AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene90735


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene90735.t1 MTR_1g023230 99.174 121 0 1 1 120 108 228 6.02e-83 248
MS.gene90735.t1 MTR_3g112510 59.910 222 69 7 11 225 82 290 7.56e-73 223
MS.gene90735.t1 MTR_8g076620 54.357 241 78 7 1 228 110 331 2.45e-68 213
MS.gene90735.t1 MTR_5g021130 54.661 236 89 6 7 228 92 323 4.15e-68 212
MS.gene90735.t1 MTR_5g089750 45.062 162 81 3 15 168 249 410 1.21e-38 139
MS.gene90735.t1 MTR_3g014660 40.113 177 95 4 1 167 119 294 2.14e-35 128
MS.gene90735.t1 MTR_4g071110 41.892 148 62 2 12 159 14 137 5.98e-33 120
MS.gene90735.t1 MTR_8g039110 39.779 181 95 3 15 181 124 304 4.80e-32 119
MS.gene90735.t1 MTR_0363s0040 75.556 45 11 0 15 59 124 168 2.38e-20 86.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene90735.t1 AT1G75520 52.564 234 87 6 11 228 121 346 7.05e-73 225
MS.gene90735.t1 AT3G51060 50.424 236 90 4 13 228 142 370 5.30e-70 219
MS.gene90735.t1 AT1G19790 50.410 244 103 7 1 227 102 344 5.81e-69 215
MS.gene90735.t1 AT1G19790 50.410 244 103 7 1 227 102 344 5.81e-69 215
MS.gene90735.t1 AT5G66350 49.554 224 91 6 15 228 120 331 2.26e-63 200
MS.gene90735.t1 AT5G66350 48.402 219 91 6 15 223 120 326 1.05e-60 193
MS.gene90735.t1 AT5G66350 49.296 213 86 6 15 217 120 320 2.18e-59 190
MS.gene90735.t1 AT2G21400 42.991 214 83 6 12 222 7 184 1.96e-48 157
MS.gene90735.t1 AT2G21400 42.991 214 83 6 12 222 6 183 2.29e-48 157
MS.gene90735.t1 AT2G21400 42.180 211 74 6 12 222 7 169 2.20e-46 152
MS.gene90735.t1 AT2G21400 42.180 211 74 6 12 222 6 168 2.54e-46 152
MS.gene90735.t1 AT4G36260 52.500 160 60 5 13 161 92 246 4.23e-46 155
MS.gene90735.t1 AT2G18120 47.436 156 56 3 13 161 70 206 1.60e-41 141
MS.gene90735.t1 AT2G18120 48.344 151 60 2 13 161 70 204 5.03e-39 134
MS.gene90735.t1 AT5G12330 47.500 160 71 2 15 161 112 271 1.05e-36 131
MS.gene90735.t1 AT5G12330 47.500 160 71 2 15 161 112 271 1.05e-36 131
MS.gene90735.t1 AT5G12330 47.500 160 71 2 15 161 199 358 1.55e-36 132
MS.gene90735.t1 AT5G33210 61.538 91 32 1 13 103 15 102 8.41e-33 115
MS.gene90735.t1 AT5G33210 49.206 126 54 3 13 138 50 165 1.40e-32 116
MS.gene90735.t1 AT3G54430 34.783 161 82 4 11 167 37 178 9.14e-24 94.4
MS.gene90735.t1 AT5G12330 82.609 46 8 0 15 60 112 157 9.40e-22 90.1

Find 59 sgRNAs with CRISPR-Local

Find 74 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATGATCCCTCTGGAGCTTC+TGG 0.278538 1.4:+1450155 MS.gene90735:CDS
AGCTCCTCTTAATGCTTTCA+TGG 0.295162 1.4:+1451017 MS.gene90735:CDS
CACTCATGTCTTTAGGATCT+TGG 0.300540 1.4:-1451063 None:intergenic
TGAATTCACCGGCGGTGTTC+AGG 0.312818 1.4:+1450737 MS.gene90735:CDS
TATATACCTGTGGTAGTAAT+AGG 0.332609 1.4:-1450200 None:intergenic
AGGTCTTCAAGTGGTGGTTT+AGG 0.350386 1.4:+1449900 MS.gene90735:CDS
GGTGGTGGCGAGGGTTCTTC+CGG 0.367254 1.4:+1450901 MS.gene90735:CDS
CCACCACCAGCAGCAGCATT+TGG 0.392576 1.4:-1450886 None:intergenic
CGTTCGCGGCGTTTAGCAGC+TGG 0.397352 1.4:-1450050 None:intergenic
CCTCTTAATGCTTTCATGGC+TGG 0.408461 1.4:+1451021 MS.gene90735:CDS
GTGGACAATCTTTCTTAGCT+TGG 0.415642 1.4:-1449956 None:intergenic
TGAGTTGTTGCTGCCGTTCG+CGG 0.429437 1.4:-1450064 None:intergenic
GGCGAGGGTTCTTCCGGTGG+TGG 0.430350 1.4:+1450907 MS.gene90735:CDS
TTGAAATTAGGGTTGGAGTT+GGG 0.437119 1.4:+1450697 MS.gene90735:intron
AGTGGTGGTTTAGGTGGTGG+TGG 0.442865 1.4:+1449909 MS.gene90735:CDS
GGTTCTTCCGGTGGTGGCAG+CGG 0.446891 1.4:+1450913 MS.gene90735:CDS
TTCGCCACCGCTGCCACCAC+CGG 0.449406 1.4:-1450920 None:intergenic
CCAAATGCTGCTGCTGGTGG+TGG 0.457278 1.4:+1450886 MS.gene90735:CDS
AAACTCATGTGAAAAGTACT+TGG 0.464467 1.4:+1450024 MS.gene90735:CDS
TGCTGCTGCTGGTGGTGGCG+AGG 0.465038 1.4:+1450891 MS.gene90735:CDS
TTGTATGATCAAGGACTTGA+AGG 0.474373 1.4:+1450850 MS.gene90735:CDS
GAAAATCAAAATGATCCCTC+TGG 0.474468 1.4:+1450146 MS.gene90735:CDS
TGTCTTTAGGATCTTGGAGG+TGG 0.488834 1.4:-1451057 None:intergenic
GCTGCTGCTGGTGGTGGCGA+GGG 0.489260 1.4:+1450892 MS.gene90735:CDS
TCATGTCTTTAGGATCTTGG+AGG 0.489282 1.4:-1451060 None:intergenic
TATAGTGAAGAATACTCAAA+TGG 0.492700 1.4:-1450991 None:intergenic
TCAAGTGGTGGTTTAGGTGG+TGG 0.495997 1.4:+1449906 MS.gene90735:CDS
ATGAAAGCATTAAGAGGAGC+TGG 0.499046 1.4:-1451015 None:intergenic
CCTGTGGTAGTAATAGGTAC+TGG 0.504192 1.4:-1450194 None:intergenic
TCTTCCGGTGGTGGCAGCGG+TGG 0.515802 1.4:+1450916 MS.gene90735:CDS
TCTTCAAGTGGTGGTTTAGG+TGG 0.516785 1.4:+1449903 MS.gene90735:CDS
AGTAATAGGTACTGGAGTAC+AGG 0.518362 1.4:-1450186 None:intergenic
CCAGCCATGAAAGCATTAAG+AGG 0.523927 1.4:-1451021 None:intergenic
TTTGAAATTAGGGTTGGAGT+TGG 0.534737 1.4:+1450696 MS.gene90735:intron
GGTGGTGGCAGCGGTGGCGA+AGG 0.539736 1.4:+1450922 MS.gene90735:CDS
TTTACACACCTGAACACCGC+CGG 0.552531 1.4:-1450745 None:intergenic
TCCACCGGAGCTGAATTCAC+CGG 0.555263 1.4:+1450726 MS.gene90735:CDS
GAACTTGTTGCAAGAGTCGA+GGG 0.557726 1.4:+1449991 MS.gene90735:CDS
CAGCTGCTAAACGCCGCGAA+CGG 0.557914 1.4:+1450051 MS.gene90735:CDS
ATTGTTAACATTTGTTGTCG+TGG 0.557983 1.4:-1450968 None:intergenic
GTCTTTAGGATCTTGGAGGT+GGG 0.561171 1.4:-1451056 None:intergenic
CCAGTACCTATTACTACCAC+AGG 0.564967 1.4:+1450194 MS.gene90735:CDS
CAAACTGCTGTGAACATAGG+AGG 0.565014 1.4:+1450808 MS.gene90735:CDS
ACTAGTCCAAATGCTGCTGC+TGG 0.565581 1.4:+1450880 MS.gene90735:CDS
GCCGGTGAATTCAGCTCCGG+TGG 0.569608 1.4:-1450727 None:intergenic
TATCAAACTGCTGTGAACAT+AGG 0.572121 1.4:+1450805 MS.gene90735:CDS
GGTGGCGAGGGTTCTTCCGG+TGG 0.572206 1.4:+1450904 MS.gene90735:CDS
ACCGCCGGTGAATTCAGCTC+CGG 0.574562 1.4:-1450730 None:intergenic
TAGAGAACCAGAAGCTCCAG+AGG 0.578477 1.4:-1450162 None:intergenic
AGTCCAAATGCTGCTGCTGG+TGG 0.584415 1.4:+1450883 MS.gene90735:CDS
AACTCATGTGAAAAGTACTT+GGG 0.591492 1.4:+1450025 MS.gene90735:CDS
AGAACTTGTTGCAAGAGTCG+AGG 0.603647 1.4:+1449990 MS.gene90735:CDS
GTGATGAGGTCTTCAAGTGG+TGG 0.621009 1.4:+1449894 None:intergenic
CAAGTTCTACATCTAAGATG+TGG 0.632750 1.4:-1449975 None:intergenic
GGAATGAACTGTCAAGATTG+TGG 0.658447 1.4:+1449930 MS.gene90735:CDS
ACCGGAGCTGAATTCACCGG+CGG 0.668533 1.4:+1450729 MS.gene90735:CDS
AGAGAACCAGAAGCTCCAGA+GGG 0.671479 1.4:-1450161 None:intergenic
GTGTGTAAAAGTGAGTGCAA+TGG 0.678715 1.4:+1450759 MS.gene90735:CDS
ACAGTGATGAGGTCTTCAAG+TGG 0.705760 1.4:+1449891 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTATTTGATTTGAAATTA+GGG + chr1.4:1450686-1450705 MS.gene90735:intron 10.0%
!!! TTTTTATTTGATTTGAAATT+AGG + chr1.4:1450685-1450704 MS.gene90735:intron 10.0%
!! ATAGAATATATAGACTAATT+AGG - chr1.4:1450470-1450489 None:intergenic 15.0%
!!! TCTTTTTGAATTTTTTGATT+TGG + chr1.4:1450650-1450669 MS.gene90735:intron 15.0%
!! AAAATGAAAAGATATTACGA+GGG + chr1.4:1450317-1450336 MS.gene90735:intron 20.0%
!! TAAAATGAAAAGATATTACG+AGG + chr1.4:1450316-1450335 MS.gene90735:intron 20.0%
!! TCTATGAATCTATGATAAAT+AGG + chr1.4:1450441-1450460 MS.gene90735:intron 20.0%
!!! TAGTTTTTTGATATATAGCT+AGG + chr1.4:1450356-1450375 MS.gene90735:intron 20.0%
! TAGCTATATAGAAACAATTG+AGG + chr1.4:1450623-1450642 MS.gene90735:intron 25.0%
! TATAGTGAAGAATACTCAAA+TGG - chr1.4:1450994-1451013 None:intergenic 25.0%
! TGAAAAAATTGTTAGGTAAG+TGG + chr1.4:1450571-1450590 MS.gene90735:intron 25.0%
! TGAAGGATGAAAAAATTGTT+AGG + chr1.4:1450564-1450583 MS.gene90735:intron 25.0%
! TTTCTACATATATATACCTG+TGG - chr1.4:1450213-1450232 None:intergenic 25.0%
!!! ATTTGATTTGAAATTAGGGT+TGG + chr1.4:1450690-1450709 MS.gene90735:intron 25.0%
TATATACCTGTGGTAGTAAT+AGG - chr1.4:1450203-1450222 None:intergenic 30.0%
TTGATAGAGTATGAAAGGAA+TGG - chr1.4:1450497-1450516 None:intergenic 30.0%
! AAGGGAATTTTGTATGATCA+AGG + chr1.4:1450841-1450860 MS.gene90735:CDS 30.0%
! TTCTCTTTCTTTTCTTCTGA+AGG + chr1.4:1450547-1450566 MS.gene90735:intron 30.0%
!! AAACTCATGTGAAAAGTACT+TGG + chr1.4:1450024-1450043 MS.gene90735:CDS 30.0%
!! AACTCATGTGAAAAGTACTT+GGG + chr1.4:1450025-1450044 MS.gene90735:CDS 30.0%
!! ATTGTTAACATTTGTTGTCG+TGG - chr1.4:1450971-1450990 None:intergenic 30.0%
!! CTAAGTTGATAGAGTATGAA+AGG - chr1.4:1450502-1450521 None:intergenic 30.0%
GAAAATCAAAATGATCCCTC+TGG + chr1.4:1450146-1450165 MS.gene90735:CDS 35.0%
TATCAAACTGCTGTGAACAT+AGG + chr1.4:1450805-1450824 MS.gene90735:CDS 35.0%
TTGAAATTAGGGTTGGAGTT+GGG + chr1.4:1450697-1450716 MS.gene90735:intron 35.0%
TTGTATGATCAAGGACTTGA+AGG + chr1.4:1450850-1450869 MS.gene90735:CDS 35.0%
! ATAGGAGGAAGAGTTTTCAA+GGG + chr1.4:1450823-1450842 MS.gene90735:CDS 35.0%
! CAAGTTCTACATCTAAGATG+TGG - chr1.4:1449978-1449997 None:intergenic 35.0%
! TTTGAAATTAGGGTTGGAGT+TGG + chr1.4:1450696-1450715 MS.gene90735:intron 35.0%
AGCTCCTCTTAATGCTTTCA+TGG + chr1.4:1451017-1451036 MS.gene90735:CDS 40.0%
GGAATGAACTGTCAAGATTG+TGG + chr1.4:1449930-1449949 MS.gene90735:CDS 40.0%
GTGGACAATCTTTCTTAGCT+TGG - chr1.4:1449959-1449978 None:intergenic 40.0%
GTGTGTAAAAGTGAGTGCAA+TGG + chr1.4:1450759-1450778 MS.gene90735:CDS 40.0%
! CATAGGAGGAAGAGTTTTCA+AGG + chr1.4:1450822-1450841 MS.gene90735:CDS 40.0%
!! AGTAATAGGTACTGGAGTAC+AGG - chr1.4:1450189-1450208 None:intergenic 40.0%
!! ATGAAAGCATTAAGAGGAGC+TGG - chr1.4:1451018-1451037 None:intergenic 40.0%
AGGTCTTCAAGTGGTGGTTT+AGG + chr1.4:1449900-1449919 MS.gene90735:CDS 45.0%
CAAACTGCTGTGAACATAGG+AGG + chr1.4:1450808-1450827 MS.gene90735:CDS 45.0%
CCAGTACCTATTACTACCAC+AGG + chr1.4:1450194-1450213 MS.gene90735:CDS 45.0%
CCTCTTAATGCTTTCATGGC+TGG + chr1.4:1451021-1451040 MS.gene90735:CDS 45.0%
CCTGTGGTAGTAATAGGTAC+TGG - chr1.4:1450197-1450216 None:intergenic 45.0%
GCTTAGTGATAGAGATCTCG+AGG + chr1.4:1450405-1450424 MS.gene90735:intron 45.0%
TCTTCAAGTGGTGGTTTAGG+TGG + chr1.4:1449903-1449922 MS.gene90735:CDS 45.0%
! GGAGTTGGGAAATTTTCCAC+CGG + chr1.4:1450711-1450730 MS.gene90735:CDS 45.0%
!! AGAACTTGTTGCAAGAGTCG+AGG + chr1.4:1449990-1450009 MS.gene90735:CDS 45.0%
!! CCAGCCATGAAAGCATTAAG+AGG - chr1.4:1451024-1451043 None:intergenic 45.0%
!! GAACTTGTTGCAAGAGTCGA+GGG + chr1.4:1449991-1450010 MS.gene90735:CDS 45.0%
ACTAGTCCAAATGCTGCTGC+TGG + chr1.4:1450880-1450899 MS.gene90735:CDS 50.0%
AGAGAACCAGAAGCTCCAGA+GGG - chr1.4:1450164-1450183 None:intergenic 50.0%
TAGAGAACCAGAAGCTCCAG+AGG - chr1.4:1450165-1450184 None:intergenic 50.0%
TCAAGTGGTGGTTTAGGTGG+TGG + chr1.4:1449906-1449925 MS.gene90735:CDS 50.0%
TTTACACACCTGAACACCGC+CGG - chr1.4:1450748-1450767 None:intergenic 50.0%
!! AATGATCCCTCTGGAGCTTC+TGG + chr1.4:1450155-1450174 MS.gene90735:CDS 50.0%
AGTCCAAATGCTGCTGCTGG+TGG + chr1.4:1450883-1450902 MS.gene90735:CDS 55.0%
TCCACCGGAGCTGAATTCAC+CGG + chr1.4:1450726-1450745 MS.gene90735:CDS 55.0%
TGAATTCACCGGCGGTGTTC+AGG + chr1.4:1450737-1450756 MS.gene90735:CDS 55.0%
TGAGTTGTTGCTGCCGTTCG+CGG - chr1.4:1450067-1450086 None:intergenic 55.0%
! AGTGGTGGTTTAGGTGGTGG+TGG + chr1.4:1449909-1449928 MS.gene90735:CDS 55.0%
ACCGCCGGTGAATTCAGCTC+CGG - chr1.4:1450733-1450752 None:intergenic 60.0%
ACCGGAGCTGAATTCACCGG+CGG + chr1.4:1450729-1450748 MS.gene90735:CDS 60.0%
CAGCTGCTAAACGCCGCGAA+CGG + chr1.4:1450051-1450070 MS.gene90735:CDS 60.0%
CCAAATGCTGCTGCTGGTGG+TGG + chr1.4:1450886-1450905 MS.gene90735:CDS 60.0%
CCACCACCAGCAGCAGCATT+TGG - chr1.4:1450889-1450908 None:intergenic 60.0%
CGTTCGCGGCGTTTAGCAGC+TGG - chr1.4:1450053-1450072 None:intergenic 65.0%
GCCGGTGAATTCAGCTCCGG+TGG - chr1.4:1450730-1450749 None:intergenic 65.0%
! GGTGGTGGCGAGGGTTCTTC+CGG + chr1.4:1450901-1450920 MS.gene90735:CDS 65.0%
! GGTTCTTCCGGTGGTGGCAG+CGG + chr1.4:1450913-1450932 MS.gene90735:CDS 65.0%
GGCGAGGGTTCTTCCGGTGG+TGG + chr1.4:1450907-1450926 MS.gene90735:CDS 70.0%
GGTGGCGAGGGTTCTTCCGG+TGG + chr1.4:1450904-1450923 MS.gene90735:CDS 70.0%
TTCGCCACCGCTGCCACCAC+CGG - chr1.4:1450923-1450942 None:intergenic 70.0%
! TCTTCCGGTGGTGGCAGCGG+TGG + chr1.4:1450916-1450935 MS.gene90735:CDS 70.0%
!! GCTGCTGCTGGTGGTGGCGA+GGG + chr1.4:1450892-1450911 MS.gene90735:CDS 70.0%
!! TGCTGCTGCTGGTGGTGGCG+AGG + chr1.4:1450891-1450910 MS.gene90735:CDS 70.0%
! GGTGGTGGCAGCGGTGGCGA+AGG + chr1.4:1450922-1450941 MS.gene90735:CDS 75.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 1449897 1451074 1449897 ID=MS.gene90735
chr1.4 mRNA 1449897 1451074 1449897 ID=MS.gene90735.t1;Parent=MS.gene90735
chr1.4 exon 1449897 1450215 1449897 ID=MS.gene90735.t1.exon1;Parent=MS.gene90735.t1
chr1.4 CDS 1449897 1450215 1449897 ID=cds.MS.gene90735.t1;Parent=MS.gene90735.t1
chr1.4 exon 1450707 1451074 1450707 ID=MS.gene90735.t1.exon2;Parent=MS.gene90735.t1
chr1.4 CDS 1450707 1451074 1450707 ID=cds.MS.gene90735.t1;Parent=MS.gene90735.t1
Gene Sequence

>MS.gene90735

ATGAGGTCTTCAAGTGGTGGTTTAGGTGGTGGTGGAATGAACTGTCAAGATTGTGGTAACCAAGCTAAGAAAGATTGTCCACATCTTAGATGTAGAACTTGTTGCAAGAGTCGAGGGTTTCAGTGTCAAACTCATGTGAAAAGTACTTGGGTTCCAGCTGCTAAACGCCGCGAACGGCAGCAACAACTCACGTCGTTGCAGCAACAAAATCAGCAGCAGCAACAACAGTTTCACTCAAGAAGACACAGAGAAAATCAAAATGATCCCTCTGGAGCTTCTGGTTCTCTAACCTGTACTCCAGTACCTATTACTACCACAGGTATATATATGTAGAAATATAAACTTTTGAACGATATACATCAGCTTTCTTTGACTCACTTGTATTTCTTTACAGAAGAGTCGTTGAAAGAGAGAGTATATAAAATGAAAAGATATTACGAGGGTTTGAATTATAGTGTTTAGTTTTTTGATATATAGCTAGGTCAGTGACGAATTTTCAAACTATATAGCTTAGTGATAGAGATCTCGAGGAGAAACTATAGTTTCTATGAATCTATGATAAATAGGATACCTAATTAGTCTATATATTCTATATATCCATTCCTTTCATACTCTATCAACTTAGTTTTTTTCTTTTCTCTCTCTCTTTTTTCTCTTTCTTTTCTTCTGAAGGATGAAAAAATTGTTAGGTAAGTGGTTCTTTCTTTCTTTTAAATTTGAAAATGATAGCTATATAGAAACAATTGAGGTTTCTCTTTTTGAATTTTTTGATTTGGATTTAATTTTTTTTTTTATTTGATTTGAAATTAGGGTTGGAGTTGGGAAATTTTCCACCGGAGCTGAATTCACCGGCGGTGTTCAGGTGTGTAAAAGTGAGTGCAATGGATGCACAAGATGAACGTTATGCTTATCAAACTGCTGTGAACATAGGAGGAAGAGTTTTCAAGGGAATTTTGTATGATCAAGGACTTGAAGGTAGCTACACTAGTCCAAATGCTGCTGCTGGTGGTGGCGAGGGTTCTTCCGGTGGTGGCAGCGGTGGCGAAGGAAATCAACAAAATCTCATAACAACCACGACAACAAATGTTAACAATCCATTTGAGTATTCTTCACTATATCCAGCTCCTCTTAATGCTTTCATGGCTGGTACGCAATTCTTCCCACCTCCAAGATCCTAA

Protein sequence

>MS.gene90735.t1

MRSSSGGLGGGGMNCQDCGNQAKKDCPHLRCRTCCKSRGFQCQTHVKSTWVPAAKRRERQQQLTSLQQQNQQQQQQFHSRRHRENQNDPSGASGSLTCTPVPITTTGLELGNFPPELNSPAVFRCVKVSAMDAQDERYAYQTAVNIGGRVFKGILYDQGLEGSYTSPNAAAGGGEGSSGGGSGGEGNQQNLITTTTTNVNNPFEYSSLYPAPLNAFMAGTQFFPPPRS