AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene88155

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene88155.t1 MTR_4g031910 72.222 234 48 2 1 222 1 229 1.26e-112 322
MS.gene88155.t1 MTR_4g032290 69.672 244 39 2 1 222 1 231 7.53e-108 310
MS.gene88155.t1 MTR_4g032620 57.143 231 90 1 1 222 1 231 1.78e-95 278
MS.gene88155.t1 MTR_8g036130 57.490 247 80 4 1 222 1 247 1.21e-90 267
MS.gene88155.t1 MTR_4g032260 79.618 157 32 0 1 157 1 157 1.17e-80 238
MS.gene88155.t1 MTR_3g065100 47.718 241 101 4 1 219 1 238 4.44e-64 199
MS.gene88155.t1 MTR_3g466980 45.872 218 104 3 1 214 1 208 4.48e-62 193
MS.gene88155.t1 MTR_2g035610 72.131 122 34 0 1 122 1 122 6.06e-52 164
MS.gene88155.t1 MTR_4g032270 76.042 96 15 1 135 222 36 131 4.34e-45 147
MS.gene88155.t1 MTR_3g466830 48.649 148 75 1 3 150 2 148 8.12e-45 147
MS.gene88155.t1 MTR_5g075380 47.020 151 79 1 1 151 1 150 1.05e-44 147
MS.gene88155.t1 MTR_4g063790 44.156 154 86 0 1 154 1 154 9.59e-44 145
MS.gene88155.t1 MTR_3g467080 41.667 156 91 0 1 156 1 156 7.66e-42 140
MS.gene88155.t1 MTR_3g466890 45.946 148 79 1 3 150 2 148 1.12e-40 137
MS.gene88155.t1 MTR_5g047580 44.231 156 85 2 1 156 1 154 2.51e-40 135
MS.gene88155.t1 MTR_3g031100 33.929 224 136 4 1 219 1 217 1.93e-39 136
MS.gene88155.t1 MTR_2g016210 38.418 177 108 1 1 176 1 177 1.26e-38 134
MS.gene88155.t1 MTR_1g077390 41.026 156 90 2 1 156 1 154 2.98e-37 128
MS.gene88155.t1 MTR_7g011950 42.949 156 87 2 1 156 1 154 6.18e-35 122
MS.gene88155.t1 MTR_1g090783 40.816 147 85 2 3 149 2 146 1.14e-34 121
MS.gene88155.t1 MTR_1g077320 42.949 156 87 2 1 156 1 154 5.33e-34 120
MS.gene88155.t1 MTR_3g465410 45.082 122 67 0 29 150 1 122 8.05e-34 119
MS.gene88155.t1 MTR_1g090710 38.312 154 94 1 1 154 1 153 1.53e-33 118
MS.gene88155.t1 MTR_1g090697 40.000 150 88 2 1 150 1 148 2.01e-33 118
MS.gene88155.t1 MTR_4g028720 42.308 156 88 2 1 156 1 154 3.25e-33 117
MS.gene88155.t1 MTR_4g028800 77.143 70 14 1 1 70 1 68 2.09e-32 113
MS.gene88155.t1 MTR_1g077300 42.949 156 85 2 1 156 1 152 2.59e-32 115
MS.gene88155.t1 MTR_1g084950 42.949 156 87 2 1 156 1 154 4.79e-32 114
MS.gene88155.t1 MTR_7g055800 52.586 116 27 2 1 116 1 88 1.28e-30 108
MS.gene88155.t1 MTR_3g031240 34.810 158 99 2 1 156 1 156 1.74e-29 108
MS.gene88155.t1 MTR_3g466900 40.650 123 72 1 32 154 2 123 6.35e-29 105
MS.gene88155.t1 MTR_7g055790 53.153 111 34 1 1 111 59 151 1.25e-26 100
MS.gene88155.t1 MTR_3g466930 38.211 123 75 1 32 154 2 123 1.71e-26 99.8
MS.gene88155.t1 MTR_7g055940 50.943 106 24 2 1 106 1 78 3.12e-25 94.7
MS.gene88155.t1 MTR_2g035580 35.669 157 100 1 1 157 1 156 1.47e-24 95.5
MS.gene88155.t1 MTR_4g019670 40.000 125 75 0 3 127 6 130 1.85e-24 101
MS.gene88155.t1 MTR_7g106510 37.795 127 79 0 3 129 6 132 7.67e-24 99.4
MS.gene88155.t1 MTR_5g047560 41.026 117 52 2 1 117 1 100 6.03e-22 88.2
MS.gene88155.t1 MTR_3g093900 31.527 203 126 4 3 196 6 204 1.62e-21 92.8
MS.gene88155.t1 MTR_2g035590 58.025 81 34 0 74 154 1 81 2.47e-19 80.1
MS.gene88155.t1 MTR_4g084780 39.450 109 50 2 76 168 3 111 8.07e-14 67.0
MS.gene88155.t1 MTR_6g018920 35.398 113 66 4 1 107 1 112 1.60e-13 68.9
MS.gene88155.t1 MTR_1g114730 31.783 129 76 4 1 123 1 123 1.96e-13 68.9
MS.gene88155.t1 MTR_6g005450 35.398 113 66 4 1 107 1 112 2.14e-13 68.6
MS.gene88155.t1 MTR_6g005440 36.283 113 65 4 1 107 1 112 2.87e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene88155.t1 AT5G48670 54.839 155 67 2 1 154 1 153 4.36e-46 155
MS.gene88155.t1 AT5G26630 42.439 205 107 2 1 195 1 204 1.19e-41 141
MS.gene88155.t1 AT1G65300 44.737 152 83 1 3 154 2 152 5.27e-41 141
MS.gene88155.t1 AT1G65330 45.395 152 82 1 3 154 2 152 9.51e-40 138
MS.gene88155.t1 AT5G26650 45.000 140 76 1 5 144 3 141 1.63e-36 132
MS.gene88155.t1 AT5G27960 45.926 135 72 1 5 139 3 136 2.86e-36 130
MS.gene88155.t1 AT1G31630 39.865 148 87 2 3 150 2 147 1.55e-35 129
MS.gene88155.t1 AT2G28700 38.636 176 101 3 1 174 1 171 6.19e-34 124
MS.gene88155.t1 AT3G05860 37.267 161 100 1 1 161 1 160 4.90e-33 119
MS.gene88155.t1 AT5G27810 55.914 93 41 0 29 121 1 93 2.19e-32 114
MS.gene88155.t1 AT3G05860 37.267 161 100 1 1 161 1 160 2.20e-32 118
MS.gene88155.t1 AT3G05860 37.267 161 100 1 1 161 1 160 3.12e-32 118
MS.gene88155.t1 AT1G31640 39.189 148 88 2 3 150 2 147 4.46e-32 122
MS.gene88155.t1 AT5G26580 36.111 144 77 2 1 144 1 129 4.89e-25 101
MS.gene88155.t1 AT1G22590 30.247 162 109 1 1 162 1 158 2.22e-24 95.1
MS.gene88155.t1 AT5G06500 33.758 157 102 2 1 157 1 155 1.95e-21 89.4
MS.gene88155.t1 AT2G40210 40.157 127 75 1 1 127 1 126 2.24e-19 85.9
MS.gene88155.t1 AT5G58890 41.593 113 55 5 6 109 6 116 3.67e-16 76.3
MS.gene88155.t1 AT5G55690 42.308 78 38 2 1 71 1 78 1.83e-13 68.2
MS.gene88155.t1 AT5G55690 42.308 78 38 2 1 71 1 78 1.83e-13 68.2
MS.gene88155.t1 AT2G15660 27.027 148 94 3 38 176 31 173 4.42e-12 64.3
MS.gene88155.t1 AT5G27944 32.727 110 62 3 24 131 41 140 8.71e-11 60.1

Find 58 sgRNAs with CRISPR-Local

Find 60 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCCTTTGGTGAGATCAATTC+TGG 0.238779 4.3:-68846071 MS.gene88155:CDS
GAGCGAAGAAATGGCAATTA+TGG 0.251384 4.3:-68846192 MS.gene88155:CDS
TACCAATGTCCTTCAAATTA+TGG 0.252012 4.3:+68846261 None:intergenic
TCCTTCAAAGGTAGTAAATC+AGG 0.298213 4.3:+68846041 None:intergenic
AAGAAAGGGTCTATTGAAAA+AGG 0.312767 4.3:-68846621 MS.gene88155:CDS
CCCTGATTTACTACCTTTGA+AGG 0.324619 4.3:-68846042 MS.gene88155:CDS
ATGAGCCTCAACCAGAGATT+TGG 0.345216 4.3:-68846541 MS.gene88155:CDS
ATCTCTGGTTGAGGCTCATA+AGG 0.350685 4.3:+68846545 None:intergenic
AAAATGTCATCACCATTAAT+AGG 0.355958 4.3:+68846149 None:intergenic
TTTGCAAAGTCAATTATTGA+TGG 0.379269 4.3:-68846129 MS.gene88155:CDS
GAAGGACATTGGTAGACATT+TGG 0.385901 4.3:-68846252 MS.gene88155:CDS
AGCGAAGAAATGGCAATTAT+GGG 0.425600 4.3:-68846191 MS.gene88155:CDS
AGCATCTAGGAGCGAAGAAA+TGG 0.428793 4.3:-68846201 MS.gene88155:CDS
TCTGGTGAAGGCCAAATCTC+TGG 0.429918 4.3:+68846530 None:intergenic
GTTGATTGACCATAATTTGA+AGG 0.445081 4.3:-68846270 MS.gene88155:CDS
CACAACCATGGCTAGAAGTA+AGG 0.461347 4.3:-68846699 None:intergenic
CTCATAAGGGCCATACACTA+TGG 0.468858 4.3:+68846559 None:intergenic
ATCATACAAGAAAAGAAGAA+AGG 0.473094 4.3:-68846636 MS.gene88155:CDS
CTTGACACAAAGGGTTCTGA+AGG 0.478789 4.3:-68846426 MS.gene88155:CDS
TCAATTATTGATGGACTCAA+TGG 0.482426 4.3:-68846120 MS.gene88155:CDS
TTGATTTGAGTTATCTTACT+TGG 0.482489 4.3:-68846292 MS.gene88155:CDS
CATGTTGGGATACCTATTAA+TGG 0.485702 4.3:-68846161 MS.gene88155:CDS
TCTTCGCTCCTAGATGCTGC+TGG 0.487238 4.3:+68846206 None:intergenic
GATGCTGCTGGAGGTGATAG+TGG 0.492236 4.3:+68846218 None:intergenic
TATCACCTCCAGCAGCATCT+AGG 0.496968 4.3:-68846214 MS.gene88155:CDS
TTATGGGTCATGGACATGTT+GGG 0.500968 4.3:-68846175 MS.gene88155:CDS
TGCAGCATCATTGACAATGA+AGG 0.502295 4.3:+68846667 None:intergenic
ATTATGGGTCATGGACATGT+TGG 0.516074 4.3:-68846176 MS.gene88155:CDS
AGAGACTTTCTTGACACAAA+GGG 0.517022 4.3:-68846435 MS.gene88155:CDS
GACCATAATTTGAAGGACAT+TGG 0.517963 4.3:-68846263 MS.gene88155:CDS
GAAATGGCAATTATGGGTCA+TGG 0.521926 4.3:-68846185 MS.gene88155:CDS
TTTGTGTCAAGAAAGTCTCT+TGG 0.525855 4.3:+68846438 None:intergenic
TCATACAAGAAAAGAAGAAA+GGG 0.526600 4.3:-68846635 MS.gene88155:CDS
TTTAACCTTACTTCTAGCCA+TGG 0.533701 4.3:+68846694 None:intergenic
GGACATTGGTAGACATTTGG+CGG 0.537707 4.3:-68846249 MS.gene88155:CDS
ATGTATCATTGTCTCAATGA+AGG 0.546520 4.3:-68846344 MS.gene88155:CDS
TCTCTGGTTGAGGCTCATAA+GGG 0.546756 4.3:+68846546 None:intergenic
CCAGAATTGATCTCACCAAA+GGG 0.554343 4.3:+68846071 None:intergenic
GTGCAAGAGACAGTGCCCTT+TGG 0.557727 4.3:-68846086 MS.gene88155:CDS
GAAGCCTGTGCCATAGTGTA+TGG 0.559714 4.3:-68846569 MS.gene88155:CDS
CCTTCAAAGGTAGTAAATCA+GGG 0.575295 4.3:+68846042 None:intergenic
GAGATTTGGCCTTCACCAGA+GGG 0.575380 4.3:-68846527 MS.gene88155:CDS
GAAGGCCAAATCTCTGGTTG+AGG 0.576852 4.3:+68846536 None:intergenic
TGTGCACAACAATATTTCAA+CGG 0.585275 4.3:-68846315 MS.gene88155:CDS
TCCAGAATTGATCTCACCAA+AGG 0.590517 4.3:+68846070 None:intergenic
ATTGAGAAAAGATCCTTCAA+AGG 0.601093 4.3:+68846029 None:intergenic
ACAGGCTTCAATGCCACAGA+GGG 0.601811 4.3:+68846583 None:intergenic
GATTTGGCCTTCACCAGAGG+GGG 0.601928 4.3:-68846525 MS.gene88155:CDS
TTGATGGACTCAATGGTGGC+TGG 0.607204 4.3:-68846113 MS.gene88155:CDS
AAGAGACTTTCTTGACACAA+AGG 0.607804 4.3:-68846436 MS.gene88155:CDS
AGAGATTTGGCCTTCACCAG+AGG 0.616752 4.3:-68846528 MS.gene88155:CDS
AGGGCCATACACTATGGCAC+AGG 0.641363 4.3:+68846565 None:intergenic
TCATTGTCAATGATGCTGCA+AGG 0.652548 4.3:-68846664 MS.gene88155:CDS
ATTATTGATGGACTCAATGG+TGG 0.666274 4.3:-68846117 MS.gene88155:CDS
TCGCTCCTAGATGCTGCTGG+AGG 0.676090 4.3:+68846209 None:intergenic
GCTGAACTTAGCACCCTCTG+TGG 0.677042 4.3:-68846596 MS.gene88155:CDS
CACAGGCTTCAATGCCACAG+AGG 0.696692 4.3:+68846582 None:intergenic
AGATTTGGCCTTCACCAGAG+GGG 0.703414 4.3:-68846526 MS.gene88155:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AAAATGTCATCACCATTAAT+AGG + chr4.3:68846571-68846590 None:intergenic 25.0%
! ATCATACAAGAAAAGAAGAA+AGG - chr4.3:68846081-68846100 MS.gene88155:CDS 25.0%
! TCATACAAGAAAAGAAGAAA+GGG - chr4.3:68846082-68846101 MS.gene88155:CDS 25.0%
! TTTGCAAAGTCAATTATTGA+TGG - chr4.3:68846588-68846607 MS.gene88155:CDS 25.0%
!! TTGATTTGAGTTATCTTACT+TGG - chr4.3:68846425-68846444 MS.gene88155:CDS 25.0%
AAGAAAGGGTCTATTGAAAA+AGG - chr4.3:68846096-68846115 MS.gene88155:CDS 30.0%
ATGTATCATTGTCTCAATGA+AGG - chr4.3:68846373-68846392 MS.gene88155:CDS 30.0%
ATTGAGAAAAGATCCTTCAA+AGG + chr4.3:68846691-68846710 None:intergenic 30.0%
GTTGATTGACCATAATTTGA+AGG - chr4.3:68846447-68846466 MS.gene88155:CDS 30.0%
TACCAATGTCCTTCAAATTA+TGG + chr4.3:68846459-68846478 None:intergenic 30.0%
TGTGCACAACAATATTTCAA+CGG - chr4.3:68846402-68846421 MS.gene88155:CDS 30.0%
!! TCAATTATTGATGGACTCAA+TGG - chr4.3:68846597-68846616 MS.gene88155:CDS 30.0%
AAGAGACTTTCTTGACACAA+AGG - chr4.3:68846281-68846300 MS.gene88155:CDS 35.0%
AGAGACTTTCTTGACACAAA+GGG - chr4.3:68846282-68846301 MS.gene88155:CDS 35.0%
AGCGAAGAAATGGCAATTAT+GGG - chr4.3:68846526-68846545 MS.gene88155:CDS 35.0%
CATGTTGGGATACCTATTAA+TGG - chr4.3:68846556-68846575 MS.gene88155:CDS 35.0%
CCTTCAAAGGTAGTAAATCA+GGG + chr4.3:68846678-68846697 None:intergenic 35.0%
GACCATAATTTGAAGGACAT+TGG - chr4.3:68846454-68846473 MS.gene88155:CDS 35.0%
TCCTTCAAAGGTAGTAAATC+AGG + chr4.3:68846679-68846698 None:intergenic 35.0%
TTTGTGTCAAGAAAGTCTCT+TGG + chr4.3:68846282-68846301 None:intergenic 35.0%
!! ATTATTGATGGACTCAATGG+TGG - chr4.3:68846600-68846619 MS.gene88155:CDS 35.0%
ATTATGGGTCATGGACATGT+TGG - chr4.3:68846541-68846560 MS.gene88155:CDS 40.0%
CCAGAATTGATCTCACCAAA+GGG + chr4.3:68846649-68846668 None:intergenic 40.0%
CCCTGATTTACTACCTTTGA+AGG - chr4.3:68846675-68846694 MS.gene88155:CDS 40.0%
GAAATGGCAATTATGGGTCA+TGG - chr4.3:68846532-68846551 MS.gene88155:CDS 40.0%
GAAGGACATTGGTAGACATT+TGG - chr4.3:68846465-68846484 MS.gene88155:CDS 40.0%
GAGCGAAGAAATGGCAATTA+TGG - chr4.3:68846525-68846544 MS.gene88155:CDS 40.0%
TCATTGTCAATGATGCTGCA+AGG - chr4.3:68846053-68846072 MS.gene88155:CDS 40.0%
TCCAGAATTGATCTCACCAA+AGG + chr4.3:68846650-68846669 None:intergenic 40.0%
TGCAGCATCATTGACAATGA+AGG + chr4.3:68846053-68846072 None:intergenic 40.0%
TTATGGGTCATGGACATGTT+GGG - chr4.3:68846542-68846561 MS.gene88155:CDS 40.0%
!! CGTCATGAATTTTGAGAGCA+CGG + chr4.3:68846224-68846243 None:intergenic 40.0%
!! TGAATTTTGAGAGCACGGAT+TGG + chr4.3:68846219-68846238 None:intergenic 40.0%
AGCATCTAGGAGCGAAGAAA+TGG - chr4.3:68846516-68846535 MS.gene88155:CDS 45.0%
ATCTCTGGTTGAGGCTCATA+AGG + chr4.3:68846175-68846194 None:intergenic 45.0%
ATGAGCCTCAACCAGAGATT+TGG - chr4.3:68846176-68846195 MS.gene88155:CDS 45.0%
CCCTTTGGTGAGATCAATTC+TGG - chr4.3:68846646-68846665 MS.gene88155:CDS 45.0%
CTCATAAGGGCCATACACTA+TGG + chr4.3:68846161-68846180 None:intergenic 45.0%
GGACATTGGTAGACATTTGG+CGG - chr4.3:68846468-68846487 MS.gene88155:CDS 45.0%
TCTCTGGTTGAGGCTCATAA+GGG + chr4.3:68846174-68846193 None:intergenic 45.0%
! CTTGACACAAAGGGTTCTGA+AGG - chr4.3:68846291-68846310 MS.gene88155:CDS 45.0%
ACAGGCTTCAATGCCACAGA+GGG + chr4.3:68846137-68846156 None:intergenic 50.0%
GAAGCCTGTGCCATAGTGTA+TGG - chr4.3:68846148-68846167 MS.gene88155:CDS 50.0%
GAAGGCCAAATCTCTGGTTG+AGG + chr4.3:68846184-68846203 None:intergenic 50.0%
TATCACCTCCAGCAGCATCT+AGG - chr4.3:68846503-68846522 MS.gene88155:CDS 50.0%
TCTGGTGAAGGCCAAATCTC+TGG + chr4.3:68846190-68846209 None:intergenic 50.0%
! AGAGATTTGGCCTTCACCAG+AGG - chr4.3:68846189-68846208 MS.gene88155:CDS 50.0%
! AGATTTGGCCTTCACCAGAG+GGG - chr4.3:68846191-68846210 MS.gene88155:CDS 50.0%
! GAGATTTGGCCTTCACCAGA+GGG - chr4.3:68846190-68846209 MS.gene88155:CDS 50.0%
!! TTGATGGACTCAATGGTGGC+TGG - chr4.3:68846604-68846623 MS.gene88155:CDS 50.0%
AGGGCCATACACTATGGCAC+AGG + chr4.3:68846155-68846174 None:intergenic 55.0%
CACAGGCTTCAATGCCACAG+AGG + chr4.3:68846138-68846157 None:intergenic 55.0%
GATGCTGCTGGAGGTGATAG+TGG + chr4.3:68846502-68846521 None:intergenic 55.0%
GCTGAACTTAGCACCCTCTG+TGG - chr4.3:68846121-68846140 MS.gene88155:CDS 55.0%
GTGCAAGAGACAGTGCCCTT+TGG - chr4.3:68846631-68846650 MS.gene88155:CDS 55.0%
TCTTCGCTCCTAGATGCTGC+TGG + chr4.3:68846514-68846533 None:intergenic 55.0%
! GATTTGGCCTTCACCAGAGG+GGG - chr4.3:68846192-68846211 MS.gene88155:CDS 55.0%
GATTGGACCCCCTCTGGTGA+AGG + chr4.3:68846202-68846221 None:intergenic 60.0%
TCGCTCCTAGATGCTGCTGG+AGG + chr4.3:68846511-68846530 None:intergenic 60.0%
!! AGCACGGATTGGACCCCCTC+TGG + chr4.3:68846208-68846227 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 68846025 68846714 68846025 ID=MS.gene88155
chr4.3 mRNA 68846025 68846714 68846025 ID=MS.gene88155.t1;Parent=MS.gene88155
chr4.3 exon 68846025 68846714 68846025 ID=MS.gene88155.t1.exon1;Parent=MS.gene88155.t1
chr4.3 CDS 68846025 68846714 68846025 ID=cds.MS.gene88155.t1;Parent=MS.gene88155.t1
Gene Sequence

>MS.gene88155

ATGGCTAGAAGTAAGGTTAAACTTGCCTTCATTGTCAATGATGCTGCAAGGAAAGAATCATACAAGAAAAGAAGAAAGGGTCTATTGAAAAAGGTTGCTGAACTTAGCACCCTCTGTGGCATTGAAGCCTGTGCCATAGTGTATGGCCCTTATGAGCCTCAACCAGAGATTTGGCCTTCACCAGAGGGGGTCCAATCCGTGCTCTCAAAATTCATGACGATTCACGAGTTTCAAAAGTGCAAGAAAATGATGAACCAAGAGACTTTCTTGACACAAAGGGTTCTGAAGGCTGAAGAGAAATTGAAGAAACAAAGAAAAGAAAATAGAGAGCAAGAGATGACAATACTCATGTATCATTGTCTCAATGAAGGTAGAGTTGTGCACAACAATATTTCAACGGTTGATTTGAGTTATCTTACTTGGTTGATTGACCATAATTTGAAGGACATTGGTAGACATTTGGCGGCAACAGTGCCACTATCACCTCCAGCAGCATCTAGGAGCGAAGAAATGGCAATTATGGGTCATGGACATGTTGGGATACCTATTAATGGTGATGACATTTTGCAAAGTCAATTATTGATGGACTCAATGGTGGCTGGTAATGTGCAAGAGACAGTGCCCTTTGGTGAGATCAATTCTGGAGTTTGCCCTGATTTACTACCTTTGAAGGATCTTTTCTCAATTTAG
Protein sequence

>MS.gene88155.t1

MARSKVKLAFIVNDAARKESYKKRRKGLLKKVAELSTLCGIEACAIVYGPYEPQPEIWPSPEGVQSVLSKFMTIHEFQKCKKMMNQETFLTQRVLKAEEKLKKQRKENREQEMTILMYHCLNEGRVVHNNISTVDLSYLTWLIDHNLKDIGRHLAATVPLSPPAASRSEEMAIMGHGHVGIPINGDDILQSQLLMDSMVAGNVQETVPFGEINSGVCPDLLPLKDLFSI