AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene88392

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene88392.t1 MTR_3g025840 90.476 147 11 2 18 162 1 146 2.88e-89 268
MS.gene88392.t1 MTR_4g098870 94.231 104 6 0 166 269 256 359 1.65e-60 202
MS.gene88392.t1 MTR_4g098870 94.231 104 6 0 166 269 256 359 1.98e-60 201
MS.gene88392.t1 MTR_4g098870 94.231 104 6 0 166 269 276 379 2.43e-60 202
MS.gene88392.t1 MTR_4g098870 94.231 104 6 0 166 269 276 379 2.69e-60 202
MS.gene88392.t1 MTR_4g098870 94.231 104 6 0 166 269 295 398 3.11e-60 202
MS.gene88392.t1 MTR_5g014040 64.151 106 37 1 167 272 219 323 2.19e-32 125
MS.gene88392.t1 MTR_5g014040 64.151 106 37 1 167 272 259 363 3.10e-32 125
MS.gene88392.t1 MTR_5g014040 66.019 103 34 1 167 269 259 360 1.70e-31 123
MS.gene88392.t1 MTR_4g078810 66.038 53 18 0 217 269 155 207 2.07e-19 88.2
MS.gene88392.t1 MTR_2g090675 46.316 95 40 1 175 269 127 210 2.85e-19 87.8
MS.gene88392.t1 MTR_2g450070 62.963 54 20 0 216 269 209 262 1.14e-15 77.8
MS.gene88392.t1 MTR_2g450070 62.963 54 20 0 216 269 209 262 1.16e-15 77.8
MS.gene88392.t1 MTR_3g106220 55.224 67 29 1 213 278 191 257 8.30e-15 75.1
MS.gene88392.t1 MTR_4g131570 61.818 55 21 0 215 269 198 252 8.68e-15 75.1
MS.gene88392.t1 MTR_4g121020 54.930 71 29 2 219 286 110 180 1.23e-14 74.3
MS.gene88392.t1 MTR_4g121020 54.930 71 29 2 219 286 214 284 1.37e-14 74.3
MS.gene88392.t1 MTR_4g121020 54.930 71 29 2 219 286 214 284 1.41e-14 74.3
MS.gene88392.t1 MTR_4g131600 59.322 59 21 1 211 269 195 250 3.45e-14 73.2
MS.gene88392.t1 MTR_1g032570 57.377 61 25 1 219 278 130 190 4.02e-14 72.8
MS.gene88392.t1 MTR_1g032570 57.377 61 25 1 219 278 199 259 4.72e-14 72.8
MS.gene88392.t1 MTR_7g089010 60.784 51 20 0 219 269 90 140 1.56e-13 70.1
MS.gene88392.t1 MTR_4g131580 62.963 54 20 0 216 269 196 249 1.60e-13 71.2
MS.gene88392.t1 MTR_4g064730 60.784 51 20 0 219 269 130 180 2.54e-13 69.7
MS.gene88392.t1 MTR_7g117705 54.839 62 27 1 218 278 189 250 9.40e-13 68.6
MS.gene88392.t1 MTR_1g013170 53.226 62 28 1 218 278 189 250 1.06e-12 68.6
MS.gene88392.t1 MTR_3g102590 54.237 59 26 1 213 271 137 194 1.59e-12 65.5
MS.gene88392.t1 MTR_3g102590 50.725 69 30 2 213 278 137 204 3.13e-12 65.5
MS.gene88392.t1 MTR_2g034960 45.946 74 34 2 219 286 210 283 3.46e-12 67.0
MS.gene88392.t1 MTR_8g079940 56.452 62 26 1 219 279 208 269 3.73e-12 67.0
MS.gene88392.t1 MTR_1g013180 57.692 52 22 0 218 269 340 391 4.66e-12 67.0
MS.gene88392.t1 MTR_7g026400 50.000 62 30 1 219 279 156 217 7.50e-12 66.2
MS.gene88392.t1 MTR_3g102600 55.556 45 20 0 225 269 88 132 7.53e-12 62.4
MS.gene88392.t1 MTR_3g086100 50.000 66 32 1 216 280 124 189 1.00e-11 65.5
MS.gene88392.t1 MTR_3g086100 50.000 66 32 1 216 280 195 260 1.09e-11 65.5
MS.gene88392.t1 MTR_3g102600 55.556 45 20 0 225 269 137 181 1.35e-11 62.8
MS.gene88392.t1 MTR_4g021760 43.284 67 31 1 219 285 168 227 1.60e-11 64.3
MS.gene88392.t1 MTR_3g102600 55.556 45 20 0 225 269 88 132 2.08e-11 62.0
MS.gene88392.t1 MTR_3g102600 55.556 45 20 0 225 269 137 181 3.08e-11 62.4
MS.gene88392.t1 MTR_4g021845 50.000 52 26 0 219 270 167 218 3.52e-11 62.4
MS.gene88392.t1 MTR_4g086835 42.857 63 36 0 207 269 261 323 6.88e-11 62.8
MS.gene88392.t1 MTR_4g021790 45.455 66 36 0 204 269 152 217 7.61e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene88392.t1 AT1G18640 54.545 154 64 4 18 167 1 152 1.83e-40 142
MS.gene88392.t1 AT1G18640 54.545 154 64 4 18 167 1 152 2.26e-40 142
MS.gene88392.t1 AT4G18020 49.565 115 52 3 155 269 237 345 2.48e-20 91.3
MS.gene88392.t1 AT4G18020 49.565 115 52 3 155 269 237 345 2.48e-20 91.3
MS.gene88392.t1 AT4G18020 49.565 115 52 3 155 269 237 345 2.48e-20 91.3
MS.gene88392.t1 AT4G18020 49.565 115 52 3 155 269 237 345 2.48e-20 91.3
MS.gene88392.t1 AT4G18020 49.565 115 52 3 155 269 237 345 2.48e-20 91.3
MS.gene88392.t1 AT4G18020 49.565 115 52 3 155 269 237 345 2.48e-20 91.3
MS.gene88392.t1 AT4G18020 49.565 115 52 3 155 269 237 345 2.76e-20 91.3
MS.gene88392.t1 AT4G18020 49.565 115 52 3 155 269 237 345 2.76e-20 91.3
MS.gene88392.t1 AT4G18020 49.565 115 52 3 155 269 237 345 2.76e-20 91.3
MS.gene88392.t1 AT4G18020 49.565 115 52 3 155 269 237 345 2.76e-20 91.3
MS.gene88392.t1 AT4G18020 49.565 115 52 3 155 269 237 345 2.76e-20 91.3
MS.gene88392.t1 AT5G44190 75.000 52 13 0 218 269 145 196 7.09e-20 89.4
MS.gene88392.t1 AT5G44190 75.000 52 13 0 218 269 145 196 7.15e-20 89.0
MS.gene88392.t1 AT2G20570 73.077 52 14 0 218 269 151 202 2.50e-19 87.8
MS.gene88392.t1 AT2G20570 57.143 77 33 0 193 269 142 218 1.54e-18 85.9
MS.gene88392.t1 AT5G58080 64.815 54 19 0 216 269 190 243 1.58e-16 80.1
MS.gene88392.t1 AT2G01760 59.375 64 25 1 216 278 131 194 9.03e-16 76.6
MS.gene88392.t1 AT2G01760 59.375 64 25 1 216 278 196 259 1.90e-15 76.3
MS.gene88392.t1 AT2G01760 59.375 64 25 1 216 278 224 287 2.45e-15 76.3
MS.gene88392.t1 AT2G40970 46.835 79 42 0 191 269 76 154 7.81e-15 73.2
MS.gene88392.t1 AT3G10760 43.956 91 50 1 185 274 70 160 8.09e-15 74.3
MS.gene88392.t1 AT2G25180 61.290 62 23 1 218 278 193 254 1.44e-14 74.3
MS.gene88392.t1 AT3G16857 56.923 65 27 1 211 274 158 222 2.78e-14 73.6
MS.gene88392.t1 AT3G16857 56.923 65 27 1 211 274 228 292 3.67e-14 73.2
MS.gene88392.t1 AT3G16857 56.923 65 27 1 211 274 228 292 3.76e-14 73.2
MS.gene88392.t1 AT5G05090 60.784 51 20 0 219 269 80 130 5.27e-14 71.2
MS.gene88392.t1 AT5G59570 60.784 51 20 0 219 269 140 190 5.72e-14 71.2
MS.gene88392.t1 AT5G59570 60.784 51 20 0 219 269 140 190 5.72e-14 71.2
MS.gene88392.t1 AT3G46640 57.895 57 23 1 219 274 143 199 1.06e-13 70.9
MS.gene88392.t1 AT3G46640 57.895 57 23 1 219 274 143 199 1.06e-13 70.9
MS.gene88392.t1 AT3G46640 60.784 51 20 0 219 269 143 193 1.39e-13 70.5
MS.gene88392.t1 AT4G31920 45.570 79 42 1 218 295 181 259 1.75e-13 71.2
MS.gene88392.t1 AT5G49240 54.386 57 24 1 219 273 222 278 3.78e-12 65.9
MS.gene88392.t1 AT1G25550 50.847 59 27 2 216 273 206 263 1.85e-11 63.9
MS.gene88392.t1 AT3G62670 36.154 130 70 3 145 269 144 265 3.08e-11 63.9

Find 48 sgRNAs with CRISPR-Local

Find 164 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGCTATTCCTTCTCGAATAT+TGG 0.160903 8.3:-56783718 MS.gene88392:CDS
TTGGTGTTGGTTAGAAATTC+TGG 0.228056 8.3:-56785604 MS.gene88392:intron
ATCAGAACCAAATCTGAAAA+AGG 0.233092 8.3:-56786271 MS.gene88392:CDS
GGTATGTTTGGATTTCAAAT+TGG 0.293642 8.3:-56786249 MS.gene88392:CDS
GTAATGACATTGACACGAAT+TGG 0.304631 8.3:+56786321 None:intergenic
ATGGAAGCTTTGATCAGTTA+TGG 0.352182 8.3:-56786351 MS.gene88392:CDS
TAATGTGGCAAGCCATCTTC+AGG 0.386166 8.3:-56783664 MS.gene88392:CDS
TGGAAGCTTTGATCAGTTAT+GGG 0.406259 8.3:-56786350 MS.gene88392:CDS
TTGTTTAGAACCTTCACATT+TGG 0.411730 8.3:+56784049 None:intergenic
GCAGCCAATGCTTCCTCAAA+AGG 0.433886 8.3:+56785333 None:intergenic
TAATGATAAATTGATATTCA+AGG 0.434212 8.3:-56783580 MS.gene88392:CDS
GAATTCTATAAACCTTTAGA+AGG 0.443523 8.3:+56786138 None:intergenic
TTGAGGAAGCATTGGCTGCT+AGG 0.443908 8.3:-56785329 MS.gene88392:CDS
GAGAATACGGTTCCTTCTAA+AGG 0.454676 8.3:-56786150 MS.gene88392:intron
AATGTGAGATAAAAGCTAAT+CGG 0.462021 8.3:-56783976 MS.gene88392:CDS
ATTATGGCTGCTGCTACTGT+TGG 0.489665 8.3:-56786195 MS.gene88392:CDS
TAATGACATTGACACGAATT+GGG 0.491842 8.3:+56786322 None:intergenic
AATGTTGGATCCACGATCCA+TGG 0.499244 8.3:+56786393 None:intergenic
GGTTAGAAATTCTGGAGCTA+TGG 0.501559 8.3:-56785596 MS.gene88392:intron
TACTTTGGTCTCAGGTAGAC+TGG 0.503714 8.3:-56783797 MS.gene88392:intron
AGATTTGGTTCTGATTCAAA+CGG 0.513286 8.3:+56786279 None:intergenic
ATTCTGGAGCTATGGAAGAA+TGG 0.517491 8.3:-56785588 MS.gene88392:CDS
AATATTGGATCTAATGAAAG+TGG 0.519493 8.3:-56783703 MS.gene88392:CDS
GGTTAGAGATGGAGATTCCA+TGG 0.524678 8.3:-56786410 None:intergenic
ACCATATCCTTTCAGAAGGA+AGG 0.524944 8.3:-56784124 MS.gene88392:intron
ACAACATCTGCAAATAAAGT+AGG 0.528162 8.3:-56784013 MS.gene88392:CDS
TGCTGCTATGCACTTCATCA+TGG 0.528512 8.3:+56785124 None:intergenic
ATTAGATCCAATATTCGAGA+AGG 0.541398 8.3:+56783711 None:intergenic
CCATATCCTTTCAGAAGGAA+GGG 0.544483 8.3:-56784123 MS.gene88392:intron
GTGAAGGCAGTGGAGAAACT+AGG 0.549880 8.3:-56783750 MS.gene88392:CDS
GTAGCAGCAGCCATAATCAA+AGG 0.552287 8.3:+56786201 None:intergenic
ACTGTTGGAAATTCACAAGT+TGG 0.556078 8.3:-56786180 MS.gene88392:CDS
GGTAGATTATAACCTGAAGA+TGG 0.564115 8.3:+56783652 None:intergenic
CTGTTGCAGAGTGGACTGCT+AGG 0.572221 8.3:-56785479 MS.gene88392:intron
ACCACAGTTACCAAATGTGA+AGG 0.574268 8.3:-56784059 MS.gene88392:CDS
GGTTATAATCTACCTTCCCA+CGG 0.590624 8.3:-56783643 MS.gene88392:CDS
AGCACTGTGTGCATTGATGA+AGG 0.592504 8.3:-56785540 MS.gene88392:CDS
ACCTTCACATTTGGTAACTG+TGG 0.592713 8.3:+56784058 None:intergenic
ACTCTCACACTTAGAAGTGT+CGG 0.606788 8.3:+56784090 None:intergenic
CATATCCTTTCAGAAGGAAG+GGG 0.608637 8.3:-56784122 MS.gene88392:intron
ATTGGATCTAATGAAAGTGG+AGG 0.613074 8.3:-56783700 MS.gene88392:CDS
ATCCTTTCAGAAGGAAGGGG+AGG 0.613484 8.3:-56784119 MS.gene88392:intron
TAATGCAGTGTGCTTTGATG+TGG 0.618387 8.3:-56785565 MS.gene88392:CDS
GGGCTTGACAAGGCATAATG+TGG 0.631155 8.3:-56783679 MS.gene88392:CDS
TTGGATCTAATGAAAGTGGA+GGG 0.637148 8.3:-56783699 MS.gene88392:CDS
GATGGAGATTCCATGGATCG+TGG 0.638206 8.3:-56786403 MS.gene88392:CDS
TGAAAGTGGAGGGCTTGACA+AGG 0.644049 8.3:-56783689 MS.gene88392:CDS
CAGGAAAGGCTGTTGCAGAG+TGG 0.649400 8.3:-56785488 MS.gene88392:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTATATTTTTGAATTTCTT+TGG - chr8.3:56784547-56784566 MS.gene88392:intron 10.0%
!! TAATGATAAATTGATATTCA+AGG - chr8.3:56786385-56786404 MS.gene88392:CDS 15.0%
!!! ATTAATTACAATTTTGAATG+AGG + chr8.3:56786369-56786388 None:intergenic 15.0%
!!! ATTTTAAAATACTAAGCTTT+AGG + chr8.3:56785165-56785184 None:intergenic 15.0%
!!! GTAATTGGTAATAAATTAAA+GGG + chr8.3:56784914-56784933 None:intergenic 15.0%
!! AAAACTGTATTAAGAAAATC+AGG + chr8.3:56786146-56786165 None:intergenic 20.0%
!! AAATATCAGTAATATTAGAC+AGG + chr8.3:56785317-56785336 None:intergenic 20.0%
!! TGTAATTTAATCATATGTGT+TGG - chr8.3:56784002-56784021 MS.gene88392:CDS 20.0%
!!! ATGTATGTTTGCAATTTTTT+AGG - chr8.3:56785624-56785643 MS.gene88392:intron 20.0%
!!! GGAAATTCTTTTTTGATAAA+GGG - chr8.3:56784524-56784543 MS.gene88392:intron 20.0%
!!! GGTAATTGGTAATAAATTAA+AGG + chr8.3:56784915-56784934 None:intergenic 20.0%
!!! TACTAGTTTACTTGATTTTT+AGG - chr8.3:56785112-56785131 MS.gene88392:intron 20.0%
!!! TGATTTTCTCAAATTATTAG+CGG + chr8.3:56783972-56783991 None:intergenic 20.0%
!!! TGATTTTCTTAATACAGTTT+TGG - chr8.3:56786145-56786164 MS.gene88392:intron 20.0%
!!! TGGAAATTCTTTTTTGATAA+AGG - chr8.3:56784523-56784542 MS.gene88392:intron 20.0%
! AAATAACGATATACGGAAAT+TGG + chr8.3:56785797-56785816 None:intergenic 25.0%
! AACATTATGAGAAGAATGAA+TGG + chr8.3:56786040-56786059 None:intergenic 25.0%
! AAGCATGAAATAACGATATA+CGG + chr8.3:56785804-56785823 None:intergenic 25.0%
! AATATTGGATCTAATGAAAG+TGG - chr8.3:56786262-56786281 MS.gene88392:CDS 25.0%
! AATGTGAGATAAAAGCTAAT+CGG - chr8.3:56785989-56786008 MS.gene88392:intron 25.0%
! AGATTAAGCATGTAATCAAA+AGG - chr8.3:56785482-56785501 MS.gene88392:CDS 25.0%
! ATAGAAAAACACTAGGTAAT+TGG + chr8.3:56784929-56784948 None:intergenic 25.0%
! CAAAGATACAAATTTAACAC+AGG + chr8.3:56785375-56785394 None:intergenic 25.0%
! GAATTCTATAAACCTTTAGA+AGG + chr8.3:56783830-56783849 None:intergenic 25.0%
! GTAATAAATTAAAGGGATGA+GGG + chr8.3:56784907-56784926 None:intergenic 25.0%
! TAATAAATTAAAGGGATGAG+GGG + chr8.3:56784906-56784925 None:intergenic 25.0%
! TCAAAAATTTCACAGTAGAA+TGG + chr8.3:56785457-56785476 None:intergenic 25.0%
!! TATATCGTTATTTCATGCTT+TGG - chr8.3:56785804-56785823 MS.gene88392:intron 25.0%
!!! TGAGGTATTGAAATAGTTTT+TGG + chr8.3:56786351-56786370 None:intergenic 25.0%
!!! TTAATACAGTTTTGGTACTT+TGG - chr8.3:56786153-56786172 MS.gene88392:CDS 25.0%
!!! TTGATTTTCATTTTTCACCA+TGG - chr8.3:56784096-56784115 MS.gene88392:CDS 25.0%
AAATTGATGAATGGTGTAGA+GGG - chr8.3:56784691-56784710 MS.gene88392:intron 30.0%
AATAAATTAAAGGGATGAGG+GGG + chr8.3:56784905-56784924 None:intergenic 30.0%
ACAACATCTGCAAATAAAGT+AGG - chr8.3:56785952-56785971 MS.gene88392:intron 30.0%
AGTGAGAAGACAAAAAATGT+TGG + chr8.3:56783590-56783609 None:intergenic 30.0%
ATAGGTGTACGCTATATTAT+TGG + chr8.3:56785032-56785051 None:intergenic 30.0%
ATCAGAACCAAATCTGAAAA+AGG - chr8.3:56783694-56783713 MS.gene88392:CDS 30.0%
ATTAGATCCAATATTCGAGA+AGG + chr8.3:56786257-56786276 None:intergenic 30.0%
CAATGAACAGTGTGAAATAA+CGG + chr8.3:56786069-56786088 None:intergenic 30.0%
CTTGAATTGAAAGAGTCAAT+AGG + chr8.3:56785050-56785069 None:intergenic 30.0%
GAATATAACCTTATGCAACA+TGG + chr8.3:56786097-56786116 None:intergenic 30.0%
GGAAACTTGAAATTGATGAA+TGG - chr8.3:56784682-56784701 MS.gene88392:intron 30.0%
GGTAATAAATTAAAGGGATG+AGG + chr8.3:56784908-56784927 None:intergenic 30.0%
GGTATGTTTGGATTTCAAAT+TGG - chr8.3:56783716-56783735 MS.gene88392:CDS 30.0%
GTTCATGATAGAAAAACACT+AGG + chr8.3:56784936-56784955 None:intergenic 30.0%
TAATGACATTGACACGAATT+GGG + chr8.3:56783646-56783665 None:intergenic 30.0%
TCAGAACCAAATCTGAAAAA+GGG - chr8.3:56783695-56783714 MS.gene88392:CDS 30.0%
TTGTTTATCCATGTTGCATA+AGG - chr8.3:56786086-56786105 MS.gene88392:intron 30.0%
! AATCTGAAAAAGGGTATGTT+TGG - chr8.3:56783704-56783723 MS.gene88392:CDS 30.0%
! CTGATATTTCTGCTATATCA+TGG - chr8.3:56785328-56785347 MS.gene88392:CDS 30.0%
! GAAGTTTTCTCCTTTGATTA+TGG - chr8.3:56783754-56783773 MS.gene88392:CDS 30.0%
! TTAATCATATGTGTTGGTGT+TGG - chr8.3:56784008-56784027 MS.gene88392:CDS 30.0%
! TTGAATTTTCACAGAGCAAT+GGG - chr8.3:56784599-56784618 MS.gene88392:intron 30.0%
! TTGTTTAGAACCTTCACATT+TGG + chr8.3:56785919-56785938 None:intergenic 30.0%
!! AAATAGTTTTTGGAAACCGT+GGG + chr8.3:56786341-56786360 None:intergenic 30.0%
!! AGATTTGGTTCTGATTCAAA+CGG + chr8.3:56783689-56783708 None:intergenic 30.0%
!! AGTTGGTCATTTTGAGAATA+CGG - chr8.3:56783802-56783821 MS.gene88392:intron 30.0%
!! ATTTTCATTTTTCACCATGG+TGG - chr8.3:56784099-56784118 MS.gene88392:CDS 30.0%
!! CAATTTCAAGTTTCCATAGA+GGG + chr8.3:56784677-56784696 None:intergenic 30.0%
!! CTATTGACTCTTTCAATTCA+AGG - chr8.3:56785048-56785067 MS.gene88392:intron 30.0%
!! TCAATTTCAAGTTTCCATAG+AGG + chr8.3:56784678-56784697 None:intergenic 30.0%
!! TTTTCATTTTTCACCATGGT+GGG - chr8.3:56784100-56784119 MS.gene88392:CDS 30.0%
!!! AACATACCCTTTTTCAGATT+TGG + chr8.3:56783704-56783723 None:intergenic 30.0%
AAAATCTTACTGCTATGCAG+TGG + chr8.3:56785752-56785771 None:intergenic 35.0%
AATTGATGAATGGTGTAGAG+GGG - chr8.3:56784692-56784711 MS.gene88392:intron 35.0%
ACTGTTGGAAATTCACAAGT+TGG - chr8.3:56783785-56783804 MS.gene88392:CDS 35.0%
ATCTTTGTTCAATCCCTCTA+TGG - chr8.3:56784661-56784680 MS.gene88392:intron 35.0%
ATGAAGAACTAGATGTGTCT+CGG + chr8.3:56784037-56784056 None:intergenic 35.0%
ATTGGATCTAATGAAAGTGG+AGG - chr8.3:56786265-56786284 MS.gene88392:CDS 35.0%
CAAAAAATTTGTGAAGGCAG+TGG - chr8.3:56786205-56786224 MS.gene88392:CDS 35.0%
CATGAACCTCCTAGAAAATA+AGG - chr8.3:56784949-56784968 MS.gene88392:intron 35.0%
GAAATTGATGAATGGTGTAG+AGG - chr8.3:56784690-56784709 MS.gene88392:intron 35.0%
GCTAGGTTAGATTGTTCAAA+TGG - chr8.3:56784503-56784522 MS.gene88392:intron 35.0%
GCTGCACAAAAAATTTGTGA+AGG - chr8.3:56786199-56786218 MS.gene88392:CDS 35.0%
GGAACATGACATGGAAAATA+TGG - chr8.3:56784714-56784733 MS.gene88392:intron 35.0%
GGTAGATTATAACCTGAAGA+TGG + chr8.3:56786316-56786335 None:intergenic 35.0%
GTAATGACATTGACACGAAT+TGG + chr8.3:56783647-56783666 None:intergenic 35.0%
GTCACTGCATCAAAAATTGA+AGG + chr8.3:56784798-56784817 None:intergenic 35.0%
TCAAAAATTGAAGGAGTCAC+CGG + chr8.3:56784789-56784808 None:intergenic 35.0%
TTGGATCTAATGAAAGTGGA+GGG - chr8.3:56786266-56786285 MS.gene88392:CDS 35.0%
! ACTGCATAGCAGTAAGATTT+TGG - chr8.3:56785751-56785770 MS.gene88392:intron 35.0%
! ATGGAAGCTTTGATCAGTTA+TGG - chr8.3:56783614-56783633 MS.gene88392:CDS 35.0%
! GTTGAATTTTCACAGAGCAA+TGG - chr8.3:56784598-56784617 MS.gene88392:intron 35.0%
! TGGAAGCTTTGATCAGTTAT+GGG - chr8.3:56783615-56783634 MS.gene88392:CDS 35.0%
! TTTTGTCTTCTCACTGTACA+TGG - chr8.3:56783595-56783614 MS.gene88392:CDS 35.0%
!! GAAATAGTTTTTGGAAACCG+TGG + chr8.3:56786342-56786361 None:intergenic 35.0%
!! TCCATGTTAAGTTGGAATGA+CGG - chr8.3:56784329-56784348 MS.gene88392:intron 35.0%
!! TGTTGACTTCCATGTTAAGT+TGG - chr8.3:56784321-56784340 MS.gene88392:intron 35.0%
!! TTGGTGTTGGTTAGAAATTC+TGG - chr8.3:56784361-56784380 MS.gene88392:intron 35.0%
!!! ACAAATTTTTTGTGCAGCTC+TGG + chr8.3:56786197-56786216 None:intergenic 35.0%
!!! TGCAAACCTTATTTTCTAGG+AGG + chr8.3:56784958-56784977 None:intergenic 35.0%
!!! TGGTGCAAACCTTATTTTCT+AGG + chr8.3:56784961-56784980 None:intergenic 35.0%
ACCACAGTTACCAAATGTGA+AGG - chr8.3:56785906-56785925 MS.gene88392:intron 40.0%
ACCATATCCTTTCAGAAGGA+AGG - chr8.3:56785841-56785860 MS.gene88392:intron 40.0%
ACCGTCATTCCAACTTAACA+TGG + chr8.3:56784333-56784352 None:intergenic 40.0%
AGTTTCAAAAACACCCACCA+TGG + chr8.3:56784116-56784135 None:intergenic 40.0%
ATGGACCATATCCTTTCAGA+AGG - chr8.3:56785837-56785856 MS.gene88392:intron 40.0%
ATTGATGAATGGTGTAGAGG+GGG - chr8.3:56784693-56784712 MS.gene88392:intron 40.0%
CATAACATGAAACTGCGTAG+AGG - chr8.3:56785592-56785611 MS.gene88392:CDS 40.0%
CATATCCTTTCAGAAGGAAG+GGG - chr8.3:56785843-56785862 MS.gene88392:intron 40.0%
CCATATCCTTTCAGAAGGAA+GGG - chr8.3:56785842-56785861 MS.gene88392:intron 40.0%
CGCTATTCCTTCTCGAATAT+TGG - chr8.3:56786247-56786266 MS.gene88392:CDS 40.0%
CTCTACGCAGTTTCATGTTA+TGG + chr8.3:56785594-56785613 None:intergenic 40.0%
GAGAATACGGTTCCTTCTAA+AGG - chr8.3:56783815-56783834 MS.gene88392:intron 40.0%
GGTTATAATCTACCTTCCCA+CGG - chr8.3:56786322-56786341 MS.gene88392:CDS 40.0%
TAATGCAGTGTGCTTTGATG+TGG - chr8.3:56784400-56784419 MS.gene88392:intron 40.0%
TCTGAAAGGATATGGTCCAT+CGG + chr8.3:56785837-56785856 None:intergenic 40.0%
TTACAGTCACAAGAGAGAGA+TGG + chr8.3:56784981-56785000 None:intergenic 40.0%
! AATTTTCACAGAGCAATGGG+TGG - chr8.3:56784602-56784621 MS.gene88392:intron 40.0%
! ACCTTCACATTTGGTAACTG+TGG + chr8.3:56785910-56785929 None:intergenic 40.0%
! ACTCTCACACTTAGAAGTGT+CGG + chr8.3:56785878-56785897 None:intergenic 40.0%
! ATGCTTTGGATTCTTTCCGA+TGG - chr8.3:56785818-56785837 MS.gene88392:intron 40.0%
!! AGTTTTGGTACTTTGGTCTC+AGG - chr8.3:56786160-56786179 MS.gene88392:CDS 40.0%
!! ATTCTGGAGCTATGGAAGAA+TGG - chr8.3:56784377-56784396 MS.gene88392:intron 40.0%
!! CTTGCTGCAAGTTTTGTAAC+CGG - chr8.3:56784767-56784786 MS.gene88392:intron 40.0%
!! GGTTAGAAATTCTGGAGCTA+TGG - chr8.3:56784369-56784388 MS.gene88392:intron 40.0%
!! TGTTCCTTTTGAGGAAGCAT+TGG - chr8.3:56784628-56784647 MS.gene88392:intron 40.0%
!!! ATGAGCTTGCTGAATTTTGC+GGG - chr8.3:56784453-56784472 MS.gene88392:intron 40.0%
!!! GATGAGCTTGCTGAATTTTG+CGG - chr8.3:56784452-56784471 MS.gene88392:intron 40.0%
AATGTTGGATCCACGATCCA+TGG + chr8.3:56783575-56783594 None:intergenic 45.0%
ATGATGAAGTGCATAGCAGC+AGG - chr8.3:56784843-56784862 MS.gene88392:intron 45.0%
ATTATGGCTGCTGCTACTGT+TGG - chr8.3:56783770-56783789 MS.gene88392:CDS 45.0%
GTAGCAGCAGCCATAATCAA+AGG + chr8.3:56783767-56783786 None:intergenic 45.0%
TAATGTGGCAAGCCATCTTC+AGG - chr8.3:56786301-56786320 MS.gene88392:CDS 45.0%
TACTTTGGTCTCAGGTAGAC+TGG - chr8.3:56786168-56786187 MS.gene88392:CDS 45.0%
TGATGAAGTGCATAGCAGCA+GGG - chr8.3:56784844-56784863 MS.gene88392:intron 45.0%
TGCTGCTATGCACTTCATCA+TGG + chr8.3:56784844-56784863 None:intergenic 45.0%
! AGCACTGTGTGCATTGATGA+AGG - chr8.3:56784425-56784444 MS.gene88392:intron 45.0%
! ATTTTGAACCCCCTAACCCA+AGG - chr8.3:56785715-56785734 MS.gene88392:intron 45.0%
! CCCTTCCTTCTGAAAGGATA+TGG + chr8.3:56785845-56785864 None:intergenic 45.0%
!! AGTTTTTGGAAACCGTGGGA+AGG + chr8.3:56786337-56786356 None:intergenic 45.0%
!!! ACGGTGTTGTGTTTTGGTGT+TGG - chr8.3:56784348-56784367 MS.gene88392:intron 45.0%
!!! GGAATGACGGTGTTGTGTTT+TGG - chr8.3:56784342-56784361 MS.gene88392:intron 45.0%
!!! TGAGCTTGCTGAATTTTGCG+GGG - chr8.3:56784454-56784473 MS.gene88392:intron 45.0%
!! AGAATTTAAAAAATAATATA+AGG + chr8.3:56785269-56785288 None:intergenic 5.0%
!! TAGTAAAAAAATAAAAATAT+AGG + chr8.3:56785238-56785257 None:intergenic 5.0%
ATCCTTTCAGAAGGAAGGGG+AGG - chr8.3:56785846-56785865 MS.gene88392:intron 50.0%
GATGAAGTGCATAGCAGCAG+GGG - chr8.3:56784845-56784864 MS.gene88392:intron 50.0%
GCAGCCAATGCTTCCTCAAA+AGG + chr8.3:56784635-56784654 None:intergenic 50.0%
GGGCTTGACAAGGCATAATG+TGG - chr8.3:56786286-56786305 MS.gene88392:CDS 50.0%
GTGAAGGCAGTGGAGAAACT+AGG - chr8.3:56786215-56786234 MS.gene88392:CDS 50.0%
TGAAAGTGGAGGGCTTGACA+AGG - chr8.3:56786276-56786295 MS.gene88392:CDS 50.0%
TGTAGAGGGGGAACATGACA+TGG - chr8.3:56784705-56784724 MS.gene88392:intron 50.0%
TTCCTCCCCTTCCTTCTGAA+AGG + chr8.3:56785851-56785870 None:intergenic 50.0%
! TGAATTTTGCGGGGCAGGAA+AGG - chr8.3:56784463-56784482 MS.gene88392:intron 50.0%
!! GGGTGGTTCTGTTCCTTTTG+AGG - chr8.3:56784619-56784638 MS.gene88392:intron 50.0%
!! TTGAGGAAGCATTGGCTGCT+AGG - chr8.3:56784636-56784655 MS.gene88392:intron 50.0%
ATGCACAGACACTGACACGC+GGG - chr8.3:56783944-56783963 MS.gene88392:intron 55.0%
CAGGAAAGGCTGTTGCAGAG+TGG - chr8.3:56784477-56784496 MS.gene88392:intron 55.0%
CGGAGCAAGCTTGTATCCAG+GGG - chr8.3:56784868-56784887 MS.gene88392:intron 55.0%
GATGCACAGACACTGACACG+CGG - chr8.3:56783943-56783962 MS.gene88392:intron 55.0%
GCGGAGCAAGCTTGTATCCA+GGG - chr8.3:56784867-56784886 MS.gene88392:intron 55.0%
GTTGCGAGGACCTTGGGTTA+GGG + chr8.3:56785728-56785747 None:intergenic 55.0%
! CTTGCTGAATTTTGCGGGGC+AGG - chr8.3:56784458-56784477 MS.gene88392:intron 55.0%
! TTGCGAGGACCTTGGGTTAG+GGG + chr8.3:56785727-56785746 None:intergenic 55.0%
!! CTGTTGCAGAGTGGACTGCT+AGG - chr8.3:56784486-56784505 MS.gene88392:intron 55.0%
AGTGGCGTTGCGAGGACCTT+GGG + chr8.3:56785734-56785753 None:intergenic 60.0%
CGTTGCGAGGACCTTGGGTT+AGG + chr8.3:56785729-56785748 None:intergenic 60.0%
GAAGTGCATAGCAGCAGGGG+CGG - chr8.3:56784848-56784867 MS.gene88392:intron 60.0%
GGCGGAGCAAGCTTGTATCC+AGG - chr8.3:56784866-56784885 MS.gene88392:intron 60.0%
TGCTATGCAGTGGCGTTGCG+AGG + chr8.3:56785742-56785761 None:intergenic 60.0%
! TGCGAGGACCTTGGGTTAGG+GGG + chr8.3:56785726-56785745 None:intergenic 60.0%
CAGTGGCGTTGCGAGGACCT+TGG + chr8.3:56785735-56785754 None:intergenic 65.0%
GGGGGTTCAGCTGCATCCCC+TGG + chr8.3:56784887-56784906 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 56783563 56786424 56783563 ID=MS.gene88392
chr8.3 mRNA 56783563 56786424 56783563 ID=MS.gene88392.t1;Parent=MS.gene88392
chr8.3 exon 56786151 56786424 56786151 ID=MS.gene88392.t1.exon1;Parent=MS.gene88392.t1
chr8.3 CDS 56786151 56786424 56786151 ID=cds.MS.gene88392.t1;Parent=MS.gene88392.t1
chr8.3 exon 56785480 56785612 56785480 ID=MS.gene88392.t1.exon2;Parent=MS.gene88392.t1
chr8.3 CDS 56785480 56785612 56785480 ID=cds.MS.gene88392.t1;Parent=MS.gene88392.t1
chr8.3 exon 56785321 56785374 56785321 ID=MS.gene88392.t1.exon3;Parent=MS.gene88392.t1
chr8.3 CDS 56785321 56785374 56785321 ID=cds.MS.gene88392.t1;Parent=MS.gene88392.t1
chr8.3 exon 56785137 56785173 56785137 ID=MS.gene88392.t1.exon4;Parent=MS.gene88392.t1
chr8.3 CDS 56785137 56785173 56785137 ID=cds.MS.gene88392.t1;Parent=MS.gene88392.t1
chr8.3 exon 56783964 56784131 56783964 ID=MS.gene88392.t1.exon5;Parent=MS.gene88392.t1
chr8.3 CDS 56783964 56784131 56783964 ID=cds.MS.gene88392.t1;Parent=MS.gene88392.t1
chr8.3 exon 56783563 56783805 56783563 ID=MS.gene88392.t1.exon6;Parent=MS.gene88392.t1
chr8.3 CDS 56783563 56783805 56783563 ID=cds.MS.gene88392.t1;Parent=MS.gene88392.t1
Gene Sequence

>MS.gene88392

ATGGAGATTCCATGGATCGTGGATCCAACATTTTTTGTCTTCTCACTGTACATGGAAGCTTTGATCAGTTATGGGATCAACCCAATTCGTGTCAATGTCATTACAAAGCAGCGATCTTGTTTTCCGTTTGAATCAGAACCAAATCTGAAAAAGGGTATGTTTGGATTTCAAATTGGAGTCGTGAACAAGAAGAAGTTTTCTCCTTTGATTATGGCTGCTGCTACTGTTGGAAATTCACAAGTTGGTCATTTTGAGAATACGGTTCCTTCTAAAGGTTTATAGAATTCTTACATTTTTGTATCTTTTTATCTATTTTATAATATAGTTCATAATTATATTTCATTATTTTGATCTAGTTATATTTAGTAGTTGATAACTATGATGCACAGACACTGACACGCGGGTGCCGCTAATAATTTGAGAAAATCATATAATTCAGTGTAATTTAATCATATGTGTTGGTGTTGGACACCGAGACACATCTAGTTCTTCATTGCTAATGTAATCTAGTTATATTTCATTGTTTTGATATATTGATTTTCATTTTTCACCATGGTGGGTGTTTTTGAAACTCTGACAAAATAGAGAAACATGATTGAATGTTTGTAATTCTTGCTGTTTGTACATAGTTATTAATTATTTTAAAATAGTGTGTTTTAAATATTCACTAATATTAAAGCACACTGATAATTGCAACAACAAAAAAAAAACATTATTTTAGCATTTTATGTGTTACTGTTAGTGTAAATTCTCAATGTTGTTGACTTCCATGTTAAGTTGGAATGACGGTGTTGTGTTTTGGTGTTGGTTAGAAATTCTGGAGCTATGGAAGAATGGTAATGCAGTGTGCTTTGATGTGGATAGCACTGTGTGCATTGATGAAGGAATTGATGAGCTTGCTGAATTTTGCGGGGCAGGAAAGGCTGTTGCAGAGTGGACTGCTAGGTTAGATTGTTCAAATGGAAATTCTTTTTTGATAAAGGGTTTATATTTTTGAATTTCTTTGGTATCTCTTTTGTTATATTTGATTTAAGTGTTGAATTTTCACAGAGCAATGGGTGGTTCTGTTCCTTTTGAGGAAGCATTGGCTGCTAGGTTATCTTTGTTCAATCCCTCTATGGAAACTTGAAATTGATGAATGGTGTAGAGGGGGAACATGACATGGAAAATATGGTGAAAATGTTGACATTGATGCTTTTTTGTGCTTGCTGCAAGTTTTGTAACCGGTGACTCCTTCAATTTTTGATGCAGTGACTTCAAACAGCGATTGTCTGAACACCATGATGAAGTGCATAGCAGCAGGGGCGGAGCAAGCTTGTATCCAGGGGATGCAGCTGAACCCCCTCATCCCTTTAATTTATTACCAATTACCTAGTGTTTTTCTATCATGAACCTCCTAGAAAATAAGGTTTGCACCATCTCTCTCTTGTGACTGTAAAAGTTGCACACATATTACAGCATGTTGTCCAATAATATAGCGTACACCTATTGACTCTTTCAATTCAAGGCTAACATTGATCTTTTCTATTGACTCTTTACTTTAACGTGTTACTAGTTTACTTGATTTTTAGGTGTTATTAGTTTACTACTTCTGCACATCCTAAAGCTTAGTATTTTAAAATGACTAGATCACTACATCAAACTAAGAACAAGATATATATTGTACAACATTCCTATATTTTTATTTTTTTACTATTTCTATTCCTTATATTATTTTTTAAATTCTTTTTTACTTTTTCTAGAGACTATTTCCTGTCTAATATTACTGATATTTCTGCTATATCATGGATGTGCATATTTCTGCTATTTCCTGTGTTAAATTTGTATCTTTGAAAAAATAAAATGATCTTTAAAAAAAGAACTGATTGAAACTAAAAGACGTATTGCTTATCCATTCTACTGTGAAATTTTTGAAAACTAGATTAAGCATGTAATCAAAAGGAAGATGAATTGACGAACGATTGTCTCGTGACATATCGAAAGAGATATATTCGACAACATTGATAAAGAGTTTATTACTCAACAAATCCATAACATGAAACTGCGTAGAGGTCAATTGTAATGTATGTTTGCAATTTTTTAGGTACAATGTAATTTTTTTGAATAAATATTATTTTGTATATATTATTATAATTATTAATTAAAGTTTTAAATTTTGAACCCCCTAACCCAAGGTCCTCGCAACGCCACTGCATAGCAGTAAGATTTTGGTTAAACTCTTTGAGCTAAATCCAATTTCCGTATATCGTTATTTCATGCTTTGGATTCTTTCCGATGGACCATATCCTTTCAGAAGGAAGGGGAGGAATTTGCCGACACTTCTAAGTGTGAGAGTGTTGTTTCACCACAGTTACCAAATGTGAAGGTTCTAAACAATAGTGAAAGTAATACAACATCTGCAAATAAAGTAGGTATTCGCAGTCATAAATGTGAGATAAAAGCTAATCGGAAGAAGATGAAAGTAAGCTATTTTTCCATTCATTCTTCTCATAATGTTCTTGTTCCGTTATTTCACACTGTTCATTGTTTATCCATGTTGCATAAGGTTATATTCATTTTATGTATGAGTGATTTACTGAACCTGATTTTCTTAATACAGTTTTGGTACTTTGGTCTCAGGTAGACTGGACGCCAGAGCTGCACAAAAAATTTGTGAAGGCAGTGGAGAAACTAGGCATTGATCACGCTATTCCTTCTCGAATATTGGATCTAATGAAAGTGGAGGGCTTGACAAGGCATAATGTGGCAAGCCATCTTCAGGTTATAATCTACCTTCCCACGGTTTCCAAAAACTATTTCAATACCTCATTCAAAATTGTAATTAATGATAAATTGATATTCAAGGTAGTTAAATATGCTTGA
Protein sequence

>MS.gene88392.t1

MEIPWIVDPTFFVFSLYMEALISYGINPIRVNVITKQRSCFPFESEPNLKKGMFGFQIGVVNKKKFSPLIMAAATVGNSQVGHFENTVPSKEILELWKNGNAVCFDVDSTVCIDEGIDELAEFCGAGKAVAEWTARAMGGSVPFEEALAARLSFDFKQRLSEHHDEKEGEEFADTSKCESVVSPQLPNVKVLNNSESNTTSANKVGIRSHKCEIKANRKKMKVDWTPELHKKFVKAVEKLGIDHAIPSRILDLMKVEGLTRHNVASHLQVIIYLPTVSKNYFNTSFKIVINDKLIFKVVKYA