AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene89023

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene89023.t1 MTR_1g070355 46.296 270 141 2 1 269 1 267 7.08e-77 252
MS.gene89023.t1 MTR_5g076070 36.170 188 120 0 81 268 58 245 1.13e-39 145
MS.gene89023.t1 MTR_6g022080 34.783 184 120 0 57 240 66 249 2.75e-38 140
MS.gene89023.t1 MTR_2g101460 48.592 142 69 1 40 181 23 160 7.37e-34 127
MS.gene89023.t1 MTR_2g101460 48.592 142 69 1 40 181 13 150 1.20e-33 126
MS.gene89023.t1 MTR_0060s0090 51.163 129 43 2 35 163 35 143 1.43e-33 124
MS.gene89023.t1 MTR_6g463250 48.507 134 48 2 35 162 41 159 1.59e-32 122
MS.gene89023.t1 MTR_7g446520 29.699 266 157 6 8 266 20 262 1.30e-27 113
MS.gene89023.t1 MTR_3g448650 46.429 112 56 1 40 151 50 157 1.12e-26 106
MS.gene89023.t1 MTR_1g052260 44.231 104 58 0 55 158 55 158 4.41e-25 101
MS.gene89023.t1 MTR_4g050510 38.583 127 64 1 87 213 89 201 3.64e-23 97.8
MS.gene89023.t1 MTR_6g053140 37.324 142 79 3 31 169 55 189 1.16e-21 93.6
MS.gene89023.t1 MTR_7g028855 29.148 223 150 5 3 219 43 263 1.56e-19 91.7
MS.gene89023.t1 MTR_8g066350 33.858 127 66 1 55 181 58 166 1.35e-17 80.9
MS.gene89023.t1 MTR_0402s0040 32.143 168 106 5 80 245 32 193 1.95e-16 82.0
MS.gene89023.t1 MTR_7g446480 36.207 116 72 1 153 266 140 255 6.25e-15 76.3
MS.gene89023.t1 MTR_0604s0020 41.892 74 43 0 87 160 3 76 1.96e-14 68.9
MS.gene89023.t1 MTR_7g446510 36.207 116 72 1 153 266 155 270 1.39e-13 72.4
MS.gene89023.t1 MTR_3g450850 29.885 174 89 6 86 228 57 228 1.16e-12 69.7
MS.gene89023.t1 MTR_6g051425 28.061 196 129 6 86 273 27 218 2.33e-11 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene89023.t1 AT4G38180 29.796 245 158 8 80 317 77 314 9.16e-18 87.0
MS.gene89023.t1 AT5G28530 33.750 160 102 3 82 238 61 219 1.19e-16 83.2
MS.gene89023.t1 AT5G28530 33.750 160 102 3 82 238 61 219 1.19e-16 83.2
MS.gene89023.t1 AT5G28530 33.750 160 102 3 82 238 61 219 1.40e-16 83.2
MS.gene89023.t1 AT5G28530 33.750 160 102 3 82 238 61 219 1.61e-16 83.2
MS.gene89023.t1 AT5G28530 33.750 160 102 3 82 238 61 219 1.61e-16 83.2
MS.gene89023.t1 AT1G10240 28.516 256 161 9 82 318 54 306 8.90e-16 80.9
MS.gene89023.t1 AT1G10240 28.516 256 161 9 82 318 54 306 8.90e-16 80.9

Find 103 sgRNAs with CRISPR-Local

Find 177 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCACACCTGGTATCATTTC+TGG 0.155992 2.4:+1820795 None:intergenic
CACAAATGGTTCACACTATT+GGG 0.270384 2.4:-1819309 MS.gene89023:CDS
ACACAAATGGTTCACACTAT+TGG 0.283603 2.4:-1819310 MS.gene89023:CDS
ATTTCAAATGTTCGTGTCTT+AGG 0.296750 2.4:-1819463 MS.gene89023:CDS
GGGAATGCTATAGTCATAAA+TGG 0.304640 2.4:-1821066 MS.gene89023:CDS
GTACTTTGAAACTTTGTAGA+TGG 0.321924 2.4:+1819535 None:intergenic
TCACCGTTGTTGACTCCTTT+AGG 0.330711 2.4:+1818963 None:intergenic
GTATGTGCAACATACATTAA+AGG 0.333195 2.4:+1820427 None:intergenic
CCTGTGTAAAACTAGATTCT+TGG 0.335728 2.4:+1819370 None:intergenic
ATTGCACCATACGTTTGATA+TGG 0.351243 2.4:+1820589 None:intergenic
CCAAGTTCTGAGGGACCGTT+TGG 0.359223 2.4:-1820905 MS.gene89023:CDS
CCAAACGGTCCCTCAGAACT+TGG 0.376804 2.4:+1820905 None:intergenic
TTCAGACGAAGGAGATAATA+TGG 0.381516 2.4:-1821154 MS.gene89023:CDS
CTTCCACCAGGCGTAGCAAT+TGG 0.387040 2.4:+1819044 None:intergenic
GTTTCAAAGTACTGTGGAAA+TGG 0.394957 2.4:-1819524 MS.gene89023:CDS
CCACACCTGGTATCATTTCT+GGG 0.408982 2.4:+1820796 None:intergenic
AGCGATTCATCCGAGTCTAA+TGG 0.410491 2.4:-1821087 MS.gene89023:CDS
AGTCGTCGACGAAAGTTTAC+TGG 0.423532 2.4:-1819105 MS.gene89023:CDS
TATGTGCAACATACATTAAA+GGG 0.429560 2.4:+1820428 None:intergenic
TCATGGTATGCAAGGATAAA+CGG 0.431197 2.4:-1820946 MS.gene89023:CDS
AAGGATTTGTATAATCAATC+AGG 0.433589 2.4:-1820523 MS.gene89023:CDS
GAGATAATATGGATGACAAT+GGG 0.436783 2.4:-1821143 MS.gene89023:CDS
GGGATTCTCAAATTGCTTCA+AGG 0.437802 2.4:-1819289 MS.gene89023:CDS
CTGTGTAAAACTAGATTCTT+GGG 0.438174 2.4:+1819371 None:intergenic
CCTGCTTGTGTGAGGACTAA+AGG 0.439894 2.4:-1819080 MS.gene89023:CDS
GCGATTCATCCGAGTCTAAT+GGG 0.449731 2.4:-1821086 MS.gene89023:CDS
TGTCTTAGGATGGAGTCGTT+TGG 0.452899 2.4:-1819449 MS.gene89023:CDS
CACGAACATTTGAAATCTAC+AGG 0.467229 2.4:+1819470 None:intergenic
CTGCTTGTGTGAGGACTAAA+GGG 0.473570 2.4:-1819079 MS.gene89023:CDS
TGTAGTCAGTGTGGTGTGAT+AGG 0.474905 2.4:-1819005 MS.gene89023:CDS
TATTCAGTTGTCGAAGGATG+TGG 0.485194 2.4:-1819255 MS.gene89023:CDS
GTTGGTATTGATGCTAGCAC+TGG 0.485843 2.4:-1819140 MS.gene89023:CDS
TTCTCGCTTCCGCCTCTCCA+AGG 0.489867 2.4:+1820819 None:intergenic
CTGTTGGATGGTTTGCTATG+TGG 0.491215 2.4:-1820688 MS.gene89023:CDS
GGTGCAATGGCTAATTCATC+AGG 0.491612 2.4:-1820574 MS.gene89023:CDS
TCAATCTCAATCAAATCAGT+TGG 0.493229 2.4:+1820637 None:intergenic
CCTTTAGTCCTCACACAAGC+AGG 0.493667 2.4:+1819080 None:intergenic
TTGCATATAATTTCTACTCA+TGG 0.494106 2.4:-1820963 MS.gene89023:CDS
AGAGAACAGGGGATTGACCT+TGG 0.494325 2.4:-1820836 MS.gene89023:CDS
TTGCTACGCCTGGTGGAAGT+AGG 0.503186 2.4:-1819040 MS.gene89023:CDS
CAATATTAACATCGAGGGAA+TGG 0.503955 2.4:-1821184 MS.gene89023:CDS
CAAATTGCTTCAAGGTATGC+GGG 0.516691 2.4:-1819281 MS.gene89023:CDS
CAAATGTTCGTGTCTTAGGA+TGG 0.523067 2.4:-1819459 MS.gene89023:CDS
AATAAACGATCATGTCCTAA+AGG 0.523227 2.4:-1818978 MS.gene89023:CDS
ATTAATGAAGATGATGAAGA+AGG 0.525000 2.4:-1818906 MS.gene89023:CDS
GTCGAAGGATGTGGCTGAGA+GGG 0.529678 2.4:-1819246 MS.gene89023:CDS
GTTATGCGCAAATTCAAATA+CGG 0.530100 2.4:+1820717 None:intergenic
TCTAATCCAATTGCTACGCC+TGG 0.533380 2.4:-1819050 MS.gene89023:CDS
AAGAATAAAACAACAGCTGT+TGG 0.534950 2.4:-1819158 MS.gene89023:CDS
TAGACATTGTTTCGAAGCGT+TGG 0.536274 2.4:-1820747 MS.gene89023:CDS
CAAGTTCTGAGGGACCGTTT+GGG 0.542763 2.4:-1820904 MS.gene89023:CDS
GGTGTTAGCTTTCTATGTGC+CGG 0.543539 2.4:+1820658 None:intergenic
TTGGTATTGATGCTAGCACT+GGG 0.551813 2.4:-1819139 MS.gene89023:CDS
AATCCAATTGCTACGCCTGG+TGG 0.558681 2.4:-1819047 MS.gene89023:CDS
ATTGAAAAGTATAAGAATGT+TGG 0.559478 2.4:-1820619 MS.gene89023:CDS
TGCGCATAACCATGAGCTGT+TGG 0.562938 2.4:-1820704 MS.gene89023:CDS
GCTGCAGAACATTACCCAAA+CGG 0.563552 2.4:+1820890 None:intergenic
AACTTTGTAGATGGAACCCA+TGG 0.564415 2.4:+1819544 None:intergenic
TGAAATTCAAGAGATGTCCA+TGG 0.566346 2.4:-1819561 MS.gene89023:CDS
GTTCGAGCAAGATACACAAA+TGG 0.566756 2.4:-1819323 MS.gene89023:CDS
ATTTCTACTCATGGTATGCA+AGG 0.568185 2.4:-1820954 MS.gene89023:CDS
GGAGATAATATGGATGACAA+TGG 0.571879 2.4:-1821144 MS.gene89023:CDS
CATAACCATGAGCTGTTGGA+TGG 0.572402 2.4:-1820700 MS.gene89023:CDS
TCTGTTGTAGGTTATGGAGG+AGG 0.573234 2.4:-1821235 None:intergenic
TTCGGAAGACCCAAGTTCTG+AGG 0.573488 2.4:-1820915 MS.gene89023:CDS
TACTGGACCCTGCTTGTGTG+AGG 0.573861 2.4:-1819088 MS.gene89023:CDS
TCAAATTGCTTCAAGGTATG+CGG 0.574162 2.4:-1819282 MS.gene89023:CDS
TTTCTGTAGATGTAGTTGAA+AGG 0.575027 2.4:-1821008 MS.gene89023:CDS
ATATCAAACGTATGGTGCAA+TGG 0.580935 2.4:-1820587 MS.gene89023:CDS
CCACACTGACTACATGATTG+AGG 0.582317 2.4:+1819014 None:intergenic
GAGAACCCAGAAATGATACC+AGG 0.586155 2.4:-1820801 MS.gene89023:CDS
GTAAGACCATATCAAACGTA+TGG 0.586746 2.4:-1820595 MS.gene89023:CDS
GCAAACCATCCAACAGCTCA+TGG 0.591376 2.4:+1820695 None:intergenic
GGGGATTGACCTTGGAGAGG+CGG 0.591821 2.4:-1820828 MS.gene89023:CDS
GCTATAGTCATAAATGGCAT+CGG 0.596967 2.4:-1821060 MS.gene89023:CDS
GTAAAACTAGATTCTTGGGA+AGG 0.598080 2.4:+1819375 None:intergenic
AATGACAATATTAACATCGA+GGG 0.600868 2.4:-1821189 MS.gene89023:CDS
GGGAATTATTCAGTTGTCGA+AGG 0.603195 2.4:-1819261 MS.gene89023:CDS
GAAATTCAAGAGATGTCCAT+GGG 0.603913 2.4:-1819560 MS.gene89023:CDS
TCGGAAGACCCAAGTTCTGA+GGG 0.617413 2.4:-1820914 MS.gene89023:CDS
TTAGTTTCATCTGACTCTTG+CGG 0.617850 2.4:-1820487 MS.gene89023:CDS
ACAGCAATAATATGATCGCA+AGG 0.619104 2.4:+1819422 None:intergenic
CAAACGGTCCCTCAGAACTT+GGG 0.622569 2.4:+1820906 None:intergenic
GCAATGGCTAATTCATCAGG+TGG 0.625760 2.4:-1820571 MS.gene89023:CDS
ATGGACGAAGCTTCAGACGA+AGG 0.631163 2.4:-1821165 MS.gene89023:CDS
TACAAAGTTTCAAAGTACTG+TGG 0.634037 2.4:-1819530 MS.gene89023:CDS
TGTGTGAGGACTAAAGGGTG+TGG 0.637446 2.4:-1819074 MS.gene89023:CDS
ACTGTGGAAATGGAAGTGTC+TGG 0.638067 2.4:-1819514 MS.gene89023:CDS
TATAGCATTCCCATTAGACT+CGG 0.641750 2.4:+1821077 None:intergenic
GCTTCGAAACAATGTCTATG+TGG 0.645082 2.4:+1820752 None:intergenic
AATGGGGCTAATGAGTGTGA+TGG 0.645283 2.4:-1821126 MS.gene89023:CDS
CCCAGAAATGATACCAGGTG+TGG 0.648094 2.4:-1820796 MS.gene89023:CDS
TGTCGAAGGATGTGGCTGAG+AGG 0.652941 2.4:-1819247 MS.gene89023:CDS
CTTTAGTCCTCACACAAGCA+GGG 0.655658 2.4:+1819081 None:intergenic
ATAATCAATCAGGAAGACAA+AGG 0.657924 2.4:-1820513 MS.gene89023:CDS
ACAGGGGATTGACCTTGGAG+AGG 0.670051 2.4:-1820831 MS.gene89023:CDS
TGAGGACGCCTACTTCCACC+AGG 0.673688 2.4:+1819032 None:intergenic
AGATAATATGGATGACAATG+GGG 0.688595 2.4:-1821142 MS.gene89023:CDS
CCTCAATCATGTAGTCAGTG+TGG 0.690892 2.4:-1819014 MS.gene89023:CDS
TAATGACAATATTAACATCG+AGG 0.697606 2.4:-1821190 MS.gene89023:CDS
CACTAAGAAAATCTACAATG+TGG 0.701999 2.4:+1819198 None:intergenic
TGTCCTAAAGGAGTCAACAA+CGG 0.735045 2.4:-1818966 MS.gene89023:CDS
TGGTATTGATGCTAGCACTG+GGG 0.762460 2.4:-1819138 MS.gene89023:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTGAAAAGTATAAGAATGT+TGG - chr2.4:1819493-1819512 MS.gene89023:CDS 20.0%
!!! ATTGTATTTGTTTTTCCTAT+AGG + chr2.4:1819809-1819828 None:intergenic 20.0%
!!! CGTATGAATTATGAAATTTT+TGG - chr2.4:1819760-1819779 MS.gene89023:intron 20.0%
!!! TTTTGGAAATATACTCTTTA+TGG + chr2.4:1820013-1820032 None:intergenic 20.0%
! AAGGATTTGTATAATCAATC+AGG - chr2.4:1819589-1819608 MS.gene89023:intron 25.0%
! AATGACAATATTAACATCGA+GGG - chr2.4:1818923-1818942 MS.gene89023:CDS 25.0%
! ATATCTTAAAAGATGCATGT+TGG - chr2.4:1820107-1820126 MS.gene89023:intron 25.0%
! ATTAATGAAGATGATGAAGA+AGG - chr2.4:1821206-1821225 MS.gene89023:CDS 25.0%
! GAATAAGTTTGAAGAACTAT+GGG - chr2.4:1820143-1820162 MS.gene89023:intron 25.0%
! GTATGGAAAAAAAAGAATGT+GGG - chr2.4:1820218-1820237 MS.gene89023:intron 25.0%
! TAATGACAATATTAACATCG+AGG - chr2.4:1818922-1818941 MS.gene89023:CDS 25.0%
! TATCTTAAAAGATGCATGTT+GGG - chr2.4:1820108-1820127 MS.gene89023:intron 25.0%
! TATGTGCAACATACATTAAA+GGG + chr2.4:1819687-1819706 None:intergenic 25.0%
! TGAATAAGTTTGAAGAACTA+TGG - chr2.4:1820142-1820161 MS.gene89023:intron 25.0%
! TGTATGGAAAAAAAAGAATG+TGG - chr2.4:1820217-1820236 MS.gene89023:intron 25.0%
! TTGCATATAATTTCTACTCA+TGG - chr2.4:1819149-1819168 MS.gene89023:CDS 25.0%
!!! TGAAATTTTTGGTGATGTAT+TGG - chr2.4:1819771-1819790 MS.gene89023:intron 25.0%
!!! TTTTCAAAGCATTACGAAAA+AGG + chr2.4:1820509-1820528 None:intergenic 25.0%
AAGAATAAAACAACAGCTGT+TGG - chr2.4:1820954-1820973 MS.gene89023:CDS 30.0%
AATAAACGATCATGTCCTAA+AGG - chr2.4:1821134-1821153 MS.gene89023:CDS 30.0%
AATTGGATCAATGAGATGTA+TGG - chr2.4:1820201-1820220 MS.gene89023:intron 30.0%
AGATAATATGGATGACAATG+GGG - chr2.4:1818970-1818989 MS.gene89023:CDS 30.0%
ATAATCAATCAGGAAGACAA+AGG - chr2.4:1819599-1819618 MS.gene89023:intron 30.0%
ATACTGTAGACAAAGATAAG+AGG - chr2.4:1819704-1819723 MS.gene89023:intron 30.0%
ATTTCAAATGTTCGTGTCTT+AGG - chr2.4:1820649-1820668 MS.gene89023:CDS 30.0%
CACTAAGAAAATCTACAATG+TGG + chr2.4:1820917-1820936 None:intergenic 30.0%
CTCGAAATCGAAAATATGTA+AGG + chr2.4:1820380-1820399 None:intergenic 30.0%
CTGTGTAAAACTAGATTCTT+GGG + chr2.4:1820744-1820763 None:intergenic 30.0%
GAAGAGATGGTTGAAAAATT+TGG - chr2.4:1820165-1820184 MS.gene89023:intron 30.0%
GAGATAATATGGATGACAAT+GGG - chr2.4:1818969-1818988 MS.gene89023:CDS 30.0%
GTATGTGCAACATACATTAA+AGG + chr2.4:1819688-1819707 None:intergenic 30.0%
GTTATGCGCAAATTCAAATA+CGG + chr2.4:1819398-1819417 None:intergenic 30.0%
TAATTCTTGAATGCACAAAC+TGG + chr2.4:1820323-1820342 None:intergenic 30.0%
TCAATCTCAATCAAATCAGT+TGG + chr2.4:1819478-1819497 None:intergenic 30.0%
TTGGTTATGATGATTAACAC+CGG + chr2.4:1819851-1819870 None:intergenic 30.0%
TTTCTGTAGATGTAGTTGAA+AGG - chr2.4:1819104-1819123 MS.gene89023:CDS 30.0%
! ATTTTGTCCAGCAATTTCAT+AGG - chr2.4:1820352-1820371 MS.gene89023:intron 30.0%
! CATAATCTATGATGTGCTTT+TGG + chr2.4:1820030-1820049 None:intergenic 30.0%
!! CAAGTTAGGTTTTGTGAAAA+AGG - chr2.4:1819570-1819589 MS.gene89023:CDS 30.0%
!! CATCGGTGATATTTTAAAGA+TGG - chr2.4:1819069-1819088 MS.gene89023:CDS 30.0%
!! GTACTTTGAAACTTTGTAGA+TGG + chr2.4:1820580-1820599 None:intergenic 30.0%
!! TACAAAGTTTCAAAGTACTG+TGG - chr2.4:1820582-1820601 MS.gene89023:CDS 30.0%
!!! ATCAAAGGAGAATTTTTTGC+AGG - chr2.4:1820252-1820271 MS.gene89023:intron 30.0%
!!! TATTGCTGTTTTGATGTATC+TGG - chr2.4:1820704-1820723 MS.gene89023:CDS 30.0%
AACATCACCGTCAGTTATTA+CGG + chr2.4:1819977-1819996 None:intergenic 35.0%
ACACAAATGGTTCACACTAT+TGG - chr2.4:1820802-1820821 MS.gene89023:CDS 35.0%
ACAGCAATAATATGATCGCA+AGG + chr2.4:1820693-1820712 None:intergenic 35.0%
ACCATAGTTGGAATGATAGT+CGG + chr2.4:1820412-1820431 None:intergenic 35.0%
AGAAAATCTCCCTTGTAAAC+TGG + chr2.4:1820485-1820504 None:intergenic 35.0%
AGAGACATATGTTTGGTTGT+TGG - chr2.4:1819912-1819931 MS.gene89023:intron 35.0%
ATATCAAACGTATGGTGCAA+TGG - chr2.4:1819525-1819544 MS.gene89023:CDS 35.0%
ATTGCACCATACGTTTGATA+TGG + chr2.4:1819526-1819545 None:intergenic 35.0%
ATTTCTACTCATGGTATGCA+AGG - chr2.4:1819158-1819177 MS.gene89023:CDS 35.0%
CAATATTAACATCGAGGGAA+TGG - chr2.4:1818928-1818947 MS.gene89023:CDS 35.0%
CACAAATGGTTCACACTATT+GGG - chr2.4:1820803-1820822 MS.gene89023:CDS 35.0%
CACATCATAGATTATGTGCT+TGG - chr2.4:1820034-1820053 MS.gene89023:intron 35.0%
CCTGTGTAAAACTAGATTCT+TGG + chr2.4:1820745-1820764 None:intergenic 35.0%
CCTTTATCCGTAATAACTGA+CGG - chr2.4:1819967-1819986 MS.gene89023:intron 35.0%
GAAATTCAAGAGATGTCCAT+GGG - chr2.4:1820552-1820571 MS.gene89023:CDS 35.0%
GCTATAGTCATAAATGGCAT+CGG - chr2.4:1819052-1819071 MS.gene89023:CDS 35.0%
GGAGATAATATGGATGACAA+TGG - chr2.4:1818968-1818987 MS.gene89023:CDS 35.0%
GGGAATGCTATAGTCATAAA+TGG - chr2.4:1819046-1819065 MS.gene89023:CDS 35.0%
GTAAAACTAGATTCTTGGGA+AGG + chr2.4:1820740-1820759 None:intergenic 35.0%
GTAAGACCATATCAAACGTA+TGG - chr2.4:1819517-1819536 MS.gene89023:CDS 35.0%
TATAGCATTCCCATTAGACT+CGG + chr2.4:1819038-1819057 None:intergenic 35.0%
TCAAATTGCTTCAAGGTATG+CGG - chr2.4:1820830-1820849 MS.gene89023:CDS 35.0%
TCATGGTATGCAAGGATAAA+CGG - chr2.4:1819166-1819185 MS.gene89023:CDS 35.0%
TGAAATTCAAGAGATGTCCA+TGG - chr2.4:1820551-1820570 MS.gene89023:CDS 35.0%
TTAGTTTCATCTGACTCTTG+CGG - chr2.4:1819625-1819644 MS.gene89023:intron 35.0%
TTCAGACGAAGGAGATAATA+TGG - chr2.4:1818958-1818977 MS.gene89023:CDS 35.0%
TTTCCTATAGGTTGCATCAA+AGG + chr2.4:1819797-1819816 None:intergenic 35.0%
! CACGAACATTTGAAATCTAC+AGG + chr2.4:1820645-1820664 None:intergenic 35.0%
! TCGCAAACTAGTTTGCAATA+CGG + chr2.4:1820439-1820458 None:intergenic 35.0%
!! AGCAAACTTTTGTTTGTTCG+CGG - chr2.4:1819242-1819261 MS.gene89023:CDS 35.0%
!! GGATAAACGGATTTTCTGTT+CGG - chr2.4:1819179-1819198 MS.gene89023:CDS 35.0%
!! GTTTCAAAGTACTGTGGAAA+TGG - chr2.4:1820588-1820607 MS.gene89023:CDS 35.0%
!! TTCTTAGTGCTGAACTTTTG+AGG - chr2.4:1820928-1820947 MS.gene89023:CDS 35.0%
!! TTTGCTATGTGGTCTTTTAC+CGG - chr2.4:1819435-1819454 MS.gene89023:CDS 35.0%
!!! AGAATCTAGTTTTACACAGG+TGG - chr2.4:1820745-1820764 MS.gene89023:CDS 35.0%
!!! CCAAGAATCTAGTTTTACAC+AGG - chr2.4:1820742-1820761 MS.gene89023:CDS 35.0%
!!! TGCCTGTTTGTTATTTTCTC+CGG - chr2.4:1819829-1819848 MS.gene89023:intron 35.0%
AACTTTGTAGATGGAACCCA+TGG + chr2.4:1820571-1820590 None:intergenic 40.0%
AAGATGCATGTTGGGTGATA+TGG - chr2.4:1820116-1820135 MS.gene89023:intron 40.0%
AGACGGAAGAGACATATGTT+TGG - chr2.4:1819905-1819924 MS.gene89023:intron 40.0%
AGCAACGAACCAGTTTACAA+GGG - chr2.4:1820473-1820492 MS.gene89023:CDS 40.0%
AGGTTTAGTTACGAGAGAGA+CGG - chr2.4:1819888-1819907 MS.gene89023:intron 40.0%
CAAATGTTCGTGTCTTAGGA+TGG - chr2.4:1820653-1820672 MS.gene89023:CDS 40.0%
CAAATTGCTTCAAGGTATGC+GGG - chr2.4:1820831-1820850 MS.gene89023:CDS 40.0%
CACCGGAGAAAATAACAAAC+AGG + chr2.4:1819834-1819853 None:intergenic 40.0%
CCGTCAGTTATTACGGATAA+AGG + chr2.4:1819970-1819989 None:intergenic 40.0%
GAGGGTTTACATAGTCACAT+AGG - chr2.4:1820297-1820316 MS.gene89023:intron 40.0%
GCTTCGAAACAATGTCTATG+TGG + chr2.4:1819363-1819382 None:intergenic 40.0%
GGAATGCATCATCGAATGTT+GGG - chr2.4:1820067-1820086 MS.gene89023:intron 40.0%
GGGAATTATTCAGTTGTCGA+AGG - chr2.4:1820851-1820870 MS.gene89023:CDS 40.0%
GGGATTCTCAAATTGCTTCA+AGG - chr2.4:1820823-1820842 MS.gene89023:CDS 40.0%
GTTCGAGCAAGATACACAAA+TGG - chr2.4:1820789-1820808 MS.gene89023:CDS 40.0%
TAGACATTGTTTCGAAGCGT+TGG - chr2.4:1819365-1819384 MS.gene89023:CDS 40.0%
TATGTGCTTGGCATCTTCTA+CGG - chr2.4:1820046-1820065 MS.gene89023:intron 40.0%
TATTCAGTTGTCGAAGGATG+TGG - chr2.4:1820857-1820876 MS.gene89023:CDS 40.0%
TCCGACTATCATTCCAACTA+TGG - chr2.4:1820408-1820427 MS.gene89023:intron 40.0%
TGAAGAACTATGGGAAGAGA+TGG - chr2.4:1820152-1820171 MS.gene89023:intron 40.0%
TGTCCTAAAGGAGTCAACAA+CGG - chr2.4:1821146-1821165 MS.gene89023:CDS 40.0%
TTGGTCTGCAAGACAACAAT+TGG - chr2.4:1820184-1820203 MS.gene89023:intron 40.0%
! CGCAAACTAGTTTGCAATAC+GGG + chr2.4:1820438-1820457 None:intergenic 40.0%
! TAAGGCACCTATGAAATTGC+TGG + chr2.4:1820362-1820381 None:intergenic 40.0%
! TCAGGTGGTTTTCACAAGTT+AGG - chr2.4:1819556-1819575 MS.gene89023:CDS 40.0%
!! GCAAACTTTTGTTTGTTCGC+GGG - chr2.4:1819243-1819262 MS.gene89023:CDS 40.0%
!! TTGGTATTGATGCTAGCACT+GGG - chr2.4:1820973-1820992 MS.gene89023:CDS 40.0%
!!! AGAGGCTTGAACGTCTTTTT+TGG - chr2.4:1819722-1819741 MS.gene89023:intron 40.0%
!!! GAACGTCTTTTTTGGTGTGA+TGG - chr2.4:1819730-1819749 MS.gene89023:intron 40.0%
!!! GAAGGTTTTAGAGAGAACAG+GGG - chr2.4:1819265-1819284 MS.gene89023:CDS 40.0%
!!! GGAAGGTTTTAGAGAGAACA+GGG - chr2.4:1819264-1819283 MS.gene89023:CDS 40.0%
AATGGGGCTAATGAGTGTGA+TGG - chr2.4:1818986-1819005 MS.gene89023:CDS 45.0%
AGCGATTCATCCGAGTCTAA+TGG - chr2.4:1819025-1819044 MS.gene89023:CDS 45.0%
AGTCGTCGACGAAAGTTTAC+TGG - chr2.4:1821007-1821026 MS.gene89023:CDS 45.0%
CAAACTGTTGTCTTTGCGAC+AGG - chr2.4:1819868-1819887 MS.gene89023:intron 45.0%
CAATTCGCGACTGCTATGAA+TGG - chr2.4:1819937-1819956 MS.gene89023:intron 45.0%
CAGCAACGAACCAGTTTACA+AGG - chr2.4:1820472-1820491 MS.gene89023:CDS 45.0%
CATAACCATGAGCTGTTGGA+TGG - chr2.4:1819412-1819431 MS.gene89023:CDS 45.0%
CATTCATAGCAGTCGCGAAT+TGG + chr2.4:1819939-1819958 None:intergenic 45.0%
CCACACCTGGTATCATTTCT+GGG + chr2.4:1819319-1819338 None:intergenic 45.0%
CCTCAATCATGTAGTCAGTG+TGG - chr2.4:1821098-1821117 MS.gene89023:CDS 45.0%
CGGAATGCATCATCGAATGT+TGG - chr2.4:1820066-1820085 MS.gene89023:intron 45.0%
CTGCTTGTGTGAGGACTAAA+GGG - chr2.4:1821033-1821052 MS.gene89023:CDS 45.0%
CTGTCGCAAAGACAACAGTT+TGG + chr2.4:1819870-1819889 None:intergenic 45.0%
CTTTAGTCCTCACACAAGCA+GGG + chr2.4:1821034-1821053 None:intergenic 45.0%
GAGAACCCAGAAATGATACC+AGG - chr2.4:1819311-1819330 MS.gene89023:CDS 45.0%
GCAATGGCTAATTCATCAGG+TGG - chr2.4:1819541-1819560 MS.gene89023:CDS 45.0%
GCGATTCATCCGAGTCTAAT+GGG - chr2.4:1819026-1819045 MS.gene89023:CDS 45.0%
GCTGCAGAACATTACCCAAA+CGG + chr2.4:1819225-1819244 None:intergenic 45.0%
GGTGCAATGGCTAATTCATC+AGG - chr2.4:1819538-1819557 MS.gene89023:CDS 45.0%
TCCACACCTGGTATCATTTC+TGG + chr2.4:1819320-1819339 None:intergenic 45.0%
TCTAATCCAATTGCTACGCC+TGG - chr2.4:1821062-1821081 MS.gene89023:CDS 45.0%
TGGCCTTTGATGCAACCTAT+AGG - chr2.4:1819791-1819810 MS.gene89023:intron 45.0%
TGTAGTCAGTGTGGTGTGAT+AGG - chr2.4:1821107-1821126 MS.gene89023:CDS 45.0%
TGTCTTAGGATGGAGTCGTT+TGG - chr2.4:1820663-1820682 MS.gene89023:CDS 45.0%
! CAATACGGGTTGACCATAGT+TGG + chr2.4:1820424-1820443 None:intergenic 45.0%
! CCACACTGACTACATGATTG+AGG + chr2.4:1821101-1821120 None:intergenic 45.0%
! TTTTGCAGAACATCCACACC+TGG + chr2.4:1819332-1819351 None:intergenic 45.0%
!! ACTGTGGAAATGGAAGTGTC+TGG - chr2.4:1820598-1820617 MS.gene89023:CDS 45.0%
!! ACTTTTGTTTGTTCGCGGGA+AGG - chr2.4:1819247-1819266 MS.gene89023:CDS 45.0%
!! CTGTTGGATGGTTTGCTATG+TGG - chr2.4:1819424-1819443 MS.gene89023:CDS 45.0%
!! GGTGTTAGCTTTCTATGTGC+CGG + chr2.4:1819457-1819476 None:intergenic 45.0%
!! GTTGGTATTGATGCTAGCAC+TGG - chr2.4:1820972-1820991 MS.gene89023:CDS 45.0%
!! TCACCGTTGTTGACTCCTTT+AGG + chr2.4:1821152-1821171 None:intergenic 45.0%
!! TGGTATTGATGCTAGCACTG+GGG - chr2.4:1820974-1820993 MS.gene89023:CDS 45.0%
!!! GGGAAGGTTTTAGAGAGAAC+AGG - chr2.4:1819263-1819282 MS.gene89023:CDS 45.0%
AATCCAATTGCTACGCCTGG+TGG - chr2.4:1821065-1821084 MS.gene89023:CDS 50.0%
AGAGAACAGGGGATTGACCT+TGG - chr2.4:1819276-1819295 MS.gene89023:CDS 50.0%
ATGGACGAAGCTTCAGACGA+AGG - chr2.4:1818947-1818966 MS.gene89023:CDS 50.0%
CAAACGGTCCCTCAGAACTT+GGG + chr2.4:1819209-1819228 None:intergenic 50.0%
CAGGTATACGAACCACGTCT+CGG - chr2.4:1820271-1820290 MS.gene89023:intron 50.0%
CCCAGAAATGATACCAGGTG+TGG - chr2.4:1819316-1819335 MS.gene89023:CDS 50.0%
CCTGCTTGTGTGAGGACTAA+AGG - chr2.4:1821032-1821051 MS.gene89023:CDS 50.0%
CCTTTAGTCCTCACACAAGC+AGG + chr2.4:1821035-1821054 None:intergenic 50.0%
CGCGACTGCTATGAATGGTA+AGG - chr2.4:1819942-1819961 MS.gene89023:intron 50.0%
GCAAACCATCCAACAGCTCA+TGG + chr2.4:1819420-1819439 None:intergenic 50.0%
TGCGCATAACCATGAGCTGT+TGG - chr2.4:1819408-1819427 MS.gene89023:CDS 50.0%
TGGGCGACTGCTCATATCAA+AGG - chr2.4:1820237-1820256 MS.gene89023:intron 50.0%
TGTGTGAGGACTAAAGGGTG+TGG - chr2.4:1821038-1821057 MS.gene89023:CDS 50.0%
TTCGGAAGACCCAAGTTCTG+AGG - chr2.4:1819197-1819216 MS.gene89023:CDS 50.0%
! TCGGAAGACCCAAGTTCTGA+GGG - chr2.4:1819198-1819217 MS.gene89023:CDS 50.0%
!! CAAGTTCTGAGGGACCGTTT+GGG - chr2.4:1819208-1819227 MS.gene89023:CDS 50.0%
ACAGGGGATTGACCTTGGAG+AGG - chr2.4:1819281-1819300 MS.gene89023:CDS 55.0%
CCAAACGGTCCCTCAGAACT+TGG + chr2.4:1819210-1819229 None:intergenic 55.0%
CTTCCACCAGGCGTAGCAAT+TGG + chr2.4:1821071-1821090 None:intergenic 55.0%
GTCGAAGGATGTGGCTGAGA+GGG - chr2.4:1820866-1820885 MS.gene89023:CDS 55.0%
TAAACCCTCACACCGAGACG+TGG + chr2.4:1820286-1820305 None:intergenic 55.0%
TACTGGACCCTGCTTGTGTG+AGG - chr2.4:1821024-1821043 MS.gene89023:CDS 55.0%
TGTCGAAGGATGTGGCTGAG+AGG - chr2.4:1820865-1820884 MS.gene89023:CDS 55.0%
TTGCTACGCCTGGTGGAAGT+AGG - chr2.4:1821072-1821091 MS.gene89023:CDS 55.0%
!! CCAAGTTCTGAGGGACCGTT+TGG - chr2.4:1819207-1819226 MS.gene89023:CDS 55.0%
GGGGATTGACCTTGGAGAGG+CGG - chr2.4:1819284-1819303 MS.gene89023:CDS 60.0%
TGAGGACGCCTACTTCCACC+AGG + chr2.4:1821083-1821102 None:intergenic 60.0%
TTCTCGCTTCCGCCTCTCCA+AGG + chr2.4:1819296-1819315 None:intergenic 60.0%
! ACGAACCACGTCTCGGTGTG+AGG - chr2.4:1820278-1820297 MS.gene89023:intron 60.0%
! CGAACCACGTCTCGGTGTGA+GGG - chr2.4:1820279-1820298 MS.gene89023:intron 60.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 1818890 1821244 1818890 ID=MS.gene89023
chr2.4 mRNA 1818890 1821244 1818890 ID=MS.gene89023.t1;Parent=MS.gene89023
chr2.4 exon 1820442 1821244 1820442 ID=MS.gene89023.t1.exon1;Parent=MS.gene89023.t1
chr2.4 CDS 1820442 1821244 1820442 ID=cds.MS.gene89023.t1;Parent=MS.gene89023.t1
chr2.4 exon 1818890 1819589 1818890 ID=MS.gene89023.t1.exon2;Parent=MS.gene89023.t1
chr2.4 CDS 1818890 1819589 1818890 ID=cds.MS.gene89023.t1;Parent=MS.gene89023.t1
Gene Sequence

>MS.gene89023

ATGGAGGAGGTAACAGAAAGTTCAGCTGACAGTAATGACAATATTAACATCGAGGGAATGGACGAAGCTTCAGACGAAGGAGATAATATGGATGACAATGGGGCTAATGAGTGTGATGGAGATTTAGAAGCGAGTAGCGATTCATCCGAGTCTAATGGGAATGCTATAGTCATAAATGGCATCGGTGATATTTTAAAGATGGACATGAAAAACATTTCTGTAGATGTAGTTGAAAGGTTTGACTTTGCTGATTTAGATGTTGCATATAATTTCTACTCATGGTATGCAAGGATAAACGGATTTTCTGTTCGGAAGACCCAAGTTCTGAGGGACCGTTTGGGTAATGTTCTGCAGCAAACTTTTGTTTGTTCGCGGGAAGGTTTTAGAGAGAACAGGGGATTGACCTTGGAGAGGCGGAAGCGAGAACCCAGAAATGATACCAGGTGTGGATGTTCTGCAAAATGTCGTGTCCACATAGACATTGTTTCGAAGCGTTGGTATGTGACCGTATTTGAATTTGCGCATAACCATGAGCTGTTGGATGGTTTGCTATGTGGTCTTTTACCGGCACATAGAAAGCTAACACCAACTGATTTGATTGAGATTGAAAAGTATAAGAATGTTGGTGTAAGACCATATCAAACGTATGGTGCAATGGCTAATTCATCAGGTGGTTTTCACAAGTTAGGTTTTGTGAAAAAGGATTTGTATAATCAATCAGGAAGACAAAGGAAATTAGTTTCATCTGACTCTTGCGGTGCACTGAAGTATCTTCATATGCTTCGTACAAAAGACCCTTTAATGTATGTTGCACATACTGTAGACAAAGATAAGAGGCTTGAACGTCTTTTTTGGTGTGATGGAGAAAGTCGTATGAATTATGAAATTTTTGGTGATGTATTGGCCTTTGATGCAACCTATAGGAAAAACAAATACAATTGCCTGTTTGTTATTTTCTCCGGTGTTAATCATCATAACCAAACTGTTGTCTTTGCGACAGGTTTAGTTACGAGAGAGACGGAAGAGACATATGTTTGGTTGTTGGACCAATTCGCGACTGCTATGAATGGTAAGGCTCCTTTATCCGTAATAACTGACGGTGATGTTGCTATGAAAAATGCCATAAAGAGTATATTTCCAAAAGCACATCATAGATTATGTGCTTGGCATCTTCTACGGAATGCATCATCGAATGTTGGGATTACTGATTTCATGTCATATCTTAAAAGATGCATGTTGGGTGATATGGAAGTGAATAAGTTTGAAGAACTATGGGAAGAGATGGTTGAAAAATTTGGTCTGCAAGACAACAATTGGATCAATGAGATGTATGGAAAAAAAAGAATGTGGGCGACTGCTCATATCAAAGGAGAATTTTTTGCAGGTATACGAACCACGTCTCGGTGTGAGGGTTTACATAGTCACATAGGCCAGTTTGTGCATTCAAGAATTAACTTGACTGATTTTGTCCAGCAATTTCATAGGTGCCTTACATATTTTCGATTTCGAGAGATTGAGTCCGACTATCATTCCAACTATGGTCAACCCGTATTGCAAACTAGTTTGCGATTCATTGAGAGATCAGCAACGAACCAGTTTACAAGGGAGATTTTCTTCCTTTTTCGTAATGCTTTGAAAAAATCTTTGATGTTGAGAATTATTGAAATTCAAGAGATGTCCATGGGTTCCATCTACAAAGTTTCAAAGTACTGTGGAAATGGAAGTGTCTGGCACGTTACACATTGTGAAACTCCTGTAGATTTCAAATGTTCGTGTCTTAGGATGGAGTCGTTTGGACTGCCTTGCGATCATATTATTGCTGTTTTGATGTATCTGGATTTTGATCACCTTCCCAAGAATCTAGTTTTACACAGGTGGACAAAGTTCGCTACAGATACAGTTCGAGCAAGATACACAAATGGTTCACACTATTGGGATTCTCAAATTGCTTCAAGGTATGCGGGAATTATTCAGTTGTCGAAGGATGTGGCTGAGAGGGTTTATCAAGATGTAGAAGAGTACAACCACATTGTAGATTTTCTTAGTGCTGAACTTTTGAGGTTGAAGAATAAAACAACAGCTGTTGGTATTGATGCTAGCACTGGGGTACAAAATGTAGTCGTCGACGAAAGTTTACTGGACCCTGCTTGTGTGAGGACTAAAGGGTGTGGATCTAATCCAATTGCTACGCCTGGTGGAAGTAGGCGTCCTCAATCATGTAGTCAGTGTGGTGTGATAGGACACAATAAACGATCATGTCCTAAAGGAGTCAACAACGGTGAAAATGCATGCACTTCTAGTGCAACAATTTATGAAATTAATGAAGATGATGAAGAAGGAGATCTTGAGCTGTGA
Protein sequence

>MS.gene89023.t1

MEEVTESSADSNDNINIEGMDEASDEGDNMDDNGANECDGDLEASSDSSESNGNAIVINGIGDILKMDMKNISVDVVERFDFADLDVAYNFYSWYARINGFSVRKTQVLRDRLGNVLQQTFVCSREGFRENRGLTLERRKREPRNDTRCGCSAKCRVHIDIVSKRWYVTVFEFAHNHELLDGLLCGLLPAHRKLTPTDLIEIEKYKNVGVRPYQTYGAMANSSGGFHKLGFVKKDLYNQSGRQRKLVSSDSCGALKYLHMLRTKDPLIIIEIQEMSMGSIYKVSKYCGNGSVWHVTHCETPVDFKCSCLRMESFGLPCDHIIAVLMYLDFDHLPKNLVLHRWTKFATDTVRARYTNGSHYWDSQIASRYAGIIQLSKDVAERVYQDVEEYNHIVDFLSAELLRLKNKTTAVGIDASTGVQNVVVDESLLDPACVRTKGCGSNPIATPGGSRRPQSCSQCGVIGHNKRSCPKGVNNGENACTSSATIYEINEDDEEGDLEL