Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90005.t1 | RHN79225.1 | 78.9 | 303 | 45 | 4 | 1 | 301 | 1 | 286 | 5.90E-85 | 324.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90005.t1 | A0A396JSM8 | 78.9 | 303 | 45 | 4 | 1 | 301 | 1 | 286 | 4.2e-85 | 324.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene90005.t1 | TF | C2H2 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90005.t1 | MTR_1g053530 | 53.252 | 246 | 85 | 8 | 18 | 258 | 16 | 236 | 8.79e-68 | 212 |
| MS.gene90005.t1 | MTR_1g046810 | 32.588 | 313 | 137 | 11 | 27 | 299 | 37 | 315 | 7.01e-33 | 123 |
| MS.gene90005.t1 | MTR_1g052185 | 33.451 | 284 | 140 | 15 | 28 | 297 | 30 | 278 | 1.64e-23 | 97.4 |
| MS.gene90005.t1 | MTR_4g073310 | 28.028 | 289 | 130 | 8 | 11 | 298 | 16 | 227 | 3.43e-12 | 65.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 58 sgRNAs with CRISPR-Local
Find 63 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATAATTCCAGCTCCGTTTCT+TGG | 0.269877 | 1.4:-40275706 | None:intergenic |
| GAAGAAAGTTGTTGCCTCTA+TGG | 0.295075 | 1.4:-40275458 | None:intergenic |
| AGAGGCAACAACTTTCTTCT+AGG | 0.323367 | 1.4:+40275462 | MS.gene90005:CDS |
| ACTCTCATCACAAACCAAAA+AGG | 0.357704 | 1.4:+40275343 | MS.gene90005:CDS |
| TCTTCGAAGTCAAACCATAT+TGG | 0.359710 | 1.4:-40275605 | None:intergenic |
| TGAGGAACCAACTCATGAAT+TGG | 0.362086 | 1.4:+40276112 | MS.gene90005:CDS |
| GTTCTCTTCATTATCATCAT+TGG | 0.362989 | 1.4:-40276021 | None:intergenic |
| TGCAGAAATGTGGGTGGGAT+TGG | 0.366750 | 1.4:+40275834 | MS.gene90005:CDS |
| ATGAAATCGCACAGCCAATA+TGG | 0.368027 | 1.4:+40275591 | MS.gene90005:CDS |
| TCAACTTCACCAGTGTTGTT+TGG | 0.375897 | 1.4:-40276080 | None:intergenic |
| ATCATTGGAAGCAGATTGAT+TGG | 0.381296 | 1.4:-40276006 | None:intergenic |
| GAGGAACCAACTCATGAATT+GGG | 0.385643 | 1.4:+40276113 | MS.gene90005:CDS |
| TGATTCCCATCAAGGTTGTT+AGG | 0.398895 | 1.4:-40275786 | None:intergenic |
| AAATACTTTCTCAAATCAAT+TGG | 0.400225 | 1.4:-40275663 | None:intergenic |
| CGATCACCTAACAACCTTGA+TGG | 0.406250 | 1.4:+40275780 | MS.gene90005:CDS |
| GCAGAAATGTGGGTGGGATT+GGG | 0.432815 | 1.4:+40275835 | MS.gene90005:CDS |
| TGCCTCTATGGAATGCTTTG+TGG | 0.438332 | 1.4:-40275446 | None:intergenic |
| TCATCTGTCAATGCACTGAA+TGG | 0.463276 | 1.4:+40275564 | MS.gene90005:CDS |
| TTCTTGGTCACATAACTCAT+TGG | 0.463818 | 1.4:-40275690 | None:intergenic |
| TCATGTTGCAAAAGCAATGC+TGG | 0.472244 | 1.4:+40275746 | MS.gene90005:CDS |
| ATATGGTTTGACTTCGAAGA+AGG | 0.481456 | 1.4:+40275608 | MS.gene90005:CDS |
| TACTTTCTCAAATCAATTGG+TGG | 0.486384 | 1.4:-40275660 | None:intergenic |
| GAATGCTTTGTGGCCGCCGA+GGG | 0.493083 | 1.4:-40275436 | None:intergenic |
| TTTAACCCCAATTCATGAGT+TGG | 0.505846 | 1.4:-40276119 | None:intergenic |
| CAATTGGTGGAAATTGATCC+AGG | 0.509751 | 1.4:-40275647 | None:intergenic |
| GGAATCACCGTGTCACGAAA+AGG | 0.512728 | 1.4:+40275802 | MS.gene90005:CDS |
| GATCAAACTTTGCAGAAATG+TGG | 0.524856 | 1.4:+40275824 | MS.gene90005:CDS |
| TGACACGGTGATTCCCATCA+AGG | 0.527477 | 1.4:-40275794 | None:intergenic |
| TTGGTCACATAACTCATTGG+TGG | 0.531076 | 1.4:-40275687 | None:intergenic |
| GATCACCTAACAACCTTGAT+GGG | 0.547419 | 1.4:+40275781 | MS.gene90005:CDS |
| TTCACCACTGGTAACGCCCT+CGG | 0.552306 | 1.4:+40275420 | MS.gene90005:CDS |
| ATCAAACTTTGCAGAAATGT+GGG | 0.554714 | 1.4:+40275825 | MS.gene90005:CDS |
| TGAGTTATGTGACCAAGAAA+CGG | 0.560971 | 1.4:+40275694 | MS.gene90005:CDS |
| AACTTTGCAGAAATGTGGGT+GGG | 0.561670 | 1.4:+40275829 | MS.gene90005:CDS |
| AAAAGGAGTGTTGTTGATCA+TGG | 0.562216 | 1.4:+40275360 | MS.gene90005:CDS |
| GGGTAGTAAGATTTATGACC+TGG | 0.563188 | 1.4:+40275629 | MS.gene90005:CDS |
| AAACAATGAAAAGAACAAGT+TGG | 0.564169 | 1.4:+40275932 | MS.gene90005:CDS |
| TGAAGTTGATGAAGCATATG+AGG | 0.573810 | 1.4:+40276094 | MS.gene90005:CDS |
| TCATCTGCATCATCATCCAC+TGG | 0.575486 | 1.4:-40276173 | None:intergenic |
| TATGGTTTGACTTCGAAGAA+GGG | 0.580570 | 1.4:+40275609 | MS.gene90005:CDS |
| GGAATGCTTTGTGGCCGCCG+AGG | 0.588007 | 1.4:-40275437 | None:intergenic |
| GCAACGAACACATGAATGGT+TGG | 0.590907 | 1.4:-40275529 | None:intergenic |
| AAAAGAACAAGTTGGTGGTG+AGG | 0.597114 | 1.4:+40275940 | MS.gene90005:CDS |
| CGATTTGAATGAGCTGCCAG+TGG | 0.597243 | 1.4:+40276157 | MS.gene90005:CDS |
| ATGTGACCAAGAAACGGAGC+TGG | 0.597485 | 1.4:+40275700 | MS.gene90005:CDS |
| AAGATGAAGATCAGAGAGCA+AGG | 0.598147 | 1.4:+40275858 | MS.gene90005:CDS |
| GCCGCCGAGGGCGTTACCAG+TGG | 0.610498 | 1.4:-40275424 | None:intergenic |
| TGTGACAGAGAATTCACCAC+TGG | 0.614086 | 1.4:+40275408 | MS.gene90005:CDS |
| CAATGAAAAGAACAAGTTGG+TGG | 0.617009 | 1.4:+40275935 | MS.gene90005:CDS |
| AAACTTTGCAGAAATGTGGG+TGG | 0.635743 | 1.4:+40275828 | MS.gene90005:CDS |
| AGGAACCAACTCATGAATTG+GGG | 0.638568 | 1.4:+40276114 | MS.gene90005:CDS |
| GGCCACAAAGCATTCCATAG+AGG | 0.645608 | 1.4:+40275444 | MS.gene90005:CDS |
| ACTGGTGAAGATGATGAAGC+AGG | 0.649250 | 1.4:+40276050 | MS.gene90005:CDS |
| TGCTGCAACGAACACATGAA+TGG | 0.655011 | 1.4:-40275533 | None:intergenic |
| AATGAAGAGAACAACAACAC+TGG | 0.663304 | 1.4:+40276032 | MS.gene90005:CDS |
| GGTGAGGAACCAAACAACAC+TGG | 0.673005 | 1.4:+40276071 | MS.gene90005:CDS |
| ACCACTGGTAACGCCCTCGG+CGG | 0.680554 | 1.4:+40275423 | MS.gene90005:CDS |
| TGAAGATGATGAAGCAGGTG+AGG | 0.685127 | 1.4:+40276055 | MS.gene90005:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATACTTTCTCAAATCAAT+TGG | - | chr1.4:40275666-40275685 | None:intergenic | 20.0% |
| ! | AAACAATGAAAAGAACAAGT+TGG | + | chr1.4:40275932-40275951 | MS.gene90005:CDS | 25.0% |
| AACAAAGAAAAACATGCTGA+TGG | + | chr1.4:40275492-40275511 | MS.gene90005:CDS | 30.0% | |
| ATCAAACTTTGCAGAAATGT+GGG | + | chr1.4:40275825-40275844 | MS.gene90005:CDS | 30.0% | |
| GTTCTCTTCATTATCATCAT+TGG | - | chr1.4:40276024-40276043 | None:intergenic | 30.0% | |
| TACTTTCTCAAATCAATTGG+TGG | - | chr1.4:40275663-40275682 | None:intergenic | 30.0% | |
| ! | CTTGTGATTTTTGAATGACA+AGG | - | chr1.4:40275983-40276002 | None:intergenic | 30.0% |
| ! | TTTTGAATGACAAGGCTATT+CGG | - | chr1.4:40275975-40275994 | None:intergenic | 30.0% |
| AATGAAGAGAACAACAACAC+TGG | + | chr1.4:40276032-40276051 | MS.gene90005:CDS | 35.0% | |
| ACTCTCATCACAAACCAAAA+AGG | + | chr1.4:40275343-40275362 | MS.gene90005:CDS | 35.0% | |
| ATCATTGGAAGCAGATTGAT+TGG | - | chr1.4:40276009-40276028 | None:intergenic | 35.0% | |
| GATCAAACTTTGCAGAAATG+TGG | + | chr1.4:40275824-40275843 | MS.gene90005:CDS | 35.0% | |
| TCTTCGAAGTCAAACCATAT+TGG | - | chr1.4:40275608-40275627 | None:intergenic | 35.0% | |
| TGAGTTATGTGACCAAGAAA+CGG | + | chr1.4:40275694-40275713 | MS.gene90005:CDS | 35.0% | |
| TTCTTGGTCACATAACTCAT+TGG | - | chr1.4:40275693-40275712 | None:intergenic | 35.0% | |
| TTTAACCCCAATTCATGAGT+TGG | - | chr1.4:40276122-40276141 | None:intergenic | 35.0% | |
| ! | ATATGGTTTGACTTCGAAGA+AGG | + | chr1.4:40275608-40275627 | MS.gene90005:CDS | 35.0% |
| ! | CAATGAAAAGAACAAGTTGG+TGG | + | chr1.4:40275935-40275954 | MS.gene90005:CDS | 35.0% |
| ! | TATGGTTTGACTTCGAAGAA+GGG | + | chr1.4:40275609-40275628 | MS.gene90005:CDS | 35.0% |
| ! | TGATCAACAACACTCCTTTT+TGG | - | chr1.4:40275360-40275379 | None:intergenic | 35.0% |
| !! | AAAAGGAGTGTTGTTGATCA+TGG | + | chr1.4:40275360-40275379 | MS.gene90005:CDS | 35.0% |
| !! | TGAAGTTGATGAAGCATATG+AGG | + | chr1.4:40276094-40276113 | MS.gene90005:CDS | 35.0% |
| AAACTTTGCAGAAATGTGGG+TGG | + | chr1.4:40275828-40275847 | MS.gene90005:CDS | 40.0% | |
| AACTTTGCAGAAATGTGGGT+GGG | + | chr1.4:40275829-40275848 | MS.gene90005:CDS | 40.0% | |
| AAGATGAAGATCAGAGAGCA+AGG | + | chr1.4:40275858-40275877 | MS.gene90005:CDS | 40.0% | |
| AGAGGCAACAACTTTCTTCT+AGG | + | chr1.4:40275462-40275481 | MS.gene90005:CDS | 40.0% | |
| AGGAACCAACTCATGAATTG+GGG | + | chr1.4:40276114-40276133 | MS.gene90005:CDS | 40.0% | |
| ATAATTCCAGCTCCGTTTCT+TGG | - | chr1.4:40275709-40275728 | None:intergenic | 40.0% | |
| ATGAAATCGCACAGCCAATA+TGG | + | chr1.4:40275591-40275610 | MS.gene90005:CDS | 40.0% | |
| CAATTGGTGGAAATTGATCC+AGG | - | chr1.4:40275650-40275669 | None:intergenic | 40.0% | |
| GAAGAAAGTTGTTGCCTCTA+TGG | - | chr1.4:40275461-40275480 | None:intergenic | 40.0% | |
| GAGGAACCAACTCATGAATT+GGG | + | chr1.4:40276113-40276132 | MS.gene90005:CDS | 40.0% | |
| GATCACCTAACAACCTTGAT+GGG | + | chr1.4:40275781-40275800 | MS.gene90005:CDS | 40.0% | |
| GGGTAGTAAGATTTATGACC+TGG | + | chr1.4:40275629-40275648 | MS.gene90005:CDS | 40.0% | |
| TCATCTGTCAATGCACTGAA+TGG | + | chr1.4:40275564-40275583 | MS.gene90005:CDS | 40.0% | |
| TCATGTTGCAAAAGCAATGC+TGG | + | chr1.4:40275746-40275765 | MS.gene90005:CDS | 40.0% | |
| TGAGGAACCAACTCATGAAT+TGG | + | chr1.4:40276112-40276131 | MS.gene90005:CDS | 40.0% | |
| TGATTCCCATCAAGGTTGTT+AGG | - | chr1.4:40275789-40275808 | None:intergenic | 40.0% | |
| TTGGTCACATAACTCATTGG+TGG | - | chr1.4:40275690-40275709 | None:intergenic | 40.0% | |
| ! | GTTTGATCCTTTTCGTGACA+CGG | - | chr1.4:40275812-40275831 | None:intergenic | 40.0% |
| ! | TCAACTTCACCAGTGTTGTT+TGG | - | chr1.4:40276083-40276102 | None:intergenic | 40.0% |
| !! | AAAAGAACAAGTTGGTGGTG+AGG | + | chr1.4:40275940-40275959 | MS.gene90005:CDS | 40.0% |
| ACTGGTGAAGATGATGAAGC+AGG | + | chr1.4:40276050-40276069 | MS.gene90005:CDS | 45.0% | |
| CGATCACCTAACAACCTTGA+TGG | + | chr1.4:40275780-40275799 | MS.gene90005:CDS | 45.0% | |
| GCAACGAACACATGAATGGT+TGG | - | chr1.4:40275532-40275551 | None:intergenic | 45.0% | |
| TCATCTGCATCATCATCCAC+TGG | - | chr1.4:40276176-40276195 | None:intergenic | 45.0% | |
| TGAAGATGATGAAGCAGGTG+AGG | + | chr1.4:40276055-40276074 | MS.gene90005:CDS | 45.0% | |
| TGCCTCTATGGAATGCTTTG+TGG | - | chr1.4:40275449-40275468 | None:intergenic | 45.0% | |
| TGCTGCAACGAACACATGAA+TGG | - | chr1.4:40275536-40275555 | None:intergenic | 45.0% | |
| TGTGACAGAGAATTCACCAC+TGG | + | chr1.4:40275408-40275427 | MS.gene90005:CDS | 45.0% | |
| ATGTGACCAAGAAACGGAGC+TGG | + | chr1.4:40275700-40275719 | MS.gene90005:CDS | 50.0% | |
| CGATTTGAATGAGCTGCCAG+TGG | + | chr1.4:40276157-40276176 | MS.gene90005:CDS | 50.0% | |
| GCAGAAATGTGGGTGGGATT+GGG | + | chr1.4:40275835-40275854 | MS.gene90005:CDS | 50.0% | |
| GGAATCACCGTGTCACGAAA+AGG | + | chr1.4:40275802-40275821 | MS.gene90005:CDS | 50.0% | |
| GGTGAGGAACCAAACAACAC+TGG | + | chr1.4:40276071-40276090 | MS.gene90005:CDS | 50.0% | |
| TGACACGGTGATTCCCATCA+AGG | - | chr1.4:40275797-40275816 | None:intergenic | 50.0% | |
| TGCAGAAATGTGGGTGGGAT+TGG | + | chr1.4:40275834-40275853 | MS.gene90005:CDS | 50.0% | |
| ! | GGCCACAAAGCATTCCATAG+AGG | + | chr1.4:40275444-40275463 | MS.gene90005:CDS | 50.0% |
| ! | TTCACCACTGGTAACGCCCT+CGG | + | chr1.4:40275420-40275439 | MS.gene90005:CDS | 55.0% |
| GAATGCTTTGTGGCCGCCGA+GGG | - | chr1.4:40275439-40275458 | None:intergenic | 60.0% | |
| GGAATGCTTTGTGGCCGCCG+AGG | - | chr1.4:40275440-40275459 | None:intergenic | 65.0% | |
| ! | ACCACTGGTAACGCCCTCGG+CGG | + | chr1.4:40275423-40275442 | MS.gene90005:CDS | 65.0% |
| !! | GCCGCCGAGGGCGTTACCAG+TGG | - | chr1.4:40275427-40275446 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 40275303 | 40276208 | 40275303 | ID=MS.gene90005 |
| chr1.4 | mRNA | 40275303 | 40276208 | 40275303 | ID=MS.gene90005.t1;Parent=MS.gene90005 |
| chr1.4 | exon | 40275303 | 40276208 | 40275303 | ID=MS.gene90005.t1.exon1;Parent=MS.gene90005.t1 |
| chr1.4 | CDS | 40275303 | 40276208 | 40275303 | ID=cds.MS.gene90005.t1;Parent=MS.gene90005.t1 |
| Gene Sequence |
| Protein sequence |
