Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88160.t1 | RHN76722.1 | 55.6 | 90 | 13 | 1 | 49 | 111 | 504 | 593 | 1.20E-14 | 89.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88160.t1 | Q9FJK3 | 52.6 | 76 | 32 | 1 | 4 | 79 | 3 | 74 | 1.4e-11 | 70.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88160.t1 | G7I3H5 | 55.6 | 90 | 13 | 1 | 49 | 111 | 920 | 1009 | 8.5e-15 | 89.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene88160.t1 | TF | MADS-M-type |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88160.t1 | MTR_1g007990 | 52.041 | 98 | 20 | 1 | 41 | 111 | 912 | 1009 | 4.19e-23 | 92.8 |
| MS.gene88160.t1 | MTR_8g036130 | 82.222 | 45 | 8 | 0 | 4 | 48 | 3 | 47 | 7.00e-18 | 76.3 |
| MS.gene88160.t1 | MTR_4g032620 | 62.712 | 59 | 15 | 2 | 1 | 53 | 1 | 58 | 1.02e-16 | 72.8 |
| MS.gene88160.t1 | MTR_1g090783 | 56.452 | 62 | 23 | 1 | 4 | 65 | 2 | 59 | 1.84e-16 | 70.5 |
| MS.gene88160.t1 | MTR_1g090697 | 48.718 | 78 | 36 | 1 | 4 | 81 | 3 | 76 | 7.48e-16 | 68.9 |
| MS.gene88160.t1 | MTR_5g047580 | 56.452 | 62 | 23 | 1 | 4 | 65 | 3 | 60 | 1.48e-15 | 68.2 |
| MS.gene88160.t1 | MTR_3g466830 | 64.000 | 50 | 18 | 0 | 4 | 53 | 2 | 51 | 3.87e-15 | 67.4 |
| MS.gene88160.t1 | MTR_4g028800 | 76.744 | 43 | 8 | 1 | 6 | 48 | 5 | 45 | 6.99e-15 | 64.3 |
| MS.gene88160.t1 | MTR_1g077390 | 42.529 | 87 | 46 | 1 | 4 | 90 | 3 | 85 | 1.57e-14 | 65.5 |
| MS.gene88160.t1 | MTR_3g466890 | 71.111 | 45 | 13 | 0 | 4 | 48 | 2 | 46 | 2.94e-14 | 65.1 |
| MS.gene88160.t1 | MTR_2g016210 | 69.767 | 43 | 13 | 0 | 6 | 48 | 5 | 47 | 3.48e-14 | 66.2 |
| MS.gene88160.t1 | MTR_3g466980 | 64.444 | 45 | 16 | 0 | 4 | 48 | 3 | 47 | 1.13e-13 | 64.7 |
| MS.gene88160.t1 | MTR_5g075380 | 65.116 | 43 | 15 | 0 | 6 | 48 | 5 | 47 | 7.27e-13 | 61.2 |
| MS.gene88160.t1 | MTR_4g031910 | 84.444 | 45 | 7 | 0 | 4 | 48 | 3 | 47 | 1.16e-12 | 62.0 |
| MS.gene88160.t1 | MTR_3g031240 | 40.506 | 79 | 42 | 2 | 1 | 79 | 1 | 74 | 1.27e-12 | 61.2 |
| MS.gene88160.t1 | MTR_4g063790 | 52.083 | 48 | 23 | 0 | 4 | 51 | 3 | 50 | 1.94e-12 | 60.5 |
| MS.gene88160.t1 | MTR_2g035610 | 80.435 | 46 | 9 | 0 | 4 | 49 | 3 | 48 | 3.36e-12 | 58.9 |
| MS.gene88160.t1 | MTR_1g090710 | 50.000 | 64 | 27 | 2 | 1 | 64 | 1 | 59 | 4.88e-12 | 59.3 |
| MS.gene88160.t1 | MTR_3g065100 | 77.778 | 45 | 10 | 0 | 4 | 48 | 3 | 47 | 1.86e-11 | 58.9 |
| MS.gene88160.t1 | MTR_3g031100 | 43.939 | 66 | 32 | 2 | 1 | 66 | 1 | 61 | 8.50e-11 | 57.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88160.t1 | AT3G05860 | 42.169 | 83 | 41 | 2 | 4 | 80 | 3 | 84 | 4.24e-15 | 68.2 |
| MS.gene88160.t1 | AT3G05860 | 42.169 | 83 | 41 | 2 | 4 | 80 | 3 | 84 | 7.23e-15 | 68.2 |
| MS.gene88160.t1 | AT3G05860 | 42.169 | 83 | 41 | 2 | 4 | 80 | 3 | 84 | 9.22e-15 | 68.2 |
| MS.gene88160.t1 | AT1G22590 | 36.975 | 119 | 61 | 5 | 1 | 110 | 1 | 114 | 3.97e-13 | 62.0 |
| MS.gene88160.t1 | AT1G65330 | 55.556 | 45 | 20 | 0 | 4 | 48 | 2 | 46 | 1.95e-11 | 59.3 |
Find 23 sgRNAs with CRISPR-Local
Find 31 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCAACATTTCCGTAGCTTC+TGG | 0.330276 | 4.3:+68786324 | None:intergenic |
| TTACTACTGAAGATTGAAAA+AGG | 0.333620 | 4.3:-68786279 | MS.gene88160:intron |
| GAAGAAGAGTCTATTCAAAA+AGG | 0.366702 | 4.3:-68786549 | MS.gene88160:CDS |
| AGCATAGGTGTCTCAATCTC+TGG | 0.414241 | 4.3:+68786135 | None:intergenic |
| GGAAAGCAACATACAAGAAA+AGG | 0.449202 | 4.3:-68786571 | MS.gene88160:CDS |
| AACCTTACTTCTTCTACCCA+TGG | 0.464315 | 4.3:+68786625 | None:intergenic |
| AGATACAACACCGAAGCCTT+TGG | 0.487832 | 4.3:-68786178 | MS.gene88160:CDS |
| AACCATGGGTAGAAGAAGTA+AGG | 0.501192 | 4.3:-68786627 | None:intergenic |
| AGCAGCATCATTGACAATGA+AGG | 0.509472 | 4.3:+68786595 | None:intergenic |
| GAGACACCTATGCTAGACTT+AGG | 0.514532 | 4.3:-68786126 | MS.gene88160:CDS |
| TAGCACCCTATGTGGTATTG+AGG | 0.514801 | 4.3:-68786516 | MS.gene88160:CDS |
| GATGCCTTGACCAAAGGCTT+CGG | 0.538647 | 4.3:+68786168 | None:intergenic |
| TCTAACGATGCCTTGACCAA+AGG | 0.554955 | 4.3:+68786162 | None:intergenic |
| ACAAGCCTCAATACCACATA+GGG | 0.561401 | 4.3:+68786511 | None:intergenic |
| GGATATTCCTCCAGAAGCTA+CGG | 0.564585 | 4.3:-68786334 | MS.gene88160:CDS |
| AACATTTCCGTAGCTTCTGG+AGG | 0.585851 | 4.3:+68786327 | None:intergenic |
| AACACCGAAGCCTTTGGTCA+AGG | 0.591331 | 4.3:-68786172 | MS.gene88160:CDS |
| TCATTGTCAATGATGCTGCT+AGG | 0.603132 | 4.3:-68786592 | MS.gene88160:CDS |
| CAATCTCCTAAGTCTAGCAT+AGG | 0.607643 | 4.3:+68786120 | None:intergenic |
| GTTGAACTTAGCACCCTATG+TGG | 0.618465 | 4.3:-68786524 | MS.gene88160:CDS |
| CACAAGCCTCAATACCACAT+AGG | 0.619824 | 4.3:+68786510 | None:intergenic |
| ATATCCATCTTGTTCCCACA+AGG | 0.643932 | 4.3:+68786351 | None:intergenic |
| CCACAAGGAAAATATATGCG+AGG | 0.655668 | 4.3:+68786366 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAAAAAACAGAATAGTTAGA+TGG | + | chr4.3:68786351-68786370 | None:intergenic | 20.0% |
| !! | TTCGTATTTGTATTATGTAT+AGG | - | chr4.3:68786308-68786327 | MS.gene88160:CDS | 20.0% |
| !!! | ATCTAACTATTCTGTTTTTT+AGG | - | chr4.3:68786350-68786369 | MS.gene88160:CDS | 20.0% |
| ! | TTACTACTGAAGATTGAAAA+AGG | - | chr4.3:68786463-68786482 | MS.gene88160:intron | 25.0% |
| !! | GTATTATGTATAGGTTTTTG+AGG | - | chr4.3:68786317-68786336 | MS.gene88160:CDS | 25.0% |
| GAAGAAGAGTCTATTCAAAA+AGG | - | chr4.3:68786193-68786212 | MS.gene88160:intron | 30.0% | |
| GAAATGCAAAACTCTCGTAA+AGG | + | chr4.3:68786271-68786290 | None:intergenic | 35.0% | |
| GGAAAGCAACATACAAGAAA+AGG | - | chr4.3:68786171-68786190 | MS.gene88160:CDS | 35.0% | |
| ! | CTCGCATATATTTTCCTTGT+GGG | - | chr4.3:68786377-68786396 | MS.gene88160:intron | 35.0% |
| ACAAGCCTCAATACCACATA+GGG | + | chr4.3:68786234-68786253 | None:intergenic | 40.0% | |
| AGCAGCATCATTGACAATGA+AGG | + | chr4.3:68786150-68786169 | None:intergenic | 40.0% | |
| ATATCCATCTTGTTCCCACA+AGG | + | chr4.3:68786394-68786413 | None:intergenic | 40.0% | |
| CAATCTCCTAAGTCTAGCAT+AGG | + | chr4.3:68786625-68786644 | None:intergenic | 40.0% | |
| CCACAAGGAAAATATATGCG+AGG | + | chr4.3:68786379-68786398 | None:intergenic | 40.0% | |
| TCATTGTCAATGATGCTGCT+AGG | - | chr4.3:68786150-68786169 | MS.gene88160:CDS | 40.0% | |
| TTCAACATTTCCGTAGCTTC+TGG | + | chr4.3:68786421-68786440 | None:intergenic | 40.0% | |
| ! | CCTCGCATATATTTTCCTTG+TGG | - | chr4.3:68786376-68786395 | MS.gene88160:intron | 40.0% |
| ! | TTTTCCTTGTGGGAACAAGA+TGG | - | chr4.3:68786387-68786406 | MS.gene88160:intron | 40.0% |
| AACATTTCCGTAGCTTCTGG+AGG | + | chr4.3:68786418-68786437 | None:intergenic | 45.0% | |
| AGATACAACACCGAAGCCTT+TGG | - | chr4.3:68786564-68786583 | MS.gene88160:CDS | 45.0% | |
| AGCATAGGTGTCTCAATCTC+TGG | + | chr4.3:68786610-68786629 | None:intergenic | 45.0% | |
| CACAAGCCTCAATACCACAT+AGG | + | chr4.3:68786235-68786254 | None:intergenic | 45.0% | |
| CTCGTAAAGGCCATACACTA+TGG | + | chr4.3:68786258-68786277 | None:intergenic | 45.0% | |
| GAGACACCTATGCTAGACTT+AGG | - | chr4.3:68786616-68786635 | MS.gene88160:CDS | 45.0% | |
| GGATATTCCTCCAGAAGCTA+CGG | - | chr4.3:68786408-68786427 | MS.gene88160:intron | 45.0% | |
| GTTGAACTTAGCACCCTATG+TGG | - | chr4.3:68786218-68786237 | MS.gene88160:intron | 45.0% | |
| TAGCACCCTATGTGGTATTG+AGG | - | chr4.3:68786226-68786245 | MS.gene88160:intron | 45.0% | |
| TCTAACGATGCCTTGACCAA+AGG | + | chr4.3:68786583-68786602 | None:intergenic | 45.0% | |
| AACACCGAAGCCTTTGGTCA+AGG | - | chr4.3:68786570-68786589 | MS.gene88160:CDS | 50.0% | |
| GAGGCTTGTGCCATAGTGTA+TGG | - | chr4.3:68786245-68786264 | MS.gene88160:intron | 50.0% | |
| GATGCCTTGACCAAAGGCTT+CGG | + | chr4.3:68786577-68786596 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 68786119 | 68786645 | 68786119 | ID=MS.gene88160 |
| chr4.3 | mRNA | 68786119 | 68786645 | 68786119 | ID=MS.gene88160.t1;Parent=MS.gene88160 |
| chr4.3 | exon | 68786503 | 68786645 | 68786503 | ID=MS.gene88160.t1.exon1;Parent=MS.gene88160.t1 |
| chr4.3 | CDS | 68786503 | 68786645 | 68786503 | ID=cds.MS.gene88160.t1;Parent=MS.gene88160.t1 |
| chr4.3 | exon | 68786280 | 68786392 | 68786280 | ID=MS.gene88160.t1.exon2;Parent=MS.gene88160.t1 |
| chr4.3 | CDS | 68786280 | 68786392 | 68786280 | ID=cds.MS.gene88160.t1;Parent=MS.gene88160.t1 |
| chr4.3 | exon | 68786119 | 68786198 | 68786119 | ID=MS.gene88160.t1.exon3;Parent=MS.gene88160.t1 |
| chr4.3 | CDS | 68786119 | 68786198 | 68786119 | ID=cds.MS.gene88160.t1;Parent=MS.gene88160.t1 |
| Gene Sequence |
| Protein sequence |
