Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88005.t1 | XP_013441754.1 | 87.5 | 256 | 31 | 1 | 1 | 255 | 1 | 256 | 2.30E-122 | 448.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88005.t1 | A0A072TE13 | 87.5 | 256 | 31 | 1 | 1 | 255 | 1 | 256 | 1.7e-122 | 448.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene88005.t1 | TF | FAR1 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88005.t1 | MTR_0558s0020 | 87.500 | 256 | 31 | 1 | 1 | 255 | 1 | 256 | 1.47e-167 | 464 |
| MS.gene88005.t1 | MTR_1g083640 | 81.919 | 271 | 33 | 1 | 1 | 255 | 1 | 271 | 8.70e-166 | 460 |
| MS.gene88005.t1 | MTR_2g014100 | 81.633 | 245 | 36 | 1 | 1 | 236 | 1 | 245 | 1.54e-147 | 412 |
| MS.gene88005.t1 | MTR_8g446020 | 71.765 | 255 | 33 | 1 | 1 | 255 | 1 | 216 | 2.52e-128 | 364 |
| MS.gene88005.t1 | MTR_0337s0040 | 71.875 | 256 | 32 | 2 | 1 | 255 | 1 | 217 | 1.56e-126 | 358 |
| MS.gene88005.t1 | MTR_3g008240 | 84.236 | 203 | 31 | 1 | 1 | 202 | 1 | 203 | 1.55e-124 | 352 |
| MS.gene88005.t1 | MTR_4g040430 | 82.178 | 202 | 32 | 1 | 1 | 202 | 1 | 198 | 8.69e-120 | 340 |
| MS.gene88005.t1 | MTR_6g090505 | 84.293 | 191 | 30 | 0 | 65 | 255 | 1 | 191 | 4.89e-118 | 342 |
| MS.gene88005.t1 | MTR_5g071590 | 73.333 | 180 | 35 | 2 | 1 | 167 | 1 | 180 | 1.60e-92 | 271 |
| MS.gene88005.t1 | MTR_1g055240 | 86.620 | 142 | 19 | 0 | 91 | 232 | 3 | 144 | 5.30e-90 | 263 |
| MS.gene88005.t1 | MTR_0019s0290 | 80.921 | 152 | 29 | 0 | 82 | 233 | 9 | 160 | 5.08e-89 | 261 |
| MS.gene88005.t1 | MTR_1281s0010 | 78.899 | 109 | 23 | 0 | 19 | 127 | 1 | 109 | 4.13e-58 | 181 |
| MS.gene88005.t1 | MTR_4g008820 | 73.958 | 96 | 24 | 1 | 1 | 95 | 125 | 220 | 1.73e-35 | 134 |
| MS.gene88005.t1 | MTR_8g046060 | 78.409 | 88 | 10 | 2 | 1 | 79 | 91 | 178 | 1.58e-34 | 124 |
| MS.gene88005.t1 | MTR_6g051425 | 28.241 | 216 | 136 | 5 | 40 | 253 | 22 | 220 | 3.93e-17 | 79.7 |
| MS.gene88005.t1 | MTR_0402s0040 | 24.865 | 185 | 120 | 4 | 49 | 233 | 40 | 205 | 8.25e-15 | 73.9 |
| MS.gene88005.t1 | MTR_1g013060 | 30.415 | 217 | 130 | 8 | 40 | 253 | 29 | 227 | 2.05e-13 | 68.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88005.t1 | AT4G38180 | 25.909 | 220 | 146 | 4 | 32 | 251 | 70 | 272 | 4.35e-13 | 68.9 |
Find 37 sgRNAs with CRISPR-Local
Find 45 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTAAGCAGACTTCCCTTCT+TGG | 0.303698 | 4.3:+70097777 | None:intergenic |
| TTCTCATACTCAACAAGAAC+TGG | 0.395633 | 4.3:+70097727 | None:intergenic |
| CCATGATTCTTTATCACTAA+AGG | 0.406174 | 4.3:+70097966 | None:intergenic |
| TAAAGAATCATGGAAGTCTT+TGG | 0.408500 | 4.3:-70097956 | MS.gene88005:CDS |
| ATAACACTGGATTCTCTGTT+AGG | 0.417808 | 4.3:-70097885 | MS.gene88005:CDS |
| CGAGAAGTAAATGAAGTTCA+AGG | 0.430014 | 4.3:-70097550 | MS.gene88005:CDS |
| AGCTGGAATATATTTGAAGT+TGG | 0.433136 | 4.3:-70097668 | MS.gene88005:CDS |
| TGAATGTCTATAAGATATAT+AGG | 0.445219 | 4.3:-70097636 | MS.gene88005:CDS |
| GAAAGGGTTGGTTGTAAAGC+TGG | 0.447509 | 4.3:-70097685 | MS.gene88005:CDS |
| GCTTTAATAAATTCTAAAGC+TGG | 0.450264 | 4.3:-70097520 | MS.gene88005:CDS |
| CTCAAGTAGCGCAAATGCTC+TGG | 0.460440 | 4.3:+70097580 | None:intergenic |
| ACAAAATAAGAAATCGGATA+AGG | 0.469178 | 4.3:-70097836 | MS.gene88005:CDS |
| TTACAACCAACCCTTTCCTC+TGG | 0.485330 | 4.3:+70097691 | None:intergenic |
| TTTAATTATGCATATAACAC+TGG | 0.485542 | 4.3:-70097898 | MS.gene88005:CDS |
| TCTAATGACGACTCGTCAAA+AGG | 0.488508 | 4.3:-70097407 | MS.gene88005:CDS |
| CAATTCACAAAATAAGAAAT+CGG | 0.490263 | 4.3:-70097842 | MS.gene88005:CDS |
| CGTCAAAAGGAGATGGTGGC+TGG | 0.492100 | 4.3:-70097394 | MS.gene88005:CDS |
| TGTCTTATGCAGCATCAACT+TGG | 0.501386 | 4.3:+70097302 | None:intergenic |
| TTTAAGTCCGAAAATCGAAA+AGG | 0.508080 | 4.3:+70097436 | None:intergenic |
| TGTCTATAAGATATATAGGT+GGG | 0.523740 | 4.3:-70097632 | MS.gene88005:CDS |
| CCTTTAGTGATAAAGAATCA+TGG | 0.543522 | 4.3:-70097966 | MS.gene88005:CDS |
| AACAAAGAAGGTTCCAAGAA+GGG | 0.550046 | 4.3:-70097790 | MS.gene88005:CDS |
| GAGAACATCTTATGAAGTGA+TGG | 0.551307 | 4.3:-70097488 | MS.gene88005:CDS |
| AAATGCTCTGGTTTATGTAA+TGG | 0.552634 | 4.3:+70097592 | None:intergenic |
| TGAAAATTCAGTTATTGATG+CGG | 0.559614 | 4.3:-70098007 | MS.gene88005:CDS |
| GGGTGAAGGTGTTGGAGGCA+CGG | 0.565649 | 4.3:-70097467 | MS.gene88005:CDS |
| TGTTATGCTTGTAACAAAGA+AGG | 0.570621 | 4.3:-70097802 | MS.gene88005:CDS |
| TAACAAAGAAGGTTCCAAGA+AGG | 0.572559 | 4.3:-70097791 | MS.gene88005:CDS |
| GAAGTGATGGGTGAAGGTGT+TGG | 0.603704 | 4.3:-70097475 | MS.gene88005:CDS |
| GACGACTCGTCAAAAGGAGA+TGG | 0.604288 | 4.3:-70097401 | MS.gene88005:CDS |
| GACTCGTCAAAAGGAGATGG+TGG | 0.611157 | 4.3:-70097398 | MS.gene88005:CDS |
| ATATCAACTTCATCTGAGAG+TGG | 0.614980 | 4.3:-70098042 | MS.gene88005:CDS |
| AGAACTGGACTGTCACATGT+TGG | 0.620791 | 4.3:+70097742 | None:intergenic |
| GTGATGGGTGAAGGTGTTGG+AGG | 0.621481 | 4.3:-70097472 | MS.gene88005:CDS |
| AGAACATCTTATGAAGTGAT+GGG | 0.643044 | 4.3:-70097487 | MS.gene88005:CDS |
| ATGTCTATAAGATATATAGG+TGG | 0.643489 | 4.3:-70097633 | MS.gene88005:CDS |
| TCTTATGAAGTGATGGGTGA+AGG | 0.656961 | 4.3:-70097481 | MS.gene88005:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CAATTCACAAAATAAGAAAT+CGG | - | chr4.3:70097515-70097534 | MS.gene88005:CDS | 20.0% |
| !! | TGAATGTCTATAAGATATAT+AGG | - | chr4.3:70097721-70097740 | MS.gene88005:CDS | 20.0% |
| !! | TTTAATTATGCATATAACAC+TGG | - | chr4.3:70097459-70097478 | MS.gene88005:CDS | 20.0% |
| ! | ACAAAATAAGAAATCGGATA+AGG | - | chr4.3:70097521-70097540 | MS.gene88005:CDS | 25.0% |
| ! | ATGTCTATAAGATATATAGG+TGG | - | chr4.3:70097724-70097743 | MS.gene88005:CDS | 25.0% |
| ! | TGAAAATTCAGTTATTGATG+CGG | - | chr4.3:70097350-70097369 | MS.gene88005:CDS | 25.0% |
| ! | TGGATATCATAGTAAAAACA+TGG | + | chr4.3:70098038-70098057 | None:intergenic | 25.0% |
| ! | TGTCTATAAGATATATAGGT+GGG | - | chr4.3:70097725-70097744 | MS.gene88005:CDS | 25.0% |
| !!! | CTTATTTTGTGAATTGTTCT+TGG | + | chr4.3:70097511-70097530 | None:intergenic | 25.0% |
| !!! | GCTTTAATAAATTCTAAAGC+TGG | - | chr4.3:70097837-70097856 | MS.gene88005:CDS | 25.0% |
| AGAACATCTTATGAAGTGAT+GGG | - | chr4.3:70097870-70097889 | MS.gene88005:CDS | 30.0% | |
| CCATGATTCTTTATCACTAA+AGG | + | chr4.3:70097394-70097413 | None:intergenic | 30.0% | |
| CCTTTAGTGATAAAGAATCA+TGG | - | chr4.3:70097391-70097410 | MS.gene88005:CDS | 30.0% | |
| TAAAGAAAAACCAGAGGAAA+GGG | - | chr4.3:70097656-70097675 | MS.gene88005:CDS | 30.0% | |
| TAAAGAATCATGGAAGTCTT+TGG | - | chr4.3:70097401-70097420 | MS.gene88005:CDS | 30.0% | |
| TGTTATGCTTGTAACAAAGA+AGG | - | chr4.3:70097555-70097574 | MS.gene88005:CDS | 30.0% | |
| TTTAAGTCCGAAAATCGAAA+AGG | + | chr4.3:70097924-70097943 | None:intergenic | 30.0% | |
| ! | AAATGCTCTGGTTTATGTAA+TGG | + | chr4.3:70097768-70097787 | None:intergenic | 30.0% |
| ! | AGCTGGAATATATTTGAAGT+TGG | - | chr4.3:70097689-70097708 | MS.gene88005:CDS | 30.0% |
| ! | GAATCTTCCTTTTCGATTTT+CGG | - | chr4.3:70097914-70097933 | MS.gene88005:CDS | 30.0% |
| AACAAAGAAGGTTCCAAGAA+GGG | - | chr4.3:70097567-70097586 | MS.gene88005:CDS | 35.0% | |
| ATAACACTGGATTCTCTGTT+AGG | - | chr4.3:70097472-70097491 | MS.gene88005:CDS | 35.0% | |
| ATATCAACTTCATCTGAGAG+TGG | - | chr4.3:70097315-70097334 | MS.gene88005:CDS | 35.0% | |
| CGAGAAGTAAATGAAGTTCA+AGG | - | chr4.3:70097807-70097826 | MS.gene88005:CDS | 35.0% | |
| CTAAAGAAAAACCAGAGGAA+AGG | - | chr4.3:70097655-70097674 | MS.gene88005:CDS | 35.0% | |
| GAAAGCTAAAGAAAAACCAG+AGG | - | chr4.3:70097650-70097669 | MS.gene88005:CDS | 35.0% | |
| GAGAACATCTTATGAAGTGA+TGG | - | chr4.3:70097869-70097888 | MS.gene88005:CDS | 35.0% | |
| GTCTGCTTAATCCTAAAAAC+AGG | - | chr4.3:70097592-70097611 | MS.gene88005:CDS | 35.0% | |
| TAACAAAGAAGGTTCCAAGA+AGG | - | chr4.3:70097566-70097585 | MS.gene88005:CDS | 35.0% | |
| TTCTCATACTCAACAAGAAC+TGG | + | chr4.3:70097633-70097652 | None:intergenic | 35.0% | |
| ATTAAGCAGACTTCCCTTCT+TGG | + | chr4.3:70097583-70097602 | None:intergenic | 40.0% | |
| TCTAATGACGACTCGTCAAA+AGG | - | chr4.3:70097950-70097969 | MS.gene88005:CDS | 40.0% | |
| !! | TCACATGTTGGCCTGTTTTT+AGG | + | chr4.3:70097606-70097625 | None:intergenic | 40.0% |
| !! | TCTTATGAAGTGATGGGTGA+AGG | - | chr4.3:70097876-70097895 | MS.gene88005:CDS | 40.0% |
| AGAACTGGACTGTCACATGT+TGG | + | chr4.3:70097618-70097637 | None:intergenic | 45.0% | |
| GAAAAACCAGAGGAAAGGGT+TGG | - | chr4.3:70097660-70097679 | MS.gene88005:CDS | 45.0% | |
| TTACAACCAACCCTTTCCTC+TGG | + | chr4.3:70097669-70097688 | None:intergenic | 45.0% | |
| !! | GAAAGGGTTGGTTGTAAAGC+TGG | - | chr4.3:70097672-70097691 | MS.gene88005:CDS | 45.0% |
| CTCAAGTAGCGCAAATGCTC+TGG | + | chr4.3:70097780-70097799 | None:intergenic | 50.0% | |
| GACGACTCGTCAAAAGGAGA+TGG | - | chr4.3:70097956-70097975 | MS.gene88005:CDS | 50.0% | |
| GACTCGTCAAAAGGAGATGG+TGG | - | chr4.3:70097959-70097978 | MS.gene88005:CDS | 50.0% | |
| !! | GAAGTGATGGGTGAAGGTGT+TGG | - | chr4.3:70097882-70097901 | MS.gene88005:CDS | 50.0% |
| ! | CGTCAAAAGGAGATGGTGGC+TGG | - | chr4.3:70097963-70097982 | MS.gene88005:CDS | 55.0% |
| !! | GTGATGGGTGAAGGTGTTGG+AGG | - | chr4.3:70097885-70097904 | MS.gene88005:CDS | 55.0% |
| !! | GGGTGAAGGTGTTGGAGGCA+CGG | - | chr4.3:70097890-70097909 | MS.gene88005:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 70097306 | 70098073 | 70097306 | ID=MS.gene88005 |
| chr4.3 | mRNA | 70097306 | 70098073 | 70097306 | ID=MS.gene88005.t1;Parent=MS.gene88005 |
| chr4.3 | exon | 70097306 | 70098073 | 70097306 | ID=MS.gene88005.t1.exon1;Parent=MS.gene88005.t1 |
| chr4.3 | CDS | 70097306 | 70098073 | 70097306 | ID=cds.MS.gene88005.t1;Parent=MS.gene88005.t1 |
| Gene Sequence |
| Protein sequence |
