Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88717.t1 | OMJ84645.1 | 40.9 | 88 | 50 | 1 | 8 | 95 | 50 | 135 | 7.00E-07 | 63.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88717.t1 | Q9FKI4 | 42.0 | 88 | 47 | 2 | 8 | 95 | 51 | 134 | 4.2e-08 | 58.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88717.t1 | A0A1R2C6T9 | 40.9 | 88 | 50 | 1 | 8 | 95 | 50 | 135 | 5.1e-07 | 63.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene88717.t1 | TR | GNAT |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88717.t1 | MTR_3g034200 | 40.909 | 88 | 48 | 2 | 8 | 95 | 51 | 134 | 2.62e-12 | 60.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88717.t1 | AT5G13780 | 42.045 | 88 | 47 | 2 | 8 | 95 | 51 | 134 | 2.92e-12 | 60.5 |
Find 29 sgRNAs with CRISPR-Local
Find 39 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGTATAAGCCGATGCCTTC+TGG | 0.236300 | 5.4:+54201619 | MS.gene88717:CDS |
| AAACCTGTTCCAAATCCTTA+TGG | 0.275073 | 5.4:-54201290 | None:intergenic |
| TATCGTAATCCAAGGCCTTT+TGG | 0.277027 | 5.4:-54201561 | None:intergenic |
| GACTGCCGCCCATAAGGATT+TGG | 0.362443 | 5.4:+54201281 | MS.gene88717:CDS |
| TGTATAAGCCGATGCCTTCT+GGG | 0.382761 | 5.4:+54201620 | MS.gene88717:CDS |
| CGCCCATAAGGATTTGGAAC+AGG | 0.390119 | 5.4:+54201287 | MS.gene88717:CDS |
| GAAGGAGGAGGAGGTTAAGA+AGG | 0.410903 | 5.4:+54201179 | MS.gene88717:CDS |
| AACCTGTTCCAAATCCTTAT+GGG | 0.424723 | 5.4:-54201289 | None:intergenic |
| GCTTGTGACTGCCGCCCATA+AGG | 0.446228 | 5.4:+54201275 | MS.gene88717:CDS |
| CTCTATTAAGAAGGAGGAGG+AGG | 0.487768 | 5.4:+54201170 | MS.gene88717:CDS |
| GCGGTCGAGAACGGCGGTCG+TGG | 0.500785 | 5.4:+54201126 | MS.gene88717:intron |
| GGAGGAGGAGGTTAAGAAGG+AGG | 0.522170 | 5.4:+54201182 | MS.gene88717:CDS |
| AAGCTGGAATGTTGTACCTC+GGG | 0.522913 | 5.4:-54201528 | None:intergenic |
| TTGGTCTCCCCAGAAGGCAT+CGG | 0.529922 | 5.4:-54201628 | None:intergenic |
| CTCGCAACCTATCAGAGACT+TGG | 0.530721 | 5.4:+54201240 | MS.gene88717:CDS |
| TTTCATTTGGTCTCCCCAGA+AGG | 0.542332 | 5.4:-54201634 | None:intergenic |
| TTATGTCTGCTCTATTAAGA+AGG | 0.559468 | 5.4:+54201161 | MS.gene88717:CDS |
| AGCGATACCAAGTCTCTGAT+AGG | 0.565478 | 5.4:-54201247 | None:intergenic |
| TTCATAAATATCGTAATCCA+AGG | 0.567888 | 5.4:-54201569 | None:intergenic |
| CTGCTCTATTAAGAAGGAGG+AGG | 0.589794 | 5.4:+54201167 | MS.gene88717:CDS |
| ACATCTTAGCGCTCGTCCCG+AGG | 0.593384 | 5.4:+54201512 | MS.gene88717:CDS |
| GGCGGTCGTGGCTATATCGT+CGG | 0.601538 | 5.4:+54201138 | MS.gene88717:CDS |
| ACGAGCGCTAAGATGTACTC+AGG | 0.612926 | 5.4:-54201505 | None:intergenic |
| AAAGCTGGAATGTTGTACCT+CGG | 0.618480 | 5.4:-54201529 | None:intergenic |
| CACTGAAATCAGTCACGTCG+TGG | 0.624295 | 5.4:+54201215 | MS.gene88717:CDS |
| CACATCGCGGTCGAGAACGG+CGG | 0.626555 | 5.4:+54201120 | MS.gene88717:intron |
| GTATAAGCCGATGCCTTCTG+GGG | 0.633883 | 5.4:+54201621 | MS.gene88717:CDS |
| TGTCTGCTCTATTAAGAAGG+AGG | 0.648646 | 5.4:+54201164 | MS.gene88717:CDS |
| CTGTTCCAAATCCTTATGGG+CGG | 0.673914 | 5.4:-54201286 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CATACTAATTATACAAAAGT+TGG | - | chr5.4:54201421-54201440 | None:intergenic | 20.0% |
| ! | GTTCTGTAATTCTAAATGTT+AGG | + | chr5.4:54201380-54201399 | MS.gene88717:intron | 25.0% |
| ! | TTCATAAATATCGTAATCCA+AGG | - | chr5.4:54201572-54201591 | None:intergenic | 25.0% |
| !! | ACATTTAGAATTACAGAACT+AGG | - | chr5.4:54201379-54201398 | None:intergenic | 25.0% |
| !! | CATTTAGAATTACAGAACTA+GGG | - | chr5.4:54201378-54201397 | None:intergenic | 25.0% |
| TTATGTCTGCTCTATTAAGA+AGG | + | chr5.4:54201161-54201180 | MS.gene88717:CDS | 30.0% | |
| AAACCTGTTCCAAATCCTTA+TGG | - | chr5.4:54201293-54201312 | None:intergenic | 35.0% | |
| AACCTGTTCCAAATCCTTAT+GGG | - | chr5.4:54201292-54201311 | None:intergenic | 35.0% | |
| GCAAGATAAGATCATATTCG+CGG | - | chr5.4:54201074-54201093 | None:intergenic | 35.0% | |
| TGTTCATCTCAAGAACTCAT+AGG | - | chr5.4:54201096-54201115 | None:intergenic | 35.0% | |
| ! | AGATGAACATTTTCACATCG+CGG | + | chr5.4:54201107-54201126 | MS.gene88717:intron | 35.0% |
| ! | AGCTTTCATTTTGCTCCAAA+AGG | + | chr5.4:54201546-54201565 | MS.gene88717:CDS | 35.0% |
| !!! | TTTTGGAGCAAAATGAAAGC+TGG | - | chr5.4:54201547-54201566 | None:intergenic | 35.0% |
| AAAGCTGGAATGTTGTACCT+CGG | - | chr5.4:54201532-54201551 | None:intergenic | 40.0% | |
| TGTCTGCTCTATTAAGAAGG+AGG | + | chr5.4:54201164-54201183 | MS.gene88717:CDS | 40.0% | |
| ! | TATCGTAATCCAAGGCCTTT+TGG | - | chr5.4:54201564-54201583 | None:intergenic | 40.0% |
| AAGCTGGAATGTTGTACCTC+GGG | - | chr5.4:54201531-54201550 | None:intergenic | 45.0% | |
| AGCGATACCAAGTCTCTGAT+AGG | - | chr5.4:54201250-54201269 | None:intergenic | 45.0% | |
| CTCTATTAAGAAGGAGGAGG+AGG | + | chr5.4:54201170-54201189 | MS.gene88717:CDS | 45.0% | |
| CTGCTCTATTAAGAAGGAGG+AGG | + | chr5.4:54201167-54201186 | MS.gene88717:CDS | 45.0% | |
| CTGTTCCAAATCCTTATGGG+CGG | - | chr5.4:54201289-54201308 | None:intergenic | 45.0% | |
| TGTATAAGCCGATGCCTTCT+GGG | + | chr5.4:54201620-54201639 | MS.gene88717:CDS | 45.0% | |
| TTGTATAAGCCGATGCCTTC+TGG | + | chr5.4:54201619-54201638 | MS.gene88717:CDS | 45.0% | |
| ! | CATTTTGCTCCAAAAGGCCT+TGG | + | chr5.4:54201552-54201571 | MS.gene88717:CDS | 45.0% |
| ACGAGCGCTAAGATGTACTC+AGG | - | chr5.4:54201508-54201527 | None:intergenic | 50.0% | |
| CACTGAAATCAGTCACGTCG+TGG | + | chr5.4:54201215-54201234 | MS.gene88717:CDS | 50.0% | |
| CTCGCAACCTATCAGAGACT+TGG | + | chr5.4:54201240-54201259 | MS.gene88717:CDS | 50.0% | |
| GAAGGAGGAGGAGGTTAAGA+AGG | + | chr5.4:54201179-54201198 | MS.gene88717:CDS | 50.0% | |
| GTATAAGCCGATGCCTTCTG+GGG | + | chr5.4:54201621-54201640 | MS.gene88717:CDS | 50.0% | |
| TTTCACATCGCGGTCGAGAA+CGG | + | chr5.4:54201117-54201136 | MS.gene88717:intron | 50.0% | |
| ! | CGCCCATAAGGATTTGGAAC+AGG | + | chr5.4:54201287-54201306 | MS.gene88717:CDS | 50.0% |
| GACTGCCGCCCATAAGGATT+TGG | + | chr5.4:54201281-54201300 | MS.gene88717:CDS | 55.0% | |
| GGAGGAGGAGGTTAAGAAGG+AGG | + | chr5.4:54201182-54201201 | MS.gene88717:CDS | 55.0% | |
| ! | TTGGTCTCCCCAGAAGGCAT+CGG | - | chr5.4:54201631-54201650 | None:intergenic | 55.0% |
| ACATCTTAGCGCTCGTCCCG+AGG | + | chr5.4:54201512-54201531 | MS.gene88717:CDS | 60.0% | |
| GCTTGTGACTGCCGCCCATA+AGG | + | chr5.4:54201275-54201294 | MS.gene88717:CDS | 60.0% | |
| GGCGGTCGTGGCTATATCGT+CGG | + | chr5.4:54201138-54201157 | MS.gene88717:CDS | 60.0% | |
| CACATCGCGGTCGAGAACGG+CGG | + | chr5.4:54201120-54201139 | MS.gene88717:intron | 65.0% | |
| GCGGTCGAGAACGGCGGTCG+TGG | + | chr5.4:54201126-54201145 | MS.gene88717:intron | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 54201018 | 54201654 | 54201018 | ID=MS.gene88717 |
| chr5.4 | mRNA | 54201018 | 54201654 | 54201018 | ID=MS.gene88717.t1;Parent=MS.gene88717 |
| chr5.4 | exon | 54201018 | 54201026 | 54201018 | ID=MS.gene88717.t1.exon1;Parent=MS.gene88717.t1 |
| chr5.4 | CDS | 54201018 | 54201026 | 54201018 | ID=cds.MS.gene88717.t1;Parent=MS.gene88717.t1 |
| chr5.4 | exon | 54201135 | 54201308 | 54201135 | ID=MS.gene88717.t1.exon2;Parent=MS.gene88717.t1 |
| chr5.4 | CDS | 54201135 | 54201308 | 54201135 | ID=cds.MS.gene88717.t1;Parent=MS.gene88717.t1 |
| chr5.4 | exon | 54201496 | 54201654 | 54201496 | ID=MS.gene88717.t1.exon3;Parent=MS.gene88717.t1 |
| chr5.4 | CDS | 54201496 | 54201654 | 54201496 | ID=cds.MS.gene88717.t1;Parent=MS.gene88717.t1 |
| Gene Sequence |
| Protein sequence |
