Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90032.t1 | XP_013447966.1 | 89.5 | 220 | 23 | 0 | 1 | 220 | 1 | 220 | 1.20E-98 | 369.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90032.t1 | Q39261 | 42.1 | 76 | 37 | 2 | 83 | 151 | 52 | 127 | 4.0e-07 | 56.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90032.t1 | A0A072TYR6 | 89.5 | 220 | 23 | 0 | 1 | 220 | 1 | 220 | 8.4e-99 | 369.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene90032.t1 | TF | C2H2 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90032.t1 | MTR_7g028890 | 89.545 | 220 | 23 | 0 | 1 | 220 | 1 | 220 | 1.46e-143 | 399 |
| MS.gene90032.t1 | MTR_7g022250 | 74.477 | 239 | 42 | 2 | 1 | 220 | 1 | 239 | 2.81e-125 | 354 |
| MS.gene90032.t1 | MTR_7g028915 | 71.795 | 234 | 30 | 2 | 1 | 220 | 1 | 212 | 2.11e-112 | 320 |
| MS.gene90032.t1 | MTR_7g022270 | 58.791 | 182 | 53 | 4 | 20 | 200 | 1 | 161 | 5.34e-69 | 209 |
| MS.gene90032.t1 | MTR_4g128340 | 30.714 | 140 | 78 | 4 | 83 | 220 | 47 | 169 | 8.84e-12 | 61.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 38 sgRNAs with CRISPR-Local
Find 40 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGAAATGGAGAAATTTCTT+TGG | 0.293893 | 7.4:-72984016 | None:intergenic |
| CTCCTTCCTCTTGTTGCACA+TGG | 0.338528 | 7.4:-72983836 | None:intergenic |
| TATATATAAATCAAAGCTTA+AGG | 0.356106 | 7.4:-72984488 | None:intergenic |
| GGGTGTGGATATCAATATAA+TGG | 0.359691 | 7.4:+72984222 | MS.gene90032:CDS |
| TGGTGCATTGCAACATATTC+CGG | 0.362580 | 7.4:+72984302 | MS.gene90032:CDS |
| CACCATCACTCCATTTCATA+TGG | 0.364608 | 7.4:+72984200 | MS.gene90032:CDS |
| CTTAAGGGTCAAATCAATCT+TGG | 0.396784 | 7.4:-72984472 | None:intergenic |
| AACAATATGATTCAACATGC+AGG | 0.449556 | 7.4:+72984249 | MS.gene90032:CDS |
| TATCCACACCCCATATGAAA+TGG | 0.452870 | 7.4:-72984210 | None:intergenic |
| AGTAGACAATTTGAAGATGA+TGG | 0.466141 | 7.4:+72983876 | MS.gene90032:CDS |
| TTCACTAGTCCACAGGCATT+AGG | 0.474635 | 7.4:+72984111 | MS.gene90032:CDS |
| TTGGATTCATTGTTGATCAC+AGG | 0.479894 | 7.4:+72984043 | MS.gene90032:CDS |
| TGAAGTTGGTAAAGTTTCAG+AGG | 0.484639 | 7.4:+72984389 | MS.gene90032:CDS |
| ACTATGTCAAAGATTGAAGT+TGG | 0.488225 | 7.4:+72984375 | MS.gene90032:CDS |
| ACCATCACTCCATTTCATAT+GGG | 0.489513 | 7.4:+72984201 | MS.gene90032:CDS |
| TGGTGTTTAGACTTATCCCT+TGG | 0.491881 | 7.4:-72984182 | None:intergenic |
| TGTTAGTGATGAAGTTGATC+AGG | 0.506770 | 7.4:+72984419 | MS.gene90032:CDS |
| CAAGCGAAAGGCAATTGTGC+TGG | 0.510108 | 7.4:+72983918 | MS.gene90032:CDS |
| GAAGTTGGTAAAGTTTCAGA+GGG | 0.513957 | 7.4:+72984390 | MS.gene90032:CDS |
| ATATATAAATCAAAGCTTAA+GGG | 0.520066 | 7.4:-72984487 | None:intergenic |
| ACTCCATTTCATATGGGGTG+TGG | 0.533278 | 7.4:+72984207 | MS.gene90032:CDS |
| GGAGATAGATGAAGCGAATG+AGG | 0.566081 | 7.4:+72984323 | MS.gene90032:CDS |
| TCATTCGCTTCATCTATCTC+CGG | 0.569516 | 7.4:-72984321 | None:intergenic |
| CAGAAAGTTCACTAGTCCAC+AGG | 0.575345 | 7.4:+72984104 | MS.gene90032:CDS |
| ACAATATGATTCAACATGCA+GGG | 0.581032 | 7.4:+72984250 | MS.gene90032:CDS |
| CTGATGCCATGTGCAACAAG+AGG | 0.598560 | 7.4:+72983830 | None:intergenic |
| TAGAATTCTGAGTTTCAACA+TGG | 0.602831 | 7.4:-72983948 | None:intergenic |
| TGCCATGTGCAACAAGAGGA+AGG | 0.602996 | 7.4:+72983834 | None:intergenic |
| GTTGGTAAAGTTTCAGAGGG+AGG | 0.624522 | 7.4:+72984393 | MS.gene90032:CDS |
| CCATCACTCCATTTCATATG+GGG | 0.630153 | 7.4:+72984202 | MS.gene90032:CDS |
| GGAAGACAAAGAGACTCAAG+AGG | 0.634668 | 7.4:+72984440 | MS.gene90032:CDS |
| GTGCAACAAGAGGAAGGAGA+AGG | 0.635140 | 7.4:+72983840 | MS.gene90032:CDS |
| CCCCATATGAAATGGAGTGA+TGG | 0.640487 | 7.4:-72984202 | None:intergenic |
| GAACAATGAGAACAAGCGAA+AGG | 0.645636 | 7.4:+72983906 | MS.gene90032:CDS |
| ACTAGTCCACAGGCATTAGG+TGG | 0.661075 | 7.4:+72984114 | MS.gene90032:CDS |
| TGATGACCACCTAATGCCTG+TGG | 0.664402 | 7.4:-72984120 | None:intergenic |
| AGAATTCTGAGTTTCAACAT+GGG | 0.673130 | 7.4:-72983947 | None:intergenic |
| AGACAAAGAGACTCAAGAGG+AGG | 0.673262 | 7.4:+72984443 | MS.gene90032:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAGAAATGGAGAAATTTCTT+TGG | - | chr7.4:72984019-72984038 | None:intergenic | 25.0% |
| ! | ATGAATCCAAAAAGAAGAAA+TGG | - | chr7.4:72984033-72984052 | None:intergenic | 25.0% |
| !! | ATTTCTCCATTTCTTCTTTT+TGG | + | chr7.4:72984024-72984043 | MS.gene90032:CDS | 25.0% |
| !! | TTTTATGCAGGAAATTTGTA+TGG | + | chr7.4:72984282-72984301 | MS.gene90032:CDS | 25.0% |
| AACAATATGATTCAACATGC+AGG | + | chr7.4:72984249-72984268 | MS.gene90032:CDS | 30.0% | |
| ACAATATGATTCAACATGCA+GGG | + | chr7.4:72984250-72984269 | MS.gene90032:CDS | 30.0% | |
| ACTATGTCAAAGATTGAAGT+TGG | + | chr7.4:72984375-72984394 | MS.gene90032:CDS | 30.0% | |
| AGAATTCTGAGTTTCAACAT+GGG | - | chr7.4:72983950-72983969 | None:intergenic | 30.0% | |
| AGTAGACAATTTGAAGATGA+TGG | + | chr7.4:72983876-72983895 | MS.gene90032:CDS | 30.0% | |
| AGTCTCAATAAAAAGATCCA+AGG | + | chr7.4:72984165-72984184 | MS.gene90032:CDS | 30.0% | |
| GTCTCAATAAAAAGATCCAA+GGG | + | chr7.4:72984166-72984185 | MS.gene90032:CDS | 30.0% | |
| TAGAATTCTGAGTTTCAACA+TGG | - | chr7.4:72983951-72983970 | None:intergenic | 30.0% | |
| ACCATCACTCCATTTCATAT+GGG | + | chr7.4:72984201-72984220 | MS.gene90032:CDS | 35.0% | |
| CTTAAGGGTCAAATCAATCT+TGG | - | chr7.4:72984475-72984494 | None:intergenic | 35.0% | |
| GGGTGTGGATATCAATATAA+TGG | + | chr7.4:72984222-72984241 | MS.gene90032:CDS | 35.0% | |
| TTGGATTCATTGTTGATCAC+AGG | + | chr7.4:72984043-72984062 | MS.gene90032:CDS | 35.0% | |
| ! | CTTCTTCAGCAACATCTTTT+TGG | - | chr7.4:72983981-72984000 | None:intergenic | 35.0% |
| ! | TGTTAGTGATGAAGTTGATC+AGG | + | chr7.4:72984419-72984438 | MS.gene90032:CDS | 35.0% |
| !! | GAAGTTGGTAAAGTTTCAGA+GGG | + | chr7.4:72984390-72984409 | MS.gene90032:CDS | 35.0% |
| !! | TGAAGTTGGTAAAGTTTCAG+AGG | + | chr7.4:72984389-72984408 | MS.gene90032:CDS | 35.0% |
| CACCATCACTCCATTTCATA+TGG | + | chr7.4:72984200-72984219 | MS.gene90032:CDS | 40.0% | |
| CCATCACTCCATTTCATATG+GGG | + | chr7.4:72984202-72984221 | MS.gene90032:CDS | 40.0% | |
| GAACAATGAGAACAAGCGAA+AGG | + | chr7.4:72983906-72983925 | MS.gene90032:CDS | 40.0% | |
| TATCCACACCCCATATGAAA+TGG | - | chr7.4:72984213-72984232 | None:intergenic | 40.0% | |
| TCATTCGCTTCATCTATCTC+CGG | - | chr7.4:72984324-72984343 | None:intergenic | 40.0% | |
| TGGTGCATTGCAACATATTC+CGG | + | chr7.4:72984302-72984321 | MS.gene90032:CDS | 40.0% | |
| TGGTGTTTAGACTTATCCCT+TGG | - | chr7.4:72984185-72984204 | None:intergenic | 40.0% | |
| ! | GGGTCATCAAACTTTTATGC+AGG | + | chr7.4:72984270-72984289 | MS.gene90032:CDS | 40.0% |
| ACTCCATTTCATATGGGGTG+TGG | + | chr7.4:72984207-72984226 | MS.gene90032:CDS | 45.0% | |
| AGACAAAGAGACTCAAGAGG+AGG | + | chr7.4:72984443-72984462 | MS.gene90032:CDS | 45.0% | |
| CAGAAAGTTCACTAGTCCAC+AGG | + | chr7.4:72984104-72984123 | MS.gene90032:CDS | 45.0% | |
| CCCCATATGAAATGGAGTGA+TGG | - | chr7.4:72984205-72984224 | None:intergenic | 45.0% | |
| GGAAGACAAAGAGACTCAAG+AGG | + | chr7.4:72984440-72984459 | MS.gene90032:CDS | 45.0% | |
| GGAGATAGATGAAGCGAATG+AGG | + | chr7.4:72984323-72984342 | MS.gene90032:CDS | 45.0% | |
| TTCACTAGTCCACAGGCATT+AGG | + | chr7.4:72984111-72984130 | MS.gene90032:CDS | 45.0% | |
| ! | GTTGGTAAAGTTTCAGAGGG+AGG | + | chr7.4:72984393-72984412 | MS.gene90032:CDS | 45.0% |
| ACTAGTCCACAGGCATTAGG+TGG | + | chr7.4:72984114-72984133 | MS.gene90032:CDS | 50.0% | |
| CAAGCGAAAGGCAATTGTGC+TGG | + | chr7.4:72983918-72983937 | MS.gene90032:CDS | 50.0% | |
| GTGCAACAAGAGGAAGGAGA+AGG | + | chr7.4:72983840-72983859 | MS.gene90032:CDS | 50.0% | |
| TGATGACCACCTAATGCCTG+TGG | - | chr7.4:72984123-72984142 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 72983838 | 72984500 | 72983838 | ID=MS.gene90032 |
| chr7.4 | mRNA | 72983838 | 72984500 | 72983838 | ID=MS.gene90032.t1;Parent=MS.gene90032 |
| chr7.4 | exon | 72983838 | 72984500 | 72983838 | ID=MS.gene90032.t1.exon1;Parent=MS.gene90032.t1 |
| chr7.4 | CDS | 72983838 | 72984500 | 72983838 | ID=cds.MS.gene90032.t1;Parent=MS.gene90032.t1 |
| Gene Sequence |
| Protein sequence |
