AlfalfaGEDB | MS.gene90278

M. sativa cultivar XinJiangDaYe / MS.gene90278

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0046983 GO:0097159 GO:1901363
PFAM: PF00319.21

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene90278.t1	XP_003627433.1	85.8	183	24	2	1	181	1	183	1.60E-74	288.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene90278.t1	Q9FKK2	29.5	156	105	1	15	165	5	160	8.1e-14	78.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene90278.t1	G7L955	85.8	183	24	2	1	181	1	183	1.2e-74	288.9

TFs/TRs:

Gene ID	Type	Classification
MS.gene90278.t1	TF	MADS-M-type

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene90278.t1	MTR_8g022970	91.257	183	14	2	1	181	1	183	5.54e-118	332
MS.gene90278.t1	MTR_3g109930	73.125	160	43	0	22	181	7	166	1.14e-79	234
MS.gene90278.t1	MTR_3g437790	48.023	177	79	6	10	179	8	178	1.77e-41	138
MS.gene90278.t1	MTR_2g045020	45.506	178	84	5	3	176	8	176	6.39e-36	124
MS.gene90278.t1	MTR_7g028448	35.366	164	91	3	14	172	4	157	1.14e-24	94.7
MS.gene90278.t1	MTR_4g036915	35.366	164	91	3	14	172	4	157	1.14e-24	94.7
MS.gene90278.t1	MTR_2g105290	35.714	154	97	2	15	168	10	161	5.50e-24	93.2
MS.gene90278.t1	MTR_1g012570	34.356	163	95	3	10	165	2	159	6.36e-21	86.7
MS.gene90278.t1	MTR_1g106070	30.723	166	97	3	10	165	2	159	1.01e-18	79.7
MS.gene90278.t1	MTR_3g052920	31.176	170	99	3	10	169	2	163	1.02e-18	80.5
MS.gene90278.t1	MTR_8g086290	33.117	154	101	2	15	168	10	161	1.23e-18	79.3
MS.gene90278.t1	MTR_8g043650	32.051	156	100	3	10	161	2	155	1.33e-18	80.5
MS.gene90278.t1	MTR_1g105910	32.075	159	96	3	10	161	2	155	1.53e-18	80.1
MS.gene90278.t1	MTR_2g030740	32.500	160	102	3	10	165	2	159	1.62e-18	80.1
MS.gene90278.t1	MTR_5g053390	34.969	163	94	4	10	165	2	159	2.08e-18	79.7
MS.gene90278.t1	MTR_1g105920	30.120	166	98	3	10	165	2	159	2.30e-18	79.7
MS.gene90278.t1	MTR_1g108510	31.325	166	96	4	10	165	2	159	3.98e-18	79.0
MS.gene90278.t1	MTR_2g085250	32.500	160	90	4	16	165	8	159	4.25e-18	79.0
MS.gene90278.t1	MTR_2g085280	32.500	160	90	4	16	165	8	159	4.39e-18	79.0
MS.gene90278.t1	MTR_3g030770	31.325	166	96	4	10	165	2	159	6.56e-18	78.2
MS.gene90278.t1	MTR_5g055100	33.951	162	95	3	10	164	2	158	6.65e-18	78.6
MS.gene90278.t1	MTR_5g045560	32.903	155	92	3	18	165	10	159	9.33e-17	75.5
MS.gene90278.t1	MTR_1g108580	30.723	166	97	4	10	165	2	159	1.17e-16	75.1
MS.gene90278.t1	MTR_0121s0080	34.177	158	80	5	21	165	13	159	2.64e-16	74.3
MS.gene90278.t1	MTR_1g075600	30.952	168	94	5	10	165	2	159	2.70e-16	74.3
MS.gene90278.t1	MTR_3g080940	28.750	160	98	3	18	168	10	162	2.89e-16	73.6
MS.gene90278.t1	MTR_4g127140	31.469	143	88	2	35	168	30	171	1.75e-15	71.2
MS.gene90278.t1	MTR_1g105905	32.117	137	81	3	35	164	10	141	1.84e-15	71.6
MS.gene90278.t1	MTR_1g077360	31.515	165	97	5	10	165	2	159	3.40e-15	71.2
MS.gene90278.t1	MTR_3g052870	39.744	78	47	0	10	87	2	79	2.67e-14	68.6
MS.gene90278.t1	MTR_1g047550	38.462	78	48	0	18	95	3	80	6.41e-14	66.6
MS.gene90278.t1	MTR_8g079502	40.845	71	42	0	18	88	3	73	1.07e-13	66.2
MS.gene90278.t1	MTR_2g093190	30.000	160	106	3	10	165	2	159	1.14e-13	67.0
MS.gene90278.t1	MTR_4g131030	42.254	71	41	0	18	88	3	73	4.70e-13	65.1
MS.gene90278.t1	MTR_8g046350	33.962	106	62	2	18	120	3	103	5.97e-13	63.9
MS.gene90278.t1	MTR_8g051580	42.254	71	41	0	18	88	3	73	6.51e-13	63.9
MS.gene90278.t1	MTR_1g054265	42.647	68	39	0	18	85	3	70	8.31e-13	63.5
MS.gene90278.t1	MTR_7g062350	33.099	142	75	4	35	165	39	171	8.88e-13	64.7
MS.gene90278.t1	MTR_1g063160	38.298	94	57	1	18	110	3	96	1.50e-12	63.2
MS.gene90278.t1	MTR_3g030780	38.235	68	42	0	18	85	10	77	9.41e-12	60.1
MS.gene90278.t1	MTR_0121s0100	38.158	76	47	0	10	85	2	77	1.21e-11	59.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene90278.t1	AT5G60440	29.677	155	104	1	16	165	6	160	9.01e-18	79.3
MS.gene90278.t1	AT2G24840	27.815	151	106	2	18	165	64	214	2.66e-15	72.0
MS.gene90278.t1	AT2G34440	29.341	167	104	3	18	179	3	160	3.51e-15	70.1
MS.gene90278.t1	AT3G66656	43.284	67	38	0	18	84	3	69	1.53e-14	68.6
MS.gene90278.t1	AT1G65360	46.269	67	36	0	18	84	8	74	1.88e-14	68.9
MS.gene90278.t1	AT1G01530	31.902	163	96	4	15	169	5	160	5.36e-14	68.2
MS.gene90278.t1	AT3G04100	27.841	176	111	3	3	168	2	171	4.52e-12	62.4

Find 19 sgRNAs with CRISPR-Local

Find 32 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTGCCTCATGTTGAAGTCTT+AGG	0.146373	8.2:-72897387	None:intergenic
GGTTCATTCTATTGATGATA+TGG	0.250456	8.2:+72897495	MS.gene90278:CDS
TGAGCAATTTAAGACTTCTT+TGG	0.270286	8.2:+72897531	MS.gene90278:CDS
GAAGAGAATAATTATTTAGA+AGG	0.328216	8.2:+72897427	MS.gene90278:CDS
TTCTATTGATGATATGGCTT+TGG	0.335359	8.2:+72897501	MS.gene90278:CDS
AAGTGTTTAATGACAGTGTT+GGG	0.346748	8.2:-72897298	None:intergenic
TTAATGACAGTGTTGGGGTT+AGG	0.355771	8.2:-72897292	None:intergenic
TAATGACAGTGTTGGGGTTA+GGG	0.369809	8.2:-72897291	None:intergenic
TGCCTCATGTTGAAGTCTTA+GGG	0.391433	8.2:-72897386	None:intergenic
CATTAAACACTTTCTCGATA+AGG	0.401846	8.2:+72897309	MS.gene90278:CDS
GCTTAATTTAGATGCATCCT+TGG	0.469345	8.2:+72897564	MS.gene90278:CDS
GATCCAATGGCTTCTTCAGA+TGG	0.481889	8.2:+72897064	None:intergenic
TTTCCATCTGAAGAAGCCAT+TGG	0.494665	8.2:-72897067	None:intergenic
AATTTAGATGCATCCTTGGA+AGG	0.508160	8.2:+72897568	MS.gene90278:CDS
AAAGTGTTTAATGACAGTGT+TGG	0.535573	8.2:-72897299	None:intergenic
ACCTTCTCAAAACGAAAACT+AGG	0.575975	8.2:+72897172	MS.gene90278:CDS
AACATGAGGCAATTGAAGAA+CGG	0.617681	8.2:+72897398	MS.gene90278:CDS
AGTGTTTAATGACAGTGTTG+GGG	0.636928	8.2:-72897297	None:intergenic
AACCCTAAGACTTCAACATG+AGG	0.703246	8.2:+72897384	MS.gene90278:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	GAAGAGAATAATTATTTAGA+AGG	+	chr8.2:72897427-72897446	MS.gene90278:CDS	20.0%
!!	TTCTGCTTTCTTGATTTTAA+TGG	-	chr8.2:72897131-72897150	None:intergenic	25.0%
	AAGTGTTTAATGACAGTGTT+GGG	-	chr8.2:72897301-72897320	None:intergenic	30.0%
	CATTAAACACTTTCTCGATA+AGG	+	chr8.2:72897309-72897328	MS.gene90278:CDS	30.0%
!	AAAGTGTTTAATGACAGTGT+TGG	-	chr8.2:72897302-72897321	None:intergenic	30.0%
!	GGTTCATTCTATTGATGATA+TGG	+	chr8.2:72897495-72897514	MS.gene90278:CDS	30.0%
!	TGAGCAATTTAAGACTTCTT+TGG	+	chr8.2:72897531-72897550	MS.gene90278:CDS	30.0%
!	TTCTATTGATGATATGGCTT+TGG	+	chr8.2:72897501-72897520	MS.gene90278:CDS	30.0%
!!	GTAGTTCAAGTTTTTCTTGT+TGG	+	chr8.2:72897470-72897489	MS.gene90278:CDS	30.0%
!!	TTCAAGTTTTTCTTGTTGGT+GGG	+	chr8.2:72897474-72897493	MS.gene90278:CDS	30.0%
	AACATGAGGCAATTGAAGAA+CGG	+	chr8.2:72897398-72897417	MS.gene90278:CDS	35.0%
	AACGAAAAACTCTACGTTTG+TGG	+	chr8.2:72897265-72897284	MS.gene90278:CDS	35.0%
	AATTTAGATGCATCCTTGGA+AGG	+	chr8.2:72897568-72897587	MS.gene90278:CDS	35.0%
	ACCTTCTCAAAACGAAAACT+AGG	+	chr8.2:72897172-72897191	MS.gene90278:CDS	35.0%
	AGTGTTTAATGACAGTGTTG+GGG	-	chr8.2:72897300-72897319	None:intergenic	35.0%
	GAAAAAAGGAAGCAAGATGA+GGG	+	chr8.2:72897352-72897371	MS.gene90278:CDS	35.0%
	GCTTAATTTAGATGCATCCT+TGG	+	chr8.2:72897564-72897583	MS.gene90278:CDS	35.0%
	TGAAAAAAGGAAGCAAGATG+AGG	+	chr8.2:72897351-72897370	MS.gene90278:CDS	35.0%
!	ATGAATCTTTTTGCCTTCCA+AGG	-	chr8.2:72897584-72897603	None:intergenic	35.0%
!	CAAACGTAGAGTTTTTCGTT+TGG	-	chr8.2:72897265-72897284	None:intergenic	35.0%
!!	GTTCAAGTTTTTCTTGTTGG+TGG	+	chr8.2:72897473-72897492	MS.gene90278:CDS	35.0%
!!!	TCCTAGTTTTCGTTTTGAGA+AGG	-	chr8.2:72897176-72897195	None:intergenic	35.0%
!!!	TTCGTTTTGAGAAGGTATCA+CGG	-	chr8.2:72897168-72897187	None:intergenic	35.0%
	AACCCTAAGACTTCAACATG+AGG	+	chr8.2:72897384-72897403	MS.gene90278:CDS	40.0%
	TAGTCATCGACGCTGAAAAA+AGG	+	chr8.2:72897338-72897357	MS.gene90278:CDS	40.0%
	TGCCTCATGTTGAAGTCTTA+GGG	-	chr8.2:72897389-72897408	None:intergenic	40.0%
	TTGCCTCATGTTGAAGTCTT+AGG	-	chr8.2:72897390-72897409	None:intergenic	40.0%
!	TAATGACAGTGTTGGGGTTA+GGG	-	chr8.2:72897294-72897313	None:intergenic	40.0%
!	TTAATGACAGTGTTGGGGTT+AGG	-	chr8.2:72897295-72897314	None:intergenic	40.0%
!!!	TCGTTTTGAGAAGGTATCAC+GGG	-	chr8.2:72897167-72897186	None:intergenic	40.0%
!!!	TTTTTTCAGCGTCGATGACT+AGG	-	chr8.2:72897339-72897358	None:intergenic	40.0%
!!!	CGTTTTGAGAAGGTATCACG+GGG	-	chr8.2:72897166-72897185	None:intergenic	45.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.2	gene	72897070	72897615	72897070	ID=MS.gene90278
chr8.2	mRNA	72897070	72897615	72897070	ID=MS.gene90278.t1;Parent=MS.gene90278
chr8.2	exon	72897070	72897615	72897070	ID=MS.gene90278.t1.exon1;Parent=MS.gene90278.t1
chr8.2	CDS	72897070	72897615	72897070	ID=cds.MS.gene90278.t1;Parent=MS.gene90278.t1

Gene Sequence

>MS.gene90278

ATGGCTTCTTCAGATGGAAACAACAAATCAACAAAAAGCAACAACAAACGTAAGAGAACCATTAAAATCAAGAAAGCAGAACAATCAAACAATCCCCGTGATACCTTCTCAAAACGAAAACTAGGACTCTTCAACAAAGTTACAGAACTATCTATTCTATGCAATGCAAAAATTGCAATGATCATCACTTCACCAAACGAAAAACTCTACGTTTGTGGTTACCCTAACCCCAACACTGTCATTAAACACTTTCTCGATAAGGAAAACCTAGTCATCGACGCTGAAAAAAGGAAGCAAGATGAGGGCGTAGTTGAAACCCTAAGACTTCAACATGAGGCAATTGAAGAACGGCTGAAAGAAGAGAATAATTATTTAGAAGGTGTAAAAGAGAGAAACAAGAGTAGTTCAAGTTTTTCTTGTTGGTGGGTTCATTCTATTGATGATATGGCTTTGGAATCTCTTGAGCAATTTAAGACTTCTTTGGTAAAATTGAAGCTTAATTTAGATGCATCCTTGGAAGGCAAAAAGATTCATATAACTACTTAA

Protein sequence

>MS.gene90278.t1

MASSDGNNKSTKSNNKRKRTIKIKKAEQSNNPRDTFSKRKLGLFNKVTELSILCNAKIAMIITSPNEKLYVCGYPNPNTVIKHFLDKENLVIDAEKRKQDEGVVETLRLQHEAIEERLKEENNYLEGVKERNKSSSSFSCWWVHSIDDMALESLEQFKTSLVKLKLNLDASLEGKKIHITT