AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene87542

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene87542.t1 MTR_1g098000 89.855 207 12 1 55 252 36 242 6.55e-134 387
MS.gene87542.t1 MTR_1g098370 89.372 207 13 1 55 252 39 245 8.26e-133 384
MS.gene87542.t1 MTR_3g088625 41.872 203 103 4 56 246 1161 1360 4.44e-46 165
MS.gene87542.t1 MTR_3g088625 41.872 203 103 4 56 246 1267 1466 5.47e-46 164
MS.gene87542.t1 MTR_6g059310 39.512 205 112 4 52 247 52 253 2.41e-37 134
MS.gene87542.t1 MTR_6g059310 39.512 205 112 4 52 247 52 253 2.46e-37 134
MS.gene87542.t1 MTR_0071s0100 36.735 196 105 7 69 249 162 353 7.12e-30 115
MS.gene87542.t1 MTR_7g017100 98.113 53 1 0 1 53 2498 2550 1.51e-26 108
MS.gene87542.t1 MTR_2g036060 35.294 136 86 2 104 239 1057 1190 4.37e-19 87.0
MS.gene87542.t1 MTR_2g036060 35.294 136 86 2 104 239 1032 1165 4.53e-19 86.7
MS.gene87542.t1 MTR_8g078490 33.333 180 91 6 91 244 1076 1252 1.90e-18 85.1
MS.gene87542.t1 MTR_5g016870 35.928 167 82 4 74 224 674 831 4.08e-18 84.0
MS.gene87542.t1 MTR_5g018850 32.759 174 92 5 91 240 1330 1502 3.81e-17 81.3
MS.gene87542.t1 MTR_7g055660 42.718 103 57 1 121 223 598 698 1.32e-16 79.3
MS.gene87542.t1 MTR_3g091310 38.017 121 71 3 128 245 944 1063 1.67e-16 79.3
MS.gene87542.t1 MTR_3g091310 38.017 121 71 3 128 245 850 969 1.68e-16 79.3
MS.gene87542.t1 MTR_8g027725 38.017 121 71 3 128 245 815 934 2.28e-16 79.0
MS.gene87542.t1 MTR_8g027725 38.017 121 71 3 128 245 830 949 2.42e-16 78.6
MS.gene87542.t1 MTR_4g132610 33.523 176 88 6 91 240 870 1042 2.79e-16 78.6
MS.gene87542.t1 MTR_1g008230 38.017 121 71 3 128 245 637 756 2.97e-16 78.6
MS.gene87542.t1 MTR_1g008230 38.017 121 71 3 128 245 915 1034 3.49e-16 78.2
MS.gene87542.t1 MTR_1g012890 36.585 123 73 2 128 245 246 368 6.21e-16 76.6
MS.gene87542.t1 MTR_7g021365 39.130 115 68 2 127 240 927 1040 1.85e-15 76.3
MS.gene87542.t1 MTR_1g012910 35.772 123 74 2 128 245 549 671 1.96e-15 75.9
MS.gene87542.t1 MTR_1g086980 42.574 101 56 1 121 221 678 776 2.55e-15 75.5
MS.gene87542.t1 MTR_1g086980 42.574 101 56 1 121 221 498 596 2.59e-15 75.5
MS.gene87542.t1 MTR_1g086980 42.574 101 56 1 121 221 688 786 2.61e-15 75.5
MS.gene87542.t1 MTR_1g012910 35.772 123 74 2 128 245 386 508 2.62e-15 75.5
MS.gene87542.t1 MTR_1g086980 42.574 101 56 1 121 221 647 745 2.83e-15 75.5
MS.gene87542.t1 MTR_1g086980 42.574 101 56 1 121 221 698 796 2.85e-15 75.5
MS.gene87542.t1 MTR_1g086980 42.574 101 56 1 121 221 626 724 2.86e-15 75.5
MS.gene87542.t1 MTR_7g117355 38.710 124 66 4 128 245 845 964 3.24e-15 75.5
MS.gene87542.t1 MTR_8g078505 31.395 172 94 5 91 240 364 533 5.55e-15 74.3
MS.gene87542.t1 MTR_5g013420 31.313 198 89 8 91 244 894 1088 4.62e-14 72.0
MS.gene87542.t1 MTR_1g048950 34.395 157 75 7 105 241 381 529 1.40e-13 70.5
MS.gene87542.t1 MTR_5g013420 30.769 195 88 8 91 241 894 1085 5.79e-13 68.9
MS.gene87542.t1 MTR_4g010830 29.146 199 86 8 91 241 785 976 1.07e-12 67.8
MS.gene87542.t1 MTR_7g088370 29.348 184 99 6 91 244 522 704 1.28e-12 67.8
MS.gene87542.t1 MTR_8g042750 28.986 207 88 8 91 245 489 688 1.81e-12 67.0
MS.gene87542.t1 MTR_7g109560 38.462 104 62 1 121 224 628 729 4.00e-12 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene87542.t1 AT1G76710 74.038 208 45 1 54 252 29 236 2.22e-111 329
MS.gene87542.t1 AT1G76710 74.038 208 45 1 54 252 29 236 2.22e-111 329
MS.gene87542.t1 AT1G76710 74.038 208 45 1 54 252 29 236 5.69e-111 328
MS.gene87542.t1 AT1G76710 74.038 208 45 1 54 252 29 236 8.12e-111 329
MS.gene87542.t1 AT1G77300 42.647 204 102 4 55 246 968 1168 2.59e-46 166
MS.gene87542.t1 AT1G77300 42.647 204 102 4 55 246 968 1168 2.95e-46 165
MS.gene87542.t1 AT4G30860 37.778 180 99 4 79 249 294 469 7.42e-29 114
MS.gene87542.t1 AT2G44150 40.132 152 81 2 105 247 107 257 1.84e-26 105
MS.gene87542.t1 AT3G59960 38.947 190 84 7 84 247 69 252 2.63e-26 104
MS.gene87542.t1 AT3G59960 38.947 190 84 7 84 247 69 252 2.63e-26 104
MS.gene87542.t1 AT3G59960 38.947 190 84 7 84 247 69 252 2.63e-26 104
MS.gene87542.t1 AT3G59960 38.172 186 91 4 84 247 69 252 6.27e-26 103
MS.gene87542.t1 AT5G42400 41.228 114 66 1 127 240 1271 1383 3.88e-22 95.9
MS.gene87542.t1 AT5G42400 41.228 114 66 1 127 240 1271 1383 3.88e-22 95.9
MS.gene87542.t1 AT5G42400 41.228 114 66 1 127 240 1271 1383 3.88e-22 95.9
MS.gene87542.t1 AT5G42400 41.228 114 66 1 127 240 1271 1383 3.88e-22 95.9
MS.gene87542.t1 AT5G42400 41.228 114 66 1 127 240 1271 1383 3.88e-22 95.9
MS.gene87542.t1 AT5G42400 41.228 114 66 1 127 240 1271 1383 3.88e-22 95.9
MS.gene87542.t1 AT5G42400 41.228 114 66 1 127 240 1271 1383 3.88e-22 95.9
MS.gene87542.t1 AT5G42400 41.228 114 66 1 127 240 1271 1383 3.88e-22 95.9
MS.gene87542.t1 AT1G05830 39.130 115 68 2 127 240 941 1054 3.89e-17 81.3
MS.gene87542.t1 AT1G05830 39.130 115 68 2 127 240 941 1054 3.89e-17 81.3
MS.gene87542.t1 AT1G05830 39.130 115 68 2 127 240 941 1054 3.89e-17 81.3
MS.gene87542.t1 AT1G05830 39.130 115 68 2 127 240 941 1054 3.89e-17 81.3
MS.gene87542.t1 AT4G27910 38.843 121 70 3 128 245 908 1027 1.07e-16 79.7
MS.gene87542.t1 AT2G23380 44.792 96 51 1 129 224 549 642 2.68e-16 78.6
MS.gene87542.t1 AT2G23380 43.269 104 57 1 121 224 768 869 2.70e-16 78.6
MS.gene87542.t1 AT1G02580 31.522 184 100 5 78 247 518 689 5.67e-16 77.4
MS.gene87542.t1 AT2G31650 39.130 115 68 2 127 240 920 1033 6.16e-16 77.8
MS.gene87542.t1 AT3G02260 66.667 54 18 0 1 54 2503 2556 6.85e-16 77.8
MS.gene87542.t1 AT3G61740 39.024 123 68 4 128 245 862 982 1.80e-15 76.3
MS.gene87542.t1 AT4G02020 35.802 162 85 6 74 223 669 823 1.90e-15 76.3
MS.gene87542.t1 AT3G61740 39.024 123 68 4 128 245 898 1018 1.98e-15 76.3
MS.gene87542.t1 AT3G02260 66.038 53 18 0 1 53 2487 2539 4.27e-15 75.5
MS.gene87542.t1 AT3G02260 66.038 53 18 0 1 53 2496 2548 4.27e-15 75.5
MS.gene87542.t1 AT3G02260 66.038 53 18 0 1 53 2486 2538 4.35e-15 75.5
MS.gene87542.t1 AT5G53430 37.190 121 72 3 128 245 924 1043 2.84e-14 72.8
MS.gene87542.t1 AT3G03750 32.222 180 93 7 89 240 167 345 1.98e-12 66.6

Find 48 sgRNAs with CRISPR-Local

Find 154 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GACCTTGAGTTTCATAAGTA+TGG 0.226901 4.1:+75495036 None:intergenic
CAAGATTTATAAACCATTCC+TGG 0.303845 4.1:-75494864 MS.gene87542:intron
GCACTGGAAGATATTCCTAT+TGG 0.334570 4.1:-75494643 MS.gene87542:CDS
GCCCTCCATGTAAAAGATTC+AGG 0.335082 4.1:-75497163 MS.gene87542:CDS
AGACATAACAATGTTGTTCA+TGG 0.337645 4.1:+75497222 None:intergenic
CAAACTGTGAGACAAGAAAA+TGG 0.346413 4.1:-75494699 MS.gene87542:CDS
CCTGACAGTGCCTGTGGAGA+TGG 0.413253 4.1:-75496017 MS.gene87542:CDS
TTCTTTAGTAATACAACAGC+AGG 0.423433 4.1:+75497124 None:intergenic
CTCAAATGTTCTGAATTCCT+TGG 0.432418 4.1:-75494550 MS.gene87542:CDS
ATGCCACAAGGGCAAAATCC+AGG 0.434511 4.1:+75495960 None:intergenic
CAGTTTGTCATTGAATACTT+TGG 0.459466 4.1:-75495095 MS.gene87542:CDS
TATTCCTATTGGAACTGAAC+TGG 0.465129 4.1:-75494632 MS.gene87542:CDS
TTGAAGTAGTTTACCAGGAA+TGG 0.466171 4.1:+75494851 None:intergenic
TATGCCAGTTCAGTTCCAAT+AGG 0.481653 4.1:+75494628 None:intergenic
TTATTGTTATGCTGAATGCT+TGG 0.483188 4.1:-75497185 MS.gene87542:CDS
TAAACTGAAGGCCGAGGGTG+GGG 0.484563 4.1:-75495252 MS.gene87542:intron
ACCTTGAGTTTCATAAGTAT+GGG 0.487157 4.1:+75495037 None:intergenic
CTGCAGGCATAAGAAGCAAA+AGG 0.490559 4.1:-75496084 MS.gene87542:intron
TCTATCGATGCAACTAGAAA+AGG 0.490560 4.1:-75494895 MS.gene87542:CDS
CTGACAGTGCCTGTGGAGAT+GGG 0.490971 4.1:-75496016 MS.gene87542:CDS
CCATCTCCACAGGCACTGTC+AGG 0.494885 4.1:+75496017 None:intergenic
TCCAGGGTTGCATTCAGTGC+TGG 0.501130 4.1:+75495977 None:intergenic
ATAAGTATGGGATCTGCACT+TGG 0.504170 4.1:+75495049 None:intergenic
AACAAAGTTATTTAAACTGA+AGG 0.512234 4.1:-75495264 MS.gene87542:CDS
AAGAGTTGGAATATTTGCAC+TGG 0.522066 4.1:-75494659 MS.gene87542:CDS
AAGCTATTCAAACTGCTAGC+AGG 0.522237 4.1:-75497081 MS.gene87542:intron
AATGGTTTATAAATCTTGCA+AGG 0.536559 4.1:+75494869 None:intergenic
CAGCCAAAAGACCCCACCCT+CGG 0.537785 4.1:+75495241 None:intergenic
ACCAGCACTGAATGCAACCC+TGG 0.543451 4.1:-75495978 MS.gene87542:CDS
ACAAGAAAATGGAATGTCAT+GGG 0.544715 4.1:-75494688 MS.gene87542:CDS
GTTATTTAAACTGAAGGCCG+AGG 0.556676 4.1:-75495258 MS.gene87542:CDS
TGAATGGTTTGGAGGTGCAA+AGG 0.560782 4.1:-75494593 MS.gene87542:CDS
GACAAGAAAATGGAATGTCA+TGG 0.568296 4.1:-75494689 MS.gene87542:CDS
TTAAACTGAAGGCCGAGGGT+GGG 0.569938 4.1:-75495253 MS.gene87542:CDS
TTTAAACTGAAGGCCGAGGG+TGG 0.570392 4.1:-75495254 MS.gene87542:CDS
ATATTGCTATATGTGAATGC+AGG 0.572676 4.1:-75496055 MS.gene87542:CDS
TGCCACAAGGGCAAAATCCA+GGG 0.579516 4.1:+75495961 None:intergenic
TTCCTTGGAGCAAAATCTCG+TGG 0.586918 4.1:-75494535 MS.gene87542:CDS
GCATAAGAAGCAAAAGGAGG+AGG 0.601781 4.1:-75496078 MS.gene87542:CDS
TTATTTAAACTGAAGGCCGA+GGG 0.622157 4.1:-75495257 MS.gene87542:CDS
TCCCATACTTATGAAACTCA+AGG 0.623281 4.1:-75495038 MS.gene87542:intron
GCAAAGGTTCGCTGCCTCTG+TGG 0.623435 4.1:-75494577 MS.gene87542:CDS
ATTTAGACACCCATCTCCAC+AGG 0.625505 4.1:+75496007 None:intergenic
CAAGAAAATGGAATGTCATG+GGG 0.642373 4.1:-75494687 MS.gene87542:CDS
GATGATCCTGACAGTGCCTG+TGG 0.657570 4.1:-75496023 MS.gene87542:CDS
AACATTTGAGCGCACCACAG+AGG 0.676482 4.1:+75494563 None:intergenic
CAGGCATAAGAAGCAAAAGG+AGG 0.695576 4.1:-75496081 MS.gene87542:CDS
AAGAAAATGGAATGTCATGG+GGG 0.698806 4.1:-75494686 MS.gene87542:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATATATTTTTTGTATAGTA+TGG - chr4.1:75495318-75495337 MS.gene87542:intron 10.0%
!!! TAAATTTTGTGTAATATTTA+AGG - chr4.1:75495289-75495308 MS.gene87542:CDS 10.0%
!!! TTGGTTTTTTAATTATTTTT+AGG + chr4.1:75494817-75494836 None:intergenic 10.0%
!! AAAAAGATAAGTAAAAAGAA+CGG + chr4.1:75494986-75495005 None:intergenic 15.0%
!! AAAGAAAAATAACCATATTA+AGG + chr4.1:75496558-75496577 None:intergenic 15.0%
!! AAATTAAGGTAATCATATAA+TGG - chr4.1:75496521-75496540 MS.gene87542:intron 15.0%
!! AATTAAGGTAATCATATAAT+GGG - chr4.1:75496522-75496541 MS.gene87542:intron 15.0%
!! GGTAAATTTCATTTAATATT+AGG + chr4.1:75496916-75496935 None:intergenic 15.0%
!! AACAAAGTTATTTAAACTGA+AGG - chr4.1:75496469-75496488 MS.gene87542:intron 20.0%
!! ATTAAGGTAATCATATAATG+GGG - chr4.1:75496523-75496542 MS.gene87542:intron 20.0%
!! TTAGTAAGAAAATCATTAGT+AGG + chr4.1:75494842-75494861 None:intergenic 20.0%
!! TTTGTTTGTTTATTTCATAC+AGG - chr4.1:75496610-75496629 MS.gene87542:intron 20.0%
!!! AAAAAGTATGCAATGAAAAT+AGG + chr4.1:75496085-75496104 None:intergenic 20.0%
!!! ATTTTTAGGAAAAGAGTATT+GGG + chr4.1:75494803-75494822 None:intergenic 20.0%
!!! CATATGACTATAATTTTGAA+TGG - chr4.1:75497124-75497143 MS.gene87542:CDS 20.0%
!!! TATTTTTAGGAAAAGAGTAT+TGG + chr4.1:75494804-75494823 None:intergenic 20.0%
!!! TTATTCTAACTTTGATAATC+AGG - chr4.1:75494699-75494718 MS.gene87542:CDS 20.0%
! AAATAGGAAGATAACAAAAC+AGG + chr4.1:75496962-75496981 None:intergenic 25.0%
! AATGGTTTATAAATCTTGCA+AGG + chr4.1:75496867-75496886 None:intergenic 25.0%
! ACATTAAGATTTCTCAAGAA+TGG + chr4.1:75495596-75495615 None:intergenic 25.0%
! AGAAAATCATTAGTAGGTAT+TGG + chr4.1:75494836-75494855 None:intergenic 25.0%
! GAAAACGAGAATATTTCTTT+AGG + chr4.1:75496370-75496389 None:intergenic 25.0%
! TTATAACTTAGAAGGCTATT+GGG - chr4.1:75494731-75494750 MS.gene87542:intron 25.0%
!! ATCATATAATGGGGAAATTT+AGG - chr4.1:75496532-75496551 MS.gene87542:intron 25.0%
!! ATCTGAAGTAAAGTTTAAGT+AGG + chr4.1:75496119-75496138 None:intergenic 25.0%
!! GACTATAATTTTGAATGGTT+TGG - chr4.1:75497129-75497148 MS.gene87542:CDS 25.0%
!!! ACTTTGATAATCAGGTTTTT+AGG - chr4.1:75494707-75494726 MS.gene87542:CDS 25.0%
!!! CGTAAGACTTTTTTTTTTGA+AGG - chr4.1:75495217-75495236 MS.gene87542:intron 25.0%
!!! CTTTGATAATCAGGTTTTTA+GGG - chr4.1:75494708-75494727 MS.gene87542:intron 25.0%
!!! TATAATTTTGAATGGTTTGG+AGG - chr4.1:75497132-75497151 MS.gene87542:CDS 25.0%
!!! TGTTGCATATTTTTTTTGAG+AGG - chr4.1:75495961-75495980 MS.gene87542:CDS 25.0%
!!! TTTTAGGGTTATAACTTAGA+AGG - chr4.1:75494723-75494742 MS.gene87542:intron 25.0%
ACAAGAAAATGGAATGTCAT+GGG - chr4.1:75497045-75497064 MS.gene87542:intron 30.0%
ACCTTGAGTTTCATAAGTAT+GGG + chr4.1:75496699-75496718 None:intergenic 30.0%
ACTTGTAGAACAAAATAACC+AGG + chr4.1:75496018-75496037 None:intergenic 30.0%
ATATTGCTATATGTGAATGC+AGG - chr4.1:75495678-75495697 MS.gene87542:intron 30.0%
CAAATAAATAGATGAGCGTA+TGG + chr4.1:75496594-75496613 None:intergenic 30.0%
CAAGATTTATAAACCATTCC+TGG - chr4.1:75496869-75496888 MS.gene87542:intron 30.0%
CAGTTTGTCATTGAATACTT+TGG - chr4.1:75496638-75496657 MS.gene87542:intron 30.0%
CTTGTAGAACAAAATAACCA+GGG + chr4.1:75496017-75496036 None:intergenic 30.0%
GAAATTATACGTAAGACTTG+TGG - chr4.1:75495239-75495258 MS.gene87542:intron 30.0%
GCTATAGATATCATTGCAAT+CGG - chr4.1:75496322-75496341 MS.gene87542:intron 30.0%
GTTATAACTTAGAAGGCTAT+TGG - chr4.1:75494730-75494749 MS.gene87542:intron 30.0%
TAAAACCATAGTTGTCAAAG+CGG + chr4.1:75495446-75495465 None:intergenic 30.0%
TTATTGTTATGCTGAATGCT+TGG - chr4.1:75494548-75494567 MS.gene87542:CDS 30.0%
TTCTAGTCAGTTAACTTAAC+TGG - chr4.1:75496204-75496223 MS.gene87542:intron 30.0%
TTCTTTAGTAATACAACAGC+AGG + chr4.1:75494612-75494631 None:intergenic 30.0%
TTTGTTTATTTCATACAGGC+AGG - chr4.1:75496614-75496633 MS.gene87542:intron 30.0%
! AACTCACCTGATTTTTACAA+AGG + chr4.1:75495799-75495818 None:intergenic 30.0%
! AACTTTTCTTCTAAGTAGTG+CGG - chr4.1:75496300-75496319 MS.gene87542:intron 30.0%
! ATTCAGTTGATATCAAACCT+TGG + chr4.1:75496993-75497012 None:intergenic 30.0%
! TTCAGTTGATATCAAACCTT+GGG + chr4.1:75496992-75497011 None:intergenic 30.0%
! TTTGTTTGAAGTAGTTTACC+AGG + chr4.1:75496890-75496909 None:intergenic 30.0%
!! AAACTTTTGTTTGCTTTTGC+AGG - chr4.1:75496775-75496794 MS.gene87542:intron 30.0%
AAGAAAATGGAATGTCATGG+GGG - chr4.1:75497047-75497066 MS.gene87542:intron 35.0%
AAGAGTTGGAATATTTGCAC+TGG - chr4.1:75497074-75497093 MS.gene87542:intron 35.0%
CAAACTGTGAGACAAGAAAA+TGG - chr4.1:75497034-75497053 MS.gene87542:intron 35.0%
CAAGAAAATGGAATGTCATG+GGG - chr4.1:75497046-75497065 MS.gene87542:intron 35.0%
GACAAGAAAATGGAATGTCA+TGG - chr4.1:75497044-75497063 MS.gene87542:intron 35.0%
GACCTTGAGTTTCATAAGTA+TGG + chr4.1:75496700-75496719 None:intergenic 35.0%
GGGAAATTTAGGCCTTAATA+TGG - chr4.1:75496543-75496562 MS.gene87542:intron 35.0%
GGTCTACAATACTTGCATAT+TGG + chr4.1:75496941-75496960 None:intergenic 35.0%
GTTAACTTAACTGGCATAAC+TGG - chr4.1:75496213-75496232 MS.gene87542:intron 35.0%
TACAATACTTGCATATTGGC+AGG + chr4.1:75496937-75496956 None:intergenic 35.0%
TAGCAGTCTGTAGTTACAAT+CGG - chr4.1:75496266-75496285 MS.gene87542:intron 35.0%
TATTCCTATTGGAACTGAAC+TGG - chr4.1:75497101-75497120 MS.gene87542:CDS 35.0%
TATTTGTATTCTGCTTTCCC+TGG - chr4.1:75495997-75496016 MS.gene87542:CDS 35.0%
TCCCATACTTATGAAACTCA+AGG - chr4.1:75496695-75496714 MS.gene87542:intron 35.0%
TCTATCGATGCAACTAGAAA+AGG - chr4.1:75496838-75496857 MS.gene87542:intron 35.0%
TGGCATCCTTTGTAAAAATC+AGG - chr4.1:75495790-75495809 MS.gene87542:intron 35.0%
TTATTTAAACTGAAGGCCGA+GGG - chr4.1:75496476-75496495 MS.gene87542:intron 35.0%
TTGAAGTAGTTTACCAGGAA+TGG + chr4.1:75496885-75496904 None:intergenic 35.0%
TTTGGCTGATGAGGAAATTA+AGG - chr4.1:75496507-75496526 MS.gene87542:intron 35.0%
! CATTTTCTTGTCTCACAGTT+TGG + chr4.1:75497036-75497055 None:intergenic 35.0%
! CTCAAATGTTCTGAATTCCT+TGG - chr4.1:75497183-75497202 MS.gene87542:CDS 35.0%
! TCCTGAATCTTTTACATGGA+GGG + chr4.1:75494574-75494593 None:intergenic 35.0%
! TTTTACAAAGGATGCCACAA+GGG + chr4.1:75495787-75495806 None:intergenic 35.0%
! TTTTTACAAAGGATGCCACA+AGG + chr4.1:75495788-75495807 None:intergenic 35.0%
AAACATGTAAAGATGGGGCA+TGG - chr4.1:75494756-75494775 MS.gene87542:intron 40.0%
AAATCAGGTGAGTTCGTGTT+TGG - chr4.1:75495805-75495824 MS.gene87542:intron 40.0%
AACATGTAAAGATGGGGCAT+GGG - chr4.1:75494757-75494776 MS.gene87542:intron 40.0%
AAGCTATTCAAACTGCTAGC+AGG - chr4.1:75494652-75494671 MS.gene87542:CDS 40.0%
AATCAGGTGAGTTCGTGTTT+GGG - chr4.1:75495806-75495825 MS.gene87542:intron 40.0%
ACTTCTCTTTATCTCTCTGC+AGG - chr4.1:75495633-75495652 MS.gene87542:intron 40.0%
ATAAGTATGGGATCTGCACT+TGG + chr4.1:75496687-75496706 None:intergenic 40.0%
ATGCCCCATCTTTACATGTT+TGG + chr4.1:75494757-75494776 None:intergenic 40.0%
ATTTCTGCAAGCATGAGACA+TGG + chr4.1:75496427-75496446 None:intergenic 40.0%
CCTGCAGGTAGTTAAAAAGT+TGG - chr4.1:75495872-75495891 MS.gene87542:intron 40.0%
GCACTGGAAGATATTCCTAT+TGG - chr4.1:75497090-75497109 MS.gene87542:CDS 40.0%
GCTTTAACATGTGTCTCTGA+AGG - chr4.1:75495924-75495943 MS.gene87542:intron 40.0%
GTTATTTAAACTGAAGGCCG+AGG - chr4.1:75496475-75496494 MS.gene87542:intron 40.0%
TATGCCAGTTCAGTTCCAAT+AGG + chr4.1:75497108-75497127 None:intergenic 40.0%
TGGGCCAAACATGTAAAGAT+GGG - chr4.1:75494750-75494769 MS.gene87542:intron 40.0%
TTGGGCCAAACATGTAAAGA+TGG - chr4.1:75494749-75494768 MS.gene87542:intron 40.0%
! CTCCTGAATCTTTTACATGG+AGG + chr4.1:75494575-75494594 None:intergenic 40.0%
! GCACTCCTGAATCTTTTACA+TGG + chr4.1:75494578-75494597 None:intergenic 40.0%
!! CCAACTTTTTAACTACCTGC+AGG + chr4.1:75495875-75495894 None:intergenic 40.0%
!! CTTTTTAACTACCTGCAGGA+TGG + chr4.1:75495871-75495890 None:intergenic 40.0%
!! GTTTGCTTTTGCAGGTCTTA+AGG - chr4.1:75496783-75496802 MS.gene87542:intron 40.0%
!!! TTTTTTGAGATGCGAGTTGC+AGG - chr4.1:75495154-75495173 MS.gene87542:intron 40.0%
ATTTAGACACCCATCTCCAC+AGG + chr4.1:75495729-75495748 None:intergenic 45.0%
CAGGCATAAGAAGCAAAAGG+AGG - chr4.1:75495652-75495671 MS.gene87542:intron 45.0%
CGTTTCCGCTTTGACAACTA+TGG - chr4.1:75495438-75495457 MS.gene87542:intron 45.0%
CTGCAGGCATAAGAAGCAAA+AGG - chr4.1:75495649-75495668 MS.gene87542:intron 45.0%
GATATCAAACCTTGGGCAGT+GGG + chr4.1:75496985-75497004 None:intergenic 45.0%
GCATAAGAAGCAAAAGGAGG+AGG - chr4.1:75495655-75495674 MS.gene87542:intron 45.0%
GCCCTCCATGTAAAAGATTC+AGG - chr4.1:75494570-75494589 MS.gene87542:CDS 45.0%
GGGCCAAACATGTAAAGATG+GGG - chr4.1:75494751-75494770 MS.gene87542:intron 45.0%
GTCATGGGGGAAATAAGAGT+TGG - chr4.1:75497060-75497079 MS.gene87542:intron 45.0%
TAGTTGTCAAAGCGGAAACG+CGG + chr4.1:75495438-75495457 None:intergenic 45.0%
TGATATCAAACCTTGGGCAG+TGG + chr4.1:75496986-75497005 None:intergenic 45.0%
TGCATCATACACAACAGGCA+AGG + chr4.1:75495508-75495527 None:intergenic 45.0%
TGCCTGTTGTGTATGATGCA+GGG - chr4.1:75495508-75495527 MS.gene87542:intron 45.0%
TTCCTTGGAGCAAAATCTCG+TGG - chr4.1:75497198-75497217 MS.gene87542:CDS 45.0%
TTGCAGTATAACCATCCTGC+AGG - chr4.1:75495857-75495876 MS.gene87542:intron 45.0%
TTGCCTGTTGTGTATGATGC+AGG - chr4.1:75495507-75495526 MS.gene87542:intron 45.0%
! AACCACGAGATTTTGCTCCA+AGG + chr4.1:75497203-75497222 None:intergenic 45.0%
! TGAATGGTTTGGAGGTGCAA+AGG - chr4.1:75497140-75497159 MS.gene87542:CDS 45.0%
AACATTTGAGCGCACCACAG+AGG + chr4.1:75497173-75497192 None:intergenic 50.0%
AACCTTGGGCAGTGGGAAAT+AGG + chr4.1:75496978-75496997 None:intergenic 50.0%
ATGCCACAAGGGCAAAATCC+AGG + chr4.1:75495776-75495795 None:intergenic 50.0%
GACAAGAGCTGTGTCTTCAG+AGG - chr4.1:75495542-75495561 MS.gene87542:intron 50.0%
GAGCTGTGTCTTCAGAGGAA+TGG - chr4.1:75495547-75495566 MS.gene87542:intron 50.0%
GCCTGTTGTGTATGATGCAG+GGG - chr4.1:75495509-75495528 MS.gene87542:intron 50.0%
TGCCACAAGGGCAAAATCCA+GGG + chr4.1:75495775-75495794 None:intergenic 50.0%
TTAAACTGAAGGCCGAGGGT+GGG - chr4.1:75496480-75496499 MS.gene87542:intron 50.0%
TTCCTATTTCCCACTGCCCA+AGG - chr4.1:75496973-75496992 MS.gene87542:intron 50.0%
TTGTGTATGATGCAGGGGCT+AGG - chr4.1:75495514-75495533 MS.gene87542:intron 50.0%
TTTAAACTGAAGGCCGAGGG+TGG - chr4.1:75496479-75496498 MS.gene87542:intron 50.0%
! AACCCTGGATTTTGCCCTTG+TGG - chr4.1:75495770-75495789 MS.gene87542:intron 50.0%
CTGACAGTGCCTGTGGAGAT+GGG - chr4.1:75495717-75495736 MS.gene87542:intron 55.0%
GATGATCCTGACAGTGCCTG+TGG - chr4.1:75495710-75495729 MS.gene87542:intron 55.0%
GCCCCTGCATCATACACAAC+AGG + chr4.1:75495513-75495532 None:intergenic 55.0%
GCGGGAGCTATTACATGACG+CGG + chr4.1:75495370-75495389 None:intergenic 55.0%
GGCCACCCCAAAAATTGACG+CGG + chr4.1:75495412-75495431 None:intergenic 55.0%
TAAACTGAAGGCCGAGGGTG+GGG - chr4.1:75496481-75496500 MS.gene87542:intron 55.0%
TCCAGGGTTGCATTCAGTGC+TGG + chr4.1:75495759-75495778 None:intergenic 55.0%
! ACCAGCACTGAATGCAACCC+TGG - chr4.1:75495755-75495774 MS.gene87542:intron 55.0%
! ACCGCGCCGCGTCAATTTTT+GGG - chr4.1:75495403-75495422 MS.gene87542:intron 55.0%
! TACCGCGCCGCGTCAATTTT+TGG - chr4.1:75495402-75495421 MS.gene87542:intron 55.0%
!! GTGGGGTCTTTTGGCTGATG+AGG - chr4.1:75496498-75496517 MS.gene87542:intron 55.0%
AATTGACGCGGCGCGGTATG+CGG + chr4.1:75495400-75495419 None:intergenic 60.0%
CAGCCAAAAGACCCCACCCT+CGG + chr4.1:75496495-75496514 None:intergenic 60.0%
CCCCAAAAATTGACGCGGCG+CGG + chr4.1:75495407-75495426 None:intergenic 60.0%
CCTGACAGTGCCTGTGGAGA+TGG - chr4.1:75495716-75495735 MS.gene87542:intron 60.0%
GCAAAGGTTCGCTGCCTCTG+TGG - chr4.1:75497156-75497175 MS.gene87542:CDS 60.0%
! CCATCTCCACAGGCACTGTC+AGG + chr4.1:75495719-75495738 None:intergenic 60.0%
! CCGCGCCGCGTCAATTTTTG+GGG - chr4.1:75495404-75495423 MS.gene87542:intron 60.0%
!! CGCCGCGTCAATTTTTGGGG+TGG - chr4.1:75495407-75495426 MS.gene87542:intron 60.0%
ATTGACGCGGCGCGGTATGC+GGG + chr4.1:75495399-75495418 None:intergenic 65.0%
GTCAAAGCGGAAACGCGGCG+CGG + chr4.1:75495433-75495452 None:intergenic 65.0%
! AGGCCGAGGGTGGGGTCTTT+TGG - chr4.1:75496489-75496508 MS.gene87542:intron 65.0%
! CGCGGTATGCGGGCGCGACG+CGG + chr4.1:75495389-75495408 None:intergenic 80.0%
! GCGGTATGCGGGCGCGACGC+GGG + chr4.1:75495388-75495407 None:intergenic 80.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 75494513 75497242 75494513 ID=MS.gene87542
chr4.1 mRNA 75494513 75497242 75494513 ID=MS.gene87542.t1;Parent=MS.gene87542
chr4.1 exon 75497082 75497242 75497082 ID=MS.gene87542.t1.exon1;Parent=MS.gene87542.t1
chr4.1 CDS 75497082 75497242 75497082 ID=cds.MS.gene87542.t1;Parent=MS.gene87542.t1
chr4.1 exon 75495944 75496100 75495944 ID=MS.gene87542.t1.exon2;Parent=MS.gene87542.t1
chr4.1 CDS 75495944 75496100 75495944 ID=cds.MS.gene87542.t1;Parent=MS.gene87542.t1
chr4.1 exon 75495253 75495312 75495253 ID=MS.gene87542.t1.exon3;Parent=MS.gene87542.t1
chr4.1 CDS 75495253 75495312 75495253 ID=cds.MS.gene87542.t1;Parent=MS.gene87542.t1
chr4.1 exon 75495039 75495123 75495039 ID=MS.gene87542.t1.exon4;Parent=MS.gene87542.t1
chr4.1 CDS 75495039 75495123 75495039 ID=cds.MS.gene87542.t1;Parent=MS.gene87542.t1
chr4.1 exon 75494865 75494958 75494865 ID=MS.gene87542.t1.exon5;Parent=MS.gene87542.t1
chr4.1 CDS 75494865 75494958 75494865 ID=cds.MS.gene87542.t1;Parent=MS.gene87542.t1
chr4.1 exon 75494513 75494726 75494513 ID=MS.gene87542.t1.exon6;Parent=MS.gene87542.t1
chr4.1 CDS 75494513 75494726 75494513 ID=cds.MS.gene87542.t1;Parent=MS.gene87542.t1
Gene Sequence

>MS.gene87542

ATGAACAACATTGTTATGTCTGCAGTTGAACTTATTTATTGTTATGCTGAATGCTTGGCCCTCCATGTAAAAGATTCAGGAGTGCACTGTGTAGCACCTGCTGTTGTATTACTAAAGAAACTTCTTTTTTCGTCTGATGAAGCTATTCAAACTGCTAGCAGGTGTGTTTTCTCACTGTTATTTTTTTTATTCTAACTTTGATAATCAGGTTTTTAGGGTTATAACTTAGAAGGCTATTGGGCCAAACATGTAAAGATGGGGCATGGGTCATATAAAGTTTCAAATAACCCAATACTCTTTTCCTAAAAATAATTAAAAAACCAATACCTACTAATGATTTTCTTACTAATGATACGTATTAAATATTTTAACTCTTTTTTTTATAAAAAATGTACATATTATTTATTTAATGTTTCTTTTTACAAGATTATTTATTTAATGTTGAAATATTTTAATGCATGTATTAAATACCGTTCTTTTTACTTATCTTTTTACTTAATATTAAATATATACTACTTATCTTTTTACTTAATATTAAATATATACTTATATTACTTAATATACAACTTTTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATCACAGTTCTTTTTTGAGATGCGAGTTGCAGGTGATATATTTCAAAAATTGCAGTTTTTTTATTCAATTATACGTAAGACTTTTTTTTTTGAAGGAAATTATACGTAAGACTTGTGGCTTTTATTTCAAATTATGAATAATTCATAAATTTTGTGTAATATTTAAGGTATTTTAATATATTTTTTGTATAGTATGGTGTTTTAATATTAAATATATTAGTGTCCGCGTCATGTAATAGCTCCCGCGTCGCGCCCGCATACCGCGCCGCGTCAATTTTTGGGGTGGCCGCGCCGCGTTTCCGCTTTGACAACTATGGTTTTACTTGAAATGTATTAGAAACTGATTTCTTGTTATATTTTGCCTTGCCTGTTGTGTATGATGCAGGGGCTAGGAATGTGACAAGAGCTGTGTCTTCAGAGGAATGGAAAATTGAATGACTATTGAATCACCATTCTTGAGAAATCTTAATGTTATATTAATGCATTTACACTTCTCTTTATCTCTCTGCAGGCATAAGAAGCAAAAGGAGGAGGATATTGCTATATGTGAATGCAGGTATGATGAAGATGATCCTGACAGTGCCTGTGGAGATGGGTGTCTAAATGTATTAACCAGCACTGAATGCAACCCTGGATTTTGCCCTTGTGGCATCCTTTGTAAAAATCAGGTGAGTTCGTGTTTGGGTCTGTTTGTGATAATATTTGTTAGTCTTTTGCAGTATAACCATCCTGCAGGTAGTTAAAAAGTTGGTCAAGAGAAGTTATTAGTTATTACTATATGCTTTAACATGTGTCTCTGAAGGCACTTGTCATAATTTGTTGCATATTTTTTTTGAGAGGATAATTTGTTGCATATTTGTATTCTGCTTTCCCTGGTTATTTTGTTCTACAAGTTTCTTAGTAGCTGTCAAGTTTGCTCGTCTTGAATTTTTTCATATCCTATTTTCATTGCATACTTTTTTTAATCTTTGACCTACTTAAACTTTACTTCAGATGATGCATTGAAGCTTACGTCAATTCATATATGATATATTCTGATATCGAAAAATGTTTTAGATGTTTCTAGTCAGTTAACTTAACTGGCATAACTGGCATTCAGTTATGAACGAACTAGTTAGTTAATAGCAGTCTGTAGTTACAATCGGTTACTCTGTACAACTTTTCTTCTAAGTAGTGCGGCTATAGATATCATTGCAATCGGAAGATGAATTCAATAGCTTTTACCTAAAGAAATATTCTCGTTTTCTTTCTCTCTTCTCTAGATGAAATATATTCATGTTCCATGTCTCATGCTTGCAGAAATTTCAGAAGTGTGAATATGCAAAAACAAAGTTATTTAAACTGAAGGCCGAGGGTGGGGTCTTTTGGCTGATGAGGAAATTAAGGTAATCATATAATGGGGAAATTTAGGCCTTAATATGGTTATTTTTCTTTTATTTCAGTAGTGCCATACGCTCATCTATTTATTTGTTTGTTTATTTCATACAGGCAGGACAGTTTGTCATTGAATACTTTGGAGAAGTAATATCATGCAAAGAAGCCAAGTGCAGATCCCATACTTATGAAACTCAAGGTCAAACATCAATAAAATTAATATTCTATCTGATGAGTATTTAGTTTATGTTTTTGTAAAACTTTTGTTTGCTTTTGCAGGTCTTAAGGATGCATTTATCATTTCTCTTAATGCGTATGAATCTATCGATGCAACTAGAAAAGGAAGCCTTGCAAGATTTATAAACCATTCCTGGTAAACTACTTCAAACAAAAATCCTAATATTAAATGAAATTTACCTGCCAATATGCAAGTATTGTAGACCTGTTTTGTTATCTTCCTATTTCCCACTGCCCAAGGTTTGATATCAACTGAATGCTTTTATGTTTGCAGTCAACCAAACTGTGAGACAAGAAAATGGAATGTCATGGGGGAAATAAGAGTTGGAATATTTGCACTGGAAGATATTCCTATTGGAACTGAACTGGCATATGACTATAATTTTGAATGGTTTGGAGGTGCAAAGGTTCGCTGCCTCTGTGGTGCGCTCAAATGTTCTGAATTCCTTGGAGCAAAATCTCGTGGTTTTCAAGTGAGTTTTAGTTAA
Protein sequence

>MS.gene87542.t1

MNNIVMSAVELIYCYAECLALHVKDSGVHCVAPAVVLLKKLLFSSDEAIQTASRHKKQKEEDIAICECRYDEDDPDSACGDGCLNVLTSTECNPGFCPCGILCKNQKFQKCEYAKTKLFKLKAEGGAGQFVIEYFGEVISCKEAKCRSHTYETQGLKDAFIISLNAYESIDATRKGSLARFINHSCQPNCETRKWNVMGEIRVGIFALEDIPIGTELAYDYNFEWFGGAKVRCLCGALKCSEFLGAKSRGFQVSFS