AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene89498

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene89498.t1 MTR_5g062970 81.532 222 18 1 52 250 8 229 2.85e-127 362
MS.gene89498.t1 MTR_1g058210 54.065 246 71 5 82 288 12 254 3.22e-75 237
MS.gene89498.t1 MTR_5g061220 46.863 271 79 4 79 288 16 282 8.02e-71 228
MS.gene89498.t1 MTR_2g024430 49.793 241 84 8 82 288 20 257 2.11e-62 206
MS.gene89498.t1 MTR_5g060630 47.273 275 78 8 79 288 9 281 1.04e-59 199
MS.gene89498.t1 MTR_5g460740 47.273 275 78 8 79 288 9 281 1.04e-59 199
MS.gene89498.t1 MTR_5g040880 44.813 241 101 4 79 288 8 247 1.75e-59 198
MS.gene89498.t1 MTR_5g040740 47.303 241 91 5 82 288 12 250 3.92e-59 194
MS.gene89498.t1 MTR_5g060780 47.368 247 77 8 82 288 12 245 5.16e-59 194
MS.gene89498.t1 MTR_5g460920 47.368 247 77 8 82 288 12 245 2.23e-58 192
MS.gene89498.t1 MTR_5g061890 47.773 247 76 8 82 288 12 245 3.81e-58 191
MS.gene89498.t1 MTR_2g006270 43.852 244 100 5 82 288 13 256 1.51e-51 176
MS.gene89498.t1 MTR_5g082140 43.568 241 101 6 82 288 12 251 5.00e-50 172
MS.gene89498.t1 MTR_2g094570 34.657 277 111 4 82 288 11 287 7.90e-46 164
MS.gene89498.t1 MTR_2g094570 34.164 281 115 4 78 288 7 287 8.80e-46 164
MS.gene89498.t1 MTR_2g094570 34.657 277 111 4 82 288 67 343 1.40e-45 164
MS.gene89498.t1 MTR_2g006380 39.918 243 106 7 82 288 20 258 1.08e-44 157
MS.gene89498.t1 MTR_3g073420 41.063 207 101 2 74 259 11 217 2.02e-43 156
MS.gene89498.t1 MTR_1g094960 34.875 281 110 7 79 288 16 294 3.19e-41 150
MS.gene89498.t1 MTR_4g058930 41.500 200 93 2 82 259 12 209 4.92e-40 148
MS.gene89498.t1 MTR_4g058930 41.500 200 93 2 82 259 12 209 5.74e-40 148
MS.gene89498.t1 MTR_1g064430 32.281 285 115 4 82 288 40 324 3.05e-39 146
MS.gene89498.t1 MTR_1g064430 32.281 285 115 4 82 288 27 311 3.45e-39 145
MS.gene89498.t1 MTR_7g101275 34.335 233 117 3 92 288 2 234 1.79e-38 140
MS.gene89498.t1 MTR_7g101280 33.654 208 109 4 78 259 27 231 1.04e-33 130
MS.gene89498.t1 MTR_0041s0110 33.654 208 109 4 78 259 27 231 1.04e-33 130
MS.gene89498.t1 MTR_2g014770 31.890 254 128 4 78 288 49 300 1.62e-33 129
MS.gene89498.t1 MTR_2g014770 31.890 254 128 4 78 288 49 300 2.25e-33 129
MS.gene89498.t1 MTR_2g005240 35.961 203 105 4 82 259 23 225 1.36e-31 124
MS.gene89498.t1 MTR_7g062540 30.769 247 134 4 82 291 14 260 2.21e-31 123
MS.gene89498.t1 MTR_8g100050 34.314 204 108 3 82 259 59 262 2.64e-31 123
MS.gene89498.t1 MTR_8g100050 34.314 204 108 3 82 259 59 262 3.20e-31 123
MS.gene89498.t1 MTR_0041s0120 31.707 246 118 5 92 288 2 246 3.48e-31 120
MS.gene89498.t1 MTR_8g027440 34.975 203 107 3 82 259 29 231 3.65e-31 123
MS.gene89498.t1 MTR_2g018690 32.479 234 124 4 81 285 22 250 4.44e-31 123
MS.gene89498.t1 MTR_8g079492 33.971 209 108 3 81 261 22 228 1.23e-30 121
MS.gene89498.t1 MTR_8g079492 33.971 209 108 3 81 261 22 228 1.24e-30 121
MS.gene89498.t1 MTR_4g021580 33.990 203 109 3 82 259 19 221 1.90e-30 120
MS.gene89498.t1 MTR_4g021580 33.990 203 109 3 82 259 19 221 2.00e-30 120
MS.gene89498.t1 MTR_4g088210 32.558 215 108 4 82 261 38 250 1.31e-29 118
MS.gene89498.t1 MTR_4g088210 32.558 215 108 4 82 261 38 250 1.51e-29 118
MS.gene89498.t1 MTR_2g043250 32.365 241 117 6 53 261 2 228 1.54e-29 118
MS.gene89498.t1 MTR_1g024025 32.710 214 118 4 78 265 39 252 2.48e-29 118
MS.gene89498.t1 MTR_5g076270 32.479 234 124 4 81 285 23 251 5.41e-29 117
MS.gene89498.t1 MTR_4g124900 32.243 214 107 5 78 259 19 226 2.43e-28 115
MS.gene89498.t1 MTR_4g124900 32.243 214 107 5 78 259 19 226 2.90e-28 114
MS.gene89498.t1 MTR_3g064050 31.624 234 126 4 81 285 25 253 3.02e-28 114
MS.gene89498.t1 MTR_2g093740 31.925 213 110 4 82 259 56 268 1.62e-27 112
MS.gene89498.t1 MTR_2g093740 31.628 215 112 4 82 261 56 270 1.72e-27 112
MS.gene89498.t1 MTR_2g093740 31.628 215 112 4 82 261 56 270 1.83e-27 112
MS.gene89498.t1 MTR_8g101360 29.439 214 124 2 79 265 28 241 4.59e-27 111
MS.gene89498.t1 MTR_2g093740 29.825 228 112 5 82 261 56 283 2.33e-24 103
MS.gene89498.t1 MTR_2g093740 29.825 228 112 5 82 261 56 283 2.69e-24 103
MS.gene89498.t1 MTR_2g093740 29.825 228 112 5 82 261 56 283 2.80e-24 103
MS.gene89498.t1 MTR_4g060460 30.516 213 113 2 82 259 54 266 8.62e-24 101
MS.gene89498.t1 MTR_4g060460 30.516 213 113 2 82 259 54 266 8.64e-24 101
MS.gene89498.t1 MTR_5g060770 42.857 140 53 4 149 288 50 162 7.98e-23 97.1
MS.gene89498.t1 MTR_8g079492 36.957 138 85 1 124 261 11 146 1.20e-22 98.2
MS.gene89498.t1 MTR_2g093740 36.508 126 73 1 141 259 13 138 4.44e-20 90.5
MS.gene89498.t1 MTR_2g093740 36.508 126 73 1 141 259 13 138 4.65e-20 90.5
MS.gene89498.t1 MTR_8g446900 31.757 148 86 2 156 288 59 206 3.20e-17 81.3
MS.gene89498.t1 MTR_2g093740 33.094 139 73 2 141 259 13 151 6.13e-17 81.3
MS.gene89498.t1 MTR_2g093740 33.094 139 73 2 141 259 13 151 6.64e-17 81.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene89498.t1 AT1G77850 38.406 276 95 7 83 288 20 290 4.98e-49 172
MS.gene89498.t1 AT1G77850 38.406 276 95 7 83 288 20 290 5.32e-49 171
MS.gene89498.t1 AT4G30080 33.333 279 112 6 82 288 19 295 7.56e-42 153
MS.gene89498.t1 AT2G28350 36.052 233 92 4 82 271 10 228 6.28e-39 145
MS.gene89498.t1 AT2G28350 36.052 233 92 4 82 271 10 228 8.26e-39 144
MS.gene89498.t1 AT2G46530 33.333 231 125 4 81 285 41 268 1.74e-34 132
MS.gene89498.t1 AT2G46530 33.333 231 125 4 81 285 20 247 1.79e-34 132
MS.gene89498.t1 AT3G61830 35.122 205 107 3 81 259 24 228 6.86e-34 130
MS.gene89498.t1 AT4G23980 34.634 205 107 3 82 259 12 216 9.53e-33 127
MS.gene89498.t1 AT4G23980 34.634 205 107 3 82 259 12 216 1.09e-32 127
MS.gene89498.t1 AT2G33860 33.484 221 114 5 82 272 54 271 3.79e-32 125
MS.gene89498.t1 AT1G34310 38.000 200 102 4 82 259 27 226 7.33e-32 124
MS.gene89498.t1 AT5G62000 33.333 204 110 3 82 259 61 264 6.23e-31 122
MS.gene89498.t1 AT5G62000 33.333 204 110 3 82 259 61 264 7.14e-31 122
MS.gene89498.t1 AT5G62000 33.333 204 110 3 82 259 61 264 7.14e-31 122
MS.gene89498.t1 AT5G62000 33.333 204 110 3 82 259 61 264 7.14e-31 122
MS.gene89498.t1 AT5G62000 33.333 204 110 3 82 259 61 264 7.14e-31 122
MS.gene89498.t1 AT1G35540 35.468 203 108 4 82 262 27 228 1.10e-30 121
MS.gene89498.t1 AT1G34390 34.483 203 108 6 82 262 27 226 1.40e-30 121
MS.gene89498.t1 AT5G37020 33.981 206 105 3 81 257 21 224 2.70e-29 117
MS.gene89498.t1 AT5G37020 33.981 206 105 3 81 257 21 224 3.22e-29 117
MS.gene89498.t1 AT1G30330 31.624 234 126 4 81 285 24 252 3.76e-29 117
MS.gene89498.t1 AT1G30330 31.624 234 126 4 81 285 24 252 3.76e-29 117
MS.gene89498.t1 AT1G30330 31.624 234 126 4 81 285 22 250 4.01e-29 117
MS.gene89498.t1 AT1G59750 31.553 206 110 3 82 259 22 224 5.20e-29 116
MS.gene89498.t1 AT1G59750 31.553 206 110 3 82 259 22 224 5.48e-29 116
MS.gene89498.t1 AT1G59750 31.553 206 110 3 82 259 22 224 5.48e-29 116
MS.gene89498.t1 AT1G59750 31.553 206 110 3 82 259 22 224 5.92e-29 116
MS.gene89498.t1 AT1G35240 36.500 200 105 4 82 259 27 226 1.34e-28 115
MS.gene89498.t1 AT1G35520 36.500 200 105 4 82 259 27 226 2.00e-28 114
MS.gene89498.t1 AT1G19850 31.356 236 133 4 57 265 31 264 2.67e-28 115
MS.gene89498.t1 AT1G19850 31.356 236 133 4 57 265 20 253 2.93e-28 114
MS.gene89498.t1 AT1G19220 33.803 213 103 5 79 259 20 226 5.85e-28 114
MS.gene89498.t1 AT1G34410 35.500 200 107 4 82 259 27 226 1.80e-27 112
MS.gene89498.t1 AT5G20730 33.023 215 104 6 78 259 20 227 3.84e-27 111
MS.gene89498.t1 AT5G20730 33.023 215 104 6 78 259 20 227 4.44e-27 111
MS.gene89498.t1 AT5G20730 33.023 215 104 6 78 259 20 227 4.52e-27 111
MS.gene89498.t1 AT1G43950 35.000 200 101 7 82 257 27 221 4.72e-25 100
MS.gene89498.t1 AT5G60450 30.667 225 115 5 75 261 34 255 4.91e-25 105
MS.gene89498.t1 AT5G60450 30.769 221 112 5 79 261 62 279 1.12e-24 104
MS.gene89498.t1 AT2G46530 38.168 131 78 1 155 285 33 160 1.17e-23 100
MS.gene89498.t1 AT3G61830 43.137 102 58 0 158 259 76 177 6.69e-23 99.0
MS.gene89498.t1 AT2G33860 39.474 114 68 1 159 272 2 114 2.04e-22 97.1
MS.gene89498.t1 AT1G34170 28.148 270 151 8 60 288 1 268 3.38e-22 96.7
MS.gene89498.t1 AT1G34170 28.148 270 151 8 60 288 1 268 4.03e-22 96.7
MS.gene89498.t1 AT1G34170 28.148 270 151 8 60 288 1 268 5.00e-22 96.3
MS.gene89498.t1 AT5G37020 41.414 99 58 0 159 257 31 129 2.02e-20 91.7

Find 70 sgRNAs with CRISPR-Local

Find 98 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGGAATCTTCGAGATTTC+AGG 0.221871 5.4:+49145190 None:intergenic
TCGTCTTCGGCAAGAACTTC+TGG 0.244689 5.4:+49144812 None:intergenic
AGCACATTTCTGCCAGATTT+TGG 0.250965 5.4:+49145000 None:intergenic
CTGGAGGAGCTTCTTGTTCA+TGG 0.266288 5.4:+49144831 None:intergenic
AAGTCCATCCCCTACTAAAA+AGG 0.312107 5.4:+49144576 None:intergenic
TGGCCATGGCCATCCGTTAC+AGG 0.326930 5.4:+49144851 None:intergenic
TGCCAAGGATTGACACAGTT+TGG 0.353376 5.4:+49145172 None:intergenic
GGTAACAGAGAAGGCGGTTA+TGG 0.361245 5.4:-49144453 MS.gene89498:CDS
GGTTATGGATGCGGTGGAAT+TGG 0.365128 5.4:-49144438 MS.gene89498:CDS
ACACAATCTGATTGCAATAG+TGG 0.393698 5.4:-49144752 MS.gene89498:CDS
GCTCCCCGGTATCACTTAAC+AGG 0.396579 5.4:+49145068 None:intergenic
CTGACATTCAAGGCCGAGTC+TGG 0.403843 5.4:-49144637 MS.gene89498:CDS
GTATATACAGTTCAGCTTTG+TGG 0.420575 5.4:+49145134 None:intergenic
CATTATCTGATGAATTCTTA+AGG 0.441975 5.4:+49145512 None:intergenic
GATCTTGCCGAAGCTTAACT+TGG 0.446257 5.4:-49144693 MS.gene89498:CDS
TTACCATCCCTACCAAAATC+TGG 0.452282 5.4:-49145012 MS.gene89498:CDS
CTCTCCGTCACTGACATTCA+AGG 0.461163 5.4:-49144647 MS.gene89498:CDS
CTGATGAATTCTTAAGGCAC+AGG 0.466981 5.4:+49145518 None:intergenic
TCTGTCCTGTTAAGTGATAC+CGG 0.467467 5.4:-49145073 MS.gene89498:CDS
TCTGATTGCAATAGTGGCTA+TGG 0.469763 5.4:-49144746 MS.gene89498:CDS
ATTGTAGCCATCCTCGTCTT+CGG 0.478792 5.4:+49144799 None:intergenic
GAATCGTCCAAGTTAAGCTT+CGG 0.478925 5.4:+49144686 None:intergenic
TAAACTTTAGAATGTAGCTT+CGG 0.481122 5.4:+49144959 None:intergenic
TTCCAAACTGTGTCAATCCT+TGG 0.497383 5.4:-49145174 MS.gene89498:CDS
AAAATCTGGCAGAAATGTGC+TGG 0.507785 5.4:-49144998 MS.gene89498:CDS
CTGTCCTGTTAAGTGATACC+GGG 0.511775 5.4:-49145072 MS.gene89498:CDS
AAACTGTGTCAATCCTTGGC+AGG 0.512278 5.4:-49145170 MS.gene89498:CDS
AGGAGGATAACGATGAAGAA+GGG 0.521368 5.4:+49144886 None:intergenic
AAGGAGGATAACGATGAAGA+AGG 0.529163 5.4:+49144885 None:intergenic
AGAGAAGGCGGTTATGGATG+CGG 0.535241 5.4:-49144447 MS.gene89498:CDS
GAGCTTCTTGTTCATGGCCA+TGG 0.538260 5.4:+49144837 None:intergenic
GTTGGAATATGCCGGAAAAG+TGG 0.542178 5.4:-49144479 MS.gene89498:CDS
TTCCATCCTCACTCCTGTAA+CGG 0.546200 5.4:-49144864 MS.gene89498:CDS
TATGGATGCGGTGGAATTGG+CGG 0.548189 5.4:-49144435 MS.gene89498:CDS
ATCCGTTACAGGAGTGAGGA+TGG 0.551125 5.4:+49144862 None:intergenic
ACAGAGCTCTCAGCACGTCT+AGG 0.552883 5.4:+49145091 None:intergenic
AGATATTCGTTGGAATATGC+CGG 0.558862 5.4:-49144487 MS.gene89498:CDS
TCAGCTGGGAAGATATTCGT+TGG 0.559165 5.4:-49144497 MS.gene89498:CDS
AACAACAAGAAACTCGTCGC+TGG 0.566110 5.4:-49144545 MS.gene89498:CDS
GGCAAGATCAAGTCAACGCA+AGG 0.570350 5.4:+49144707 None:intergenic
CATGTTCTAAGTGACCTTGT+GGG 0.576431 5.4:+49144930 None:intergenic
TTGGAATATGCCGGAAAAGT+GGG 0.577884 5.4:-49144478 MS.gene89498:CDS
ATCATCAACAACATTTGCAT+CGG 0.582794 5.4:+49144380 None:intergenic
ACATGTTCTAAGTGACCTTG+TGG 0.589249 5.4:+49144929 None:intergenic
TGCTTCGGAATGTAGCTCCC+CGG 0.594410 5.4:+49145054 None:intergenic
TATGCGTGTACTGCCAGACT+CGG 0.597780 5.4:+49144624 None:intergenic
ATCCTCACTCCTGTAACGGA+TGG 0.600039 5.4:-49144860 MS.gene89498:CDS
ACTCCTGTAACGGATGGCCA+TGG 0.603479 5.4:-49144854 MS.gene89498:CDS
TGAATAAGTTCAACCTGCCA+AGG 0.607758 5.4:+49145157 None:intergenic
GTTACAGGAGTGAGGATGGA+AGG 0.609129 5.4:+49144866 None:intergenic
AAGGGTGATGATTGTGTGAG+CGG 0.624730 5.4:+49144904 None:intergenic
AGAAGTTCTTGCCGAAGACG+AGG 0.627152 5.4:-49144810 MS.gene89498:CDS
AAGTGGGAAGGTAACAGAGA+AGG 0.628618 5.4:-49144462 MS.gene89498:CDS
GTTCTTGCCGAAGACGAGGA+TGG 0.629556 5.4:-49144806 MS.gene89498:CDS
GAAGGCGGTTATGGATGCGG+TGG 0.634390 5.4:-49144444 MS.gene89498:CDS
GGCCATCCGTTACAGGAGTG+AGG 0.635015 5.4:+49144858 None:intergenic
TGATGAATTCTTAAGGCACA+GGG 0.640047 5.4:+49145519 None:intergenic
TTGTGTGAGAATCTTGACAA+AGG 0.644198 5.4:+49144769 None:intergenic
ACAGGAGTGAGGATGGAAGG+AGG 0.648192 5.4:+49144869 None:intergenic
GTGACGGAGAGTGTCTGAGA+TGG 0.649352 5.4:+49144659 None:intergenic
AAAGTTTACTACTTCCCACA+AGG 0.649931 5.4:-49144944 MS.gene89498:CDS
TCTTCGGCAAGAACTTCTGG+AGG 0.650043 5.4:+49144815 None:intergenic
TCGGCCTTGAATGTCAGTGA+CGG 0.654538 5.4:+49144643 None:intergenic
GCAAGATCAAGTCAACGCAA+GGG 0.677166 5.4:+49144708 None:intergenic
TGGGAAGGTAACAGAGAAGG+CGG 0.684452 5.4:-49144459 MS.gene89498:CDS
GGTGTCTCTTGGATTTACCG+CGG 0.707333 5.4:+49144597 None:intergenic
AATATGCCGGAAAAGTGGGA+AGG 0.715565 5.4:-49144474 MS.gene89498:CDS
TGTCCTGTTAAGTGATACCG+GGG 0.717824 5.4:-49145071 MS.gene89498:CDS
TAGCTTCGGAATCTTGACTG+AGG 0.723506 5.4:+49144973 None:intergenic
CAGTACACGCATATCTACCG+CGG 0.778287 5.4:-49144614 MS.gene89498:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATCAAAAGATATAAAAAAAA+TGG + chr5.4:49144669-49144688 None:intergenic 10.0%
!! TTCTTAGAAAATATCACTAT+TGG + chr5.4:49144491-49144510 None:intergenic 20.0%
!!! ATGGTAAATATAGATTTTGT+GGG + chr5.4:49144873-49144892 None:intergenic 20.0%
! CATTATCTGATGAATTCTTA+AGG + chr5.4:49144389-49144408 None:intergenic 25.0%
!! ATCTTTTGATCATAGTTAAC+TGG - chr5.4:49144679-49144698 MS.gene89498:CDS 25.0%
!! GATGGTAAATATAGATTTTG+TGG + chr5.4:49144874-49144893 None:intergenic 25.0%
!! TCTTTTGATCATAGTTAACT+GGG - chr5.4:49144680-49144699 MS.gene89498:CDS 25.0%
!! TGGTAAATATAGATTTTGTG+GGG + chr5.4:49144872-49144891 None:intergenic 25.0%
!!! TAAACTTTAGAATGTAGCTT+CGG + chr5.4:49144942-49144961 None:intergenic 25.0%
ATCATCAACAACATTTGCAT+CGG + chr5.4:49145521-49145540 None:intergenic 30.0%
CTATTTCAAATGACTGATGT+TGG - chr5.4:49144636-49144655 MS.gene89498:CDS 30.0%
! TTTTCGTTAAAAACTCAGCT+GGG - chr5.4:49145387-49145406 MS.gene89498:intron 30.0%
!! ACGTCTAGGATTTTTATTTG+GGG + chr5.4:49144796-49144815 None:intergenic 30.0%
!! CACGTCTAGGATTTTTATTT+GGG + chr5.4:49144797-49144816 None:intergenic 30.0%
AAAGTTTACTACTTCCCACA+AGG - chr5.4:49144954-49144973 MS.gene89498:CDS 35.0%
ACACAATCTGATTGCAATAG+TGG - chr5.4:49145146-49145165 MS.gene89498:CDS 35.0%
AGATATTCGTTGGAATATGC+CGG - chr5.4:49145411-49145430 MS.gene89498:intron 35.0%
GTATATACAGTTCAGCTTTG+TGG + chr5.4:49144767-49144786 None:intergenic 35.0%
TGATGAATTCTTAAGGCACA+GGG + chr5.4:49144382-49144401 None:intergenic 35.0%
TTGTGTGAGAATCTTGACAA+AGG + chr5.4:49145132-49145151 None:intergenic 35.0%
! GTTTTCGTTAAAAACTCAGC+TGG - chr5.4:49145386-49145405 MS.gene89498:intron 35.0%
! TTTGGAATCTTCGAGATTTC+AGG + chr5.4:49144711-49144730 None:intergenic 35.0%
!! AAGAGACACCTTTTTAGTAG+GGG - chr5.4:49145314-49145333 MS.gene89498:intron 35.0%
!! ATTTCTGCCAGATTTTGGTA+GGG + chr5.4:49144896-49144915 None:intergenic 35.0%
!! CAAGAGACACCTTTTTAGTA+GGG - chr5.4:49145313-49145332 MS.gene89498:intron 35.0%
!! CGTCTAGGATTTTTATTTGG+GGG + chr5.4:49144795-49144814 None:intergenic 35.0%
!! GCACGTCTAGGATTTTTATT+TGG + chr5.4:49144798-49144817 None:intergenic 35.0%
!! GTCTAGGATTTTTATTTGGG+GGG + chr5.4:49144794-49144813 None:intergenic 35.0%
!! TCTAGGATTTTTATTTGGGG+GGG + chr5.4:49144793-49144812 None:intergenic 35.0%
AAAATCTGGCAGAAATGTGC+TGG - chr5.4:49144900-49144919 MS.gene89498:CDS 40.0%
AAGGAGGATAACGATGAAGA+AGG + chr5.4:49145016-49145035 None:intergenic 40.0%
AAGTCCATCCCCTACTAAAA+AGG + chr5.4:49145325-49145344 None:intergenic 40.0%
ACTATTGGAGCAAGTTACGT+AGG + chr5.4:49144476-49144495 None:intergenic 40.0%
AGGAGGATAACGATGAAGAA+GGG + chr5.4:49145015-49145034 None:intergenic 40.0%
CTGATGAATTCTTAAGGCAC+AGG + chr5.4:49144383-49144402 None:intergenic 40.0%
GAATCGTCCAAGTTAAGCTT+CGG + chr5.4:49145215-49145234 None:intergenic 40.0%
TCTGATTGCAATAGTGGCTA+TGG - chr5.4:49145152-49145171 MS.gene89498:CDS 40.0%
TCTGTCCTGTTAAGTGATAC+CGG - chr5.4:49144825-49144844 MS.gene89498:CDS 40.0%
TGAATAAGTTCAACCTGCCA+AGG + chr5.4:49144744-49144763 None:intergenic 40.0%
TTACCATCCCTACCAAAATC+TGG - chr5.4:49144886-49144905 MS.gene89498:CDS 40.0%
TTCCAAACTGTGTCAATCCT+TGG - chr5.4:49144724-49144743 MS.gene89498:CDS 40.0%
TTGGAATATGCCGGAAAAGT+GGG - chr5.4:49145420-49145439 MS.gene89498:intron 40.0%
! ACATGTTCTAAGTGACCTTG+TGG + chr5.4:49144972-49144991 None:intergenic 40.0%
! AGATTTTGTGGGGAATGCTT+CGG + chr5.4:49144862-49144881 None:intergenic 40.0%
! AGCACATTTCTGCCAGATTT+TGG + chr5.4:49144901-49144920 None:intergenic 40.0%
! CATGTTCTAAGTGACCTTGT+GGG + chr5.4:49144971-49144990 None:intergenic 40.0%
!! CATTTCTGCCAGATTTTGGT+AGG + chr5.4:49144897-49144916 None:intergenic 40.0%
!! CCAAGAGACACCTTTTTAGT+AGG - chr5.4:49145312-49145331 MS.gene89498:intron 40.0%
!! CCTACTAAAAAGGTGTCTCT+TGG + chr5.4:49145315-49145334 None:intergenic 40.0%
AAACTGTGTCAATCCTTGGC+AGG - chr5.4:49144728-49144747 MS.gene89498:CDS 45.0%
AACAACAAGAAACTCGTCGC+TGG - chr5.4:49145353-49145372 MS.gene89498:intron 45.0%
AAGGGTGATGATTGTGTGAG+CGG + chr5.4:49144997-49145016 None:intergenic 45.0%
AAGTGGGAAGGTAACAGAGA+AGG - chr5.4:49145436-49145455 MS.gene89498:intron 45.0%
AATATGCCGGAAAAGTGGGA+AGG - chr5.4:49145424-49145443 MS.gene89498:intron 45.0%
ATTGTAGCCATCCTCGTCTT+CGG + chr5.4:49145102-49145121 None:intergenic 45.0%
CTGTCCTGTTAAGTGATACC+GGG - chr5.4:49144826-49144845 MS.gene89498:CDS 45.0%
GATCTTGCCGAAGCTTAACT+TGG - chr5.4:49145205-49145224 MS.gene89498:CDS 45.0%
GCAAGATCAAGTCAACGCAA+GGG + chr5.4:49145193-49145212 None:intergenic 45.0%
GGAATTGGCGGTGAAAAACA+TGG - chr5.4:49145475-49145494 MS.gene89498:intron 45.0%
GTTGGAATATGCCGGAAAAG+TGG - chr5.4:49145419-49145438 MS.gene89498:intron 45.0%
TAGCTTCGGAATCTTGACTG+AGG + chr5.4:49144928-49144947 None:intergenic 45.0%
TCAGCTGGGAAGATATTCGT+TGG - chr5.4:49145401-49145420 MS.gene89498:intron 45.0%
TGCCAAGGATTGACACAGTT+TGG + chr5.4:49144729-49144748 None:intergenic 45.0%
TGTCCTGTTAAGTGATACCG+GGG - chr5.4:49144827-49144846 MS.gene89498:CDS 45.0%
TTCCATCCTCACTCCTGTAA+CGG - chr5.4:49145034-49145053 MS.gene89498:CDS 45.0%
!! CTGTTACCTTCCCACTTTTC+CGG + chr5.4:49145433-49145452 None:intergenic 45.0%
!! GACACCTTTTTAGTAGGGGA+TGG - chr5.4:49145318-49145337 MS.gene89498:intron 45.0%
AGAAGTTCTTGCCGAAGACG+AGG - chr5.4:49145088-49145107 MS.gene89498:CDS 50.0%
AGAGAAGGCGGTTATGGATG+CGG - chr5.4:49145451-49145470 MS.gene89498:intron 50.0%
ATCCGTTACAGGAGTGAGGA+TGG + chr5.4:49145039-49145058 None:intergenic 50.0%
CAGTACACGCATATCTACCG+CGG - chr5.4:49145284-49145303 MS.gene89498:intron 50.0%
CTCTCCGTCACTGACATTCA+AGG - chr5.4:49145251-49145270 MS.gene89498:intron 50.0%
CTGGAGGAGCTTCTTGTTCA+TGG + chr5.4:49145070-49145089 None:intergenic 50.0%
GAGCTTCTTGTTCATGGCCA+TGG + chr5.4:49145064-49145083 None:intergenic 50.0%
GGCAAGATCAAGTCAACGCA+AGG + chr5.4:49145194-49145213 None:intergenic 50.0%
GGTAACAGAGAAGGCGGTTA+TGG - chr5.4:49145445-49145464 MS.gene89498:intron 50.0%
GGTGTCTCTTGGATTTACCG+CGG + chr5.4:49145304-49145323 None:intergenic 50.0%
GGTTATGGATGCGGTGGAAT+TGG - chr5.4:49145460-49145479 MS.gene89498:intron 50.0%
GTTACAGGAGTGAGGATGGA+AGG + chr5.4:49145035-49145054 None:intergenic 50.0%
TATGCGTGTACTGCCAGACT+CGG + chr5.4:49145277-49145296 None:intergenic 50.0%
TATGGATGCGGTGGAATTGG+CGG - chr5.4:49145463-49145482 MS.gene89498:intron 50.0%
TCGGCCTTGAATGTCAGTGA+CGG + chr5.4:49145258-49145277 None:intergenic 50.0%
TCGTCTTCGGCAAGAACTTC+TGG + chr5.4:49145089-49145108 None:intergenic 50.0%
TCTTCGGCAAGAACTTCTGG+AGG + chr5.4:49145086-49145105 None:intergenic 50.0%
TGGGAAGGTAACAGAGAAGG+CGG - chr5.4:49145439-49145458 MS.gene89498:intron 50.0%
! ATCCTCACTCCTGTAACGGA+TGG - chr5.4:49145038-49145057 MS.gene89498:CDS 50.0%
!! CTGCCAGATTTTGGTAGGGA+TGG + chr5.4:49144892-49144911 None:intergenic 50.0%
ACAGAGCTCTCAGCACGTCT+AGG + chr5.4:49144810-49144829 None:intergenic 55.0%
ACAGGAGTGAGGATGGAAGG+AGG + chr5.4:49145032-49145051 None:intergenic 55.0%
CTGACATTCAAGGCCGAGTC+TGG - chr5.4:49145261-49145280 MS.gene89498:intron 55.0%
GCTCCCCGGTATCACTTAAC+AGG + chr5.4:49144833-49144852 None:intergenic 55.0%
GTTCTTGCCGAAGACGAGGA+TGG - chr5.4:49145092-49145111 MS.gene89498:CDS 55.0%
TGCTTCGGAATGTAGCTCCC+CGG + chr5.4:49144847-49144866 None:intergenic 55.0%
!! ACTCCTGTAACGGATGGCCA+TGG - chr5.4:49145044-49145063 MS.gene89498:CDS 55.0%
!! GTGACGGAGAGTGTCTGAGA+TGG + chr5.4:49145242-49145261 None:intergenic 55.0%
GAAGGCGGTTATGGATGCGG+TGG - chr5.4:49145454-49145473 MS.gene89498:intron 60.0%
GGCCATCCGTTACAGGAGTG+AGG + chr5.4:49145043-49145062 None:intergenic 60.0%
TGGCCATGGCCATCCGTTAC+AGG + chr5.4:49145050-49145069 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 49144373 49145547 49144373 ID=MS.gene89498
chr5.4 mRNA 49144373 49145547 49144373 ID=MS.gene89498.t1;Parent=MS.gene89498
chr5.4 exon 49145511 49145547 49145511 ID=MS.gene89498.t1.exon1;Parent=MS.gene89498.t1
chr5.4 CDS 49145511 49145547 49145511 ID=cds.MS.gene89498.t1;Parent=MS.gene89498.t1
chr5.4 exon 49144373 49145226 49144373 ID=MS.gene89498.t1.exon2;Parent=MS.gene89498.t1
chr5.4 CDS 49144373 49145226 49144373 ID=cds.MS.gene89498.t1;Parent=MS.gene89498.t1
Gene Sequence

>MS.gene89498

ATGGTTCCCTGTGCCTTAAGAATTCATCAGATAATGTGTATGTAAGCAGATTACATTTTACAATCAATTGTTTATGTTAGACTTTTTTTATATGTCACTGCCTACGTAACTTGCTCCAATAGTGATATTTTCTAAGAAACAGTTATGAAGTTTAAAAACTTTCTAATTATTTCTCGTTGTAAATATTCTTAATGCTAAGTATGATCTTGATTTTGAATTGCATGTTAATAATTATTAATAAACATTTGATGTGTAAATACATACTATTTCAAATGACTGATGTTGGATTCTTACCATTTTTTTTATATCTTTTGATCATAGTTAACTGGGATGAACCTGAAATCTCGAAGATTCCAAACTGTGTCAATCCTTGGCAGGTTGAACTTATTCACCACAAAGCTGAACTGTATATACAATCCCCCCCAAATAAAAATCCTAGACGTGCTGAGAGCTCTGTCCTGTTAAGTGATACCGGGGAGCTACATTCCGAAGCATTCCCCACAAAATCTATATTTACCATCCCTACCAAAATCTGGCAGAAATGTGCTGGAGCCTCAGTCAAGATTCCGAAGCTACATTCTAAAGTTTACTACTTCCCACAAGGTCACTTAGAACATGTCTCCGCTCACACAATCATCACCCTTCTTCATCGTTATCCTCCTTCCATCCTCACTCCTGTAACGGATGGCCATGGCCATGAACAAGAAGCTCCTCCAGAAGTTCTTGCCGAAGACGAGGATGGCTACAATGTTGTTTCCTTTGTCAAGATTCTCACACAATCTGATTGCAATAGTGGCTATGGATTCATTGTATCTCTCCCTTGCGTTGACTTGATCTTGCCGAAGCTTAACTTGGACGATTCAATGCCATCTCAGACACTCTCCGTCACTGACATTCAAGGCCGAGTCTGGCAGTACACGCATATCTACCGCGGTAAATCCAAGAGACACCTTTTTAGTAGGGGATGGACTTCATTTGTCAACAACAAGAAACTCGTCGCTGGTGATTCAGTTGTTTTCGTTAAAAACTCAGCTGGGAAGATATTCGTTGGAATATGCCGGAAAAGTGGGAAGGTAACAGAGAAGGCGGTTATGGATGCGGTGGAATTGGCGGTGAAAAACATGGCATTTGAAGTTTTGTATTATCCGATGCAAATGTTGTTGATGATGCCATGA
Protein sequence

>MS.gene89498.t1

MVPCALRIHQIMFNWDEPEISKIPNCVNPWQVELIHHKAELYIQSPPNKNPRRAESSVLLSDTGELHSEAFPTKSIFTIPTKIWQKCAGASVKIPKLHSKVYYFPQGHLEHVSAHTIITLLHRYPPSILTPVTDGHGHEQEAPPEVLAEDEDGYNVVSFVKILTQSDCNSGYGFIVSLPCVDLILPKLNLDDSMPSQTLSVTDIQGRVWQYTHIYRGKSKRHLFSRGWTSFVNNKKLVAGDSVVFVKNSAGKIFVGICRKSGKVTEKAVMDAVELAVKNMAFEVLYYPMQMLLMMP