AlfalfaGEDB | MS.gene89809

M. sativa cultivar XinJiangDaYe / MS.gene89809

KEGG: -
GO: GO:0003674 GO:0003676 GO:0005488 GO:0097159 GO:1901363
PFAM: PF13912.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene89809.t1	XP_024626703.1	85	214	30	2	1	213	1	213	2.80E-97	364.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene89809.t1	Q39266	32.6	132	80	4	9	136	5	131	4.6e-08	59.7

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene89809.t1	A0A396GYM7	85.0	214	30	2	1	213	1	213	2.0e-97	364.8

TFs/TRs:

Gene ID	Type	Classification
MS.gene89809.t1	TF	C2H2

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene89809.t1	MTR_2g067740	73.364	214	56	1	1	213	1	214	3.78e-99	286
MS.gene89809.t1	MTR_3g012010	38.596	114	55	5	58	160	98	207	3.39e-13	67.4
MS.gene89809.t1	MTR_3g009410	42.667	75	38	2	58	131	98	168	1.04e-12	66.2
MS.gene89809.t1	MTR_7g022980	33.600	125	63	5	55	174	63	172	1.04e-12	65.9
MS.gene89809.t1	MTR_5g008280	36.522	115	55	6	57	160	45	152	3.02e-12	65.1
MS.gene89809.t1	MTR_7g023010	32.800	125	64	5	55	174	63	172	9.07e-12	63.2
MS.gene89809.t1	MTR_7g022960	30.282	142	77	6	56	192	84	208	5.21e-11	61.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene89809.t1	AT1G24625	38.095	105	59	3	34	137	33	132	1.10e-12	64.7

Find 62 sgRNAs with CRISPR-Local

Find 70 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTGAAATTTGGTGGCTATTT+GGG	0.249196	7.3:+41605445	None:intergenic
CTGAATAATAATGTGGGATT+TGG	0.253595	7.3:-41605558	MS.gene89809:CDS
CCATATCCCTTCACTGTTGC+AGG	0.257859	7.3:-41605690	MS.gene89809:CDS
CATTATTATTCAGTGGAGTT+TGG	0.280641	7.3:+41605568	None:intergenic
CTCATGGATCTTGAGGGATT+TGG	0.283300	7.3:+41605890	None:intergenic
CAGCTGCAGGTTCCTTCATT+TGG	0.286157	7.3:-41605516	MS.gene89809:CDS
CAAGGAATGAGGTTGAAATT+TGG	0.293587	7.3:+41605433	None:intergenic
GTTGAAATTTGGTGGCTATT+TGG	0.298879	7.3:+41605444	None:intergenic
CCTGCAACAGTGAAGGGATA+TGG	0.307330	7.3:+41605690	None:intergenic
TATGGGTGATAAATTGAATA+TGG	0.313679	7.3:+41605708	None:intergenic
CATCCAAGTCCATGATAAAA+AGG	0.315893	7.3:+41605660	None:intergenic
GAAATTCAATTAGGAACGTT+TGG	0.316298	7.3:-41605753	MS.gene89809:CDS
ACGTCATTGTCTCTGAGTTT+AGG	0.351844	7.3:-41605939	MS.gene89809:CDS
GCAAGCTATGACAACCCATT+TGG	0.366612	7.3:-41605618	MS.gene89809:CDS
ATTATTATTCAGTGGAGTTT+GGG	0.370973	7.3:+41605569	None:intergenic
AATTAGGAACGTTTGGAGTT+GGG	0.379249	7.3:-41605746	MS.gene89809:CDS
TCATAGCTTGCATGGGCAAC+AGG	0.381549	7.3:+41605630	None:intergenic
CAAGCACTCTTTCACGCCTA+TGG	0.392813	7.3:+41605790	None:intergenic
CAATTAGGAACGTTTGGAGT+TGG	0.396900	7.3:-41605747	MS.gene89809:CDS
TTCTTGTTGCAAAACTTACA+TGG	0.408494	7.3:+41605852	None:intergenic
CAAGCTATGACAACCCATTT+GGG	0.409161	7.3:-41605617	MS.gene89809:CDS
GCTATTTGGGTCATCATGAT+TGG	0.436051	7.3:+41605458	None:intergenic
TCAGCTCCTCATGGATCTTG+AGG	0.451104	7.3:+41605883	None:intergenic
CAGCTCCTCATGGATCTTGA+GGG	0.457928	7.3:+41605884	None:intergenic
GGATGATCAATAACTCTGCT+TGG	0.462130	7.3:-41605596	MS.gene89809:CDS
ATGTCAGAAACACTACGATT+TGG	0.469253	7.3:-41606032	MS.gene89809:CDS
CTCTTAACAATCATGTGCTA+AGG	0.471735	7.3:-41605488	MS.gene89809:CDS
GAGTTATTGATCATCCCAAA+TGG	0.474296	7.3:+41605603	None:intergenic
CTGCAACAGTGAAGGGATAT+GGG	0.476975	7.3:+41605691	None:intergenic
GGTATTCCCTCAGCTCCTCA+TGG	0.496808	7.3:+41605874	None:intergenic
ATTAGGAACGTTTGGAGTTG+GGG	0.505080	7.3:-41605745	MS.gene89809:CDS
GGCACCTTGGTGTCCTCCCA+AGG	0.506085	7.3:+41605812	None:intergenic
GATGATCAATAACTCTGCTT+GGG	0.511350	7.3:-41605595	MS.gene89809:CDS
TAGGGACAAATCAAGGAATG+AGG	0.515985	7.3:+41605422	None:intergenic
TGAACTTAATAATCAGCTGC+AGG	0.516698	7.3:-41605529	MS.gene89809:CDS
CTTGCATGGGCAACAGGTTG+AGG	0.519092	7.3:+41605636	None:intergenic
AGTTATTGATCATCCCAAAT+GGG	0.522275	7.3:+41605604	None:intergenic
ATGGGTTGTCATAGCTTGCA+TGG	0.525223	7.3:+41605622	None:intergenic
AGTTTAGGAGGAGGGAGAAG+TGG	0.525434	7.3:-41605924	MS.gene89809:CDS
TCTTAACAATCATGTGCTAA+GGG	0.533636	7.3:-41605487	MS.gene89809:CDS
GGAATGAGGTTGAAATTTGG+TGG	0.534438	7.3:+41605436	None:intergenic
AACTCCACTGAATAATAATG+TGG	0.547835	7.3:-41605565	MS.gene89809:CDS
AAGCACTCTTTCACGCCTAT+GGG	0.549509	7.3:+41605791	None:intergenic
TCTTGTTGCAAAACTTACAT+GGG	0.552429	7.3:+41605853	None:intergenic
AATCCCACATTATTATTCAG+TGG	0.569517	7.3:+41605561	None:intergenic
TTAAGAGCAACACCAAATGA+AGG	0.575359	7.3:+41605504	None:intergenic
TGACGACCCTGCAACAGTGA+AGG	0.582185	7.3:+41605683	None:intergenic
CAAGATCCATGAGGAGCTGA+GGG	0.584633	7.3:-41605880	MS.gene89809:CDS
TCATTGTCTCTGAGTTTAGG+AGG	0.586488	7.3:-41605936	MS.gene89809:CDS
TTTCACGCCTATGGGCACCT+TGG	0.587966	7.3:+41605799	None:intergenic
GACGACCCTGCAACAGTGAA+GGG	0.590012	7.3:+41605684	None:intergenic
GAGGACACCAAGGTGCCCAT+AGG	0.592853	7.3:-41605806	MS.gene89809:CDS
TCAAGATCCATGAGGAGCTG+AGG	0.596229	7.3:-41605881	MS.gene89809:CDS
TGTCTCTGAGTTTAGGAGGA+GGG	0.598792	7.3:-41605932	MS.gene89809:CDS
TCAAGTTCTCAAGCCTTGGG+AGG	0.602598	7.3:-41605825	MS.gene89809:CDS
TTGTCTCTGAGTTTAGGAGG+AGG	0.607557	7.3:-41605933	MS.gene89809:CDS
TGGGTTGTCATAGCTTGCAT+GGG	0.610755	7.3:+41605623	None:intergenic
CATATCCCTTCACTGTTGCA+GGG	0.613658	7.3:-41605689	MS.gene89809:CDS
ACTCCACTGAATAATAATGT+GGG	0.629245	7.3:-41605564	MS.gene89809:CDS
CTTAACAATCATGTGCTAAG+GGG	0.643060	7.3:-41605486	MS.gene89809:CDS
CAAGCCTTGGGAGGACACCA+AGG	0.644910	7.3:-41605816	MS.gene89809:CDS
CAAATCCCTCAAGATCCATG+AGG	0.742811	7.3:-41605889	MS.gene89809:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATTGAAAAAGAAATTCAATT+AGG	-	chr7.3:41605683-41605702	MS.gene89809:CDS	15.0%
!	ATTATTATTCAGTGGAGTTT+GGG	+	chr7.3:41605879-41605898	None:intergenic	25.0%
!	TATGGGTGATAAATTGAATA+TGG	+	chr7.3:41605740-41605759	None:intergenic	25.0%
	AACTCCACTGAATAATAATG+TGG	-	chr7.3:41605880-41605899	MS.gene89809:CDS	30.0%
	AATCCCACATTATTATTCAG+TGG	+	chr7.3:41605887-41605906	None:intergenic	30.0%
	ACTCCACTGAATAATAATGT+GGG	-	chr7.3:41605881-41605900	MS.gene89809:CDS	30.0%
	AGTTATTGATCATCCCAAAT+GGG	+	chr7.3:41605844-41605863	None:intergenic	30.0%
	CATTATTATTCAGTGGAGTT+TGG	+	chr7.3:41605880-41605899	None:intergenic	30.0%
	CTGAATAATAATGTGGGATT+TGG	-	chr7.3:41605887-41605906	MS.gene89809:CDS	30.0%
	GAAATTCAATTAGGAACGTT+TGG	-	chr7.3:41605692-41605711	MS.gene89809:CDS	30.0%
	TCTTAACAATCATGTGCTAA+GGG	-	chr7.3:41605958-41605977	MS.gene89809:CDS	30.0%
	TCTTGTTGCAAAACTTACAT+GGG	+	chr7.3:41605595-41605614	None:intergenic	30.0%
	TTCTTGTTGCAAAACTTACA+TGG	+	chr7.3:41605596-41605615	None:intergenic	30.0%
!	TTGAAATTTGGTGGCTATTT+GGG	+	chr7.3:41606003-41606022	None:intergenic	30.0%
!!!	AAAGTTTTAGGGACAAATCA+AGG	+	chr7.3:41606033-41606052	None:intergenic	30.0%
	ATGTCAGAAACACTACGATT+TGG	-	chr7.3:41605413-41605432	MS.gene89809:CDS	35.0%
	CAAGGAATGAGGTTGAAATT+TGG	+	chr7.3:41606015-41606034	None:intergenic	35.0%
	CATCCAAGTCCATGATAAAA+AGG	+	chr7.3:41605788-41605807	None:intergenic	35.0%
	CTCTTAACAATCATGTGCTA+AGG	-	chr7.3:41605957-41605976	MS.gene89809:CDS	35.0%
	CTTAACAATCATGTGCTAAG+GGG	-	chr7.3:41605959-41605978	MS.gene89809:CDS	35.0%
	GAGTTATTGATCATCCCAAA+TGG	+	chr7.3:41605845-41605864	None:intergenic	35.0%
	GATGATCAATAACTCTGCTT+GGG	-	chr7.3:41605850-41605869	MS.gene89809:CDS	35.0%
	TGAACTTAATAATCAGCTGC+AGG	-	chr7.3:41605916-41605935	MS.gene89809:CDS	35.0%
	TTAAGAGCAACACCAAATGA+AGG	+	chr7.3:41605944-41605963	None:intergenic	35.0%
!	AATTAGGAACGTTTGGAGTT+GGG	-	chr7.3:41605699-41605718	MS.gene89809:CDS	35.0%
!	CTTTTTATCATGGACTTGGA+TGG	-	chr7.3:41605786-41605805	MS.gene89809:CDS	35.0%
!	GTTGAAATTTGGTGGCTATT+TGG	+	chr7.3:41606004-41606023	None:intergenic	35.0%
!	TCACCTTTTTATCATGGACT+TGG	-	chr7.3:41605782-41605801	MS.gene89809:CDS	35.0%
!!!	TTTTCAAGTTCTCAAGCCTT+GGG	-	chr7.3:41605617-41605636	MS.gene89809:CDS	35.0%
	ACGTCATTGTCTCTGAGTTT+AGG	-	chr7.3:41605506-41605525	MS.gene89809:CDS	40.0%
	CAAAAAGCTAAAGCGCAAGA+TGG	-	chr7.3:41605457-41605476	MS.gene89809:CDS	40.0%
	CAAGCTATGACAACCCATTT+GGG	-	chr7.3:41605828-41605847	MS.gene89809:CDS	40.0%
	GCTATTTGGGTCATCATGAT+TGG	+	chr7.3:41605990-41606009	None:intergenic	40.0%
	GGAATGAGGTTGAAATTTGG+TGG	+	chr7.3:41606012-41606031	None:intergenic	40.0%
	GGATGATCAATAACTCTGCT+TGG	-	chr7.3:41605849-41605868	MS.gene89809:CDS	40.0%
	TAGGGACAAATCAAGGAATG+AGG	+	chr7.3:41606026-41606045	None:intergenic	40.0%
	TCATTGTCTCTGAGTTTAGG+AGG	-	chr7.3:41605509-41605528	MS.gene89809:CDS	40.0%
!	ATTAGGAACGTTTGGAGTTG+GGG	-	chr7.3:41605700-41605719	MS.gene89809:CDS	40.0%
!	CAATTAGGAACGTTTGGAGT+TGG	-	chr7.3:41605698-41605717	MS.gene89809:CDS	40.0%
!!!	GTTTTCAAGTTCTCAAGCCT+TGG	-	chr7.3:41605616-41605635	MS.gene89809:CDS	40.0%
	AAGCACTCTTTCACGCCTAT+GGG	+	chr7.3:41605657-41605676	None:intergenic	45.0%
	CAAATCCCTCAAGATCCATG+AGG	-	chr7.3:41605556-41605575	MS.gene89809:CDS	45.0%
	CATATCCCTTCACTGTTGCA+GGG	-	chr7.3:41605756-41605775	MS.gene89809:CDS	45.0%
	CTCATGGATCTTGAGGGATT+TGG	+	chr7.3:41605558-41605577	None:intergenic	45.0%
	CTGCAACAGTGAAGGGATAT+GGG	+	chr7.3:41605757-41605776	None:intergenic	45.0%
	GCAAGCTATGACAACCCATT+TGG	-	chr7.3:41605827-41605846	MS.gene89809:CDS	45.0%
	TGTCTCTGAGTTTAGGAGGA+GGG	-	chr7.3:41605513-41605532	MS.gene89809:CDS	45.0%
	TTGTCTCTGAGTTTAGGAGG+AGG	-	chr7.3:41605512-41605531	MS.gene89809:CDS	45.0%
!	ATGGGTTGTCATAGCTTGCA+TGG	+	chr7.3:41605826-41605845	None:intergenic	45.0%
!	GGGTCGTCACCTTTTTATCA+TGG	-	chr7.3:41605776-41605795	MS.gene89809:CDS	45.0%
!	TGGGTTGTCATAGCTTGCAT+GGG	+	chr7.3:41605825-41605844	None:intergenic	45.0%
	AGTTTAGGAGGAGGGAGAAG+TGG	-	chr7.3:41605521-41605540	MS.gene89809:CDS	50.0%
	CAAGATCCATGAGGAGCTGA+GGG	-	chr7.3:41605565-41605584	MS.gene89809:CDS	50.0%
	CAAGCACTCTTTCACGCCTA+TGG	+	chr7.3:41605658-41605677	None:intergenic	50.0%
	CAGCTCCTCATGGATCTTGA+GGG	+	chr7.3:41605564-41605583	None:intergenic	50.0%
	CAGCTGCAGGTTCCTTCATT+TGG	-	chr7.3:41605929-41605948	MS.gene89809:CDS	50.0%
	CCATATCCCTTCACTGTTGC+AGG	-	chr7.3:41605755-41605774	MS.gene89809:CDS	50.0%
	CCTGCAACAGTGAAGGGATA+TGG	+	chr7.3:41605758-41605777	None:intergenic	50.0%
	TCAAGATCCATGAGGAGCTG+AGG	-	chr7.3:41605564-41605583	MS.gene89809:CDS	50.0%
	TCAAGTTCTCAAGCCTTGGG+AGG	-	chr7.3:41605620-41605639	MS.gene89809:CDS	50.0%
	TCAGCTCCTCATGGATCTTG+AGG	+	chr7.3:41605565-41605584	None:intergenic	50.0%
!	TCATAGCTTGCATGGGCAAC+AGG	+	chr7.3:41605818-41605837	None:intergenic	50.0%
	CTTGCATGGGCAACAGGTTG+AGG	+	chr7.3:41605812-41605831	None:intergenic	55.0%
	GACGACCCTGCAACAGTGAA+GGG	+	chr7.3:41605764-41605783	None:intergenic	55.0%
	GGTATTCCCTCAGCTCCTCA+TGG	+	chr7.3:41605574-41605593	None:intergenic	55.0%
	TGACGACCCTGCAACAGTGA+AGG	+	chr7.3:41605765-41605784	None:intergenic	55.0%
	TTTCACGCCTATGGGCACCT+TGG	+	chr7.3:41605649-41605668	None:intergenic	55.0%
	CAAGCCTTGGGAGGACACCA+AGG	-	chr7.3:41605629-41605648	MS.gene89809:CDS	60.0%
!	GAGGACACCAAGGTGCCCAT+AGG	-	chr7.3:41605639-41605658	MS.gene89809:CDS	60.0%
!!	GGCACCTTGGTGTCCTCCCA+AGG	+	chr7.3:41605636-41605655	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.3	gene	41605413	41606054	41605413	ID=MS.gene89809
chr7.3	mRNA	41605413	41606054	41605413	ID=MS.gene89809.t1;Parent=MS.gene89809
chr7.3	exon	41605413	41606054	41605413	ID=MS.gene89809.t1.exon1;Parent=MS.gene89809.t1
chr7.3	CDS	41605413	41606054	41605413	ID=cds.MS.gene89809.t1;Parent=MS.gene89809.t1

Gene Sequence

>MS.gene89809

ATGTCAGAAACACTACGATTTGGTGATGATGTTACAAGAGATAACAAAAAGCTAAAGCGCAAGATGGATGATGCTATTGCTGCTGATTCAGAGACGTCATTGTCTCTGAGTTTAGGAGGAGGGAGAAGTGGAGCATCTTCATCCAAATCCCTCAAGATCCATGAGGAGCTGAGGGAATACCCATGTAAGTTTTGCAACAAGAAGTTTTCAAGTTCTCAAGCCTTGGGAGGACACCAAGGTGCCCATAGGCGTGAAAGAGTGCTTGCGAGAATTGAAAAAGAAATTCAATTAGGAACGTTTGGAGTTGGGGATCATCATTTGTGTCCATATTCAATTTATCACCCATATCCCTTCACTGTTGCAGGGTCGTCACCTTTTTATCATGGACTTGGATGGCCTCAACCTGTTGCCCATGCAAGCTATGACAACCCATTTGGGATGATCAATAACTCTGCTTGGGCTACCCAAACTCCACTGAATAATAATGTGGGATTTGGAAATTATGAACTTAATAATCAGCTGCAGGTTCCTTCATTTGGTGTTGCTCTTAACAATCATGTGCTAAGGGGAAATACCAATCATGATGACCCAAATAGCCACCAAATTTCAACCTCATTCCTTGATTTGTCCCTAAAACTTTAG

Protein sequence

>MS.gene89809.t1

MSETLRFGDDVTRDNKKLKRKMDDAIAADSETSLSLSLGGGRSGASSSKSLKIHEELREYPCKFCNKKFSSSQALGGHQGAHRRERVLARIEKEIQLGTFGVGDHHLCPYSIYHPYPFTVAGSSPFYHGLGWPQPVAHASYDNPFGMINNSAWATQTPLNNNVGFGNYELNNQLQVPSFGVALNNHVLRGNTNHDDPNSHQISTSFLDLSLKL