Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89890.t1 | AES79453.1 | 93.8 | 129 | 8 | 0 | 1 | 129 | 875 | 1003 | 3.00E-62 | 247.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89890.t1 | Q9SD62 | 43.2 | 132 | 48 | 3 | 1 | 128 | 911 | 1019 | 3.2e-20 | 99.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89890.t1 | G7KRB1 | 93.8 | 129 | 8 | 0 | 1 | 129 | 875 | 1003 | 2.2e-62 | 247.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89890.t1 | MTR_7g067530 | 93.798 | 129 | 8 | 0 | 1 | 129 | 875 | 1003 | 3.61e-80 | 254 |
| MS.gene89890.t1 | MTR_6g036780 | 41.406 | 128 | 66 | 1 | 1 | 128 | 866 | 984 | 2.33e-25 | 100 |
| MS.gene89890.t1 | MTR_8g071950 | 37.692 | 130 | 72 | 3 | 1 | 130 | 30 | 150 | 3.99e-25 | 93.6 |
| MS.gene89890.t1 | MTR_6g036890 | 41.406 | 128 | 65 | 2 | 1 | 128 | 869 | 986 | 6.16e-25 | 99.0 |
| MS.gene89890.t1 | MTR_2g040910 | 41.406 | 128 | 65 | 2 | 1 | 128 | 904 | 1021 | 6.67e-25 | 99.0 |
| MS.gene89890.t1 | MTR_6g036840 | 40.625 | 128 | 66 | 2 | 1 | 128 | 904 | 1021 | 1.17e-24 | 98.2 |
| MS.gene89890.t1 | MTR_8g068540 | 41.600 | 125 | 64 | 2 | 1 | 125 | 899 | 1014 | 5.80e-24 | 96.3 |
| MS.gene89890.t1 | MTR_5g026160 | 38.281 | 128 | 66 | 2 | 1 | 128 | 875 | 989 | 6.26e-24 | 96.3 |
| MS.gene89890.t1 | MTR_5g019070 | 39.535 | 129 | 66 | 3 | 1 | 128 | 892 | 1009 | 1.06e-23 | 95.5 |
| MS.gene89890.t1 | MTR_5g026010 | 40.310 | 129 | 65 | 3 | 1 | 128 | 891 | 1008 | 2.30e-23 | 94.4 |
| MS.gene89890.t1 | MTR_5g025860 | 38.760 | 129 | 67 | 2 | 1 | 128 | 872 | 989 | 4.88e-23 | 93.6 |
| MS.gene89890.t1 | MTR_2g072600 | 40.157 | 127 | 61 | 2 | 2 | 128 | 168 | 279 | 1.25e-22 | 90.1 |
| MS.gene89890.t1 | MTR_5g026000 | 39.535 | 129 | 66 | 2 | 1 | 128 | 891 | 1008 | 1.85e-22 | 92.0 |
| MS.gene89890.t1 | MTR_5g025950 | 37.692 | 130 | 71 | 2 | 1 | 130 | 904 | 1023 | 2.29e-22 | 91.7 |
| MS.gene89890.t1 | MTR_5g026150 | 38.281 | 128 | 69 | 2 | 1 | 128 | 873 | 990 | 7.95e-22 | 90.1 |
| MS.gene89890.t1 | MTR_5g025880 | 37.984 | 129 | 68 | 2 | 1 | 128 | 1180 | 1297 | 1.20e-21 | 89.7 |
| MS.gene89890.t1 | MTR_6g036790 | 37.500 | 128 | 70 | 1 | 1 | 128 | 879 | 996 | 1.46e-21 | 89.4 |
| MS.gene89890.t1 | MTR_5g026090 | 35.156 | 128 | 69 | 1 | 1 | 128 | 837 | 950 | 1.86e-21 | 89.0 |
| MS.gene89890.t1 | MTR_8g089210 | 34.646 | 127 | 68 | 1 | 2 | 128 | 878 | 989 | 3.38e-21 | 88.2 |
| MS.gene89890.t1 | MTR_5g025930 | 35.156 | 128 | 73 | 1 | 1 | 128 | 875 | 992 | 4.71e-21 | 87.8 |
| MS.gene89890.t1 | MTR_5g025890 | 37.500 | 128 | 64 | 2 | 1 | 128 | 900 | 1011 | 5.39e-21 | 87.8 |
| MS.gene89890.t1 | MTR_5g026200 | 36.800 | 125 | 69 | 2 | 1 | 125 | 747 | 861 | 8.98e-21 | 87.0 |
| MS.gene89890.t1 | MTR_5g044680 | 33.077 | 130 | 77 | 1 | 1 | 130 | 807 | 926 | 2.16e-20 | 85.9 |
| MS.gene89890.t1 | MTR_5g044680 | 33.077 | 130 | 77 | 1 | 1 | 130 | 903 | 1022 | 2.33e-20 | 85.9 |
| MS.gene89890.t1 | MTR_5g024450 | 36.719 | 128 | 71 | 1 | 1 | 128 | 909 | 1026 | 4.14e-20 | 85.1 |
| MS.gene89890.t1 | MTR_3g070220 | 35.385 | 130 | 71 | 2 | 1 | 130 | 893 | 1009 | 5.32e-20 | 84.7 |
| MS.gene89890.t1 | MTR_1g088935 | 33.594 | 128 | 76 | 2 | 1 | 128 | 720 | 838 | 7.78e-20 | 84.3 |
| MS.gene89890.t1 | MTR_5g024420 | 34.746 | 118 | 67 | 1 | 11 | 128 | 1 | 108 | 6.47e-19 | 77.0 |
| MS.gene89890.t1 | MTR_5g025840 | 35.938 | 128 | 71 | 2 | 1 | 128 | 860 | 976 | 8.25e-19 | 81.6 |
| MS.gene89890.t1 | MTR_8g089200 | 36.885 | 122 | 62 | 2 | 2 | 123 | 903 | 1009 | 9.02e-19 | 81.3 |
| MS.gene89890.t1 | MTR_5g082275 | 36.667 | 120 | 61 | 2 | 9 | 128 | 44 | 148 | 2.13e-18 | 76.3 |
| MS.gene89890.t1 | MTR_5g025850 | 32.812 | 128 | 76 | 1 | 1 | 128 | 885 | 1002 | 2.77e-18 | 80.1 |
| MS.gene89890.t1 | MTR_5g082460 | 36.000 | 125 | 64 | 1 | 1 | 125 | 889 | 997 | 4.09e-18 | 79.3 |
| MS.gene89890.t1 | MTR_8g066700 | 34.375 | 128 | 66 | 1 | 1 | 128 | 898 | 1007 | 4.13e-18 | 79.3 |
| MS.gene89890.t1 | MTR_5g082290 | 37.097 | 124 | 63 | 2 | 2 | 125 | 896 | 1004 | 8.62e-18 | 78.6 |
| MS.gene89890.t1 | MTR_5g082320 | 36.290 | 124 | 62 | 3 | 6 | 128 | 169 | 276 | 1.04e-17 | 77.0 |
| MS.gene89890.t1 | MTR_5g082420 | 34.109 | 129 | 70 | 2 | 2 | 130 | 767 | 880 | 2.84e-17 | 77.0 |
| MS.gene89890.t1 | MTR_1g088940 | 34.375 | 128 | 75 | 2 | 1 | 128 | 938 | 1056 | 3.08e-17 | 77.0 |
| MS.gene89890.t1 | MTR_1g088940 | 34.375 | 128 | 75 | 2 | 1 | 128 | 895 | 1013 | 3.42e-17 | 77.0 |
| MS.gene89890.t1 | MTR_7g078340 | 35.833 | 120 | 62 | 2 | 9 | 128 | 832 | 936 | 1.53e-16 | 75.1 |
| MS.gene89890.t1 | MTR_3g092420 | 29.008 | 131 | 87 | 1 | 1 | 125 | 863 | 993 | 4.49e-16 | 73.6 |
| MS.gene89890.t1 | MTR_5g082920 | 34.677 | 124 | 66 | 1 | 2 | 125 | 897 | 1005 | 1.82e-15 | 72.0 |
| MS.gene89890.t1 | MTR_5g082380 | 34.959 | 123 | 65 | 2 | 6 | 128 | 151 | 258 | 2.30e-15 | 70.5 |
| MS.gene89890.t1 | MTR_1g029950 | 36.283 | 113 | 57 | 2 | 9 | 121 | 483 | 580 | 3.32e-15 | 71.2 |
| MS.gene89890.t1 | MTR_6g036870 | 46.377 | 69 | 36 | 1 | 1 | 69 | 844 | 911 | 4.27e-15 | 70.9 |
| MS.gene89890.t1 | MTR_1g029940 | 35.897 | 117 | 60 | 2 | 9 | 125 | 831 | 932 | 4.66e-15 | 70.9 |
| MS.gene89890.t1 | MTR_3g092390 | 29.323 | 133 | 88 | 1 | 1 | 127 | 908 | 1040 | 5.44e-15 | 70.5 |
| MS.gene89890.t1 | MTR_2g072620 | 37.795 | 127 | 55 | 3 | 2 | 128 | 590 | 692 | 7.15e-15 | 70.1 |
| MS.gene89890.t1 | MTR_2g072640 | 37.795 | 127 | 55 | 3 | 2 | 128 | 621 | 723 | 7.33e-15 | 70.1 |
| MS.gene89890.t1 | MTR_6g040210 | 32.540 | 126 | 58 | 2 | 3 | 128 | 747 | 845 | 1.64e-14 | 69.3 |
| MS.gene89890.t1 | MTR_3g449540 | 34.146 | 123 | 61 | 2 | 3 | 125 | 809 | 911 | 1.93e-14 | 68.9 |
| MS.gene89890.t1 | MTR_5g082270 | 36.752 | 117 | 54 | 2 | 9 | 125 | 906 | 1002 | 2.15e-14 | 68.9 |
| MS.gene89890.t1 | MTR_3g007730 | 33.884 | 121 | 65 | 2 | 6 | 126 | 376 | 481 | 5.82e-14 | 67.4 |
| MS.gene89890.t1 | MTR_0830s0010 | 32.000 | 125 | 65 | 2 | 3 | 127 | 806 | 910 | 2.38e-13 | 65.9 |
| MS.gene89890.t1 | MTR_4g029710 | 30.894 | 123 | 64 | 2 | 3 | 125 | 926 | 1027 | 3.43e-13 | 65.5 |
| MS.gene89890.t1 | MTR_8g470560 | 31.707 | 123 | 64 | 2 | 3 | 125 | 679 | 781 | 5.39e-13 | 64.7 |
| MS.gene89890.t1 | MTR_0070s0020 | 31.707 | 123 | 64 | 2 | 3 | 125 | 487 | 589 | 5.69e-13 | 64.7 |
| MS.gene89890.t1 | MTR_1g088930 | 45.763 | 59 | 32 | 0 | 1 | 59 | 898 | 956 | 8.04e-13 | 64.3 |
| MS.gene89890.t1 | MTR_8g468620 | 32.520 | 123 | 63 | 2 | 3 | 125 | 621 | 723 | 8.85e-13 | 64.3 |
| MS.gene89890.t1 | MTR_8g465340 | 31.746 | 126 | 66 | 2 | 3 | 128 | 971 | 1076 | 9.90e-13 | 63.9 |
| MS.gene89890.t1 | MTR_8g469600 | 30.894 | 123 | 65 | 2 | 3 | 125 | 749 | 851 | 1.09e-12 | 63.9 |
| MS.gene89890.t1 | MTR_8g470170 | 29.268 | 123 | 67 | 2 | 3 | 125 | 136 | 238 | 1.53e-12 | 62.4 |
| MS.gene89890.t1 | MTR_2g016580 | 33.333 | 126 | 57 | 2 | 3 | 128 | 653 | 751 | 1.59e-12 | 63.5 |
| MS.gene89890.t1 | MTR_8g470370 | 30.894 | 123 | 65 | 2 | 3 | 125 | 288 | 390 | 2.74e-12 | 62.8 |
| MS.gene89890.t1 | MTR_8g469860 | 31.496 | 127 | 61 | 4 | 3 | 126 | 74 | 177 | 2.81e-12 | 60.8 |
| MS.gene89890.t1 | MTR_1g088945 | 46.939 | 49 | 26 | 0 | 10 | 58 | 122 | 170 | 3.56e-12 | 60.5 |
| MS.gene89890.t1 | MTR_8g469830 | 32.520 | 123 | 63 | 2 | 3 | 125 | 710 | 812 | 6.90e-12 | 61.6 |
| MS.gene89890.t1 | MTR_8g470050 | 30.894 | 123 | 65 | 3 | 3 | 125 | 671 | 773 | 7.50e-12 | 61.6 |
| MS.gene89890.t1 | MTR_5g026760 | 52.083 | 48 | 23 | 0 | 1 | 48 | 584 | 631 | 1.84e-11 | 60.5 |
| MS.gene89890.t1 | MTR_8g470950 | 30.081 | 123 | 66 | 2 | 3 | 125 | 666 | 768 | 2.35e-11 | 60.1 |
| MS.gene89890.t1 | MTR_8g470400 | 31.200 | 125 | 60 | 4 | 3 | 124 | 733 | 834 | 3.76e-11 | 59.7 |
| MS.gene89890.t1 | MTR_8g469780 | 30.081 | 123 | 66 | 2 | 3 | 125 | 695 | 797 | 4.05e-11 | 59.3 |
| MS.gene89890.t1 | MTR_8g469980 | 28.455 | 123 | 68 | 2 | 3 | 125 | 563 | 665 | 7.74e-11 | 58.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89890.t1 | AT3G47110 | 43.182 | 132 | 48 | 3 | 1 | 128 | 911 | 1019 | 6.28e-25 | 99.0 |
| MS.gene89890.t1 | AT3G47570 | 41.406 | 128 | 55 | 3 | 1 | 128 | 894 | 1001 | 5.59e-23 | 93.6 |
| MS.gene89890.t1 | AT3G47580 | 39.844 | 128 | 57 | 3 | 1 | 128 | 895 | 1002 | 1.03e-21 | 89.7 |
| MS.gene89890.t1 | AT3G47090 | 37.500 | 128 | 60 | 2 | 1 | 128 | 893 | 1000 | 1.62e-20 | 86.3 |
| MS.gene89890.t1 | AT5G39390 | 31.538 | 130 | 58 | 1 | 1 | 130 | 401 | 499 | 1.65e-13 | 66.2 |
| MS.gene89890.t1 | AT5G20480 | 32.812 | 128 | 55 | 2 | 1 | 128 | 904 | 1000 | 6.07e-13 | 64.7 |
| MS.gene89890.t1 | AT5G20480 | 32.812 | 128 | 55 | 2 | 1 | 128 | 904 | 1000 | 6.07e-13 | 64.7 |
Find 27 sgRNAs with CRISPR-Local
Find 27 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTGGAGATGTTTACAGTTA+TGG | 0.261476 | 7.3:-37494088 | MS.gene89890:CDS |
| CTCTATTATCAAGAATTTGA+TGG | 0.282147 | 7.3:-37493935 | MS.gene89890:CDS |
| TTGGAGATGTTTACAGTTAT+GGG | 0.301410 | 7.3:-37494087 | MS.gene89890:CDS |
| CCTTCAAACATCTCATTTGT+TGG | 0.313693 | 7.3:+37494031 | None:intergenic |
| TTGGTTTCTGTGTTGCAAAT+TGG | 0.318775 | 7.3:-37493833 | MS.gene89890:CDS |
| TGAAGGATCAATTATATCTA+TGG | 0.337982 | 7.3:+37493961 | None:intergenic |
| ATTATATCTATGGCATGATT+TGG | 0.342777 | 7.3:+37493971 | None:intergenic |
| CTCTACTATGGAGAATTGTT+TGG | 0.347625 | 7.3:-37493852 | MS.gene89890:CDS |
| GTCATAGGTATTCGCTCATT+TGG | 0.380048 | 7.3:+37493788 | None:intergenic |
| TTGTTGACAACCACAGTCAT+AGG | 0.399884 | 7.3:+37493773 | None:intergenic |
| ACCTGGAGACTTCTCTACTA+TGG | 0.427299 | 7.3:-37493864 | MS.gene89890:CDS |
| AATTCAACAGTTTACAGCCT+TGG | 0.449124 | 7.3:-37493999 | MS.gene89890:CDS |
| CCAACAAATGAGATGTTTGA+AGG | 0.480330 | 7.3:-37494031 | MS.gene89890:CDS |
| TCTTGATAATAGAGCAATGA+AGG | 0.495687 | 7.3:+37493944 | None:intergenic |
| TTAAGGAGAGAGAAGGAACC+TGG | 0.504973 | 7.3:-37493881 | MS.gene89890:CDS |
| TAAACATCTCCAAGTGCAGA+TGG | 0.507920 | 7.3:+37494097 | None:intergenic |
| ACAGTGAAGAGATAACATTA+AGG | 0.513299 | 7.3:-37493898 | MS.gene89890:CDS |
| TCCATAGTAGAGAAGTCTCC+AGG | 0.514941 | 7.3:+37493863 | None:intergenic |
| GAGATGTTTGAAGGTGGCAT+GGG | 0.565236 | 7.3:-37494022 | MS.gene89890:CDS |
| GGTGGACACCCATCTGCACT+TGG | 0.565878 | 7.3:-37494106 | MS.gene89890:CDS |
| GATAACATTAAGGAGAGAGA+AGG | 0.585284 | 7.3:-37493888 | MS.gene89890:CDS |
| TGAGATGTTTGAAGGTGGCA+TGG | 0.592305 | 7.3:-37494023 | MS.gene89890:CDS |
| GGCATGATTTGGTAAAGCCA+AGG | 0.621657 | 7.3:+37493982 | None:intergenic |
| AAACATCTCCAAGTGCAGAT+GGG | 0.629549 | 7.3:+37494098 | None:intergenic |
| TGCAGAGTATGGAATATGGG+TGG | 0.644121 | 7.3:-37494124 | None:intergenic |
| ACAAATGAGATGTTTGAAGG+TGG | 0.668908 | 7.3:-37494028 | MS.gene89890:CDS |
| TGAGCGAATACCTATGACTG+TGG | 0.710426 | 7.3:-37493783 | MS.gene89890:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATTATATCTATGGCATGATT+TGG | + | chr7.3:37493880-37493899 | None:intergenic | 25.0% |
| ! | CTCTATTATCAAGAATTTGA+TGG | - | chr7.3:37493913-37493932 | MS.gene89890:CDS | 25.0% |
| ! | TGAAGGATCAATTATATCTA+TGG | + | chr7.3:37493890-37493909 | None:intergenic | 25.0% |
| ACAGTGAAGAGATAACATTA+AGG | - | chr7.3:37493950-37493969 | MS.gene89890:CDS | 30.0% | |
| TTGGAGATGTTTACAGTTAT+GGG | - | chr7.3:37493761-37493780 | MS.gene89890:CDS | 30.0% | |
| ! | TCTTGATAATAGAGCAATGA+AGG | + | chr7.3:37493907-37493926 | None:intergenic | 30.0% |
| AATTCAACAGTTTACAGCCT+TGG | - | chr7.3:37493849-37493868 | MS.gene89890:CDS | 35.0% | |
| ACAAATGAGATGTTTGAAGG+TGG | - | chr7.3:37493820-37493839 | MS.gene89890:CDS | 35.0% | |
| CCAACAAATGAGATGTTTGA+AGG | - | chr7.3:37493817-37493836 | MS.gene89890:CDS | 35.0% | |
| CCTTCAAACATCTCATTTGT+TGG | + | chr7.3:37493820-37493839 | None:intergenic | 35.0% | |
| CTCTACTATGGAGAATTGTT+TGG | - | chr7.3:37493996-37494015 | MS.gene89890:CDS | 35.0% | |
| CTTGGAGATGTTTACAGTTA+TGG | - | chr7.3:37493760-37493779 | MS.gene89890:CDS | 35.0% | |
| GATAACATTAAGGAGAGAGA+AGG | - | chr7.3:37493960-37493979 | MS.gene89890:CDS | 35.0% | |
| TTGGTTTCTGTGTTGCAAAT+TGG | - | chr7.3:37494015-37494034 | MS.gene89890:CDS | 35.0% | |
| ! | CTATTGCTAGAGATTTTCAC+AGG | - | chr7.3:37493787-37493806 | MS.gene89890:CDS | 35.0% |
| AAACATCTCCAAGTGCAGAT+GGG | + | chr7.3:37493753-37493772 | None:intergenic | 40.0% | |
| GTCATAGGTATTCGCTCATT+TGG | + | chr7.3:37494063-37494082 | None:intergenic | 40.0% | |
| TAAACATCTCCAAGTGCAGA+TGG | + | chr7.3:37493754-37493773 | None:intergenic | 40.0% | |
| TTGTTGACAACCACAGTCAT+AGG | + | chr7.3:37494078-37494097 | None:intergenic | 40.0% | |
| ACCTGGAGACTTCTCTACTA+TGG | - | chr7.3:37493984-37494003 | MS.gene89890:CDS | 45.0% | |
| GAGATGTTTGAAGGTGGCAT+GGG | - | chr7.3:37493826-37493845 | MS.gene89890:CDS | 45.0% | |
| TCCATAGTAGAGAAGTCTCC+AGG | + | chr7.3:37493988-37494007 | None:intergenic | 45.0% | |
| TGAGATGTTTGAAGGTGGCA+TGG | - | chr7.3:37493825-37493844 | MS.gene89890:CDS | 45.0% | |
| TGAGCGAATACCTATGACTG+TGG | - | chr7.3:37494065-37494084 | MS.gene89890:CDS | 45.0% | |
| TTAAGGAGAGAGAAGGAACC+TGG | - | chr7.3:37493967-37493986 | MS.gene89890:CDS | 45.0% | |
| !! | GGCATGATTTGGTAAAGCCA+AGG | + | chr7.3:37493869-37493888 | None:intergenic | 45.0% |
| GGTGGACACCCATCTGCACT+TGG | - | chr7.3:37493742-37493761 | MS.gene89890:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 37493739 | 37494131 | 37493739 | ID=MS.gene89890 |
| chr7.3 | mRNA | 37493739 | 37494131 | 37493739 | ID=MS.gene89890.t1;Parent=MS.gene89890 |
| chr7.3 | exon | 37493739 | 37494131 | 37493739 | ID=MS.gene89890.t1.exon1;Parent=MS.gene89890.t1 |
| chr7.3 | CDS | 37493739 | 37494131 | 37493739 | ID=cds.MS.gene89890.t1;Parent=MS.gene89890.t1 |
| Gene Sequence |
| Protein sequence |
