AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene89213

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene89213.t1 MTR_5g079220 93.717 191 12 0 1 191 86 276 1.24e-119 340
MS.gene89213.t1 MTR_7g017260 51.020 196 74 6 1 187 92 274 1.77e-54 174
MS.gene89213.t1 MTR_5g079290 54.922 193 69 6 1 185 82 264 4.72e-54 173
MS.gene89213.t1 MTR_5g078800 55.897 195 68 8 1 184 72 259 6.61e-53 170
MS.gene89213.t1 MTR_0001s0360 53.125 192 74 8 1 187 72 252 5.11e-52 167
MS.gene89213.t1 MTR_5g078950 49.510 204 74 7 1 185 72 265 1.95e-45 151
MS.gene89213.t1 MTR_5g079120 47.449 196 78 7 1 187 126 305 2.29e-44 149
MS.gene89213.t1 MTR_5g078930 57.252 131 46 3 1 127 74 198 4.31e-44 146
MS.gene89213.t1 MTR_5g078860 46.320 231 74 9 1 183 72 300 2.67e-43 146
MS.gene89213.t1 MTR_5g078910 45.148 237 70 7 1 187 72 298 7.08e-42 142
MS.gene89213.t1 MTR_8g060940 49.231 195 67 6 1 187 72 242 3.43e-37 129
MS.gene89213.t1 MTR_5g079140 53.782 119 45 3 13 127 7 119 1.47e-35 122
MS.gene89213.t1 MTR_0023s0260 46.857 175 77 6 20 187 10 175 3.07e-33 117
MS.gene89213.t1 MTR_0193s0090 47.656 128 53 3 1 127 72 186 1.48e-29 108
MS.gene89213.t1 MTR_0247s0040 52.542 118 46 1 1 118 72 179 1.79e-26 100
MS.gene89213.t1 MTR_0197s0010 52.703 74 31 1 1 74 66 135 9.22e-20 83.6
MS.gene89213.t1 MTR_4g125520 50.685 73 36 0 1 73 77 149 1.33e-18 81.6
MS.gene89213.t1 MTR_4g100720 70.000 50 14 1 1 50 78 126 7.16e-16 72.0
MS.gene89213.t1 MTR_3g083540 67.442 43 14 0 1 43 106 148 2.24e-15 72.8
MS.gene89213.t1 MTR_8g027345 55.932 59 20 1 1 53 77 135 4.61e-15 72.0
MS.gene89213.t1 MTR_4g485530 65.116 43 15 0 1 43 77 119 7.22e-15 69.7
MS.gene89213.t1 MTR_3g097450 46.154 65 35 0 1 65 77 141 7.95e-15 71.2
MS.gene89213.t1 MTR_1g076150 58.182 55 21 1 2 54 78 132 1.15e-14 70.5
MS.gene89213.t1 MTR_4g073420 40.000 100 49 2 1 100 77 165 1.74e-14 70.1
MS.gene89213.t1 MTR_7g115650 71.795 39 11 0 2 40 78 116 1.99e-14 68.9
MS.gene89213.t1 MTR_1g043050 52.542 59 27 1 1 58 77 135 2.06e-14 69.7
MS.gene89213.t1 MTR_5g079670 63.415 41 15 0 1 41 76 116 3.06e-14 68.6
MS.gene89213.t1 MTR_7g117730 60.000 45 18 0 1 45 77 121 3.47e-14 68.9
MS.gene89213.t1 MTR_8g020490 72.500 40 11 0 1 40 73 112 4.45e-14 68.9
MS.gene89213.t1 MTR_0251s0050 54.545 66 24 2 1 62 78 141 4.87e-14 68.9
MS.gene89213.t1 MTR_7g096930 28.426 197 98 6 2 178 78 251 1.66e-13 67.4
MS.gene89213.t1 MTR_0008s0390 60.784 51 16 1 1 47 78 128 4.14e-13 66.2
MS.gene89213.t1 MTR_6g055910 56.818 44 19 0 2 45 10 53 4.48e-13 65.5
MS.gene89213.t1 MTR_2g089620 65.000 40 14 0 2 41 78 117 5.16e-13 66.6
MS.gene89213.t1 MTR_4g121460 38.824 85 50 1 1 83 77 161 5.98e-13 66.2
MS.gene89213.t1 MTR_0008s0470 49.296 71 31 2 1 66 78 148 6.25e-13 65.9
MS.gene89213.t1 MTR_1g100653 50.000 58 29 0 2 59 78 135 6.63e-13 65.9
MS.gene89213.t1 MTR_2g089450 44.118 68 37 1 2 68 78 145 6.88e-13 65.5
MS.gene89213.t1 MTR_2g011660 62.500 40 15 0 2 41 79 118 6.88e-13 66.2
MS.gene89213.t1 MTR_1g043080 62.500 40 15 0 2 41 78 117 7.24e-13 65.5
MS.gene89213.t1 MTR_4g091490 62.500 40 15 0 2 41 79 118 1.47e-12 65.5
MS.gene89213.t1 MTR_2g089420 40.000 70 42 0 1 70 76 145 1.49e-12 65.5
MS.gene89213.t1 MTR_3g039990 52.632 57 27 0 1 57 78 134 1.59e-12 65.1
MS.gene89213.t1 MTR_6g055910 56.818 44 19 0 2 45 51 94 1.70e-12 64.3
MS.gene89213.t1 MTR_2g034790 44.444 63 35 0 1 63 77 139 2.36e-12 64.7
MS.gene89213.t1 MTR_6g055910 56.818 44 19 0 2 45 80 123 3.13e-12 63.9
MS.gene89213.t1 MTR_4g063100 53.333 45 21 0 1 45 8 52 4.89e-12 62.0
MS.gene89213.t1 MTR_7g076740 39.726 73 43 1 1 73 78 149 5.46e-12 63.5
MS.gene89213.t1 MTR_0008s0280 49.206 63 30 1 1 63 77 137 7.46e-12 62.4
MS.gene89213.t1 MTR_3g461490 66.667 39 13 0 2 40 78 116 7.62e-12 63.2
MS.gene89213.t1 MTR_4g082290 65.000 40 14 0 2 41 78 117 8.57e-12 63.2
MS.gene89213.t1 MTR_4g082230 65.000 40 14 0 2 41 78 117 8.99e-12 63.2
MS.gene89213.t1 MTR_6g009430 63.415 41 15 0 1 41 77 117 1.29e-11 62.4
MS.gene89213.t1 MTR_3g074520 60.000 40 16 0 2 41 78 117 1.48e-11 62.4
MS.gene89213.t1 MTR_5g014990 46.154 52 28 0 1 52 78 129 1.49e-11 62.4
MS.gene89213.t1 MTR_4g063100 53.333 45 21 0 1 45 43 87 1.51e-11 61.2
MS.gene89213.t1 MTR_2g088730 65.625 32 11 0 1 32 41 72 1.55e-11 58.2
MS.gene89213.t1 MTR_4g063100 53.333 45 21 0 1 45 72 116 1.75e-11 61.6
MS.gene89213.t1 MTR_2g096380 56.818 44 19 0 1 44 8 51 1.75e-11 60.5
MS.gene89213.t1 MTR_7g011170 62.500 40 15 0 2 41 78 117 2.15e-11 61.6
MS.gene89213.t1 MTR_7g010210 62.500 40 15 0 1 40 77 116 2.46e-11 61.6
MS.gene89213.t1 MTR_8g031360 53.659 41 19 0 1 41 79 119 2.61e-11 61.6
MS.gene89213.t1 MTR_8g095390 51.163 43 21 0 1 43 77 119 2.98e-11 61.2
MS.gene89213.t1 MTR_4g102380 56.098 41 18 0 1 41 77 117 3.24e-11 60.8
MS.gene89213.t1 MTR_7g010210 62.500 40 15 0 1 40 77 116 3.41e-11 60.8
MS.gene89213.t1 MTR_1g100667 56.818 44 19 0 2 45 79 122 3.54e-11 60.8
MS.gene89213.t1 MTR_0140s0030 62.500 40 15 0 1 40 78 117 5.34e-11 60.8
MS.gene89213.t1 MTR_0489s0020 62.500 40 15 0 1 40 78 117 5.34e-11 60.8
MS.gene89213.t1 MTR_4g065017 61.538 39 15 0 2 40 83 121 5.35e-11 60.8
MS.gene89213.t1 MTR_5g029840 51.852 54 25 1 1 54 77 129 5.82e-11 60.5
MS.gene89213.t1 MTR_0063s0090 55.814 43 19 0 1 43 77 119 6.28e-11 59.7
MS.gene89213.t1 MTR_2g097910 57.500 40 17 0 1 40 85 124 6.39e-11 60.5
MS.gene89213.t1 MTR_2g096380 56.818 44 19 0 1 44 71 114 7.40e-11 59.7
MS.gene89213.t1 MTR_4g478180 57.500 40 17 0 2 41 79 118 9.16e-11 60.1
MS.gene89213.t1 MTR_1g017140 47.059 51 27 0 1 51 78 128 9.69e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene89213.t1 AT1G66370 43.333 150 58 5 1 136 73 209 2.08e-24 96.3
MS.gene89213.t1 AT3G13540 41.228 114 54 4 1 114 88 188 2.65e-17 77.4
MS.gene89213.t1 AT5G14750 65.306 49 17 0 1 49 20 68 1.51e-16 73.2
MS.gene89213.t1 AT5G14750 65.306 49 17 0 1 49 20 68 1.51e-16 73.2
MS.gene89213.t1 AT5G14750 65.306 49 17 0 1 49 47 95 5.16e-16 72.4
MS.gene89213.t1 AT5G52600 64.000 50 18 0 1 50 77 126 8.44e-16 72.4
MS.gene89213.t1 AT5G14750 65.306 49 17 0 1 49 81 129 1.34e-15 72.0
MS.gene89213.t1 AT3G27920 73.810 42 11 0 1 42 79 120 2.44e-15 72.0
MS.gene89213.t1 AT2G16720 65.116 43 15 0 1 43 77 119 4.83e-15 71.6
MS.gene89213.t1 AT1G66390 38.621 145 53 5 10 136 82 208 8.94e-15 70.5
MS.gene89213.t1 AT1G22640 60.465 43 17 0 1 43 20 62 1.06e-14 69.7
MS.gene89213.t1 AT1G56650 36.774 155 69 6 10 152 82 219 1.09e-14 70.5
MS.gene89213.t1 AT3G23250 54.237 59 21 1 2 54 21 79 1.23e-14 70.1
MS.gene89213.t1 AT5G40330 75.000 40 10 0 1 40 77 116 1.27e-14 69.7
MS.gene89213.t1 AT4G38620 60.465 43 17 0 1 43 77 119 1.30e-14 70.5
MS.gene89213.t1 AT4G09460 35.043 117 68 3 1 116 77 186 2.76e-14 68.9
MS.gene89213.t1 AT1G22640 60.465 43 17 0 1 43 77 119 4.10e-14 68.9
MS.gene89213.t1 AT1G35515 31.897 116 68 2 1 115 77 182 7.72e-14 67.4
MS.gene89213.t1 AT3G23250 54.237 59 21 1 2 54 78 136 7.84e-14 68.6
MS.gene89213.t1 AT4G34990 60.465 43 17 0 1 43 77 119 1.58e-13 67.4
MS.gene89213.t1 AT3G30210 48.611 72 34 2 1 71 92 161 1.76e-13 67.4
MS.gene89213.t1 AT5G35550 55.357 56 23 1 1 56 82 135 2.04e-13 67.0
MS.gene89213.t1 AT5G35550 55.357 56 23 1 1 56 79 132 4.55e-13 66.2
MS.gene89213.t1 AT1G06180 60.000 45 18 0 2 46 78 122 4.56e-13 65.9
MS.gene89213.t1 AT4G05100 62.500 40 15 0 2 41 79 118 4.62e-13 66.6
MS.gene89213.t1 AT4G28110 62.500 40 15 0 2 41 78 117 7.31e-13 65.9
MS.gene89213.t1 AT4G21440 62.500 40 15 0 2 41 78 117 1.17e-12 65.5
MS.gene89213.t1 AT4G17785 65.000 40 14 0 2 41 79 118 1.18e-12 65.5
MS.gene89213.t1 AT4G17785 65.000 40 14 0 2 41 79 118 1.22e-12 65.5
MS.gene89213.t1 AT1G74650 61.364 44 17 0 1 44 1 44 2.11e-12 64.3
MS.gene89213.t1 AT5G59780 59.091 44 18 0 1 44 8 51 2.22e-12 62.8
MS.gene89213.t1 AT4G12350 60.976 41 16 0 1 41 77 117 2.52e-12 64.3
MS.gene89213.t1 AT3G61250 49.020 51 26 0 1 51 77 127 2.83e-12 64.3
MS.gene89213.t1 AT2G31180 66.667 39 13 0 2 40 78 116 3.30e-12 63.5
MS.gene89213.t1 AT5G65790 45.614 57 31 0 1 57 78 134 3.48e-12 64.3
MS.gene89213.t1 AT5G49330 59.524 42 17 0 1 42 77 118 3.53e-12 64.3
MS.gene89213.t1 AT3G46130 59.091 44 18 0 1 44 8 51 3.75e-12 62.4
MS.gene89213.t1 AT1G71030 63.415 41 15 0 1 41 31 71 4.50e-12 62.0
MS.gene89213.t1 AT1G71030 63.415 41 15 0 1 41 45 85 5.97e-12 62.0
MS.gene89213.t1 AT5G56110 67.500 40 13 0 1 40 77 116 6.07e-12 63.5
MS.gene89213.t1 AT3G02940 62.500 40 15 0 2 41 78 117 6.08e-12 63.5
MS.gene89213.t1 AT3G02940 62.500 40 15 0 2 41 78 117 6.10e-12 63.5
MS.gene89213.t1 AT3G02940 62.500 40 15 0 2 41 78 117 6.10e-12 63.5
MS.gene89213.t1 AT5G16770 65.000 40 14 0 2 41 78 117 6.64e-12 63.5
MS.gene89213.t1 AT5G16770 65.000 40 14 0 2 41 78 117 6.64e-12 63.5
MS.gene89213.t1 AT5G16770 65.000 40 14 0 2 41 78 117 6.73e-12 63.5
MS.gene89213.t1 AT5G16770 65.000 40 14 0 2 41 78 117 6.73e-12 63.5
MS.gene89213.t1 AT1G74650 61.364 44 17 0 1 44 77 120 7.25e-12 63.2
MS.gene89213.t1 AT5G65230 60.000 40 16 0 2 41 78 117 7.39e-12 63.2
MS.gene89213.t1 AT1G66380 89.655 29 3 0 10 38 49 77 8.42e-12 59.7
MS.gene89213.t1 AT5G15310 51.667 60 25 1 2 57 78 137 9.10e-12 63.2
MS.gene89213.t1 AT5G54230 60.000 40 16 0 2 41 78 117 9.64e-12 62.8
MS.gene89213.t1 AT5G59780 59.091 44 18 0 1 44 52 95 1.01e-11 61.6
MS.gene89213.t1 AT1G16490 57.143 42 18 0 2 43 80 121 1.03e-11 62.4
MS.gene89213.t1 AT5G15310 51.667 60 25 1 2 57 78 137 1.07e-11 62.8
MS.gene89213.t1 AT3G53200 65.789 38 13 0 1 38 41 78 1.15e-11 61.6
MS.gene89213.t1 AT3G46130 57.778 45 19 0 1 45 42 86 1.28e-11 61.6
MS.gene89213.t1 AT5G59780 59.091 44 18 0 1 44 73 116 1.38e-11 61.6
MS.gene89213.t1 AT3G46130 57.778 45 19 0 1 45 52 96 1.43e-11 61.6
MS.gene89213.t1 AT3G28910 62.500 40 15 0 1 40 1 40 1.72e-11 61.6
MS.gene89213.t1 AT3G53200 58.140 43 18 0 1 43 74 116 1.75e-11 61.6
MS.gene89213.t1 AT3G46130 57.778 45 19 0 1 45 74 118 1.84e-11 61.6
MS.gene89213.t1 AT1G66380 89.655 29 3 0 10 38 82 110 2.09e-11 59.3
MS.gene89213.t1 AT3G01140 62.500 40 15 0 2 41 78 117 2.10e-11 62.0
MS.gene89213.t1 AT3G01140 62.500 40 15 0 2 41 121 160 2.33e-11 62.0
MS.gene89213.t1 AT5G15310 51.667 60 25 1 2 57 78 137 2.39e-11 61.2
MS.gene89213.t1 AT5G10280 60.000 40 16 0 2 41 78 117 2.61e-11 61.6
MS.gene89213.t1 AT3G47600 65.000 40 14 0 1 40 77 116 2.89e-11 61.6
MS.gene89213.t1 AT3G47600 65.000 40 14 0 1 40 71 110 2.92e-11 61.6
MS.gene89213.t1 AT5G12870 57.500 40 17 0 1 40 83 122 3.00e-11 61.2
MS.gene89213.t1 AT4G25560 64.865 37 13 0 2 38 10 46 3.49e-11 60.5
MS.gene89213.t1 AT2G47460 57.500 40 17 0 1 40 77 116 3.87e-11 61.2
MS.gene89213.t1 AT5G62320 56.098 41 18 0 1 41 85 125 4.34e-11 60.5
MS.gene89213.t1 AT3G49690 51.064 47 23 0 1 47 78 124 4.73e-11 60.8
MS.gene89213.t1 AT3G28910 62.500 40 15 0 1 40 77 116 5.99e-11 60.5
MS.gene89213.t1 AT3G48920 52.174 46 22 0 1 46 83 128 6.75e-11 60.1
MS.gene89213.t1 AT5G57620 52.174 46 22 0 1 46 78 123 6.75e-11 60.5
MS.gene89213.t1 AT4G37780 30.435 138 83 5 1 126 78 214 7.30e-11 60.5
MS.gene89213.t1 AT1G18570 57.143 42 18 0 2 43 79 120 7.98e-11 60.5
MS.gene89213.t1 AT1G57560 55.814 43 19 0 1 43 77 119 8.19e-11 60.1

Find 35 sgRNAs with CRISPR-Local

Find 68 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTCATTCACCATGGTTAAA+TGG 0.170730 5.2:+67446799 MS.gene89213:CDS
AAAGTTATCAACATTTAATT+TGG 0.172381 5.2:-67447049 None:intergenic
GTACTGTCGGTGATGGCTTC+TGG 0.187087 5.2:+67447088 MS.gene89213:CDS
TTTAGATGATATCTTAAATT+AGG 0.200033 5.2:+67447140 MS.gene89213:CDS
CACACATTTGCGCAAGAATA+TGG 0.270216 5.2:+67446684 MS.gene89213:CDS
CAATCTCCATCAACATTAAT+TGG 0.275334 5.2:-67446910 None:intergenic
CTAATGATGTGAAGAATTAT+TGG 0.279813 5.2:+67446659 MS.gene89213:CDS
ATTCTACCAATTAATGTTGA+TGG 0.309304 5.2:+67446904 MS.gene89213:CDS
TGAGTTTGCATGCTTTCTTT+AGG 0.316758 5.2:-67446742 None:intergenic
TACTGTCGGTGATGGCTTCT+GGG 0.330685 5.2:+67447089 MS.gene89213:CDS
TTGATTGCTGCAAGACTTCC+GGG 0.345324 5.2:+67446628 MS.gene89213:CDS
CTCATTCACCATGGTTAAAT+GGG 0.379865 5.2:+67446800 MS.gene89213:CDS
ATTGATTGCTGCAAGACTTC+CGG 0.392491 5.2:+67446627 MS.gene89213:CDS
TTTGTTGAAACCGTCACTAT+TGG 0.404174 5.2:-67446844 None:intergenic
TTTAATTTGGAATATTCCTC+TGG 0.409911 5.2:-67447036 None:intergenic
GAAGCCATCACCGACAGTAC+TGG 0.437278 5.2:-67447085 None:intergenic
TACACAAGCTCCTAGGAAAC+AGG 0.453563 5.2:+67445844 MS.gene89213:CDS
AACGTGAGCAATGACAAAAT+TGG 0.485163 5.2:+67446994 MS.gene89213:CDS
TTAAGGTTACACAAGCTCCT+AGG 0.491730 5.2:+67445837 MS.gene89213:CDS
GTTGATAACTTTCTTACTGA+AGG 0.494299 5.2:+67447060 MS.gene89213:CDS
AAGAGAGTCAAATTCACAAA+TGG 0.496518 5.2:-67447118 None:intergenic
TGCATACATACCTGTTTCCT+AGG 0.499222 5.2:-67445854 None:intergenic
CAACCGTGTCTTCCATGTGG+TGG 0.559880 5.2:+67446959 MS.gene89213:CDS
AAGCCATCACCGACAGTACT+GGG 0.562213 5.2:-67447084 None:intergenic
AACCGTGTCTTCCATGTGGT+GGG 0.571636 5.2:+67446960 MS.gene89213:CDS
GGACCCAGTACTGTCGGTGA+TGG 0.574154 5.2:+67447081 MS.gene89213:CDS
ACATCATTAGCTGTTCTACC+CGG 0.596176 5.2:-67446646 None:intergenic
CTCCCACCACATGGAAGACA+CGG 0.597007 5.2:-67446962 None:intergenic
TTATATTTCCCATTTAACCA+TGG 0.601242 5.2:-67446808 None:intergenic
ATTTGTCACACCAATAGTGA+CGG 0.606503 5.2:+67446834 MS.gene89213:CDS
CAACAAACTCTCCCACCACA+TGG 0.617722 5.2:-67446971 None:intergenic
ATGCTCTTTACTATTACCAG+AGG 0.637280 5.2:+67447020 MS.gene89213:CDS
ACTGAAGGACCCAGTACTGT+CGG 0.653910 5.2:+67447075 MS.gene89213:CDS
GTGACGGTTTCAACAAACGA+TGG 0.707992 5.2:+67446850 MS.gene89213:CDS
ATTCAACCGTGTCTTCCATG+TGG 0.728421 5.2:+67446956 MS.gene89213:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATCAAATAAACTTATATTTA+AGG - chr5.2:67445995-67446014 None:intergenic 10.0%
!! AAAGTTATCAACATTTAATT+TGG - chr5.2:67447052-67447071 None:intergenic 15.0%
!! TAAATGAGTATAAAAAGTTT+GGG + chr5.2:67446448-67446467 MS.gene89213:intron 15.0%
!! TATTAATCAAAGGTTTATTT+GGG - chr5.2:67446235-67446254 None:intergenic 15.0%
!! TATTCTTAAAATAGTTTAAG+TGG - chr5.2:67446551-67446570 None:intergenic 15.0%
!! TTAAATGAGTATAAAAAGTT+TGG + chr5.2:67446447-67446466 MS.gene89213:intron 15.0%
!!! ATTTAAAATTAGTCCTTTTT+AGG - chr5.2:67446183-67446202 None:intergenic 15.0%
!!! TTTAAAATTAGTCCTTTTTA+GGG - chr5.2:67446182-67446201 None:intergenic 15.0%
!! AAAGTGGCATTATTATATAT+TGG - chr5.2:67446105-67446124 None:intergenic 20.0%
!! CTAAGAGATATTCATAAAAA+AGG + chr5.2:67446277-67446296 MS.gene89213:intron 20.0%
!! GTATTAATCAAAGGTTTATT+TGG - chr5.2:67446236-67446255 None:intergenic 20.0%
!! GTGAAATATTATTGATAAAG+TGG - chr5.2:67446121-67446140 None:intergenic 20.0%
!! TAACTAGTAGTATTAATCAA+AGG - chr5.2:67446245-67446264 None:intergenic 20.0%
!! TATCTAATTAAATGACAACT+TGG - chr5.2:67445891-67445910 None:intergenic 20.0%
! ATTCTACCAATTAATGTTGA+TGG + chr5.2:67446904-67446923 MS.gene89213:CDS 25.0%
! CTAATGATGTGAAGAATTAT+TGG + chr5.2:67446659-67446678 MS.gene89213:CDS 25.0%
! TTATATTTCCCATTTAACCA+TGG - chr5.2:67446811-67446830 None:intergenic 25.0%
!! AATAAAGAAGAAAAGTGTGA+TGG + chr5.2:67446365-67446384 MS.gene89213:intron 25.0%
!! AATTCAAATTTTCTCGAAGA+TGG + chr5.2:67446067-67446086 MS.gene89213:intron 25.0%
!! ATAAAGAAGAAAAGTGTGAT+GGG + chr5.2:67446366-67446385 MS.gene89213:intron 25.0%
!! ATTCAAATTTTCTCGAAGAT+GGG + chr5.2:67446068-67446087 MS.gene89213:intron 25.0%
!!! TTTAATTTGGAATATTCCTC+TGG - chr5.2:67447039-67447058 None:intergenic 25.0%
AAGAGAGTCAAATTCACAAA+TGG - chr5.2:67447121-67447140 None:intergenic 30.0%
ACAAGTGTCTTAAAATATGG+GGG + chr5.2:67446300-67446319 MS.gene89213:intron 30.0%
AGGACAAGTGTCTTAAAATA+TGG + chr5.2:67446297-67446316 MS.gene89213:intron 30.0%
CAATCTCCATCAACATTAAT+TGG - chr5.2:67446913-67446932 None:intergenic 30.0%
GACAAGTGTCTTAAAATATG+GGG + chr5.2:67446299-67446318 MS.gene89213:intron 30.0%
GGACAAGTGTCTTAAAATAT+GGG + chr5.2:67446298-67446317 MS.gene89213:intron 30.0%
!! ACGGTTGAATTTTCAAGATA+TGG - chr5.2:67446946-67446965 None:intergenic 30.0%
!! ATTTTCAAGATATGGTTGTG+CGG - chr5.2:67446938-67446957 None:intergenic 30.0%
!! GTTGATAACTTTCTTACTGA+AGG + chr5.2:67447060-67447079 MS.gene89213:CDS 30.0%
!!! AGCTTGTTTTGTAATCTGAA+GGG + chr5.2:67446040-67446059 MS.gene89213:intron 30.0%
!!! TGTGTCTTTTTTCCCTAAAA+AGG + chr5.2:67446167-67446186 MS.gene89213:intron 30.0%
AACGTGAGCAATGACAAAAT+TGG + chr5.2:67446994-67447013 MS.gene89213:CDS 35.0%
ATGCTCTTTACTATTACCAG+AGG + chr5.2:67447020-67447039 MS.gene89213:CDS 35.0%
ATTAAATGACAACTTGGCCT+TGG - chr5.2:67445885-67445904 None:intergenic 35.0%
ATTTGTCACACCAATAGTGA+CGG + chr5.2:67446834-67446853 MS.gene89213:CDS 35.0%
CTCATTCACCATGGTTAAAT+GGG + chr5.2:67446800-67446819 MS.gene89213:CDS 35.0%
GAATGAGATGAAAAAGTTCG+AGG - chr5.2:67446787-67446806 None:intergenic 35.0%
TCTCATTCACCATGGTTAAA+TGG + chr5.2:67446799-67446818 MS.gene89213:CDS 35.0%
TGAGTTTGCATGCTTTCTTT+AGG - chr5.2:67446745-67446764 None:intergenic 35.0%
TGGGGGAAGTCAATATTAAA+TGG + chr5.2:67446317-67446336 MS.gene89213:intron 35.0%
TTTGTTGAAACCGTCACTAT+TGG - chr5.2:67446847-67446866 None:intergenic 35.0%
!! CTTTTTCATCTCATTCACCA+TGG + chr5.2:67446791-67446810 MS.gene89213:CDS 35.0%
!!! GAGCTTGTTTTGTAATCTGA+AGG + chr5.2:67446039-67446058 MS.gene89213:intron 35.0%
ACATCATTAGCTGTTCTACC+CGG - chr5.2:67446649-67446668 None:intergenic 40.0%
ATTGATTGCTGCAAGACTTC+CGG + chr5.2:67446627-67446646 MS.gene89213:CDS 40.0%
CACACATTTGCGCAAGAATA+TGG + chr5.2:67446684-67446703 MS.gene89213:CDS 40.0%
GATGAAAAAGTTCGAGGTTG+AGG - chr5.2:67446781-67446800 None:intergenic 40.0%
TGCATACATACCTGTTTCCT+AGG - chr5.2:67445857-67445876 None:intergenic 40.0%
TTAAGGTTACACAAGCTCCT+AGG + chr5.2:67445837-67445856 MS.gene89213:CDS 40.0%
ATTCAACCGTGTCTTCCATG+TGG + chr5.2:67446956-67446975 MS.gene89213:CDS 45.0%
GTGACGGTTTCAACAAACGA+TGG + chr5.2:67446850-67446869 MS.gene89213:CDS 45.0%
TACACAAGCTCCTAGGAAAC+AGG + chr5.2:67445844-67445863 MS.gene89213:CDS 45.0%
TTGATTGCTGCAAGACTTCC+GGG + chr5.2:67446628-67446647 MS.gene89213:CDS 45.0%
!!! TATTTTTATATTTTTATAGA+TGG + chr5.2:67446602-67446621 MS.gene89213:intron 5.0%
!!! TTAAGAATATAATATTTTTT+TGG - chr5.2:67445962-67445981 None:intergenic 5.0%
AACCGTGTCTTCCATGTGGT+GGG + chr5.2:67446960-67446979 MS.gene89213:CDS 50.0%
AAGCCATCACCGACAGTACT+GGG - chr5.2:67447087-67447106 None:intergenic 50.0%
ACTGAAGGACCCAGTACTGT+CGG + chr5.2:67447075-67447094 MS.gene89213:CDS 50.0%
CAACAAACTCTCCCACCACA+TGG - chr5.2:67446974-67446993 None:intergenic 50.0%
GGTATGTATGCATGCTGCCA+AGG + chr5.2:67445865-67445884 MS.gene89213:intron 50.0%
! TACTGTCGGTGATGGCTTCT+GGG + chr5.2:67447089-67447108 MS.gene89213:CDS 50.0%
CAACCGTGTCTTCCATGTGG+TGG + chr5.2:67446959-67446978 MS.gene89213:CDS 55.0%
CTCCCACCACATGGAAGACA+CGG - chr5.2:67446965-67446984 None:intergenic 55.0%
GAAGCCATCACCGACAGTAC+TGG - chr5.2:67447088-67447107 None:intergenic 55.0%
! GTACTGTCGGTGATGGCTTC+TGG + chr5.2:67447088-67447107 MS.gene89213:CDS 55.0%
GGACCCAGTACTGTCGGTGA+TGG + chr5.2:67447081-67447100 MS.gene89213:CDS 60.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 67445831 67447161 67445831 ID=MS.gene89213
chr5.2 mRNA 67445831 67447161 67445831 ID=MS.gene89213.t1;Parent=MS.gene89213
chr5.2 exon 67445831 67445865 67445831 ID=MS.gene89213.t1.exon1;Parent=MS.gene89213.t1
chr5.2 CDS 67445831 67445865 67445831 ID=cds.MS.gene89213.t1;Parent=MS.gene89213.t1
chr5.2 exon 67446621 67447161 67446621 ID=MS.gene89213.t1.exon2;Parent=MS.gene89213.t1
chr5.2 CDS 67446621 67447161 67446621 ID=cds.MS.gene89213.t1;Parent=MS.gene89213.t1
Gene Sequence

>MS.gene89213

ATGATTTTAAGGTTACACAAGCTCCTAGGAAACAGGTATGTATGCATGCTGCCAAGGCCAAGTTGTCATTTAATTAGATATTTTCTTATAATTCAATTTTAGCAGTTATTATATTCTTAATATCACTACCAAAAAAATATTATATTCTTAATATTATGTTTCCTTAAATATAAGTTTATTTGATCATAAAGTGTAATCTTAGTAGTATGAGCTTGTTTTGTAATCTGAAGGGTCAAAATTCAAATTTTCTCGAAGATGGGTGTGAAAAAGACCAATATATAATAATGCCACTTTATCAATAATATTTCACTTGCTTAATTTTTATAAAAAAAAGTATGTGTCTTTTTTCCCTAAAAAGGACTAATTTTAAATTACTAGATGTACATACAACTAATTTTTTTCCCAAATAAACCTTTGATTAATACTACTAGTTAAGTGTCTTAGATCTAAGAGATATTCATAAAAAAGGACAAGTGTCTTAAAATATGGGGGAAGTCAATATTAAATGGAGAATGTTTAGTGTACATAAAGAAAAATAAAGAAGAAAAGTGTGATGGGATCGATGTAATATGTTGATGATGTGATAAGATGAACGAGATAGAGATACAATAAAATATTAAATGAGTATAAAAAGTTTGGGTGTTCAATATCATTATTGATATTAAATACATAAAGTTATAAAATAGTATTTCAAGATTTTTATAAAAAAGAATGTTACCACTTAAACTATTTTAAGAATAATTTAATTTTATGAAGTACTAGTTAACATAATATTTTTATATTTTTATAGATGGTCATTGATTGCTGCAAGACTTCCGGGTAGAACAGCTAATGATGTGAAGAATTATTGGCACACACATTTGCGCAAGAATATGGTTTCAAAAAAAGAAGAAAAGAAAGAAAATGAGAAACCTAAAGAAAGCATGCAAACTCATGAAGTGATTAAACCTCAACCTCGAACTTTTTCATCTCATTCACCATGGTTAAATGGGAAATATAATAAATTTGTCACACCAATAGTGACGGTTTCAACAAACGATGGTACTGTTGCAAAAGATAGTGAAGTAGATACTATTCTACCAATTAATGTTGATGGAGATTGTGCCGCACAACCATATCTTGAAAATTCAACCGTGTCTTCCATGTGGTGGGAGAGTTTGTTGAACGTGAGCAATGACAAAATTGGATCATGCTCTTTACTATTACCAGAGGAATATTCCAAATTAAATGTTGATAACTTTCTTACTGAAGGACCCAGTACTGTCGGTGATGGCTTCTGGGATTCTACCATTTGTGAATTTGACTCTCTTTTAGATGATATCTTAAATTAG
Protein sequence

>MS.gene89213.t1

MILRLHKLLGNRWSLIAARLPGRTANDVKNYWHTHLRKNMVSKKEEKKENEKPKESMQTHEVIKPQPRTFSSHSPWLNGKYNKFVTPIVTVSTNDGTVAKDSEVDTILPINVDGDCAAQPYLENSTVSSMWWESLLNVSNDKIGSCSLLLPEEYSKLNVDNFLTEGPSTVGDGFWDSTICEFDSLLDDILN