AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene88151

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene88151.t1 MTR_4g031910 72.650 234 47 2 1 222 1 229 3.29e-114 326
MS.gene88151.t1 MTR_4g032290 69.672 244 39 2 1 222 1 231 7.05e-111 317
MS.gene88151.t1 MTR_4g032620 57.576 231 89 1 1 222 1 231 4.39e-93 272
MS.gene88151.t1 MTR_8g036130 56.680 247 82 4 1 222 1 247 3.95e-89 263
MS.gene88151.t1 MTR_4g032260 80.892 157 30 0 1 157 1 157 2.30e-82 243
MS.gene88151.t1 MTR_3g065100 49.793 241 96 4 1 219 1 238 1.80e-66 205
MS.gene88151.t1 MTR_3g466980 45.690 232 101 5 1 218 1 221 4.59e-64 198
MS.gene88151.t1 MTR_2g035610 72.131 122 34 0 1 122 1 122 1.71e-50 160
MS.gene88151.t1 MTR_3g466830 49.669 151 75 1 3 153 2 151 4.33e-47 153
MS.gene88151.t1 MTR_4g032270 56.204 137 45 2 94 222 2 131 7.63e-46 149
MS.gene88151.t1 MTR_5g075380 47.097 155 81 1 1 155 1 154 4.97e-45 148
MS.gene88151.t1 MTR_4g063790 44.805 154 85 0 1 154 1 154 1.24e-44 147
MS.gene88151.t1 MTR_3g031100 35.268 224 133 4 1 219 1 217 1.97e-42 144
MS.gene88151.t1 MTR_3g467080 42.949 156 89 0 1 156 1 156 2.07e-42 141
MS.gene88151.t1 MTR_3g466890 46.053 152 81 1 3 154 2 152 3.33e-41 138
MS.gene88151.t1 MTR_5g047580 46.154 156 82 2 1 156 1 154 6.82e-41 137
MS.gene88151.t1 MTR_2g016210 39.560 182 108 2 1 180 1 182 3.65e-40 138
MS.gene88151.t1 MTR_1g077390 42.308 156 88 2 1 156 1 154 5.29e-37 127
MS.gene88151.t1 MTR_1g090783 42.208 154 87 2 3 156 2 153 3.33e-35 122
MS.gene88151.t1 MTR_1g077320 44.231 156 85 2 1 156 1 154 2.39e-34 120
MS.gene88151.t1 MTR_3g465410 43.651 126 71 0 29 154 1 126 5.46e-34 119
MS.gene88151.t1 MTR_7g011950 43.590 156 86 2 1 156 1 154 7.76e-34 119
MS.gene88151.t1 MTR_4g028720 44.231 156 85 2 1 156 1 154 1.09e-33 119
MS.gene88151.t1 MTR_1g084950 44.872 156 84 2 1 156 1 154 2.14e-33 118
MS.gene88151.t1 MTR_1g090710 38.961 154 93 1 1 154 1 153 6.53e-33 116
MS.gene88151.t1 MTR_1g090697 40.000 150 88 2 1 150 1 148 5.96e-32 114
MS.gene88151.t1 MTR_1g077300 43.590 156 84 2 1 156 1 152 7.52e-32 114
MS.gene88151.t1 MTR_4g028800 77.143 70 14 1 1 70 1 68 2.98e-31 109
MS.gene88151.t1 MTR_3g031240 34.615 156 102 0 1 156 1 156 1.23e-30 111
MS.gene88151.t1 MTR_3g466900 41.463 123 71 1 32 154 2 123 5.13e-29 105
MS.gene88151.t1 MTR_7g055800 49.573 117 31 2 1 117 1 89 3.65e-28 102
MS.gene88151.t1 MTR_2g035580 37.580 157 97 1 1 157 1 156 3.27e-26 99.8
MS.gene88151.t1 MTR_3g466930 39.024 123 74 1 32 154 2 123 5.98e-26 98.2
MS.gene88151.t1 MTR_7g055790 51.351 111 36 1 1 111 59 151 3.35e-25 96.7
MS.gene88151.t1 MTR_7g106510 39.370 127 77 0 3 129 6 132 2.46e-24 100
MS.gene88151.t1 MTR_4g019670 40.157 127 76 0 3 129 6 132 4.93e-24 99.8
MS.gene88151.t1 MTR_5g047560 36.538 156 81 3 1 156 1 138 4.32e-23 90.9
MS.gene88151.t1 MTR_7g055940 48.113 106 27 2 1 106 1 78 6.75e-23 88.6
MS.gene88151.t1 MTR_3g093900 31.690 142 97 0 3 144 6 147 1.12e-21 93.2
MS.gene88151.t1 MTR_2g035590 56.790 81 35 0 74 154 1 81 1.82e-19 80.1
MS.gene88151.t1 MTR_1g114730 31.858 113 70 3 1 107 1 112 2.31e-13 68.6
MS.gene88151.t1 MTR_6g018920 32.743 113 69 4 1 107 1 112 5.53e-13 67.4
MS.gene88151.t1 MTR_6g005450 33.628 113 68 4 1 107 1 112 3.74e-12 65.1
MS.gene88151.t1 MTR_4g084780 38.739 111 52 2 74 168 1 111 9.03e-12 61.2
MS.gene88151.t1 MTR_6g005440 35.398 113 66 3 1 107 1 112 2.08e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene88151.t1 AT5G48670 55.844 154 67 1 1 154 1 153 7.79e-46 155
MS.gene88151.t1 AT1G65330 42.246 187 94 3 3 176 2 187 1.51e-40 140
MS.gene88151.t1 AT1G65300 41.436 181 97 2 3 175 2 181 2.08e-40 139
MS.gene88151.t1 AT5G26630 43.979 191 96 2 1 181 1 190 1.02e-38 133
MS.gene88151.t1 AT5G26650 36.190 210 115 4 5 204 3 203 1.11e-34 127
MS.gene88151.t1 AT5G27960 39.881 168 90 3 5 162 3 169 3.98e-34 124
MS.gene88151.t1 AT1G31630 36.905 168 95 3 3 161 2 167 1.35e-33 123
MS.gene88151.t1 AT1G31640 39.735 151 89 2 3 153 2 150 6.93e-32 121
MS.gene88151.t1 AT3G05860 37.975 158 97 1 1 158 1 157 1.18e-30 112
MS.gene88151.t1 AT3G05860 37.975 158 97 1 1 158 1 157 1.88e-30 113
MS.gene88151.t1 AT3G05860 37.975 158 97 1 1 158 1 157 2.12e-30 113
MS.gene88151.t1 AT5G27810 55.914 93 41 0 29 121 1 93 7.87e-30 107
MS.gene88151.t1 AT2G28700 36.810 163 98 2 1 161 1 160 1.44e-28 110
MS.gene88151.t1 AT1G22590 32.099 162 106 1 1 162 1 158 5.74e-26 99.4
MS.gene88151.t1 AT5G26580 34.416 154 86 2 1 154 1 139 2.55e-23 96.3
MS.gene88151.t1 AT5G06500 35.669 157 99 2 1 157 1 155 6.68e-23 93.2
MS.gene88151.t1 AT2G40210 37.209 129 80 1 1 129 1 128 1.35e-17 80.5
MS.gene88151.t1 AT5G58890 37.795 127 68 5 6 123 6 130 2.21e-15 73.6
MS.gene88151.t1 AT5G55690 42.308 78 38 2 1 71 1 78 2.29e-12 65.1
MS.gene88151.t1 AT5G55690 42.308 78 38 2 1 71 1 78 2.29e-12 65.1
MS.gene88151.t1 AT2G15660 30.088 113 74 2 38 150 31 138 3.26e-11 61.6
MS.gene88151.t1 AT5G27090 34.444 90 58 1 7 95 23 112 7.04e-11 59.7
MS.gene88151.t1 AT5G27944 38.095 84 48 2 24 103 41 124 8.20e-11 60.1

Find 58 sgRNAs with CRISPR-Local

Find 59 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCCTTTGGTGAGATCAATTA+TGG 0.234667 4.3:-68920690 MS.gene88151:CDS
TACCAATGTCCTTCAAATTA+TGG 0.254451 4.3:+68920880 None:intergenic
TGAAACAAAGAAAAGAAAAT+AGG 0.262024 4.3:-68921007 MS.gene88151:CDS
ATAATTTCATCATCATTAAT+AGG 0.285530 4.3:+68920768 None:intergenic
GTGAGATCAATTATGGAGTT+TGG 0.309548 4.3:-68920683 MS.gene88151:CDS
AACAAAGGGTCTATTGAAAA+AGG 0.314581 4.3:-68921240 MS.gene88151:CDS
AGCATCTAGGAGTGAGGAAA+TGG 0.344298 4.3:-68920820 MS.gene88151:CDS
ATGAGCCTCAACCTGAGATT+TGG 0.345571 4.3:-68921160 MS.gene88151:CDS
ATCCTTCAAGGTAGTAAATC+AGG 0.372648 4.3:+68920660 None:intergenic
TATGCAAAGTCAATTATTGA+TGG 0.385590 4.3:-68920748 MS.gene88151:CDS
GAAGGACATTGGTAGACGTT+TGG 0.410243 4.3:-68920871 MS.gene88151:CDS
CTAGATGCTGTTGGAGGTGG+TGG 0.417295 4.3:+68920834 None:intergenic
GAAACAAAGAAAAGAAAATA+GGG 0.419680 4.3:-68921006 MS.gene88151:CDS
GGCCTGATTTACTACCTTGA+AGG 0.422090 4.3:-68920662 None:intergenic
AGTGAGGAAATGGGAATTGT+GGG 0.443118 4.3:-68920810 MS.gene88151:CDS
GTTGATTGACCATAATTTGA+AGG 0.445081 4.3:-68920889 MS.gene88151:CDS
TTGTGGGTCATGGCCATGTT+GGG 0.452922 4.3:-68920794 MS.gene88151:CDS
CACAACCATGGCTAGAAGTA+AGG 0.457305 4.3:-68921318 None:intergenic
TCTGGTGAAGGCCAAATCTC+AGG 0.467267 4.3:+68921149 None:intergenic
CTCATAAGAGCCATACACTA+TGG 0.467809 4.3:+68921178 None:intergenic
ATTGTGGGTCATGGCCATGT+TGG 0.468248 4.3:-68920795 MS.gene88151:CDS
CTCCTAGATGCTGTTGGAGG+TGG 0.476689 4.3:+68920831 None:intergenic
TCAATTATTGATGGACTCAA+TGG 0.482426 4.3:-68920739 MS.gene88151:CDS
TTGATTTGAGTTATCTTACT+TGG 0.482489 4.3:-68920911 MS.gene88151:CDS
TCCTCACTCCTAGATGCTGT+TGG 0.486555 4.3:+68920825 None:intergenic
CTTGACACAAAGGATTCTGA+AGG 0.491635 4.3:-68921045 MS.gene88151:CDS
TGCAGCATCATTGACAATGA+AGG 0.502295 4.3:+68921286 None:intergenic
TTTGTGTCAAGAAAGTCTCT+TGG 0.512375 4.3:+68921057 None:intergenic
GATGCTGTTGGAGGTGGTGG+TGG 0.513228 4.3:+68920837 None:intergenic
GAGTGAGGAAATGGGAATTG+TGG 0.516317 4.3:-68920811 MS.gene88151:CDS
GACCATAATTTGAAGGACAT+TGG 0.517963 4.3:-68920882 MS.gene88151:CDS
GGACATTGGTAGACGTTTGG+CGG 0.524285 4.3:-68920868 MS.gene88151:CDS
TCACTCCTAGATGCTGTTGG+AGG 0.527908 4.3:+68920828 None:intergenic
CACCACCTCCAACAGCATCT+AGG 0.540352 4.3:-68920833 MS.gene88151:CDS
GCATCTAGGAGTGAGGAAAT+GGG 0.544227 4.3:-68920819 MS.gene88151:CDS
ATGTCTCATTGTCTTAATGA+AGG 0.544347 4.3:-68920963 MS.gene88151:CDS
CCATAATTGATCTCACCAAA+GGG 0.558369 4.3:+68920690 None:intergenic
GAAGCCTGTGCCATAGTGTA+TGG 0.560968 4.3:-68921188 MS.gene88151:CDS
GAGATTTGGCCTTCACCAGA+GGG 0.565461 4.3:-68921146 MS.gene88151:CDS
TTTAACCTTACTTCTAGCCA+TGG 0.572129 4.3:+68921313 None:intergenic
GATTTGGCCTTCACCAGAGG+GGG 0.573799 4.3:-68921144 MS.gene88151:CDS
TCCATAATTGATCTCACCAA+AGG 0.580970 4.3:+68920689 None:intergenic
GTGCACGAGACAGTGCCCTT+TGG 0.582311 4.3:-68920705 MS.gene88151:CDS
AAGAGACTTTCTTGACACAA+AGG 0.587821 4.3:-68921055 MS.gene88151:CDS
CATTAATAGGTATCCCAACA+TGG 0.589301 4.3:+68920781 None:intergenic
GAAGGCCAAATCTCAGGTTG+AGG 0.598100 4.3:+68921155 None:intergenic
ACAGGCTTCAATGCCACAGA+GGG 0.601811 4.3:+68921202 None:intergenic
TTGATGGACTCAATGGTGGC+TGG 0.607204 4.3:-68920732 MS.gene88151:CDS
TGAGATTTGGCCTTCACCAG+AGG 0.614269 4.3:-68921147 MS.gene88151:CDS
GAAATGGGAATTGTGGGTCA+TGG 0.616732 4.3:-68920804 MS.gene88151:CDS
TCCAACAGCATCTAGGAGTG+AGG 0.643310 4.3:-68920826 MS.gene88151:CDS
AGATTTGGCCTTCACCAGAG+GGG 0.650878 4.3:-68921145 MS.gene88151:CDS
TCATTGTCAATGATGCTGCA+AGG 0.652548 4.3:-68921283 MS.gene88151:CDS
AGAGCCATACACTATGGCAC+AGG 0.656790 4.3:+68921184 None:intergenic
ATTATTGATGGACTCAATGG+TGG 0.666274 4.3:-68920736 MS.gene88151:CDS
GCTGAACTTAGCACCCTCTG+TGG 0.677042 4.3:-68921215 MS.gene88151:CDS
TGTGCACGACAATATGTCAA+CGG 0.688304 4.3:-68920934 MS.gene88151:CDS
CACAGGCTTCAATGCCACAG+AGG 0.696692 4.3:+68921201 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAATTTCATCATCATTAAT+AGG + chr4.3:68921211-68921230 None:intergenic 15.0%
!! AACATACAAAAAAAGAACAA+AGG - chr4.3:68920721-68920740 MS.gene88151:CDS 20.0%
!! ACATACAAAAAAAGAACAAA+GGG - chr4.3:68920722-68920741 MS.gene88151:CDS 20.0%
!! GAAACAAAGAAAAGAAAATA+GGG - chr4.3:68920970-68920989 MS.gene88151:CDS 20.0%
!! TGAAACAAAGAAAAGAAAAT+AGG - chr4.3:68920969-68920988 MS.gene88151:CDS 20.0%
! TATGCAAAGTCAATTATTGA+TGG - chr4.3:68921228-68921247 MS.gene88151:CDS 25.0%
!! TTGATTTGAGTTATCTTACT+TGG - chr4.3:68921065-68921084 MS.gene88151:CDS 25.0%
AACAAAGGGTCTATTGAAAA+AGG - chr4.3:68920736-68920755 MS.gene88151:CDS 30.0%
ATGTCTCATTGTCTTAATGA+AGG - chr4.3:68921013-68921032 MS.gene88151:CDS 30.0%
GTTGATTGACCATAATTTGA+AGG - chr4.3:68921087-68921106 MS.gene88151:CDS 30.0%
TACCAATGTCCTTCAAATTA+TGG + chr4.3:68921099-68921118 None:intergenic 30.0%
!! TCAATTATTGATGGACTCAA+TGG - chr4.3:68921237-68921256 MS.gene88151:CDS 30.0%
AAGAGACTTTCTTGACACAA+AGG - chr4.3:68920921-68920940 MS.gene88151:CDS 35.0%
CATTAATAGGTATCCCAACA+TGG + chr4.3:68921198-68921217 None:intergenic 35.0%
CCATAATTGATCTCACCAAA+GGG + chr4.3:68921289-68921308 None:intergenic 35.0%
GACCATAATTTGAAGGACAT+TGG - chr4.3:68921094-68921113 MS.gene88151:CDS 35.0%
GTGAGATCAATTATGGAGTT+TGG - chr4.3:68921293-68921312 MS.gene88151:CDS 35.0%
TCCATAATTGATCTCACCAA+AGG + chr4.3:68921290-68921309 None:intergenic 35.0%
TTTGTGTCAAGAAAGTCTCT+TGG + chr4.3:68920922-68920941 None:intergenic 35.0%
!! ATTATTGATGGACTCAATGG+TGG - chr4.3:68921240-68921259 MS.gene88151:CDS 35.0%
!! TGAATTTTGAGAGCACAGAT+TGG + chr4.3:68920859-68920878 None:intergenic 35.0%
AGTGAGGAAATGGGAATTGT+GGG - chr4.3:68921166-68921185 MS.gene88151:CDS 40.0%
CCCTTTGGTGAGATCAATTA+TGG - chr4.3:68921286-68921305 MS.gene88151:CDS 40.0%
CTCATAAGAGCCATACACTA+TGG + chr4.3:68920801-68920820 None:intergenic 40.0%
CTTGACACAAAGGATTCTGA+AGG - chr4.3:68920931-68920950 MS.gene88151:CDS 40.0%
TCATTGTCAATGATGCTGCA+AGG - chr4.3:68920693-68920712 MS.gene88151:CDS 40.0%
TGCAGCATCATTGACAATGA+AGG + chr4.3:68920693-68920712 None:intergenic 40.0%
TGTGCACGACAATATGTCAA+CGG - chr4.3:68921042-68921061 MS.gene88151:CDS 40.0%
AGCATCTAGGAGTGAGGAAA+TGG - chr4.3:68921156-68921175 MS.gene88151:CDS 45.0%
ATGAGCCTCAACCTGAGATT+TGG - chr4.3:68920816-68920835 MS.gene88151:CDS 45.0%
GAAATGGGAATTGTGGGTCA+TGG - chr4.3:68921172-68921191 MS.gene88151:CDS 45.0%
GAAGGACATTGGTAGACGTT+TGG - chr4.3:68921105-68921124 MS.gene88151:CDS 45.0%
GAGTGAGGAAATGGGAATTG+TGG - chr4.3:68921165-68921184 MS.gene88151:CDS 45.0%
GCATCTAGGAGTGAGGAAAT+GGG - chr4.3:68921157-68921176 MS.gene88151:CDS 45.0%
ACAGGCTTCAATGCCACAGA+GGG + chr4.3:68920777-68920796 None:intergenic 50.0%
AGAGCCATACACTATGGCAC+AGG + chr4.3:68920795-68920814 None:intergenic 50.0%
ATTGTGGGTCATGGCCATGT+TGG - chr4.3:68921181-68921200 MS.gene88151:CDS 50.0%
GAAGCCTGTGCCATAGTGTA+TGG - chr4.3:68920788-68920807 MS.gene88151:CDS 50.0%
GGACATTGGTAGACGTTTGG+CGG - chr4.3:68921108-68921127 MS.gene88151:CDS 50.0%
TCACTCCTAGATGCTGTTGG+AGG + chr4.3:68921151-68921170 None:intergenic 50.0%
TCCAACAGCATCTAGGAGTG+AGG - chr4.3:68921150-68921169 MS.gene88151:CDS 50.0%
TCCTCACTCCTAGATGCTGT+TGG + chr4.3:68921154-68921173 None:intergenic 50.0%
TCTGGTGAAGGCCAAATCTC+AGG + chr4.3:68920830-68920849 None:intergenic 50.0%
TTGTGGGTCATGGCCATGTT+GGG - chr4.3:68921182-68921201 MS.gene88151:CDS 50.0%
! AGATTTGGCCTTCACCAGAG+GGG - chr4.3:68920831-68920850 MS.gene88151:CDS 50.0%
! GAAGGCCAAATCTCAGGTTG+AGG + chr4.3:68920824-68920843 None:intergenic 50.0%
! GAGATTTGGCCTTCACCAGA+GGG - chr4.3:68920830-68920849 MS.gene88151:CDS 50.0%
! TGAGATTTGGCCTTCACCAG+AGG - chr4.3:68920829-68920848 MS.gene88151:CDS 50.0%
!! TTGATGGACTCAATGGTGGC+TGG - chr4.3:68921244-68921263 MS.gene88151:CDS 50.0%
CACAGGCTTCAATGCCACAG+AGG + chr4.3:68920778-68920797 None:intergenic 55.0%
CACCACCTCCAACAGCATCT+AGG - chr4.3:68921143-68921162 MS.gene88151:CDS 55.0%
CTAGATGCTGTTGGAGGTGG+TGG + chr4.3:68921145-68921164 None:intergenic 55.0%
CTCCTAGATGCTGTTGGAGG+TGG + chr4.3:68921148-68921167 None:intergenic 55.0%
GCTGAACTTAGCACCCTCTG+TGG - chr4.3:68920761-68920780 MS.gene88151:CDS 55.0%
! GATTTGGCCTTCACCAGAGG+GGG - chr4.3:68920832-68920851 MS.gene88151:CDS 55.0%
AGCACAGATTGGACCCCCTC+TGG + chr4.3:68920848-68920867 None:intergenic 60.0%
GATTGGACCCCCTCTGGTGA+AGG + chr4.3:68920842-68920861 None:intergenic 60.0%
GTGCACGAGACAGTGCCCTT+TGG - chr4.3:68921271-68921290 MS.gene88151:CDS 60.0%
! GATGCTGTTGGAGGTGGTGG+TGG + chr4.3:68921142-68921161 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 68920665 68921333 68920665 ID=MS.gene88151
chr4.3 mRNA 68920665 68921333 68920665 ID=MS.gene88151.t1;Parent=MS.gene88151
chr4.3 exon 68920665 68921333 68920665 ID=MS.gene88151.t1.exon1;Parent=MS.gene88151.t1
chr4.3 CDS 68920665 68921333 68920665 ID=cds.MS.gene88151.t1;Parent=MS.gene88151.t1
Gene Sequence

>MS.gene88151

ATGGCTAGAAGTAAGGTTAAACTCGCCTTCATTGTCAATGATGCTGCAAGGAAAGCAACATACAAAAAAAGAACAAAGGGTCTATTGAAAAAGGTTGCTGAACTTAGCACCCTCTGTGGCATTGAAGCCTGTGCCATAGTGTATGGCTCTTATGAGCCTCAACCTGAGATTTGGCCTTCACCAGAGGGGGTCCAATCTGTGCTCTCAAAATTCATGACGATGCACGAGTTTCAAAAGTGCAAGAAAATGCTGAACCAAGAGACTTTCTTGACACAAAGGATTCTGAAGGCTGAAGAGAAATTGATGAAACAAAGAAAAGAAAATAGGGAGCAAGAGATGACAATACTCATGTCTCATTGTCTTAATGAAGGTAGAGTTGTGCACGACAATATGTCAACGGTTGATTTGAGTTATCTTACTTGGTTGATTGACCATAATTTGAAGGACATTGGTAGACGTTTGGCGGCAACAGTGCCACCACCACCTCCAACAGCATCTAGGAGTGAGGAAATGGGAATTGTGGGTCATGGCCATGTTGGGATACCTATTAATGATGATGAAATTATGCAAAGTCAATTATTGATGGACTCAATGGTGGCTGGTAATGTGCACGAGACAGTGCCCTTTGGTGAGATCAATTATGGAGTTTGGCCTGATTTACTACCTTGA
Protein sequence

>MS.gene88151.t1

MARSKVKLAFIVNDAARKATYKKRTKGLLKKVAELSTLCGIEACAIVYGSYEPQPEIWPSPEGVQSVLSKFMTMHEFQKCKKMLNQETFLTQRILKAEEKLMKQRKENREQEMTILMSHCLNEGRVVHDNMSTVDLSYLTWLIDHNLKDIGRRLAATVPPPPPTASRSEEMGIVGHGHVGIPINDDEIMQSQLLMDSMVAGNVHETVPFGEINYGVWPDLLP