AlfalfaGEDB | MS.gene89680

M. sativa cultivar XinJiangDaYe / MS.gene89680

KEGG: K08829
GO: GO:0000166 GO:0003674 GO:0003824 GO:0004672 GO:0005488 GO:0005524 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008144 GO:0008150 GO:0008152 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017076 GO:0019538 GO:0030554 GO:0032553 GO:0032555 GO:0032559 GO:0035639 GO:0036094 GO:0036211 GO:0043167 GO:0043168 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0071704 GO:0097159 GO:0097367 GO:0140096 GO:1901265 GO:1901363 GO:1901564
PFAM: PF00069.28

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene89680.t1	XP_003612616.1	94.3	88	4	1	1	87	1	88	8.40E-37	162.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene89680.t1	Q6Z8C8	78.4	88	18	1	1	87	1	88	2.8e-33	142.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene89680.t1	G7K6V4	94.3	88	4	1	1	87	1	88	6.0e-37	162.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene89680.t1	MTR_5g026960	94.318	88	4	1	1	87	1	88	2.88e-53	172
MS.gene89680.t1	MTR_8g071230	85.227	88	12	1	1	87	1	88	1.79e-48	160
MS.gene89680.t1	MTR_8g012450	54.545	88	39	1	1	87	1	88	6.37e-29	107
MS.gene89680.t1	MTR_8g012450	54.545	88	39	1	1	87	1	88	8.39e-29	107
MS.gene89680.t1	MTR_3g054080	41.860	86	47	1	2	84	12	97	1.69e-14	68.2
MS.gene89680.t1	MTR_8g461040	41.304	92	48	2	3	88	25	116	4.43e-13	64.3
MS.gene89680.t1	MTR_6g013030	38.095	84	49	2	2	82	108	191	1.61e-12	62.8
MS.gene89680.t1	MTR_1g098300	36.782	87	50	3	2	84	124	209	4.31e-12	61.2
MS.gene89680.t1	MTR_4g094430	34.940	83	53	1	1	82	1	83	1.00e-11	60.1
MS.gene89680.t1	MTR_5g013550	42.500	80	44	2	2	81	4	81	1.22e-11	60.1
MS.gene89680.t1	MTR_2g072840	39.130	92	50	2	3	88	24	115	1.67e-11	59.7
MS.gene89680.t1	MTR_2g032060	34.940	83	53	1	1	82	1	83	1.69e-11	59.3
MS.gene89680.t1	MTR_2g072840	39.130	92	50	2	3	88	24	115	1.70e-11	59.7
MS.gene89680.t1	MTR_6g080470	38.372	86	48	2	2	84	12	95	5.24e-11	58.2
MS.gene89680.t1	MTR_7g114300	35.714	84	51	2	2	82	105	188	5.46e-11	58.2
MS.gene89680.t1	MTR_7g114300	35.714	84	51	2	2	82	105	188	5.48e-11	58.2
MS.gene89680.t1	MTR_7g114300	35.714	84	51	2	2	82	105	188	5.67e-11	58.2
MS.gene89680.t1	MTR_7g114300	35.714	84	51	2	2	82	105	188	5.76e-11	58.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene89680.t1	AT4G19110	86.364	88	11	1	1	87	1	88	1.05e-50	166
MS.gene89680.t1	AT4G19110	86.364	88	11	1	1	87	1	88	1.12e-50	166
MS.gene89680.t1	AT5G45430	80.682	88	16	1	1	87	1	88	1.76e-46	155
MS.gene89680.t1	AT5G45430	80.682	88	16	1	1	87	1	88	1.80e-46	155
MS.gene89680.t1	AT4G13020	64.634	82	29	0	1	82	1	82	7.28e-36	126
MS.gene89680.t1	AT4G13020	64.634	82	29	0	1	82	1	82	8.72e-36	126
MS.gene89680.t1	AT4G13020	64.634	82	29	0	1	82	1	82	1.28e-35	125
MS.gene89680.t1	AT4G13020	64.634	82	29	0	1	82	1	82	1.34e-35	126
MS.gene89680.t1	AT4G13020	63.415	82	30	0	1	82	9	90	3.77e-34	122
MS.gene89680.t1	AT4G13020	63.415	82	30	0	1	82	9	90	3.82e-34	122
MS.gene89680.t1	AT4G13020	58.242	91	29	1	1	82	1	91	1.02e-32	117
MS.gene89680.t1	AT4G13020	58.242	91	29	1	1	82	1	91	1.13e-32	118
MS.gene89680.t1	AT5G39420	40.476	84	47	2	2	82	103	186	5.87e-13	63.9
MS.gene89680.t1	AT3G01085	37.931	87	45	3	2	82	113	196	7.46e-13	63.5
MS.gene89680.t1	AT3G01085	37.931	87	45	3	2	82	113	196	7.46e-13	63.5
MS.gene89680.t1	AT3G01085	37.931	87	45	3	2	82	113	196	7.47e-13	63.5
MS.gene89680.t1	AT3G14720	39.130	92	50	2	3	88	24	115	1.80e-12	62.4
MS.gene89680.t1	AT3G14720	40.698	86	45	2	3	82	24	109	4.71e-12	61.2
MS.gene89680.t1	AT3G14720	40.698	86	45	2	3	82	24	109	4.71e-12	61.2
MS.gene89680.t1	AT1G66750	37.931	87	49	2	1	84	10	94	7.43e-12	60.5
MS.gene89680.t1	AT3G48750	36.145	83	52	1	1	82	1	83	7.56e-12	60.5
MS.gene89680.t1	AT1G53510	38.043	92	51	2	3	88	24	115	8.54e-12	60.5
MS.gene89680.t1	AT4G19110	87.500	32	3	1	57	87	1	32	9.85e-12	60.5
MS.gene89680.t1	AT1G67580	36.471	85	51	2	1	82	403	487	8.13e-11	57.8
MS.gene89680.t1	AT1G67580	36.471	85	51	2	1	82	403	487	8.13e-11	57.8
MS.gene89680.t1	AT1G74330	34.524	84	52	2	2	82	119	202	9.55e-11	57.8
MS.gene89680.t1	AT1G74330	34.524	84	52	2	2	82	119	202	9.61e-11	57.8
MS.gene89680.t1	AT1G74330	34.524	84	52	2	2	82	119	202	9.62e-11	57.8

Find 24 sgRNAs with CRISPR-Local

Find 39 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCCTTCAGCTTCACAATATT+TGG	0.040143	3.4:-45739195	None:intergenic
GTAATACCTTGACTACTCTT+AGG	0.294259	3.4:-45739058	None:intergenic
GAAGTTGGTGATGGAACATT+TGG	0.311288	3.4:+45738862	MS.gene89680:CDS
CCTTCAGCTTCACAATATTT+GGG	0.345373	3.4:-45739194	None:intergenic
TGAAGAAGAAATATTACTCC+TGG	0.433782	3.4:+45739021	MS.gene89680:CDS
ATGGAACATTTGGGAGTGTC+TGG	0.443342	3.4:+45738872	MS.gene89680:CDS
TGTCCGACACAAACATGGTT+GGG	0.452336	3.4:-45739419	None:intergenic
AAGTTGGTGATGGAACATTT+GGG	0.510987	3.4:+45738863	MS.gene89680:CDS
AATCATACTTACAACTTCAC+CGG	0.516771	3.4:-45738914	None:intergenic
TACAAAATAATCAAGGAAGT+TGG	0.517126	3.4:+45738847	MS.gene89680:CDS
TAGGTTTACGCACTCCTCCC+AGG	0.526948	3.4:-45739039	None:intergenic
TTGTCCGACACAAACATGGT+TGG	0.556533	3.4:-45739420	None:intergenic
AACATTGTCCGACACAAACA+TGG	0.557828	3.4:-45739424	None:intergenic
ACAAAAGACAGAATATCTAC+AGG	0.563192	3.4:+45739278	MS.gene89680:CDS
CTACAGGTACAAAATAATCA+AGG	0.564500	3.4:+45738840	MS.gene89680:intron
GAAGAAGAAATATTACTCCT+GGG	0.573081	3.4:+45739022	MS.gene89680:CDS
CGTAAACCTAAGAGTAGTCA+AGG	0.574821	3.4:+45739052	MS.gene89680:CDS
TCAGCTTCACAATATTTGGG+TGG	0.576856	3.4:-45739191	None:intergenic
ATAATCAAGGAAGTTGGTGA+TGG	0.578661	3.4:+45738853	MS.gene89680:CDS
AGAGCTATTAGTAAGCAAAC+CGG	0.583714	3.4:+45738895	MS.gene89680:CDS
TGTTTGTGTCAGATATGTTG+TGG	0.598784	3.4:+45739383	MS.gene89680:intron
CCCAAATATTGTGAAGCTGA+AGG	0.606272	3.4:+45739194	MS.gene89680:CDS
AATCCCAACCATGTTTGTGT+CGG	0.642986	3.4:+45739416	MS.gene89680:CDS
GAAGAAATATTACTCCTGGG+AGG	0.712706	3.4:+45739025	MS.gene89680:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AAGTTGTAAGTATGATTTAC+TGG	+	chr3.4:45738920-45738939	MS.gene89680:intron	25.0%
!	TAATCAGTTACAAAATTTGC+AGG	+	chr3.4:45738983-45739002	MS.gene89680:intron	25.0%
!	TACAAAATAATCAAGGAAGT+TGG	+	chr3.4:45738847-45738866	MS.gene89680:CDS	25.0%
!	TATACAACTCTTTATTAGGA+AGG	+	chr3.4:45739311-45739330	MS.gene89680:intron	25.0%
!	TGTGTATACAACTCTTTATT+AGG	+	chr3.4:45739307-45739326	MS.gene89680:intron	25.0%
!	TTACTTCATACTTATCTTTC+TGG	+	chr3.4:45739077-45739096	MS.gene89680:intron	25.0%
!!	TAATTTTCTCATTTACTGTG+TGG	+	chr3.4:45738792-45738811	MS.gene89680:intron	25.0%
!!	TGCAAATTTTGTAACTGATT+AGG	-	chr3.4:45738984-45739003	None:intergenic	25.0%
	AATCATACTTACAACTTCAC+CGG	-	chr3.4:45738917-45738936	None:intergenic	30.0%
	ACAAAAGACAGAATATCTAC+AGG	+	chr3.4:45739278-45739297	MS.gene89680:CDS	30.0%
	ATAACGCATAACAACAACAA+AGG	-	chr3.4:45739105-45739124	None:intergenic	30.0%
	CTACAGGTACAAAATAATCA+AGG	+	chr3.4:45738840-45738859	MS.gene89680:intron	30.0%
	GAAGAAGAAATATTACTCCT+GGG	+	chr3.4:45739022-45739041	MS.gene89680:CDS	30.0%
	TGAAGAAGAAATATTACTCC+TGG	+	chr3.4:45739021-45739040	MS.gene89680:CDS	30.0%
!	GTAGATATTCTGTCTTTTGT+TGG	-	chr3.4:45739278-45739297	None:intergenic	30.0%
!!!	GTACTTTGTTTTTGAGTACA+TGG	+	chr3.4:45739242-45739261	MS.gene89680:CDS	30.0%
	AGAGCTATTAGTAAGCAAAC+CGG	+	chr3.4:45738895-45738914	MS.gene89680:CDS	35.0%
	CCTTCAGCTTCACAATATTT+GGG	-	chr3.4:45739197-45739216	None:intergenic	35.0%
	GTAATACCTTGACTACTCTT+AGG	-	chr3.4:45739061-45739080	None:intergenic	35.0%
	TCCTTCAGCTTCACAATATT+TGG	-	chr3.4:45739198-45739217	None:intergenic	35.0%
	TGTTTGTGTCAGATATGTTG+TGG	+	chr3.4:45739383-45739402	MS.gene89680:intron	35.0%
!	ACATTTTTCCTTGACACTAC+AGG	+	chr3.4:45738824-45738843	MS.gene89680:intron	35.0%
!	ATTTTGTACCTGTAGTGTCA+AGG	-	chr3.4:45738835-45738854	None:intergenic	35.0%
!!	AAGTTGGTGATGGAACATTT+GGG	+	chr3.4:45738863-45738882	MS.gene89680:CDS	35.0%
!!	ATAATCAAGGAAGTTGGTGA+TGG	+	chr3.4:45738853-45738872	MS.gene89680:CDS	35.0%
	AACATTGTCCGACACAAACA+TGG	-	chr3.4:45739427-45739446	None:intergenic	40.0%
	AATCCCAACCATGTTTGTGT+CGG	+	chr3.4:45739416-45739435	MS.gene89680:CDS	40.0%
	CCCAAATATTGTGAAGCTGA+AGG	+	chr3.4:45739194-45739213	MS.gene89680:CDS	40.0%
	CGTAAACCTAAGAGTAGTCA+AGG	+	chr3.4:45739052-45739071	MS.gene89680:CDS	40.0%
	GAAGAAATATTACTCCTGGG+AGG	+	chr3.4:45739025-45739044	MS.gene89680:CDS	40.0%
	TCAGCTTCACAATATTTGGG+TGG	-	chr3.4:45739194-45739213	None:intergenic	40.0%
!!	GAAGTTGGTGATGGAACATT+TGG	+	chr3.4:45738862-45738881	MS.gene89680:CDS	40.0%
	TGTCCGACACAAACATGGTT+GGG	-	chr3.4:45739422-45739441	None:intergenic	45.0%
	TTCGTAAAGACTGCCAGCAA+GGG	-	chr3.4:45739164-45739183	None:intergenic	45.0%
	TTGTCCGACACAAACATGGT+TGG	-	chr3.4:45739423-45739442	None:intergenic	45.0%
	TTTCGTAAAGACTGCCAGCA+AGG	-	chr3.4:45739165-45739184	None:intergenic	45.0%
!	TGATCTCTTGTTTCCCTTGC+TGG	+	chr3.4:45739148-45739167	MS.gene89680:intron	45.0%
!!	ATGGAACATTTGGGAGTGTC+TGG	+	chr3.4:45738872-45738891	MS.gene89680:CDS	45.0%
	TAGGTTTACGCACTCCTCCC+AGG	-	chr3.4:45739042-45739061	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.4	gene	45738759	45739448	45738759	ID=MS.gene89680
chr3.4	mRNA	45738759	45739448	45738759	ID=MS.gene89680.t1;Parent=MS.gene89680
chr3.4	exon	45738759	45738766	45738759	ID=MS.gene89680.t1.exon1;Parent=MS.gene89680.t1
chr3.4	CDS	45738759	45738766	45738759	ID=cds.MS.gene89680.t1;Parent=MS.gene89680.t1
chr3.4	exon	45738846	45738924	45738846	ID=MS.gene89680.t1.exon2;Parent=MS.gene89680.t1
chr3.4	CDS	45738846	45738924	45738846	ID=cds.MS.gene89680.t1;Parent=MS.gene89680.t1
chr3.4	exon	45739005	45739073	45739005	ID=MS.gene89680.t1.exon3;Parent=MS.gene89680.t1
chr3.4	CDS	45739005	45739073	45739005	ID=cds.MS.gene89680.t1;Parent=MS.gene89680.t1
chr3.4	exon	45739174	45739299	45739174	ID=MS.gene89680.t1.exon4;Parent=MS.gene89680.t1
chr3.4	CDS	45739174	45739299	45739174	ID=cds.MS.gene89680.t1;Parent=MS.gene89680.t1
chr3.4	exon	45739395	45739448	45739395	ID=MS.gene89680.t1.exon5;Parent=MS.gene89680.t1
chr3.4	CDS	45739395	45739448	45739395	ID=cds.MS.gene89680.t1;Parent=MS.gene89680.t1

Gene Sequence

>MS.gene89680

ATGGAGAGGTGATTCATTTTCTGAAGAAATTACTAATTTTCTCATTTACTGTGTGGACTATTTTAACATTTTTCCTTGACACTACAGGTACAAAATAATCAAGGAAGTTGGTGATGGAACATTTGGGAGTGTCTGGAGAGCTATTAGTAAGCAAACCGGTGAAGTTGTAAGTATGATTTACTGGTATGTTGAAGTTTTCTCTATTTGAAGTGACATTTTTCTCCTAATCAGTTACAAAATTTGCAGGCTGCGATCAAGAAAATGAAGAAGAAATATTACTCCTGGGAGGAGTGCGTAAACCTAAGAGTAGTCAAGGTATTACTTCATACTTATCTTTCTGGAGCCTTTGTTGTTGTTATGCGTTATTATATTCTTTTGTATTTAGTCTCTGATCTCTTGTTTCCCTTGCTGGCAGTCTTTACGAAAAATGAACCACCCAAATATTGTGAAGCTGAAGGAAGTTATTCGAGAAAGTGACATTTTGTACTTTGTTTTTGAGTACATGGTGACATTGTACCAACAAAAGACAGAATATCTACAGGTGTGTATGTGTATACAACTCTTTATTAGGAAGGTGACATTGTATTTCATGATTCAAGTCGAGTCACTCATGTTATTTCATTATGTTTGTGTCAGATATGTTGTGGAATGCTGCTGAATCCCAACCATGTTTGTGTCGGACAATGTTAA

Protein sequence

>MS.gene89680.t1

MERYKIIKEVGDGTFGSVWRAISKQTGEVAAIKKMKKKYYSWEECVNLRVVKSLRKMNHPNIVKLKEVIRESDILYFVFEYMVTLYQQKTEYLQICCGMLLNPNHVCVGQC