Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88716.t1 | OMJ84645.1 | 42 | 88 | 49 | 1 | 44 | 131 | 50 | 135 | 7.10E-07 | 63.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88716.t1 | Q9FKI4 | 35.0 | 137 | 76 | 4 | 4 | 131 | 2 | 134 | 8.5e-09 | 61.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88716.t1 | A0A1R2C6T9 | 42.0 | 88 | 49 | 1 | 44 | 131 | 50 | 135 | 5.1e-07 | 63.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene88716.t1 | TR | GNAT |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88716.t1 | MTR_3g034200 | 34.532 | 139 | 74 | 4 | 4 | 131 | 2 | 134 | 2.05e-12 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88716.t1 | AT5G13780 | 42.045 | 88 | 47 | 2 | 44 | 131 | 51 | 134 | 1.02e-11 | 60.1 |
Find 32 sgRNAs with CRISPR-Local
Find 41 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTATCTTGCCTTTGAGTTC+TGG | 0.203816 | 5.4:+54193380 | MS.gene88716:CDS |
| TTGTATAAGCCGATGCCTTC+TGG | 0.236300 | 5.4:+54193915 | MS.gene88716:CDS |
| AAACCTGTTCCAAATCCTTA+TGG | 0.275073 | 5.4:-54193586 | None:intergenic |
| TATCGTAATCCAAGGCCTTT+TGG | 0.277027 | 5.4:-54193857 | None:intergenic |
| GACTGCCGCCCATAAGGATT+TGG | 0.362443 | 5.4:+54193577 | MS.gene88716:CDS |
| TGTATAAGCCGATGCCTTCT+GGG | 0.382761 | 5.4:+54193916 | MS.gene88716:CDS |
| CGCCCATAAGGATTTGGAAC+AGG | 0.390119 | 5.4:+54193583 | MS.gene88716:CDS |
| AACCTGTTCCAAATCCTTAT+GGG | 0.424723 | 5.4:-54193585 | None:intergenic |
| GCTTGTGACTGCCGCCCATA+AGG | 0.446228 | 5.4:+54193571 | MS.gene88716:CDS |
| GAAGGAGGAGGAGGTTAACA+AGG | 0.482220 | 5.4:+54193475 | MS.gene88716:CDS |
| GCGGTCGGGAACGGCGGTCG+TGG | 0.483448 | 5.4:+54193422 | MS.gene88716:CDS |
| CTCTATTAAGAAGGAGGAGG+AGG | 0.487768 | 5.4:+54193466 | MS.gene88716:CDS |
| TTATGTCTGCTCTATTAAGA+AGG | 0.518962 | 5.4:+54193457 | MS.gene88716:CDS |
| AAGCTGGAATGTTGTACCTC+GGG | 0.522913 | 5.4:-54193824 | None:intergenic |
| TTGGTCTCCCCAGAAGGCAT+CGG | 0.529922 | 5.4:-54193924 | None:intergenic |
| CTCGCAACCTATCAGAGACT+TGG | 0.530721 | 5.4:+54193536 | MS.gene88716:CDS |
| TTTCATTTGGTCTCCCCAGA+AGG | 0.542332 | 5.4:-54193930 | None:intergenic |
| TTTCACATCGCGGTCGGGAA+CGG | 0.544284 | 5.4:+54193413 | MS.gene88716:CDS |
| AGCGATACCAAGTCTCTGAT+AGG | 0.565478 | 5.4:-54193543 | None:intergenic |
| TTCATAAATATCGTAATCCA+AGG | 0.567888 | 5.4:-54193865 | None:intergenic |
| GCAAGATAAGATCATATTCG+CGG | 0.579984 | 5.4:-54193367 | None:intergenic |
| CACATCGCGGTCGGGAACGG+CGG | 0.580748 | 5.4:+54193416 | MS.gene88716:CDS |
| GGAGGAGGAGGTTAACAAGG+AGG | 0.582874 | 5.4:+54193478 | MS.gene88716:CDS |
| CTGCTCTATTAAGAAGGAGG+AGG | 0.589794 | 5.4:+54193463 | MS.gene88716:CDS |
| ACATCTTAGCGCTCGTCCCG+AGG | 0.593384 | 5.4:+54193808 | MS.gene88716:CDS |
| CACTGAAATCAGTCATATCG+TGG | 0.604541 | 5.4:+54193511 | MS.gene88716:CDS |
| ACGAGCGCTAAGATGTACTC+AGG | 0.612926 | 5.4:-54193801 | None:intergenic |
| AAAGCTGGAATGTTGTACCT+CGG | 0.618480 | 5.4:-54193825 | None:intergenic |
| TGTTCATCTCCAGAACTCAA+AGG | 0.626078 | 5.4:-54193389 | None:intergenic |
| GTATAAGCCGATGCCTTCTG+GGG | 0.633883 | 5.4:+54193917 | MS.gene88716:CDS |
| TGTCTGCTCTATTAAGAAGG+AGG | 0.648646 | 5.4:+54193460 | MS.gene88716:CDS |
| CTGTTCCAAATCCTTATGGG+CGG | 0.673914 | 5.4:-54193582 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CATATTAATTATACAAAAGT+TGG | - | chr5.4:54193717-54193736 | None:intergenic | 15.0% |
| ! | GTTCTGTAATTCTAAATGTT+AGG | + | chr5.4:54193676-54193695 | MS.gene88716:intron | 25.0% |
| ! | TTCATAAATATCGTAATCCA+AGG | - | chr5.4:54193868-54193887 | None:intergenic | 25.0% |
| !! | ACATTTAGAATTACAGAACT+AGG | - | chr5.4:54193675-54193694 | None:intergenic | 25.0% |
| !! | CATTTAGAATTACAGAACTA+GGG | - | chr5.4:54193674-54193693 | None:intergenic | 25.0% |
| TTATGTCTGCTCTATTAAGA+AGG | + | chr5.4:54193457-54193476 | MS.gene88716:CDS | 30.0% | |
| AAACCTGTTCCAAATCCTTA+TGG | - | chr5.4:54193589-54193608 | None:intergenic | 35.0% | |
| AACCTGTTCCAAATCCTTAT+GGG | - | chr5.4:54193588-54193607 | None:intergenic | 35.0% | |
| GCAAGATAAGATCATATTCG+CGG | - | chr5.4:54193370-54193389 | None:intergenic | 35.0% | |
| ! | AGATGAACATTTTCACATCG+CGG | + | chr5.4:54193403-54193422 | MS.gene88716:CDS | 35.0% |
| ! | AGCTTTCATTTTGCTCCAAA+AGG | + | chr5.4:54193842-54193861 | MS.gene88716:CDS | 35.0% |
| !!! | TTTTGGAGCAAAATGAAAGC+TGG | - | chr5.4:54193843-54193862 | None:intergenic | 35.0% |
| AAAGCTGGAATGTTGTACCT+CGG | - | chr5.4:54193828-54193847 | None:intergenic | 40.0% | |
| CACTGAAATCAGTCATATCG+TGG | + | chr5.4:54193511-54193530 | MS.gene88716:CDS | 40.0% | |
| TGTCTGCTCTATTAAGAAGG+AGG | + | chr5.4:54193460-54193479 | MS.gene88716:CDS | 40.0% | |
| TGTTCATCTCCAGAACTCAA+AGG | - | chr5.4:54193392-54193411 | None:intergenic | 40.0% | |
| ! | CTTATCTTGCCTTTGAGTTC+TGG | + | chr5.4:54193380-54193399 | MS.gene88716:CDS | 40.0% |
| ! | TATCGTAATCCAAGGCCTTT+TGG | - | chr5.4:54193860-54193879 | None:intergenic | 40.0% |
| AAGCTGGAATGTTGTACCTC+GGG | - | chr5.4:54193827-54193846 | None:intergenic | 45.0% | |
| AGCGATACCAAGTCTCTGAT+AGG | - | chr5.4:54193546-54193565 | None:intergenic | 45.0% | |
| CTCTATTAAGAAGGAGGAGG+AGG | + | chr5.4:54193466-54193485 | MS.gene88716:CDS | 45.0% | |
| CTGCTCTATTAAGAAGGAGG+AGG | + | chr5.4:54193463-54193482 | MS.gene88716:CDS | 45.0% | |
| CTGTTCCAAATCCTTATGGG+CGG | - | chr5.4:54193585-54193604 | None:intergenic | 45.0% | |
| TGTATAAGCCGATGCCTTCT+GGG | + | chr5.4:54193916-54193935 | MS.gene88716:CDS | 45.0% | |
| TTGTATAAGCCGATGCCTTC+TGG | + | chr5.4:54193915-54193934 | MS.gene88716:CDS | 45.0% | |
| ! | AACATTTTCACATCGCGGTC+GGG | + | chr5.4:54193408-54193427 | MS.gene88716:CDS | 45.0% |
| ! | CATTTTGCTCCAAAAGGCCT+TGG | + | chr5.4:54193848-54193867 | MS.gene88716:CDS | 45.0% |
| ! | GAACATTTTCACATCGCGGT+CGG | + | chr5.4:54193407-54193426 | MS.gene88716:CDS | 45.0% |
| ACGAGCGCTAAGATGTACTC+AGG | - | chr5.4:54193804-54193823 | None:intergenic | 50.0% | |
| CTCGCAACCTATCAGAGACT+TGG | + | chr5.4:54193536-54193555 | MS.gene88716:CDS | 50.0% | |
| GAAGGAGGAGGAGGTTAACA+AGG | + | chr5.4:54193475-54193494 | MS.gene88716:CDS | 50.0% | |
| GTATAAGCCGATGCCTTCTG+GGG | + | chr5.4:54193917-54193936 | MS.gene88716:CDS | 50.0% | |
| ! | CGCCCATAAGGATTTGGAAC+AGG | + | chr5.4:54193583-54193602 | MS.gene88716:CDS | 50.0% |
| GACTGCCGCCCATAAGGATT+TGG | + | chr5.4:54193577-54193596 | MS.gene88716:CDS | 55.0% | |
| GGAGGAGGAGGTTAACAAGG+AGG | + | chr5.4:54193478-54193497 | MS.gene88716:CDS | 55.0% | |
| TTTCACATCGCGGTCGGGAA+CGG | + | chr5.4:54193413-54193432 | MS.gene88716:CDS | 55.0% | |
| ! | TTGGTCTCCCCAGAAGGCAT+CGG | - | chr5.4:54193927-54193946 | None:intergenic | 55.0% |
| ACATCTTAGCGCTCGTCCCG+AGG | + | chr5.4:54193808-54193827 | MS.gene88716:CDS | 60.0% | |
| GCTTGTGACTGCCGCCCATA+AGG | + | chr5.4:54193571-54193590 | MS.gene88716:CDS | 60.0% | |
| CACATCGCGGTCGGGAACGG+CGG | + | chr5.4:54193416-54193435 | MS.gene88716:CDS | 70.0% | |
| ! | GCGGTCGGGAACGGCGGTCG+TGG | + | chr5.4:54193422-54193441 | MS.gene88716:CDS | 80.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 54193314 | 54193950 | 54193314 | ID=MS.gene88716 |
| chr5.4 | mRNA | 54193314 | 54193950 | 54193314 | ID=MS.gene88716.t1;Parent=MS.gene88716 |
| chr5.4 | exon | 54193314 | 54193604 | 54193314 | ID=MS.gene88716.t1.exon1;Parent=MS.gene88716.t1 |
| chr5.4 | CDS | 54193314 | 54193604 | 54193314 | ID=cds.MS.gene88716.t1;Parent=MS.gene88716.t1 |
| chr5.4 | exon | 54193792 | 54193950 | 54193792 | ID=MS.gene88716.t1.exon2;Parent=MS.gene88716.t1 |
| chr5.4 | CDS | 54193792 | 54193950 | 54193792 | ID=cds.MS.gene88716.t1;Parent=MS.gene88716.t1 |
| Gene Sequence |
| Protein sequence |
