AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene89183

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene89183.t1 MTR_5g065200 75.878 854 153 9 22 860 19 834 0.0 1269
MS.gene89183.t1 MTR_1g114150 74.428 743 162 6 123 860 331 1050 0.0 1151
MS.gene89183.t1 MTR_1g114130 72.995 748 184 6 116 862 310 1040 0.0 1124
MS.gene89183.t1 MTR_1g069565 62.942 904 266 13 3 860 1 881 0.0 1077
MS.gene89183.t1 MTR_1g114070 68.207 736 206 4 126 860 216 924 0.0 1034
MS.gene89183.t1 MTR_1g069565 58.793 961 270 16 3 860 1 938 0.0 1031
MS.gene89183.t1 MTR_1g114150 71.835 632 150 6 123 749 331 939 0.0 939
MS.gene89183.t1 MTR_5g047620 66.317 668 196 11 207 860 145 797 0.0 874
MS.gene89183.t1 MTR_0020s0100 61.598 651 198 11 203 843 83 691 0.0 770
MS.gene89183.t1 MTR_7g117445 45.839 805 345 15 131 859 136 925 0.0 667
MS.gene89183.t1 MTR_7g117445 45.839 805 345 15 131 859 136 925 0.0 667
MS.gene89183.t1 MTR_7g117445 45.839 805 345 15 131 859 136 925 0.0 667
MS.gene89183.t1 MTR_4g132540 44.473 778 349 15 110 861 147 867 0.0 628
MS.gene89183.t1 MTR_2g024270 46.866 734 322 13 129 858 148 817 0.0 606
MS.gene89183.t1 MTR_7g117445 44.430 745 323 15 131 799 136 865 0.0 590
MS.gene89183.t1 MTR_2g024140 45.516 736 319 13 128 858 27 685 0.0 585
MS.gene89183.t1 MTR_1g083120 36.779 745 407 14 135 862 198 895 4.35e-152 471
MS.gene89183.t1 MTR_1g083120 36.779 745 407 14 135 862 215 912 9.01e-152 470
MS.gene89183.t1 MTR_1g083120 36.404 684 371 14 135 801 198 834 1.41e-133 420
MS.gene89183.t1 MTR_1g083120 36.404 684 371 14 135 801 198 834 1.66e-133 420
MS.gene89183.t1 MTR_2g011630 34.675 770 433 19 135 857 207 953 6.39e-131 416
MS.gene89183.t1 MTR_1g008060 43.123 538 266 11 129 661 864 1366 8.17e-127 418
MS.gene89183.t1 MTR_1g008060 72.059 136 38 0 714 849 1551 1686 1.20e-57 216
MS.gene89183.t1 MTR_1g008060 43.123 538 266 11 129 661 855 1357 1.17e-126 418
MS.gene89183.t1 MTR_1g008060 72.059 136 38 0 714 849 1542 1677 1.38e-57 216
MS.gene89183.t1 MTR_2g011630 35.044 682 383 16 219 857 45 709 8.09e-117 372
MS.gene89183.t1 MTR_2g011630 35.044 682 383 16 219 857 77 741 1.35e-116 372
MS.gene89183.t1 MTR_8g062940 51.381 362 143 4 135 496 33 361 4.00e-113 350
MS.gene89183.t1 MTR_2g024240 54.019 311 107 3 513 823 114 388 2.91e-105 330
MS.gene89183.t1 MTR_4g091520 36.121 562 286 15 359 857 727 1278 1.05e-93 321
MS.gene89183.t1 MTR_4g091520 36.905 168 99 3 135 297 260 425 2.87e-26 116
MS.gene89183.t1 MTR_0295s0020 58.152 184 70 1 486 669 1 177 6.91e-62 207
MS.gene89183.t1 MTR_4g091520 32.632 475 250 14 359 773 657 1121 6.82e-59 219
MS.gene89183.t1 MTR_4g091520 36.905 168 99 3 135 297 260 425 2.10e-26 117
MS.gene89183.t1 MTR_4g091520 32.632 475 250 14 359 773 692 1156 8.01e-59 219
MS.gene89183.t1 MTR_4g091520 36.905 168 99 3 135 297 260 425 1.88e-26 117
MS.gene89183.t1 MTR_4g091520 32.632 475 250 14 359 773 727 1191 9.95e-59 218
MS.gene89183.t1 MTR_4g091520 36.905 168 99 3 135 297 260 425 2.17e-26 117
MS.gene89183.t1 MTR_8g465700 51.795 195 87 2 448 642 4 191 6.82e-58 199
MS.gene89183.t1 MTR_8g013920 60.256 156 43 3 538 693 40 176 1.97e-42 153
MS.gene89183.t1 MTR_8g465720 75.000 88 21 1 714 801 53 139 1.47e-40 147
MS.gene89183.t1 MTR_3g080750 57.377 61 20 1 624 678 85 145 5.20e-13 67.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene89183.t1 AT1G11950 48.098 736 326 10 131 862 189 872 0.0 674
MS.gene89183.t1 AT1G11950 48.098 736 326 10 131 862 189 872 0.0 674
MS.gene89183.t1 AT3G07610 45.207 772 340 8 134 857 154 890 0.0 661
MS.gene89183.t1 AT1G62310 46.433 743 343 13 110 842 181 878 0.0 655
MS.gene89183.t1 AT3G07610 43.995 791 344 9 134 857 154 912 0.0 641
MS.gene89183.t1 AT4G00990 42.529 783 364 17 135 861 80 832 0.0 590
MS.gene89183.t1 AT1G11950 46.015 665 303 10 131 791 189 801 0.0 568
MS.gene89183.t1 AT4G00990 41.427 729 341 17 135 807 80 778 1.95e-174 524
MS.gene89183.t1 AT4G00990 41.748 721 334 17 135 799 80 770 1.37e-173 521
MS.gene89183.t1 AT3G07610 42.125 546 259 6 134 631 154 690 3.13e-136 424
MS.gene89183.t1 AT1G09060 31.788 755 438 17 128 862 197 894 6.96e-116 375
MS.gene89183.t1 AT1G09060 31.788 755 438 17 128 862 197 894 6.96e-116 375
MS.gene89183.t1 AT1G09060 31.788 755 438 17 128 862 211 908 7.42e-116 375
MS.gene89183.t1 AT1G09060 31.788 755 438 17 128 862 211 908 7.42e-116 375
MS.gene89183.t1 AT4G21430 29.799 745 453 14 129 857 221 911 1.16e-93 315

Find 191 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCACATTTATTCTTAGATTT+TGG 0.128373 5.2:+66766802 None:intergenic
AGTGCTCTCTGAAGCATTTA+TGG 0.163893 5.2:+66765128 None:intergenic
GGATTTGTATATTCTTTAAA+TGG 0.213495 5.2:+66765926 None:intergenic
GATCTTTATCACGAGGATTT+AGG 0.216600 5.2:-66766544 MS.gene89183:CDS
TCATATTGCTAAAGCAAAAT+TGG 0.216686 5.2:-66765259 MS.gene89183:CDS
GGTTGGTGGTTCTACGTTCT+TGG 0.246061 5.2:+66768237 None:intergenic
CTTCGTTGTGGCCAACTTCT+AGG 0.252701 5.2:-66766997 MS.gene89183:CDS
CTATCCTTCATCGCTATTCT+AGG 0.275882 5.2:-66768297 MS.gene89183:CDS
AGTACATTGCTGACAATTAC+AGG 0.278275 5.2:+66766490 None:intergenic
TGTCATTTCTTTGACATTGA+TGG 0.280482 5.2:+66767877 None:intergenic
TGTCTTGCTTAAGGTGCCTT+TGG 0.287167 5.2:+66765202 None:intergenic
ACTAAGTCAGAGATGTAATT+TGG 0.287365 5.2:+66766745 None:intergenic
GACCAAGCACAAGCCACTTT+TGG 0.295143 5.2:-66766401 MS.gene89183:CDS
GATGATGATGGCCAATCTTC+TGG 0.296228 5.2:-66765105 MS.gene89183:CDS
CAGCTCTTTGCGTAGATTCC+AGG 0.304385 5.2:-66766597 MS.gene89183:CDS
TACCTCCAATTTGTCCTCTA+TGG 0.305460 5.2:+66762150 None:intergenic
TCTGGCTTTAGAACATTATC+AGG 0.310569 5.2:+66765869 None:intergenic
AGTACGCGCGCTCATCATTA+TGG 0.311143 5.2:+66767199 None:intergenic
GCTCTTGTTTGAAGCCTGTT+AGG 0.324611 5.2:-66766654 MS.gene89183:CDS
GTCTTGCTTAAGGTGCCTTT+GGG 0.327340 5.2:+66765203 None:intergenic
TCTATGGACCTGTGATTTAC+TGG 0.329135 5.2:+66762166 None:intergenic
GCTCCCTCTTGTCTTGCTTA+AGG 0.329503 5.2:+66765193 None:intergenic
ATTCTTTAGATGGTGCTCTC+TGG 0.331445 5.2:-66765020 MS.gene89183:CDS
AGCATGAGGTGCTTTGTATC+TGG 0.334250 5.2:+66766897 None:intergenic
AATGTGCACCAATTCTTTAC+CGG 0.348314 5.2:-66766073 MS.gene89183:CDS
CAAGAAAATGGTTTGTTGGT+TGG 0.352054 5.2:-66765045 MS.gene89183:CDS
CGTCCATTGCTCTCCTTTAG+AGG 0.359183 5.2:-66764926 MS.gene89183:CDS
CAACACAATTCGTCGTAATA+AGG 0.364645 5.2:-66768029 MS.gene89183:intron
CTTTCCTCAAATAAATTAGA+AGG 0.368022 5.2:+66765986 None:intergenic
ATGCCGGACGGTTAGATAAA+AGG 0.368269 5.2:+66763653 None:intergenic
GCGACGATGAAACAAGAAAA+TGG 0.376184 5.2:-66765057 MS.gene89183:CDS
TTGGTTGGAGATTCTTTAGA+TGG 0.389380 5.2:-66765030 MS.gene89183:CDS
ATACGTACATGGTCAAAATC+TGG 0.391419 5.2:-66766859 MS.gene89183:CDS
AGAGGACGTACCCGCATTGC+AGG 0.392255 5.2:-66764983 MS.gene89183:CDS
ATTTATGGAAGAAGACAAAT+TGG 0.392384 5.2:+66765143 None:intergenic
AAGATAAACTCAAAATCAAA+TGG 0.394363 5.2:+66766964 None:intergenic
GCCTCCTTCTAATTTATTTG+AGG 0.400880 5.2:-66765990 MS.gene89183:CDS
ATGTCATCTCTGTTCCTAAC+AGG 0.405147 5.2:+66766640 None:intergenic
AGGCCAATCCTGTTTATCCT+TGG 0.405342 5.2:+66766036 None:intergenic
TCTACCACATTTAATACGAT+TGG 0.415607 5.2:-66767447 MS.gene89183:CDS
GGCCAATCCTGTTTATCCTT+GGG 0.421151 5.2:+66766037 None:intergenic
GGAGCTACATGGTGATAATC+AGG 0.421783 5.2:-66765175 MS.gene89183:CDS
AATGTACTTGAATGTACATC+TGG 0.421952 5.2:-66766475 MS.gene89183:CDS
ATATAAAATACCTTGTATCT+TGG 0.422375 5.2:+66767391 None:intergenic
TTCCACCATGCAGATAATCT+TGG 0.430174 5.2:+66766935 None:intergenic
GAGTATTGAAATTGAAAGAT+TGG 0.430433 5.2:-66766012 MS.gene89183:CDS
CCTTCAAATTTCTGACTTGG+TGG 0.430574 5.2:+66763440 None:intergenic
AGAAGCATTTCAAAGAGTTC+AGG 0.434223 5.2:-66764951 MS.gene89183:CDS
TTGTGTACCCGGTAAAGAAT+TGG 0.436382 5.2:+66766065 None:intergenic
TTCAGATATTCCTGCAATGC+GGG 0.438768 5.2:+66764973 None:intergenic
GAAAATGATTATATACACTA+TGG 0.439229 5.2:-66761538 MS.gene89183:CDS
AAATTGTCCCAAGGATAAAC+AGG 0.439965 5.2:-66766044 MS.gene89183:CDS
TTCTTTAGATGGTGCTCTCT+GGG 0.441915 5.2:-66765019 MS.gene89183:CDS
AAGAGCTTTGAGCATATACT+TGG 0.442351 5.2:+66767469 None:intergenic
CGTTAAACGCAACCGTAAAA+CGG 0.451202 5.2:-66768212 MS.gene89183:CDS
GAAATATGCTTCTCAGTTCA+AGG 0.451338 5.2:+66766770 None:intergenic
AATGTACATCTGGTTTAAGC+TGG 0.451500 5.2:-66766465 MS.gene89183:CDS
TTCAAATTTCTGACTTGGTG+GGG 0.453619 5.2:+66763442 None:intergenic
CTTCAAATTTCTGACTTGGT+GGG 0.454550 5.2:+66763441 None:intergenic
AACAAAATCAAGTGCAACCT+TGG 0.456526 5.2:+66762243 None:intergenic
CCACCAAGTCAGAAATTTGA+AGG 0.456844 5.2:-66763440 MS.gene89183:intron
CAGGTCCATAGAGGACAAAT+TGG 0.466017 5.2:-66762155 MS.gene89183:CDS
GAAACAAGAAAATGGTTTGT+TGG 0.467660 5.2:-66765049 MS.gene89183:CDS
AATTCAGAAATGGCGTTGGC+GGG 0.468398 5.2:-66768394 None:intergenic
AGGCGCTTGAATGCTTAGAT+TGG 0.470973 5.2:-66766372 MS.gene89183:CDS
ATAATGATGAGCGCGCGTAC+TGG 0.472042 5.2:-66767197 MS.gene89183:intron
CTCAACCTGATGATCATTGT+TGG 0.473165 5.2:+66767496 None:intergenic
TCTAAGAATAAATGTGATCA+TGG 0.477946 5.2:-66766796 MS.gene89183:CDS
TCTTCCTAGAATAGCGATGA+AGG 0.478322 5.2:+66768293 None:intergenic
TGACCTCTGAGCCAGAAGAT+TGG 0.482683 5.2:+66765094 None:intergenic
CGATACCAACAATGATCATC+AGG 0.484087 5.2:-66767501 MS.gene89183:CDS
AAAAGAACTTGCAGATTCAA+TGG 0.485093 5.2:-66766713 MS.gene89183:CDS
TGCGGAATTCCTCTGTCAAA+CGG 0.487229 5.2:+66762194 None:intergenic
TGTGATTTACTGGAAGTTTG+CGG 0.491065 5.2:+66762176 None:intergenic
ATCTTCTGGCTCAGAGGTCA+AGG 0.494381 5.2:-66765091 MS.gene89183:CDS
TTCAATGGCACTGGAGCAGA+GGG 0.494515 5.2:-66766516 MS.gene89183:CDS
TTACCTTCAAATTTCTGACT+TGG 0.497862 5.2:+66763437 None:intergenic
GAATTCAGAAATGGCGTTGG+CGG 0.503051 5.2:-66768395 None:intergenic
GTGTATGCTGTATTAACAAA+TGG 0.503342 5.2:-66767809 MS.gene89183:intron
TCAAATTTCTGACTTGGTGG+GGG 0.505542 5.2:+66763443 None:intergenic
GTCAAAGAAATGACAAAGGA+AGG 0.505650 5.2:-66767869 MS.gene89183:CDS
CGGTTGCGTTTAACGGTGGT+TGG 0.507184 5.2:+66768220 None:intergenic
ATCCAAAAGTGGCTTGTGCT+TGG 0.509797 5.2:+66766399 None:intergenic
TGTATATTCTTTAAATGGCA+AGG 0.512473 5.2:+66765931 None:intergenic
AGCTCTTTGCGTAGATTCCA+GGG 0.513640 5.2:-66766596 MS.gene89183:CDS
GCAAAGGGTGTTTGAGATCA+CGG 0.514544 5.2:-66767645 MS.gene89183:CDS
CATGCAGATAATCTTGGCCT+CGG 0.514765 5.2:+66766941 None:intergenic
TGATAAAGATCTACTGCCCT+AGG 0.515253 5.2:+66766556 None:intergenic
AGATAAACTCAAAATCAAAT+GGG 0.515679 5.2:+66766965 None:intergenic
CCATGTACGTATCTCAAGTT+CGG 0.518603 5.2:+66766870 None:intergenic
TGTATGTAGGAATTGAGCCT+TGG 0.523387 5.2:-66763513 MS.gene89183:intron
CGCAACCGTAAAACGGAAGA+AGG 0.528137 5.2:-66768205 MS.gene89183:CDS
CTAGAATAGCGATGAAGGAT+AGG 0.533251 5.2:+66768298 None:intergenic
CTTGGGACAATTTGTGTACC+CGG 0.535581 5.2:+66766054 None:intergenic
ACTAACTTTACCTCCTCTAA+AGG 0.536444 5.2:+66764913 None:intergenic
TTAGATAAAAGGTCTGATCA+TGG 0.536799 5.2:+66763664 None:intergenic
TTGCGTTTAACGGTGGTTGG+TGG 0.543542 5.2:+66768223 None:intergenic
ACAAACACATCTTATCATGC+TGG 0.544179 5.2:-66768124 MS.gene89183:CDS
GAGATAGAAGCCAAGATACA+AGG 0.546758 5.2:-66767401 MS.gene89183:intron
AGCGCCTTAACATCCAAAAG+TGG 0.548428 5.2:+66766388 None:intergenic
ATGTGCACCAATTCTTTACC+GGG 0.551064 5.2:-66766072 MS.gene89183:CDS
TAAAAGGTCTGATCATGGAT+GGG 0.555220 5.2:+66763669 None:intergenic
AGACATATCACAATGGAGCT+TGG 0.555405 5.2:+66765781 None:intergenic
TTGCAATTACCACAACATAC+AGG 0.555718 5.2:+66767670 None:intergenic
GGTCTGCACCACCTAGAAGT+TGG 0.555830 5.2:+66766986 None:intergenic
ATAAAAGGTCTGATCATGGA+TGG 0.557609 5.2:+66763668 None:intergenic
TCTTTCAATTTCAATACTCG+AGG 0.562439 5.2:+66766016 None:intergenic
AGGTTGAGAGCACCTTTGAA+AGG 0.563537 5.2:+66765905 None:intergenic
TACGTACATGGTCAAAATCT+GGG 0.563636 5.2:-66766858 MS.gene89183:CDS
GTGGTAATTGCAATTGCAAA+GGG 0.564159 5.2:-66767660 MS.gene89183:CDS
TGGTCTCTCAGTCGCAGCCA+CGG 0.564180 5.2:+66768321 None:intergenic
CAACAATGATCATCAGGTTG+AGG 0.564275 5.2:-66767495 MS.gene89183:CDS
ACGCAGAACAAACGCGACGA+AGG 0.566903 5.2:-66768269 MS.gene89183:CDS
GTCCCAAGGATAAACAGGAT+TGG 0.567361 5.2:-66766039 MS.gene89183:CDS
GAATATACAAATCCTTTCAA+AGG 0.567529 5.2:-66765917 MS.gene89183:CDS
AACGAAGCTCGCGGCAACAG+AGG 0.568999 5.2:+66767013 None:intergenic
AGATTATCTGCATGGTGGAA+AGG 0.569015 5.2:-66766932 MS.gene89183:CDS
TTAATTGCATCAATACTCGC+AGG 0.570155 5.2:+66765234 None:intergenic
ATGTACATCTGGTTTAAGCT+GGG 0.572127 5.2:-66766464 MS.gene89183:CDS
ATTTGATCTTCTTCCAAGTG+AGG 0.572865 5.2:+66767706 None:intergenic
ATGACATACACAATAACTCA+AGG 0.573104 5.2:-66766624 MS.gene89183:CDS
TTTCAATGGCACTGGAGCAG+AGG 0.573700 5.2:-66766517 MS.gene89183:CDS
TTTGAATTCAGAAATGGCGT+TGG 0.581594 5.2:-66768398 None:intergenic
GTTGTTAAGGTATCCTCACT+TGG 0.581865 5.2:-66767719 MS.gene89183:intron
GCTGGGAGCCGCTTGTCATG+TGG 0.582104 5.2:-66766447 MS.gene89183:CDS
TCAACAACAAATGTCTGAGA+AGG 0.585137 5.2:-66767423 MS.gene89183:CDS
GTCCATAGAGGACAAATTGG+AGG 0.587047 5.2:-66762152 MS.gene89183:intron
CAAAACATTAGAGAAAGCAT+TGG 0.588335 5.2:-66761505 MS.gene89183:CDS
AGTAAATCACAGGTCCATAG+AGG 0.591019 5.2:-66762164 MS.gene89183:CDS
AGCTTCTGAACAAAAGTCCA+AGG 0.591626 5.2:+66763496 None:intergenic
CGAGGCCAAGATTATCTGCA+TGG 0.592075 5.2:-66766940 MS.gene89183:CDS
TATCAGGCAGCTTCACAGCA+AGG 0.593858 5.2:+66765885 None:intergenic
CAAGACAAGAGGGAGCTACA+TGG 0.594046 5.2:-66765186 MS.gene89183:CDS
ACGACGGCGTCATCGTTGAA+CGG 0.597980 5.2:-66768355 MS.gene89183:CDS
GCAAACTTCCAGTAAATCAC+AGG 0.600882 5.2:-66762174 MS.gene89183:CDS
TGATCCAATCGTATTAAATG+TGG 0.601526 5.2:+66767443 None:intergenic
AAAAGGTCTGATCATGGATG+GGG 0.603357 5.2:+66763670 None:intergenic
GCACCTTAAGCAAGACAAGA+GGG 0.606408 5.2:-66765196 MS.gene89183:CDS
TCCTCAAATAAATTAGAAGG+AGG 0.607054 5.2:+66765989 None:intergenic
TCTGCATGGTGGAAAGGCGG+AGG 0.608026 5.2:-66766926 MS.gene89183:CDS
TCGAACAACAACAGCAGCAA+CGG 0.610845 5.2:+66768168 None:intergenic
AAAGATCTACTGCCCTAGGA+TGG 0.611057 5.2:+66766560 None:intergenic
TTGTTGTTCGAAAGAGTGAA+CGG 0.611861 5.2:-66768156 MS.gene89183:CDS
TAATGTTCTAAAGCCAGACA+TGG 0.612070 5.2:-66765864 MS.gene89183:CDS
TCAGAGAGCACTGATGATGA+TGG 0.614655 5.2:-66765117 MS.gene89183:CDS
TGTTGCCGCGAGCTTCGTTG+TGG 0.615230 5.2:-66767009 MS.gene89183:CDS
AGATACAAAGCACCTCATGC+TGG 0.616281 5.2:-66766895 MS.gene89183:CDS
AATGCACGCCACATGACAAG+CGG 0.617629 5.2:+66766439 None:intergenic
TTTACGGTTGCGTTTAACGG+TGG 0.618613 5.2:+66768216 None:intergenic
TCAAAGAAATGACAAAGGAA+GGG 0.618886 5.2:-66767868 MS.gene89183:CDS
AATTTAACAGTGGATTGAAG+AGG 0.619121 5.2:-66767913 MS.gene89183:intron
CAGAACAAACGCGACGAAGG+AGG 0.619183 5.2:-66768266 MS.gene89183:CDS
GGCACCTTAAGCAAGACAAG+AGG 0.620603 5.2:-66765197 MS.gene89183:CDS
GGGTGGCATGCAGATAGTAA+TGG 0.625312 5.2:-66766838 MS.gene89183:CDS
CAATTAAGAAATTGACCCAA+AGG 0.625816 5.2:-66765218 MS.gene89183:CDS
TGGCCAATCTTCTGGCTCAG+AGG 0.629159 5.2:-66765097 MS.gene89183:CDS
AATGGAACTTCAAGATGCAG+AGG 0.629339 5.2:-66766695 MS.gene89183:CDS
GAACGGTTCATGCGACGCCG+TGG 0.631294 5.2:-66768338 MS.gene89183:CDS
CCGAACTTGAGATACGTACA+TGG 0.632394 5.2:-66766870 MS.gene89183:CDS
CCATTGCTCTCCTTTAGAGG+AGG 0.635145 5.2:-66764923 MS.gene89183:intron
CTACATGGTGATAATCAGGA+TGG 0.636072 5.2:-66765171 MS.gene89183:CDS
CTTCAGATATTCCTGCAATG+CGG 0.636107 5.2:+66764972 None:intergenic
GGCAGTAGATCTTTATCACG+AGG 0.641811 5.2:-66766551 MS.gene89183:CDS
TATATACACTATGGTTGATG+TGG 0.641942 5.2:-66761529 MS.gene89183:CDS
TGTGGTAATTGCAATTGCAA+AGG 0.644805 5.2:-66767661 MS.gene89183:CDS
GAATAGCGATGAAGGATAGG+TGG 0.645201 5.2:+66768301 None:intergenic
CCTCCTCTAAAGGAGAGCAA+TGG 0.645248 5.2:+66764923 None:intergenic
CGTTGTGGCCAACTTCTAGG+TGG 0.649865 5.2:-66766994 MS.gene89183:CDS
GAGGCATGTCCTGTATGTTG+TGG 0.650351 5.2:-66767679 MS.gene89183:CDS
CGGGTACACAAATTGTCCCA+AGG 0.655726 5.2:-66766053 MS.gene89183:CDS
TTTGTTTCTCCTGAAAACGT+TGG 0.656335 5.2:-66762226 MS.gene89183:CDS
TTATCTGCATGGTGGAAAGG+CGG 0.658125 5.2:-66766929 MS.gene89183:CDS
GTTGGCCACAACGAAGCTCG+CGG 0.661562 5.2:+66767004 None:intergenic
CGCGGCAACAGAGGACACAA+AGG 0.661878 5.2:+66767022 None:intergenic
TTAGATGCACCACATGCCAA+CGG 0.661969 5.2:-66767842 MS.gene89183:CDS
TGACATACACAATAACTCAA+GGG 0.663436 5.2:-66766623 MS.gene89183:CDS
TGAGTGCTTCCGTTTGACAG+AGG 0.665002 5.2:-66762203 MS.gene89183:CDS
TTCTGGCTCAGAGGTCAAGG+AGG 0.666246 5.2:-66765088 MS.gene89183:CDS
AATGTTCTAAAGCCAGACAT+GGG 0.666543 5.2:-66765863 MS.gene89183:CDS
GGAAGAAGATCAAATTGCTG+AGG 0.669254 5.2:-66767698 MS.gene89183:CDS
TCAAGTTCGGAACCAGCATG+AGG 0.678381 5.2:+66766883 None:intergenic
TGGCAAGGAAGATATGAACT+CGG 0.681914 5.2:+66765946 None:intergenic
GCTCTTTGCGTAGATTCCAG+GGG 0.682462 5.2:-66766595 MS.gene89183:CDS
CAATGTCAAAGAAATGACAA+AGG 0.691997 5.2:-66767873 MS.gene89183:CDS
GGCCAAGATTATCTGCATGG+TGG 0.693766 5.2:-66766937 MS.gene89183:CDS
TGAATGCTTAGATTGGTGCG+AGG 0.693830 5.2:-66766365 MS.gene89183:intron
ATGAACTCGGCACAATGACG+AGG 0.695465 5.2:+66765959 None:intergenic
AGAAGACGACACATTGACGA+CGG 0.696403 5.2:-66768371 MS.gene89183:CDS
CTGCATCAGACATATCACAA+TGG 0.714139 5.2:+66765774 None:intergenic
GTACATGGTCAAAATCTGGG+TGG 0.756530 5.2:-66766855 MS.gene89183:CDS
TCAATGGCACTGGAGCAGAG+GGG 0.763341 5.2:-66766515 MS.gene89183:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr5.2 gene 66761500 66768407 66761500 ID=MS.gene89183
chr5.2 mRNA 66761500 66768407 66761500 ID=MS.gene89183.t1;Parent=MS.gene89183
chr5.2 exon 66768030 66768407 66768030 ID=MS.gene89183.t1.exon1;Parent=MS.gene89183.t1
chr5.2 CDS 66768030 66768407 66768030 ID=cds.MS.gene89183.t1;Parent=MS.gene89183.t1
chr5.2 exon 66767810 66767925 66767810 ID=MS.gene89183.t1.exon2;Parent=MS.gene89183.t1
chr5.2 CDS 66767810 66767925 66767810 ID=cds.MS.gene89183.t1;Parent=MS.gene89183.t1
chr5.2 exon 66767633 66767732 66767633 ID=MS.gene89183.t1.exon3;Parent=MS.gene89183.t1
chr5.2 CDS 66767633 66767732 66767633 ID=cds.MS.gene89183.t1;Parent=MS.gene89183.t1
chr5.2 exon 66767402 66767546 66767402 ID=MS.gene89183.t1.exon4;Parent=MS.gene89183.t1
chr5.2 CDS 66767402 66767546 66767402 ID=cds.MS.gene89183.t1;Parent=MS.gene89183.t1
chr5.2 exon 66767198 66767261 66767198 ID=MS.gene89183.t1.exon5;Parent=MS.gene89183.t1
chr5.2 CDS 66767198 66767261 66767198 ID=cds.MS.gene89183.t1;Parent=MS.gene89183.t1
chr5.2 exon 66766366 66767104 66766366 ID=MS.gene89183.t1.exon6;Parent=MS.gene89183.t1
chr5.2 CDS 66766366 66767104 66766366 ID=cds.MS.gene89183.t1;Parent=MS.gene89183.t1
chr5.2 exon 66765775 66766104 66765775 ID=MS.gene89183.t1.exon7;Parent=MS.gene89183.t1
chr5.2 CDS 66765775 66766104 66765775 ID=cds.MS.gene89183.t1;Parent=MS.gene89183.t1
chr5.2 exon 66764924 66765295 66764924 ID=MS.gene89183.t1.exon8;Parent=MS.gene89183.t1
chr5.2 CDS 66764924 66765295 66764924 ID=cds.MS.gene89183.t1;Parent=MS.gene89183.t1
chr5.2 exon 66763628 66763700 66763628 ID=MS.gene89183.t1.exon9;Parent=MS.gene89183.t1
chr5.2 CDS 66763628 66763700 66763628 ID=cds.MS.gene89183.t1;Parent=MS.gene89183.t1
chr5.2 exon 66763441 66763526 66763441 ID=MS.gene89183.t1.exon10;Parent=MS.gene89183.t1
chr5.2 CDS 66763441 66763526 66763441 ID=cds.MS.gene89183.t1;Parent=MS.gene89183.t1
chr5.2 exon 66762153 66762272 66762153 ID=MS.gene89183.t1.exon11;Parent=MS.gene89183.t1
chr5.2 CDS 66762153 66762272 66762153 ID=cds.MS.gene89183.t1;Parent=MS.gene89183.t1
chr5.2 exon 66761500 66761565 66761500 ID=MS.gene89183.t1.exon12;Parent=MS.gene89183.t1
chr5.2 CDS 66761500 66761565 66761500 ID=cds.MS.gene89183.t1;Parent=MS.gene89183.t1
Gene Sequence

>MS.gene89183

ATGGCGTTGGCGGGAGAAGACGACACATTGACGACGGCGTCATCGTTGAACGGTTCATGCGACGCCGTGGCTGCGACTGAGAGACCACCTATCCTTCATCGCTATTCTAGGAAGAAACGCAGAACAAACGCGACGAAGGAGGATTTAACCAAGAACGTAGAACCACCAACCACCGTTAAACGCAACCGTAAAACGGAAGAAGGTGCTTCTTCTACTACCGTTGCTGCTGTTGTTGTTCGAAAGAGTGAACGGATAAGAAAAACAAACACATCTTATCATGCTGGTTTAGAGCTTCAAGAAAAACCTAAAAAAGGAAAACAGAGTCATCAAAATCATACGAAAGCTGAAAAAATCAACAACACAATTCGTCGTAATAAGGTCACAACGTCTTCTCTTCTTCTTCTAATGATTACTACTATTATTATTGTTACATTCCTTTGTGGTTCTGTAATTTACTAAATCTGATTCAACAAATTTAACAGTGGATTGAAGAGGAGTCTTTAATGTGCCATCAATGTCAAAGAAATGACAAAGGAAGGGTTGTTAGATGCACCACATGCCAACGGAAAAGATTTTGTGTATGCTGTATTAACAAATGGTATGTATCTATATTTCTACAACTTCTTCATTTGCCAATGTAATGTATCATATATATATATTGTTTTTGTTGTTAAGGTATCCTCACTTGGAAGAAGATCAAATTGCTGAGGCATGTCCTGTATGTTGTGGTAATTGCAATTGCAAAGGGTGTTTGAGATCACGGAAACTTATCGATGTATGTCTCTAACTTCTTCGACCCCTTTTACGTACATTTTCTCTATTCATTCAACATTTTTAATCTTATTATCTTATACATTACAGTCAATTAAACAAAAAGAGGACGACGATACCAACAATGATCATCAGGTTGAGGTCTCCAAGTATATGCTCAAAGCTCTTCTACCACATTTAATACGATTGGATCAACAACAAATGTCTGAGAAGGAGATAGAAGCCAAGATACAAGGTATTTTATATACACCTTTCTTTCTTTTCAATTACTTACATACTAATTCATTTCCCACATGTGCTTTTTCATGGTCGATCATTTTTAGTTTCATATATTTCCTCTTTTTTTTTAATTTGCTTTTGGGATCAACATTGTAGGACGCTCACTCTCTGAGCTAAAAATTAAAGTATCAGTTCTCCATAATGATGAGCGCGCGTACTGGTAGGGAATTATTTCGTATATAAATTGGATTTTTACGTAGTTAGTTAGTTATTCCAATCCTATGTTGTTATTAATGACTCATTACTTTTGTAGTGACAACTGCAAAACATCAATATTTGACTACCACAGAAGATGCACAAAATGCTCTTTTGACCTTTGTGTCCTCTGTTGCCGCGAGCTTCGTTGTGGCCAACTTCTAGGTGGTGCAGACCCATTTGATTTTGAGTTTATCTTCCGAGGCCAAGATTATCTGCATGGTGGAAAGGCGGAGGAAGAAACCAGATACAAAGCACCTCATGCTGGTTCCGAACTTGAGATACGTACATGGTCAAAATCTGGGTGGCATGCAGATAGTAATGGCAATATTCCCTGTCCAAAATCTAAGAATAAATGTGATCATGGTTACCTTGAACTGAGAAGCATATTTCAGCCAAATTACATCTCTGACTTAGTATGTAAAGCAAAAGAACTTGCAGATTCAATGGAACTTCAAGATGCAGAGGAGACTCTTGATAATAGCTGCTCTTGTTTGAAGCCTGTTAGGAACAGAGATGACATACACAATAACTCAAGGGAAGCAGCTCTTTGCGTAGATTCCAGGGGCAATTTTTTGTACCATCCTAGGGCAGTAGATCTTTATCACGAGGATTTAGGACATTTTCAATGGCACTGGAGCAGAGGGGAGCCTGTAATTGTCAGCAATGTACTTGAATGTACATCTGGTTTAAGCTGGGAGCCGCTTGTCATGTGGCGTGCATTTCGTCAGAAAAGCAAGACCAAGCACAAGCCACTTTTGGATGTTAAGGCGCTTGAATGCTTAGATTGGTGCGAGGTTTGTTCGATTTCTAAACCTTGTACATGAATTTTGTTCGATTTCTTGATCTTTGAGCTTCTCATATGGTTTCTTGCACTCTAGTGTCCCTTGCATCCCCTGCTTTCTGTTTTTCTGTTGGTATTTTTCTCTGTTTCTTATGCATACTAACCGCAGTTGTTTTCTTTTTCTTTCATTTCTGTATTTTTTTTTTTACTATACTTGACATGTCTATGTGTTACCGGTTATTTAAATTTTATTTGAGTTTTTGATTCTATGCAGATAGATATTAATGTGCACCAATTCTTTACCGGGTACACAAATTGTCCCAAGGATAAACAGGATTGGCCTCGAGTATTGAAATTGAAAGATTGGCCTCCTTCTAATTTATTTGAGGAAAGTTTACCTCGTCATTGTGCCGAGTTCATATCTTCCTTGCCATTTAAAGAATATACAAATCCTTTCAAAGGTGCTCTCAACCTTGCTGTGAAGCTGCCTGATAATGTTCTAAAGCCAGACATGGGACCAAAAACATATATAGCTTATGGATTTTCTCAGGAGCTTGGACGGGGTGATTCAGTGACCAAGCTCCATTGTGATATGTCTGATGCAGTATGCTTCAATTCTCTGTGTCTTTCCTGGACTGTTTTTTGTAGATTTTGTTATGAATTTTTTTATGTTTATATTTTTATTTATTATTTGAGTATCATATTGAAAGATGCATAATTTATAGGATGTAAATGATGCCTTTGTATGCCATTGCATTAAGAGCTGATAGTTGTGTAGTTTTAAGTACATCAAATCTTAAATTTTTGGTCATTGATCTGTCTTTGAAACACTCATTTGATTATATATTTGATATATTTGATTTGAAGTGTCATTGACCTTGGTATTTTGGTAACTCATAATTTGAAAGTATTATTTTTCTGATTAGTTGCATCATTTGACAAAGTATTTTTTAGACTTATATGCACTGTCTTTCCCGATTAATTATTTGTTTGTACTTTATATGTTTTGCTTTGTGTCTTGAGTTCTTACTGTATTATGGGGAAAATTAAATTGTTTTGTTTCTTTTACAATTTGGCTTTTAGGTAAATGTGTTGACTCATATTGCTAAAGCAAAATTGGATCCTGCGAGTATTGATGCAATTAAGAAATTGACCCAAAGGCACCTTAAGCAAGACAAGAGGGAGCTACATGGTGATAATCAGGATGGAGAGACCAATTTGTCTTCTTCCATAAATGCTTCAGAGAGCACTGATGATGATGGCCAATCTTCTGGCTCAGAGGTCAAGGAGGTTGATAAAGCGACGATGAAACAAGAAAATGGTTTGTTGGTTGGAGATTCTTTAGATGGTGCTCTCTGGGATATTTTTCGGAGAGAGGACGTACCCGCATTGCAGGAATATCTGAAGAAGCATTTCAAAGAGTTCAGGCACGTCCATTGCTCTCCTTTAGAGGAGGTAAAGTTAGTTGATATGATTTTCTATTATCTGTTTTGATGCCGCTTGTTAACTTGAGTCATTAATGAAACTTAGTTGCTACTAATTAGTTTTGCTGCTGCGAACAAACATGCCATATAGCTACCCCTTTATTTTTCACATTTTGCTGCTGCGAACATGATAAAATTGACACTATCTCATTGCTTTTCCCTATTTGACCTAATTCTTAGCTTATGCTTTATTTTATTTTTCAGTGTTGTTACTGTTGTTTTGCTTATATACCTTGTAATATGTTCTATGGGGCCTTCATAGTTTAGTCTCATAGCCCATTTAACTCCCTATAAATTCCTATCAATAATTTGAGTTACATCAAGTAGTTTAGTTTTCACCTTTTCAAACAAAAGAACCAAAATGCCCCAAATAAAGGTATCGTGCAGCGTGGATTTGGCTTCGTCTGGGCATCACATTGCCTATTGCCTGCTGGCTGCTGCTACCTCGCCTCCTGTGGCGTTGCTTCTTTTTACCTAGCCCGCTCTTTTTGTAGAGACCTTGTGCCTCTCTTCTTTTTTCGTCTACCTAAATCTCTCTTTTTATAGTGTTATGCTTGTTATACTTCTGTACATTGTAATAAGTTGGATTGGTCCTTTGGTTGTTCAGTCCTTCAGCACATTGGTAAGTGCTGCCCAGTGTAAAAGGTTCCAGCACACTTTGTTAGGGGCAACTGTCCATAACAGATTGCAATTCCTATAAATCATTATCGTACTTTCAGTTTTGCGAATAAGCTACATCAACAGAAAGTTATCTCCAGTTATCTGTTTACATTTTCCTTCATTATCTCTTGTTTTCTCAGCTAGTGATTGCTAACATCTGTTAGGTTCAGATTTTTTTAATTATGAAATTTACATGTCCCAAAGGATTGGGCTTGGCGAAGGATCAAGCTGGTAATAAATAATATTTGTGAGTAGTTAATTTTGAATACATACTATCACGAGTAAGGAATTATGTTGATGTGAATAGCGGTATTGAAACATAAGATATGCAGGGAAATGCATTCTGGATGTAATTCCAGTCCAGTAGGTCACTGATTCAGTAGACGTTCTGTATATCTTTCGTTTTACGGTTTCAGTTTCGAAGTTCACTAGGCAGTAGACGGGGGCTTCTTTATTTAGATAACTCGGCAGTCTGGTGACCTTTCACTCCTTATGTATGTATCTTCTTGAATGACATCTCCTTTGGCAATGTAGGTTATTCACCCCATCCATGATCAGACCTTTTATCTAACCGTCCGGCATAAGAAAAAGCTAAAGGAGGAGTATGGTTAGTAGTGCTTATATTTTACTTATGTTGTTTGGTCTGTGGAGTGTAGATCAATAGGCTTGTACCATCTTGTTAACAATTCAACACTGACTTGTATGTAGGAATTGAGCCTTGGACTTTTGTTCAGAAGCTAGGGGATGCTGTTTTCATTCCAGCTGGTTGCCCCCACCAAGTCAGAAATTTGAAGGTAAAATTTACGTACATAGTTGTTTTGTTTTATGTTTAATGAATGCGTAAATTTTCCTTGTTTGCTAAGAAGTGTAGATGTTGATGTTATGAGTTTTACATAATAGAAGAGAAAAAAATAAATATAAATATGGTTAAAGATGATTGATAATGACTTGATGACTAACTATTATGCTACCAAGTTCCTGAAAGTTTGAGAATGTCCTAGTAGTTATATTGTATTCAAAATCCTTATCACATTAATGCTAAATAATTGTCATTTGACAATAATGTGATATTAACTTGATACAAAAGACTAAATGCTAATTCCTAGGTAAAACATTATTACAACAACGTCATAGGTAAAAAATACCTTCCATATTCTGTTATACTTCTTATTATAGAACATTATAATAGGAGATTGCCCAGTTAGTTATTATGCAATTATGTTTTTATTATTATCTGCGTGTGTCGTTTATGTATAGTAGTTATGAGTGTTTCTGTTTAGAATAAAGTAAGAAGGACATAATAGAATTGGTTATATGGAAATTAATTAAGTGGTTATTGGGACTCAAAATCACGGGGAACCAGCATAACCCATCACTGTCAAGTGTGTTCCTGTTCTGTCTTTTCTTGTAACAGTCTGTGTAAAAAATGTATATGTATATGTATTTGTGTGAATCTCTTCTCCAGAGGTTAGATCAATAATATTCCACTGTATTTCATACATAATTATACTTGCTACCAGTTTCTATCATATTCCCATTTAAACATTCCTAAGAGGGGGAGGGGTTTGGGAAAATGGTTCCTCTGAATGTGGGCATGGAATTTAGATTGAGCCTAACTCAATTTGCAAAAAACTAGCTTATAAGTGAGAGATACTTACCACATAAATACATCAAGCCATATCATCCAGCCGTCTAGATCGTTACTCGAGTTGCATGACAATTGTCGCCCAATGAATATAGCATAGCTTCTGATATCTTAGTTTTTAGGTTGGTCTTAACTCAAACCAATGAGTCATTTAAGTTAACGGTTTGAGTGCATCTAAGTTCTTTTTGAAGACTGCATTTTTAAACCTTCAATTTCTTACAGGTTACCCAAGCCATTATCGCTAGCTCACTTTAATGTTCTTGTTAATGCTTGAAAAATTTGTATGAACGTTAATGATATGCATCGTCATTTTTCAGTCATGCACCAAGGTTGCACTTGATTTTGTTTCTCCTGAAAACGTTGGTGAGTGCTTCCGTTTGACAGAGGAATTCCGCAAACTTCCAGTAAATCACAGGTCCATAGAGGACAAATTGGAGGTATGCCAGACTATTATGGATTTAATTCTTCTGTTTATACGCTTACCAAAGCAATTAAGCCTGATTAGAAGCTTTCAGTTAGACCTATTGTTGGCACTGTCATGTTGCAATTGGCCAAATCTGAACACTTCCATTGGTTGAACTGGTTCAAGCATATTATTCACATTTGTTTGAATTGATCATTTTTTACCATCTAGATTCTAGACTGCACAGTGCTATTTAATTCACTAGTAATTATAATGTAACATTAATTTCTGAAAAAATGATGGGAATAGGAAAAAATGGTTATGCATAGTTAGTAGACAATTTAACTTTTTATCGAATGTTTCTTGGAAAAGTCATCAATAGCTCTAGTGAAGAGTATTTGTTTTATATTTTATATTTTTCTATCTATTAGTATTGTAGTTTTTATGCAATGACAAGGTTCTAATATTAACTATTTATACTTGTTGAATGTTTGGCAGTTTTGAGTGTAAAGCTATTTCAACAAACTATGTTACCGTGTCACACCGTTATGTCATATCAATCGATTCTTTATAGTATTTTCTGTAAAGTTTGTTCCTAATTGCTGCTTCTTTTGGTGACAGGTGAAGAAAATGATTATATACACTATGGTTGATGTGGTCAAAACATTAGAGAAAGCATTGGTATGA
Protein sequence

>MS.gene89183.t1

MALAGEDDTLTTASSLNGSCDAVAATERPPILHRYSRKKRRTNATKEDLTKNVEPPTTVKRNRKTEEGASSTTVAAVVVRKSERIRKTNTSYHAGLELQEKPKKGKQSHQNHTKAEKINNTIRRNKWIEEESLMCHQCQRNDKGRVVRCTTCQRKRFCVCCINKWYPHLEEDQIAEACPVCCGNCNCKGCLRSRKLIDSIKQKEDDDTNNDHQVEVSKYMLKALLPHLIRLDQQQMSEKEIEAKIQGRSLSELKIKVSVLHNDERAYCDNCKTSIFDYHRRCTKCSFDLCVLCCRELRCGQLLGGADPFDFEFIFRGQDYLHGGKAEEETRYKAPHAGSELEIRTWSKSGWHADSNGNIPCPKSKNKCDHGYLELRSIFQPNYISDLVCKAKELADSMELQDAEETLDNSCSCLKPVRNRDDIHNNSREAALCVDSRGNFLYHPRAVDLYHEDLGHFQWHWSRGEPVIVSNVLECTSGLSWEPLVMWRAFRQKSKTKHKPLLDVKALECLDWCEIDINVHQFFTGYTNCPKDKQDWPRVLKLKDWPPSNLFEESLPRHCAEFISSLPFKEYTNPFKGALNLAVKLPDNVLKPDMGPKTYIAYGFSQELGRGDSVTKLHCDMSDAVNVLTHIAKAKLDPASIDAIKKLTQRHLKQDKRELHGDNQDGETNLSSSINASESTDDDGQSSGSEVKEVDKATMKQENGLLVGDSLDGALWDIFRREDVPALQEYLKKHFKEFRHVHCSPLEEVIHPIHDQTFYLTVRHKKKLKEEYGIEPWTFVQKLGDAVFIPAGCPHQVRNLKSCTKVALDFVSPENVGECFRLTEEFRKLPVNHRSIEDKLEVKKMIIYTMVDVVKTLEKALV