Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90006.t1 | KEH41670.1 | 60.4 | 270 | 74 | 6 | 1 | 261 | 1 | 246 | 1.10E-66 | 263.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90006.t1 | A0A072VU80 | 60.4 | 270 | 74 | 6 | 1 | 261 | 1 | 246 | 8.3e-67 | 263.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene90006.t1 | TF | C2H2 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90006.t1 | MTR_1g053530 | 72.112 | 251 | 41 | 5 | 20 | 261 | 16 | 246 | 2.30e-117 | 337 |
| MS.gene90006.t1 | MTR_1g046810 | 35.235 | 298 | 133 | 9 | 29 | 278 | 30 | 315 | 8.15e-42 | 146 |
| MS.gene90006.t1 | MTR_1g052185 | 31.884 | 276 | 134 | 12 | 29 | 273 | 23 | 275 | 2.64e-25 | 102 |
| MS.gene90006.t1 | MTR_4g073310 | 31.746 | 252 | 124 | 9 | 28 | 277 | 22 | 227 | 3.03e-20 | 87.0 |
| MS.gene90006.t1 | MTR_1g085590 | 31.472 | 197 | 96 | 9 | 88 | 273 | 73 | 241 | 2.89e-11 | 63.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 51 sgRNAs with CRISPR-Local
Find 57 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCTTGGTCTCGTATCTTAT+TGG | 0.208428 | 1.4:-40282189 | None:intergenic |
| AGCACCAACAACTTTCTTAA+AGG | 0.236987 | 1.4:+40281964 | MS.gene90006:CDS |
| TACCCTTCTTCGAACCATTT+TGG | 0.254107 | 1.4:-40282110 | None:intergenic |
| ATAATCCCAGCTCCGTTTCT+TGG | 0.287583 | 1.4:-40282205 | None:intergenic |
| ATGAAATGGCACAGCCAAAA+TGG | 0.332105 | 1.4:+40282096 | MS.gene90006:CDS |
| GTTCTCTACATTGTCATCAT+TGG | 0.335954 | 1.4:-40282475 | None:intergenic |
| GTTTGAGTAATAAGTCCTTT+TGG | 0.343516 | 1.4:-40282426 | None:intergenic |
| TGAATCACCGTGACTGTAAA+AGG | 0.346155 | 1.4:+40282304 | MS.gene90006:CDS |
| CAAATTTAAACGCTAAATAG+TGG | 0.394892 | 1.4:-40282610 | None:intergenic |
| TTTACCTTTAAGAAAGTTGT+TGG | 0.419418 | 1.4:-40281968 | None:intergenic |
| TCATCTACAACATCATTTGC+CGG | 0.440063 | 1.4:-40282585 | None:intergenic |
| TAAGAAAGTTGTTGGTGCTA+TGG | 0.440297 | 1.4:-40281960 | None:intergenic |
| CTTTGAATGGACACATGAAA+TGG | 0.459517 | 1.4:+40282082 | MS.gene90006:CDS |
| CTCTACATTGTCATCATTGG+AGG | 0.472478 | 1.4:-40282472 | None:intergenic |
| TTGGTCTCGTATCTTATTGG+TGG | 0.473127 | 1.4:-40282186 | None:intergenic |
| ACGGTGATTCACATTAAGAT+TGG | 0.473501 | 1.4:-40282292 | None:intergenic |
| CAATGGATGGAAACTGATCT+AGG | 0.474117 | 1.4:-40282146 | None:intergenic |
| ATCCGTTTCATCACCTTCAT+CGG | 0.483860 | 1.4:-40282502 | None:intergenic |
| TGGTGCTATGGAATTGTTTG+TGG | 0.484496 | 1.4:-40281948 | None:intergenic |
| CAGCCAAAATGGTTCGAAGA+AGG | 0.488255 | 1.4:+40282107 | MS.gene90006:CDS |
| TGTGCTAGAGAATTCACAAC+TGG | 0.492819 | 1.4:+40281910 | MS.gene90006:CDS |
| CAAATACTTTGTCAGATCAA+TGG | 0.494617 | 1.4:-40282163 | None:intergenic |
| GAATTGTTTGTGGCCGCCGA+GGG | 0.497780 | 1.4:-40281938 | None:intergenic |
| TACCGATGAAGGTGATGAAA+CGG | 0.519602 | 1.4:+40282500 | MS.gene90006:CDS |
| GTGAGATTAAAGATTCCAAA+AGG | 0.530313 | 1.4:+40282411 | MS.gene90006:CDS |
| ACGAGACCAAGAAACGGAGC+TGG | 0.539882 | 1.4:+40282199 | MS.gene90006:CDS |
| AAAGACAAACATGCTGATGA+TGG | 0.542758 | 1.4:+40281997 | MS.gene90006:CDS |
| ACATTGTCATCATTGGAGGC+AGG | 0.544139 | 1.4:-40282468 | None:intergenic |
| TGTTAACATTGCTGAAACAA+TGG | 0.555726 | 1.4:+40282245 | MS.gene90006:CDS |
| GGAATTGTTTGTGGCCGCCG+AGG | 0.556033 | 1.4:-40281939 | None:intergenic |
| TTCACAACTGGTAATGCCCT+CGG | 0.558819 | 1.4:+40281922 | MS.gene90006:CDS |
| AGCCAAAATGGTTCGAAGAA+GGG | 0.561339 | 1.4:+40282108 | MS.gene90006:CDS |
| ATGAAGGTGATGAAACGGAT+GGG | 0.561647 | 1.4:+40282505 | MS.gene90006:CDS |
| TGAATAGAATGATGAGAAGA+AGG | 0.561791 | 1.4:-40281808 | None:intergenic |
| GCTGATGATGGTATTAAGAT+CGG | 0.564146 | 1.4:+40282009 | MS.gene90006:CDS |
| GTAGAGAACAATACCGATGA+AGG | 0.568109 | 1.4:+40282489 | MS.gene90006:CDS |
| TTCATGTGTTCTTTGCAACA+AGG | 0.570989 | 1.4:+40282041 | MS.gene90006:CDS |
| CGAGACCAAGAAACGGAGCT+GGG | 0.594941 | 1.4:+40282200 | MS.gene90006:CDS |
| CAATGAAAAGAACAGGTTGG+TGG | 0.599420 | 1.4:+40282389 | MS.gene90006:CDS |
| TACTTTGTCAGATCAATGGA+TGG | 0.603001 | 1.4:-40282159 | None:intergenic |
| TGAAGGTGATGAAACGGATG+GGG | 0.603593 | 1.4:+40282506 | MS.gene90006:CDS |
| TAAGATACGAGACCAAGAAA+CGG | 0.610128 | 1.4:+40282193 | MS.gene90006:CDS |
| GCAAAGAACACATGAATGGT+TGG | 0.613792 | 1.4:-40282034 | None:intergenic |
| GATGAAGGTGATGAAACGGA+TGG | 0.616100 | 1.4:+40282504 | MS.gene90006:CDS |
| AAACAATGAAAAGAACAGGT+TGG | 0.620052 | 1.4:+40282386 | MS.gene90006:CDS |
| TGTTGCAAAGAACACATGAA+TGG | 0.632355 | 1.4:-40282038 | None:intergenic |
| TAACATTGCTGAAACAATGG+TGG | 0.675593 | 1.4:+40282248 | MS.gene90006:CDS |
| ACAACTGGTAATGCCCTCGG+CGG | 0.704742 | 1.4:+40281925 | MS.gene90006:CDS |
| GAAAATGAGAAACATCAACA+AGG | 0.726705 | 1.4:+40281862 | MS.gene90006:CDS |
| GATGTGAAGATCAATGAACA+AGG | 0.760498 | 1.4:+40282357 | MS.gene90006:CDS |
| GTGAAGATCAATGAACAAGG+TGG | 0.773369 | 1.4:+40282360 | MS.gene90006:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | GTAAAAACAATGAAAAGAAC+AGG | + | chr1.4:40282382-40282401 | MS.gene90006:CDS | 25.0% |
| !! | ATTTTCATGATCAACAACAT+TGG | - | chr1.4:40281848-40281867 | None:intergenic | 25.0% |
| !! | TTTACCTTTAAGAAAGTTGT+TGG | - | chr1.4:40281971-40281990 | None:intergenic | 25.0% |
| AAACAATGAAAAGAACAGGT+TGG | + | chr1.4:40282386-40282405 | MS.gene90006:CDS | 30.0% | |
| CAAATACTTTGTCAGATCAA+TGG | - | chr1.4:40282166-40282185 | None:intergenic | 30.0% | |
| GAAAATGAGAAACATCAACA+AGG | + | chr1.4:40281862-40281881 | MS.gene90006:CDS | 30.0% | |
| GTGAGATTAAAGATTCCAAA+AGG | + | chr1.4:40282411-40282430 | MS.gene90006:CDS | 30.0% | |
| TGAATAGAATGATGAGAAGA+AGG | - | chr1.4:40281811-40281830 | None:intergenic | 30.0% | |
| TGTTAACATTGCTGAAACAA+TGG | + | chr1.4:40282245-40282264 | MS.gene90006:CDS | 30.0% | |
| ! | ATTCGAGTCAAACATTTTTC+AGG | + | chr1.4:40282542-40282561 | MS.gene90006:CDS | 30.0% |
| ! | GTTTGAGTAATAAGTCCTTT+TGG | - | chr1.4:40282429-40282448 | None:intergenic | 30.0% |
| !! | TCATCGAAAAAAGCTTTGAA+TGG | + | chr1.4:40282069-40282088 | MS.gene90006:CDS | 30.0% |
| !!! | TGATCAACAACATTGGTTTT+TGG | - | chr1.4:40281841-40281860 | None:intergenic | 30.0% |
| !!! | TTTTGATTTGAATGAGCTAC+CGG | + | chr1.4:40282566-40282585 | MS.gene90006:CDS | 30.0% |
| AAAGACAAACATGCTGATGA+TGG | + | chr1.4:40281997-40282016 | MS.gene90006:CDS | 35.0% | |
| ACGGTGATTCACATTAAGAT+TGG | - | chr1.4:40282295-40282314 | None:intergenic | 35.0% | |
| AGCACCAACAACTTTCTTAA+AGG | + | chr1.4:40281964-40281983 | MS.gene90006:CDS | 35.0% | |
| CTTTGAATGGACACATGAAA+TGG | + | chr1.4:40282082-40282101 | MS.gene90006:CDS | 35.0% | |
| GATGTGAAGATCAATGAACA+AGG | + | chr1.4:40282357-40282376 | MS.gene90006:CDS | 35.0% | |
| GTTCTCTACATTGTCATCAT+TGG | - | chr1.4:40282478-40282497 | None:intergenic | 35.0% | |
| TAACATTGCTGAAACAATGG+TGG | + | chr1.4:40282248-40282267 | MS.gene90006:CDS | 35.0% | |
| TAAGATACGAGACCAAGAAA+CGG | + | chr1.4:40282193-40282212 | MS.gene90006:CDS | 35.0% | |
| TACTTTGTCAGATCAATGGA+TGG | - | chr1.4:40282162-40282181 | None:intergenic | 35.0% | |
| TCATCTACAACATCATTTGC+CGG | - | chr1.4:40282588-40282607 | None:intergenic | 35.0% | |
| TGTTGCAAAGAACACATGAA+TGG | - | chr1.4:40282041-40282060 | None:intergenic | 35.0% | |
| TTCATGTGTTCTTTGCAACA+AGG | + | chr1.4:40282041-40282060 | MS.gene90006:CDS | 35.0% | |
| TTCTTGGTCTCGTATCTTAT+TGG | - | chr1.4:40282192-40282211 | None:intergenic | 35.0% | |
| ! | GTTTCATCCTTTTACAGTCA+CGG | - | chr1.4:40282314-40282333 | None:intergenic | 35.0% |
| !! | GCTGATGATGGTATTAAGAT+CGG | + | chr1.4:40282009-40282028 | MS.gene90006:CDS | 35.0% |
| !! | TAAGAAAGTTGTTGGTGCTA+TGG | - | chr1.4:40281963-40281982 | None:intergenic | 35.0% |
| AGCCAAAATGGTTCGAAGAA+GGG | + | chr1.4:40282108-40282127 | MS.gene90006:CDS | 40.0% | |
| ATCCGTTTCATCACCTTCAT+CGG | - | chr1.4:40282505-40282524 | None:intergenic | 40.0% | |
| ATGAAATGGCACAGCCAAAA+TGG | + | chr1.4:40282096-40282115 | MS.gene90006:CDS | 40.0% | |
| ATGAAGGTGATGAAACGGAT+GGG | + | chr1.4:40282505-40282524 | MS.gene90006:CDS | 40.0% | |
| CAATGGATGGAAACTGATCT+AGG | - | chr1.4:40282149-40282168 | None:intergenic | 40.0% | |
| CTCTACATTGTCATCATTGG+AGG | - | chr1.4:40282475-40282494 | None:intergenic | 40.0% | |
| GCAAAGAACACATGAATGGT+TGG | - | chr1.4:40282037-40282056 | None:intergenic | 40.0% | |
| GTAGAGAACAATACCGATGA+AGG | + | chr1.4:40282489-40282508 | MS.gene90006:CDS | 40.0% | |
| GTGAAGATCAATGAACAAGG+TGG | + | chr1.4:40282360-40282379 | MS.gene90006:CDS | 40.0% | |
| TACCGATGAAGGTGATGAAA+CGG | + | chr1.4:40282500-40282519 | MS.gene90006:CDS | 40.0% | |
| TGAATCACCGTGACTGTAAA+AGG | + | chr1.4:40282304-40282323 | MS.gene90006:CDS | 40.0% | |
| TGTGCTAGAGAATTCACAAC+TGG | + | chr1.4:40281910-40281929 | MS.gene90006:CDS | 40.0% | |
| TTGGTCTCGTATCTTATTGG+TGG | - | chr1.4:40282189-40282208 | None:intergenic | 40.0% | |
| ! | CAATGAAAAGAACAGGTTGG+TGG | + | chr1.4:40282389-40282408 | MS.gene90006:CDS | 40.0% |
| ! | TACCCTTCTTCGAACCATTT+TGG | - | chr1.4:40282113-40282132 | None:intergenic | 40.0% |
| !! | TGGTGCTATGGAATTGTTTG+TGG | - | chr1.4:40281951-40281970 | None:intergenic | 40.0% |
| ACATTGTCATCATTGGAGGC+AGG | - | chr1.4:40282471-40282490 | None:intergenic | 45.0% | |
| ATAATCCCAGCTCCGTTTCT+TGG | - | chr1.4:40282208-40282227 | None:intergenic | 45.0% | |
| CAGCCAAAATGGTTCGAAGA+AGG | + | chr1.4:40282107-40282126 | MS.gene90006:CDS | 45.0% | |
| GATGAAGGTGATGAAACGGA+TGG | + | chr1.4:40282504-40282523 | MS.gene90006:CDS | 45.0% | |
| TGAAGGTGATGAAACGGATG+GGG | + | chr1.4:40282506-40282525 | MS.gene90006:CDS | 45.0% | |
| ! | TTCACAACTGGTAATGCCCT+CGG | + | chr1.4:40281922-40281941 | MS.gene90006:CDS | 45.0% |
| ACGAGACCAAGAAACGGAGC+TGG | + | chr1.4:40282199-40282218 | MS.gene90006:CDS | 55.0% | |
| CGAGACCAAGAAACGGAGCT+GGG | + | chr1.4:40282200-40282219 | MS.gene90006:CDS | 55.0% | |
| GAATTGTTTGTGGCCGCCGA+GGG | - | chr1.4:40281941-40281960 | None:intergenic | 55.0% | |
| ! | ACAACTGGTAATGCCCTCGG+CGG | + | chr1.4:40281925-40281944 | MS.gene90006:CDS | 55.0% |
| GGAATTGTTTGTGGCCGCCG+AGG | - | chr1.4:40281942-40281961 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 40281778 | 40282620 | 40281778 | ID=MS.gene90006 |
| chr1.4 | mRNA | 40281778 | 40282620 | 40281778 | ID=MS.gene90006.t1;Parent=MS.gene90006 |
| chr1.4 | exon | 40281778 | 40282620 | 40281778 | ID=MS.gene90006.t1.exon1;Parent=MS.gene90006.t1 |
| chr1.4 | CDS | 40281778 | 40282620 | 40281778 | ID=cds.MS.gene90006.t1;Parent=MS.gene90006.t1 |
| Gene Sequence |
| Protein sequence |
