Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89314.t1 | XP_024626703.1 | 85.7 | 168 | 23 | 1 | 1 | 168 | 1 | 167 | 4.80E-76 | 293.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89314.t1 | Q39266 | 37.5 | 104 | 59 | 3 | 34 | 136 | 33 | 131 | 5.6e-09 | 62.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89314.t1 | A0A396GYM7 | 85.7 | 168 | 23 | 1 | 1 | 168 | 1 | 167 | 3.4e-76 | 293.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene89314.t1 | TF | C2H2 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89314.t1 | MTR_2g067740 | 76.331 | 169 | 39 | 1 | 1 | 168 | 1 | 169 | 6.21e-79 | 233 |
| MS.gene89314.t1 | MTR_3g012010 | 39.474 | 114 | 54 | 5 | 58 | 160 | 98 | 207 | 1.11e-13 | 67.4 |
| MS.gene89314.t1 | MTR_3g009410 | 49.206 | 63 | 31 | 1 | 58 | 119 | 98 | 160 | 1.61e-12 | 64.3 |
| MS.gene89314.t1 | MTR_7g022980 | 36.275 | 102 | 59 | 3 | 55 | 153 | 63 | 161 | 5.99e-12 | 62.4 |
| MS.gene89314.t1 | MTR_5g008280 | 37.719 | 114 | 55 | 7 | 57 | 160 | 45 | 152 | 1.58e-11 | 61.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89314.t1 | AT1G24625 | 37.143 | 105 | 60 | 3 | 34 | 137 | 33 | 132 | 2.83e-13 | 65.5 |
| MS.gene89314.t1 | AT5G01860 | 34.579 | 107 | 56 | 2 | 11 | 117 | 24 | 116 | 2.11e-11 | 60.5 |
Find 52 sgRNAs with CRISPR-Local
Find 60 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTGAATAATAATGTGGGATT+TGG | 0.253595 | 7.1:-38047759 | MS.gene89314:CDS |
| CTCATGGATCTTGAGGGATT+TGG | 0.283300 | 7.1:+38048091 | None:intergenic |
| CATTATTATTCAGTGGAGTT+TGG | 0.293367 | 7.1:+38047769 | None:intergenic |
| CCTCCAACAGTGAAGGGATA+TGG | 0.296765 | 7.1:+38047891 | None:intergenic |
| TATGGGTGATAAATTGAATA+TGG | 0.313679 | 7.1:+38047909 | None:intergenic |
| GAAATTCAATTAGGAACGTT+TGG | 0.323330 | 7.1:-38047954 | MS.gene89314:CDS |
| ATTATTATTCAGTGGAGTTT+GGG | 0.334063 | 7.1:+38047770 | None:intergenic |
| CAATTAGGAACGTTTGGACT+TGG | 0.342211 | 7.1:-38047948 | MS.gene89314:CDS |
| ACGTCATTGTCTCTGAGTTT+AGG | 0.351844 | 7.1:-38048140 | MS.gene89314:CDS |
| GCAAGCTATGACAACCCATT+TGG | 0.366612 | 7.1:-38047819 | MS.gene89314:CDS |
| TCATAGCTTGCATGGGCAAC+AGG | 0.381549 | 7.1:+38047831 | None:intergenic |
| CAAGCACTCTTTCACGCCTA+TGG | 0.392813 | 7.1:+38047991 | None:intergenic |
| CAAGCTATGACAACCCATTT+GGG | 0.409161 | 7.1:-38047818 | MS.gene89314:CDS |
| CCATATCCCTTCACTGTTGG+AGG | 0.415111 | 7.1:-38047891 | MS.gene89314:CDS |
| CACCCATATCCCTTCACTGT+TGG | 0.446404 | 7.1:-38047894 | MS.gene89314:CDS |
| TCAGCTCCTCATGGATCTTG+AGG | 0.451104 | 7.1:+38048084 | None:intergenic |
| AGTTTAGGAGGAGGGAGAAG+TGG | 0.456414 | 7.1:-38048125 | MS.gene89314:CDS |
| CAGCTCCTCATGGATCTTGA+GGG | 0.457928 | 7.1:+38048085 | None:intergenic |
| GGATGATCAATAACTCTGCT+TGG | 0.462130 | 7.1:-38047797 | MS.gene89314:CDS |
| CTCCAACAGTGAAGGGATAT+GGG | 0.468919 | 7.1:+38047892 | None:intergenic |
| ATGTCAGAAACACTACGATT+TGG | 0.469253 | 7.1:-38048233 | MS.gene89314:CDS |
| TTCTTGTTGCAAAACTTGCA+TGG | 0.471461 | 7.1:+38048053 | None:intergenic |
| GAGTTATTGATCATCCCAAA+TGG | 0.474296 | 7.1:+38047804 | None:intergenic |
| AATTAGGAACGTTTGGACTT+GGG | 0.482030 | 7.1:-38047947 | MS.gene89314:CDS |
| TCTTGTTGCAAAACTTGCAT+GGG | 0.493245 | 7.1:+38048054 | None:intergenic |
| GGTATTCCCTCAGCTCCTCA+TGG | 0.496808 | 7.1:+38048075 | None:intergenic |
| GGCACCTTGGTGTCCTCCCA+AGG | 0.506085 | 7.1:+38048013 | None:intergenic |
| GATGATCAATAACTCTGCTT+GGG | 0.511350 | 7.1:-38047796 | MS.gene89314:CDS |
| ATTAGGAACGTTTGGACTTG+GGG | 0.514323 | 7.1:-38047946 | MS.gene89314:CDS |
| CTTGCATGGGCAACAGGTTG+AGG | 0.519092 | 7.1:+38047837 | None:intergenic |
| AGTTATTGATCATCCCAAAT+GGG | 0.522275 | 7.1:+38047805 | None:intergenic |
| ATGGGTTGTCATAGCTTGCA+TGG | 0.525223 | 7.1:+38047823 | None:intergenic |
| TTAGGAGGAGGGAGAAGTGG+AGG | 0.528661 | 7.1:-38048122 | MS.gene89314:CDS |
| ATTATTCAGTGGAGTTTGGG+TGG | 0.537735 | 7.1:+38047773 | None:intergenic |
| AACTCCACTGAATAATAATG+TGG | 0.547835 | 7.1:-38047766 | MS.gene89314:CDS |
| AAGCACTCTTTCACGCCTAT+GGG | 0.549509 | 7.1:+38047992 | None:intergenic |
| AATCCCACATTATTATTCAG+TGG | 0.569517 | 7.1:+38047762 | None:intergenic |
| TGACGACCCTCCAACAGTGA+AGG | 0.582141 | 7.1:+38047884 | None:intergenic |
| CAAGATCCATGAGGAGCTGA+GGG | 0.584633 | 7.1:-38048081 | MS.gene89314:CDS |
| TCATTGTCTCTGAGTTTAGG+AGG | 0.586488 | 7.1:-38048137 | MS.gene89314:CDS |
| GACGACCCTCCAACAGTGAA+GGG | 0.586578 | 7.1:+38047885 | None:intergenic |
| TTTCACGCCTATGGGCACCT+TGG | 0.587966 | 7.1:+38048000 | None:intergenic |
| GAGGACACCAAGGTGCCCAT+AGG | 0.592853 | 7.1:-38048007 | MS.gene89314:CDS |
| TCAAGATCCATGAGGAGCTG+AGG | 0.596229 | 7.1:-38048082 | MS.gene89314:CDS |
| TGTCTCTGAGTTTAGGAGGA+GGG | 0.598792 | 7.1:-38048133 | MS.gene89314:CDS |
| TCAAGTTCTCAAGCCTTGGG+AGG | 0.602598 | 7.1:-38048026 | MS.gene89314:CDS |
| TTGTCTCTGAGTTTAGGAGG+AGG | 0.607557 | 7.1:-38048134 | MS.gene89314:CDS |
| TGGGTTGTCATAGCTTGCAT+GGG | 0.610755 | 7.1:+38047824 | None:intergenic |
| CATATCCCTTCACTGTTGGA+GGG | 0.613445 | 7.1:-38047890 | MS.gene89314:CDS |
| ACTCCACTGAATAATAATGT+GGG | 0.629245 | 7.1:-38047765 | MS.gene89314:CDS |
| CAAGCCTTGGGAGGACACCA+AGG | 0.644910 | 7.1:-38048017 | MS.gene89314:CDS |
| CAAATCCCTCAAGATCCATG+AGG | 0.742811 | 7.1:-38048090 | MS.gene89314:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTGAAAAAGAAATTCAATT+AGG | - | chr7.1:38048019-38048038 | MS.gene89314:CDS | 15.0% |
| ! | ATTATTATTCAGTGGAGTTT+GGG | + | chr7.1:38048215-38048234 | None:intergenic | 25.0% |
| ! | TATGGGTGATAAATTGAATA+TGG | + | chr7.1:38048076-38048095 | None:intergenic | 25.0% |
| !!! | TTTAGCTTTTTGTTATCTCT+TGG | + | chr7.1:38047785-38047804 | None:intergenic | 25.0% |
| AACTCCACTGAATAATAATG+TGG | - | chr7.1:38048216-38048235 | MS.gene89314:CDS | 30.0% | |
| AATCCCACATTATTATTCAG+TGG | + | chr7.1:38048223-38048242 | None:intergenic | 30.0% | |
| ACTCCACTGAATAATAATGT+GGG | - | chr7.1:38048217-38048236 | MS.gene89314:CDS | 30.0% | |
| AGTTATTGATCATCCCAAAT+GGG | + | chr7.1:38048180-38048199 | None:intergenic | 30.0% | |
| CATTATTATTCAGTGGAGTT+TGG | + | chr7.1:38048216-38048235 | None:intergenic | 30.0% | |
| CTGAATAATAATGTGGGATT+TGG | - | chr7.1:38048223-38048242 | MS.gene89314:CDS | 30.0% | |
| GAAATTCAATTAGGAACGTT+TGG | - | chr7.1:38048028-38048047 | MS.gene89314:CDS | 30.0% | |
| AATTAGGAACGTTTGGACTT+GGG | - | chr7.1:38048035-38048054 | MS.gene89314:CDS | 35.0% | |
| ATGTCAGAAACACTACGATT+TGG | - | chr7.1:38047749-38047768 | MS.gene89314:CDS | 35.0% | |
| GAGTTATTGATCATCCCAAA+TGG | + | chr7.1:38048181-38048200 | None:intergenic | 35.0% | |
| GATGATCAATAACTCTGCTT+GGG | - | chr7.1:38048186-38048205 | MS.gene89314:CDS | 35.0% | |
| TCTTGTTGCAAAACTTGCAT+GGG | + | chr7.1:38047931-38047950 | None:intergenic | 35.0% | |
| TTCTTGTTGCAAAACTTGCA+TGG | + | chr7.1:38047932-38047951 | None:intergenic | 35.0% | |
| ! | CTTTTTATCATGGACTTGGA+TGG | - | chr7.1:38048122-38048141 | MS.gene89314:CDS | 35.0% |
| ! | TCAGCTTTTTATCATGGACT+TGG | - | chr7.1:38048118-38048137 | MS.gene89314:CDS | 35.0% |
| !!! | TTTTCAAGTTCTCAAGCCTT+GGG | - | chr7.1:38047953-38047972 | MS.gene89314:CDS | 35.0% |
| ACGTCATTGTCTCTGAGTTT+AGG | - | chr7.1:38047842-38047861 | MS.gene89314:CDS | 40.0% | |
| ATTAGGAACGTTTGGACTTG+GGG | - | chr7.1:38048036-38048055 | MS.gene89314:CDS | 40.0% | |
| ATTATTCAGTGGAGTTTGGG+TGG | + | chr7.1:38048212-38048231 | None:intergenic | 40.0% | |
| CAAAAAGCTAAAGCGCAAGA+TGG | - | chr7.1:38047793-38047812 | MS.gene89314:CDS | 40.0% | |
| CAAGCTATGACAACCCATTT+GGG | - | chr7.1:38048164-38048183 | MS.gene89314:CDS | 40.0% | |
| CAATTAGGAACGTTTGGACT+TGG | - | chr7.1:38048034-38048053 | MS.gene89314:CDS | 40.0% | |
| GGATGATCAATAACTCTGCT+TGG | - | chr7.1:38048185-38048204 | MS.gene89314:CDS | 40.0% | |
| TCATTGTCTCTGAGTTTAGG+AGG | - | chr7.1:38047845-38047864 | MS.gene89314:CDS | 40.0% | |
| !!! | GTTTTCAAGTTCTCAAGCCT+TGG | - | chr7.1:38047952-38047971 | MS.gene89314:CDS | 40.0% |
| AAGCACTCTTTCACGCCTAT+GGG | + | chr7.1:38047993-38048012 | None:intergenic | 45.0% | |
| CAAATCCCTCAAGATCCATG+AGG | - | chr7.1:38047892-38047911 | MS.gene89314:CDS | 45.0% | |
| CATATCCCTTCACTGTTGGA+GGG | - | chr7.1:38048092-38048111 | MS.gene89314:CDS | 45.0% | |
| CTCATGGATCTTGAGGGATT+TGG | + | chr7.1:38047894-38047913 | None:intergenic | 45.0% | |
| CTCCAACAGTGAAGGGATAT+GGG | + | chr7.1:38048093-38048112 | None:intergenic | 45.0% | |
| GCAAGCTATGACAACCCATT+TGG | - | chr7.1:38048163-38048182 | MS.gene89314:CDS | 45.0% | |
| TGTCTCTGAGTTTAGGAGGA+GGG | - | chr7.1:38047849-38047868 | MS.gene89314:CDS | 45.0% | |
| TTGTCTCTGAGTTTAGGAGG+AGG | - | chr7.1:38047848-38047867 | MS.gene89314:CDS | 45.0% | |
| ! | ATGGGTTGTCATAGCTTGCA+TGG | + | chr7.1:38048162-38048181 | None:intergenic | 45.0% |
| ! | GGGTCGTCAGCTTTTTATCA+TGG | - | chr7.1:38048112-38048131 | MS.gene89314:CDS | 45.0% |
| ! | TGGGTTGTCATAGCTTGCAT+GGG | + | chr7.1:38048161-38048180 | None:intergenic | 45.0% |
| AGTTTAGGAGGAGGGAGAAG+TGG | - | chr7.1:38047857-38047876 | MS.gene89314:CDS | 50.0% | |
| CAAGATCCATGAGGAGCTGA+GGG | - | chr7.1:38047901-38047920 | MS.gene89314:CDS | 50.0% | |
| CAAGCACTCTTTCACGCCTA+TGG | + | chr7.1:38047994-38048013 | None:intergenic | 50.0% | |
| CACCCATATCCCTTCACTGT+TGG | - | chr7.1:38048088-38048107 | MS.gene89314:CDS | 50.0% | |
| CAGCTCCTCATGGATCTTGA+GGG | + | chr7.1:38047900-38047919 | None:intergenic | 50.0% | |
| CCATATCCCTTCACTGTTGG+AGG | - | chr7.1:38048091-38048110 | MS.gene89314:CDS | 50.0% | |
| CCTCCAACAGTGAAGGGATA+TGG | + | chr7.1:38048094-38048113 | None:intergenic | 50.0% | |
| TCAAGATCCATGAGGAGCTG+AGG | - | chr7.1:38047900-38047919 | MS.gene89314:CDS | 50.0% | |
| TCAAGTTCTCAAGCCTTGGG+AGG | - | chr7.1:38047956-38047975 | MS.gene89314:CDS | 50.0% | |
| TCAGCTCCTCATGGATCTTG+AGG | + | chr7.1:38047901-38047920 | None:intergenic | 50.0% | |
| ! | TCATAGCTTGCATGGGCAAC+AGG | + | chr7.1:38048154-38048173 | None:intergenic | 50.0% |
| CTTGCATGGGCAACAGGTTG+AGG | + | chr7.1:38048148-38048167 | None:intergenic | 55.0% | |
| GACGACCCTCCAACAGTGAA+GGG | + | chr7.1:38048100-38048119 | None:intergenic | 55.0% | |
| GGTATTCCCTCAGCTCCTCA+TGG | + | chr7.1:38047910-38047929 | None:intergenic | 55.0% | |
| TGACGACCCTCCAACAGTGA+AGG | + | chr7.1:38048101-38048120 | None:intergenic | 55.0% | |
| TTAGGAGGAGGGAGAAGTGG+AGG | - | chr7.1:38047860-38047879 | MS.gene89314:CDS | 55.0% | |
| TTTCACGCCTATGGGCACCT+TGG | + | chr7.1:38047985-38048004 | None:intergenic | 55.0% | |
| CAAGCCTTGGGAGGACACCA+AGG | - | chr7.1:38047965-38047984 | MS.gene89314:CDS | 60.0% | |
| ! | GAGGACACCAAGGTGCCCAT+AGG | - | chr7.1:38047975-38047994 | MS.gene89314:CDS | 60.0% |
| !! | GGCACCTTGGTGTCCTCCCA+AGG | + | chr7.1:38047972-38047991 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 38047749 | 38048255 | 38047749 | ID=MS.gene89314 |
| chr7.1 | mRNA | 38047749 | 38048255 | 38047749 | ID=MS.gene89314.t1;Parent=MS.gene89314 |
| chr7.1 | exon | 38047749 | 38048255 | 38047749 | ID=MS.gene89314.t1.exon1;Parent=MS.gene89314.t1 |
| chr7.1 | CDS | 38047749 | 38048255 | 38047749 | ID=cds.MS.gene89314.t1;Parent=MS.gene89314.t1 |
| Gene Sequence |
| Protein sequence |
