Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89777.t1 | RHN56340.1 | 49.2 | 120 | 53 | 1 | 47 | 166 | 45 | 156 | 1.60E-21 | 113.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89777.t1 | A0A396HSM2 | 49.2 | 120 | 53 | 1 | 47 | 166 | 45 | 156 | 1.2e-21 | 113.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene89777.t1 | TF | C2H2 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89777.t1 | MTR_5g068000 | 45.522 | 134 | 64 | 2 | 38 | 170 | 35 | 160 | 1.58e-30 | 115 |
| MS.gene89777.t1 | MTR_0044s0120 | 53.571 | 56 | 26 | 0 | 3 | 58 | 2 | 57 | 1.01e-16 | 75.9 |
| MS.gene89777.t1 | MTR_0044s0120 | 50.877 | 57 | 26 | 1 | 233 | 287 | 2 | 58 | 6.96e-14 | 68.2 |
| MS.gene89777.t1 | MTR_4g088560 | 74.359 | 39 | 10 | 0 | 188 | 226 | 117 | 155 | 1.70e-11 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 59 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACAATACCTGATCTGTTTC+TGG | 0.135061 | 4.4:+83646580 | None:intergenic |
| AAATATACCTGCGATCTTAA+AGG | 0.232251 | 4.4:+83645335 | None:intergenic |
| TCTTTCCAAAACTAATATAT+TGG | 0.238647 | 4.4:-83646644 | MS.gene89777:CDS |
| AGGATCCAATATATTAGTTT+TGG | 0.244123 | 4.4:+83646639 | None:intergenic |
| GGTTGGTTTGGACCATTAAT+TGG | 0.269670 | 4.4:+83644782 | None:intergenic |
| CCCCAACAGTCACGTTTGTT+GGG | 0.299044 | 4.4:+83642806 | None:intergenic |
| AAAAGACACACAAAATCTTT+TGG | 0.334321 | 4.4:+83638078 | None:intergenic |
| TTTCACGTCCCCAAGCAATT+TGG | 0.345197 | 4.4:+83645369 | None:intergenic |
| ACTCTTCATTTGCGACTGTT+AGG | 0.354176 | 4.4:-83642899 | MS.gene89777:CDS |
| CATCTTCCAGAGCCAATTAA+TGG | 0.357102 | 4.4:-83644794 | MS.gene89777:CDS |
| CGATTTCTTGTGCTAATCAT+AGG | 0.360691 | 4.4:-83660607 | MS.gene89777:CDS |
| ATAATTTGATTGCTGAAGAT+AGG | 0.362047 | 4.4:+83644751 | None:intergenic |
| TTTCACATTCCATCATTTGA+TGG | 0.372512 | 4.4:+83638184 | None:intergenic |
| CTGCGATCTTAAAGGTTGAT+TGG | 0.389534 | 4.4:+83645343 | None:intergenic |
| TAGGGTAACACTGGTTGGTT+TGG | 0.395356 | 4.4:+83644770 | None:intergenic |
| ATCTTTGCTTTGAATGTGGC+AGG | 0.397240 | 4.4:-83638236 | MS.gene89777:CDS |
| TCCCCAACAGTCACGTTTGT+TGG | 0.397482 | 4.4:+83642805 | None:intergenic |
| ATTATAGGTCCAAATTGCTT+GGG | 0.412553 | 4.4:-83645378 | MS.gene89777:intron |
| AAAAGCATCAACTCGGCATA+TGG | 0.422839 | 4.4:+83646615 | None:intergenic |
| TATTATAGGTCCAAATTGCT+TGG | 0.426107 | 4.4:-83645379 | MS.gene89777:intron |
| GCCGCTCCAACTGCCGCAAA+AGG | 0.436342 | 4.4:+83642863 | None:intergenic |
| CATAGCGGAGATAATGATTC+CGG | 0.436862 | 4.4:+83647604 | None:intergenic |
| ATCTCCGCTATGTTCTTCAA+TGG | 0.449521 | 4.4:-83647593 | MS.gene89777:intron |
| TTGATACATTCTTCCGAAGT+AGG | 0.455125 | 4.4:+83638039 | None:intergenic |
| TTTGGACCATTAATTGGCTC+TGG | 0.464673 | 4.4:+83644788 | None:intergenic |
| CATTAATTGGCTCTGGAAGA+TGG | 0.465912 | 4.4:+83644795 | None:intergenic |
| TTCACATTCCATCATTTGAT+GGG | 0.494517 | 4.4:+83638185 | None:intergenic |
| CCCAACAGTCACGTTTGTTG+GGG | 0.500328 | 4.4:+83642807 | None:intergenic |
| AATGATTCCGGTGTGGTGAT+TGG | 0.505971 | 4.4:+83647616 | None:intergenic |
| TTTACATTCCATCATTCGAT+GGG | 0.516085 | 4.4:+83660673 | None:intergenic |
| TAATTTGATTGCTGAAGATA+GGG | 0.520089 | 4.4:+83644752 | None:intergenic |
| TTTGATTCCAGAAACAGATC+AGG | 0.522895 | 4.4:-83646587 | MS.gene89777:intron |
| TGATAGTGTATTTCCTACTT+CGG | 0.523889 | 4.4:-83638052 | MS.gene89777:CDS |
| CAATCAACCTTTAAGATCGC+AGG | 0.526484 | 4.4:-83645342 | MS.gene89777:intron |
| TGATCTGTTTCTGGAATCAA+AGG | 0.557683 | 4.4:+83646589 | None:intergenic |
| TTTGAATACCCATCAAATGA+TGG | 0.561576 | 4.4:-83638193 | MS.gene89777:CDS |
| GAAGATAGGGTAACACTGGT+TGG | 0.562971 | 4.4:+83644765 | None:intergenic |
| AAAGCAAAGATGAATTGTCA+TGG | 0.570914 | 4.4:+83638248 | None:intergenic |
| ATTCATCTTTGCTTTGAATG+TGG | 0.573954 | 4.4:-83638240 | MS.gene89777:CDS |
| ATCTTTGCTTTGAATGTGGT+AGG | 0.574496 | 4.4:-83660724 | MS.gene89777:CDS |
| ATTCATCTTTGCTTTGAATG+TGG | 0.581405 | 4.4:-83660728 | MS.gene89777:CDS |
| TGGAGCGGCTGTTCCACGAA+GGG | 0.587349 | 4.4:-83642849 | MS.gene89777:CDS |
| GCATCAACTCGGCATATGGA+AGG | 0.588578 | 4.4:+83646619 | None:intergenic |
| TTGGAGCGGCTGTTCCACGA+AGG | 0.600775 | 4.4:-83642850 | MS.gene89777:CDS |
| TTTGAATGCCCATCGAATGA+TGG | 0.609016 | 4.4:-83660681 | MS.gene89777:CDS |
| AAAGCAAAGATGAATCGTCA+TGG | 0.609504 | 4.4:+83660736 | None:intergenic |
| TTCTTGTCCGTTAAACGACA+TGG | 0.611538 | 4.4:+83643568 | None:intergenic |
| TGCTGAAGATAGGGTAACAC+TGG | 0.614288 | 4.4:+83644761 | None:intergenic |
| TTATAGGTCCAAATTGCTTG+GGG | 0.620684 | 4.4:-83645377 | MS.gene89777:intron |
| GGCAGGACCTTCAAAAGTGT+TGG | 0.632505 | 4.4:-83638219 | MS.gene89777:CDS |
| GACACACGCTGCAATAAATG+TGG | 0.640734 | 4.4:-83638159 | MS.gene89777:CDS |
| GACACACGCTGCAATAAATG+TGG | 0.662134 | 4.4:-83660647 | MS.gene89777:CDS |
| GGTAGGACCTTCAAAAGTGT+TGG | 0.663077 | 4.4:-83660707 | MS.gene89777:CDS |
| ATCAGTGAAGATTAGCCATG+TGG | 0.666114 | 4.4:+83638016 | None:intergenic |
| TTCGAGACCAATCACCACAC+CGG | 0.668576 | 4.4:-83647623 | MS.gene89777:intron |
| CGGAGATAATGATTCCGGTG+TGG | 0.696511 | 4.4:+83647609 | None:intergenic |
| AAATTATCATGCTCAAAACG+AGG | 0.697419 | 4.4:-83644735 | MS.gene89777:intron |
| GGAAGAATGTATCAACCACA+TGG | 0.708434 | 4.4:-83638031 | MS.gene89777:CDS |
| CTTACCATTGAAGAACATAG+CGG | 0.774671 | 4.4:+83647589 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 83638017 | 83660756 | 83638017 | ID=MS.gene89777 |
| chr4.4 | mRNA | 83638017 | 83660756 | 83638017 | ID=MS.gene89777.t1;Parent=MS.gene89777 |
| chr4.4 | exon | 83660571 | 83660756 | 83660571 | ID=MS.gene89777.t1.exon1;Parent=MS.gene89777.t1 |
| chr4.4 | CDS | 83660571 | 83660756 | 83660571 | ID=cds.MS.gene89777.t1;Parent=MS.gene89777.t1 |
| chr4.4 | exon | 83647594 | 83647639 | 83647594 | ID=MS.gene89777.t1.exon2;Parent=MS.gene89777.t1 |
| chr4.4 | CDS | 83647594 | 83647639 | 83647594 | ID=cds.MS.gene89777.t1;Parent=MS.gene89777.t1 |
| chr4.4 | exon | 83646588 | 83646676 | 83646588 | ID=MS.gene89777.t1.exon3;Parent=MS.gene89777.t1 |
| chr4.4 | CDS | 83646588 | 83646676 | 83646588 | ID=cds.MS.gene89777.t1;Parent=MS.gene89777.t1 |
| chr4.4 | exon | 83645343 | 83645393 | 83645343 | ID=MS.gene89777.t1.exon4;Parent=MS.gene89777.t1 |
| chr4.4 | CDS | 83645343 | 83645393 | 83645343 | ID=cds.MS.gene89777.t1;Parent=MS.gene89777.t1 |
| chr4.4 | exon | 83644736 | 83644855 | 83644736 | ID=MS.gene89777.t1.exon5;Parent=MS.gene89777.t1 |
| chr4.4 | CDS | 83644736 | 83644855 | 83644736 | ID=cds.MS.gene89777.t1;Parent=MS.gene89777.t1 |
| chr4.4 | exon | 83643565 | 83643621 | 83643565 | ID=MS.gene89777.t1.exon6;Parent=MS.gene89777.t1 |
| chr4.4 | CDS | 83643565 | 83643621 | 83643565 | ID=cds.MS.gene89777.t1;Parent=MS.gene89777.t1 |
| chr4.4 | exon | 83642819 | 83642951 | 83642819 | ID=MS.gene89777.t1.exon7;Parent=MS.gene89777.t1 |
| chr4.4 | CDS | 83642819 | 83642951 | 83642819 | ID=cds.MS.gene89777.t1;Parent=MS.gene89777.t1 |
| chr4.4 | exon | 83638017 | 83638276 | 83638017 | ID=MS.gene89777.t1.exon8;Parent=MS.gene89777.t1 |
| chr4.4 | CDS | 83638017 | 83638276 | 83638017 | ID=cds.MS.gene89777.t1;Parent=MS.gene89777.t1 |
| Gene Sequence |
| Protein sequence |
