Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87648.t1 | XP_003601024.1 | 92.6 | 284 | 10 | 1 | 1 | 284 | 1 | 273 | 4.80E-145 | 523.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87648.t1 | Q9LYL9 | 47.3 | 275 | 143 | 2 | 1 | 275 | 1 | 273 | 6.5e-66 | 252.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87648.t1 | G7J2Y2 | 92.6 | 284 | 10 | 1 | 1 | 284 | 1 | 273 | 3.4e-145 | 523.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene87648.t1 | TR | TRAF |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87648.t1 | MTR_3g072160 | 92.606 | 284 | 10 | 1 | 1 | 284 | 1 | 273 | 0.0 | 520 |
| MS.gene87648.t1 | MTR_3g072160 | 94.954 | 218 | 11 | 0 | 67 | 284 | 19 | 236 | 2.46e-145 | 407 |
| MS.gene87648.t1 | MTR_3g088660 | 26.699 | 206 | 142 | 4 | 69 | 269 | 35 | 236 | 4.56e-19 | 84.7 |
| MS.gene87648.t1 | MTR_5g096700 | 31.847 | 157 | 103 | 3 | 88 | 244 | 83 | 235 | 2.36e-15 | 74.7 |
| MS.gene87648.t1 | MTR_5g096700 | 31.847 | 157 | 103 | 3 | 88 | 244 | 142 | 294 | 4.30e-15 | 74.7 |
| MS.gene87648.t1 | MTR_3g061090 | 31.548 | 168 | 107 | 4 | 77 | 244 | 62 | 221 | 2.02e-14 | 71.6 |
| MS.gene87648.t1 | MTR_3g061090 | 32.353 | 170 | 103 | 5 | 77 | 244 | 134 | 293 | 4.55e-14 | 71.6 |
| MS.gene87648.t1 | MTR_3g088560 | 30.539 | 167 | 105 | 5 | 80 | 244 | 10 | 167 | 4.23e-11 | 61.6 |
| MS.gene87648.t1 | MTR_1g029850 | 32.414 | 145 | 93 | 3 | 98 | 242 | 152 | 291 | 8.80e-11 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87648.t1 | AT3G56230 | 47.273 | 275 | 143 | 2 | 1 | 275 | 1 | 273 | 8.36e-85 | 256 |
| MS.gene87648.t1 | AT1G01640 | 46.632 | 193 | 97 | 4 | 88 | 279 | 1 | 188 | 8.86e-54 | 174 |
| MS.gene87648.t1 | AT1G01640 | 47.674 | 172 | 84 | 4 | 109 | 279 | 1 | 167 | 3.11e-47 | 156 |
| MS.gene87648.t1 | AT2G40450 | 40.000 | 180 | 103 | 3 | 96 | 270 | 9 | 188 | 1.38e-26 | 103 |
| MS.gene87648.t1 | AT5G48510 | 37.222 | 180 | 109 | 3 | 100 | 275 | 13 | 192 | 9.30e-26 | 102 |
| MS.gene87648.t1 | AT4G04090 | 32.353 | 170 | 114 | 1 | 106 | 274 | 20 | 189 | 4.10e-24 | 96.7 |
| MS.gene87648.t1 | AT2G05330 | 37.063 | 143 | 88 | 2 | 130 | 270 | 34 | 176 | 2.19e-21 | 90.1 |
| MS.gene87648.t1 | AT4G08455 | 28.230 | 209 | 134 | 6 | 73 | 269 | 44 | 248 | 5.49e-19 | 84.7 |
| MS.gene87648.t1 | AT2G05330 | 37.121 | 132 | 81 | 2 | 141 | 270 | 1 | 132 | 1.14e-17 | 79.0 |
| MS.gene87648.t1 | AT3G29740 | 44.186 | 86 | 44 | 2 | 89 | 171 | 3 | 87 | 2.12e-13 | 65.1 |
| MS.gene87648.t1 | AT1G21780 | 32.414 | 145 | 93 | 3 | 98 | 242 | 148 | 287 | 2.51e-12 | 66.6 |
| MS.gene87648.t1 | AT1G21780 | 32.414 | 145 | 93 | 3 | 98 | 242 | 148 | 287 | 2.51e-12 | 66.6 |
| MS.gene87648.t1 | AT1G55760 | 34.752 | 141 | 81 | 5 | 107 | 244 | 160 | 292 | 8.67e-11 | 62.0 |
Find 44 sgRNAs with CRISPR-Local
Find 65 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTGGAGGTCTTAATATTAA+TGG | 0.173482 | 3.1:+59073733 | None:intergenic |
| TTATAGAGAAATTGATAATT+TGG | 0.186682 | 3.1:+59072428 | None:intergenic |
| TGCACAAATGCTTCAAATTT+AGG | 0.193574 | 3.1:+59072504 | None:intergenic |
| ATTCTAATTCTTCATGGTTC+AGG | 0.311738 | 3.1:+59072781 | None:intergenic |
| TAGTGTCTCAAATGCATTAG+AGG | 0.315759 | 3.1:+59072614 | None:intergenic |
| CTCGAATTTCGCCGCGAAAT+AGG | 0.318434 | 3.1:+59073604 | None:intergenic |
| AGGATGTCTCCTTCAAATCT+TGG | 0.335688 | 3.1:+59072568 | None:intergenic |
| ACCTGCTCATAAATCCGTTT+TGG | 0.337217 | 3.1:-59073313 | MS.gene87648:intron |
| CTACAGTGGGACATTGCCTT+TGG | 0.341122 | 3.1:-59072742 | MS.gene87648:CDS |
| AGAGAGATTCTAATTCTTCA+TGG | 0.342558 | 3.1:+59072775 | None:intergenic |
| GCCCCACATATTGTATTCCT+TGG | 0.412240 | 3.1:+59073715 | None:intergenic |
| GGGCATGTTATGAAGGAGTT+AGG | 0.432141 | 3.1:-59073696 | MS.gene87648:CDS |
| TGTGTGAATTTGTTCTTTGA+AGG | 0.437509 | 3.1:+59073374 | None:intergenic |
| GTTTCAAGAGCATTTGTGAA+TGG | 0.440008 | 3.1:-59072453 | MS.gene87648:CDS |
| TTGTTCAAACCAAGATTTGA+AGG | 0.441270 | 3.1:-59072577 | MS.gene87648:CDS |
| ACGGATTTATGAGCAGGTAT+AGG | 0.452650 | 3.1:+59073318 | None:intergenic |
| CCACATATTGTATTCCTTGG+AGG | 0.461630 | 3.1:+59073718 | None:intergenic |
| TTGATAAGTCCAGGCAATCT+TGG | 0.471602 | 3.1:-59073345 | MS.gene87648:CDS |
| AAAACAATCACTACTCCAAA+TGG | 0.473612 | 3.1:-59073631 | MS.gene87648:CDS |
| AATTCTTCATGGTTCAGGTC+AGG | 0.475170 | 3.1:+59072786 | None:intergenic |
| AGAGTTATTGTGTTACAAGT+TGG | 0.476255 | 3.1:+59072810 | None:intergenic |
| ATAGGTGGACCAAGATTGCC+TGG | 0.498249 | 3.1:+59073336 | None:intergenic |
| TCACAGTATTTCAGCAAATG+TGG | 0.504898 | 3.1:+59072663 | None:intergenic |
| CACAGTATTTCAGCAAATGT+GGG | 0.507721 | 3.1:+59072664 | None:intergenic |
| AATTTGAAGCATTTGTGCAT+AGG | 0.515796 | 3.1:-59072500 | MS.gene87648:CDS |
| TTCGCGGCGAAATTCGAGTA+AGG | 0.516513 | 3.1:-59073599 | MS.gene87648:CDS |
| CCTCCAAGGAATACAATATG+TGG | 0.523672 | 3.1:-59073718 | MS.gene87648:CDS |
| ACCAAAACGGATTTATGAGC+AGG | 0.528361 | 3.1:+59073312 | None:intergenic |
| ATATGTGGGGCATGTTATGA+AGG | 0.533797 | 3.1:-59073703 | MS.gene87648:CDS |
| TCTTAGAATTTCTCTACAGT+GGG | 0.539455 | 3.1:-59072755 | MS.gene87648:CDS |
| CGCGAAATAGGAGAACCATT+TGG | 0.543566 | 3.1:+59073616 | None:intergenic |
| CAAGATTTGGCTTATCTCAG+AGG | 0.546377 | 3.1:-59073408 | MS.gene87648:CDS |
| CAAATGGTTCTCCTATTTCG+CGG | 0.549333 | 3.1:-59073615 | MS.gene87648:CDS |
| AAAACAAGAACTCACCAAAA+CGG | 0.553720 | 3.1:+59073299 | None:intergenic |
| ACAGATATATTGATAAGTCC+AGG | 0.558976 | 3.1:-59073354 | MS.gene87648:CDS |
| CTCTTAGAATTTCTCTACAG+TGG | 0.583649 | 3.1:-59072756 | MS.gene87648:CDS |
| CTCCAAGGAATACAATATGT+GGG | 0.586870 | 3.1:-59073717 | MS.gene87648:CDS |
| CTTATCAGCTGCTTGTGACA+AGG | 0.589102 | 3.1:+59072695 | None:intergenic |
| CACTTGATGATTGCATAAGA+TGG | 0.603274 | 3.1:-59073467 | MS.gene87648:CDS |
| GATTTATGAGCAGGTATAGG+TGG | 0.605249 | 3.1:+59073321 | None:intergenic |
| AGCATGATCAACATGATGAA+CGG | 0.624401 | 3.1:-59073673 | MS.gene87648:CDS |
| CATTAATATTAAGACCTCCA+AGG | 0.644737 | 3.1:-59073732 | MS.gene87648:CDS |
| TCCAAGGAATACAATATGTG+GGG | 0.668052 | 3.1:-59073716 | MS.gene87648:CDS |
| ACTAATTGCACAGTTAAATG+CGG | 0.678765 | 3.1:+59072474 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GTTAATGAAAATTATTTAAT+GGG | + | chr3.1:59072916-59072935 | None:intergenic | 10.0% |
| !!! | AGATAAAATAAAGTATTTTT+TGG | - | chr3.1:59073015-59073034 | MS.gene87648:intron | 10.0% |
| !!! | ATTTTTAACTAAGAAATTAA+AGG | + | chr3.1:59073643-59073662 | None:intergenic | 10.0% |
| !! | GGTTAATGAAAATTATTTAA+TGG | + | chr3.1:59072917-59072936 | None:intergenic | 15.0% |
| !! | TATTTATGTTATTGTAATGT+AGG | - | chr3.1:59073299-59073318 | MS.gene87648:intron | 15.0% |
| !!! | TAAAATAAAGTATTTTTTGG+CGG | - | chr3.1:59073018-59073037 | MS.gene87648:intron | 15.0% |
| !!! | TATTTTTTTGTTTACAAAGA+GGG | + | chr3.1:59073092-59073111 | None:intergenic | 15.0% |
| !!! | TTATAGAGAAATTGATAATT+TGG | + | chr3.1:59073763-59073782 | None:intergenic | 15.0% |
| !!! | TTATTTTTTTGTTTACAAAG+AGG | + | chr3.1:59073093-59073112 | None:intergenic | 15.0% |
| !! | AAAGCTTAAAAAAAATAAGC+TGG | - | chr3.1:59073170-59073189 | MS.gene87648:intron | 20.0% |
| !! | AGTTAAAAATATTGAGCATA+TGG | - | chr3.1:59073653-59073672 | MS.gene87648:CDS | 20.0% |
| !! | TAATCAACAAAAACAAGATT+TGG | - | chr3.1:59072767-59072786 | MS.gene87648:CDS | 20.0% |
| !!! | CTTGTTTTTGTTGATTAAAT+TGG | + | chr3.1:59072764-59072783 | None:intergenic | 20.0% |
| !!! | TTCTTTTCATAATTCTTCTA+TGG | - | chr3.1:59072622-59072641 | MS.gene87648:CDS | 20.0% |
| ! | TCAAGTGTATAATCTAAAAG+AGG | - | chr3.1:59072668-59072687 | MS.gene87648:CDS | 25.0% |
| AAAACAAGAACTCACCAAAA+CGG | + | chr3.1:59072892-59072911 | None:intergenic | 30.0% | |
| AAAACAATCACTACTCCAAA+TGG | - | chr3.1:59072557-59072576 | MS.gene87648:CDS | 30.0% | |
| ACAGATATATTGATAAGTCC+AGG | - | chr3.1:59072834-59072853 | MS.gene87648:CDS | 30.0% | |
| ACTAATTGCACAGTTAAATG+CGG | + | chr3.1:59073717-59073736 | None:intergenic | 30.0% | |
| AGAGAGATTCTAATTCTTCA+TGG | + | chr3.1:59073416-59073435 | None:intergenic | 30.0% | |
| AGAGTTATTGTGTTACAAGT+TGG | + | chr3.1:59073381-59073400 | None:intergenic | 30.0% | |
| ATTCTAATTCTTCATGGTTC+AGG | + | chr3.1:59073410-59073429 | None:intergenic | 30.0% | |
| CATTAATATTAAGACCTCCA+AGG | - | chr3.1:59072456-59072475 | MS.gene87648:CDS | 30.0% | |
| CTTGGAGGTCTTAATATTAA+TGG | + | chr3.1:59072458-59072477 | None:intergenic | 30.0% | |
| TCTTAGAATTTCTCTACAGT+GGG | - | chr3.1:59073433-59073452 | MS.gene87648:CDS | 30.0% | |
| TGCACAAATGCTTCAAATTT+AGG | + | chr3.1:59073687-59073706 | None:intergenic | 30.0% | |
| TTGTTCAAACCAAGATTTGA+AGG | - | chr3.1:59073611-59073630 | MS.gene87648:CDS | 30.0% | |
| ! | AATTTGAAGCATTTGTGCAT+AGG | - | chr3.1:59073688-59073707 | MS.gene87648:CDS | 30.0% |
| ! | TGTGTGAATTTGTTCTTTGA+AGG | + | chr3.1:59072817-59072836 | None:intergenic | 30.0% |
| ! | TGTTTCTCCAATTTTTCCAA+AGG | + | chr3.1:59073465-59073484 | None:intergenic | 30.0% |
| !!! | ATCTCGGTGTAGTTTTTTTT+TGG | - | chr3.1:59073058-59073077 | MS.gene87648:intron | 30.0% |
| !!! | TCTCGGTGTAGTTTTTTTTT+GGG | - | chr3.1:59073059-59073078 | MS.gene87648:intron | 30.0% |
| AGCATGATCAACATGATGAA+CGG | - | chr3.1:59072515-59072534 | MS.gene87648:CDS | 35.0% | |
| CACAGTATTTCAGCAAATGT+GGG | + | chr3.1:59073527-59073546 | None:intergenic | 35.0% | |
| CTCCAAGGAATACAATATGT+GGG | - | chr3.1:59072471-59072490 | MS.gene87648:CDS | 35.0% | |
| CTCTTAGAATTTCTCTACAG+TGG | - | chr3.1:59073432-59073451 | MS.gene87648:CDS | 35.0% | |
| TAGTGTCTCAAATGCATTAG+AGG | + | chr3.1:59073577-59073596 | None:intergenic | 35.0% | |
| TCACAGTATTTCAGCAAATG+TGG | + | chr3.1:59073528-59073547 | None:intergenic | 35.0% | |
| TCCAAGGAATACAATATGTG+GGG | - | chr3.1:59072472-59072491 | MS.gene87648:CDS | 35.0% | |
| ! | CACTTGATGATTGCATAAGA+TGG | - | chr3.1:59072721-59072740 | MS.gene87648:CDS | 35.0% |
| ! | GACATTGCCTTTGGAAAAAT+TGG | - | chr3.1:59073455-59073474 | MS.gene87648:CDS | 35.0% |
| !! | GTTTCAAGAGCATTTGTGAA+TGG | - | chr3.1:59073735-59073754 | MS.gene87648:CDS | 35.0% |
| AATTCTTCATGGTTCAGGTC+AGG | + | chr3.1:59073405-59073424 | None:intergenic | 40.0% | |
| ACGGATTTATGAGCAGGTAT+AGG | + | chr3.1:59072873-59072892 | None:intergenic | 40.0% | |
| AGGATGTCTCCTTCAAATCT+TGG | + | chr3.1:59073623-59073642 | None:intergenic | 40.0% | |
| ATATGTGGGGCATGTTATGA+AGG | - | chr3.1:59072485-59072504 | MS.gene87648:CDS | 40.0% | |
| CAAATGGTTCTCCTATTTCG+CGG | - | chr3.1:59072573-59072592 | MS.gene87648:CDS | 40.0% | |
| CCACATATTGTATTCCTTGG+AGG | + | chr3.1:59072473-59072492 | None:intergenic | 40.0% | |
| CCTCCAAGGAATACAATATG+TGG | - | chr3.1:59072470-59072489 | MS.gene87648:CDS | 40.0% | |
| GATTTATGAGCAGGTATAGG+TGG | + | chr3.1:59072870-59072889 | None:intergenic | 40.0% | |
| TTCTGTCTCACGATTGATCT+CGG | - | chr3.1:59073042-59073061 | MS.gene87648:intron | 40.0% | |
| TTGATAAGTCCAGGCAATCT+TGG | - | chr3.1:59072843-59072862 | MS.gene87648:CDS | 40.0% | |
| ! | ACCAAAACGGATTTATGAGC+AGG | + | chr3.1:59072879-59072898 | None:intergenic | 40.0% |
| ! | ACCTGCTCATAAATCCGTTT+TGG | - | chr3.1:59072875-59072894 | MS.gene87648:intron | 40.0% |
| ! | CAAGATTTGGCTTATCTCAG+AGG | - | chr3.1:59072780-59072799 | MS.gene87648:CDS | 40.0% |
| CAATCATCAAGTGTCTGCAG+AGG | + | chr3.1:59072714-59072733 | None:intergenic | 45.0% | |
| CGCGAAATAGGAGAACCATT+TGG | + | chr3.1:59072575-59072594 | None:intergenic | 45.0% | |
| CTTATCAGCTGCTTGTGACA+AGG | + | chr3.1:59073496-59073515 | None:intergenic | 45.0% | |
| GCCCCACATATTGTATTCCT+TGG | + | chr3.1:59072476-59072495 | None:intergenic | 45.0% | |
| GGGCATGTTATGAAGGAGTT+AGG | - | chr3.1:59072492-59072511 | MS.gene87648:CDS | 45.0% | |
| !!! | AAATTTTAAATATTAAAGAA+TGG | + | chr3.1:59072938-59072957 | None:intergenic | 5.0% |
| ATAGGTGGACCAAGATTGCC+TGG | + | chr3.1:59072855-59072874 | None:intergenic | 50.0% | |
| CTACAGTGGGACATTGCCTT+TGG | - | chr3.1:59073446-59073465 | MS.gene87648:CDS | 50.0% | |
| CTCGAATTTCGCCGCGAAAT+AGG | + | chr3.1:59072587-59072606 | None:intergenic | 50.0% | |
| TTCGCGGCGAAATTCGAGTA+AGG | - | chr3.1:59072589-59072608 | MS.gene87648:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 59072428 | 59073782 | 59072428 | ID=MS.gene87648 |
| chr3.1 | mRNA | 59072428 | 59073782 | 59072428 | ID=MS.gene87648.t1;Parent=MS.gene87648 |
| chr3.1 | exon | 59073567 | 59073782 | 59073567 | ID=MS.gene87648.t1.exon1;Parent=MS.gene87648.t1 |
| chr3.1 | CDS | 59073567 | 59073782 | 59073567 | ID=cds.MS.gene87648.t1;Parent=MS.gene87648.t1 |
| chr3.1 | exon | 59073314 | 59073490 | 59073314 | ID=MS.gene87648.t1.exon2;Parent=MS.gene87648.t1 |
| chr3.1 | CDS | 59073314 | 59073490 | 59073314 | ID=cds.MS.gene87648.t1;Parent=MS.gene87648.t1 |
| chr3.1 | exon | 59072428 | 59072889 | 59072428 | ID=MS.gene87648.t1.exon3;Parent=MS.gene87648.t1 |
| chr3.1 | CDS | 59072428 | 59072889 | 59072428 | ID=cds.MS.gene87648.t1;Parent=MS.gene87648.t1 |
| Gene Sequence |
| Protein sequence |
