AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene87509

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene87509.t1 MTR_4g022370 97.253 364 9 1 1 363 1 364 0.0 723
MS.gene87509.t1 MTR_7g059400 50.000 384 125 15 1 359 1 342 1.02e-87 268
MS.gene87509.t1 MTR_4g063780 41.803 366 153 14 1 342 1 330 1.01e-66 214
MS.gene87509.t1 MTR_2g096740 40.719 334 112 18 1 314 1 268 1.31e-55 184
MS.gene87509.t1 MTR_2g096740 38.782 312 122 16 1 307 1 248 2.10e-48 165
MS.gene87509.t1 MTR_7g024670 36.573 391 184 17 1 353 1 365 2.69e-46 162
MS.gene87509.t1 MTR_3g091820 63.636 88 28 1 51 138 16 99 3.61e-32 123
MS.gene87509.t1 MTR_4g088580 52.459 122 52 2 83 200 56 175 2.48e-31 122
MS.gene87509.t1 MTR_2g093220 80.328 61 12 0 76 136 34 94 4.81e-31 119
MS.gene87509.t1 MTR_8g027295 45.324 139 65 3 69 205 11 140 6.66e-31 118
MS.gene87509.t1 MTR_8g479350 51.351 111 49 2 82 188 68 177 8.63e-30 117
MS.gene87509.t1 MTR_4g461080 65.385 78 26 1 57 134 5 81 3.65e-29 114
MS.gene87509.t1 MTR_5g031440 81.818 55 10 0 78 132 39 93 3.91e-29 115
MS.gene87509.t1 MTR_8g015840 75.000 68 15 2 74 140 13 79 5.56e-29 112
MS.gene87509.t1 MTR_3g077750 63.855 83 27 2 50 132 32 111 6.24e-29 115
MS.gene87509.t1 MTR_3g090430 64.865 74 26 0 63 136 32 105 7.06e-29 114
MS.gene87509.t1 MTR_8g068210 69.841 63 19 0 79 141 29 91 2.25e-28 113
MS.gene87509.t1 MTR_8g079060 54.255 94 32 2 61 153 17 100 2.38e-28 110
MS.gene87509.t1 MTR_2g014170 86.275 51 7 0 81 131 44 94 3.11e-28 112
MS.gene87509.t1 MTR_1g056810 56.250 96 36 1 84 179 14 103 2.78e-27 109
MS.gene87509.t1 MTR_2g059540 86.000 50 7 0 81 130 30 79 4.99e-27 106
MS.gene87509.t1 MTR_1g077600 55.000 80 34 1 59 136 18 97 1.00e-26 107
MS.gene87509.t1 MTR_1g077600 55.000 80 34 1 59 136 18 97 1.13e-26 107
MS.gene87509.t1 MTR_4g109980 80.769 52 10 0 81 132 39 90 1.32e-26 108
MS.gene87509.t1 MTR_2g014060 66.667 72 19 2 62 133 10 76 3.78e-26 107
MS.gene87509.t1 MTR_4g089095 74.138 58 14 1 82 139 50 106 6.83e-26 105
MS.gene87509.t1 MTR_4g089095 74.138 58 14 1 82 139 16 72 8.22e-26 104
MS.gene87509.t1 MTR_8g044220 68.333 60 19 0 78 137 114 173 8.35e-26 107
MS.gene87509.t1 MTR_6g012450 67.213 61 20 0 78 138 132 192 2.39e-25 107
MS.gene87509.t1 MTR_2g013370 71.930 57 15 1 80 136 44 99 2.66e-25 103
MS.gene87509.t1 MTR_2g016030 70.175 57 17 0 79 135 44 100 6.35e-25 99.8
MS.gene87509.t1 MTR_6g027450 62.903 62 23 0 75 136 82 143 8.32e-25 103
MS.gene87509.t1 MTR_7g010950 62.500 64 24 0 75 138 128 191 1.32e-24 105
MS.gene87509.t1 MTR_4g082060 65.079 63 22 0 78 140 118 180 1.40e-24 104
MS.gene87509.t1 MTR_5g041420 45.833 96 49 1 43 138 102 194 2.79e-24 102
MS.gene87509.t1 MTR_7g086780 62.295 61 23 0 75 135 92 152 4.05e-24 102
MS.gene87509.t1 MTR_3g435480 63.492 63 23 0 78 140 132 194 4.58e-24 103
MS.gene87509.t1 MTR_6g027460 60.656 61 24 0 75 135 89 149 7.34e-24 101
MS.gene87509.t1 MTR_5g041380 53.247 77 36 0 66 142 111 187 1.11e-22 98.2
MS.gene87509.t1 MTR_5g041400 48.235 85 44 0 58 142 95 179 4.81e-22 96.3
MS.gene87509.t1 MTR_5g041530 45.977 87 44 1 52 135 107 193 7.23e-22 95.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene87509.t1 AT3G55370 38.243 387 168 17 1 350 1 353 8.24e-56 186
MS.gene87509.t1 AT3G55370 43.599 289 115 13 1 275 1 255 1.47e-55 185
MS.gene87509.t1 AT2G37590 41.337 329 148 11 1 308 1 305 5.22e-50 171
MS.gene87509.t1 AT5G02460 39.362 376 140 13 1 312 1 352 1.08e-48 169
MS.gene87509.t1 AT3G55370 47.059 238 93 12 45 275 89 300 1.26e-48 168
MS.gene87509.t1 AT2G28810 34.688 369 176 15 1 342 1 331 3.11e-38 140
MS.gene87509.t1 AT1G07640 50.694 144 65 3 5 142 2 145 6.07e-37 136
MS.gene87509.t1 AT1G07640 53.731 134 56 3 15 142 4 137 1.69e-36 135
MS.gene87509.t1 AT5G60200 57.407 108 45 1 78 184 51 158 1.79e-36 133
MS.gene87509.t1 AT1G07640 70.886 79 23 0 64 142 3 81 3.63e-36 132
MS.gene87509.t1 AT2G28810 34.897 341 164 12 23 342 11 314 5.67e-36 133
MS.gene87509.t1 AT3G45610 55.238 105 44 2 78 181 36 138 5.12e-34 126
MS.gene87509.t1 AT5G65590 38.983 236 100 9 42 246 8 230 4.23e-32 123
MS.gene87509.t1 AT1G51700 60.000 95 34 3 77 167 27 121 9.61e-31 116
MS.gene87509.t1 AT1G28310 79.310 58 12 0 82 139 27 84 3.46e-30 117
MS.gene87509.t1 AT2G28510 81.967 61 11 0 81 141 47 107 4.27e-30 117
MS.gene87509.t1 AT1G28310 79.310 58 12 0 82 139 51 108 5.38e-30 117
MS.gene87509.t1 AT5G60850 71.014 69 20 0 69 137 38 106 2.00e-29 115
MS.gene87509.t1 AT3G61850 75.410 61 15 0 78 138 58 118 6.21e-29 114
MS.gene87509.t1 AT3G61850 75.410 61 15 0 78 138 58 118 6.21e-29 114
MS.gene87509.t1 AT3G61850 75.410 61 15 0 78 138 70 130 8.25e-29 114
MS.gene87509.t1 AT3G21270 84.906 53 8 0 82 134 29 81 9.21e-29 111
MS.gene87509.t1 AT3G61850 75.410 61 15 0 78 138 58 118 9.94e-29 114
MS.gene87509.t1 AT3G61850 75.410 61 15 0 78 138 70 130 1.29e-28 114
MS.gene87509.t1 AT4G38000 73.438 64 16 1 76 138 35 98 4.41e-28 110
MS.gene87509.t1 AT3G52440 58.140 86 36 0 79 164 22 107 4.70e-28 110
MS.gene87509.t1 AT4G24060 73.770 61 16 0 76 136 47 107 6.70e-28 112
MS.gene87509.t1 AT3G52440 58.140 86 36 0 79 164 41 126 7.81e-28 110
MS.gene87509.t1 AT5G66940 48.958 96 38 2 63 147 2 97 4.93e-27 107
MS.gene87509.t1 AT1G21340 68.750 64 20 0 80 143 36 99 7.84e-27 107
MS.gene87509.t1 AT5G62940 86.000 50 7 0 83 132 74 123 1.91e-26 108
MS.gene87509.t1 AT2G34140 51.685 89 40 1 47 135 26 111 2.36e-26 103
MS.gene87509.t1 AT3G50410 69.492 59 18 0 82 140 30 88 3.29e-26 105
MS.gene87509.t1 AT4G00940 71.186 59 17 0 78 136 64 122 5.16e-26 106
MS.gene87509.t1 AT4G00940 71.186 59 17 0 78 136 64 122 5.16e-26 106
MS.gene87509.t1 AT4G00940 71.186 59 17 0 78 136 64 122 5.16e-26 106
MS.gene87509.t1 AT2G46590 78.846 52 11 0 78 129 64 115 2.11e-25 105
MS.gene87509.t1 AT2G46590 78.846 52 11 0 78 129 76 127 2.41e-25 105
MS.gene87509.t1 AT4G21050 66.129 62 21 0 75 136 17 78 5.73e-25 101
MS.gene87509.t1 AT1G29160 68.966 58 18 0 78 135 58 115 5.84e-25 100
MS.gene87509.t1 AT3G47500 64.062 64 23 0 75 138 103 166 1.13e-24 104
MS.gene87509.t1 AT1G64620 74.545 55 14 0 76 130 43 97 1.39e-24 103
MS.gene87509.t1 AT1G26790 55.072 69 31 0 67 135 96 164 3.53e-24 102
MS.gene87509.t1 AT1G69570 62.295 61 23 0 75 135 125 185 5.51e-24 102
MS.gene87509.t1 AT5G62430 58.209 67 28 0 75 141 47 113 1.29e-23 99.8
MS.gene87509.t1 AT5G39660 62.903 62 23 0 74 135 130 191 1.37e-23 101
MS.gene87509.t1 AT5G39660 62.903 62 23 0 74 135 130 191 1.37e-23 101
MS.gene87509.t1 AT5G39660 62.903 62 23 0 74 135 130 191 1.37e-23 101
MS.gene87509.t1 AT1G47655 68.333 60 17 1 73 130 18 77 1.93e-23 97.1
MS.gene87509.t1 AT4G21040 50.562 89 40 1 84 172 27 111 4.84e-22 94.0
MS.gene87509.t1 AT4G21080 47.674 86 41 1 84 169 27 108 8.62e-21 90.9
MS.gene87509.t1 AT4G21030 55.932 59 23 1 71 129 13 68 2.88e-14 71.2

Find 100 sgRNAs with CRISPR-Local

Find 126 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCAAGGAAGTGAACAATTT+TGG 0.154411 4.1:-75970527 MS.gene87509:CDS
GATTTCGTCGGAGGAGATTT+TGG 0.157078 4.1:-75970894 MS.gene87509:CDS
AATTGGACTGATCATGTTTC+TGG 0.157222 4.1:-75970471 MS.gene87509:CDS
TCAACTTGCTTCTGTGAAAA+TGG 0.216358 4.1:-75970604 MS.gene87509:CDS
TGGAATAGTGCTGTTATTAC+TGG 0.251462 4.1:-75970507 MS.gene87509:CDS
CCAGAAGTTGAAATCACTTT+TGG 0.251474 4.1:+75970636 None:intergenic
GCAAGTCTTGCAGAAGTGTC+TGG 0.259253 4.1:+75971187 None:intergenic
CACCTCAAATGTCATCATTA+AGG 0.271108 4.1:-75970956 MS.gene87509:CDS
CCAAAAGTGATTTCAACTTC+TGG 0.271253 4.1:-75970636 MS.gene87509:CDS
GGTGATTTGGGAAGTGCTTT+AGG 0.279369 4.1:-75970837 MS.gene87509:CDS
GGAAGAGTGGATATTGTTAT+TGG 0.289267 4.1:+75970998 None:intergenic
ATGGGAGGAGTAGGTGATTT+GGG 0.291520 4.1:-75970849 MS.gene87509:CDS
CTGGTAATTCTGCAGCTAAT+TGG 0.303967 4.1:-75970488 MS.gene87509:CDS
GAGACGGAGCCGCCGTTTCC+TGG 0.321013 4.1:+75971422 None:intergenic
CATGGGAGGAGTAGGTGATT+TGG 0.324852 4.1:-75970850 MS.gene87509:CDS
TCTCCTCCGACGAAATCATT+AGG 0.333438 4.1:+75970900 None:intergenic
GGACAAGAGGCGGGGCTCTA+AGG 0.342022 4.1:-75971154 MS.gene87509:CDS
TCCCGGTCGGTGGAGGCTTT+CGG 0.354829 4.1:-75971127 MS.gene87509:CDS
AATTAGCCAGAAAAGGAAAT+TGG 0.359477 4.1:+75970742 None:intergenic
CACTCTTCCGCCGATATATT+AGG 0.387275 4.1:-75970984 MS.gene87509:CDS
GCCCTATCGGACATCGATCC+TGG 0.393185 4.1:+75971314 None:intergenic
TGTGACTGTGGTGGTGGTGG+TGG 0.398669 4.1:+75971377 None:intergenic
AATGGAAGATAGTATCAATA+GGG 0.409067 4.1:-75970586 MS.gene87509:CDS
CTTCAAGTGGTTTGGAGCAA+TGG 0.413375 4.1:-75970797 MS.gene87509:CDS
TTGAACTAGCACTCCCCGTT+TGG 0.413567 4.1:+75971036 None:intergenic
CTCCTCCGACGAAATCATTA+GGG 0.413580 4.1:+75970901 None:intergenic
AAATGGAAGATAGTATCAAT+AGG 0.414206 4.1:-75970587 MS.gene87509:CDS
CTTATCTTGATGCAGCCAAC+TGG 0.417162 4.1:-75971716 MS.gene87509:CDS
GCAAGCTCTGACCGCCAAAC+GGG 0.420432 4.1:-75971050 MS.gene87509:CDS
ACACTATAAAATTCTACATC+TGG 0.437923 4.1:+75970418 None:intergenic
GGCAAGCTCTGACCGCCAAA+CGG 0.445526 4.1:-75971051 MS.gene87509:CDS
GCAAGACTTGCAGAAGGTAC+TGG 0.454376 4.1:-75971175 MS.gene87509:CDS
TGCAGCCAACTGGCAGCAAC+AGG 0.457644 4.1:-75971706 MS.gene87509:intron
GAGGATCCACCGGCTCAATT+AGG 0.467697 4.1:-75971337 MS.gene87509:CDS
GTTCGCCATCCTTGCCCTAT+CGG 0.470894 4.1:+75971301 None:intergenic
CCCTCCAAATTAGCCAGAAA+AGG 0.471049 4.1:+75970735 None:intergenic
GAAGGTACTGGACAAGAGGC+GGG 0.472489 4.1:-75971163 MS.gene87509:CDS
TTTGAACATTTCCTTCAAAA+GGG 0.473490 4.1:+75970694 None:intergenic
AGGAATGTCCCGGTCGGTGG+AGG 0.474332 4.1:-75971134 MS.gene87509:CDS
CTCCGAAAGCCTCCACCGAC+CGG 0.477068 4.1:+75971125 None:intergenic
AACTAGCACTCCCCGTTTGG+CGG 0.478302 4.1:+75971039 None:intergenic
GTTTGGCGGTCAGAGCTTGC+CGG 0.480338 4.1:+75971053 None:intergenic
AGGTGTAAGGCCTAATATAT+CGG 0.483491 4.1:+75970974 None:intergenic
GGAAACAATAACAGTAATCA+AGG 0.492146 4.1:-75970543 MS.gene87509:CDS
ATGATGACATTTGAGGTGTA+AGG 0.497793 4.1:+75970961 None:intergenic
TTTAGTTACATGGGAGGAGT+AGG 0.502079 4.1:-75970858 MS.gene87509:CDS
TGAGATTGTGACTGTGGTGG+TGG 0.503091 4.1:+75971371 None:intergenic
CAAGTCTTGCAGAAGTGTCT+GGG 0.504581 4.1:+75971188 None:intergenic
GATTGTGACTGTGGTGGTGG+TGG 0.504773 4.1:+75971374 None:intergenic
AACCTTAATGATGACATTTG+AGG 0.519434 4.1:+75970954 None:intergenic
GGACATTTCAGTGCTGCCTC+CGG 0.520025 4.1:+75971269 None:intergenic
AGAGTGGATATTGTTATTGG+AGG 0.520652 4.1:+75971001 None:intergenic
TTCAGTGCTGCCTCCGGCAT+AGG 0.523439 4.1:+75971275 None:intergenic
CTAAGGAATGTCCCGGTCGG+TGG 0.528029 4.1:-75971137 MS.gene87509:CDS
TTACTGAAGTTATGATGTAA+AGG 0.534579 4.1:+75970924 None:intergenic
ATCAAGATAAGCTGCTGGTA+TGG 0.536728 4.1:+75971728 None:intergenic
ATTTACCTGTTGCTGCCAGT+TGG 0.539661 4.1:+75971701 None:intergenic
GATGGCGAACATGCCTATGC+CGG 0.544192 4.1:-75971288 MS.gene87509:CDS
CATCATCAGCAACCAGGAAA+CGG 0.545159 4.1:-75971434 MS.gene87509:CDS
CGATCCTGGCCTAATTGAGC+CGG 0.550851 4.1:+75971328 None:intergenic
TGGAAACTCAACAAAGTCTC+CGG 0.554123 4.1:-75971072 MS.gene87509:CDS
CAAGTGGTTTGGAGCAATGG+AGG 0.557508 4.1:-75970794 MS.gene87509:CDS
CCTCATGGAGGAGGATCCAC+CGG 0.558615 4.1:-75971347 MS.gene87509:CDS
TCCGAAAGCCTCCACCGACC+GGG 0.562265 4.1:+75971126 None:intergenic
GCCAGGATCGATGTCCGATA+GGG 0.563470 4.1:-75971315 MS.gene87509:CDS
CAGTCACAATCTCAACCTCA+TGG 0.566499 4.1:-75971362 MS.gene87509:CDS
TCAAGCAAAAGTAGCAACAA+TGG 0.567762 4.1:-75971092 MS.gene87509:CDS
TCCTGGCCTAATTGAGCCGG+TGG 0.569726 4.1:+75971331 None:intergenic
CAGAATCATCATCAGCAACC+AGG 0.572861 4.1:-75971440 MS.gene87509:CDS
GATGTCCGATAGGGCAAGGA+TGG 0.573727 4.1:-75971306 MS.gene87509:CDS
AGTAACCCTAATGATTTCGT+CGG 0.581465 4.1:-75970906 MS.gene87509:CDS
CTAATATATCGGCGGAAGAG+TGG 0.581651 4.1:+75970985 None:intergenic
TCCACCGGCTCAATTAGGCC+AGG 0.583672 4.1:-75971332 MS.gene87509:CDS
GATTTGGGAAGTGCTTTAGG+AGG 0.585086 4.1:-75970834 MS.gene87509:CDS
GGCCAGGATCGATGTCCGAT+AGG 0.588624 4.1:-75971316 MS.gene87509:CDS
CAATCTCAACCTCATGGAGG+AGG 0.588847 4.1:-75971356 MS.gene87509:CDS
CGGGGCTCTAAGGAATGTCC+CGG 0.591050 4.1:-75971144 MS.gene87509:CDS
GCTCTAAGGAATGTCCCGGT+CGG 0.592207 4.1:-75971140 MS.gene87509:CDS
CTGAAGCAGTTGTTGAGAGA+CGG 0.593094 4.1:+75971406 None:intergenic
GGCGAACATGCCTATGCCGG+AGG 0.593250 4.1:-75971285 MS.gene87509:CDS
AGAAGGTACTGGACAAGAGG+CGG 0.593495 4.1:-75971164 MS.gene87509:CDS
GCTGCATCAAGATAAGCTGC+TGG 0.594629 4.1:+75971723 None:intergenic
AACCCTAATGATTTCGTCGG+AGG 0.598673 4.1:-75970903 MS.gene87509:CDS
TGCAGAAGGTACTGGACAAG+AGG 0.603431 4.1:-75971167 MS.gene87509:CDS
CATGAGATGATAACTAGTGG+TGG 0.609089 4.1:-75970669 MS.gene87509:CDS
GATCGATGTCCGATAGGGCA+AGG 0.614474 4.1:-75971310 MS.gene87509:CDS
CAAGCTCTGACCGCCAAACG+GGG 0.623809 4.1:-75971049 MS.gene87509:CDS
AATCATGAGATGATAACTAG+TGG 0.640481 4.1:-75970672 MS.gene87509:CDS
ATGGAAGATAGTATCAATAG+GGG 0.643018 4.1:-75970585 MS.gene87509:CDS
TGTTGATGAGTCATTGACAT+TGG 0.643301 4.1:+75970768 None:intergenic
TGAGGTTGAGATTGTGACTG+TGG 0.644332 4.1:+75971365 None:intergenic
GGTTGAGATTGTGACTGTGG+TGG 0.650158 4.1:+75971368 None:intergenic
CATCAGCAACCAGGAAACGG+CGG 0.659295 4.1:-75971431 MS.gene87509:CDS
ACTTCTGCAAGACTTGCAGA+AGG 0.679196 4.1:-75971181 MS.gene87509:CDS
CCGGTGGATCCTCCTCCATG+AGG 0.687789 4.1:+75971347 None:intergenic
AAGGTACTGGACAAGAGGCG+GGG 0.703126 4.1:-75971162 MS.gene87509:CDS
TGTAAGGCCTAATATATCGG+CGG 0.708601 4.1:+75970977 None:intergenic
TAACTAGTGGTGGATATGTG+AGG 0.730675 4.1:-75970659 MS.gene87509:CDS
GTGTTGGTTGATTCACAACG+TGG 0.731087 4.1:+75971248 None:intergenic
TCACAATCTCAACCTCATGG+AGG 0.766876 4.1:-75971359 MS.gene87509:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATAATTCAAAAGATAAAA+AGG - chr4.1:75970554-75970573 MS.gene87509:CDS 10.0%
!! TAAGAAAGAAATCAAATATT+TGG + chr4.1:75970627-75970646 None:intergenic 15.0%
!! AACAACATAAATAGACAAAA+AGG + chr4.1:75970702-75970721 None:intergenic 20.0%
!! AATTGAGAATAATAGAAGAA+AGG + chr4.1:75970653-75970672 None:intergenic 20.0%
!! ACAGGTAAATATAATTAGTA+AGG - chr4.1:75970484-75970503 MS.gene87509:CDS 20.0%
!! AGTAAGGTTTCAATTTAATT+TGG - chr4.1:75970500-75970519 MS.gene87509:CDS 20.0%
!!! AATTCAATTTTATCTTCAAG+TGG - chr4.1:75971362-75971381 MS.gene87509:CDS 20.0%
!!! TTTTGAATTATTTTGCCTTT+TGG + chr4.1:75970547-75970566 None:intergenic 20.0%
! AAATGGAAGATAGTATCAAT+AGG - chr4.1:75971585-75971604 MS.gene87509:intron 25.0%
! AATGGAAGATAGTATCAATA+GGG - chr4.1:75971586-75971605 MS.gene87509:intron 25.0%
! TATTTGGTATATTCAAGAAG+AGG + chr4.1:75970611-75970630 None:intergenic 25.0%
! TTACTGAAGTTATGATGTAA+AGG + chr4.1:75971251-75971270 None:intergenic 25.0%
! TTTGAACATTTCCTTCAAAA+GGG + chr4.1:75971481-75971500 None:intergenic 25.0%
!! AATTTTATCTTCAAGTGGTT+TGG - chr4.1:75971367-75971386 MS.gene87509:CDS 25.0%
!! GAAGTAACAAAATTTTGTGT+TGG + chr4.1:75970943-75970962 None:intergenic 25.0%
!! TTTTGAACATTTCCTTCAAA+AGG + chr4.1:75971482-75971501 None:intergenic 25.0%
!!! AAACTATGGTTTTAGTTACA+TGG - chr4.1:75971304-75971323 MS.gene87509:CDS 25.0%
!!! AACTATGGTTTTAGTTACAT+GGG - chr4.1:75971305-75971324 MS.gene87509:CDS 25.0%
AACCTTAATGATGACATTTG+AGG + chr4.1:75971221-75971240 None:intergenic 30.0%
AATCATGAGATGATAACTAG+TGG - chr4.1:75971500-75971519 MS.gene87509:intron 30.0%
AATTAGCCAGAAAAGGAAAT+TGG + chr4.1:75971433-75971452 None:intergenic 30.0%
ATGGAAGATAGTATCAATAG+GGG - chr4.1:75971587-75971606 MS.gene87509:intron 30.0%
GGAAACAATAACAGTAATCA+AGG - chr4.1:75971629-75971648 MS.gene87509:intron 30.0%
! ATCAAGGAAGTGAACAATTT+TGG - chr4.1:75971645-75971664 MS.gene87509:intron 30.0%
!! AGTAATTTGTACCCTTTTGA+AGG - chr4.1:75971467-75971486 MS.gene87509:intron 30.0%
!! CTTTTGTTTCTTTGTCCAAA+AGG - chr4.1:75970529-75970548 MS.gene87509:CDS 30.0%
!!! ATTTCCTTTTCTGGCTAATT+TGG - chr4.1:75971433-75971452 MS.gene87509:CDS 30.0%
AATTGGACTGATCATGTTTC+TGG - chr4.1:75971701-75971720 MS.gene87509:intron 35.0%
AGGTGTAAGGCCTAATATAT+CGG + chr4.1:75971201-75971220 None:intergenic 35.0%
AGTAACCCTAATGATTTCGT+CGG - chr4.1:75971266-75971285 MS.gene87509:CDS 35.0%
CACCTCAAATGTCATCATTA+AGG - chr4.1:75971216-75971235 MS.gene87509:CDS 35.0%
CCAAAAGTGATTTCAACTTC+TGG - chr4.1:75971536-75971555 MS.gene87509:intron 35.0%
GGAAGAGTGGATATTGTTAT+TGG + chr4.1:75971177-75971196 None:intergenic 35.0%
TCAAGCAAAAGTAGCAACAA+TGG - chr4.1:75971080-75971099 MS.gene87509:CDS 35.0%
! AGAGTGGATATTGTTATTGG+AGG + chr4.1:75971174-75971193 None:intergenic 35.0%
! AGTATCAATAGGGGCTTTTT+GGG - chr4.1:75971596-75971615 MS.gene87509:intron 35.0%
! ATGATGACATTTGAGGTGTA+AGG + chr4.1:75971214-75971233 None:intergenic 35.0%
! CCAGAAGTTGAAATCACTTT+TGG + chr4.1:75971539-75971558 None:intergenic 35.0%
! TAGTATCAATAGGGGCTTTT+TGG - chr4.1:75971595-75971614 MS.gene87509:intron 35.0%
! TCAACTTGCTTCTGTGAAAA+TGG - chr4.1:75971568-75971587 MS.gene87509:intron 35.0%
! TGGAATAGTGCTGTTATTAC+TGG - chr4.1:75971665-75971684 MS.gene87509:intron 35.0%
! TGTTGATGAGTCATTGACAT+TGG + chr4.1:75971407-75971426 None:intergenic 35.0%
!!! GGAGATTTTGGGTTAAACTA+TGG - chr4.1:75971290-75971309 MS.gene87509:CDS 35.0%
!!! TATGGTTTTAGTTACATGGG+AGG - chr4.1:75971308-75971327 MS.gene87509:CDS 35.0%
ATCAAGATAAGCTGCTGGTA+TGG + chr4.1:75970447-75970466 None:intergenic 40.0%
CATGAGATGATAACTAGTGG+TGG - chr4.1:75971503-75971522 MS.gene87509:intron 40.0%
CTGGTAATTCTGCAGCTAAT+TGG - chr4.1:75971684-75971703 MS.gene87509:intron 40.0%
TGGAAACTCAACAAAGTCTC+CGG - chr4.1:75971100-75971119 MS.gene87509:CDS 40.0%
TGTAAGGCCTAATATATCGG+CGG + chr4.1:75971198-75971217 None:intergenic 40.0%
TTTAGTTACATGGGAGGAGT+AGG - chr4.1:75971314-75971333 MS.gene87509:CDS 40.0%
! ACAGCACCAATTTCCTTTTC+TGG - chr4.1:75971424-75971443 MS.gene87509:CDS 40.0%
! ATTTCGTCGGAGGAGATTTT+GGG - chr4.1:75971279-75971298 MS.gene87509:CDS 40.0%
!! TAACTAGTGGTGGATATGTG+AGG - chr4.1:75971513-75971532 MS.gene87509:intron 40.0%
!!! CCTTTTCTGGCTAATTTGGA+GGG - chr4.1:75971437-75971456 MS.gene87509:CDS 40.0%
!!! TCCTTTTCTGGCTAATTTGG+AGG - chr4.1:75971436-75971455 MS.gene87509:CDS 40.0%
AACCCTAATGATTTCGTCGG+AGG - chr4.1:75971269-75971288 MS.gene87509:CDS 45.0%
ACTTCTGCAAGACTTGCAGA+AGG - chr4.1:75970991-75971010 MS.gene87509:CDS 45.0%
ATGGGAGGAGTAGGTGATTT+GGG - chr4.1:75971323-75971342 MS.gene87509:CDS 45.0%
ATTTACCTGTTGCTGCCAGT+TGG + chr4.1:75970474-75970493 None:intergenic 45.0%
CACTCTTCCGCCGATATATT+AGG - chr4.1:75971188-75971207 MS.gene87509:CDS 45.0%
CAGAATCATCATCAGCAACC+AGG - chr4.1:75970732-75970751 MS.gene87509:CDS 45.0%
CAGTCACAATCTCAACCTCA+TGG - chr4.1:75970810-75970829 MS.gene87509:CDS 45.0%
CATCATCAGCAACCAGGAAA+CGG - chr4.1:75970738-75970757 MS.gene87509:CDS 45.0%
CCCTCCAAATTAGCCAGAAA+AGG + chr4.1:75971440-75971459 None:intergenic 45.0%
CTAATATATCGGCGGAAGAG+TGG + chr4.1:75971190-75971209 None:intergenic 45.0%
CTCCTCCGACGAAATCATTA+GGG + chr4.1:75971274-75971293 None:intergenic 45.0%
CTGAAGCAGTTGTTGAGAGA+CGG + chr4.1:75970769-75970788 None:intergenic 45.0%
CTTATCTTGATGCAGCCAAC+TGG - chr4.1:75970456-75970475 MS.gene87509:CDS 45.0%
TCACAATCTCAACCTCATGG+AGG - chr4.1:75970813-75970832 MS.gene87509:CDS 45.0%
TCTCCTCCGACGAAATCATT+AGG + chr4.1:75971275-75971294 None:intergenic 45.0%
TGAGGTTGAGATTGTGACTG+TGG + chr4.1:75970810-75970829 None:intergenic 45.0%
! CTTCAAGTGGTTTGGAGCAA+TGG - chr4.1:75971375-75971394 MS.gene87509:CDS 45.0%
! GATTTCGTCGGAGGAGATTT+TGG - chr4.1:75971278-75971297 MS.gene87509:CDS 45.0%
!! CAAGTCTTGCAGAAGTGTCT+GGG + chr4.1:75970987-75971006 None:intergenic 45.0%
!! GATTTGGGAAGTGCTTTAGG+AGG - chr4.1:75971338-75971357 MS.gene87509:CDS 45.0%
!! GGCTTTTTGGGAATTAGCAC+AGG - chr4.1:75971608-75971627 MS.gene87509:intron 45.0%
!! GGTGATTTGGGAAGTGCTTT+AGG - chr4.1:75971335-75971354 MS.gene87509:CDS 45.0%
!! GTGTTGGTTGATTCACAACG+TGG + chr4.1:75970927-75970946 None:intergenic 45.0%
CAATCTCAACCTCATGGAGG+AGG - chr4.1:75970816-75970835 MS.gene87509:CDS 50.0%
CATGGGAGGAGTAGGTGATT+TGG - chr4.1:75971322-75971341 MS.gene87509:CDS 50.0%
GCTGCATCAAGATAAGCTGC+TGG + chr4.1:75970452-75970471 None:intergenic 50.0%
GGTTGAGATTGTGACTGTGG+TGG + chr4.1:75970807-75970826 None:intergenic 50.0%
TGAGATTGTGACTGTGGTGG+TGG + chr4.1:75970804-75970823 None:intergenic 50.0%
TTGAACTAGCACTCCCCGTT+TGG + chr4.1:75971139-75971158 None:intergenic 50.0%
! CAAGTGGTTTGGAGCAATGG+AGG - chr4.1:75971378-75971397 MS.gene87509:CDS 50.0%
! GCAAGACTTGCAGAAGGTAC+TGG - chr4.1:75970997-75971016 MS.gene87509:CDS 50.0%
!! AGAAGGTACTGGACAAGAGG+CGG - chr4.1:75971008-75971027 MS.gene87509:CDS 50.0%
!! GCAAGTCTTGCAGAAGTGTC+TGG + chr4.1:75970988-75971007 None:intergenic 50.0%
!! TGCAGAAGGTACTGGACAAG+AGG - chr4.1:75971005-75971024 MS.gene87509:CDS 50.0%
AACTAGCACTCCCCGTTTGG+CGG + chr4.1:75971136-75971155 None:intergenic 55.0%
CATCAGCAACCAGGAAACGG+CGG - chr4.1:75970741-75970760 MS.gene87509:CDS 55.0%
CGATCCTGGCCTAATTGAGC+CGG + chr4.1:75970847-75970866 None:intergenic 55.0%
GATCGATGTCCGATAGGGCA+AGG - chr4.1:75970862-75970881 MS.gene87509:CDS 55.0%
GATGGCGAACATGCCTATGC+CGG - chr4.1:75970884-75970903 MS.gene87509:CDS 55.0%
GATGTCCGATAGGGCAAGGA+TGG - chr4.1:75970866-75970885 MS.gene87509:CDS 55.0%
GCCAGGATCGATGTCCGATA+GGG - chr4.1:75970857-75970876 MS.gene87509:CDS 55.0%
GGACATTTCAGTGCTGCCTC+CGG + chr4.1:75970906-75970925 None:intergenic 55.0%
GTTCGCCATCCTTGCCCTAT+CGG + chr4.1:75970874-75970893 None:intergenic 55.0%
!! AAGGTACTGGACAAGAGGCG+GGG - chr4.1:75971010-75971029 MS.gene87509:CDS 55.0%
!! GAAGGTACTGGACAAGAGGC+GGG - chr4.1:75971009-75971028 MS.gene87509:CDS 55.0%
!! GAGGATCCACCGGCTCAATT+AGG - chr4.1:75970835-75970854 MS.gene87509:CDS 55.0%
!! GATTGTGACTGTGGTGGTGG+TGG + chr4.1:75970801-75970820 None:intergenic 55.0%
!! GCTCTAAGGAATGTCCCGGT+CGG - chr4.1:75971032-75971051 MS.gene87509:CDS 55.0%
CCTCATGGAGGAGGATCCAC+CGG - chr4.1:75970825-75970844 MS.gene87509:CDS 60.0%
CTAAGGAATGTCCCGGTCGG+TGG - chr4.1:75971035-75971054 MS.gene87509:CDS 60.0%
GCCCTATCGGACATCGATCC+TGG + chr4.1:75970861-75970880 None:intergenic 60.0%
GGCCAGGATCGATGTCCGAT+AGG - chr4.1:75970856-75970875 MS.gene87509:CDS 60.0%
GTTTGGCGGTCAGAGCTTGC+CGG + chr4.1:75971122-75971141 None:intergenic 60.0%
TCCTGGCCTAATTGAGCCGG+TGG + chr4.1:75970844-75970863 None:intergenic 60.0%
TGCAGCCAACTGGCAGCAAC+AGG - chr4.1:75970466-75970485 MS.gene87509:CDS 60.0%
TTCAGTGCTGCCTCCGGCAT+AGG + chr4.1:75970900-75970919 None:intergenic 60.0%
! CAAGCTCTGACCGCCAAACG+GGG - chr4.1:75971123-75971142 MS.gene87509:CDS 60.0%
! GCAAGCTCTGACCGCCAAAC+GGG - chr4.1:75971122-75971141 MS.gene87509:CDS 60.0%
! GGCAAGCTCTGACCGCCAAA+CGG - chr4.1:75971121-75971140 MS.gene87509:CDS 60.0%
!! CGGGGCTCTAAGGAATGTCC+CGG - chr4.1:75971028-75971047 MS.gene87509:CDS 60.0%
!! TCCACCGGCTCAATTAGGCC+AGG - chr4.1:75970840-75970859 MS.gene87509:CDS 60.0%
!! TGTGACTGTGGTGGTGGTGG+TGG + chr4.1:75970798-75970817 None:intergenic 60.0%
AGGAATGTCCCGGTCGGTGG+AGG - chr4.1:75971038-75971057 MS.gene87509:CDS 65.0%
CCGGTGGATCCTCCTCCATG+AGG + chr4.1:75970828-75970847 None:intergenic 65.0%
CTCCGAAAGCCTCCACCGAC+CGG + chr4.1:75971050-75971069 None:intergenic 65.0%
GGACAAGAGGCGGGGCTCTA+AGG - chr4.1:75971018-75971037 MS.gene87509:CDS 65.0%
GGCGAACATGCCTATGCCGG+AGG - chr4.1:75970887-75970906 MS.gene87509:CDS 65.0%
TCCCGGTCGGTGGAGGCTTT+CGG - chr4.1:75971045-75971064 MS.gene87509:CDS 65.0%
TCCGAAAGCCTCCACCGACC+GGG + chr4.1:75971049-75971068 None:intergenic 65.0%
!! GACTGTGGTGGTGGTGGTGG+TGG + chr4.1:75970795-75970814 None:intergenic 65.0%
GAGACGGAGCCGCCGTTTCC+TGG + chr4.1:75970753-75970772 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 75970431 75971763 75970431 ID=MS.gene87509
chr4.1 mRNA 75970431 75971763 75970431 ID=MS.gene87509.t1;Parent=MS.gene87509
chr4.1 exon 75971707 75971763 75971707 ID=MS.gene87509.t1.exon1;Parent=MS.gene87509.t1
chr4.1 CDS 75971707 75971763 75971707 ID=cds.MS.gene87509.t1;Parent=MS.gene87509.t1
chr4.1 exon 75970431 75971465 75970431 ID=MS.gene87509.t1.exon2;Parent=MS.gene87509.t1
chr4.1 CDS 75970431 75971465 75970431 ID=cds.MS.gene87509.t1;Parent=MS.gene87509.t1
Gene Sequence

>MS.gene87509

ATGGTTTTTACTTCCATACCAGCAGCTTATCTTGATGCAGCCAACTGGCAGCAACAGGTAAATATAATTAGTAAGGTTTCAATTTAATTTGGTTCTTGCTTTTGTTTCTTTGTCCAAAAGGCAAAATAATTCAAAAGATAAAAAGGAAAAACAATATTCTTGTTACTTTTATTTATACCTCTTCTTGAATATACCAAATATTTGATTTCTTTCTTATTACCTTTCTTCTATTATTCTCAATTCATATACTCTTTTTCTAATTTTTCTTCCTTTTTGTCTATTTATGTTGTTATTTCAGCAACAGAATCATCATCAGCAACCAGGAAACGGCGGCTCCGTCTCTCAACAACTGCTTCAGACACCACCACCACCACCACCACAGTCACAATCTCAACCTCATGGAGGAGGATCCACCGGCTCAATTAGGCCAGGATCGATGTCCGATAGGGCAAGGATGGCGAACATGCCTATGCCGGAGGCAGCACTGAAATGTCCACGTTGTGAATCAACCAACACAAAATTTTGTTACTTCAACAACTACAGTCTCTCTCAGCCCAGACACTTCTGCAAGACTTGCAGAAGGTACTGGACAAGAGGCGGGGCTCTAAGGAATGTCCCGGTCGGTGGAGGCTTTCGGAGAAACAAGAGATCAAGCAAAAGTAGCAACAATGGAAACTCAACAAAGTCTCCGGCAAGCTCTGACCGCCAAACGGGGAGTGCTAGTTCAACAAACTCAATCACCTCCAATAACAATATCCACTCTTCCGCCGATATATTAGGCCTTACACCTCAAATGTCATCATTAAGGTTCATGACACCTTTACATCATAACTTCAGTAACCCTAATGATTTCGTCGGAGGAGATTTTGGGTTAAACTATGGTTTTAGTTACATGGGAGGAGTAGGTGATTTGGGAAGTGCTTTAGGAGGTAATTCAATTTTATCTTCAAGTGGTTTGGAGCAATGGAGGATGCCAATGTCAATGACTCATCAACAGCACCAATTTCCTTTTCTGGCTAATTTGGAGGGTTCAAATAGTAATTTGTACCCTTTTGAAGGAAATGTTCAAAATCATGAGATGATAACTAGTGGTGGATATGTGAGGCCAAAAGTGATTTCAACTTCTGGAATTATGACTCAACTTGCTTCTGTGAAAATGGAAGATAGTATCAATAGGGGCTTTTTGGGAATTAGCACAGGAAACAATAACAGTAATCAAGGAAGTGAACAATTTTGGAATAGTGCTGTTATTACTGGTAATTCTGCAGCTAATTGGACTGATCATGTTTCTGGTTTTAGCTCTTCTTCTAATACTACTAGTAACCAGATGTAG
Protein sequence

>MS.gene87509.t1

MVFTSIPAAYLDAANWQQQQQNHHQQPGNGGSVSQQLLQTPPPPPPQSQSQPHGGGSTGSIRPGSMSDRARMANMPMPEAALKCPRCESTNTKFCYFNNYSLSQPRHFCKTCRRYWTRGGALRNVPVGGGFRRNKRSSKSSNNGNSTKSPASSDRQTGSASSTNSITSNNNIHSSADILGLTPQMSSLRFMTPLHHNFSNPNDFVGGDFGLNYGFSYMGGVGDLGSALGGNSILSSSGLEQWRMPMSMTHQQHQFPFLANLEGSNSNLYPFEGNVQNHEMITSGGYVRPKVISTSGIMTQLASVKMEDSINRGFLGISTGNNNSNQGSEQFWNSAVITGNSAANWTDHVSGFSSSSNTTSNQM