Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88099.t1 | XP_003605494.2 | 81 | 242 | 33 | 1 | 4 | 245 | 3 | 231 | 5.60E-102 | 380.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88099.t1 | Q9FJK3 | 47.7 | 193 | 99 | 2 | 4 | 196 | 3 | 193 | 1.3e-38 | 161.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88099.t1 | G7JN54 | 81.0 | 242 | 33 | 1 | 4 | 245 | 3 | 231 | 4.1e-102 | 380.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene88099.t1 | TF | MADS-M-type |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88099.t1 | MTR_4g031910 | 83.471 | 242 | 25 | 1 | 4 | 245 | 3 | 229 | 1.31e-138 | 388 |
| MS.gene88099.t1 | MTR_4g032290 | 80.992 | 242 | 33 | 1 | 4 | 245 | 3 | 231 | 6.16e-133 | 374 |
| MS.gene88099.t1 | MTR_8g036130 | 61.753 | 251 | 81 | 5 | 4 | 245 | 3 | 247 | 1.02e-98 | 288 |
| MS.gene88099.t1 | MTR_4g032620 | 58.367 | 245 | 88 | 4 | 1 | 245 | 1 | 231 | 1.40e-94 | 277 |
| MS.gene88099.t1 | MTR_4g032260 | 81.818 | 154 | 28 | 0 | 4 | 157 | 3 | 156 | 4.74e-82 | 243 |
| MS.gene88099.t1 | MTR_3g065100 | 48.548 | 241 | 117 | 4 | 4 | 242 | 3 | 238 | 1.86e-64 | 201 |
| MS.gene88099.t1 | MTR_3g466980 | 54.902 | 153 | 68 | 1 | 4 | 156 | 3 | 154 | 8.85e-59 | 186 |
| MS.gene88099.t1 | MTR_4g032270 | 57.616 | 151 | 43 | 2 | 95 | 245 | 2 | 131 | 3.42e-53 | 168 |
| MS.gene88099.t1 | MTR_2g035610 | 73.333 | 120 | 32 | 0 | 4 | 123 | 3 | 122 | 1.34e-50 | 161 |
| MS.gene88099.t1 | MTR_3g466830 | 44.720 | 161 | 88 | 1 | 4 | 164 | 2 | 161 | 2.04e-44 | 147 |
| MS.gene88099.t1 | MTR_5g075380 | 45.333 | 150 | 81 | 1 | 6 | 155 | 5 | 153 | 1.25e-42 | 142 |
| MS.gene88099.t1 | MTR_4g063790 | 41.104 | 163 | 96 | 0 | 4 | 166 | 3 | 165 | 3.86e-42 | 141 |
| MS.gene88099.t1 | MTR_3g466890 | 42.138 | 159 | 91 | 1 | 4 | 162 | 2 | 159 | 6.02e-40 | 135 |
| MS.gene88099.t1 | MTR_3g031100 | 42.038 | 157 | 90 | 1 | 1 | 157 | 1 | 156 | 4.54e-39 | 136 |
| MS.gene88099.t1 | MTR_5g047580 | 44.805 | 154 | 83 | 2 | 4 | 157 | 3 | 154 | 4.80e-39 | 133 |
| MS.gene88099.t1 | MTR_2g016210 | 41.447 | 152 | 89 | 0 | 6 | 157 | 5 | 156 | 1.18e-38 | 135 |
| MS.gene88099.t1 | MTR_3g467080 | 40.260 | 154 | 92 | 0 | 4 | 157 | 3 | 156 | 3.03e-38 | 131 |
| MS.gene88099.t1 | MTR_1g077390 | 40.909 | 154 | 89 | 2 | 4 | 157 | 3 | 154 | 9.07e-35 | 122 |
| MS.gene88099.t1 | MTR_1g090783 | 39.610 | 154 | 91 | 2 | 4 | 157 | 2 | 153 | 1.54e-34 | 121 |
| MS.gene88099.t1 | MTR_1g090710 | 39.623 | 159 | 94 | 2 | 1 | 159 | 1 | 157 | 3.16e-34 | 120 |
| MS.gene88099.t1 | MTR_1g090697 | 39.744 | 156 | 92 | 2 | 4 | 159 | 3 | 156 | 8.25e-34 | 119 |
| MS.gene88099.t1 | MTR_3g465410 | 40.146 | 137 | 82 | 0 | 30 | 166 | 1 | 137 | 5.09e-33 | 117 |
| MS.gene88099.t1 | MTR_1g077320 | 42.857 | 154 | 86 | 2 | 4 | 157 | 3 | 154 | 3.11e-32 | 115 |
| MS.gene88099.t1 | MTR_7g011950 | 42.857 | 154 | 86 | 2 | 4 | 157 | 3 | 154 | 3.17e-32 | 115 |
| MS.gene88099.t1 | MTR_1g084950 | 43.506 | 154 | 85 | 2 | 4 | 157 | 3 | 154 | 4.11e-32 | 115 |
| MS.gene88099.t1 | MTR_4g028720 | 41.558 | 154 | 88 | 2 | 4 | 157 | 3 | 154 | 1.25e-30 | 111 |
| MS.gene88099.t1 | MTR_4g028800 | 78.788 | 66 | 12 | 1 | 6 | 71 | 5 | 68 | 1.29e-30 | 108 |
| MS.gene88099.t1 | MTR_1g077300 | 42.857 | 154 | 84 | 2 | 4 | 157 | 3 | 152 | 2.94e-29 | 108 |
| MS.gene88099.t1 | MTR_3g466900 | 38.462 | 130 | 79 | 1 | 33 | 162 | 2 | 130 | 4.39e-29 | 107 |
| MS.gene88099.t1 | MTR_3g031240 | 33.333 | 159 | 105 | 1 | 1 | 159 | 1 | 158 | 6.15e-29 | 108 |
| MS.gene88099.t1 | MTR_7g055800 | 50.427 | 117 | 30 | 2 | 4 | 120 | 3 | 91 | 1.90e-28 | 103 |
| MS.gene88099.t1 | MTR_3g466930 | 36.154 | 130 | 82 | 1 | 33 | 162 | 2 | 130 | 6.84e-27 | 101 |
| MS.gene88099.t1 | MTR_4g019670 | 39.103 | 156 | 79 | 2 | 3 | 158 | 5 | 144 | 1.05e-26 | 107 |
| MS.gene88099.t1 | MTR_7g106510 | 38.462 | 156 | 80 | 2 | 3 | 158 | 5 | 144 | 1.12e-26 | 107 |
| MS.gene88099.t1 | MTR_3g093900 | 29.016 | 193 | 126 | 2 | 3 | 195 | 5 | 186 | 4.44e-25 | 103 |
| MS.gene88099.t1 | MTR_7g055790 | 53.211 | 109 | 33 | 1 | 4 | 112 | 61 | 151 | 8.83e-25 | 96.3 |
| MS.gene88099.t1 | MTR_2g035590 | 59.794 | 97 | 38 | 1 | 75 | 170 | 1 | 97 | 9.54e-25 | 94.7 |
| MS.gene88099.t1 | MTR_2g035580 | 35.099 | 151 | 97 | 1 | 7 | 157 | 6 | 155 | 7.38e-24 | 94.0 |
| MS.gene88099.t1 | MTR_7g055940 | 49.038 | 104 | 25 | 2 | 4 | 107 | 3 | 78 | 9.21e-23 | 89.0 |
| MS.gene88099.t1 | MTR_5g047560 | 35.065 | 154 | 82 | 3 | 4 | 157 | 3 | 138 | 8.76e-21 | 85.5 |
| MS.gene88099.t1 | MTR_6g018920 | 32.432 | 111 | 68 | 4 | 4 | 108 | 3 | 112 | 5.52e-12 | 65.1 |
| MS.gene88099.t1 | MTR_6g005450 | 35.965 | 114 | 65 | 5 | 1 | 108 | 1 | 112 | 7.15e-12 | 64.7 |
| MS.gene88099.t1 | MTR_4g084780 | 57.692 | 52 | 22 | 0 | 75 | 126 | 1 | 52 | 1.29e-11 | 61.2 |
| MS.gene88099.t1 | MTR_1g114730 | 32.432 | 111 | 68 | 3 | 4 | 108 | 3 | 112 | 1.44e-11 | 63.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88099.t1 | AT5G48670 | 47.500 | 200 | 97 | 3 | 4 | 200 | 3 | 197 | 1.67e-46 | 157 |
| MS.gene88099.t1 | AT1G65300 | 40.761 | 184 | 95 | 3 | 4 | 187 | 2 | 171 | 1.47e-41 | 143 |
| MS.gene88099.t1 | AT1G65330 | 40.541 | 185 | 96 | 2 | 4 | 188 | 2 | 172 | 3.43e-39 | 137 |
| MS.gene88099.t1 | AT5G26630 | 41.379 | 203 | 102 | 3 | 4 | 204 | 3 | 190 | 9.64e-38 | 132 |
| MS.gene88099.t1 | AT5G26650 | 40.854 | 164 | 96 | 1 | 6 | 169 | 3 | 165 | 5.14e-37 | 134 |
| MS.gene88099.t1 | AT5G27960 | 40.244 | 164 | 97 | 1 | 6 | 169 | 3 | 165 | 2.46e-36 | 131 |
| MS.gene88099.t1 | AT1G31630 | 28.839 | 267 | 158 | 6 | 4 | 243 | 2 | 263 | 3.07e-35 | 128 |
| MS.gene88099.t1 | AT1G31640 | 39.073 | 151 | 90 | 2 | 4 | 154 | 2 | 150 | 5.34e-32 | 122 |
| MS.gene88099.t1 | AT5G27810 | 54.839 | 93 | 42 | 0 | 30 | 122 | 1 | 93 | 1.07e-30 | 110 |
| MS.gene88099.t1 | AT3G05860 | 39.189 | 148 | 89 | 1 | 4 | 151 | 3 | 149 | 4.68e-30 | 111 |
| MS.gene88099.t1 | AT3G05860 | 39.189 | 148 | 89 | 1 | 4 | 151 | 3 | 149 | 1.97e-29 | 111 |
| MS.gene88099.t1 | AT3G05860 | 39.189 | 148 | 89 | 1 | 4 | 151 | 3 | 149 | 1.97e-29 | 111 |
| MS.gene88099.t1 | AT2G28700 | 33.854 | 192 | 120 | 3 | 4 | 193 | 3 | 189 | 4.33e-28 | 109 |
| MS.gene88099.t1 | AT5G26580 | 36.538 | 156 | 83 | 3 | 1 | 156 | 1 | 140 | 4.30e-26 | 104 |
| MS.gene88099.t1 | AT1G22590 | 31.847 | 157 | 102 | 2 | 1 | 157 | 1 | 152 | 8.36e-25 | 96.7 |
| MS.gene88099.t1 | AT5G06500 | 34.868 | 152 | 97 | 2 | 4 | 155 | 3 | 152 | 1.90e-19 | 84.7 |
| MS.gene88099.t1 | AT2G40210 | 39.098 | 133 | 79 | 2 | 4 | 135 | 3 | 134 | 3.27e-17 | 80.1 |
| MS.gene88099.t1 | AT5G58890 | 38.583 | 127 | 67 | 5 | 7 | 124 | 6 | 130 | 5.20e-16 | 75.9 |
| MS.gene88099.t1 | AT5G55690 | 43.038 | 79 | 37 | 3 | 1 | 72 | 1 | 78 | 2.55e-12 | 65.5 |
| MS.gene88099.t1 | AT5G55690 | 43.038 | 79 | 37 | 3 | 1 | 72 | 1 | 78 | 2.55e-12 | 65.5 |
Find 0 sgRNAs with CRISPR-Local
Find 62 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGAAACAAAGAAAAGAAAAT+AGG | + | 33296:11379-11398 | MS.gene88099:CDS | 20.0% |
| !! | ATAATGTCATCTTCATTAAT+AGG | - | 33296:11687-11706 | MS.gene88099:intergenic | 20.0% |
| !! | GAAACAAAGAAAAGAAAATA+GGG | + | 33296:11380-11399 | MS.gene88099:CDS | 20.0% |
| ! | CAATGATCAAAATCAAATCA+TGG | + | 33296:11551-11570 | MS.gene88099:CDS | 25.0% |
| ! | TATGCAAAGTCAATTATTGA+TGG | + | 33296:11704-11723 | MS.gene88099:CDS | 25.0% |
| AGTGAAGAAATGGCAATTAT+GGG | + | 33296:11642-11661 | MS.gene88099:CDS | 30.0% | |
| ATCATACAAGAAAAGAACGA+AGG | + | 33296:11131-11150 | MS.gene88099:CDS | 30.0% | |
| ATGAGTCAATGTCTTAATGA+AGG | + | 33296:11423-11442 | MS.gene88099:CDS | 30.0% | |
| TCATACAAGAAAAGAACGAA+GGG | + | 33296:11132-11151 | MS.gene88099:CDS | 30.0% | |
| !! | TCAATTATTGATGGACTCAA+TGG | + | 33296:11713-11732 | MS.gene88099:CDS | 30.0% |
| AACGAAGGGTCTATTGAAAA+AGG | + | 33296:11146-11165 | MS.gene88099:CDS | 35.0% | |
| AAGAGACTTTCTTGACACAA+AGG | + | 33296:11331-11350 | MS.gene88099:CDS | 35.0% | |
| AGAGACTTTCTTGACACAAA+GGG | + | 33296:11332-11351 | MS.gene88099:CDS | 35.0% | |
| CATTAATAGGTATCCCAACA+TGG | - | 33296:11674-11693 | MS.gene88099:intergenic | 35.0% | |
| GAGTGAAGAAATGGCAATTA+TGG | + | 33296:11641-11660 | MS.gene88099:CDS | 35.0% | |
| GCATGACAATATGTCAACAA+TGG | + | 33296:11455-11474 | MS.gene88099:CDS | 35.0% | |
| GTTGATTGACCACAATTTGA+AGG | + | 33296:11497-11516 | MS.gene88099:CDS | 35.0% | |
| TACTAACGTCCTTCAAATTG+TGG | - | 33296:11509-11528 | MS.gene88099:intergenic | 35.0% | |
| TCATTGTCAATGATGTTGCA+AGG | + | 33296:11103-11122 | MS.gene88099:CDS | 35.0% | |
| TGCAACATCATTGACAATGA+AGG | - | 33296:11103-11122 | MS.gene88099:intergenic | 35.0% | |
| TGGATATGAGTTATCTTGCT+TGG | + | 33296:11475-11494 | MS.gene88099:CDS | 35.0% | |
| TTTGTGTCAAGAAAGTCTCT+TGG | - | 33296:11332-11351 | MS.gene88099:intergenic | 35.0% | |
| !! | ATTATTGATGGACTCAATGG+TGG | + | 33296:11716-11735 | MS.gene88099:CDS | 35.0% |
| !! | TGAATTTTGAGAGCACAGAT+TGG | - | 33296:11269-11288 | MS.gene88099:intergenic | 35.0% |
| AAACCAAGTCCAACTCGAAA+TGG | + | 33296:11581-11600 | MS.gene88099:CDS | 40.0% | |
| AGCATCTAGGAGTGAAGAAA+TGG | + | 33296:11632-11651 | MS.gene88099:CDS | 40.0% | |
| CTCATAAGGTCCATACACTA+TGG | - | 33296:11211-11230 | MS.gene88099:intergenic | 40.0% | |
| GAAATGGCAATTATGGGTCA+CGG | + | 33296:11648-11667 | MS.gene88099:CDS | 40.0% | |
| TCAATTTCTCTTCAGCCTTC+AGG | - | 33296:11359-11378 | MS.gene88099:intergenic | 40.0% | |
| TTAGTAGACGTTTGGAAGCA+TGG | + | 33296:11523-11542 | MS.gene88099:CDS | 40.0% | |
| ! | TAGTAGACGTTTGGAAGCAT+GGG | + | 33296:11524-11543 | MS.gene88099:CDS | 40.0% |
| ATGAGCCTCAACCTGAGATT+TGG | + | 33296:11226-11245 | MS.gene88099:CDS | 45.0% | |
| CCAGAATTGACCTCACCAAA+TGG | - | 33296:11765-11784 | MS.gene88099:intergenic | 45.0% | |
| GAAGGACGTTAGTAGACGTT+TGG | + | 33296:11515-11534 | MS.gene88099:CDS | 45.0% | |
| TCTGGTAAAGGCCAAATCTC+AGG | - | 33296:11240-11259 | MS.gene88099:intergenic | 45.0% | |
| ! | AAAGGCCAAATCTCAGGTTG+AGG | - | 33296:11234-11253 | MS.gene88099:intergenic | 45.0% |
| ! | AGATTTGGCCTTTACCAGAG+GGG | + | 33296:11241-11260 | MS.gene88099:CDS | 45.0% |
| ! | CCATTTGGTGAGGTCAATTC+TGG | + | 33296:11762-11781 | MS.gene88099:CDS | 45.0% |
| ! | GAGATTTGGCCTTTACCAGA+GGG | + | 33296:11240-11259 | MS.gene88099:CDS | 45.0% |
| ! | TGAGATTTGGCCTTTACCAG+AGG | + | 33296:11239-11258 | MS.gene88099:CDS | 45.0% |
| !! | ATCTCAGGTTGAGGCTCATA+AGG | - | 33296:11225-11244 | MS.gene88099:intergenic | 45.0% |
| !! | GTGAGGTCAATTCTGGAGTT+TGG | + | 33296:11769-11788 | MS.gene88099:CDS | 45.0% |
| ACAGGCTTCAATGCCACAGA+GGG | - | 33296:11187-11206 | MS.gene88099:intergenic | 50.0% | |
| AGGTCCATACACTATGGCAC+AGG | - | 33296:11205-11224 | MS.gene88099:intergenic | 50.0% | |
| ATTATGGGTCACGGCCATGT+TGG | + | 33296:11657-11676 | MS.gene88099:CDS | 50.0% | |
| CACCACTTCTAGCAGCATCT+AGG | + | 33296:11619-11638 | MS.gene88099:CDS | 50.0% | |
| CCAAGTCCAACTCGAAATGG+CGG | + | 33296:11584-11603 | MS.gene88099:CDS | 50.0% | |
| CTCCTAGATGCTGCTAGAAG+TGG | - | 33296:11624-11643 | MS.gene88099:intergenic | 50.0% | |
| CTTGACACAAAGGGTCCTGA+AGG | + | 33296:11341-11360 | MS.gene88099:CDS | 50.0% | |
| GAAGCCTGTGCCATAGTGTA+TGG | + | 33296:11198-11217 | MS.gene88099:CDS | 50.0% | |
| GATGCTGCTAGAAGTGGTGA+TGG | - | 33296:11618-11637 | MS.gene88099:intergenic | 50.0% | |
| TTATGGGTCACGGCCATGTT+GGG | + | 33296:11658-11677 | MS.gene88099:CDS | 50.0% | |
| ! | GATTTGGCCTTTACCAGAGG+GGG | + | 33296:11242-11261 | MS.gene88099:CDS | 50.0% |
| ! | GTGCAAGAGACAGTGCCATT+TGG | + | 33296:11747-11766 | MS.gene88099:CDS | 50.0% |
| ! | TTGTTGCCGCCATTTCGAGT+TGG | - | 33296:11593-11612 | MS.gene88099:intergenic | 50.0% |
| !! | AGAGACAGTGCCATTTGGTG+AGG | + | 33296:11752-11771 | MS.gene88099:CDS | 50.0% |
| !! | TTGATGGACTCAATGGTGGC+TGG | + | 33296:11720-11739 | MS.gene88099:CDS | 50.0% |
| CACAGGCTTCAATGCCACAG+AGG | - | 33296:11188-11207 | MS.gene88099:intergenic | 55.0% | |
| GATTGGACCCCCTCTGGTAA+AGG | - | 33296:11252-11271 | MS.gene88099:intergenic | 55.0% | |
| GCTGAACTTAGCACCCTCTG+TGG | + | 33296:11171-11190 | MS.gene88099:CDS | 55.0% | |
| !! | CCGCCATTTCGAGTTGGACT+TGG | - | 33296:11587-11606 | MS.gene88099:intergenic | 55.0% |
| AGCACAGATTGGACCCCCTC+TGG | - | 33296:11258-11277 | MS.gene88099:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 33296 | gene | 11072 | 11809 | 11072 | ID=MS.gene88099 |
| 33296 | mRNA | 11072 | 11809 | 11072 | ID=MS.gene88099.t1;Parent=MS.gene88099 |
| 33296 | exon | 11072 | 11809 | 11072 | ID=MS.gene88099.t1.exon1;Parent=MS.gene88099.t1 |
| 33296 | CDS | 11072 | 11809 | 11072 | ID=cds.MS.gene88099.t1;Parent=MS.gene88099.t1 |
| Gene Sequence |
| Protein sequence |
