Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87703.t1 | AES59479.2 | 91.3 | 127 | 11 | 0 | 1 | 127 | 33 | 159 | 1.40E-57 | 232.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87703.t1 | G7I4G2 | 91.3 | 127 | 11 | 0 | 1 | 127 | 33 | 159 | 9.8e-58 | 232.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene87703.t1 | TF | FAR1 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87703.t1 | MTR_1g019850 | 91.339 | 127 | 11 | 0 | 1 | 127 | 33 | 159 | 4.73e-81 | 238 |
| MS.gene87703.t1 | MTR_2g024230 | 77.419 | 93 | 20 | 1 | 3 | 94 | 54 | 146 | 7.61e-45 | 144 |
| MS.gene87703.t1 | MTR_0853s0010 | 50.847 | 118 | 43 | 2 | 25 | 127 | 4 | 121 | 1.17e-31 | 110 |
| MS.gene87703.t1 | MTR_1g033820 | 52.000 | 50 | 24 | 0 | 33 | 82 | 22 | 71 | 3.74e-14 | 63.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 20 sgRNAs with CRISPR-Local
Find 23 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACTCCAAAACCTTCTTTCT+TGG | 0.206214 | 7.1:+29082353 | None:intergenic |
| TATTTGTCCTATTGTTGATT+TGG | 0.256460 | 7.1:-29082421 | MS.gene87703:CDS |
| AAGCAGGGAAGAGAAGTAAA+TGG | 0.304863 | 7.1:-29082188 | MS.gene87703:CDS |
| TTTGCCAAGAAAGAAGGTTT+TGG | 0.354079 | 7.1:-29082357 | MS.gene87703:CDS |
| TCAAATTCCAAATCAACAAT+AGG | 0.420244 | 7.1:+29082414 | None:intergenic |
| GAAGGTACACATATAGTAAA+GGG | 0.439216 | 7.1:-29082282 | MS.gene87703:CDS |
| TCACTTACTGTTTCAAAAGC+AGG | 0.452498 | 7.1:-29082204 | MS.gene87703:CDS |
| ACATCGTTTGCCAAGAAAGA+AGG | 0.462978 | 7.1:-29082363 | MS.gene87703:CDS |
| ATAGGACAAATATTAGCTTC+AGG | 0.470032 | 7.1:+29082432 | None:intergenic |
| AGCAGGGAAGAGAAGTAAAT+GGG | 0.489503 | 7.1:-29082187 | MS.gene87703:CDS |
| TGAAGGTACACATATAGTAA+AGG | 0.494494 | 7.1:-29082283 | MS.gene87703:CDS |
| AATATTAGCTTCAGGCGACT+TGG | 0.496239 | 7.1:+29082440 | None:intergenic |
| GTAAAGGGTGAAAATGAAGA+AGG | 0.507431 | 7.1:-29082267 | MS.gene87703:CDS |
| TGCTTTACAATCAGTTCTTG+AGG | 0.537334 | 7.1:+29082227 | None:intergenic |
| CACTTACTGTTTCAAAAGCA+GGG | 0.542698 | 7.1:-29082203 | MS.gene87703:CDS |
| TAAAGGGTGAAAATGAAGAA+GGG | 0.554329 | 7.1:-29082266 | MS.gene87703:CDS |
| ATGTTTGTACGCGCTAATGA+AGG | 0.560428 | 7.1:-29082300 | MS.gene87703:CDS |
| ACACATCATCAACTATCTCA+TGG | 0.582009 | 7.1:+29082493 | None:intergenic |
| GTCTTCTAAACACTCAACAG+TGG | 0.678815 | 7.1:+29082515 | None:intergenic |
| TTTGGAATTTGACTCCATTG+CGG | 0.690541 | 7.1:-29082403 | MS.gene87703:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TCAAATTCCAAATCAACAAT+AGG | + | chr7.1:29082257-29082276 | None:intergenic | 25.0% |
| !! | TATTTGTCCTATTGTTGATT+TGG | - | chr7.1:29082247-29082266 | MS.gene87703:CDS | 25.0% |
| TAAAGGGTGAAAATGAAGAA+GGG | - | chr7.1:29082402-29082421 | MS.gene87703:CDS | 30.0% | |
| ! | ATAGGACAAATATTAGCTTC+AGG | + | chr7.1:29082239-29082258 | None:intergenic | 30.0% |
| ! | GAAGGTACACATATAGTAAA+GGG | - | chr7.1:29082386-29082405 | MS.gene87703:CDS | 30.0% |
| ! | GACTTGGAATTTTCTGAATT+TGG | + | chr7.1:29082215-29082234 | None:intergenic | 30.0% |
| ! | TGAAGGTACACATATAGTAA+AGG | - | chr7.1:29082385-29082404 | MS.gene87703:CDS | 30.0% |
| AACTCCAAAACCTTCTTTCT+TGG | + | chr7.1:29082318-29082337 | None:intergenic | 35.0% | |
| ACACATCATCAACTATCTCA+TGG | + | chr7.1:29082178-29082197 | None:intergenic | 35.0% | |
| CACTTACTGTTTCAAAAGCA+GGG | - | chr7.1:29082465-29082484 | MS.gene87703:CDS | 35.0% | |
| GTAAAGGGTGAAAATGAAGA+AGG | - | chr7.1:29082401-29082420 | MS.gene87703:CDS | 35.0% | |
| TCACTTACTGTTTCAAAAGC+AGG | - | chr7.1:29082464-29082483 | MS.gene87703:CDS | 35.0% | |
| TGCTTTACAATCAGTTCTTG+AGG | + | chr7.1:29082444-29082463 | None:intergenic | 35.0% | |
| ! | GAATTCTTTTACATCCGCAA+TGG | + | chr7.1:29082282-29082301 | None:intergenic | 35.0% |
| ! | TTTGCCAAGAAAGAAGGTTT+TGG | - | chr7.1:29082311-29082330 | MS.gene87703:CDS | 35.0% |
| ! | TTTGGAATTTGACTCCATTG+CGG | - | chr7.1:29082265-29082284 | MS.gene87703:CDS | 35.0% |
| !!! | AAGGTTTTGGAGTTCTTGTT+CGG | - | chr7.1:29082324-29082343 | MS.gene87703:CDS | 35.0% |
| AAGCAGGGAAGAGAAGTAAA+TGG | - | chr7.1:29082480-29082499 | MS.gene87703:CDS | 40.0% | |
| ACATCGTTTGCCAAGAAAGA+AGG | - | chr7.1:29082305-29082324 | MS.gene87703:CDS | 40.0% | |
| AGCAGGGAAGAGAAGTAAAT+GGG | - | chr7.1:29082481-29082500 | MS.gene87703:CDS | 40.0% | |
| ATGTTTGTACGCGCTAATGA+AGG | - | chr7.1:29082368-29082387 | MS.gene87703:CDS | 40.0% | |
| GTCTTCTAAACACTCAACAG+TGG | + | chr7.1:29082156-29082175 | None:intergenic | 40.0% | |
| ! | AATATTAGCTTCAGGCGACT+TGG | + | chr7.1:29082231-29082250 | None:intergenic | 40.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 29082143 | 29082547 | 29082143 | ID=MS.gene87703 |
| chr7.1 | mRNA | 29082143 | 29082547 | 29082143 | ID=MS.gene87703.t1;Parent=MS.gene87703 |
| chr7.1 | exon | 29082143 | 29082547 | 29082143 | ID=MS.gene87703.t1.exon1;Parent=MS.gene87703.t1 |
| chr7.1 | CDS | 29082143 | 29082547 | 29082143 | ID=cds.MS.gene87703.t1;Parent=MS.gene87703.t1 |
| Gene Sequence |
| Protein sequence |
