Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88106.t1 | RHN76722.1 | 87.5 | 56 | 7 | 0 | 49 | 104 | 504 | 559 | 9.10E-17 | 96.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88106.t1 | Q9FJK3 | 53.9 | 76 | 31 | 1 | 4 | 79 | 3 | 74 | 7.5e-12 | 71.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88106.t1 | G7I3H5 | 87.5 | 56 | 7 | 0 | 49 | 104 | 920 | 975 | 6.6e-17 | 96.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene88106.t1 | TF | MADS-M-type |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88106.t1 | MTR_1g007990 | 75.926 | 54 | 13 | 0 | 41 | 94 | 912 | 965 | 3.57e-22 | 89.7 |
| MS.gene88106.t1 | MTR_8g036130 | 80.000 | 45 | 9 | 0 | 4 | 48 | 3 | 47 | 1.85e-17 | 74.7 |
| MS.gene88106.t1 | MTR_4g032620 | 75.000 | 48 | 11 | 1 | 1 | 48 | 1 | 47 | 3.39e-17 | 73.9 |
| MS.gene88106.t1 | MTR_1g090697 | 41.593 | 113 | 45 | 5 | 4 | 99 | 3 | 111 | 5.59e-16 | 68.9 |
| MS.gene88106.t1 | MTR_1g090783 | 54.839 | 62 | 24 | 1 | 4 | 65 | 2 | 59 | 5.71e-16 | 68.9 |
| MS.gene88106.t1 | MTR_5g047580 | 40.179 | 112 | 48 | 2 | 4 | 100 | 3 | 110 | 6.09e-16 | 68.9 |
| MS.gene88106.t1 | MTR_1g077390 | 36.937 | 111 | 51 | 3 | 4 | 99 | 3 | 109 | 1.26e-14 | 65.5 |
| MS.gene88106.t1 | MTR_3g466830 | 68.889 | 45 | 14 | 0 | 4 | 48 | 2 | 46 | 1.82e-14 | 65.5 |
| MS.gene88106.t1 | MTR_4g028800 | 74.419 | 43 | 9 | 1 | 6 | 48 | 5 | 45 | 2.09e-14 | 62.8 |
| MS.gene88106.t1 | MTR_2g016210 | 72.093 | 43 | 12 | 0 | 6 | 48 | 5 | 47 | 2.61e-14 | 66.2 |
| MS.gene88106.t1 | MTR_3g466890 | 44.444 | 90 | 45 | 2 | 4 | 93 | 2 | 86 | 5.76e-14 | 63.9 |
| MS.gene88106.t1 | MTR_3g466980 | 64.444 | 45 | 16 | 0 | 4 | 48 | 3 | 47 | 1.37e-13 | 63.9 |
| MS.gene88106.t1 | MTR_4g031910 | 48.598 | 107 | 44 | 3 | 4 | 104 | 3 | 104 | 2.62e-13 | 63.5 |
| MS.gene88106.t1 | MTR_3g031240 | 33.945 | 109 | 60 | 4 | 1 | 103 | 1 | 103 | 3.78e-13 | 62.4 |
| MS.gene88106.t1 | MTR_3g065100 | 44.037 | 109 | 49 | 3 | 4 | 100 | 3 | 111 | 1.51e-12 | 61.6 |
| MS.gene88106.t1 | MTR_4g063790 | 54.348 | 46 | 21 | 0 | 4 | 49 | 3 | 48 | 3.60e-12 | 59.7 |
| MS.gene88106.t1 | MTR_5g075380 | 62.791 | 43 | 16 | 0 | 6 | 48 | 5 | 47 | 3.83e-12 | 59.3 |
| MS.gene88106.t1 | MTR_2g035610 | 80.000 | 45 | 9 | 0 | 4 | 48 | 3 | 47 | 4.17e-12 | 58.2 |
| MS.gene88106.t1 | MTR_1g090710 | 48.438 | 64 | 28 | 2 | 1 | 64 | 1 | 59 | 4.76e-12 | 58.9 |
| MS.gene88106.t1 | MTR_4g028720 | 47.191 | 89 | 41 | 2 | 4 | 92 | 3 | 85 | 5.15e-12 | 58.9 |
| MS.gene88106.t1 | MTR_4g019670 | 36.036 | 111 | 59 | 3 | 3 | 105 | 5 | 111 | 1.62e-11 | 59.3 |
| MS.gene88106.t1 | MTR_7g106510 | 34.234 | 111 | 61 | 3 | 3 | 105 | 5 | 111 | 2.68e-11 | 58.9 |
| MS.gene88106.t1 | MTR_7g011950 | 47.191 | 89 | 41 | 2 | 4 | 92 | 3 | 85 | 6.07e-11 | 56.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88106.t1 | AT3G05860 | 43.182 | 88 | 45 | 2 | 4 | 91 | 3 | 85 | 1.76e-15 | 68.9 |
| MS.gene88106.t1 | AT3G05860 | 43.182 | 88 | 45 | 2 | 4 | 91 | 3 | 85 | 2.74e-15 | 68.9 |
| MS.gene88106.t1 | AT3G05860 | 43.182 | 88 | 45 | 2 | 4 | 91 | 3 | 85 | 3.36e-15 | 68.9 |
| MS.gene88106.t1 | AT1G22590 | 42.308 | 78 | 37 | 3 | 1 | 75 | 1 | 73 | 3.06e-12 | 59.7 |
| MS.gene88106.t1 | AT5G26580 | 35.000 | 100 | 60 | 3 | 1 | 99 | 1 | 96 | 5.06e-12 | 60.8 |
| MS.gene88106.t1 | AT1G31630 | 34.653 | 101 | 54 | 4 | 4 | 96 | 2 | 98 | 2.94e-11 | 58.5 |
| MS.gene88106.t1 | AT1G65330 | 55.556 | 45 | 20 | 0 | 4 | 48 | 2 | 46 | 5.65e-11 | 57.8 |
| MS.gene88106.t1 | AT5G26650 | 31.776 | 107 | 59 | 3 | 6 | 99 | 3 | 108 | 6.75e-11 | 57.8 |
Find 0 sgRNAs with CRISPR-Local
Find 24 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTCATATTTGTATTATGTAT+AGG | + | 33309:599-618 | MS.gene88106:intron | 15.0% |
| !! | TAAAAAACAGAATAGTTAGA+TGG | - | 33309:642-661 | MS.gene88106:intergenic | 20.0% |
| !!! | ATCTAACTATTCTGTTTTTT+AGG | + | 33309:641-660 | MS.gene88106:intron | 20.0% |
| ! | TTACTACTGAAGATTGAAAA+AGG | + | 33309:754-773 | MS.gene88106:CDS | 25.0% |
| !! | GTATTATGTATAGGTTTTTG+AGG | + | 33309:608-627 | MS.gene88106:intron | 25.0% |
| GAAGAAGAGTCTATTCAAAA+AGG | + | 33309:484-503 | MS.gene88106:CDS | 30.0% | |
| ! | CTTGCATATATTTTCCTTGT+GGG | + | 33309:668-687 | MS.gene88106:CDS | 30.0% |
| CCACAAGGAAAATATATGCA+AGG | - | 33309:670-689 | MS.gene88105:intergenic | 35.0% | |
| GGAAAGCAACATACAAGAAA+AGG | + | 33309:462-481 | MS.gene88106:CDS | 35.0% | |
| TCATTGTCAATGATGCTTCT+AGG | + | 33309:441-460 | MS.gene88106:CDS | 35.0% | |
| ! | CCTTGCATATATTTTCCTTG+TGG | + | 33309:667-686 | MS.gene88106:CDS | 35.0% |
| !! | AGAAGCATCATTGACAATGA+AGG | - | 33309:441-460 | MS.gene88106:intergenic | 35.0% |
| ACAAGCCTCAATACCACATA+GGG | - | 33309:525-544 | MS.gene88105:intergenic | 40.0% | |
| ATATCCATCTTGTTCCCACA+AGG | - | 33309:685-704 | MS.gene88105:intergenic | 40.0% | |
| GAAATGCAGAACTCTCGTAA+AGG | - | 33309:562-581 | MS.gene88105:intergenic | 40.0% | |
| TTCAACATTTCCGTAGCTTC+TGG | - | 33309:712-731 | MS.gene88106:intergenic | 40.0% | |
| ! | TTTTCCTTGTGGGAACAAGA+TGG | + | 33309:678-697 | MS.gene88106:CDS | 40.0% |
| AACATTTCCGTAGCTTCTGG+AGG | - | 33309:709-728 | MS.gene88105:intergenic | 45.0% | |
| CACAAGCCTCAATACCACAT+AGG | - | 33309:526-545 | MS.gene88105:intergenic | 45.0% | |
| CTCGTAAAGGCCATACACTA+TGG | - | 33309:549-568 | MS.gene88105:intergenic | 45.0% | |
| GGATATTCCTCCAGAAGCTA+CGG | + | 33309:699-718 | MS.gene88106:CDS | 45.0% | |
| GTTGAACTTAGCACCCTATG+TGG | + | 33309:509-528 | MS.gene88106:CDS | 45.0% | |
| TAGCACCCTATGTGGTATTG+AGG | + | 33309:517-536 | MS.gene88106:CDS | 45.0% | |
| GAGGCTTGTGCCATAGTGTA+TGG | + | 33309:536-555 | MS.gene88106:intron | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 33309 | gene | 410 | 837 | 410 | ID=MS.gene88106 |
| 33309 | mRNA | 410 | 837 | 410 | ID=MS.gene88106.t1;Parent=MS.gene88106 |
| 33309 | exon | 410 | 552 | 410 | ID=MS.gene88106.t1.exon1;Parent=MS.gene88106.t1 |
| 33309 | CDS | 410 | 552 | 410 | ID=cds.MS.gene88106.t1;Parent=MS.gene88106.t1 |
| 33309 | exon | 663 | 837 | 663 | ID=MS.gene88106.t1.exon2;Parent=MS.gene88106.t1 |
| 33309 | CDS | 663 | 837 | 663 | ID=cds.MS.gene88106.t1;Parent=MS.gene88106.t1 |
| Gene Sequence |
| Protein sequence |
