Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88299.t1 | XP_024636235.1 | 94.7 | 243 | 13 | 0 | 1 | 243 | 1 | 243 | 1.30E-111 | 412.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88299.t1 | Q9M202 | 27.8 | 194 | 133 | 4 | 9 | 196 | 6 | 198 | 1.6e-09 | 64.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88299.t1 | A0A396I2K7 | 66.9 | 245 | 75 | 1 | 1 | 239 | 1 | 245 | 7.2e-67 | 263.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene88299.t1 | TF | C2H2 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88299.t1 | MTR_4g036615 | 77.885 | 104 | 23 | 0 | 78 | 181 | 1 | 104 | 9.50e-55 | 171 |
| MS.gene88299.t1 | MTR_3g408380 | 28.302 | 265 | 146 | 7 | 3 | 229 | 2 | 260 | 1.34e-21 | 90.9 |
| MS.gene88299.t1 | MTR_3g408420 | 26.165 | 279 | 138 | 7 | 7 | 232 | 6 | 269 | 4.47e-18 | 81.3 |
| MS.gene88299.t1 | MTR_3g008310 | 26.415 | 265 | 157 | 7 | 3 | 229 | 2 | 266 | 5.00e-16 | 75.9 |
| MS.gene88299.t1 | MTR_4g100860 | 59.322 | 59 | 22 | 1 | 173 | 229 | 210 | 268 | 2.94e-13 | 68.6 |
| MS.gene88299.t1 | MTR_0160s0030 | 40.741 | 81 | 38 | 1 | 170 | 240 | 239 | 319 | 9.03e-13 | 67.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88299.t1 | AT3G60580 | 30.400 | 250 | 136 | 7 | 9 | 229 | 6 | 246 | 6.03e-18 | 81.3 |
| MS.gene88299.t1 | AT1G02030 | 27.132 | 258 | 132 | 6 | 9 | 236 | 7 | 238 | 3.94e-13 | 67.4 |
| MS.gene88299.t1 | AT5G61470 | 41.096 | 73 | 31 | 2 | 168 | 229 | 225 | 296 | 3.84e-12 | 65.1 |
Find 68 sgRNAs with CRISPR-Local
Find 72 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCTGTGTTTGTTGTATTTC+GGG | 0.187551 | 4.4:-66782132 | None:intergenic |
| ATTCTGTGTTTGTTGTATTT+CGG | 0.237824 | 4.4:-66782133 | None:intergenic |
| GCCGCAATTGGATCTCTTGC+GGG | 0.303465 | 4.4:-66782241 | None:intergenic |
| AATTGGATCTCTTGCGGGTT+GGG | 0.313326 | 4.4:-66782236 | None:intergenic |
| ATCCAGGTGTTCTGATGTTA+TGG | 0.324938 | 4.4:+66782307 | MS.gene88299:CDS |
| TGAGTATTAGTTTCAAGCTT+TGG | 0.337317 | 4.4:-66782042 | None:intergenic |
| CAGCCGCAGCATTAACTTCT+CGG | 0.371873 | 4.4:-66782263 | None:intergenic |
| CAATTGGATCTCTTGCGGGT+TGG | 0.402481 | 4.4:-66782237 | None:intergenic |
| ATTAACTTCTCGGCCGCAAT+TGG | 0.433182 | 4.4:-66782253 | None:intergenic |
| TACTCAAGCTGTAGACAATT+CGG | 0.436960 | 4.4:+66782059 | MS.gene88299:CDS |
| ATTTGTGGGAAGTGGTTCTC+CGG | 0.453707 | 4.4:+66781964 | MS.gene88299:CDS |
| GTTTCAAGCTTTGGAGCAAT+CGG | 0.454553 | 4.4:-66782033 | None:intergenic |
| TTCCGATCTTATCAGGCTCT+TGG | 0.457389 | 4.4:+66782471 | MS.gene88299:CDS |
| TTTACAAAGATGCGCAGAAA+AGG | 0.459521 | 4.4:+66782334 | MS.gene88299:CDS |
| TACAGAATCACAAATCAATA+CGG | 0.462697 | 4.4:-66782657 | None:intergenic |
| TTCTCCGGTGGGAAAGCCCT+TGG | 0.463478 | 4.4:+66781979 | MS.gene88299:CDS |
| GGAAAGCCTGTGGGATTTGT+GGG | 0.467351 | 4.4:+66781950 | MS.gene88299:CDS |
| GGGAAAGCCTGTGGGATTTG+TGG | 0.468727 | 4.4:+66781949 | MS.gene88299:CDS |
| CGATCTTATCAGGCTCTTGG+TGG | 0.468794 | 4.4:+66782474 | MS.gene88299:CDS |
| TTGCAATTTGAGCAGTGAAA+AGG | 0.471865 | 4.4:-66782549 | None:intergenic |
| GCCTCCAAGGGCTTTCCCAC+CGG | 0.472807 | 4.4:-66781983 | None:intergenic |
| CCCCATAACATCAGAACACC+TGG | 0.474579 | 4.4:-66782309 | None:intergenic |
| AGATCTCATGTGGCCTCCAA+GGG | 0.485485 | 4.4:-66781995 | None:intergenic |
| GGCCGCAATTGGATCTCTTG+CGG | 0.489489 | 4.4:-66782242 | None:intergenic |
| TCCAGGTGTTCTGATGTTAT+GGG | 0.494593 | 4.4:+66782308 | MS.gene88299:CDS |
| TCCGGTGGGAAAGCCCTTGG+AGG | 0.494800 | 4.4:+66781982 | MS.gene88299:CDS |
| TTCTCTGTTTGAGCTTGCAG+AGG | 0.496954 | 4.4:-66782187 | None:intergenic |
| TCTGTTTGAGCTTGCAGAGG+AGG | 0.498454 | 4.4:-66782184 | None:intergenic |
| TTACAAAGATGCGCAGAAAA+GGG | 0.499625 | 4.4:+66782335 | MS.gene88299:CDS |
| TTCAAGTCTGCTCAGGCACT+CGG | 0.500253 | 4.4:+66782579 | MS.gene88299:CDS |
| AACGATGGCGAAAGCAGTAC+AGG | 0.507873 | 4.4:+66782387 | MS.gene88299:CDS |
| AGCAAATTGATTTCGACGAT+GGG | 0.509489 | 4.4:-66782091 | None:intergenic |
| GCACCTCTCACAGTTGAACC+TGG | 0.509618 | 4.4:-66782439 | None:intergenic |
| ATTTCGACGATGGGTTGAAT+CGG | 0.510947 | 4.4:-66782082 | None:intergenic |
| GAGATCTCATGTGGCCTCCA+AGG | 0.514030 | 4.4:-66781996 | None:intergenic |
| GAAGATATGTCAAGAGATCC+AGG | 0.516117 | 4.4:+66782291 | MS.gene88299:CDS |
| TGTGGGAAGTGGTTCTCCGG+TGG | 0.517822 | 4.4:+66781967 | MS.gene88299:CDS |
| ACTTTGAAATTCCTTAGCAC+TGG | 0.521740 | 4.4:-66782625 | None:intergenic |
| CAAAGTGTTCAAGTCTGCTC+AGG | 0.524975 | 4.4:+66782572 | MS.gene88299:CDS |
| CACAAGCAGACTCTGAAGCC+AGG | 0.539423 | 4.4:+66782421 | MS.gene88299:CDS |
| AAGCAAATTGATTTCGACGA+TGG | 0.551421 | 4.4:-66782092 | None:intergenic |
| GTGTTTGTTGTATTTCGGGA+AGG | 0.552910 | 4.4:-66782128 | None:intergenic |
| CCTGTGGGATTTGTGGGAAG+TGG | 0.558125 | 4.4:+66781956 | MS.gene88299:CDS |
| GTGGGAAGTGGTTCTCCGGT+GGG | 0.561353 | 4.4:+66781968 | MS.gene88299:CDS |
| GATCTTATCAGGCTCTTGGT+GGG | 0.571526 | 4.4:+66782475 | MS.gene88299:CDS |
| GGTACACTCTTCCAGTGCTA+AGG | 0.575791 | 4.4:+66782614 | MS.gene88299:CDS |
| GCAACTATGGAGAAGAGCGA+TGG | 0.579019 | 4.4:+66781928 | None:intergenic |
| AAAGATGCGCAGAAAAGGGA+AGG | 0.579501 | 4.4:+66782339 | MS.gene88299:CDS |
| CACCAAGAGCCTGATAAGAT+CGG | 0.582662 | 4.4:-66782473 | None:intergenic |
| CCAGGTGTTCTGATGTTATG+GGG | 0.595199 | 4.4:+66782309 | MS.gene88299:CDS |
| AAGAGCGATGGGAAAGCCTG+TGG | 0.598960 | 4.4:+66781940 | MS.gene88299:CDS |
| AGGCACTCGGTGGACACAAG+AGG | 0.606213 | 4.4:+66782592 | MS.gene88299:CDS |
| ACAGAATCACAAATCAATAC+GGG | 0.613334 | 4.4:-66782656 | None:intergenic |
| CAACTATGGAGAAGAGCGAT+GGG | 0.616240 | 4.4:+66781929 | None:intergenic |
| TACTGCAAAAGAGACAATGA+TGG | 0.621835 | 4.4:+66782507 | MS.gene88299:CDS |
| AAGCCAGGTTCAACTGTGAG+AGG | 0.622627 | 4.4:+66782436 | MS.gene88299:CDS |
| ATCACAAATCAATACGGGTG+TGG | 0.631203 | 4.4:-66782651 | None:intergenic |
| GGCACTCGGTGGACACAAGA+GGG | 0.632635 | 4.4:+66782593 | MS.gene88299:CDS |
| CCACTTCCCACAAATCCCAC+AGG | 0.647858 | 4.4:-66781956 | None:intergenic |
| CGGCCGAGAAGTTAATGCTG+CGG | 0.653910 | 4.4:+66782260 | MS.gene88299:CDS |
| AGAGCGATGGGAAAGCCTGT+GGG | 0.654994 | 4.4:+66781941 | MS.gene88299:CDS |
| AGGTTCAACTGTGAGAGGTG+CGG | 0.663022 | 4.4:+66782441 | MS.gene88299:CDS |
| AAGATGCGCAGAAAAGGGAA+GGG | 0.667779 | 4.4:+66782340 | MS.gene88299:CDS |
| TTCGGGAAGGCAAGTCTGTG+AGG | 0.669270 | 4.4:-66782115 | None:intergenic |
| AAGTCTGCTCAGGCACTCGG+TGG | 0.670340 | 4.4:+66782582 | MS.gene88299:CDS |
| AGGCCACATGAGATCTCACA+TGG | 0.682156 | 4.4:+66782002 | MS.gene88299:CDS |
| ACCCGCAAGAGATCCAATTG+CGG | 0.686880 | 4.4:+66782240 | MS.gene88299:CDS |
| GAACCATGTGAGATCTCATG+TGG | 0.730676 | 4.4:-66782005 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | GATCAAGAAACAAAAAATGA+GGG | + | chr4.4:66782362-66782381 | MS.gene88299:CDS | 25.0% |
| !!! | ATTCTGTGTTTGTTGTATTT+CGG | - | chr4.4:66782136-66782155 | None:intergenic | 25.0% |
| AAAAATGTTCCGATCTTATC+AGG | + | chr4.4:66782464-66782483 | MS.gene88299:CDS | 30.0% | |
| GGATCAAGAAACAAAAAATG+AGG | + | chr4.4:66782361-66782380 | MS.gene88299:CDS | 30.0% | |
| ! | TGAGTATTAGTTTCAAGCTT+TGG | - | chr4.4:66782045-66782064 | None:intergenic | 30.0% |
| !! | TTCTGTGTTTGTTGTATTTC+GGG | - | chr4.4:66782135-66782154 | None:intergenic | 30.0% |
| !!! | TGAAGTTTTGTTTCAAGGAT+AGG | - | chr4.4:66782161-66782180 | None:intergenic | 30.0% |
| CAAAAAATGAGGGAAAACGA+TGG | + | chr4.4:66782372-66782391 | MS.gene88299:CDS | 35.0% | |
| TACTCAAGCTGTAGACAATT+CGG | + | chr4.4:66782059-66782078 | MS.gene88299:CDS | 35.0% | |
| TACTGCAAAAGAGACAATGA+TGG | + | chr4.4:66782507-66782526 | MS.gene88299:CDS | 35.0% | |
| TTACAAAGATGCGCAGAAAA+GGG | + | chr4.4:66782335-66782354 | MS.gene88299:CDS | 35.0% | |
| TTGCAATTTGAGCAGTGAAA+AGG | - | chr4.4:66782552-66782571 | None:intergenic | 35.0% | |
| TTTACAAAGATGCGCAGAAA+AGG | + | chr4.4:66782334-66782353 | MS.gene88299:CDS | 35.0% | |
| ! | AAGCAAATTGATTTCGACGA+TGG | - | chr4.4:66782095-66782114 | None:intergenic | 35.0% |
| ! | AGCAAATTGATTTCGACGAT+GGG | - | chr4.4:66782094-66782113 | None:intergenic | 35.0% |
| !! | ACTTTGAAATTCCTTAGCAC+TGG | - | chr4.4:66782628-66782647 | None:intergenic | 35.0% |
| ATCCAGGTGTTCTGATGTTA+TGG | + | chr4.4:66782307-66782326 | MS.gene88299:CDS | 40.0% | |
| GAAGATATGTCAAGAGATCC+AGG | + | chr4.4:66782291-66782310 | MS.gene88299:CDS | 40.0% | |
| TCCAGGTGTTCTGATGTTAT+GGG | + | chr4.4:66782308-66782327 | MS.gene88299:CDS | 40.0% | |
| !! | ATTTCGACGATGGGTTGAAT+CGG | - | chr4.4:66782085-66782104 | None:intergenic | 40.0% |
| !! | GTGTTTGTTGTATTTCGGGA+AGG | - | chr4.4:66782131-66782150 | None:intergenic | 40.0% |
| !! | GTTTCAAGCTTTGGAGCAAT+CGG | - | chr4.4:66782036-66782055 | None:intergenic | 40.0% |
| !!! | GGCAGTGAAGTTTTGTTTCA+AGG | - | chr4.4:66782166-66782185 | None:intergenic | 40.0% |
| AAAGATGCGCAGAAAAGGGA+AGG | + | chr4.4:66782339-66782358 | MS.gene88299:CDS | 45.0% | |
| AAGATGCGCAGAAAAGGGAA+GGG | + | chr4.4:66782340-66782359 | MS.gene88299:CDS | 45.0% | |
| AATTGGATCTCTTGCGGGTT+GGG | - | chr4.4:66782239-66782258 | None:intergenic | 45.0% | |
| ATTAACTTCTCGGCCGCAAT+TGG | - | chr4.4:66782256-66782275 | None:intergenic | 45.0% | |
| ATTTGTGGGAAGTGGTTCTC+CGG | + | chr4.4:66781964-66781983 | MS.gene88299:CDS | 45.0% | |
| CACCAAGAGCCTGATAAGAT+CGG | - | chr4.4:66782476-66782495 | None:intergenic | 45.0% | |
| CCAGGTGTTCTGATGTTATG+GGG | + | chr4.4:66782309-66782328 | MS.gene88299:CDS | 45.0% | |
| GAACCATGTGAGATCTCATG+TGG | - | chr4.4:66782008-66782027 | None:intergenic | 45.0% | |
| GATCTTATCAGGCTCTTGGT+GGG | + | chr4.4:66782475-66782494 | MS.gene88299:CDS | 45.0% | |
| TTCCGATCTTATCAGGCTCT+TGG | + | chr4.4:66782471-66782490 | MS.gene88299:CDS | 45.0% | |
| TTCTCTGTTTGAGCTTGCAG+AGG | - | chr4.4:66782190-66782209 | None:intergenic | 45.0% | |
| ! | CAAAGTGTTCAAGTCTGCTC+AGG | + | chr4.4:66782572-66782591 | MS.gene88299:CDS | 45.0% |
| AACGATGGCGAAAGCAGTAC+AGG | + | chr4.4:66782387-66782406 | MS.gene88299:CDS | 50.0% | |
| AAGCCAGGTTCAACTGTGAG+AGG | + | chr4.4:66782436-66782455 | MS.gene88299:CDS | 50.0% | |
| ACCCGCAAGAGATCCAATTG+CGG | + | chr4.4:66782240-66782259 | MS.gene88299:CDS | 50.0% | |
| AGATCTCATGTGGCCTCCAA+GGG | - | chr4.4:66781998-66782017 | None:intergenic | 50.0% | |
| AGGCCACATGAGATCTCACA+TGG | + | chr4.4:66782002-66782021 | MS.gene88299:CDS | 50.0% | |
| AGGTTCAACTGTGAGAGGTG+CGG | + | chr4.4:66782441-66782460 | MS.gene88299:CDS | 50.0% | |
| CAATTGGATCTCTTGCGGGT+TGG | - | chr4.4:66782240-66782259 | None:intergenic | 50.0% | |
| CAGCCGCAGCATTAACTTCT+CGG | - | chr4.4:66782266-66782285 | None:intergenic | 50.0% | |
| CCCCATAACATCAGAACACC+TGG | - | chr4.4:66782312-66782331 | None:intergenic | 50.0% | |
| CGATCTTATCAGGCTCTTGG+TGG | + | chr4.4:66782474-66782493 | MS.gene88299:CDS | 50.0% | |
| GGAAAGCCTGTGGGATTTGT+GGG | + | chr4.4:66781950-66781969 | MS.gene88299:CDS | 50.0% | |
| GGTACACTCTTCCAGTGCTA+AGG | + | chr4.4:66782614-66782633 | MS.gene88299:CDS | 50.0% | |
| TCTGTTTGAGCTTGCAGAGG+AGG | - | chr4.4:66782187-66782206 | None:intergenic | 50.0% | |
| TTCAAGTCTGCTCAGGCACT+CGG | + | chr4.4:66782579-66782598 | MS.gene88299:CDS | 50.0% | |
| !!! | CTCTTGCGGGTTGGGTTTTT+CGG | - | chr4.4:66782231-66782250 | None:intergenic | 50.0% |
| !!! | TCTTGCGGGTTGGGTTTTTC+GGG | - | chr4.4:66782230-66782249 | None:intergenic | 50.0% |
| AAGAGCGATGGGAAAGCCTG+TGG | + | chr4.4:66781940-66781959 | MS.gene88299:CDS | 55.0% | |
| AGAGCGATGGGAAAGCCTGT+GGG | + | chr4.4:66781941-66781960 | MS.gene88299:CDS | 55.0% | |
| CCACTTCCCACAAATCCCAC+AGG | - | chr4.4:66781959-66781978 | None:intergenic | 55.0% | |
| CCTGTGGGATTTGTGGGAAG+TGG | + | chr4.4:66781956-66781975 | MS.gene88299:CDS | 55.0% | |
| CGGCCGAGAAGTTAATGCTG+CGG | + | chr4.4:66782260-66782279 | MS.gene88299:CDS | 55.0% | |
| GAGATCTCATGTGGCCTCCA+AGG | - | chr4.4:66781999-66782018 | None:intergenic | 55.0% | |
| GCACCTCTCACAGTTGAACC+TGG | - | chr4.4:66782442-66782461 | None:intergenic | 55.0% | |
| GCCGCAATTGGATCTCTTGC+GGG | - | chr4.4:66782244-66782263 | None:intergenic | 55.0% | |
| GGCCGCAATTGGATCTCTTG+CGG | - | chr4.4:66782245-66782264 | None:intergenic | 55.0% | |
| GGGAAAGCCTGTGGGATTTG+TGG | + | chr4.4:66781949-66781968 | MS.gene88299:CDS | 55.0% | |
| TTCGGGAAGGCAAGTCTGTG+AGG | - | chr4.4:66782118-66782137 | None:intergenic | 55.0% | |
| !! | CACAAGCAGACTCTGAAGCC+AGG | + | chr4.4:66782421-66782440 | MS.gene88299:CDS | 55.0% |
| AAGTCTGCTCAGGCACTCGG+TGG | + | chr4.4:66782582-66782601 | MS.gene88299:CDS | 60.0% | |
| GTGGGAAGTGGTTCTCCGGT+GGG | + | chr4.4:66781968-66781987 | MS.gene88299:CDS | 60.0% | |
| TGTGGGAAGTGGTTCTCCGG+TGG | + | chr4.4:66781967-66781986 | MS.gene88299:CDS | 60.0% | |
| TTCTCCGGTGGGAAAGCCCT+TGG | + | chr4.4:66781979-66781998 | MS.gene88299:CDS | 60.0% | |
| ! | AGGCACTCGGTGGACACAAG+AGG | + | chr4.4:66782592-66782611 | MS.gene88299:CDS | 60.0% |
| ! | GGCACTCGGTGGACACAAGA+GGG | + | chr4.4:66782593-66782612 | MS.gene88299:CDS | 60.0% |
| !!! | GCGGGTTGGGTTTTTCGGGT+TGG | - | chr4.4:66782226-66782245 | None:intergenic | 60.0% |
| GCCTCCAAGGGCTTTCCCAC+CGG | - | chr4.4:66781986-66782005 | None:intergenic | 65.0% | |
| TCCGGTGGGAAAGCCCTTGG+AGG | + | chr4.4:66781982-66782001 | MS.gene88299:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 66781934 | 66782665 | 66781934 | ID=MS.gene88299 |
| chr4.4 | mRNA | 66781934 | 66782665 | 66781934 | ID=MS.gene88299.t1;Parent=MS.gene88299 |
| chr4.4 | exon | 66781934 | 66782665 | 66781934 | ID=MS.gene88299.t1.exon1;Parent=MS.gene88299.t1 |
| chr4.4 | CDS | 66781934 | 66782665 | 66781934 | ID=cds.MS.gene88299.t1;Parent=MS.gene88299.t1 |
| Gene Sequence |
| Protein sequence |
