AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene87487

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene87487.t1 MTR_4g021855 85.382 301 43 1 1 300 1 301 0.0 532
MS.gene87487.t1 MTR_4g021790 79.000 300 37 1 1 300 1 274 2.03e-173 481
MS.gene87487.t1 MTR_4g021845 84.332 217 34 0 1 217 1 217 7.19e-136 384
MS.gene87487.t1 MTR_4g021760 72.519 262 71 1 1 261 1 262 1.25e-133 381
MS.gene87487.t1 MTR_5g055260 61.654 266 75 4 1 266 1 239 3.28e-100 294
MS.gene87487.t1 MTR_4g023980 76.866 134 31 0 58 191 38 171 1.05e-73 224
MS.gene87487.t1 MTR_1g032570 45.600 250 99 4 10 225 11 257 3.69e-66 218
MS.gene87487.t1 MTR_6g045327 45.833 240 119 5 10 247 14 244 2.41e-58 192
MS.gene87487.t1 MTR_2g034960 40.698 258 126 3 10 243 29 283 2.25e-56 192
MS.gene87487.t1 MTR_4g121020 42.623 244 110 3 10 225 31 272 3.20e-56 192
MS.gene87487.t1 MTR_4g121020 42.623 244 110 3 10 225 31 272 3.22e-56 192
MS.gene87487.t1 MTR_3g086100 40.984 244 116 3 10 225 13 256 5.25e-55 187
MS.gene87487.t1 MTR_8g105600 42.213 244 121 2 10 233 9 252 5.11e-54 185
MS.gene87487.t1 MTR_8g079940 40.741 243 118 4 9 225 24 266 1.77e-53 182
MS.gene87487.t1 MTR_4g131600 39.496 238 121 4 10 224 20 257 4.07e-48 169
MS.gene87487.t1 MTR_1g013170 38.866 247 123 3 7 225 2 248 3.73e-47 165
MS.gene87487.t1 MTR_3g106220 37.849 251 118 4 10 225 8 255 4.88e-46 164
MS.gene87487.t1 MTR_7g117705 36.585 246 128 4 7 224 2 247 1.55e-44 157
MS.gene87487.t1 MTR_1g013180 37.600 250 128 3 7 228 153 402 2.15e-43 157
MS.gene87487.t1 MTR_4g131570 35.200 250 134 4 10 231 17 266 2.96e-43 156
MS.gene87487.t1 MTR_4g131580 36.032 247 133 4 10 231 17 263 6.88e-43 155
MS.gene87487.t1 MTR_1g032570 42.932 191 73 3 68 225 1 188 1.30e-42 154
MS.gene87487.t1 MTR_8g093040 40.598 234 118 6 1 229 1 218 2.73e-42 147
MS.gene87487.t1 MTR_2g450070 34.252 254 130 4 10 226 18 271 3.37e-42 154
MS.gene87487.t1 MTR_2g450070 34.252 254 130 4 10 226 18 271 4.60e-42 154
MS.gene87487.t1 MTR_6g016850 44.737 152 82 1 10 159 9 160 3.41e-40 138
MS.gene87487.t1 MTR_3g102600 36.406 217 102 2 10 225 8 189 5.55e-40 139
MS.gene87487.t1 MTR_3g086100 40.000 185 84 2 68 225 1 185 3.56e-37 138
MS.gene87487.t1 MTR_3g102590 36.449 214 109 4 10 221 8 196 4.68e-37 132
MS.gene87487.t1 MTR_3g102600 34.597 211 102 2 10 219 8 183 7.58e-36 127
MS.gene87487.t1 MTR_3g102590 35.714 210 108 4 10 217 8 192 3.37e-34 123
MS.gene87487.t1 MTR_7g026400 36.364 209 100 4 45 229 19 218 4.88e-33 128
MS.gene87487.t1 MTR_3g102600 36.207 174 76 1 52 225 2 140 1.41e-32 119
MS.gene87487.t1 MTR_0450s0040 31.330 233 145 3 12 233 6 234 1.51e-30 117
MS.gene87487.t1 MTR_4g121020 41.212 165 68 2 88 225 6 168 7.30e-30 119
MS.gene87487.t1 MTR_3g102600 33.929 168 76 1 52 219 2 134 2.37e-28 106
MS.gene87487.t1 MTR_5g041350 61.818 55 20 1 168 221 15 69 3.70e-17 80.9
MS.gene87487.t1 MTR_6g032990 63.636 55 19 1 168 221 42 96 1.20e-16 80.1
MS.gene87487.t1 MTR_1g013160 31.081 148 75 2 71 191 1 148 3.36e-16 74.7
MS.gene87487.t1 MTR_2g086450 60.000 55 21 1 168 221 45 99 1.42e-15 76.6
MS.gene87487.t1 MTR_8g032710 25.940 266 147 8 12 229 19 282 4.87e-15 75.5
MS.gene87487.t1 MTR_7g098250 46.835 79 41 1 159 236 253 331 8.38e-15 74.7
MS.gene87487.t1 MTR_4g064730 43.878 98 49 4 130 225 95 188 8.51e-15 73.9
MS.gene87487.t1 MTR_7g115530 52.055 73 32 2 168 237 218 290 1.33e-14 73.2
MS.gene87487.t1 MTR_7g115530 52.055 73 32 2 168 237 218 290 2.08e-14 72.8
MS.gene87487.t1 MTR_2g016220 48.649 74 32 2 153 220 69 142 2.44e-14 73.2
MS.gene87487.t1 MTR_2g016220 48.649 74 32 2 153 220 69 142 2.65e-14 72.8
MS.gene87487.t1 MTR_7g115530 52.055 73 32 2 168 237 218 290 3.07e-14 73.2
MS.gene87487.t1 MTR_7g089010 55.932 59 26 0 167 225 90 148 3.33e-14 72.0
MS.gene87487.t1 MTR_2g090675 45.556 90 46 3 137 224 129 217 3.99e-14 72.8
MS.gene87487.t1 MTR_4g081710 56.364 55 23 1 168 221 17 71 4.67e-14 72.0
MS.gene87487.t1 MTR_1g080330 44.828 87 45 2 164 249 171 255 7.83e-14 70.9
MS.gene87487.t1 MTR_1g080330 44.828 87 45 2 164 249 171 255 1.03e-13 71.2
MS.gene87487.t1 MTR_7g115530 53.731 67 28 2 168 233 218 282 1.07e-13 70.9
MS.gene87487.t1 MTR_7g088070 53.125 64 27 2 168 228 50 113 1.91e-13 69.3
MS.gene87487.t1 MTR_7g088070 47.368 76 37 2 168 240 50 125 2.03e-13 69.3
MS.gene87487.t1 MTR_1g093080 47.500 80 34 2 149 227 199 271 2.40e-13 70.1
MS.gene87487.t1 MTR_4g078810 55.172 58 26 0 167 224 157 214 2.65e-13 70.1
MS.gene87487.t1 MTR_2g016220 42.857 84 41 3 153 229 69 152 4.13e-13 69.3
MS.gene87487.t1 MTR_4g098870 55.357 56 25 0 167 222 329 384 5.87e-13 69.3
MS.gene87487.t1 MTR_4g098870 55.357 56 25 0 167 222 309 364 6.38e-13 69.3
MS.gene87487.t1 MTR_5g014040 43.182 88 49 1 157 244 300 386 7.47e-13 68.9
MS.gene87487.t1 MTR_1g112370 36.449 107 53 5 154 255 6 102 7.69e-13 68.2
MS.gene87487.t1 MTR_4g098870 55.357 56 25 0 167 222 309 364 8.36e-13 68.9
MS.gene87487.t1 MTR_4g098870 55.357 56 25 0 167 222 329 384 8.52e-13 68.9
MS.gene87487.t1 MTR_4g098870 55.357 56 25 0 167 222 348 403 8.90e-13 68.9
MS.gene87487.t1 MTR_0223s0040 53.571 56 25 1 166 220 16 71 9.22e-13 67.8
MS.gene87487.t1 MTR_4g113140 46.753 77 39 2 158 233 226 301 9.45e-13 68.6
MS.gene87487.t1 MTR_7g069660 51.786 56 26 1 166 220 69 124 1.05e-12 68.2
MS.gene87487.t1 MTR_2g027860 53.425 73 30 3 168 236 49 121 1.10e-12 67.8
MS.gene87487.t1 MTR_4g086835 51.667 60 29 0 166 225 272 331 1.12e-12 68.2
MS.gene87487.t1 MTR_1g053835 44.595 74 38 2 166 238 256 327 1.74e-12 67.8
MS.gene87487.t1 MTR_7g093030 47.143 70 34 2 166 234 210 277 1.96e-12 66.6
MS.gene87487.t1 MTR_3g450310 53.571 56 25 1 166 220 17 72 2.50e-12 65.5
MS.gene87487.t1 MTR_7g093030 53.571 56 25 1 166 220 210 265 2.94e-12 67.0
MS.gene87487.t1 MTR_7g115530 59.615 52 20 1 168 218 218 269 6.85e-12 65.1
MS.gene87487.t1 MTR_6g444980 35.965 114 54 4 168 280 35 130 8.12e-12 65.5
MS.gene87487.t1 MTR_2g023580 53.571 56 25 1 166 220 53 108 8.16e-12 64.3
MS.gene87487.t1 MTR_8g086410 50.000 56 27 1 166 220 107 162 9.47e-12 65.5
MS.gene87487.t1 MTR_8g086410 50.000 56 27 1 166 220 109 164 9.60e-12 65.5
MS.gene87487.t1 MTR_6g444980 35.965 114 54 4 168 280 35 130 1.04e-11 65.1
MS.gene87487.t1 MTR_5g017980 51.667 60 29 0 166 225 190 249 1.45e-11 64.3
MS.gene87487.t1 MTR_5g017980 51.667 60 29 0 166 225 191 250 1.51e-11 64.3
MS.gene87487.t1 MTR_2g084230 43.243 74 38 2 148 220 80 150 1.58e-11 64.3
MS.gene87487.t1 MTR_1g090670 36.522 115 51 4 168 281 44 137 1.94e-11 63.9
MS.gene87487.t1 MTR_2g084230 43.243 74 38 2 148 220 80 150 1.97e-11 64.3
MS.gene87487.t1 MTR_1g053800 53.968 63 28 1 162 223 171 233 2.06e-11 63.9
MS.gene87487.t1 MTR_5g027440 46.377 69 34 2 164 229 21 89 2.86e-11 63.2
MS.gene87487.t1 MTR_5g054300 32.456 114 72 2 114 226 148 257 2.98e-11 63.9
MS.gene87487.t1 MTR_1g053830 49.057 53 26 1 170 221 20 72 3.72e-11 59.3
MS.gene87487.t1 MTR_4g086835 50.820 61 29 1 166 225 272 332 3.86e-11 63.5
MS.gene87487.t1 MTR_2g027800 51.667 60 28 1 168 226 39 98 6.90e-11 62.4
MS.gene87487.t1 MTR_5g014040 40.860 93 49 2 157 244 300 391 7.72e-11 63.2
MS.gene87487.t1 MTR_2g027800 34.483 116 57 4 168 282 39 136 7.72e-11 62.4
MS.gene87487.t1 MTR_5g014040 40.860 93 49 2 157 244 260 351 9.30e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene87487.t1 AT2G01760 40.304 263 118 5 7 232 4 264 1.66e-57 189
MS.gene87487.t1 AT3G16857 40.076 262 111 4 10 225 33 294 1.22e-51 180
MS.gene87487.t1 AT3G16857 40.076 262 111 4 10 225 33 294 1.28e-51 180
MS.gene87487.t1 AT2G01760 33.146 356 148 9 7 274 4 357 1.32e-51 175
MS.gene87487.t1 AT1G67710 40.164 244 117 4 11 225 8 251 1.28e-48 169
MS.gene87487.t1 AT4G31920 37.652 247 140 5 10 243 13 258 7.69e-45 160
MS.gene87487.t1 AT2G25180 38.843 242 120 6 10 225 13 252 1.90e-43 157
MS.gene87487.t1 AT3G16857 39.013 223 90 4 49 225 2 224 1.56e-39 146
MS.gene87487.t1 AT5G58080 37.165 261 128 8 10 243 14 265 3.66e-38 142
MS.gene87487.t1 AT2G01760 38.308 201 86 4 68 232 1 199 2.43e-37 135
MS.gene87487.t1 AT3G62670 32.883 222 126 6 28 226 53 274 7.51e-33 125
MS.gene87487.t1 AT1G67710 39.785 186 84 3 68 225 1 186 1.65e-31 122
MS.gene87487.t1 AT1G49190 28.839 267 153 7 10 243 30 292 3.61e-31 120
MS.gene87487.t1 AT5G49240 31.356 236 135 3 9 217 37 272 1.79e-30 116
MS.gene87487.t1 AT5G07210 31.852 270 128 8 14 229 16 283 8.79e-29 116
MS.gene87487.t1 AT5G07210 30.935 278 128 9 14 229 16 291 2.90e-26 108
MS.gene87487.t1 AT1G67710 38.554 166 74 3 88 225 12 177 4.42e-23 99.0
MS.gene87487.t1 AT2G27070 28.378 296 149 10 14 252 16 305 8.35e-22 95.9
MS.gene87487.t1 AT1G68210 25.175 286 157 6 8 237 7 291 4.10e-21 94.0
MS.gene87487.t1 AT1G49190 33.871 124 77 2 10 130 30 151 5.20e-18 84.7
MS.gene87487.t1 AT3G04030 61.818 55 20 1 168 221 45 99 2.91e-16 79.0
MS.gene87487.t1 AT3G04030 61.818 55 20 1 168 221 45 99 3.21e-16 78.6
MS.gene87487.t1 AT2G01060 49.398 83 39 2 157 236 4 86 3.97e-16 77.4
MS.gene87487.t1 AT5G29000 38.938 113 61 3 134 245 161 266 4.48e-16 78.2
MS.gene87487.t1 AT5G29000 38.938 113 61 3 134 245 161 266 4.48e-16 78.2
MS.gene87487.t1 AT5G29000 38.938 113 61 3 134 245 204 309 4.95e-16 78.2
MS.gene87487.t1 AT5G18240 61.818 55 20 1 168 221 45 99 6.71e-16 77.8
MS.gene87487.t1 AT5G18240 61.818 55 20 1 168 221 45 99 6.71e-16 77.8
MS.gene87487.t1 AT5G18240 61.818 55 20 1 168 221 45 99 7.44e-16 77.8
MS.gene87487.t1 AT5G18240 61.818 55 20 1 168 221 45 99 7.58e-16 77.8
MS.gene87487.t1 AT5G18240 61.818 55 20 1 168 221 45 99 7.58e-16 77.8
MS.gene87487.t1 AT5G29000 37.607 117 65 3 120 235 190 299 8.54e-16 76.6
MS.gene87487.t1 AT5G59570 50.649 77 35 2 149 225 125 198 3.44e-15 74.7
MS.gene87487.t1 AT5G59570 50.649 77 35 2 149 225 125 198 3.44e-15 74.7
MS.gene87487.t1 AT3G04030 57.407 54 23 0 168 221 45 98 3.79e-15 75.5
MS.gene87487.t1 AT3G46640 49.296 71 36 0 167 237 143 213 5.87e-15 74.3
MS.gene87487.t1 AT3G46640 49.296 71 36 0 167 237 143 213 6.41e-15 74.3
MS.gene87487.t1 AT3G46640 49.296 71 36 0 167 237 143 213 6.41e-15 74.3
MS.gene87487.t1 AT5G05090 55.932 59 26 0 167 225 80 138 9.65e-15 73.2
MS.gene87487.t1 AT3G10760 55.932 59 26 0 167 225 104 162 1.97e-14 73.2
MS.gene87487.t1 AT2G40970 54.237 59 27 0 167 225 104 162 3.25e-14 71.2
MS.gene87487.t1 AT3G24120 37.963 108 53 3 168 270 41 139 4.39e-14 71.6
MS.gene87487.t1 AT3G24120 37.963 108 53 3 168 270 41 139 4.74e-14 71.6
MS.gene87487.t1 AT5G44190 58.621 58 24 0 167 224 146 203 6.45e-14 72.0
MS.gene87487.t1 AT5G44190 58.621 58 24 0 167 224 146 203 7.86e-14 71.6
MS.gene87487.t1 AT3G04450 57.407 54 22 1 168 220 238 291 7.92e-14 72.0
MS.gene87487.t1 AT3G04450 57.407 54 22 1 168 220 198 251 8.24e-14 71.6
MS.gene87487.t1 AT3G04450 57.407 54 22 1 168 220 198 251 8.24e-14 71.6
MS.gene87487.t1 AT3G04450 57.407 54 22 1 168 220 238 291 1.14e-13 70.5
MS.gene87487.t1 AT4G28610 60.000 55 21 1 168 221 225 279 1.41e-13 70.9
MS.gene87487.t1 AT4G13640 39.091 110 49 5 168 270 37 135 3.55e-13 68.9
MS.gene87487.t1 AT4G13640 39.091 110 49 5 168 270 37 135 3.69e-13 68.9
MS.gene87487.t1 AT1G69580 49.231 65 32 1 168 231 34 98 3.80e-13 69.3
MS.gene87487.t1 AT1G69580 49.231 65 32 1 168 231 34 98 4.12e-13 69.3
MS.gene87487.t1 AT2G40260 40.594 101 53 3 134 233 51 145 6.22e-13 68.9
MS.gene87487.t1 AT1G25550 48.684 76 37 2 154 227 194 269 7.19e-13 68.6
MS.gene87487.t1 AT2G20570 56.897 58 25 0 167 224 152 209 9.77e-13 68.6
MS.gene87487.t1 AT1G14600 61.538 52 19 1 170 220 25 76 1.16e-12 67.0
MS.gene87487.t1 AT5G45580 36.441 118 66 3 157 272 12 122 1.98e-12 66.6
MS.gene87487.t1 AT1G79430 43.617 94 48 2 149 237 15 108 1.98e-12 67.4
MS.gene87487.t1 AT5G06800 51.471 68 30 2 168 234 192 257 2.18e-12 67.4
MS.gene87487.t1 AT5G45580 37.838 111 60 3 164 272 3 106 2.36e-12 66.2
MS.gene87487.t1 AT5G06800 58.182 55 22 1 168 221 192 246 2.71e-12 66.2
MS.gene87487.t1 AT3G13040 53.571 56 25 1 167 221 240 295 3.44e-12 67.0
MS.gene87487.t1 AT3G13040 53.571 56 25 1 167 221 240 295 3.44e-12 67.0
MS.gene87487.t1 AT3G13040 53.571 56 25 1 167 221 240 295 3.44e-12 67.0
MS.gene87487.t1 AT5G06800 58.182 55 22 1 168 221 192 246 4.44e-12 66.2
MS.gene87487.t1 AT4G04605 53.571 56 25 1 166 220 12 67 4.72e-12 64.7
MS.gene87487.t1 AT1G68670 46.429 84 43 2 145 227 193 275 6.29e-12 65.9
MS.gene87487.t1 AT2G20570 58.182 55 23 0 170 224 171 225 6.75e-12 65.9
MS.gene87487.t1 AT2G20400 59.259 54 21 1 168 220 231 284 8.63e-12 65.5
MS.gene87487.t1 AT2G20400 59.259 54 21 1 168 220 231 284 8.68e-12 65.5
MS.gene87487.t1 AT3G12730 52.727 55 25 1 168 221 23 77 1.47e-11 63.5
MS.gene87487.t1 AT2G03500 50.000 62 30 1 167 227 232 293 2.34e-11 64.3
MS.gene87487.t1 AT4G18020 50.877 57 28 0 168 224 296 352 2.69e-11 64.3
MS.gene87487.t1 AT4G18020 50.877 57 28 0 168 224 296 352 2.69e-11 64.3
MS.gene87487.t1 AT4G18020 50.877 57 28 0 168 224 296 352 2.69e-11 64.3
MS.gene87487.t1 AT4G18020 50.877 57 28 0 168 224 296 352 2.69e-11 64.3
MS.gene87487.t1 AT4G18020 50.877 57 28 0 168 224 296 352 2.69e-11 64.3
MS.gene87487.t1 AT4G18020 50.877 57 28 0 168 224 296 352 2.69e-11 64.3
MS.gene87487.t1 AT4G18020 50.877 57 28 0 168 224 296 352 2.74e-11 64.3
MS.gene87487.t1 AT4G18020 50.877 57 28 0 168 224 296 352 2.74e-11 64.3
MS.gene87487.t1 AT4G18020 50.877 57 28 0 168 224 296 352 2.74e-11 64.3
MS.gene87487.t1 AT4G18020 50.877 57 28 0 168 224 296 352 2.74e-11 64.3
MS.gene87487.t1 AT4G18020 50.877 57 28 0 168 224 296 352 2.74e-11 64.3
MS.gene87487.t1 AT2G42660 43.750 64 35 1 158 220 40 103 3.42e-11 62.8
MS.gene87487.t1 AT2G38300 48.214 56 28 1 166 220 52 107 4.44e-11 63.2
MS.gene87487.t1 AT2G02060 55.769 52 22 1 170 220 31 82 9.37e-11 61.6
MS.gene87487.t1 AT2G02060 55.769 52 22 1 170 220 31 82 9.37e-11 61.6

Find 68 sgRNAs with CRISPR-Local

Find 79 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATTGCATCAACGATTTCTA+TGG 0.226165 4.1:+76154074 MS.gene87487:CDS
CAAAATGATTCTCAAAATTA+TGG 0.243357 4.1:+76154221 MS.gene87487:CDS
ACATGTTGCTAGTCATTTAC+AGG 0.286115 4.1:+76154266 MS.gene87487:CDS
ATCCTACCTTTACAACCTTA+TGG 0.307805 4.1:+76154633 MS.gene87487:CDS
AGAAATCGTTGATGCAATTC+TGG 0.327125 4.1:-76154070 None:intergenic
CTCAAAATTATGGATGTTCC+AGG 0.335551 4.1:+76154231 MS.gene87487:CDS
AATTATGGATGTTCCAGGTT+TGG 0.345656 4.1:+76154236 MS.gene87487:CDS
ATTATGGATGTTCCAGGTTT+GGG 0.349053 4.1:+76154237 MS.gene87487:CDS
CTTGAATTTGTTGGAAAGAA+TGG 0.358508 4.1:+76153606 MS.gene87487:CDS
TTCTGATGCCTTGAATTTGT+TGG 0.373586 4.1:+76153597 MS.gene87487:CDS
AAAGTGACTAACCTTGATAA+TGG 0.378712 4.1:-76153439 None:intergenic
TACATATTGGACTAAGCCTT+TGG 0.386876 4.1:+76153874 MS.gene87487:CDS
TAGCTGAGTGGATTGATTGC+GGG 0.403037 4.1:+76154605 MS.gene87487:CDS
GGTTGAAGAAAATAGAAAAC+GGG 0.411991 4.1:+76153985 MS.gene87487:CDS
ATCAGAAGCTGTGTTACATT+TGG 0.412873 4.1:-76153581 None:intergenic
AATGTGGGGCTTGTACATAT+TGG 0.414028 4.1:+76153861 MS.gene87487:CDS
AATAAAGAATATGAAATTGT+TGG 0.416766 4.1:+76153953 MS.gene87487:CDS
CAGTTTGAAAGTTATCGTTC+TGG 0.417272 4.1:-76154549 None:intergenic
CATTCTTTCCAACAAATTCA+AGG 0.419108 4.1:-76153605 None:intergenic
AAACTGCATATTAGGCCATA+AGG 0.423301 4.1:-76154648 None:intergenic
AATGTCGTTTGCAGAAATAT+AGG 0.426550 4.1:+76154395 MS.gene87487:intron
GAGTGGATTGATTGCGGGAC+GGG 0.433574 4.1:+76154610 MS.gene87487:CDS
TGTAATCGATTCCATTATCA+AGG 0.450836 4.1:+76153428 MS.gene87487:CDS
TCAAACTGAGCTGACTGATC+AGG 0.459166 4.1:+76154564 MS.gene87487:CDS
AATAAATTCTTACTAATGAC+AGG 0.461511 4.1:-76153718 None:intergenic
ATATGAAATTGTTGGAAGCT+TGG 0.461542 4.1:+76153961 MS.gene87487:CDS
TTGAGACCAGCAGGGAATGA+TGG 0.467536 4.1:-76153353 None:intergenic
TTCTGGCGACCAAGACAAAC+GGG 0.468019 4.1:-76154053 None:intergenic
AAGTCATAAGCATCCATGTT+TGG 0.471983 4.1:-76153664 None:intergenic
CAAAGAAGGCCCGTTTGTCT+TGG 0.473177 4.1:+76154044 MS.gene87487:CDS
AAAAGAAACACATGCAAAGA+AGG 0.478150 4.1:+76154030 MS.gene87487:CDS
CACTTTCCTCAACTCTATTG+AGG 0.492388 4.1:+76153400 MS.gene87487:CDS
CAGCTAGAAAACCATCATTC+CGG 0.492887 4.1:-76154588 None:intergenic
GCAATAACCTTGAGACCAGC+AGG 0.494358 4.1:-76153362 None:intergenic
GATGTTCCAGGTTTGGGTGT+AGG 0.505057 4.1:+76154243 MS.gene87487:CDS
CTAGCTGAGTGGATTGATTG+CGG 0.505708 4.1:+76154604 MS.gene87487:CDS
TGGTTGAAGAAAATAGAAAA+CGG 0.506154 4.1:+76153984 MS.gene87487:CDS
ATTCTGGCGACCAAGACAAA+CGG 0.509916 4.1:-76154054 None:intergenic
AGGAAAGAATCATACTATCA+AGG 0.510494 4.1:-76154114 None:intergenic
TAGGATTCATTTGAAGAGAT+CGG 0.515087 4.1:+76154414 MS.gene87487:CDS
CTATGGGCTGTCAATCAACT+AGG 0.518108 4.1:+76154091 MS.gene87487:CDS
ATCATTCCCTGCTGGTCTCA+AGG 0.520371 4.1:+76153355 MS.gene87487:CDS
ACTGATCAGGACCGGAATGA+TGG 0.523712 4.1:+76154577 MS.gene87487:CDS
TTATGAAGTCTATTCAATGT+GGG 0.525603 4.1:+76153846 MS.gene87487:CDS
TCAAACTCAATCGATCGATC+AGG 0.527821 4.1:+76154525 MS.gene87487:CDS
ATTAGGCCATAAGGTTGTAA+AGG 0.531488 4.1:-76154639 None:intergenic
ACATGTCCTACACCCAAACC+TGG 0.534820 4.1:-76154249 None:intergenic
TTGACGTCTTCTTCAACCAA+AGG 0.537631 4.1:-76153890 None:intergenic
GACAACCCATCATTCCCTGC+TGG 0.539365 4.1:+76153347 MS.gene87487:CDS
TGAGACCAGCAGGGAATGAT+GGG 0.539790 4.1:-76153352 None:intergenic
TGGCAACATGTTGTCAGAAA+CGG 0.543020 4.1:+76153920 MS.gene87487:CDS
ATTGCATCAACGATTTCTAT+GGG 0.552524 4.1:+76154075 MS.gene87487:CDS
ATTATGAAGTCTATTCAATG+TGG 0.558796 4.1:+76153845 MS.gene87487:CDS
ATTGACAAGGTTCATAGTGA+TGG 0.561362 4.1:+76154499 MS.gene87487:CDS
CTGAGCTGACTGATCAGGAC+CGG 0.566450 4.1:+76154569 MS.gene87487:CDS
TGAAATTGTTGGAAGCTTGG+TGG 0.576315 4.1:+76153964 MS.gene87487:CDS
GAAAGTGATGTCATGATCAA+TGG 0.586165 4.1:-76153384 None:intergenic
TCAAGACGAGTGCATTGACA+AGG 0.590781 4.1:+76154486 MS.gene87487:CDS
GGCCATAAGGTTGTAAAGGT+AGG 0.602949 4.1:-76154635 None:intergenic
TGATGCTCATATGCCAAACA+TGG 0.604750 4.1:+76153651 MS.gene87487:CDS
TATGAAGTCTATTCAATGTG+GGG 0.614025 4.1:+76153847 MS.gene87487:CDS
ACATCTCCTCAATAGAGTTG+AGG 0.625514 4.1:-76153406 None:intergenic
TGAGTGGATTGATTGCGGGA+CGG 0.641804 4.1:+76154609 MS.gene87487:CDS
AAGAAGACGTCAAGAGCATG+TGG 0.649026 4.1:+76153900 MS.gene87487:CDS
CGGCTGATGAAACAAAACGT+GGG 0.694912 4.1:+76154434 MS.gene87487:CDS
CAATAACCTTGAGACCAGCA+GGG 0.699486 4.1:-76153361 None:intergenic
TCGGCTGATGAAACAAAACG+TGG 0.707531 4.1:+76154433 MS.gene87487:CDS
GTTGAAGAAAATAGAAAACG+GGG 0.712341 4.1:+76153986 MS.gene87487:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAAGAATATGAAATTGT+TGG + chr4.1:76153953-76153972 MS.gene87487:CDS 15.0%
!!! ATAATTTTGAGAATCATTTT+GGG - chr4.1:76154222-76154241 None:intergenic 15.0%
!! AATAAATTCTTACTAATGAC+AGG - chr4.1:76153721-76153740 None:intergenic 20.0%
!! ACATGATTCATTCATATATA+TGG + chr4.1:76153748-76153767 MS.gene87487:intron 20.0%
!! CAAAATGATTCTCAAAATTA+TGG + chr4.1:76154221-76154240 MS.gene87487:CDS 20.0%
!!! CATAATTTTGAGAATCATTT+TGG - chr4.1:76154223-76154242 None:intergenic 20.0%
!!! TTATTTCTTTTTTGTAGTGA+TGG + chr4.1:76153799-76153818 MS.gene87487:intron 20.0%
! ACAAAGCAAAGAAAAGAATT+AGG - chr4.1:76154137-76154156 None:intergenic 25.0%
! ATTATGAAGTCTATTCAATG+TGG + chr4.1:76153845-76153864 MS.gene87487:CDS 25.0%
! TGGTTGAAGAAAATAGAAAA+CGG + chr4.1:76153984-76154003 MS.gene87487:CDS 25.0%
! TTATGAAGTCTATTCAATGT+GGG + chr4.1:76153846-76153865 MS.gene87487:CDS 25.0%
AAAAGAAACACATGCAAAGA+AGG + chr4.1:76154030-76154049 MS.gene87487:CDS 30.0%
AAAGTGACTAACCTTGATAA+TGG - chr4.1:76153442-76153461 None:intergenic 30.0%
AATTGCATCAACGATTTCTA+TGG + chr4.1:76154074-76154093 MS.gene87487:CDS 30.0%
AGGAAAGAATCATACTATCA+AGG - chr4.1:76154117-76154136 None:intergenic 30.0%
ATATGAAATTGTTGGAAGCT+TGG + chr4.1:76153961-76153980 MS.gene87487:CDS 30.0%
ATTGCATCAACGATTTCTAT+GGG + chr4.1:76154075-76154094 MS.gene87487:CDS 30.0%
CATTCTTTCCAACAAATTCA+AGG - chr4.1:76153608-76153627 None:intergenic 30.0%
GGTTGAAGAAAATAGAAAAC+GGG + chr4.1:76153985-76154004 MS.gene87487:CDS 30.0%
GTTGAAGAAAATAGAAAACG+GGG + chr4.1:76153986-76154005 MS.gene87487:CDS 30.0%
TAGGATTCATTTGAAGAGAT+CGG + chr4.1:76154414-76154433 MS.gene87487:CDS 30.0%
TATGAAGTCTATTCAATGTG+GGG + chr4.1:76153847-76153866 MS.gene87487:CDS 30.0%
! AATGTCGTTTGCAGAAATAT+AGG + chr4.1:76154395-76154414 MS.gene87487:intron 30.0%
! TGTAATCGATTCCATTATCA+AGG + chr4.1:76153428-76153447 MS.gene87487:CDS 30.0%
!! CTTGAATTTGTTGGAAAGAA+TGG + chr4.1:76153606-76153625 MS.gene87487:CDS 30.0%
!! GTCATTTACAGGTTTGTTTT+CGG + chr4.1:76154277-76154296 MS.gene87487:intron 30.0%
!!! TGAGTTTTAGGGTAACATTT+TGG - chr4.1:76153693-76153712 None:intergenic 30.0%
AAGTCATAAGCATCCATGTT+TGG - chr4.1:76153667-76153686 None:intergenic 35.0%
AATTATGGATGTTCCAGGTT+TGG + chr4.1:76154236-76154255 MS.gene87487:CDS 35.0%
ACATGTTGCTAGTCATTTAC+AGG + chr4.1:76154266-76154285 MS.gene87487:CDS 35.0%
AGAAATCGTTGATGCAATTC+TGG - chr4.1:76154073-76154092 None:intergenic 35.0%
ATCAGAAGCTGTGTTACATT+TGG - chr4.1:76153584-76153603 None:intergenic 35.0%
ATCCTACCTTTACAACCTTA+TGG + chr4.1:76154633-76154652 MS.gene87487:CDS 35.0%
ATTATGGATGTTCCAGGTTT+GGG + chr4.1:76154237-76154256 MS.gene87487:CDS 35.0%
ATTGACAAGGTTCATAGTGA+TGG + chr4.1:76154499-76154518 MS.gene87487:CDS 35.0%
CAGTTTGAAAGTTATCGTTC+TGG - chr4.1:76154552-76154571 None:intergenic 35.0%
CTCAAAATTATGGATGTTCC+AGG + chr4.1:76154231-76154250 MS.gene87487:CDS 35.0%
GAAAGTGATGTCATGATCAA+TGG - chr4.1:76153387-76153406 None:intergenic 35.0%
GTGGGAAGAAAAAATCAAAG+TGG + chr4.1:76154452-76154471 MS.gene87487:CDS 35.0%
TACATATTGGACTAAGCCTT+TGG + chr4.1:76153874-76153893 MS.gene87487:CDS 35.0%
TTCTGATGCCTTGAATTTGT+TGG + chr4.1:76153597-76153616 MS.gene87487:CDS 35.0%
! CTTTGCATGTGTTTCTTTTG+AGG - chr4.1:76154029-76154048 None:intergenic 35.0%
! GACAGGAATGTTGAGTTTTA+GGG - chr4.1:76153704-76153723 None:intergenic 35.0%
! TGACAGGAATGTTGAGTTTT+AGG - chr4.1:76153705-76153724 None:intergenic 35.0%
!! TTTACAGGTTTGTTTTCGGA+AGG + chr4.1:76154281-76154300 MS.gene87487:intron 35.0%
AATGTGGGGCTTGTACATAT+TGG + chr4.1:76153861-76153880 MS.gene87487:CDS 40.0%
ACATCTCCTCAATAGAGTTG+AGG - chr4.1:76153409-76153428 None:intergenic 40.0%
CACTTTCCTCAACTCTATTG+AGG + chr4.1:76153400-76153419 MS.gene87487:CDS 40.0%
CAGCTAGAAAACCATCATTC+CGG - chr4.1:76154591-76154610 None:intergenic 40.0%
TCAAACTCAATCGATCGATC+AGG + chr4.1:76154525-76154544 MS.gene87487:CDS 40.0%
TGAAATTGTTGGAAGCTTGG+TGG + chr4.1:76153964-76153983 MS.gene87487:CDS 40.0%
TGATGCTCATATGCCAAACA+TGG + chr4.1:76153651-76153670 MS.gene87487:CDS 40.0%
TGGCAACATGTTGTCAGAAA+CGG + chr4.1:76153920-76153939 MS.gene87487:CDS 40.0%
TTGACGTCTTCTTCAACCAA+AGG - chr4.1:76153893-76153912 None:intergenic 40.0%
!!! ATGATGGTTTTCTAGCTGAG+TGG + chr4.1:76154593-76154612 MS.gene87487:CDS 40.0%
ATTCTGGCGACCAAGACAAA+CGG - chr4.1:76154057-76154076 None:intergenic 45.0%
CAATAACCTTGAGACCAGCA+GGG - chr4.1:76153364-76153383 None:intergenic 45.0%
CGGCTGATGAAACAAAACGT+GGG + chr4.1:76154434-76154453 MS.gene87487:CDS 45.0%
GGCCATAAGGTTGTAAAGGT+AGG - chr4.1:76154638-76154657 None:intergenic 45.0%
TCAAACTGAGCTGACTGATC+AGG + chr4.1:76154564-76154583 MS.gene87487:CDS 45.0%
TCGGCTGATGAAACAAAACG+TGG + chr4.1:76154433-76154452 MS.gene87487:CDS 45.0%
! AAGAAGACGTCAAGAGCATG+TGG + chr4.1:76153900-76153919 MS.gene87487:CDS 45.0%
! CTATGGGCTGTCAATCAACT+AGG + chr4.1:76154091-76154110 MS.gene87487:CDS 45.0%
! TCAAGACGAGTGCATTGACA+AGG + chr4.1:76154486-76154505 MS.gene87487:CDS 45.0%
!! CTAGCTGAGTGGATTGATTG+CGG + chr4.1:76154604-76154623 MS.gene87487:CDS 45.0%
!! TAGCTGAGTGGATTGATTGC+GGG + chr4.1:76154605-76154624 MS.gene87487:CDS 45.0%
ACATGTCCTACACCCAAACC+TGG - chr4.1:76154252-76154271 None:intergenic 50.0%
ACTGATCAGGACCGGAATGA+TGG + chr4.1:76154577-76154596 MS.gene87487:CDS 50.0%
CAAAGAAGGCCCGTTTGTCT+TGG + chr4.1:76154044-76154063 MS.gene87487:CDS 50.0%
GATGTTCCAGGTTTGGGTGT+AGG + chr4.1:76154243-76154262 MS.gene87487:CDS 50.0%
GCAATAACCTTGAGACCAGC+AGG - chr4.1:76153365-76153384 None:intergenic 50.0%
TGAGACCAGCAGGGAATGAT+GGG - chr4.1:76153355-76153374 None:intergenic 50.0%
TTCTGGCGACCAAGACAAAC+GGG - chr4.1:76154056-76154075 None:intergenic 50.0%
TTGAGACCAGCAGGGAATGA+TGG - chr4.1:76153356-76153375 None:intergenic 50.0%
! ATCATTCCCTGCTGGTCTCA+AGG + chr4.1:76153355-76153374 MS.gene87487:CDS 50.0%
!! TGAGTGGATTGATTGCGGGA+CGG + chr4.1:76154609-76154628 MS.gene87487:CDS 50.0%
CTGAGCTGACTGATCAGGAC+CGG + chr4.1:76154569-76154588 MS.gene87487:CDS 55.0%
GACAACCCATCATTCCCTGC+TGG + chr4.1:76153347-76153366 MS.gene87487:CDS 55.0%
!! GAGTGGATTGATTGCGGGAC+GGG + chr4.1:76154610-76154629 MS.gene87487:CDS 55.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 76153332 76154660 76153332 ID=MS.gene87487
chr4.1 mRNA 76153332 76154660 76153332 ID=MS.gene87487.t1;Parent=MS.gene87487
chr4.1 exon 76153332 76153449 76153332 ID=MS.gene87487.t1.exon1;Parent=MS.gene87487.t1
chr4.1 CDS 76153332 76153449 76153332 ID=cds.MS.gene87487.t1;Parent=MS.gene87487.t1
chr4.1 exon 76153578 76153727 76153578 ID=MS.gene87487.t1.exon2;Parent=MS.gene87487.t1
chr4.1 CDS 76153578 76153727 76153578 ID=cds.MS.gene87487.t1;Parent=MS.gene87487.t1
chr4.1 exon 76153816 76154121 76153816 ID=MS.gene87487.t1.exon3;Parent=MS.gene87487.t1
chr4.1 CDS 76153816 76154121 76153816 ID=cds.MS.gene87487.t1;Parent=MS.gene87487.t1
chr4.1 exon 76154211 76154287 76154211 ID=MS.gene87487.t1.exon4;Parent=MS.gene87487.t1
chr4.1 CDS 76154211 76154287 76154211 ID=cds.MS.gene87487.t1;Parent=MS.gene87487.t1
chr4.1 exon 76154409 76154660 76154409 ID=MS.gene87487.t1.exon5;Parent=MS.gene87487.t1
chr4.1 CDS 76154409 76154660 76154409 ID=cds.MS.gene87487.t1;Parent=MS.gene87487.t1
Gene Sequence

>MS.gene87487

ATGGCTGTTTTCGTTGACAACCCATCATTCCCTGCTGGTCTCAAGGTTATTGCCATTGATCATGACATCACTTTCCTCAACTCTATTGAGGAGATGTGTAATCGATTCCATTATCAAGGTTAGTCACTTTGTTAATTTCACTTTATTCAATTCTGAATTTTCTATCTATGTTCTTACTTTTATGTATTTTTATGTTTGTTTGTTTGTTCATTCTTATGCATATTTTTATATGTTTCTTTATTTCAGTTACCAAATGTAACACAGCTTCTGATGCCTTGAATTTGTTGGAAAGAATGGACTGTTTCGATTTAATGTTGATTGATGCTCATATGCCAAACATGGATGCTTATGACTTTGTCCAAAATGTTACCCTAAAACTCAACATTCCTGTCATTAGTAAGAATTTATTCAACTTTACATGATTCATTCATATATATGGACTTAATTAGTGTGAACTGACATTATTATTATTTCTTTTTTGTAGTGATGGCTGTTGATTTCGCAAAAAGTTCTATTATGAAGTCTATTCAATGTGGGGCTTGTACATATTGGACTAAGCCTTTGGTTGAAGAAGACGTCAAGAGCATGTGGCAACATGTTGTCAGAAACGGTTCGACTGAAAATAAAGAATATGAAATTGTTGGAAGCTTGGTGGTTGAAGAAAATAGAAAACGGGGTAGAGAAGATGATAATGCCTCAAAAGAAACACATGCAAAGAAGGCCCGTTTGTCTTGGTCGCCAGAATTGCATCAACGATTTCTATGGGCTGTCAATCAACTAGGCCTTGATAGTATGATTCTTTCCTAATTCTTTTCTTTGCTTTGTTTGTATTATTTAACAACTCAACTTATGTGCATTTTTATCTCACTTATTTTTCAGAAGCAAGACCCAAAATGATTCTCAAAATTATGGATGTTCCAGGTTTGGGTGTAGGACATGTTGCTAGTCATTTACAGGTTTGTTTTCGGAAGGAGTCTAATTAAATTATTAATAATATACTACATGTATTTTGAGTGATTTTAAATTGAAACTTATAGAGTACTATTGTTAAAATTGTGATTGCAATGTCGTTTGCAGAAATATAGGATTCATTTGAAGAGATCGGCTGATGAAACAAAACGTGGGAAGAAAAAATCAAAGTGGAGTGATAGCTTTCAAGACGAGTGCATTGACAAGGTTCATAGTGATGGCTTTCAAACTCAATCGATCGATCAGGACCAGAACGATAACTTTCAAACTGAGCTGACTGATCAGGACCGGAATGATGGTTTTCTAGCTGAGTGGATTGATTGCGGGACGGGATCCTACCTTTACAACCTTATGGCCTAA
Protein sequence

>MS.gene87487.t1

MAVFVDNPSFPAGLKVIAIDHDITFLNSIEEMCNRFHYQVTKCNTASDALNLLERMDCFDLMLIDAHMPNMDAYDFVQNVTLKLNIPVIMMAVDFAKSSIMKSIQCGACTYWTKPLVEEDVKSMWQHVVRNGSTENKEYEIVGSLVVEENRKRGREDDNASKETHAKKARLSWSPELHQRFLWAVNQLGLDKARPKMILKIMDVPGLGVGHVASHLQKYRIHLKRSADETKRGKKKSKWSDSFQDECIDKVHSDGFQTQSIDQDQNDNFQTELTDQDRNDGFLAEWIDCGTGSYLYNLMA