AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene87484

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene87484.t1 MTR_4g021760 85.032 314 44 2 1 313 1 312 0.0 548
MS.gene87484.t1 MTR_4g021845 86.301 219 28 1 1 219 1 217 1.73e-138 390
MS.gene87484.t1 MTR_4g021855 66.447 304 90 5 1 295 1 301 1.95e-138 394
MS.gene87484.t1 MTR_4g021790 76.446 242 55 1 1 242 1 240 3.82e-130 372
MS.gene87484.t1 MTR_5g055260 60.606 231 62 5 1 231 1 202 4.39e-87 261
MS.gene87484.t1 MTR_4g023980 79.137 139 27 1 55 193 35 171 1.90e-74 226
MS.gene87484.t1 MTR_6g045327 46.078 306 142 9 1 296 1 293 3.81e-72 228
MS.gene87484.t1 MTR_1g032570 38.082 365 159 9 10 309 11 373 2.12e-69 227
MS.gene87484.t1 MTR_3g086100 39.921 253 119 5 10 231 13 263 6.98e-57 193
MS.gene87484.t1 MTR_8g079940 39.695 262 125 6 9 239 24 283 2.99e-56 191
MS.gene87484.t1 MTR_2g034960 39.623 265 132 4 10 248 29 291 8.98e-56 191
MS.gene87484.t1 MTR_4g121020 39.245 265 132 4 10 248 31 292 1.02e-55 192
MS.gene87484.t1 MTR_4g121020 39.245 265 132 4 10 248 31 292 1.08e-55 191
MS.gene87484.t1 MTR_8g105600 40.726 248 125 3 4 231 3 248 2.57e-54 186
MS.gene87484.t1 MTR_1g013170 39.431 246 123 3 7 226 2 247 7.90e-51 175
MS.gene87484.t1 MTR_4g131600 36.032 247 137 4 9 234 19 265 1.05e-48 171
MS.gene87484.t1 MTR_7g117705 36.090 266 142 4 7 244 2 267 1.81e-48 168
MS.gene87484.t1 MTR_8g093040 44.068 236 109 7 1 231 1 218 4.26e-48 162
MS.gene87484.t1 MTR_3g106220 38.057 247 123 3 10 226 8 254 3.65e-47 168
MS.gene87484.t1 MTR_1g013180 36.992 246 129 3 7 226 153 398 5.65e-45 162
MS.gene87484.t1 MTR_1g032570 34.314 306 135 8 68 309 1 304 2.61e-44 159
MS.gene87484.t1 MTR_4g131570 33.200 250 141 4 10 233 17 266 3.19e-43 156
MS.gene87484.t1 MTR_2g450070 34.902 255 131 4 10 229 18 272 4.97e-43 157
MS.gene87484.t1 MTR_2g450070 34.902 255 131 4 10 229 18 272 5.91e-43 157
MS.gene87484.t1 MTR_4g131580 33.065 248 143 4 9 233 16 263 2.37e-42 154
MS.gene87484.t1 MTR_6g016850 44.375 160 85 2 4 161 3 160 6.55e-42 143
MS.gene87484.t1 MTR_3g102590 38.140 215 104 4 10 222 8 195 4.48e-41 143
MS.gene87484.t1 MTR_3g102600 37.156 218 99 3 10 226 8 188 1.50e-39 139
MS.gene87484.t1 MTR_3g102590 37.264 212 104 4 10 219 8 192 1.21e-38 135
MS.gene87484.t1 MTR_3g086100 39.175 194 86 4 68 231 1 192 1.50e-38 142
MS.gene87484.t1 MTR_3g102600 35.616 219 101 4 4 221 4 183 4.99e-37 131
MS.gene87484.t1 MTR_0450s0040 29.719 249 154 4 12 248 6 245 4.42e-32 122
MS.gene87484.t1 MTR_3g102600 36.416 173 73 2 54 226 4 139 4.99e-32 117
MS.gene87484.t1 MTR_7g026400 33.175 211 120 4 52 248 30 233 8.56e-32 125
MS.gene87484.t1 MTR_3g102600 34.524 168 73 2 54 221 4 134 2.00e-29 109
MS.gene87484.t1 MTR_4g121020 37.097 186 89 3 88 248 6 188 2.33e-29 117
MS.gene87484.t1 MTR_8g032710 23.810 357 212 10 12 309 19 374 5.35e-21 93.2
MS.gene87484.t1 MTR_1g013160 34.459 148 72 2 71 193 1 148 1.82e-19 84.0
MS.gene87484.t1 MTR_2g090675 38.298 94 55 2 135 226 125 217 4.08e-14 72.8
MS.gene87484.t1 MTR_5g041350 55.556 54 23 1 170 222 15 68 1.35e-13 70.9
MS.gene87484.t1 MTR_6g032990 44.872 78 42 1 170 246 42 119 3.94e-13 69.7
MS.gene87484.t1 MTR_7g098250 43.678 87 44 3 161 245 253 336 4.72e-13 69.7
MS.gene87484.t1 MTR_4g078810 48.276 58 30 0 169 226 157 214 6.76e-13 69.3
MS.gene87484.t1 MTR_7g088070 40.659 91 51 2 168 257 48 136 8.90e-13 67.8
MS.gene87484.t1 MTR_4g081710 51.852 54 25 1 170 222 17 70 1.62e-12 67.8
MS.gene87484.t1 MTR_2g086450 53.704 54 24 1 170 222 45 98 2.60e-12 67.4
MS.gene87484.t1 MTR_7g088070 51.786 56 26 1 168 222 48 103 2.98e-12 66.2
MS.gene87484.t1 MTR_5g014040 36.250 80 51 0 169 248 310 389 4.29e-12 67.0
MS.gene87484.t1 MTR_7g115530 47.222 72 36 2 170 239 218 289 5.42e-12 65.9
MS.gene87484.t1 MTR_7g115530 40.659 91 51 3 170 258 218 307 7.72e-12 65.5
MS.gene87484.t1 MTR_7g115530 50.000 64 30 2 170 231 218 281 1.20e-11 65.1
MS.gene87484.t1 MTR_2g027860 55.556 54 23 1 170 222 49 102 1.40e-11 64.7
MS.gene87484.t1 MTR_7g115530 40.659 91 51 3 170 258 218 307 1.71e-11 65.1
MS.gene87484.t1 MTR_7g089010 48.276 58 30 0 169 226 90 147 1.88e-11 64.3
MS.gene87484.t1 MTR_4g098870 48.148 54 28 0 169 222 309 362 1.93e-11 65.1
MS.gene87484.t1 MTR_4g098870 48.148 54 28 0 169 222 309 362 2.01e-11 65.1
MS.gene87484.t1 MTR_4g098870 48.148 54 28 0 169 222 329 382 2.13e-11 65.1
MS.gene87484.t1 MTR_4g098870 48.148 54 28 0 169 222 329 382 2.24e-11 64.7
MS.gene87484.t1 MTR_4g098870 48.148 54 28 0 169 222 348 401 2.30e-11 64.7
MS.gene87484.t1 MTR_1g080330 42.857 84 45 3 161 242 165 247 2.88e-11 63.5
MS.gene87484.t1 MTR_1g093080 45.122 82 35 3 151 229 199 273 3.36e-11 63.9
MS.gene87484.t1 MTR_7g093030 42.466 73 40 2 167 238 209 280 3.69e-11 63.2
MS.gene87484.t1 MTR_4g064730 46.552 58 31 0 169 226 130 187 4.09e-11 63.2
MS.gene87484.t1 MTR_1g080330 47.059 68 34 2 166 231 171 238 6.82e-11 63.2
MS.gene87484.t1 MTR_7g093030 47.368 57 29 1 167 222 209 265 8.56e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene87484.t1 AT2G01760 39.163 263 119 6 7 232 4 262 2.32e-56 187
MS.gene87484.t1 AT1G67710 40.449 267 123 8 11 243 8 272 3.28e-53 182
MS.gene87484.t1 AT3G16857 36.039 308 139 9 10 259 33 340 5.79e-51 179
MS.gene87484.t1 AT3G16857 36.039 308 139 9 10 259 33 340 6.44e-51 179
MS.gene87484.t1 AT2G01760 35.395 291 119 7 7 232 4 290 1.71e-50 172
MS.gene87484.t1 AT4G31920 36.910 233 137 2 10 232 13 245 3.59e-46 164
MS.gene87484.t1 AT2G25180 38.750 240 123 6 10 226 13 251 4.15e-44 159
MS.gene87484.t1 AT3G16857 34.944 269 117 9 49 259 2 270 1.11e-39 147
MS.gene87484.t1 AT5G58080 36.863 255 125 7 10 236 14 260 3.05e-39 146
MS.gene87484.t1 AT2G01760 36.816 201 87 5 68 232 1 197 6.59e-36 132
MS.gene87484.t1 AT1G67710 40.670 209 89 7 68 243 1 207 9.03e-36 134
MS.gene87484.t1 AT5G49240 30.932 236 138 5 9 219 37 272 1.22e-33 125
MS.gene87484.t1 AT3G62670 30.258 271 159 9 28 272 53 319 1.23e-31 122
MS.gene87484.t1 AT1G49190 28.125 256 153 6 10 234 30 285 6.01e-29 115
MS.gene87484.t1 AT1G67710 39.153 189 80 7 88 243 12 198 8.52e-27 109
MS.gene87484.t1 AT5G07210 27.407 270 142 7 14 231 16 283 2.29e-24 103
MS.gene87484.t1 AT5G07210 26.619 278 142 8 14 231 16 291 9.64e-22 95.9
MS.gene87484.t1 AT2G27070 24.242 264 143 7 14 222 16 277 7.73e-19 87.4
MS.gene87484.t1 AT1G49190 32.258 124 83 1 10 132 30 153 1.51e-17 83.6
MS.gene87484.t1 AT5G29000 40.566 106 54 4 132 235 200 298 8.30e-16 77.0
MS.gene87484.t1 AT5G29000 38.793 116 62 4 132 245 200 308 1.02e-15 77.4
MS.gene87484.t1 AT5G29000 38.793 116 62 4 132 245 157 265 1.15e-15 77.0
MS.gene87484.t1 AT5G29000 38.793 116 62 4 132 245 157 265 1.15e-15 77.0
MS.gene87484.t1 AT5G44190 48.333 60 31 0 167 226 144 203 1.40e-13 71.2
MS.gene87484.t1 AT5G44190 48.333 60 31 0 167 226 144 203 1.57e-13 70.9
MS.gene87484.t1 AT3G04030 55.556 54 23 1 170 222 45 98 4.00e-13 69.7
MS.gene87484.t1 AT3G04030 55.556 54 23 1 170 222 45 98 4.53e-13 69.7
MS.gene87484.t1 AT4G28610 47.126 87 40 4 170 252 225 309 8.11e-13 68.9
MS.gene87484.t1 AT5G18240 55.556 54 23 1 170 222 45 98 8.47e-13 68.9
MS.gene87484.t1 AT5G18240 55.556 54 23 1 170 222 45 98 8.47e-13 68.9
MS.gene87484.t1 AT5G18240 55.556 54 23 1 170 222 45 98 9.21e-13 68.6
MS.gene87484.t1 AT5G18240 55.556 54 23 1 170 222 45 98 9.21e-13 68.6
MS.gene87484.t1 AT5G18240 55.556 54 23 1 170 222 45 98 9.58e-13 68.6
MS.gene87484.t1 AT2G01060 45.783 83 42 2 159 238 4 86 1.17e-12 67.8
MS.gene87484.t1 AT5G59570 42.105 76 41 1 151 226 125 197 1.68e-12 67.4
MS.gene87484.t1 AT5G59570 42.105 76 41 1 151 226 125 197 1.68e-12 67.4
MS.gene87484.t1 AT3G04450 48.276 58 29 1 166 222 234 291 1.74e-12 67.4
MS.gene87484.t1 AT3G04030 52.830 53 25 0 170 222 45 97 2.61e-12 67.4
MS.gene87484.t1 AT3G04450 48.276 58 29 1 166 222 234 291 3.61e-12 67.0
MS.gene87484.t1 AT3G46640 43.210 81 43 2 146 226 123 200 3.62e-12 66.6
MS.gene87484.t1 AT3G46640 43.210 81 43 2 146 226 123 200 3.62e-12 66.6
MS.gene87484.t1 AT3G46640 43.210 81 43 2 146 226 123 200 3.74e-12 66.6
MS.gene87484.t1 AT3G04450 48.276 58 29 1 166 222 194 251 4.06e-12 66.6
MS.gene87484.t1 AT3G04450 48.276 58 29 1 166 222 194 251 4.06e-12 66.6
MS.gene87484.t1 AT1G69580 51.852 54 25 1 170 222 34 87 6.75e-12 65.9
MS.gene87484.t1 AT5G05090 42.697 89 39 3 138 226 61 137 6.92e-12 65.1
MS.gene87484.t1 AT1G69580 51.852 54 25 1 170 222 34 87 7.05e-12 65.9
MS.gene87484.t1 AT2G20570 46.552 58 31 0 169 226 152 209 7.30e-12 66.2
MS.gene87484.t1 AT2G40970 46.552 58 31 0 169 226 104 161 1.15e-11 64.3
MS.gene87484.t1 AT3G10760 48.276 58 30 0 169 226 104 161 1.41e-11 64.7
MS.gene87484.t1 AT4G18020 36.842 76 45 1 152 227 281 353 1.81e-11 65.1
MS.gene87484.t1 AT4G18020 36.842 76 45 1 152 227 281 353 1.81e-11 65.1
MS.gene87484.t1 AT4G18020 36.842 76 45 1 152 227 281 353 1.81e-11 65.1
MS.gene87484.t1 AT4G18020 36.842 76 45 1 152 227 281 353 1.81e-11 65.1
MS.gene87484.t1 AT4G18020 36.842 76 45 1 152 227 281 353 1.81e-11 65.1
MS.gene87484.t1 AT4G18020 36.842 76 45 1 152 227 281 353 1.81e-11 65.1
MS.gene87484.t1 AT4G18020 36.842 76 45 1 152 227 281 353 2.03e-11 65.1
MS.gene87484.t1 AT4G18020 36.842 76 45 1 152 227 281 353 2.03e-11 65.1
MS.gene87484.t1 AT4G18020 36.842 76 45 1 152 227 281 353 2.03e-11 65.1
MS.gene87484.t1 AT4G18020 36.842 76 45 1 152 227 281 353 2.03e-11 65.1
MS.gene87484.t1 AT4G18020 36.842 76 45 1 152 227 281 353 2.03e-11 65.1
MS.gene87484.t1 AT2G20570 47.273 55 29 0 172 226 171 225 4.92e-11 63.5
MS.gene87484.t1 AT3G24120 48.529 68 31 2 170 233 41 108 6.59e-11 62.4
MS.gene87484.t1 AT3G24120 48.529 68 31 2 170 233 41 108 6.64e-11 62.4

Find 58 sgRNAs with CRISPR-Local

Find 87 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GACATTGTGTCGCAGAATTC+AGG 0.268438 4.1:-76217886 None:intergenic
ACATATTGCCAGCCATTTAC+AGG 0.282271 4.1:+76217380 MS.gene87484:CDS
TTCAGTTACCAAATGTAGTA+TGG 0.293347 4.1:+76216655 MS.gene87484:intron
CAACGACATTGGGTGGAAAT+TGG 0.345706 4.1:+76217926 MS.gene87484:CDS
TGAGTTGTAGGGTAACATTT+TGG 0.376494 4.1:-76216772 None:intergenic
GATAAATCATCATCAACATA+TGG 0.380393 4.1:-76217998 None:intergenic
AATGTGGGGCTTGCGAATAT+TGG 0.385194 4.1:+76216968 MS.gene87484:CDS
CGAATATTGGACTAAGCCTT+TGG 0.392176 4.1:+76216981 MS.gene87484:CDS
TGACAGGAATGTTGAGTTGT+AGG 0.408636 4.1:-76216784 None:intergenic
ATCAGAAGCCATACTACATT+TGG 0.409595 4.1:-76216663 None:intergenic
AATAAAGAATATGAAATTGT+TGG 0.416766 4.1:+76217066 MS.gene87484:CDS
CTTTCTTTCCAACATGTTGA+AGG 0.419409 4.1:-76216687 None:intergenic
CAAAGAAGGCCCGTTTGTCT+TGG 0.435563 4.1:+76217157 MS.gene87484:CDS
AAAAGAAACACTTGCAAAGA+AGG 0.435703 4.1:+76217143 MS.gene87484:CDS
GGTTCAAGAAAATAGAAAAC+GGG 0.441425 4.1:+76217098 MS.gene87484:CDS
TTGCGATGCACAACGACATT+GGG 0.452490 4.1:+76217916 MS.gene87484:CDS
TTGAGACCGGCTGGGAATGA+TGG 0.453544 4.1:-76216459 None:intergenic
CTTCAACATGTTGGAAAGAA+AGG 0.459586 4.1:+76216688 MS.gene87484:CDS
AATAAATTCTTACTAATGAC+AGG 0.461511 4.1:-76216800 None:intergenic
ACATCTCCTCAATAGAGTTA+AGG 0.467190 4.1:-76216512 None:intergenic
TTCTCGTGACCAAGACAAAC+GGG 0.467708 4.1:-76217166 None:intergenic
TCAACATATGGGAACAAGTT+GGG 0.468173 4.1:-76217986 None:intergenic
TACTCACTCAATGCCGGAGC+AGG 0.473606 4.1:+76217850 MS.gene87484:CDS
GCAATAACCTTGAGACCGGC+TGG 0.475576 4.1:-76216468 None:intergenic
AAAAGTGTTGCTCATAATGT+TGG 0.490442 4.1:-76217954 None:intergenic
AAGTCATAAACATCCATGTC+TGG 0.491105 4.1:-76216746 None:intergenic
CATGTTGCAAGAATTAGAAA+AGG 0.495389 4.1:+76217033 MS.gene87484:CDS
TGGTTCAAGAAAATAGAAAA+CGG 0.501089 4.1:+76217097 MS.gene87484:CDS
AGGAAAGAATCATACTATCA+AGG 0.510494 4.1:-76217227 None:intergenic
ATATGAAATTGTTGGAAGCT+CGG 0.515274 4.1:+76217074 MS.gene87484:CDS
ATTGCGATGCACAACGACAT+TGG 0.516098 4.1:+76217915 MS.gene87484:CDS
TGTAATCGATATCATTATCA+AGG 0.517908 4.1:+76216534 MS.gene87484:CDS
GACAGGAATGTTGAGTTGTA+GGG 0.519779 4.1:-76216783 None:intergenic
ATCATGAAGTCTATTGAATG+TGG 0.526495 4.1:+76216952 MS.gene87484:CDS
ATTCTCGTGACCAAGACAAA+CGG 0.526657 4.1:-76217167 None:intergenic
AAAATGAACCTGTAAATGGC+TGG 0.527802 4.1:-76217388 None:intergenic
TTCTGATGCCTTCAACATGT+TGG 0.527887 4.1:+76216679 MS.gene87484:CDS
CACTCTCCTTAACTCTATTG+AGG 0.546467 4.1:+76216506 MS.gene87484:CDS
CTACGTGCAGTCAATCAACT+AGG 0.547867 4.1:+76217204 MS.gene87484:CDS
ATAAATCATCATCAACATAT+GGG 0.548211 4.1:-76217997 None:intergenic
AGCTCAACAGAAGAGACAAA+AGG 0.548661 4.1:+76217773 MS.gene87484:CDS
AGAAAACTCTGGTCCTGCTC+CGG 0.548712 4.1:-76217863 None:intergenic
TCATGAAGTCTATTGAATGT+GGG 0.549366 4.1:+76216953 MS.gene87484:CDS
ATCATTCCCAGCCGGTCTCA+AGG 0.552068 4.1:+76216461 MS.gene87484:CDS
ATCAACATATGGGAACAAGT+TGG 0.563419 4.1:-76217987 None:intergenic
TGAGACCGGCTGGGAATGAT+GGG 0.577892 4.1:-76216458 None:intergenic
CGATGCACAACGACATTGGG+TGG 0.580466 4.1:+76217919 MS.gene87484:CDS
AATTGCAATAACCTTGAGAC+CGG 0.581075 4.1:-76216472 None:intergenic
AGAAGCTACTCACTCAATGC+CGG 0.591913 4.1:+76217844 MS.gene87484:CDS
CAGAATTCAGGAGAAAACTC+TGG 0.602905 4.1:-76217874 None:intergenic
CATGAAGTCTATTGAATGTG+GGG 0.606058 4.1:+76216954 MS.gene87484:CDS
CAATAACCTTGAGACCGGCT+GGG 0.613963 4.1:-76216467 None:intergenic
CAACAGAAGAGACAAAAGGT+AGG 0.617016 4.1:+76217777 MS.gene87484:CDS
GACAACCCATCATTCCCAGC+CGG 0.620153 4.1:+76216453 MS.gene87484:CDS
TGATGCTCATATGCCAGACA+TGG 0.629107 4.1:+76216733 MS.gene87484:CDS
TGAAATTGTTGGAAGCTCGG+TGG 0.670504 4.1:+76217077 MS.gene87484:CDS
ATGATTTATCATCACTTGTG+TGG 0.696325 4.1:+76218011 MS.gene87484:CDS
GTTCAAGAAAATAGAAAACG+GGG 0.742093 4.1:+76217099 MS.gene87484:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAAGAATATGAAATTGT+TGG + chr4.1:76217066-76217085 MS.gene87484:CDS 15.0%
!!! AATATGTTCTTTTTTCATAT+CGG - chr4.1:76217367-76217386 None:intergenic 15.0%
!!! TGATTTTAAACTGAAATTTA+TGG + chr4.1:76217688-76217707 MS.gene87484:intron 15.0%
!! AAAAGATGAAAACTTGAATA+AGG - chr4.1:76216577-76216596 None:intergenic 20.0%
!! AATAAATTCTTACTAATGAC+AGG - chr4.1:76216803-76216822 None:intergenic 20.0%
!! AGCATAGTAAAAAAGTAATT+AGG - chr4.1:76217250-76217269 None:intergenic 20.0%
!! ATAAATCATCATCAACATAT+GGG - chr4.1:76218000-76218019 None:intergenic 20.0%
!! CAATATAAGAATAAACAAAC+AGG - chr4.1:76216630-76216649 None:intergenic 20.0%
!!! AAAAAAGATTCTGAAAATCA+TGG + chr4.1:76217335-76217354 MS.gene87484:CDS 20.0%
!!! ATATGTTCTTTTTTCATATC+GGG - chr4.1:76217366-76217385 None:intergenic 20.0%
!!! ATATTTCTTTTTTGTAGTGA+TGG + chr4.1:76216906-76216925 MS.gene87484:intron 20.0%
!!! ATGATTTTCAGAATCTTTTT+TGG - chr4.1:76217336-76217355 None:intergenic 20.0%
!!! ATGTTTTTCTCTTAATTTTC+AGG + chr4.1:76217303-76217322 MS.gene87484:intron 20.0%
!!! ATTCAAGTTTTCATCTTTTT+TGG + chr4.1:76216578-76216597 MS.gene87484:intron 20.0%
!!! TGTTTTTCTCTTAATTTTCA+GGG + chr4.1:76217304-76217323 MS.gene87484:intron 20.0%
!!! TTTTGCAGAAATATAGAAAT+CGG + chr4.1:76217744-76217763 MS.gene87484:intron 20.0%
! AGTAGTATATTATCAACAAG+AGG - chr4.1:76217657-76217676 None:intergenic 25.0%
! GATAAATCATCATCAACATA+TGG - chr4.1:76218001-76218020 None:intergenic 25.0%
! TACAAAAATGAACCTGTAAA+TGG - chr4.1:76217395-76217414 None:intergenic 25.0%
! TGGTTCAAGAAAATAGAAAA+CGG + chr4.1:76217097-76217116 MS.gene87484:CDS 25.0%
! TGTAATCGATATCATTATCA+AGG + chr4.1:76216534-76216553 MS.gene87484:CDS 25.0%
!! GTTTTTCTCTTAATTTTCAG+GGG + chr4.1:76217305-76217324 MS.gene87484:intron 25.0%
!!! AAGTCAAATTTTGTTTCAAG+AGG - chr4.1:76217824-76217843 None:intergenic 25.0%
AAAAGAAACACTTGCAAAGA+AGG + chr4.1:76217143-76217162 MS.gene87484:CDS 30.0%
AAAAGATAACTGACAAACCT+AGG - chr4.1:76217471-76217490 None:intergenic 30.0%
AGGAAAGAATCATACTATCA+AGG - chr4.1:76217230-76217249 None:intergenic 30.0%
ATATGAAATTGTTGGAAGCT+CGG + chr4.1:76217074-76217093 MS.gene87484:CDS 30.0%
ATCATGAAGTCTATTGAATG+TGG + chr4.1:76216952-76216971 MS.gene87484:CDS 30.0%
ATGATTTATCATCACTTGTG+TGG + chr4.1:76218011-76218030 MS.gene87484:CDS 30.0%
CATGTTGCAAGAATTAGAAA+AGG + chr4.1:76217033-76217052 MS.gene87484:CDS 30.0%
GGTTCAAGAAAATAGAAAAC+GGG + chr4.1:76217098-76217117 MS.gene87484:CDS 30.0%
GTTCAAGAAAATAGAAAACG+GGG + chr4.1:76217099-76217118 MS.gene87484:CDS 30.0%
TCATGAAGTCTATTGAATGT+GGG + chr4.1:76216953-76216972 MS.gene87484:CDS 30.0%
TGATTTACAACAACAGACTT+AGG - chr4.1:76217495-76217514 None:intergenic 30.0%
TTCAGTTACCAAATGTAGTA+TGG + chr4.1:76216655-76216674 MS.gene87484:intron 30.0%
TTGAATAACATGCAAGACTA+AGG - chr4.1:76217533-76217552 None:intergenic 30.0%
! CTTCATGATAGAACTTTTTG+TGG - chr4.1:76216941-76216960 None:intergenic 30.0%
!! AAAAGTGTTGCTCATAATGT+TGG - chr4.1:76217957-76217976 None:intergenic 30.0%
AAAAAGAGGTCAAGATCATG+TGG + chr4.1:76217007-76217026 MS.gene87484:CDS 35.0%
AAAATGAACCTGTAAATGGC+TGG - chr4.1:76217391-76217410 None:intergenic 35.0%
AAGTCATAAACATCCATGTC+TGG - chr4.1:76216749-76216768 None:intergenic 35.0%
AATTGCAATAACCTTGAGAC+CGG - chr4.1:76216475-76216494 None:intergenic 35.0%
ACATCTCCTCAATAGAGTTA+AGG - chr4.1:76216515-76216534 None:intergenic 35.0%
AGAGGATGTATAGAAGCTAA+TGG - chr4.1:76217639-76217658 None:intergenic 35.0%
AGTAGTGAATTATGCAGTCA+TGG + chr4.1:76217569-76217588 MS.gene87484:intron 35.0%
ATCAACATATGGGAACAAGT+TGG - chr4.1:76217990-76218009 None:intergenic 35.0%
ATCAGAAGCCATACTACATT+TGG - chr4.1:76216666-76216685 None:intergenic 35.0%
CATGAAGTCTATTGAATGTG+GGG + chr4.1:76216954-76216973 MS.gene87484:CDS 35.0%
CTTCAACATGTTGGAAAGAA+AGG + chr4.1:76216688-76216707 MS.gene87484:CDS 35.0%
CTTTCTTTCCAACATGTTGA+AGG - chr4.1:76216690-76216709 None:intergenic 35.0%
TAAGCCTTTGGTTGAAAAAG+AGG + chr4.1:76216993-76217012 MS.gene87484:CDS 35.0%
TCAACATATGGGAACAAGTT+GGG - chr4.1:76217989-76218008 None:intergenic 35.0%
! CTTTGCAAGTGTTTCTTTTG+AGG - chr4.1:76217142-76217161 None:intergenic 35.0%
! TGAGTTGTAGGGTAACATTT+TGG - chr4.1:76216775-76216794 None:intergenic 35.0%
!!! TTGACCTCTTTTTCAACCAA+AGG - chr4.1:76217000-76217019 None:intergenic 35.0%
ACATATTGCCAGCCATTTAC+AGG + chr4.1:76217380-76217399 MS.gene87484:CDS 40.0%
ACCTAAGTCTATAGTTGCCT+AGG + chr4.1:76217451-76217470 MS.gene87484:intron 40.0%
ACCTAGGCAACTATAGACTT+AGG - chr4.1:76217455-76217474 None:intergenic 40.0%
AGCTCAACAGAAGAGACAAA+AGG + chr4.1:76217773-76217792 MS.gene87484:CDS 40.0%
ATTCTCGTGACCAAGACAAA+CGG - chr4.1:76217170-76217189 None:intergenic 40.0%
CAACAGAAGAGACAAAAGGT+AGG + chr4.1:76217777-76217796 MS.gene87484:CDS 40.0%
CACTCTCCTTAACTCTATTG+AGG + chr4.1:76216506-76216525 MS.gene87484:CDS 40.0%
CAGAATTCAGGAGAAAACTC+TGG - chr4.1:76217877-76217896 None:intergenic 40.0%
CGAATATTGGACTAAGCCTT+TGG + chr4.1:76216981-76217000 MS.gene87484:CDS 40.0%
TTCTGATGCCTTCAACATGT+TGG + chr4.1:76216679-76216698 MS.gene87484:CDS 40.0%
! GACAGGAATGTTGAGTTGTA+GGG - chr4.1:76216786-76216805 None:intergenic 40.0%
! TGACAGGAATGTTGAGTTGT+AGG - chr4.1:76216787-76216806 None:intergenic 40.0%
AATGTGGGGCTTGCGAATAT+TGG + chr4.1:76216968-76216987 MS.gene87484:CDS 45.0%
AGAAGCTACTCACTCAATGC+CGG + chr4.1:76217844-76217863 MS.gene87484:CDS 45.0%
ATTGCGATGCACAACGACAT+TGG + chr4.1:76217915-76217934 MS.gene87484:CDS 45.0%
CAACGACATTGGGTGGAAAT+TGG + chr4.1:76217926-76217945 MS.gene87484:CDS 45.0%
CTACGTGCAGTCAATCAACT+AGG + chr4.1:76217204-76217223 MS.gene87484:CDS 45.0%
GACATTGTGTCGCAGAATTC+AGG - chr4.1:76217889-76217908 None:intergenic 45.0%
TGAAATTGTTGGAAGCTCGG+TGG + chr4.1:76217077-76217096 MS.gene87484:CDS 45.0%
TGATGCTCATATGCCAGACA+TGG + chr4.1:76216733-76216752 MS.gene87484:CDS 45.0%
TTCTCGTGACCAAGACAAAC+GGG - chr4.1:76217169-76217188 None:intergenic 45.0%
TTGCGATGCACAACGACATT+GGG + chr4.1:76217916-76217935 MS.gene87484:CDS 45.0%
AGAAAACTCTGGTCCTGCTC+CGG - chr4.1:76217866-76217885 None:intergenic 50.0%
CAAAGAAGGCCCGTTTGTCT+TGG + chr4.1:76217157-76217176 MS.gene87484:CDS 50.0%
CAATAACCTTGAGACCGGCT+GGG - chr4.1:76216470-76216489 None:intergenic 50.0%
ATCATTCCCAGCCGGTCTCA+AGG + chr4.1:76216461-76216480 MS.gene87484:CDS 55.0%
CGATGCACAACGACATTGGG+TGG + chr4.1:76217919-76217938 MS.gene87484:CDS 55.0%
GACAACCCATCATTCCCAGC+CGG + chr4.1:76216453-76216472 MS.gene87484:CDS 55.0%
GCAATAACCTTGAGACCGGC+TGG - chr4.1:76216471-76216490 None:intergenic 55.0%
TACTCACTCAATGCCGGAGC+AGG + chr4.1:76217850-76217869 MS.gene87484:CDS 55.0%
TGAGACCGGCTGGGAATGAT+GGG - chr4.1:76216461-76216480 None:intergenic 55.0%
TTGAGACCGGCTGGGAATGA+TGG - chr4.1:76216462-76216481 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 76216438 76218036 76216438 ID=MS.gene87484
chr4.1 mRNA 76216438 76218036 76216438 ID=MS.gene87484.t1;Parent=MS.gene87484
chr4.1 exon 76216438 76216555 76216438 ID=MS.gene87484.t1.exon1;Parent=MS.gene87484.t1
chr4.1 CDS 76216438 76216555 76216438 ID=cds.MS.gene87484.t1;Parent=MS.gene87484.t1
chr4.1 exon 76216660 76216809 76216660 ID=MS.gene87484.t1.exon2;Parent=MS.gene87484.t1
chr4.1 CDS 76216660 76216809 76216660 ID=cds.MS.gene87484.t1;Parent=MS.gene87484.t1
chr4.1 exon 76216923 76217234 76216923 ID=MS.gene87484.t1.exon3;Parent=MS.gene87484.t1
chr4.1 CDS 76216923 76217234 76216923 ID=cds.MS.gene87484.t1;Parent=MS.gene87484.t1
chr4.1 exon 76217325 76217401 76217325 ID=MS.gene87484.t1.exon4;Parent=MS.gene87484.t1
chr4.1 CDS 76217325 76217401 76217325 ID=cds.MS.gene87484.t1;Parent=MS.gene87484.t1
chr4.1 exon 76217752 76218036 76217752 ID=MS.gene87484.t1.exon5;Parent=MS.gene87484.t1
chr4.1 CDS 76217752 76218036 76217752 ID=cds.MS.gene87484.t1;Parent=MS.gene87484.t1
Gene Sequence

>MS.gene87484

ATGGCAGTTTTCACTGACAACCCATCATTCCCAGCCGGTCTCAAGGTTATTGCAATTGATCATGACATCACTCTCCTTAACTCTATTGAGGAGATGTGTAATCGATATCATTATCAAGGTTAGTTACTGAATCATACCTTATTCAAGTTTTCATCTTTTTTGGTAAAAATTCTTACTTTTATATGTATTCCTGTTTGTTTATTCTTATATTGTTTATTTCAGTTACCAAATGTAGTATGGCTTCTGATGCCTTCAACATGTTGGAAAGAAAGGACTGTTTCGATGTAATGTTGATTGATGCTCATATGCCAGACATGGATGTTTATGACTTTGTCCAAAATGTTACCCTACAACTCAACATTCCTGTCATTAGTAAGAATTTATTCAACTTTTTCATTAAAATTCATTAGTTACACATTTCACATAATTTATTCATATATATGTACTTAAGAAGTGTGAATTGACATTATATTTCTTTTTTGTAGTGATGGCTGTTGATTCCACAAAAAGTTCTATCATGAAGTCTATTGAATGTGGGGCTTGCGAATATTGGACTAAGCCTTTGGTTGAAAAAGAGGTCAAGATCATGTGGCAACATGTTGCAAGAATTAGAAAAGGTTTGATTGAAAATAAAGAATATGAAATTGTTGGAAGCTCGGTGGTTCAAGAAAATAGAAAACGGGGTAGAGAAGATGCTAATGCCTCAAAAGAAACACTTGCAAAGAAGGCCCGTTTGTCTTGGTCACGAGAATTGCATCAGCAGTTTCTACGTGCAGTCAATCAACTAGGCCTTGATAGTATGATTCTTTCCTAATTACTTTTTTACTATGCTTTGTTGCATTCTTCAACAGTTGAACTTATGTGCATGTTTTTCTCTTAATTTTCAGGGGCAACGCCAAAAAAGATTCTGAAAATCATGGATGTTCCCGATATGAAAAAAGAACATATTGCCAGCCATTTACAGGTTCATTTTTGTATTAATTTTTGTTGATTCTGTAAATTTGATATATTTTACCTAAGTCTATAGTTGCCTAGGTTTGTCAGTTATCTTTTACCTAAGTCTGTTGTTGTAAATCATTTATGTTGTTTATACCTTAGTCTTGCATGTTATTCAAACTATTGAAGTTGTAAAGTAGTGAATTATGCAGTCATGGCAGATCTTGAAGTCTGAGTTTTAGAATAGAATATAAGTATTTCACCATTAGCTTCTATACATCCTCTTGTTGATAATATACTACTTGTCTTTTGTGTGATTTTAAACTGAAATTTATGGAGTATTATTATTAACATTGTAACTGCAATGTTGTTTTGCAGAAATATAGAAATCGGTTAAAGAGCTCAACAGAAGAGACAAAAGGTAGGATGAAAAAATCAAAGACGAGTCCTCTTGAAACAAAATTTGACTTAGAAGCTACTCACTCAATGCCGGAGCAGGACCAGAGTTTTCTCCTGAATTCTGCGACACAATGTCGCGACAATTGCGATGCACAACGACATTGGGTGGAAATTGGTGAATCCAACATTATGAGCAACACTTTTTCAGACTTGCCCAACTTGTTCCCATATGTTGATGATGATTTATCATCACTTGTGTGGTGA
Protein sequence

>MS.gene87484.t1

MAVFTDNPSFPAGLKVIAIDHDITLLNSIEEMCNRYHYQVTKCSMASDAFNMLERKDCFDVMLIDAHMPDMDVYDFVQNVTLQLNIPVIMMAVDSTKSSIMKSIECGACEYWTKPLVEKEVKIMWQHVARIRKGLIENKEYEIVGSSVVQENRKRGREDANASKETLAKKARLSWSRELHQQFLRAVNQLGLDRATPKKILKIMDVPDMKKEHIASHLQKYRNRLKSSTEETKGRMKKSKTSPLETKFDLEATHSMPEQDQSFLLNSATQCRDNCDAQRHWVEIGESNIMSNTFSDLPNLFPYVDDDLSSLVW