Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89333.t1 | AFQ94047.1 | 91.1 | 146 | 5 | 1 | 1 | 146 | 14 | 151 | 3.30E-65 | 257.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89333.t1 | Q9SR34 | 35.6 | 177 | 70 | 6 | 1 | 139 | 1 | 171 | 5.6e-13 | 75.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89333.t1 | J7K954 | 91.1 | 146 | 5 | 1 | 1 | 146 | 14 | 151 | 2.4e-65 | 257.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene89333.t1 | TF | C2H2 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89333.t1 | MTR_7g063220 | 90.411 | 146 | 6 | 1 | 1 | 146 | 1 | 138 | 9.35e-94 | 267 |
| MS.gene89333.t1 | MTR_4g064873 | 42.208 | 154 | 57 | 6 | 24 | 146 | 21 | 173 | 2.33e-21 | 85.1 |
| MS.gene89333.t1 | MTR_1g075430 | 77.500 | 40 | 9 | 0 | 19 | 58 | 13 | 52 | 7.44e-17 | 73.6 |
| MS.gene89333.t1 | MTR_3g068095 | 68.000 | 50 | 16 | 0 | 24 | 73 | 55 | 104 | 8.61e-16 | 72.0 |
| MS.gene89333.t1 | MTR_2g076060 | 50.000 | 70 | 24 | 2 | 3 | 61 | 18 | 87 | 5.13e-15 | 69.3 |
| MS.gene89333.t1 | MTR_4g021270 | 66.667 | 42 | 14 | 0 | 24 | 65 | 59 | 100 | 1.46e-14 | 69.3 |
| MS.gene89333.t1 | MTR_5g014400 | 67.500 | 40 | 13 | 0 | 24 | 63 | 80 | 119 | 8.08e-14 | 66.6 |
| MS.gene89333.t1 | MTR_1g044225 | 65.000 | 40 | 14 | 0 | 24 | 63 | 26 | 65 | 8.54e-14 | 65.1 |
| MS.gene89333.t1 | MTR_1g070250 | 61.364 | 44 | 17 | 0 | 21 | 64 | 35 | 78 | 9.68e-14 | 65.5 |
| MS.gene89333.t1 | MTR_1g070265 | 64.286 | 42 | 13 | 1 | 15 | 56 | 28 | 67 | 1.25e-13 | 65.1 |
| MS.gene89333.t1 | MTR_5g080660 | 74.359 | 39 | 10 | 0 | 24 | 62 | 47 | 85 | 1.34e-13 | 65.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89333.t1 | AT3G09290 | 55.556 | 72 | 23 | 1 | 1 | 63 | 1 | 72 | 5.90e-19 | 79.0 |
| MS.gene89333.t1 | AT3G53820 | 34.483 | 145 | 56 | 4 | 15 | 139 | 13 | 138 | 3.84e-17 | 73.2 |
| MS.gene89333.t1 | AT2G37740 | 47.826 | 69 | 35 | 1 | 24 | 92 | 37 | 104 | 3.06e-15 | 71.2 |
| MS.gene89333.t1 | AT5G06070 | 80.556 | 36 | 7 | 0 | 24 | 59 | 53 | 88 | 5.34e-15 | 69.3 |
| MS.gene89333.t1 | AT5G43540 | 39.252 | 107 | 59 | 2 | 26 | 131 | 35 | 136 | 5.49e-14 | 65.1 |
| MS.gene89333.t1 | AT2G42410 | 55.172 | 58 | 24 | 1 | 20 | 75 | 43 | 100 | 7.41e-14 | 66.2 |
| MS.gene89333.t1 | AT2G42410 | 55.172 | 58 | 24 | 1 | 20 | 75 | 43 | 100 | 7.41e-14 | 66.2 |
| MS.gene89333.t1 | AT2G42410 | 55.172 | 58 | 24 | 1 | 20 | 75 | 69 | 126 | 1.04e-13 | 66.2 |
| MS.gene89333.t1 | AT3G23130 | 74.286 | 35 | 9 | 0 | 24 | 58 | 45 | 79 | 1.35e-13 | 65.5 |
| MS.gene89333.t1 | AT4G17810 | 36.154 | 130 | 64 | 4 | 24 | 134 | 44 | 173 | 3.54e-12 | 61.2 |
| MS.gene89333.t1 | AT4G17810 | 76.667 | 30 | 7 | 0 | 24 | 53 | 44 | 73 | 1.98e-11 | 59.7 |
Find 32 sgRNAs with CRISPR-Local
Find 35 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGAAGAAAATTTGCTTTCTT+TGG | 0.082851 | 7.3:+42152918 | MS.gene89333:CDS |
| CTTATTTGGTCAGATTTATT+AGG | 0.139528 | 7.3:-42152757 | None:intergenic |
| GGTCAGATTTATTAGGGTTT+TGG | 0.161658 | 7.3:-42152750 | None:intergenic |
| CCGAATCTGCTTGATCATTA+AGG | 0.225977 | 7.3:-42152957 | None:intergenic |
| GCTTGGCTCTATCTCTTCTA+TGG | 0.291132 | 7.3:-42152879 | None:intergenic |
| CGTCAGAGCTCCATCTTATT+TGG | 0.314582 | 7.3:-42152771 | None:intergenic |
| TTATTTGGTCAGATTTATTA+GGG | 0.342818 | 7.3:-42152756 | None:intergenic |
| GTTCTTCATTTGTTGACATA+AGG | 0.348978 | 7.3:+42153037 | MS.gene89333:CDS |
| TTCTTCATTTGTTGACATAA+GGG | 0.367087 | 7.3:+42153038 | MS.gene89333:CDS |
| AAGGGGATCCAGTGGTGTTA+TGG | 0.404055 | 7.3:+42153056 | MS.gene89333:CDS |
| CTGACGACCCAGGTCAAGTT+AGG | 0.412121 | 7.3:+42152788 | MS.gene89333:CDS |
| CCTTAATGATCAAGCAGATT+CGG | 0.423213 | 7.3:+42152957 | MS.gene89333:CDS |
| GGAAGATTGTTGTTTGAGCT+TGG | 0.430049 | 7.3:-42152896 | None:intergenic |
| TTCTCTAATGCACAAGCATT+AGG | 0.444472 | 7.3:+42152841 | MS.gene89333:CDS |
| TTAGACCTTGAGCTTAGGTT+AGG | 0.446249 | 7.3:+42153108 | MS.gene89333:CDS |
| GTGATTTAGACCTTGAGCTT+AGG | 0.467678 | 7.3:+42153103 | MS.gene89333:CDS |
| AGAGAATCATGAAACTCAAA+GGG | 0.481266 | 7.3:+42152981 | MS.gene89333:CDS |
| ATAAATCTGACCAAATAAGA+TGG | 0.504424 | 7.3:+42152761 | MS.gene89333:CDS |
| TAGGTTAGGTTATCAGCCAA+TGG | 0.520042 | 7.3:+42153122 | MS.gene89333:CDS |
| CTCTTCTATGGATATTCATG+TGG | 0.536618 | 7.3:-42152867 | None:intergenic |
| GAATAAGACCTAACTTGACC+TGG | 0.536916 | 7.3:-42152796 | None:intergenic |
| AAGAGAATCATGAAACTCAA+AGG | 0.543574 | 7.3:+42152980 | MS.gene89333:CDS |
| TCTTCATTTGTTGACATAAG+GGG | 0.545273 | 7.3:+42153039 | MS.gene89333:CDS |
| CCAGTGGTGTTATGGCTCAA+AGG | 0.569482 | 7.3:+42153064 | MS.gene89333:CDS |
| CCTTTGAGCCATAACACCAC+TGG | 0.579057 | 7.3:-42153064 | None:intergenic |
| CTCAATGTTGATGAATCCAT+TGG | 0.590079 | 7.3:-42153138 | None:intergenic |
| TCTAATGCACAAGCATTAGG+AGG | 0.629486 | 7.3:+42152844 | MS.gene89333:CDS |
| GATAACCTAACCTAAGCTCA+AGG | 0.631813 | 7.3:-42153113 | None:intergenic |
| AGATGGAGCTCTGACGACCC+AGG | 0.637866 | 7.3:+42152778 | MS.gene89333:CDS |
| AATAAGACCTAACTTGACCT+GGG | 0.655728 | 7.3:-42152795 | None:intergenic |
| GTTGACATAAGGGGATCCAG+TGG | 0.671698 | 7.3:+42153048 | MS.gene89333:CDS |
| GAGAATCATGAAACTCAAAG+GGG | 0.747840 | 7.3:+42152982 | MS.gene89333:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTATTTGGTCAGATTTATTA+GGG | - | chr7.3:42152759-42152778 | None:intergenic | 20.0% |
| ! | ATAAATCTGACCAAATAAGA+TGG | + | chr7.3:42152761-42152780 | MS.gene89333:CDS | 25.0% |
| ! | CTTATTTGGTCAGATTTATT+AGG | - | chr7.3:42152760-42152779 | None:intergenic | 25.0% |
| !! | CAAAGAAAGCAAATTTTCTT+CGG | - | chr7.3:42152920-42152939 | None:intergenic | 25.0% |
| !! | TTCTTCATTTGTTGACATAA+GGG | + | chr7.3:42153038-42153057 | MS.gene89333:CDS | 25.0% |
| AAGAGAATCATGAAACTCAA+AGG | + | chr7.3:42152980-42152999 | MS.gene89333:CDS | 30.0% | |
| AGAGAATCATGAAACTCAAA+GGG | + | chr7.3:42152981-42153000 | MS.gene89333:CDS | 30.0% | |
| ! | CGAAGAAAATTTGCTTTCTT+TGG | + | chr7.3:42152918-42152937 | MS.gene89333:CDS | 30.0% |
| ! | GTCAGATTTATTAGGGTTTT+GGG | - | chr7.3:42152752-42152771 | None:intergenic | 30.0% |
| ! | GTTCTTCATTTGTTGACATA+AGG | + | chr7.3:42153037-42153056 | MS.gene89333:CDS | 30.0% |
| ! | TCTTCATTTGTTGACATAAG+GGG | + | chr7.3:42153039-42153058 | MS.gene89333:CDS | 30.0% |
| AATAAGACCTAACTTGACCT+GGG | - | chr7.3:42152798-42152817 | None:intergenic | 35.0% | |
| CCTTAATGATCAAGCAGATT+CGG | + | chr7.3:42152957-42152976 | MS.gene89333:CDS | 35.0% | |
| CTCTTCTATGGATATTCATG+TGG | - | chr7.3:42152870-42152889 | None:intergenic | 35.0% | |
| GAGAATCATGAAACTCAAAG+GGG | + | chr7.3:42152982-42153001 | MS.gene89333:CDS | 35.0% | |
| TTCTCTAATGCACAAGCATT+AGG | + | chr7.3:42152841-42152860 | MS.gene89333:CDS | 35.0% | |
| ! | CTCAATGTTGATGAATCCAT+TGG | - | chr7.3:42153141-42153160 | None:intergenic | 35.0% |
| ! | GGTCAGATTTATTAGGGTTT+TGG | - | chr7.3:42152753-42152772 | None:intergenic | 35.0% |
| !! | TCTTGTACTTTTTGCAAGAG+AGG | + | chr7.3:42152817-42152836 | MS.gene89333:CDS | 35.0% |
| CCGAATCTGCTTGATCATTA+AGG | - | chr7.3:42152960-42152979 | None:intergenic | 40.0% | |
| GAATAAGACCTAACTTGACC+TGG | - | chr7.3:42152799-42152818 | None:intergenic | 40.0% | |
| GATAACCTAACCTAAGCTCA+AGG | - | chr7.3:42153116-42153135 | None:intergenic | 40.0% | |
| GTGATTTAGACCTTGAGCTT+AGG | + | chr7.3:42153103-42153122 | MS.gene89333:CDS | 40.0% | |
| TAGGTTAGGTTATCAGCCAA+TGG | + | chr7.3:42153122-42153141 | MS.gene89333:CDS | 40.0% | |
| TCTAATGCACAAGCATTAGG+AGG | + | chr7.3:42152844-42152863 | MS.gene89333:CDS | 40.0% | |
| TTAGACCTTGAGCTTAGGTT+AGG | + | chr7.3:42153108-42153127 | MS.gene89333:CDS | 40.0% | |
| !! | GGAAGATTGTTGTTTGAGCT+TGG | - | chr7.3:42152899-42152918 | None:intergenic | 40.0% |
| CGTCAGAGCTCCATCTTATT+TGG | - | chr7.3:42152774-42152793 | None:intergenic | 45.0% | |
| !! | GCTTGGCTCTATCTCTTCTA+TGG | - | chr7.3:42152882-42152901 | None:intergenic | 45.0% |
| CCTTTGAGCCATAACACCAC+TGG | - | chr7.3:42153067-42153086 | None:intergenic | 50.0% | |
| GTTGACATAAGGGGATCCAG+TGG | + | chr7.3:42153048-42153067 | MS.gene89333:CDS | 50.0% | |
| ! | AAGGGGATCCAGTGGTGTTA+TGG | + | chr7.3:42153056-42153075 | MS.gene89333:CDS | 50.0% |
| !! | CCAGTGGTGTTATGGCTCAA+AGG | + | chr7.3:42153064-42153083 | MS.gene89333:CDS | 50.0% |
| CTGACGACCCAGGTCAAGTT+AGG | + | chr7.3:42152788-42152807 | MS.gene89333:CDS | 55.0% | |
| ! | AGATGGAGCTCTGACGACCC+AGG | + | chr7.3:42152778-42152797 | MS.gene89333:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 42152739 | 42153179 | 42152739 | ID=MS.gene89333 |
| chr7.3 | mRNA | 42152739 | 42153179 | 42152739 | ID=MS.gene89333.t1;Parent=MS.gene89333 |
| chr7.3 | exon | 42152739 | 42153179 | 42152739 | ID=MS.gene89333.t1.exon1;Parent=MS.gene89333.t1 |
| chr7.3 | CDS | 42152739 | 42153179 | 42152739 | ID=cds.MS.gene89333.t1;Parent=MS.gene89333.t1 |
| Gene Sequence |
| Protein sequence |
