AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene90350

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene90350.t1 MTR_7g059400 92.373 354 21 2 1 354 1 348 0.0 555
MS.gene90350.t1 MTR_4g022370 48.438 384 124 17 1 341 1 353 3.04e-86 265
MS.gene90350.t1 MTR_4g063780 43.064 346 166 9 1 330 1 331 1.51e-72 229
MS.gene90350.t1 MTR_2g096740 40.357 280 107 10 1 260 1 240 4.20e-50 170
MS.gene90350.t1 MTR_7g024670 55.479 146 57 4 1 142 1 142 1.65e-44 157
MS.gene90350.t1 MTR_2g096740 40.076 262 118 11 1 260 1 225 4.33e-44 154
MS.gene90350.t1 MTR_8g479350 56.000 100 38 1 77 170 69 168 1.48e-31 122
MS.gene90350.t1 MTR_3g090430 63.750 80 29 0 57 136 32 111 1.37e-30 119
MS.gene90350.t1 MTR_3g091820 66.667 75 21 1 57 131 28 98 7.47e-30 116
MS.gene90350.t1 MTR_5g031440 59.633 109 39 1 73 181 40 143 1.31e-29 116
MS.gene90350.t1 MTR_3g077750 63.636 88 25 3 43 126 27 111 1.62e-29 116
MS.gene90350.t1 MTR_2g093220 78.689 61 13 0 70 130 34 94 1.93e-29 115
MS.gene90350.t1 MTR_8g068210 73.438 64 17 0 72 135 28 91 2.82e-29 115
MS.gene90350.t1 MTR_4g461080 81.356 59 11 0 70 128 23 81 3.61e-29 114
MS.gene90350.t1 MTR_8g079060 60.000 105 25 4 55 151 17 112 1.11e-28 111
MS.gene90350.t1 MTR_2g014060 67.606 71 22 1 58 127 6 76 1.71e-28 113
MS.gene90350.t1 MTR_4g109980 90.000 50 5 0 77 126 41 90 5.76e-28 111
MS.gene90350.t1 MTR_8g015840 73.913 69 15 2 68 136 13 78 7.13e-28 108
MS.gene90350.t1 MTR_8g027295 71.014 69 16 1 78 142 26 94 8.23e-28 110
MS.gene90350.t1 MTR_1g056810 80.357 56 11 0 75 130 11 66 8.31e-28 110
MS.gene90350.t1 MTR_2g014170 89.583 48 5 0 77 124 46 93 1.31e-26 107
MS.gene90350.t1 MTR_2g013370 65.278 72 16 2 69 132 31 101 2.26e-26 106
MS.gene90350.t1 MTR_4g089095 81.818 55 9 1 78 132 52 105 2.53e-26 106
MS.gene90350.t1 MTR_4g089095 81.818 55 9 1 78 132 18 71 2.86e-26 105
MS.gene90350.t1 MTR_1g077600 52.439 82 37 1 53 132 18 99 4.71e-26 105
MS.gene90350.t1 MTR_1g077600 52.439 82 37 1 53 132 18 99 5.78e-26 105
MS.gene90350.t1 MTR_2g059540 87.500 48 6 0 77 124 32 79 1.33e-25 102
MS.gene90350.t1 MTR_6g012450 71.429 56 16 0 78 133 138 193 3.35e-23 100
MS.gene90350.t1 MTR_7g086780 42.982 114 54 1 77 179 100 213 3.74e-23 100
MS.gene90350.t1 MTR_3g435480 68.421 57 18 0 78 134 138 194 5.97e-23 99.8
MS.gene90350.t1 MTR_7g010950 61.194 67 26 0 78 144 137 203 8.04e-23 99.8
MS.gene90350.t1 MTR_2g016030 53.247 77 33 1 78 154 49 122 9.29e-23 93.6
MS.gene90350.t1 MTR_8g044220 68.519 54 17 0 78 131 120 173 1.01e-22 99.0
MS.gene90350.t1 MTR_6g027450 66.667 54 18 0 77 130 90 143 6.07e-22 95.5
MS.gene90350.t1 MTR_4g082060 69.231 52 16 0 78 129 124 175 6.72e-22 96.7
MS.gene90350.t1 MTR_5g041420 61.290 62 24 0 78 139 140 201 2.42e-21 93.6
MS.gene90350.t1 MTR_5g041400 58.667 75 25 1 78 152 121 189 2.52e-21 94.0
MS.gene90350.t1 MTR_5g041380 66.102 59 20 0 78 136 129 187 9.29e-21 92.4
MS.gene90350.t1 MTR_6g027460 57.377 61 24 1 71 129 89 149 1.27e-20 92.0
MS.gene90350.t1 MTR_5g041530 69.231 52 16 0 78 129 142 193 1.81e-20 92.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene90350.t1 AT3G55370 38.220 382 156 18 1 339 1 345 2.45e-52 177
MS.gene90350.t1 AT3G55370 38.630 365 147 17 1 339 1 314 2.32e-51 174
MS.gene90350.t1 AT3G55370 40.514 311 123 14 48 339 92 359 2.33e-46 162
MS.gene90350.t1 AT2G37590 40.217 276 108 9 1 240 1 255 4.30e-45 157
MS.gene90350.t1 AT5G02460 86.842 76 10 0 55 130 74 149 7.69e-44 156
MS.gene90350.t1 AT1G07640 73.418 79 21 0 58 136 3 81 1.01e-36 134
MS.gene90350.t1 AT1G07640 73.418 79 21 0 58 136 59 137 1.57e-36 135
MS.gene90350.t1 AT1G07640 73.418 79 21 0 58 136 67 145 1.99e-36 135
MS.gene90350.t1 AT2G28810 46.711 152 49 6 1 127 1 145 2.21e-34 129
MS.gene90350.t1 AT5G60200 69.512 82 22 1 73 151 52 133 1.70e-33 125
MS.gene90350.t1 AT5G60850 61.957 92 32 1 63 154 38 126 3.35e-32 122
MS.gene90350.t1 AT2G28810 74.648 71 17 1 58 127 58 128 3.36e-32 123
MS.gene90350.t1 AT3G52440 72.464 69 19 0 73 141 22 90 4.57e-31 118
MS.gene90350.t1 AT3G52440 72.464 69 19 0 73 141 41 109 7.02e-31 118
MS.gene90350.t1 AT3G45610 68.571 70 22 0 73 142 37 106 1.35e-30 117
MS.gene90350.t1 AT3G21270 49.635 137 56 3 77 213 30 153 4.68e-30 114
MS.gene90350.t1 AT1G51700 67.500 80 24 2 69 147 27 105 4.83e-30 114
MS.gene90350.t1 AT5G65590 54.639 97 37 2 35 131 7 96 6.57e-30 117
MS.gene90350.t1 AT1G28310 83.929 56 9 0 77 132 28 83 2.86e-29 115
MS.gene90350.t1 AT1G28310 83.929 56 9 0 77 132 52 107 4.71e-29 115
MS.gene90350.t1 AT1G21340 61.176 85 31 1 78 162 40 122 6.68e-29 112
MS.gene90350.t1 AT4G21050 40.476 168 72 4 74 213 22 189 8.06e-29 111
MS.gene90350.t1 AT5G62940 47.656 128 60 4 1 126 1 123 6.12e-28 112
MS.gene90350.t1 AT5G66940 51.351 111 42 4 37 145 2 102 9.60e-28 108
MS.gene90350.t1 AT3G61850 45.985 137 55 5 10 133 1 131 9.96e-28 110
MS.gene90350.t1 AT3G50410 56.818 88 35 2 49 133 1 88 1.09e-27 109
MS.gene90350.t1 AT4G38000 61.446 83 31 1 70 152 35 116 1.22e-27 109
MS.gene90350.t1 AT3G61850 54.455 101 41 3 38 133 19 119 1.45e-27 110
MS.gene90350.t1 AT3G61850 54.455 101 41 3 38 133 19 119 1.45e-27 110
MS.gene90350.t1 AT3G61850 45.985 137 55 5 10 133 1 131 1.66e-27 110
MS.gene90350.t1 AT3G61850 54.455 101 41 3 38 133 19 119 2.19e-27 110
MS.gene90350.t1 AT4G24060 72.131 61 17 0 70 130 47 107 2.54e-27 110
MS.gene90350.t1 AT4G00940 74.576 59 14 1 72 130 65 122 6.27e-26 105
MS.gene90350.t1 AT4G00940 74.576 59 14 1 72 130 65 122 6.27e-26 105
MS.gene90350.t1 AT4G00940 74.576 59 14 1 72 130 65 122 6.27e-26 105
MS.gene90350.t1 AT2G28510 38.057 247 107 9 75 321 47 247 1.63e-25 104
MS.gene90350.t1 AT2G46590 77.358 53 10 1 73 123 63 115 9.06e-24 100
MS.gene90350.t1 AT2G46590 77.358 53 10 1 73 123 75 127 1.12e-23 100
MS.gene90350.t1 AT1G64620 47.020 151 59 5 70 213 43 179 1.19e-23 100
MS.gene90350.t1 AT5G62430 73.077 52 14 0 78 129 56 107 1.72e-23 99.0
MS.gene90350.t1 AT3G47500 63.636 66 24 0 78 143 112 177 2.75e-23 100
MS.gene90350.t1 AT1G47655 78.723 47 10 0 78 124 31 77 1.66e-22 94.4
MS.gene90350.t1 AT1G26790 69.231 52 16 0 78 129 113 164 3.03e-22 97.1
MS.gene90350.t1 AT2G34140 71.154 52 15 0 78 129 60 111 3.07e-22 92.4
MS.gene90350.t1 AT1G29160 71.154 52 15 0 78 129 64 115 3.26e-22 92.4
MS.gene90350.t1 AT4G21040 72.727 55 14 1 78 132 27 80 5.46e-22 93.6
MS.gene90350.t1 AT5G39660 71.154 52 15 0 78 129 140 191 1.06e-21 96.3
MS.gene90350.t1 AT5G39660 71.154 52 15 0 78 129 140 191 1.06e-21 96.3
MS.gene90350.t1 AT5G39660 71.154 52 15 0 78 129 140 191 1.06e-21 96.3
MS.gene90350.t1 AT1G69570 69.231 52 16 0 78 129 134 185 2.90e-21 94.4
MS.gene90350.t1 AT4G21080 53.165 79 33 1 78 156 27 101 7.84e-21 90.9
MS.gene90350.t1 AT4G21030 42.667 75 43 0 74 148 19 93 1.15e-13 69.3

Find 111 sgRNAs with CRISPR-Local

Find 130 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGCCAACCGGGACACTTCTA+AGG 0.192890 7.4:+44019331 None:intergenic
TACTTCTCTCCCACCATATA+TGG 0.222301 7.4:-44019831 MS.gene90350:CDS
TTCCAATATTGTTCACTATT+AGG 0.263046 7.4:+44018733 None:intergenic
TAGGTGGAGGCGTTGGTAAC+GGG 0.274562 7.4:-44019056 MS.gene90350:CDS
AAATAATTCTAGTGTGAAAA+TGG 0.280136 7.4:-44018827 MS.gene90350:CDS
ATCTCATCGGTGGTGGCGTT+GGG 0.300347 7.4:+44019193 None:intergenic
TTCCATATCGGAAGCTCTTT+AGG 0.302834 7.4:-44019075 MS.gene90350:CDS
GGTGATCACCTAAGTGAAAT+AGG 0.308942 7.4:-44019147 MS.gene90350:CDS
ATCCTAATAGTGAACAATAT+TGG 0.316935 7.4:-44018735 MS.gene90350:CDS
GAATCAATCTAGACAATTCT+TGG 0.322625 7.4:-44018800 MS.gene90350:CDS
GCAAGGCTTGTCGAAGGTAT+TGG 0.355195 7.4:-44019368 MS.gene90350:CDS
CACCTAAAGAGCTTCCGATA+TGG 0.360566 7.4:+44019073 None:intergenic
AGTTGTTTGTAGAGCTAGTT+TGG 0.376308 7.4:+44019235 None:intergenic
TGCATATGGTGGTGTGTCTA+AGG 0.387509 7.4:-44018857 MS.gene90350:CDS
CTTCGGCAACCACCGCCAAC+CGG 0.399525 7.4:+44019318 None:intergenic
CATGAGGTTGCGATGGTGTC+AGG 0.399816 7.4:+44019544 None:intergenic
ACCTGTTGATGCCAGTTGGC+TGG 0.401216 7.4:+44019806 None:intergenic
CAGTTGGCTGGATCCATATA+TGG 0.404780 7.4:+44019818 None:intergenic
AATCTCATCGGTGGTGGCGT+TGG 0.416328 7.4:+44019192 None:intergenic
TTAGGTGGAGGCGTTGGTAA+CGG 0.418036 7.4:-44019057 MS.gene90350:CDS
GGAGACTTAAATTTCCATAT+CGG 0.426564 7.4:-44019087 MS.gene90350:CDS
TCCAGCCAACTGGCATCAAC+AGG 0.444071 7.4:-44019807 MS.gene90350:intron
CTACTACAAATTCCAACTAT+AGG 0.452160 7.4:-44018639 MS.gene90350:CDS
AAGGGAAATTGATGAGTTTG+TGG 0.457842 7.4:+44018970 None:intergenic
CCGCTACCACCACCTTCAAA+TGG 0.459662 7.4:+44018904 None:intergenic
AGTGAAATAGGACTAAACTA+TGG 0.460875 7.4:-44019135 MS.gene90350:CDS
GCCCTATCTGCCATCGAACC+TGG 0.462544 7.4:+44019507 None:intergenic
TCCAGTCCAATGGGAGGTGT+TGG 0.464011 7.4:-44019108 MS.gene90350:CDS
GCCACCACCGATGAGATTCA+TGG 0.464462 7.4:-44019187 MS.gene90350:CDS
AATCAATCTAGACAATTCTT+GGG 0.469852 7.4:-44018799 MS.gene90350:CDS
GACCAGGTTCGATGGCAGAT+AGG 0.475722 7.4:-44019509 MS.gene90350:CDS
CTTAGAAGTGTCCCGGTTGG+CGG 0.481122 7.4:-44019330 MS.gene90350:CDS
CATATATGGATCCAGCCAAC+TGG 0.482920 7.4:-44019817 MS.gene90350:CDS
ATGGCTCCTTTGCATCATCT+AGG 0.483090 7.4:-44019168 MS.gene90350:CDS
ATAAAGTTATGTCCTATAGT+TGG 0.484745 7.4:+44018627 None:intergenic
ACCAGGTTCGATGGCAGATA+GGG 0.488883 7.4:-44019508 MS.gene90350:CDS
GAAGGTGGTGGTAGCGGTGG+TGG 0.489455 7.4:-44018898 MS.gene90350:CDS
GTCAGAGCTAGCCGGCGACT+TGG 0.489671 7.4:+44019260 None:intergenic
AGGTTCGATGGTTGAGGCTG+TGG 0.490154 7.4:+44019564 None:intergenic
TTCGACAAGCCTTGCAGAAG+TGG 0.490902 7.4:+44019376 None:intergenic
CATTGCTCAAAATTGCCAAC+AGG 0.493075 7.4:+44019000 None:intergenic
AGAAGTGTCCCGGTTGGCGG+TGG 0.494695 7.4:-44019327 MS.gene90350:CDS
CCATTTGAAGGTGGTGGTAG+CGG 0.496097 7.4:-44018904 MS.gene90350:CDS
AGAGCTAGCCGGCGACTTGG+AGG 0.502693 7.4:+44019263 None:intergenic
AGGTGGAGGCGTTGGTAACG+GGG 0.510318 7.4:-44019055 MS.gene90350:CDS
AGTTTAGTCCTATTTCACTT+AGG 0.513240 7.4:+44019139 None:intergenic
GATGGTTGAGGCTGTGGTGG+TGG 0.518267 7.4:+44019570 None:intergenic
GGATTGTTGTATCCATTTGA+AGG 0.522126 7.4:-44018916 MS.gene90350:CDS
TGGAATGCTTCTGCTGGTGG+TGG 0.522977 7.4:-44018715 MS.gene90350:CDS
TCCAACACCTCCCATTGGAC+TGG 0.527498 7.4:+44019107 None:intergenic
TGCGATGGTGTCAGGTTCGA+TGG 0.530120 7.4:+44019552 None:intergenic
AGCTTCTAAAGTTGACAAGA+AGG 0.532028 7.4:+44018951 None:intergenic
GTAGCAAAACTTTGTGTTGG+TGG 0.539364 7.4:+44019428 None:intergenic
GTAGTAGCAAAACTTTGTGT+TGG 0.542073 7.4:+44019425 None:intergenic
TTCGGCAACCACCGCCAACC+GGG 0.543263 7.4:+44019319 None:intergenic
AATGATCAATCCCATGCATA+TGG 0.543891 7.4:-44018871 MS.gene90350:CDS
AGCTCTTTAGGTGGAGGCGT+TGG 0.544707 7.4:-44019063 MS.gene90350:CDS
CTGACACCATCGCAACCTCA+TGG 0.545229 7.4:-44019543 MS.gene90350:CDS
CTCGATCAGACCAGGTTCGA+TGG 0.546965 7.4:-44019517 MS.gene90350:CDS
GCCATGAATCTCATCGGTGG+TGG 0.547604 7.4:+44019186 None:intergenic
CATGGAGGCTCGATCAGACC+AGG 0.549550 7.4:-44019525 MS.gene90350:CDS
TTGTATCCATTTGAAGGTGG+TGG 0.552400 7.4:-44018910 MS.gene90350:CDS
TGTCGAAGGTATTGGACAAA+GGG 0.552845 7.4:-44019360 MS.gene90350:CDS
GCAGCCACGCCACTTCTGCA+AGG 0.554356 7.4:-44019385 MS.gene90350:CDS
GCCCTTAGAAGTGTCCCGGT+TGG 0.556141 7.4:-44019333 MS.gene90350:CDS
ATCGGAAGCTCTTTAGGTGG+AGG 0.559518 7.4:-44019069 MS.gene90350:CDS
ATTTACCTGTTGATGCCAGT+TGG 0.559637 7.4:+44019802 None:intergenic
CAATATTGGAATGCTTCTGC+TGG 0.566637 7.4:-44018721 MS.gene90350:CDS
TGGTGCCCTTAGAAGTGTCC+CGG 0.567606 7.4:-44019337 MS.gene90350:CDS
TTCGATGGTTGAGGCTGTGG+TGG 0.570012 7.4:+44019567 None:intergenic
GTTGGGGAACTGAGTACCGC+AGG 0.570283 7.4:+44019210 None:intergenic
CATATCGGAAGCTCTTTAGG+TGG 0.571600 7.4:-44019072 MS.gene90350:CDS
AAGTCTCCAACACCTCCCAT+TGG 0.574092 7.4:+44019102 None:intergenic
TGTGGTGGTGGAATAAGTAG+AGG 0.574363 7.4:+44019582 None:intergenic
GATGGCAGATAGGGCAAGGA+TGG 0.575021 7.4:-44019499 MS.gene90350:CDS
TAGACACACCACCATATGCA+TGG 0.575174 7.4:+44018860 None:intergenic
TCTCATCGGTGGTGGCGTTG+GGG 0.576658 7.4:+44019194 None:intergenic
GCCAACCGGGACACTTCTAA+GGG 0.580076 7.4:+44019332 None:intergenic
GTTTGGCGGTCAGAGCTAGC+CGG 0.580677 7.4:+44019252 None:intergenic
TTAGAAGCTTCTTCTTCACA+TGG 0.581519 7.4:-44018937 MS.gene90350:CDS
TATTGGAATGCTTCTGCTGG+TGG 0.586587 7.4:-44018718 MS.gene90350:CDS
GTGGTTGCCGAAGAAACAAA+AGG 0.588845 7.4:-44019308 MS.gene90350:CDS
GCTTCTAAAGTTGACAAGAA+GGG 0.589264 7.4:+44018952 None:intergenic
TGTTTGTAGAGCTAGTTTGG+CGG 0.589509 7.4:+44019238 None:intergenic
TAACAACACCTCCAAGTCGC+CGG 0.589920 7.4:-44019271 MS.gene90350:CDS
CAAGCCTTGCAGAAGTGGCG+TGG 0.593138 7.4:+44019381 None:intergenic
GCAGCAACAAAATCATCAAG+TGG 0.596192 7.4:-44019625 MS.gene90350:intron
TTGTCGAAGGTATTGGACAA+AGG 0.596657 7.4:-44019361 MS.gene90350:CDS
GGTGGAGGCGTTGGTAACGG+GGG 0.601984 7.4:-44019054 MS.gene90350:CDS
TTTGAAGGTGGTGGTAGCGG+TGG 0.603652 7.4:-44018901 MS.gene90350:CDS
GAGCCTCCATGAGGTTGCGA+TGG 0.604696 7.4:+44019537 None:intergenic
AGGAGTGTTGACACTAGTGT+TGG 0.605835 7.4:+44019602 None:intergenic
GGTGTCAGGTTCGATGGTTG+AGG 0.608734 7.4:+44019558 None:intergenic
TTGGCTGGATCCATATATGG+TGG 0.609224 7.4:+44019821 None:intergenic
GTTCGATGGCAGATAGGGCA+AGG 0.614474 7.4:-44019503 MS.gene90350:CDS
AGACACACCACCATATGCAT+GGG 0.619832 7.4:+44018861 None:intergenic
TTGTTGTATCCATTTGAAGG+TGG 0.620036 7.4:-44018913 MS.gene90350:CDS
TGATCACCTAGATGATGCAA+AGG 0.622493 7.4:+44019162 None:intergenic
CGAAGGTATTGGACAAAGGG+TGG 0.625409 7.4:-44019357 MS.gene90350:CDS
TGGCTGGATCCATATATGGT+GGG 0.628650 7.4:+44019822 None:intergenic
AAAGGAGCCATGAATCTCAT+CGG 0.638198 7.4:+44019180 None:intergenic
ACTTCTGCAAGGCTTGTCGA+AGG 0.638852 7.4:-44019374 MS.gene90350:CDS
GCTGTAGAAAGATCTGTCCA+AGG 0.641214 7.4:+44018679 None:intergenic
GTGGTGCTACTAATTCACCT+TGG 0.642878 7.4:-44018696 MS.gene90350:CDS
CTCTACAAACAACTCTCCTG+CGG 0.682618 7.4:-44019226 MS.gene90350:CDS
GTGTTGGTGGAATCACACCT+TGG 0.687306 7.4:+44019441 None:intergenic
GGTCTGATCGAGCCTCCATG+AGG 0.689453 7.4:+44019528 None:intergenic
GGAGCCATGAATCTCATCGG+TGG 0.692277 7.4:+44019183 None:intergenic
GATCAATCCCATGCATATGG+TGG 0.692633 7.4:-44018868 MS.gene90350:CDS
TGGGAGAGAAGTATAAACCA+TGG 0.714065 7.4:+44019841 None:intergenic
ACACCATCGCAACCTCATGG+AGG 0.720835 7.4:-44019540 MS.gene90350:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAGGAAATAAAAATTAA+TGG + chr7.4:44018826-44018845 None:intergenic 10.0%
!! GATACATAAAATCAAATATT+TGG + chr7.4:44018799-44018818 None:intergenic 15.0%
!! AAATAATTCTAGTGTGAAAA+TGG - chr7.4:44019645-44019664 MS.gene90350:intron 20.0%
! AATCAATCTAGACAATTCTT+GGG - chr7.4:44019673-44019692 MS.gene90350:intron 25.0%
! ATCCTAATAGTGAACAATAT+TGG - chr7.4:44019737-44019756 MS.gene90350:intron 25.0%
! ATTTGGTATATTCAAGAGAA+GGG + chr7.4:44018782-44018801 None:intergenic 25.0%
! TATTTGGTATATTCAAGAGA+AGG + chr7.4:44018783-44018802 None:intergenic 25.0%
! TTCCAATATTGTTCACTATT+AGG + chr7.4:44019742-44019761 None:intergenic 25.0%
!!! ATTTAGACCTTTTGTTTCTT+CGG + chr7.4:44019174-44019193 None:intergenic 25.0%
AGTGAAATAGGACTAAACTA+TGG - chr7.4:44019337-44019356 MS.gene90350:CDS 30.0%
AGTTTAGTCCTATTTCACTT+AGG + chr7.4:44019336-44019355 None:intergenic 30.0%
ATCAACAGGTAAATCAACAA+GGG - chr7.4:44018679-44018698 MS.gene90350:CDS 30.0%
CTACTACAAATTCCAACTAT+AGG - chr7.4:44019833-44019852 MS.gene90350:CDS 30.0%
GAATCAATCTAGACAATTCT+TGG - chr7.4:44019672-44019691 MS.gene90350:intron 30.0%
GGAGACTTAAATTTCCATAT+CGG - chr7.4:44019385-44019404 MS.gene90350:CDS 30.0%
! TGATTTTGTTGCTGCAAAAA+AGG + chr7.4:44018842-44018861 None:intergenic 30.0%
AACCATCACAAAAATGTCCA+AGG - chr7.4:44019014-44019033 MS.gene90350:CDS 35.0%
AAGGGAAATTGATGAGTTTG+TGG + chr7.4:44019505-44019524 None:intergenic 35.0%
AATGATCAATCCCATGCATA+TGG - chr7.4:44019601-44019620 MS.gene90350:CDS 35.0%
AGTTGTTTGTAGAGCTAGTT+TGG + chr7.4:44019240-44019259 None:intergenic 35.0%
CATCAACAGGTAAATCAACA+AGG - chr7.4:44018678-44018697 MS.gene90350:CDS 35.0%
GGATTGTTGTATCCATTTGA+AGG - chr7.4:44019556-44019575 MS.gene90350:CDS 35.0%
GTAGTAGCAAAACTTTGTGT+TGG + chr7.4:44019050-44019069 None:intergenic 35.0%
TCAACAAGGGTTCTTCAATT+AGG - chr7.4:44018692-44018711 MS.gene90350:CDS 35.0%
TTAGAAGCTTCTTCTTCACA+TGG - chr7.4:44019535-44019554 MS.gene90350:CDS 35.0%
TTGTTGTATCCATTTGAAGG+TGG - chr7.4:44019559-44019578 MS.gene90350:CDS 35.0%
! TATGGTTTTTCCAGTCCAAT+GGG - chr7.4:44019355-44019374 MS.gene90350:CDS 35.0%
! TCTACTTCGATTTTACCTGT+TGG - chr7.4:44019457-44019476 MS.gene90350:CDS 35.0%
! TTTTGTGATGGTTCTTGCAT+AGG + chr7.4:44019007-44019026 None:intergenic 35.0%
!! AGCTTCTAAAGTTGACAAGA+AGG + chr7.4:44019524-44019543 None:intergenic 35.0%
!! GCTTCTAAAGTTGACAAGAA+GGG + chr7.4:44019523-44019542 None:intergenic 35.0%
AAAGGAGCCATGAATCTCAT+CGG + chr7.4:44019295-44019314 None:intergenic 40.0%
ATTTACCTGTTGATGCCAGT+TGG + chr7.4:44018673-44018692 None:intergenic 40.0%
CAATATTGGAATGCTTCTGC+TGG - chr7.4:44019751-44019770 MS.gene90350:intron 40.0%
CATTGCTCAAAATTGCCAAC+AGG + chr7.4:44019475-44019494 None:intergenic 40.0%
GCAGCAACAAAATCATCAAG+TGG - chr7.4:44018847-44018866 MS.gene90350:CDS 40.0%
GGTGATCACCTAAGTGAAAT+AGG - chr7.4:44019325-44019344 MS.gene90350:CDS 40.0%
GTAGCAAAACTTTGTGTTGG+TGG + chr7.4:44019047-44019066 None:intergenic 40.0%
TACTTCTCTCCCACCATATA+TGG - chr7.4:44018641-44018660 MS.gene90350:CDS 40.0%
TGATCACCTAGATGATGCAA+AGG + chr7.4:44019313-44019332 None:intergenic 40.0%
TTCCATATCGGAAGCTCTTT+AGG - chr7.4:44019397-44019416 MS.gene90350:CDS 40.0%
TTGTATCCATTTGAAGGTGG+TGG - chr7.4:44019562-44019581 MS.gene90350:CDS 40.0%
! CACCTTGGACATTTTTGTGA+TGG + chr7.4:44019019-44019038 None:intergenic 40.0%
! CTATGGTTTTTCCAGTCCAA+TGG - chr7.4:44019354-44019373 MS.gene90350:CDS 40.0%
! CTGTTGGCAATTTTGAGCAA+TGG - chr7.4:44019473-44019492 MS.gene90350:CDS 40.0%
! TGTTTGTAGAGCTAGTTTGG+CGG + chr7.4:44019237-44019256 None:intergenic 40.0%
! TTGGCAATTTTGAGCAATGG+AGG - chr7.4:44019476-44019495 MS.gene90350:CDS 40.0%
!! TGTCGAAGGTATTGGACAAA+GGG - chr7.4:44019112-44019131 MS.gene90350:CDS 40.0%
!! TTGTCGAAGGTATTGGACAA+AGG - chr7.4:44019111-44019130 MS.gene90350:CDS 40.0%
AGACACACCACCATATGCAT+GGG + chr7.4:44019614-44019633 None:intergenic 45.0%
ATGGCTCCTTTGCATCATCT+AGG - chr7.4:44019304-44019323 MS.gene90350:CDS 45.0%
CACCTAAAGAGCTTCCGATA+TGG + chr7.4:44019402-44019421 None:intergenic 45.0%
CAGTTGGCTGGATCCATATA+TGG + chr7.4:44018657-44018676 None:intergenic 45.0%
CATATATGGATCCAGCCAAC+TGG - chr7.4:44018655-44018674 MS.gene90350:CDS 45.0%
CATATCGGAAGCTCTTTAGG+TGG - chr7.4:44019400-44019419 MS.gene90350:CDS 45.0%
CTCTACAAACAACTCTCCTG+CGG - chr7.4:44019246-44019265 MS.gene90350:CDS 45.0%
GATCAATCCCATGCATATGG+TGG - chr7.4:44019604-44019623 MS.gene90350:CDS 45.0%
GCTGTAGAAAGATCTGTCCA+AGG + chr7.4:44019796-44019815 None:intergenic 45.0%
GTGGTTGCCGAAGAAACAAA+AGG - chr7.4:44019164-44019183 MS.gene90350:CDS 45.0%
TAGACACACCACCATATGCA+TGG + chr7.4:44019615-44019634 None:intergenic 45.0%
TATTGGAATGCTTCTGCTGG+TGG - chr7.4:44019754-44019773 MS.gene90350:intron 45.0%
TGGCTGGATCCATATATGGT+GGG + chr7.4:44018653-44018672 None:intergenic 45.0%
TTGGCTGGATCCATATATGG+TGG + chr7.4:44018654-44018673 None:intergenic 45.0%
! TGCATATGGTGGTGTGTCTA+AGG - chr7.4:44019615-44019634 MS.gene90350:CDS 45.0%
!! AGGAGTGTTGACACTAGTGT+TGG + chr7.4:44018873-44018892 None:intergenic 45.0%
!! GTGGTGCTACTAATTCACCT+TGG - chr7.4:44019776-44019795 MS.gene90350:intron 45.0%
!! TGTGGTGGTGGAATAAGTAG+AGG + chr7.4:44018893-44018912 None:intergenic 45.0%
AAGTCTCCAACACCTCCCAT+TGG + chr7.4:44019373-44019392 None:intergenic 50.0%
ACCAGGTTCGATGGCAGATA+GGG - chr7.4:44018964-44018983 MS.gene90350:CDS 50.0%
ACTTCTGCAAGGCTTGTCGA+AGG - chr7.4:44019098-44019117 MS.gene90350:CDS 50.0%
ATCGGAAGCTCTTTAGGTGG+AGG - chr7.4:44019403-44019422 MS.gene90350:CDS 50.0%
TAACAACACCTCCAAGTCGC+CGG - chr7.4:44019201-44019220 MS.gene90350:CDS 50.0%
TTCGACAAGCCTTGCAGAAG+TGG + chr7.4:44019099-44019118 None:intergenic 50.0%
! CCATTTGAAGGTGGTGGTAG+CGG - chr7.4:44019568-44019587 MS.gene90350:CDS 50.0%
! GCAAGGCTTGTCGAAGGTAT+TGG - chr7.4:44019104-44019123 MS.gene90350:CDS 50.0%
! GGTTTTTCCAGTCCAATGGG+AGG - chr7.4:44019358-44019377 MS.gene90350:CDS 50.0%
! GTGTTGGTGGAATCACACCT+TGG + chr7.4:44019034-44019053 None:intergenic 50.0%
!! CGAAGGTATTGGACAAAGGG+TGG - chr7.4:44019115-44019134 MS.gene90350:CDS 50.0%
!! TTAGGTGGAGGCGTTGGTAA+CGG - chr7.4:44019415-44019434 MS.gene90350:CDS 50.0%
ACACCATCGCAACCTCATGG+AGG - chr7.4:44018932-44018951 MS.gene90350:CDS 55.0%
ACCTGTTGATGCCAGTTGGC+TGG + chr7.4:44018669-44018688 None:intergenic 55.0%
AGGTTCGATGGTTGAGGCTG+TGG + chr7.4:44018911-44018930 None:intergenic 55.0%
CATGAGGTTGCGATGGTGTC+AGG + chr7.4:44018931-44018950 None:intergenic 55.0%
CCGCTACCACCACCTTCAAA+TGG + chr7.4:44019571-44019590 None:intergenic 55.0%
CTCGATCAGACCAGGTTCGA+TGG - chr7.4:44018955-44018974 MS.gene90350:CDS 55.0%
CTGACACCATCGCAACCTCA+TGG - chr7.4:44018929-44018948 MS.gene90350:CDS 55.0%
GACCAGGTTCGATGGCAGAT+AGG - chr7.4:44018963-44018982 MS.gene90350:CDS 55.0%
GATGGCAGATAGGGCAAGGA+TGG - chr7.4:44018973-44018992 MS.gene90350:CDS 55.0%
GCCAACCGGGACACTTCTAA+GGG + chr7.4:44019143-44019162 None:intergenic 55.0%
GCCACCACCGATGAGATTCA+TGG - chr7.4:44019285-44019304 MS.gene90350:CDS 55.0%
GCCATGAATCTCATCGGTGG+TGG + chr7.4:44019289-44019308 None:intergenic 55.0%
GGAGCCATGAATCTCATCGG+TGG + chr7.4:44019292-44019311 None:intergenic 55.0%
GGTGTCAGGTTCGATGGTTG+AGG + chr7.4:44018917-44018936 None:intergenic 55.0%
GTTCGATGGCAGATAGGGCA+AGG - chr7.4:44018969-44018988 MS.gene90350:CDS 55.0%
TCCAACACCTCCCATTGGAC+TGG + chr7.4:44019368-44019387 None:intergenic 55.0%
TCCAGCCAACTGGCATCAAC+AGG - chr7.4:44018665-44018684 MS.gene90350:CDS 55.0%
TGGAATGCTTCTGCTGGTGG+TGG - chr7.4:44019757-44019776 MS.gene90350:intron 55.0%
TTCGATGGTTGAGGCTGTGG+TGG + chr7.4:44018908-44018927 None:intergenic 55.0%
! AATCTCATCGGTGGTGGCGT+TGG + chr7.4:44019283-44019302 None:intergenic 55.0%
! AGCTCTTTAGGTGGAGGCGT+TGG - chr7.4:44019409-44019428 MS.gene90350:CDS 55.0%
! ATCTCATCGGTGGTGGCGTT+GGG + chr7.4:44019282-44019301 None:intergenic 55.0%
! TCCAGTCCAATGGGAGGTGT+TGG - chr7.4:44019364-44019383 MS.gene90350:CDS 55.0%
! TGCGATGGTGTCAGGTTCGA+TGG + chr7.4:44018923-44018942 None:intergenic 55.0%
!! CTTAGAAGTGTCCCGGTTGG+CGG - chr7.4:44019142-44019161 MS.gene90350:CDS 55.0%
!! GTTGGTAACGGGGGTGTCAA+CGG - chr7.4:44019427-44019446 MS.gene90350:CDS 55.0%
!! TAGGTGGAGGCGTTGGTAAC+GGG - chr7.4:44019416-44019435 MS.gene90350:CDS 55.0%
!! TGGTGCCCTTAGAAGTGTCC+CGG - chr7.4:44019135-44019154 MS.gene90350:CDS 55.0%
!! TTTGAAGGTGGTGGTAGCGG+TGG - chr7.4:44019571-44019590 MS.gene90350:CDS 55.0%
CAAGCCTTGCAGAAGTGGCG+TGG + chr7.4:44019094-44019113 None:intergenic 60.0%
CATGGAGGCTCGATCAGACC+AGG - chr7.4:44018947-44018966 MS.gene90350:CDS 60.0%
CGCCAACCGGGACACTTCTA+AGG + chr7.4:44019144-44019163 None:intergenic 60.0%
GAGCCTCCATGAGGTTGCGA+TGG + chr7.4:44018938-44018957 None:intergenic 60.0%
GATGGTTGAGGCTGTGGTGG+TGG + chr7.4:44018905-44018924 None:intergenic 60.0%
GCCCTATCTGCCATCGAACC+TGG + chr7.4:44018968-44018987 None:intergenic 60.0%
GGTCTGATCGAGCCTCCATG+AGG + chr7.4:44018947-44018966 None:intergenic 60.0%
GTTTGGCGGTCAGAGCTAGC+CGG + chr7.4:44019223-44019242 None:intergenic 60.0%
! GTTGGGGAACTGAGTACCGC+AGG + chr7.4:44019265-44019284 None:intergenic 60.0%
! TCTCATCGGTGGTGGCGTTG+GGG + chr7.4:44019281-44019300 None:intergenic 60.0%
!! AGGTGGAGGCGTTGGTAACG+GGG - chr7.4:44019417-44019436 MS.gene90350:CDS 60.0%
!! GCCCTTAGAAGTGTCCCGGT+TGG - chr7.4:44019139-44019158 MS.gene90350:CDS 60.0%
CTTCGGCAACCACCGCCAAC+CGG + chr7.4:44019157-44019176 None:intergenic 65.0%
GCAGCCACGCCACTTCTGCA+AGG - chr7.4:44019087-44019106 MS.gene90350:CDS 65.0%
GTCAGAGCTAGCCGGCGACT+TGG + chr7.4:44019215-44019234 None:intergenic 65.0%
TTCGGCAACCACCGCCAACC+GGG + chr7.4:44019156-44019175 None:intergenic 65.0%
! AGAGCTAGCCGGCGACTTGG+AGG + chr7.4:44019212-44019231 None:intergenic 65.0%
!! AACGGGGGTGTCAACGGTGG+TGG - chr7.4:44019433-44019452 MS.gene90350:CDS 65.0%
!! AGAAGTGTCCCGGTTGGCGG+TGG - chr7.4:44019145-44019164 MS.gene90350:CDS 65.0%
!! GAAGGTGGTGGTAGCGGTGG+TGG - chr7.4:44019574-44019593 MS.gene90350:CDS 65.0%
!! GGTAACGGGGGTGTCAACGG+TGG - chr7.4:44019430-44019449 MS.gene90350:CDS 65.0%
!! GGTGGAGGCGTTGGTAACGG+GGG - chr7.4:44019418-44019437 MS.gene90350:CDS 65.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 44018633 44019861 44018633 ID=MS.gene90350
chr7.4 mRNA 44018633 44019861 44018633 ID=MS.gene90350.t1;Parent=MS.gene90350
chr7.4 exon 44019808 44019861 44019808 ID=MS.gene90350.t1.exon1;Parent=MS.gene90350.t1
chr7.4 CDS 44019808 44019861 44019808 ID=cds.MS.gene90350.t1;Parent=MS.gene90350.t1
chr7.4 exon 44018633 44019643 44018633 ID=MS.gene90350.t1.exon2;Parent=MS.gene90350.t1
chr7.4 CDS 44018633 44019643 44018633 ID=cds.MS.gene90350.t1;Parent=MS.gene90350.t1
Gene Sequence

>MS.gene90350

ATGGTTTATACTTCTCTCCCACCATATATGGATCCAGCCAACTGGCATCAACAGGTAAATCAACAAGGGTTCTTCAATTAGGTTTCAAAAAGAAAGAAAAAAAATAAACTCAAAAGATAAATATTCGAGCTTTACTTTTGTTTATACCCTTCTCTTGAATATACCAAATATTTGATTTTATGTATCTTTACCATTAATTTTTATTTCCTTTTTTGCAGCAACAAAATCATCAAGTGGCCAACACTAGTGTCAACACTCCTCTACTTATTCCACCACCACAGCCTCAACCATCGAACCTGACACCATCGCAACCTCATGGAGGCTCGATCAGACCAGGTTCGATGGCAGATAGGGCAAGGATGGCAAACATACCTATGCAAGAACCATCACAAAAATGTCCAAGGTGTGATTCCACCAACACAAAGTTTTGCTACTACAACAACTATAGTCTCTCGCAGCCACGCCACTTCTGCAAGGCTTGTCGAAGGTATTGGACAAAGGGTGGTGCCCTTAGAAGTGTCCCGGTTGGCGGTGGTTGCCGAAGAAACAAAAGGTCTAAATCAAGCAGTAACAACACCTCCAAGTCGCCGGCTAGCTCTGACCGCCAAACTAGCTCTACAAACAACTCTCCTGCGGTACTCAGTTCCCCAACGCCACCACCGATGAGATTCATGGCTCCTTTGCATCATCTAGGTGATCACCTAAGTGAAATAGGACTAAACTATGGTTTTTCCAGTCCAATGGGAGGTGTTGGAGACTTAAATTTCCATATCGGAAGCTCTTTAGGTGGAGGCGTTGGTAACGGGGGTGTCAACGGTGGTGGTTCTACTTCGATTTTACCTGTTGGCAATTTTGAGCAATGGAGGATGCCACAAACTCATCAATTTCCCTTCTTGTCAACTTTAGAAGCTTCTTCTTCACATGGATTGTTGTATCCATTTGAAGGTGGTGGTAGCGGTGGTGGTGCTAATGATCAATCCCATGCATATGGTGGTGTGTCTAAGGTATTATCAAATAATTCTAGTGTGAAAATGGAAGAGAATCAATCTAGACAATTCTTGGGAATGATCAACAAAAACAACATCAATAATAATAATAATAATAATCCTAATAGTGAACAATATTGGAATGCTTCTGCTGGTGGTGGTGCTACTAATTCACCTTGGACAGATCTTTCTACAGCTTATAACACTTCTTCAGCTACTACAAATTCCAACTATAGGACATAA
Protein sequence

>MS.gene90350.t1

MVYTSLPPYMDPANWHQQQQNHQVANTSVNTPLLIPPPQPQPSNLTPSQPHGGSIRPGSMADRARMANIPMQEPSQKCPRCDSTNTKFCYYNNYSLSQPRHFCKACRRYWTKGGALRSVPVGGGCRRNKRSKSSSNNTSKSPASSDRQTSSTNNSPAVLSSPTPPPMRFMAPLHHLGDHLSEIGLNYGFSSPMGGVGDLNFHIGSSLGGGVGNGGVNGGGSTSILPVGNFEQWRMPQTHQFPFLSTLEASSSHGLLYPFEGGGSGGGANDQSHAYGGVSKVLSNNSSVKMEENQSRQFLGMINKNNINNNNNNNPNSEQYWNASAGGGATNSPWTDLSTAYNTSSATTNSNYRT