTGGATTAGTC+CGG + chr8.3:26134200-26134219 None:intergenic 50.0%
!! CGGTGTCGTTTAAAGCGGTT+AGG - chr8.3:26134574-26134593 MS.gene87409:CDS 50.0%
!! TTCTGTCGACTTGGTTCCGA+CGG - chr8.3:26134642-26134661 MS.gene87409:intron 50.0%
AACGTGGTGGTTGTTGGGGT+TGG + chr8.3:26133598-26133617 None:intergenic 55.0%
AAGAAACGCTGCCGTACCGT+CGG + chr8.3:26134661-26134680 None:intergenic 55.0%
ACCATGGAAGAAAGACGCGG+TGG + chr8.3:26134210-26134229 None:intergenic 55.0%
ACCGCGTCTTTCTTCCATGG+TGG - chr8.3:26134209-26134228 MS.gene87409:CDS 55.0%
CAAAGCTCGAGAACCGATCG+TGG + chr8.3:26133631-26133650 None:intergenic 55.0%
CCGTAAAACGCCTCTCTCCA+CGG + chr8.3:26134933-26134952 None:intergenic 55.0%
CGGTACGGCAGCGTTTCTTT+CGG - chr8.3:26134662-26134681 MS.gene87409:intron 55.0%
CTCGATGATGCTGGAGTCGT+TGG - chr8.3:26134797-26134816 MS.gene87409:intron 55.0%
CTTTCAACGGCGTACTCCTC+CGG + chr8.3:26134465-26134484 None:intergenic 55.0%
GAGGAGGATAACTCCTGGTG+TGG - chr8.3:26134689-26134708 MS.gene87409:intron 55.0%
GAGGATAACTCCTGGTGTGG+TGG - chr8.3:26134692-26134711 MS.gene87409:intron 55.0%
GATGATGAGGAAACCGCAGG+AGG + chr8.3:26133505-26133524 None:intergenic 55.0%
GCAAATCAGAGGCTTCCACG+TGG - chr8.3:26135022-26135041 MS.gene87409:CDS 55.0%
GCTCCGGCCGAAGATAATTG+CGG - chr8.3:26134878-26134897 MS.gene87409:intron 55.0%
GGTGCAGTTGAGTCAGTTCG+CGG - chr8.3:26134968-26134987 MS.gene87409:CDS 55.0%
TCCACCGCGTCTTTCTTCCA+TGG - chr8.3:26134206-26134225 MS.gene87409:CDS 55.0%
TGTTGACGGTGAGGGATGGA+CGG - chr8.3:26134719-26134738 MS.gene87409:intron 55.0%
! AGCGGAGTGTTTGCTTGCGA+AGG - chr8.3:26134998-26135017 MS.gene87409:CDS 55.0%
! AGGAGTACGCCGTTGAAAGC+AGG - chr8.3:26134466-26134485 MS.gene87409:CDS 55.0%
! CCGGCGATATTCTGTCGACT+TGG - chr8.3:26134633-26134652 MS.gene87409:intron 55.0%
! GCGAACTGACTCAACTGCAC+CGG + chr8.3:26134969-26134988 None:intergenic 55.0%
! GTGGTGGTGAGCTTTGGTTG+TGG + chr8.3:26133535-26133554 None:intergenic 55.0%
!! GTGGTTGTTGGGGTTGGTGT+CGG + chr8.3:26133592-26133611 None:intergenic 55.0%
!! TCGACTTGGTTCCGACGGTA+CGG - chr8.3:26134647-26134666 MS.gene87409:intron 55.0%
!! TGGTTGTTGGGGTTGGTGTC+GGG + chr8.3:26133591-26133610 None:intergenic 55.0%
!!! CCGTGGAGAGAGGCGTTTTA+CGG - chr8.3:26134930-26134949 MS.gene87409:CDS 55.0%
AAGAACGACGCCGTGGAGAG+AGG - chr8.3:26134920-26134939 MS.gene87409:CDS 60.0%
CAACGGCTACGATCACCCAC+CGG - chr8.3:26134108-26134127 MS.gene87409:CDS 60.0%
CCACGGCGTCGTTCTTCTTC+CGG + chr8.3:26134916-26134935 None:intergenic 60.0%
CCGCCGCAATTATCTTCGGC+CGG + chr8.3:26134884-26134903 None:intergenic 60.0%
CCGGCCGAAGATAATTGCGG+CGG - chr8.3:26134881-26134900 MS.gene87409:intron 60.0%
CGGTGTAGCGGTTTACCGGT+GGG + chr8.3:26134126-26134145 None:intergenic 60.0%
GATGAGGAAACCGCAGGAGG+AGG + chr8.3:26133502-26133521 None:intergenic 60.0%
GCCGAAGATAATTGCGGCGG+TGG - chr8.3:26134884-26134903 MS.gene87409:intron 60.0%
GCTGGAGTCGTTGGATGCCT+CGG - chr8.3:26134806-26134825 MS.gene87409:intron 60.0%
TCGGTGTAGCGGTTTACCGG+TGG + chr8.3:26134127-26134146 None:intergenic 60.0%
TCGTATTACCGCCGTCGTGC+CGG - chr8.3:26134443-26134462 MS.gene87409:CDS 60.0%
! CAACTGCACCGGCCTCATTC+CGG + chr8.3:26134958-26134977 None:intergenic 60.0%
! CAGCTTGTCGTTTCGCCACG+TGG + chr8.3:26135040-26135059 None:intergenic 60.0%
!! CGTTTTACGGCGCCGGAATG+AGG - chr8.3:26134943-26134962 MS.gene87409:CDS 60.0%
!! GGCTCGGTGTAGCGGTTTAC+CGG + chr8.3:26134130-26134149 None:intergenic 60.0%
!! GTTGGGGTTGGTGTCGGGTT+GGG + chr8.3:26133586-26133605 None:intergenic 60.0%
!! GTTGGTGTCGGGTTGGGAGT+TGG + chr8.3:26133580-26133599 None:intergenic 60.0%
!! TGAAACGCGGCTCGGTGTAG+CGG + chr8.3:26134138-26134157 None:intergenic 60.0%
!! TGTTGGGGTTGGTGTCGGGT+TGG + chr8.3:26133587-26133606 None:intergenic 60.0%
!!! AGAGAGGCGTTTTACGGCGC+CGG - chr8.3:26134936-26134955 MS.gene87409:CDS 60.0%
ATTGCGGCGGTGGAAGCTGC+CGG - chr8.3:26134894-26134913 MS.gene87409:intron 65.0%
CCGGAAGAAGAACGACGCCG+TGG - chr8.3:26134913-26134932 MS.gene87409:CDS 65.0%
GGAGTCGTTGGATGCCTCGG+TGG - chr8.3:26134809-26134828 MS.gene87409:intron 65.0%
GGCTGGAGGAGGAGGAGAGT+GGG - chr8.3:26134833-26134852 MS.gene87409:intron 65.0%
GTCGTTGGATGCCTCGGTGG+CGG - chr8.3:26134812-26134831 MS.gene87409:intron 65.0%
TATTACCGCCGTCGTGCCGG+AGG - chr8.3:26134446-26134465 MS.gene87409:CDS 65.0%
TGACGGTGAGGGATGGACGG+AGG - chr8.3:26134722-26134741 MS.gene87409:intron 65.0%
! TTACGGCGCCGGAATGAGGC+CGG - chr8.3:26134947-26134966 MS.gene87409:CDS 65.0%
ATGGACGGAGGCTGCAGCGG+CGG - chr8.3:26134734-26134753 MS.gene87409:intron 70.0%
CGGCTGGAGGAGGAGGAGAG+TGG - chr8.3:26134832-26134851 MS.gene87409:intron 70.0%
CTGCAGCGGCGGCGTCGTTT+CGG - chr8.3:26134745-26134764 MS.gene87409:intron 70.0%
GAGGAGGAGAGTGGGCGAGG+AGG - chr8.3:26134841-26134860 MS.gene87409:intron 70.0%
GAGGAGGAGGAGAGTGGGCG+AGG - chr8.3:26134838-26134857 MS.gene87409:intron 70.0%
GGCGTACTCCTCCGGCACGA+CGG + chr8.3:26134457-26134476 None:intergenic 70.0%
GGGATGGACGGAGGCTGCAG+CGG - chr8.3:26134731-26134750 MS.gene87409:intron 70.0%
GTACTCCTCCGGCACGACGG+CGG + chr8.3:26134454-26134473 None:intergenic 70.0%
! TTGGATGCCTCGGTGGCGGC+TGG - chr8.3:26134816-26134835 MS.gene87409:intron 70.0%
TCCTCCTCCAGCCGCCACCG+AGG + chr8.3:26134826-26134845 None:intergenic 75.0%
! GATGCCTCGGTGGCGGCTGG+AGG - chr8.3:26134819-26134838 MS.gene87409:intron 75.0%
! TCGGTGGCGGCTGGAGGAGG+AGG - chr8.3:26134825-26134844 MS.gene87409:intron 75.0%
! GCGGCGGCGTCGTTTCGGCG+TGG - chr8.3:26134750-26134769 MS.gene87409:intron 80.0%
!! GCCTCGGTGGCGGCTGGAGG+AGG - chr8.3:26134822-26134841 MS.gene87409:intron 80.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 26133490 26135060 26133490 ID=MS.gene87409
chr8.3 mRNA 26133490 26135060 26133490 ID=MS.gene87409.t1;Parent=MS.gene87409
chr8.3 exon 26134898 26135060 26134898 ID=MS.gene87409.t1.exon1;Parent=MS.gene87409.t1
chr8.3 CDS 26134898 26135060 26134898 ID=cds.MS.gene87409.t1;Parent=MS.gene87409.t1
chr8.3 exon 26133824 26134648 26133824 ID=MS.gene87409.t1.exon2;Parent=MS.gene87409.t1
chr8.3 CDS 26133824 26134648 26133824 ID=cds.MS.gene87409.t1;Parent=MS.gene87409.t1
chr8.3 exon 26133490 26133680 26133490 ID=MS.gene87409.t1.exon3;Parent=MS.gene87409.t1
chr8.3 CDS 26133490 26133680 26133490 ID=cds.MS.gene87409.t1;Parent=MS.gene87409.t1
Gene Sequence

>MS.gene87409

ATGAGAGTTCCTCCTCCTGCGGTTTCCTCATCATCATCATCACCACAACCAAAGCTCACCACCACTCAAAACATCGTCGTTAACGTTCCAACTCCCAACCCGACACCAACCCCAACAACCACCACGTTGTGTTACGAACCACGATCGGTTCTCGAGCTTTGTCGTAGCCCTAGCCCAGAAAAGAAACCAACCCAACAAGAACAAGAACATAATCCAGAAGTGGAACAAGAGGATCATGCTTCCTTGCCAAATTTGGATTGGTGGGATTCCATCATGAAAGATTTAGGTTTACAAGACGACTCTTCAACTCCCATAATACCACTTCTCAAAAACACCAATAATACCTCAGAAATTTACCCTAACCCTTCTCAAGACCAATTTGATCAAACTCAAGACTTCACTTCTCTCTCAGATATTTACTCTAATAACCAAAACCTGGCTTATAATTACCCTAACACAAACACTAATCTGGATCATTTAGTTAATGATTTCAATAATAACCAACCAAACAACAACACCAATAGCAACTGGGATTTCATCGAAGAGCTTATTCGTGCCGCTGATTGTTTCGATAACAATCATCTTCAACTCGCTCAAGCAATATTAGAGAGGCTGAATCAACGGCTACGATCACCCACCGGTAAACCGCTACACCGAGCCGCGTTTCATTTCAAAGACGCTCTTCAATCTCTTCTCTCCGGTTCAAACCGGACTAATCCACCGCGTCTTTCTTCCATGGTGGAGATTGTTCAAACCATAAGAACCTTCAAGGCCTTTTCTGGAATTTCACCTATTCCAATGTTTTCTATTTTCACAACGAACCAAGCTTTACTCGAAGCTTTGCATGGTTCGTTATACATGCACGTTGTTGATTTTGAAATTGGTCTTGGAATTCAATACGCTTCTCTTATGAAGGAGATTGCTGAAAAAGCTATTAACGGTTCGCCGTTGCTTCGTATTACCGCCGTCGTGCCGGAGGAGTACGCCGTTGAAAGCAGGTTGATTCGTGAGAATCTGAATCAGTTCGCGCATGACCTTGGAATTCGTGTTCAGGTTGATTTTGTGCCGTTGAGGACTTTTGAAACGGTGTCGTTTAAAGCGGTTAGGTTCGTCGACGGTGAGAAGACAGCGATTCTGTTAACGCCGGCGATATTCTGTCGACTTGGTTCCGACGGTACGGCAGCGTTTCTTTCGGATGTGAGGAGGATAACTCCTGGTGTGGTGGTTTTTGTTGACGGTGAGGGATGGACGGAGGCTGCAGCGGCGGCGTCGTTTCGGCGTGGTGTTGTGAATAGTTTGGAGTTTTACTCGATGATGCTGGAGTCGTTGGATGCCTCGGTGGCGGCTGGAGGAGGAGGAGAGTGGGCGAGGAGGATTGAGATGCTTTTGCTCCGGCCGAAGATAATTGCGGCGGTGGAAGCTGCCGGAAGAAGAACGACGCCGTGGAGAGAGGCGTTTTACGGCGCCGGAATGAGGCCGGTGCAGTTGAGTCAGTTCGCGGATTTTCAAGCGGAGTGTTTGCTTGCGAAGGTGCAAATCAGAGGCTTCCACGTGGCGAAACGACAAGCTGA
Protein sequence

>MS.gene87409.t1

MRVPPPAVSSSSSSPQPKLTTTQNIVVNVPTPNPTPTPTTTTLCYEPRSVLELCHIYSNNQNLAYNYPNTNTNLDHLVNDFNNNQPNNNTNSNWDFIEELIRAADCFDNNHLQLAQAILERLNQRLRSPTGKPLHRAAFHFKDALQSLLSGSNRTNPPRLSSMVEIVQTIRTFKAFSGISPIPMFSIFTTNQALLEALHGSLYMHVVDFEIGLGIQYASLMKEIAEKAINGSPLLRITAVVPEEYAVESRLIRENLNQFAHDLGIRVQVDFVPLRTFETVSFKAVRFVDGEKTAILLTPAIFCRLGSDGTAAFLSDVRRITPGVVVFVDDAFAPAEDNCGGGSCRKKNDAVERGVLRRRNEAGAVESVRGFSSGVFACEGANQRLPRGETTS